CN106539088B - Preparation method of functional sucrose with blood sugar reducing function - Google Patents
Preparation method of functional sucrose with blood sugar reducing function Download PDFInfo
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- CN106539088B CN106539088B CN201610938894.8A CN201610938894A CN106539088B CN 106539088 B CN106539088 B CN 106539088B CN 201610938894 A CN201610938894 A CN 201610938894A CN 106539088 B CN106539088 B CN 106539088B
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Classifications
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7016—Disaccharides, e.g. lactose, lactulose
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Abstract
The invention discloses a preparation method of functional sucrose with a blood sugar reducing function. Clarifying syrup or syrup dissolved by solid sugar, and finely filtering until no precipitate or suspended matter exists to obtain clear syrup; crushing bagasse and/or sugarcane tips, soaking and extracting to obtain an extract; adding colloidal water solution into the extract concentrated solution to prepare extract dispersion solution; adding the extract dispersion into the clear syrup, and stirring uniformly to obtain mixed syrup of the extract and the clear syrup; controlling the ratio of the total mass of the total polyphenols and the flavonoids in the mixed syrup to the mass of the solid substances in the syrup to be more than 0.02 percent; the liquid mixed syrup is transformed into solid cane sugar through crystallization, and the functional cane sugar with the sugar-reducing function with medium granularity or micro crystal is prepared. The invention makes full use of plant resources such as sugarcane, beet and the like, and the hypoglycemic active substance of the product is active plant element, has the characteristics of full nature, greenness, health and safety, is not only suitable for diabetes mellitus people, but also has the effects of prevention and health care for healthy people.
Description
Technical Field
The invention relates to sucrose preparation, in particular to a preparation method of functional sucrose with a sugar-reducing function, belonging to the field of functional sugar production and processing.
Background
Diabetes mellitus is a chronic metabolic disease mainly characterized by hyperglycemia caused by defects in insulin secretion of the body or impaired biological action of the body, or both, and is one of the biggest threats to human health in the 21 st century. According to the seventh edition of Diabetes Atlas data published in the international Diabetes union idf (international Diabetes federation)2015, 4.15 million adults with Diabetes mellitus, 54 million children with type 1 Diabetes mellitus, by 2040 years will rise to 6.42 million, and 6370 to 11970 million dollars per year will be spent on Diabetes therapy. In China, 1.09 million adults have diabetes and are the first place in the world, so the prevention and treatment of diabetes in China become one of the most urgent problems to be solved.
Sucrose (D (+) -Sucrose), formula: c12H22O11Relative molecular mass: 342.3, common white sugar, brown sugar, crystal sugar and cubic sugar are all cane sugar. Since the sugar has pure and long-lasting sweetness and is a natural organic sweetener, the production and the eating have been for thousands of years, and the long-term eating proves that the sugar is a safe natural sweetener, so the sugar is one of the basic food additives for human beings. However, since sucrose is composed of one molecule of glucose and one molecule of fructose, and is easily decomposed into fructose and glucose by digestion in the human body, synthetic or derivative sweeteners are generally consumed by diabetic patients to prevent blood sugar from rising.
