CN106539088A - A kind of preparation method of the feature sucrose with function of polysaccharide - Google Patents
A kind of preparation method of the feature sucrose with function of polysaccharide Download PDFInfo
- Publication number
- CN106539088A CN106539088A CN201610938894.8A CN201610938894A CN106539088A CN 106539088 A CN106539088 A CN 106539088A CN 201610938894 A CN201610938894 A CN 201610938894A CN 106539088 A CN106539088 A CN 106539088A
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- CN
- China
- Prior art keywords
- syrup
- extract
- sugar
- preparation
- sucrose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000005720 sucrose Substances 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
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- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000021096 natural sweeteners Nutrition 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 230000004260 plant-type cell wall biogenesis Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 239000011973 solid acid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 125000000185 sucrose group Chemical group 0.000 description 1
- 150000003445 sucroses Chemical class 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7016—Disaccharides, e.g. lactose, lactulose
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Epidemiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of preparation method of the feature sucrose with function of polysaccharide.The syrup that syrup or sugar cube dissolve is clarified by the method, fine straining extremely without precipitation and float, obtains clear syrup；Bagasse and/or sugarcane are slightly crushed, is soaked, extracted, obtain extract；Colloid aqueous solution is added in extract concentrated solution, extract dispersion liquid is made into；Extract dispersion liquid is added in clear syrup, is stirred, obtain the mixing molasses of extract and clear syrup；In control mixing molasses, total polyphenols and flavone gross mass are more than 0.02% with the ratio of the solid quality of syrup；The mixing molasses of liquid are crystallized to be transformed into solid-state sucrose, is prepared into the feature sucrose with function of polysaccharide of middle granularity or crystallite.The present invention makes full use of the plant resourceses such as Caulis Sacchari sinensis, Radix Betae, and product hypoglycemic activity thing is active plant element, with All Pure Nature it is green, healthy, it is safe the characteristics of, be not only suitable for diabetic population, and also play the role of prevention, health care to healthy population.
Description
Technical field
The present invention relates to it is prepared by sucrose, the preparation method of more particularly to a kind of feature sucrose with function of polysaccharide,
Belong to functional sugar production and processing field.
Background technology
Diabetes be as body defect of insulin secretion or its biological agent are impaired, or both have concurrently and cause with height
Chronic metabolic disease of the blood glucose for principal character, is one of biggest threat of 21 century human health.Join according to international diabetes
The 7th edition of credit union IDF (International Diabetes Federation) publication in 2015《Diabetes Atlas》
Data display, has 4.15 hundred million adult to suffer from diabetes at present, and 540,000 children suffer from type 1 diabetes, will rise to the year two thousand forty
To 6.42 hundred million, annual 6370 hundred million to 11,970 hundred million dollar expenses are on treating diabetes.China has 1.09 hundred million adults to suffer from glycosuria
Disease, occupies the first in the world, therefore diabetes mellitus in China preventing and treating has become one of difficult problem most urgently to be resolved hurrily.
Sucrose (D (+)-Sucrose), chemical formula：C12H22O11, relative molecular mass：342.3, common white sugar, brown sugar,
Crystal sugar, Cube sugar are all sucrose.Sucrose is pure, long because of sweet taste, and is natural organic sweeting agent, produces and edible existing more than one thousand years
History, long-term edible proof, sucrose are safe natural sweeteners, so, it be the basic food additive of the mankind it
One.But, as the composition of sucrose is dehydrated and is formed by a molecule glucose and a molecule Fructose, Jing digestion is easily point in the human body
Solution becomes Fructose and glucose, therefore, to prevent blood glucose rise, diabetic population from generally eating synthesis or derivative sweeting agent.
To solve the problems, such as diabetes patient's group energy safe edible sucrose, many sucrose succedaneums, several sucrose are had now
Modification technology prepares corresponding sucrose derivative, such as：Chinese invention patent CN1453284 discloses a kind of conjunction of sucralose
Into method, with sucrose as raw material, DMF solution is added, in sulfate solid acid catalyst or absorption in high score
There is ester exchange reaction with ethyl acetate under sulfate solid catalytic action on subcarrier, generate cane sugar-6-acetic ester, sugarcane
Jing chloros, alcoholysis reaction generate sucralose to sugar -6- acetass again；Chinese invention patent CN103497944A discloses a kind of sugarcane
The preparation method of sugared isomerase, using ultrasonic disruption technology, prepares the Cell wall synthesis enzyme containing sucrose isomerase activity and makees
For catalyst, it is isomaltulose by sucrose inversion, sucrose isomerase enzyme-producing bacteria need to be carried out in seed culture medium strain work
Change；To cultivate in activated spawn transferred species to fermentation medium again, carry out sucrose isomerase production；Then use ultrasonic disruption sucrose
Isomery enzyme-producing bacteria simultaneously collects smudge cellses, obtains cell wall fusion sucrose isomerase, then with sucrose isomerase carrying out sucrose
Isomerization is produced.Above method, although sucrose is modified from different technical standpoints so as to should not hydrolyze in human body,
Eat so as to be adapted to diabetic population, but exist according to Wiesmann research institute of Israel 2014<Natural>What magazine was delivered
Result of study is proved:Artificial generation sugar can upset the balance of intestinal microbial population, and the final ability for lowering digestive system metabolism sugar causes
The artificial generation sugar of empty calory easily causes diabetes and obesity than natural sugar.
The content of the invention
It is an object of the invention to provide a kind of sucrose with effect of lowering blood sugar and preparation method, former without sugaring
On the basis of additive beyond material, the technical barriers such as active component dose-effect is low, stability is poor, obtained sugar products color and taste are overcome
All good, bioavailability is high, safe, stable in properties, blood sugar decreasing effect is good, instant.
Sucrose is mainly the extraction from Caulis Sacchari sinensis, Radix Betae, and China is the big sugar industry state in third place in the world, Caulis Sacchari sinensis and Radix Betae
In in addition to containing the sugar such as sucrose, also containing a large amount of plants beneficial to health such as more than 3 ‰ polyphenol, flavone, flower cyanogen elements
Element, plays the role of substantially to suppress blood glucose rise in these phytochemicalss.
Generally syrup all includes active component polyphenol, flavone, pigment, plant sterol, policosanol, polysaccharide, oligosaccharide, reduction
Sugar, protein, aminoacid, mineral are therein one or more, and content is more or micro.Bagasse and/or sugarcane slightly extract owner
Including：Wheat flavone, bioside, luteolin, 4',5,7-trihydroxyflavone etc. and the phenol such as chlorogenic acid, ferulic acid, gallic acid, former catechin
Acid.The present invention is the sugar reducing substance basis of feature sucrose based on the hypoglycemic activity factor in sugar material, is increased during sugaring
One or more active component, the technology disperseed using glue and protected are strengthened the high dispersion of sugar material active component, are stablized
Property and bioavailability, reach the purpose for suppressing blood glucose rise, the sugar products of exploitation is not only particularly suitable for diabetic population, and to strong
Kang Renqun also plays the role of prevention, health care.The present invention takes full advantage of sugar material resource, concise in technology, pollution-free, production cycle
It is short, with All Pure Nature it is green, healthy, it is safe the characteristics of, be capable of achieving many times of increments of cane sugar trade product, economic effect can be improved
Benefit can bring social benefit again.
The object of the invention is achieved through the following technical solutions：
A kind of preparation method of the feature sucrose with function of polysaccharide, comprises the following steps：
1) syrup that syrup or sugar cube dissolve is clarified, fine straining extremely without precipitation and float, obtains clear syrup；
2) bagasse and/or sugarcane are slightly crushed, is soaked, extracted, obtain extract；Plant sterol, Puli in control extract
Alcohol, tricin, Wheat flavone, luteolin, 4',5,7-trihydroxyflavone, disaccharide glycoside compound, chlorogenic acid, ferulic acid, gallic acid and former youngster
The mass content summation of theine is more than 3.5%；
3) colloid aqueous solution is added in extract concentrated solution, be made into extract dispersion liquid；The solid of colloid and extract
Amount of substance percentage ratio is 0.01～0.1%；
4) extract dispersion liquid is added in clear syrup, is stirred, obtain the mixing molasses of extract and clear syrup；Control
In mixing molasses processed, total polyphenols and flavone gross mass are more than 0.02% with the ratio of the solid quality of syrup；
5) by mixing molasses vacuum crystallization, control plant sterol, policosanol, tricin, Wheat flavone, luteolin, celery are yellow
Total polyphenols and flavones content and syrup in element, disaccharide glycoside compound, chlorogenic acid, ferulic acid, gallic acid and former catechin
Solid quality ratio is more than 0.02%, is prepared into the feature sucrose with function of polysaccharide of crystallite or middle granularity；Or will extract
Thing dispersion liquid is added in refinery liquor of the mixing speed higher than more than the 90Bx of 60rpm～120rpm with Sprayable, stops spray
Continue 1～10min of stirring after mist；Obtain the pulverized sugar with function of blood sugar reduction；
Further to realize the object of the invention, it is preferable that described syrup refers to liquid sugar of the concentration higher than 60Bx；By sweet
Sugarcane or Radix Betae are extracted, are clarified, concentration gained；Described sugar cube refer to Caulis Sacchari sinensis or Radix Betae Jing extractions of the juice, clarification, concentration, crystallization, point
Honey is dried, and purity is not higher than 2.0% in 85～99.9%, moisture, crystal sugar of the colour in 100～5000IU.