In order to solve the problem that the group of diabetes patients can eat sucrose safely, a plurality of sucrose substitutes are available at present, and corresponding sucrose derivatives are prepared by several sucrose modification technologies, such as: chinese patent No. CN1453284 discloses a method for synthesizing sucralose, comprising the steps of adding N, N-dimethylformamide solution into sucrose as a raw material, carrying out ester exchange reaction with ethyl acetate under the catalysis of a sulfate solid acid catalyst or a sulfate solid adsorbed on a polymer carrier to generate sucrose-6-acetate, and carrying out chlorination and alcoholysis reaction on the sucrose-6-acetate to generate sucralose; chinese invention patent CN103497944A discloses a method for preparing sucrose isomerase, which adopts ultrasonic wave crushing technology to prepare cell wall synthetase containing sucrose isomerase activity as catalyst, to convert sucrose into isomaltulose, and needs to activate the strain of sucrose isomerase producing strain in seed culture medium; then transferring the activated strain to a fermentation medium for culture, and carrying out sucrose isomerase production; then, breaking the sucrose isomerase producing strain by ultrasonic waves and collecting broken cells to obtain cell wall fused sucrose isomerase, and then carrying out sucrose isomerization production by the sucrose isomerase. Although the method modifies the sucrose from different technical angles, the sucrose is not suitable for being hydrolyzed in human bodies, so that the sucrose is suitable for people with diabetes to eat, the research results published by the scientific institute 2014 of Wilzmann, Israel in < Natural > journal prove that the artificial sugar substitute disturbs the balance of intestinal flora and finally reduces the capability of the digestive system to metabolize sugar, so that the non-caloric artificial sugar substitute is more likely to cause diabetes and obesity than Natural sugar.
Disclosure of Invention
The invention aims to provide the cane sugar with the effect of reducing blood sugar and the preparation method thereof, which overcome the technical problems of low active component dose effect, poor stability and the like on the basis of not adding additives except the raw materials for preparing the sugar, and the prepared sugar has good color and taste, high bioavailability, high safety, stable property, good effect of reducing blood sugar and convenient eating.
The cane sugar is mainly extracted from sugar cane and beet, China is the third sucrose producing country in the world, the sugar cane and beet contain sugar such as cane sugar and the like, and also contain more than 3 per mill of polyphenol, flavone, cyanidin and a large amount of phytochemicals beneficial to human health, and the phytochemicals have the obvious effect of inhibiting the increase of blood sugar.
Generally, syrups contain one or more of active ingredients, polyphenol, flavone, pigment, phytosterol, policosanol, polysaccharide, oligosaccharide, reducing sugar, protein, amino acid and mineral substance, and the content is more or trace. The bagasse and/or bagasse extract mainly comprises: malflavone, diglycoside, luteolin, apigenin, chlorogenic acid, ferulic acid, gallic acid, protocatechins, and other phenolic acids. The invention is based on that the active factors for reducing blood sugar in the sugar material are the basis of the substance for reducing blood sugar of functional cane sugar, one or more active components are added in the sugar production process, the technology of dispersion and protection of glue solution is adopted to enhance the high dispersibility, stability and bioavailability of the active components of the sugar material, so as to achieve the purpose of inhibiting the rise of blood sugar. The invention fully utilizes sugar resources, has simple process, no pollution and short production period, has the characteristics of full nature, greenness, health and safety, can realize multiple value increase of products in the sucrose industry, and can improve economic benefits and bring social benefits.
The purpose of the invention is realized by the following technical scheme:
a preparation method of functional sucrose with a hypoglycemic function comprises the following steps:
1) clarifying syrup or syrup dissolved by solid sugar, and fine filtering until no precipitate or suspended matter exists to obtain clear syrup;
2) crushing bagasse and/or sugarcane tips, soaking and extracting to obtain an extract; controlling the total mass content of phytosterol, policosanol, tricin, luteolin, apigenin, diglycoside, chlorogenic acid, ferulic acid, gallic acid and protocatechins in the extract to be more than 3.5%;
3) adding colloidal water solution into the extract concentrated solution to prepare extract dispersion solution; the solid mass percentage of the colloid and the extract is 0.01-0.1%;
4) adding the extract dispersion into the clear syrup, and stirring uniformly to obtain mixed syrup of the extract and the clear syrup; controlling the ratio of the total mass of the total polyphenols and the flavonoids in the mixed syrup to the mass of the solid substances in the syrup to be more than 0.02 percent;
5) vacuum crystallizing the mixed syrup, controlling the mass ratio of total polyphenol and flavone in phytosterol, policosanol, tricin, luteolin, apigenin, diglycoside, chlorogenic acid, ferulic acid, gallic acid and protocatechine to the solid content of the syrup to be more than 0.02%, and preparing into microcrystalline or medium-sized functional sucrose with blood sugar lowering function; or adding the extract dispersion into seminal syrup with a stirring speed of more than 90Bx and higher than 60 rpm-120 rpm in a spraying manner, stopping spraying, and continuing stirring for 1-10 min; obtaining the powder sugar with the function of reducing blood sugar;
to further achieve the object of the present invention, preferably, the syrup is liquid sugar with concentration higher than 60 Bx; is prepared from caulis Sacchari sinensis or beet by extracting, clarifying, and concentrating; the solid sugar is the crystallized sugar of sugarcane or beet which is subjected to juice extraction, clarification, concentration, crystallization, honey separation and drying, the purity is 85-99.9%, the moisture content is not higher than 2.0%, and the color value is 100-5000 IU.