Preferably, the sugar cube dissolving refers to and sugar cube is dissolved into solid content with hot water or dilute sugar liquid
(1) bagasse and/or sugarcane are slightly crushed to into 20～150 mesh of granularity；
(2) 50～95% alcohol solution dippings of bagasse and/or sugarcane slightly 2～5 times of volumes are added, it is standby after filtering.
Preferably, step 2) extract obtained as follows：
By bagasse and/or sugarcane slightly with 50～95% alcohol solution dipping 10～24 hours of 2～5 times of volumes, coarse filtration, filtrate
The concentration Jing after 0.1 μm～1.0 μm microfiltration removes the 70%～90% of volumes of aqueous ethanol；Obtain concentrated solution；
(3) acetic acid of 0.05～0.1 volume of concentrated solution is added, mixed liquor is obtained, post separation, post will be chromatographed on mixed liquor
Filler is adsorbent resin, first uses acetic acid eluting, then with the de- chromatographic column of ethanol aqueous wash, collects eluent, concentrated in vacuo.
Preferably, the adsorbent resin is AB-8 or D101 or D730 resins.
Preferably, the volumetric concentration of the ethanol water is 30～70%.
Preferably, the extract dispersion liquid is that the mixing of Jing homogenizers is made in the aqueous media for have colloid.
Preferably, the colloid is gelatin or pectin；The aqueous media of the colloid is that colloid is soluble in water, is made
0.2%～2% colloid aqueous solution, preserves at 10～25 DEG C；The Jing homogenizers mixing is mutually situated between in the water for having protecting colloid
In matter, Jing homogenizers carry out high pressure homogenize, make granularity be less than 1 μm.
Preferably, step 5) vacuum crystallization pressure be -0.85Mpa～-0.50Mpa, temperature be 60～85 DEG C.
Preferably, step 5) syrup amount of active ingredients determined with HPLC-MS.
Step 5 of the present invention) vacuum crystallization comprise the following steps：
1) extract is concentrated in vacuo to into the syrup or massecuite that solid content is 60～96Bx together with syrup, concentrates pressure
Power be -0.85Mpa～-0.50Mpa, 60～85 DEG C of temperature；
2) it is -0.85-0.50Mpa the syrup of more than 60Bx to be loaded pressure, in the vacuum crystallization tank of 60～85 DEG C of temperature
The crystal that particle diameter is 100～1500 μm is crystallized to, the crystalline saccharose with function of blood sugar reduction is obtained；Or crystallize to particle diameter for 5～
100 μm of crystallite, obtains the crystallite sucrose with function of blood sugar reduction；Or by extract dispersion liquid with Sprayable add 95Bx with
On massecuite in, 60rpm～120rpm stirring 5min～10min, static 3～8min of growing the grain；Obtain with function of blood sugar reduction
Cane sugar powder.
The present invention develops a kind of function sucrose of assisting in reducing blood sugar index, is using the total polyphenols and flavone in Caulis Sacchari sinensis
The activity of superoxide dismutase (SOD), glutathion peroxidase (GSH-Px) in human body is improved Deng phytochemicalss, strengthens people
The oxidation resistance of body itself, while reducing nitricoxide synthase (NOS) activity, makes to produce when body is subject to extrinsic damage
Protective effect.The presence of polyphenol, flavone, plant sterol and oligosaccharide can suppress little enteral disaccharide-hydrolysing enzymes and a- glucosidase
Activity, block to sugar absorption, mitigate islets of langerhans burden, reach the purpose for aiding in steady blood glucose.Total polyphenols used and flavone are
The functional plants element come so that bagasse or sugarcane slightly raw material carry out separation and Extraction purification, its purity is that bagasse or sugarcane are slightly extracted
In thing, the content of tricin or bioside etc. is more than 3.5% extract；Syrup used is to extract clarified, dense from Caulis Sacchari sinensis or Radix Betae
Contracting or syrup of the solid content more than 60Bx obtained in sugar cube dissolving.Control total polyphenols and flavones content and syrup solids quality
Than more than 0.02%.