Preferably, the solid sugar is dissolved by dissolving the solid sugar into solid with hot water or dilute sugar solution
(1) Crushing bagasse and/or sugarcane tips to 20-150 meshes of granularity;
(2) adding 50-95% ethanol solution with 2-5 times volume of bagasse and/or sugarcane tips for soaking, and filtering for later use.
Preferably, the extract of step 2) is obtained by:
soaking bagasse and/or sugarcane tips in 50-95% ethanol solution with the volume 2-5 times that of the bagasse and/or sugarcane tips for 10-24 hours, performing rough filtration, performing microfiltration on filtrate with the volume of 0.1-1.0 mu m, and concentrating to remove 70-90% of the volume of the ethanol solution; obtaining a concentrated solution;
(3) adding acetic acid with the volume of 0.05-0.1 of the concentrated solution to obtain a mixed solution, separating the mixed solution on a chromatographic column, eluting the column filler with acetic acid, eluting the chromatographic column with ethanol water solution, collecting the eluent, and concentrating in vacuum.
Preferably, the adsorption resin is an AB-8 or D101 or D730 resin.
Preferably, the volume concentration of the ethanol water solution is 30-70%.
Preferably, the extract dispersion is prepared by mixing in a colloidal aqueous medium through a homogenizer.
Preferably, the colloid is gelatin or pectin; the aqueous phase medium of the colloid is prepared by dissolving the colloid in water to prepare 0.2-2% colloid aqueous solution, and storing at 10-25 ℃; the mixing by a homogenizer is that the high-pressure homogenization is carried out by the homogenizer in the water phase medium with protective colloid, and the granularity is less than 1 μm.
Preferably, the pressure of the vacuum crystallization in the step 5) is-0.85 MPa to-0.50 MPa, and the temperature is 60-85 ℃.
Preferably, the amount of syrup actives of step 5) is determined by HPLC-MS.
The vacuum crystallization of step 5) of the present invention comprises the following steps:
1) concentrating the extract and syrup in vacuum to obtain syrup or massecuite with solid content of 60-96 Bx, wherein the concentration pressure is-0.85 Mpa to-0.50 Mpa, and the temperature is 60-85 ℃;
2) putting the syrup with the pressure of more than 60Bx into a vacuum crystallization tank with the pressure of-0.85-0.50 Mpa and the temperature of 60-85 ℃ to crystallize to crystals with the grain diameter of 100-1500 mu m, so as to obtain the crystal sucrose with the function of reducing blood sugar; or crystallizing to obtain microcrystal with the grain diameter of 5-100 mu m to obtain microcrystal sucrose with the function of reducing blood sugar; or adding the extract dispersion liquid into the massecuite with the volume ratio of 95Bx or more in a spraying mode, stirring at 60-120 rpm for 5-10 min, standing and growing the crystals for 3-8 min; obtaining the sucrose powder with the function of reducing blood sugar.
The invention discloses functional sucrose for assisting in reducing blood glucose index, which utilizes total polyphenols, flavones and other phytochemicals in sugarcane to improve the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in a human body, enhance the oxidation resistance of the human body, reduce the activity of Nitric Oxide Synthase (NOS) and ensure that a protective effect can be generated when the human body is externally damaged. The polyphenol, flavone, phytosterol and oligosaccharide can inhibit the activity of disaccharide hydrolase and a-glucosidase in small intestine, block the absorption of sugar, reduce the load of pancreatic island, and achieve the purpose of assisting to stabilize blood sugar. The total polyphenol and flavone are functional phytochemicals obtained by separating, extracting and purifying bagasse or bagasse as raw material, and the purity of the functional phytochemicals is that the content of medicanin or diglycoside and the like in bagasse or bagasse extract is more than 3.5 percent; the syrup is prepared from sugar cane or beet by clarifying, concentrating or dissolving solid sugar to obtain syrup with solid content of more than 60 Bx. The mass ratio of the total polyphenol and flavone content to the solid syrup is controlled to be more than 0.02 percent.
Compared with the prior art, the invention has the following advantages:
1) the invention makes full use of plant resources such as sugarcane, beet and the like, and the hypoglycemic active substance of the product is active plant element, has the characteristics of full nature, greenness, health and safety, is not only suitable for diabetes mellitus people, but also has the effects of prevention and health care for healthy people.
2) The functional sucrose for assisting to reduce the blood sugar index improves the oxidation resistance of a human body and inhibits the activity of disaccharide hydrolase and a-glucosidase in the small intestine through the phytochemicals such as sugar cane polyphenol, flavone and the like, reduces the absorption of sugar and achieves the aim of assisting to stabilize the blood sugar.
3) After the product is eaten, the sucrose in the product can maintain the health of the intestinal flora and the balance of glycogen, and keep good intestinal microecology and the ability of the vigorous digestive system to metabolize sugar.
Detailed Description
The present invention is further described with reference to the following examples, which should be construed as limiting the scope of the invention as claimed.
Example 1
1. Selecting brown sugar with color value of 4500IU in beet sugar factory as raw material, dissolving brown sugar with hot water to obtain 60Bx syrup, filtering to remove precipitate and suspended matter to obtain clear syrup;
2. taking 500g of dry bagasse, and crushing to 120 meshes of granularity; adding 50% (volume concentration) ethanol solution with volume 3 times of that of the bagasse, soaking for 18 hours, coarsely filtering, microfiltering the filtrate by 0.2 mu m, and concentrating to remove 70% ethanol solution to obtain concentrated solution; adding acetic acid with volume of 5% of the concentrated solution, separating the mixed solution with a chromatographic column filled with AB-8 resin, eluting the chromatographic column with 0.1(v/v) acetic acid with volume of 1AB-8, then eluting with 60% (v/v) ethanol water solution, collecting the eluent, vacuum concentrating to 50Bx, determining the content of medicanin 0.23%, apigenin 0.15%, luteolin 0.31%, chlorogenic acid 0.43%, diglycoside 0.22%, ferulic acid 0.96%, caffeic acid 0.71%, protocatechuic acid 0.23%, tricin 0.20%, policosanol 0.27%, the content of total effective components, such as medicanin or diglycoside, is 3.71%, and is more than 3.5%. Others are soluble carbohydrates.
3. Adding 5mL of pectin aqueous solution with mass concentration of 0.1% into 50mL of bagasse extract with concentration of 50Bx to prepare dispersion of pectin and extract, mixing in water phase medium with protective colloid by a homogenizer, and homogenizing to obtain extract dispersion with particle size less than 1 μm;
4. adding 60mL of 50Bx extract dispersion into 5500mL of 60Bx syrup, and stirring; vacuum concentrating the extract and syrup to obtain syrup with solid content of 70Bx, wherein the concentration pressure is-0.75 Mpa and temperature is 83 deg.C;
5. crystallizing the syrup of 70Bx to the particle size of 500-1500 mu m under the pressure of-0.8 Mpa and the temperature of 78 ℃, and cooling to obtain the microcrystalline sucrose with the function of reducing blood sugar.
6. Drying, internal packaging, external packaging, quality inspection and warehousing. The mass ratio of the total polyphenol and flavone content to the solid content of microcrystalline sucrose in this example was 0.034%.
Example 2
1. Selecting sugarcane raw sugar with a color value of 1500IU, dissolving the raw sugar into syrup of 62Bx by using dilute sugar juice, and filtering to remove precipitates and suspended matters to obtain clear syrup;
2. grinding 300g of sugarcane tips and sieving the ground sugarcane tips with a 80-mesh sieve; adding 70% ethanol solution with volume concentration of 4 times of sugarcane tip volume, soaking for 13 hours, coarsely filtering, microfiltering the filtrate by 0.22 μm, and concentrating to remove 80% volume of ethanol solution to obtain concentrated solution; adding acetic acid 6% of the volume of the concentrated solution, separating the mixed solution by a chromatographic column, wherein the column filler is D730 resin, firstly, using 2D730 volume of 0.2(v/v) of acetic acid, then eluting the chromatographic column by using 50% (v/v) of ethanol water solution, collecting eluent, carrying out vacuum concentration to 35Bx, and determining that the extract contains 0.30% of medicanin, 0.29% of diglycoside, 0.25% of apigenin, 0.20% of luteolin, 0.35% of chlorogenic acid, 0.75% of ferulic acid, 0.85% of caffeic acid, 0.13% of epicatechin, 0.25% of tricin, 0.25% of policosanol, 3.62% of total effective component content of medicanin or diglycoside and the like and is more than 3.5%. Others are soluble carbohydrates.
3. Adding 3mL of pectin aqueous solution with the mass concentration of 0.5% into 70mL of sugarcane tips extract concentrated solution with the concentration of 35Bx, and homogenizing in a water phase medium with protective colloid by microjet under high pressure to make the medium particle size less than 0.5 μm to prepare extract dispersion;
4. adding 53mL of 35Bx extract dispersion into 3700mL of clear syrup with concentration of 62Bx, and stirring;
5. vacuum concentrating the extract and syrup to obtain syrup with solid content of 75Bx, wherein the concentration pressure is-0.80 Mpa and temperature is 80 deg.C;
6. and (3) putting the syrup with the pressure of more than 75Bx into a vacuum crystallization tank with the pressure of-0.78 Mpa and the temperature of 82 ℃ to crystallize to crystals with the grain diameter of 100-500 mu m, or preparing the crystals into microcrystals with the grain diameter of 5-100 mu m to obtain the microcrystalline sucrose with the function of reducing blood sugar.
5. Separating honey, drying, internally packaging, externally packaging, inspecting quality and warehousing.
The total polyphenol and flavone content/microcrystalline sucrose solids ratio of this example was 0.028%.
Example 3
1. Selecting clear sugar pulp of sugarcane with solid content of 70Bx for later use;
2. 200g of bagasse and 100g of sugarcane tips are crushed to the granularity of 100 meshes; adding 4 times volume of 60% ethanol solution, soaking for 10 hr, coarse filtering, microfiltering the filtrate with 1.0 μm, and concentrating to remove 75% volume of ethanol solution; adding acetic acid with the volume of 7% of the concentrated solution, separating the mixed solution by a chromatographic column, wherein the column filler is D101 macroporous adsorption resin, firstly, using acetic acid with the volume of 0.5v/v of 3D101, then eluting the chromatographic column by using 70% v/v of ethanol water solution, collecting eluent, carrying out vacuum concentration to 42Bx, determining that the extract contains 0.29% of medicanin, 0.25% of apigenin, 0.32% of diglycoside, 0.32% of luteolin, 0.37% of chlorogenic acid, 0.94% of ferulic acid, 0.70% of caffeic acid, 0.27% of protocatechin, 0.15% of tricin, 0.21% of policosanol, 3.82% of total effective component content of medicanin or diglycoside and the like, and is more than 3.5%.
3. Adding 4mL of 0.4% pectin aqueous solution into 50mL of 42Bx Saccharum sinensis extract concentrated solution, and homogenizing under high pressure in water phase medium with protective colloid to obtain extract dispersion;
4. vacuum concentrating the extract and syrup to obtain sugar paste with solid content of 95Bx, wherein the concentration pressure is-0.85 Mpa and temperature is 76 deg.C;
adding 50mL42Bx extract dispersion into 2300mL95Bx massecuite in spray form, stirring at 120rpm for 10min, standing for crystal growth for 5 min; obtaining the sucrose powder with the function of reducing blood sugar.
5. Drying, internal packaging, external packaging, quality inspection and warehousing.
The total polyphenol and flavone content/microcrystalline sucrose solids ratio of this example was 0.036%.
The invention utilizes the process of combining high-pressure homogenization and colloid protection dispersion, increases the dispersibility of the bagasse and sugarcane tip active components, and has the advantages of small heat loss to the sugarcane active components, high bioavailability, strong coloring and good stability. The invention utilizes polyphenol, flavone and oligosaccharide in sugarcane to inhibit the activity of disaccharide hydrolase and a-glucosidase in the small intestine, blocks the absorption of sugar, reduces the load of pancreatic islets and achieves the aim of assisting to stabilize blood sugar. Meanwhile, the polyphenol and the flavone are utilized to improve the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the human body, enhance the self oxidation resistance of the human body and generate a protective effect when the organism is externally damaged. The sugar 1 obtained in examples 1, 2 and 3 was used#、2#、3#Sample No. 2 for sugar tolerance in miceThe quantitative experiments were as follows:
the experimental method comprises the following steps: auxiliary hypoglycemic action-1.4.2 sugar tolerance of health department' technical Specification for testing and evaluating health food (2003 edition)
1. Material
1.1 test substance: glucose and white granulated sugar, 3 parts of the sugar product (colored sugar-1 #, 2#, 3 #).
1.2 animals: the SPF-level Kunming healthy male mice with the weight of 26-28 g are provided by the Guangdong province medical experimental animal center (animal production license number: SCXK (Guangdong) 2008-. The pellet feed is also provided by the medical experimental animal center of Guangdong province.
1.3 animal groups
(1)2 control group (glucose, white granulated sugar)
(2)3 test article groups (colored sugar-1 #, 2#, 3#)
Each group had 12 animals. Blood glucose levels between groups differed by no more than 1.1 mmol/L.
1.4 animal laboratory conditions: the animal laboratory is SPF grade, and the experimental animals use license numbers: SYXK (Yue) 2013-. The room temperature is 20-25 ℃, and the humidity is 40-70%.
1.5 doses and methods of administration
The experiment sets that the glucose load is 2g/Kg and the white granulated sugar load is 2 g/Kg; the amount of the colored sugar is 2 g/Kg. Each group of animals was gavaged with the corresponding test substance at a volume of 20 mL/Kg.
1.6 Instrument: rapid blood glucose meter (American strong)
2. Observation index and experimental method
2.1 sugar tolerance
Experiment mice in each group are fasted and are not forbidden to be watered for 15 hours, blood is taken from tail ends of the mice, and glucose, white granulated sugar and 3 parts of colored sugar samples are respectively given in a gastric perfusion mode. Blood was collected from the tail end of the mouse several times at 30, 60, and 120min after feeding, and the blood glucose level was measured using a rapid glucometer (forced induction in the United states). The area under the blood glucose curve within 2h after each group was calculated according to the following formula.
AUC ═ 0.5/2+ (30min glucose +60min glucose) × 0.5/2+ (60min glucose +120min glucose)/2.
2.2 statistics: data are expressed as-x ± s. Analysis of variance was performed with statistical software.
3. Results of the experiment
The results of the fasting glucose tolerance average test after 7 days of administration of 2.00g/Kg BW of each of the examples are shown in Table 1 below.
TABLE 1 blood glucose Change after sample administration in test mice
The experimental results show that: after 2.00g/Kg BW of colored sugar is orally given to male mice of SPF-grade Kunming species for 7 days, fasting blood sugar and sugar tolerance tests are respectively determined, and the calculation result of the glycemic index shows that: the glycemic index is lower than that of white granulated sugar produced by modern sugar manufacturing enterprises, especially lower than that of glucose. The blood glucose levels of the test samples 1#, 2#, and 3# were maintained at 5.3 or less for the mice 2 hours after the meal. The sugar product obtained by the invention has the functions of inhibiting the rise of blood sugar and keeping the blood sugar relatively stable.
Claims (3)
1. A preparation method of functional sucrose with a hypoglycemic function is characterized by comprising the following steps:
(1) selecting brown sugar with color value of 4500IU as raw material, dissolving brown sugar with hot water to obtain syrup of 60Bx, filtering to remove precipitate and suspended substances to obtain clear syrup;
(2) taking 500g of dry bagasse, and crushing to 120 meshes of granularity; adding 50% (v/v) ethanol solution with volume 3 times of that of the bagasse, soaking for 18 hours, coarsely filtering, microfiltering the filtrate by 0.2 mu m, and concentrating to remove 70% ethanol solution to obtain concentrated solution; adding acetic acid with the volume of 5% of the concentrated solution, separating the mixed solution by a chromatographic column, wherein the column filler is AB-8 resin, firstly, using 0.1(v/v) acetic acid with the volume of 1BV AB-8, then eluting the chromatographic column by using 60% (v/v) ethanol aqueous solution, collecting eluent, concentrating the eluent in vacuum to 50Bx, determining the content of 0.23% of medicanin, 0.15% of apigenin, 0.31% of luteolin, 0.43% of chlorogenic acid, 0.22% of diglycoside, 0.96% of ferulic acid, 0.71% of caffeic acid, 0.23% of protocatechin, 0.20% of tricin, 0.27% of policosanol, the content of total effective components, such as medicanin or diglycoside, and the like, and the content of the total effective components is 3.71% and is more than 3.; others are soluble carbohydrates;
(3) adding 5mL of pectin aqueous solution with mass concentration of 0.1% into 50mL of bagasse extract with concentration of 50Bx to prepare dispersion of pectin and extract, mixing in water phase medium with protective colloid by a homogenizer, and homogenizing to obtain extract dispersion with particle size less than 1 μm;
(4) adding 60mL of 50Bx extract dispersion into 5500mL of 60Bx syrup, and stirring uniformly; vacuum concentrating the extract and syrup to obtain syrup with solid content of 70Bx, wherein the concentration pressure is-0.75 Mpa and temperature is 83 deg.C;
(5) crystallizing the syrup of 70Bx to the particle size of 500-1500 mu m under the pressure of-0.8 Mpa and the temperature of 78 ℃, and cooling to obtain the microcrystalline sucrose with the function of reducing blood sugar; wherein the mass ratio of the total polyphenol and flavone content to the solid content of the microcrystalline sucrose is 0.034%.
2. A preparation method of functional sucrose with a hypoglycemic function is characterized by comprising the following steps:
(1) selecting sugarcane raw sugar with a color value of 1500IU, dissolving the raw sugar into syrup of 62Bx by using dilute sugar juice, and filtering to remove precipitates and suspended matters to obtain clear syrup;
(2) grinding 300g of sugarcane tips and sieving the ground sugarcane tips with a 80-mesh sieve; adding 70% ethanol solution with volume concentration of 4 times of sugarcane tip volume, soaking for 13 hours, coarsely filtering, microfiltering the filtrate by 0.22 μm, and concentrating to remove 80% volume of ethanol solution to obtain concentrated solution; adding acetic acid 6% of the volume of the concentrated solution, separating the mixed solution by a chromatographic column, wherein the column filler is D730 resin, firstly, eluting the chromatographic column by 2BV of acetic acid 0.2(v/v) of the volume of D730, then, eluting by 50% (v/v) of ethanol water solution, collecting eluent, concentrating the eluent in vacuum to 35Bx, determining that the extract contains 0.30% of medicanin, 0.29% of diglycoside, 0.25% of apigenin, 0.20% of luteolin, 0.35% of chlorogenic acid, 0.75% of ferulic acid, 0.85% of caffeic acid, 0.13% of epicatechin, 0.25% of tricin, 0.25% of policosanol, 3.62% of total effective component content of medicanin or diglycoside and the like and is more than 3.5%; others are soluble carbohydrates;
(3) adding 3mL of pectin aqueous solution with the mass concentration of 0.5% into 70mL of sugarcane tips extract concentrated solution with the concentration of 35Bx, and homogenizing in a water phase medium with protective colloid by microjet under high pressure to make the medium particle size less than 0.5 μm to prepare extract dispersion;
(4) adding 53mL of 35Bx extract dispersion into 3700mL of 62Bx clear syrup, and stirring;
(5) vacuum concentrating the extract and syrup to obtain syrup with solid content of 75Bx, wherein the concentration pressure is-0.80 Mpa and temperature is 80 deg.C;
(6) putting the syrup with the pressure of more than 75Bx into a vacuum crystallization tank with the pressure of-0.78 Mpa and the temperature of 82 ℃ to crystallize to crystals with the grain diameter of 100-500 mu m, or preparing the crystals into microcrystals with the grain diameter of 5-100 mu m to obtain the microcrystalline sucrose with the function of reducing blood sugar; wherein the solid ratio of total polyphenol and flavone content/microcrystalline sucrose is 0.028%.
3. A preparation method of functional sucrose with a hypoglycemic function is characterized by comprising the following steps:
(1) selecting clear sugar pulp of sugarcane with solid content of 70Bx for later use;
(2) 200g of bagasse and 100g of sugarcane tips are crushed to the granularity of 100 meshes; adding 4 times volume of 60% ethanol solution, soaking for 10 hr, coarse filtering, microfiltering the filtrate with 1.0 μm, and concentrating to remove 75% volume of ethanol solution; adding acetic acid with the volume of 7% of the concentrated solution, separating the mixed solution by a chromatographic column, wherein the column filler is D101 macroporous adsorption resin, firstly, using acetic acid with the volume of 0.5v/v of 3BV D101, then eluting the chromatographic column by using 70% v/v of ethanol water solution, collecting eluent, carrying out vacuum concentration to 42Bx, determining that the extract contains 0.29% of medicanin, 0.25% of apigenin, 0.32% of diglycoside, 0.32% of luteolin, 0.37% of chlorogenic acid, 0.94% of ferulic acid, 0.70% of caffeic acid, 0.27% of protocatechin, 0.15% of tricin, 0.21% of policosanol, 3.82% of total effective component content of medicanin or diglycoside and the like, and is more than 3.5%;
(3) adding 4mL of 0.4% pectin aqueous solution into 50mL of 42Bx Saccharum sinensis extract concentrated solution, and homogenizing under high pressure in water phase medium with protective colloid to obtain extract dispersion;
(4) vacuum concentrating the extract and syrup to obtain sugar paste with solid content of 95Bx, wherein the concentration pressure is-0.85 Mpa and temperature is 76 deg.C;
(5) adding 50mL of 42Bx extract dispersion into 2300mL of 95Bx massecuite in a spray form, stirring at 120rpm for 10min, standing for crystal growth for 5 min; obtaining sucrose powder with the function of reducing blood sugar; wherein the solid ratio of total polyphenol and flavone content/microcrystalline sucrose is 0.036%.
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