Compared with prior art, the present invention has advantages below：
1) present invention makes full use of the plant resourceses such as Caulis Sacchari sinensis, Radix Betae, and product hypoglycemic activity thing is active plant element, with complete
Natural green, health, it is safe the characteristics of, be not only suitable for diabetic population, and also have the work of prevention, health care to healthy population
With.
2) the feature sucrose of assisting in reducing blood sugar index of the invention is carried by phytochemicalss such as Caulis Sacchari sinensis polyphenol and flavone
The activity of high human body oxidation resistance and the little enteral disaccharide-hydrolysing enzymes of suppression and a- glucosidase, reduces the absorption to sugar, reaches
To the purpose for aiding in steady blood glucose.
3) after eating the product of the present invention, sucrose therein can safeguard the health of intestinal microbial population and the balance of glycogen, keep
The ability of good intestinal microecology and vigorous digestive system metabolism sugar.
Specific embodiment
With reference to embodiment, the invention will be further described, it should be noted that embodiment is not constituted to this
The restriction of bright claimed scope.
Embodiment 1
1st, select beet-sugar factory colour 4500IU brown sugar (Saccharum Sinensis Roxb.) be raw material, by brown sugar (Saccharum Sinensis Roxb.) with hot water dissolving into 60Bx sugar
Slurry, is filtered to remove precipitation and float, obtains clear syrup；
2nd, 500g bagasses are taken, 120 mesh of granularity is crushed to；Plus 50% (volumetric concentration) ethanol solution of 3 times of bagasse volumes,
Immersion 18 hours, coarse filtration, filtrate concentrate the ethanol solution for removing 70% volume Jing after 0.2 μm of microfiltration, obtain concentrated solution；Add concentration
The acetic acid of 5% volume of liquid, will chromatograph post separation on mixed liquor, column packing is AB-8 resins, first with 0.1 (v/v) of 1AB-8 volumes
Acetic acid, then take off chromatographic column with the ethanol aqueous wash of 60% (v/v), collect eluent, be concentrated in vacuo to 50Bx, determine and extract
Contain tricin 0.23%, 4',5,7-trihydroxyflavone 0.15%, luteolin 0.31%, chlorogenic acid 0.43%, bioside 0.22%, Resina Ferulae in thing
Acid 0.96%, caffeic acid 0.71%, former catechin 0.23%, Wheat flavone 0.20%, policosanol 0.27%, total effective ingredient contain
Amount, total active constituent content such as tricin or bioside is 3.71%, more than 3.5%.Other are soluble-carbohydrate.
3rd, the aqueous pectin solution that 5mL mass concentrations are 0.1%, system are added in the bagasse extract to 50mL concentration for 50Bx
For the dispersion liquid into pectin and extract, the Jing homogenizers mixing in the aqueous media for have protecting colloid, homogenizing granularity are less than 1 μ
M, is made into extract dispersion liquid；
4th, the extract dispersion liquid of 60mL50Bx is added in the clear syrup that 5500mL concentration is 60Bx, is stirred；
Extract is concentrated in vacuo to together with syrup the syrup of solid content 70Bx, concentration pressure is -0.75Mpa, 83 DEG C of temperature；
5th, by the syrup of 70Bx pressure be -0.8Mpa, 500～1500 μm of particle diameter is crystallized at 78 DEG C of temperature, is cooled down
To the crystallite sucrose with function of blood sugar reduction.
6th, dry, interior bag, outsourcing, quality inspection, warehouse-in.The solid content of the present embodiment total polyphenols and flavones content and crystallite sucrose
Mass ratio is 0.034%.
Embodiment 2
1st, select colour in the Caulis Sacchari sinensis raw sugar of 1500IU, raw sugar is dissolved into into the syrup of 62Bx with dilute syrup, is filtered to remove
Precipitation obtains clear syrup with float；
2nd, 300g sugarcanes were slightly crushed into 80 mesh sieves；Plus the volumetric concentration of 4 times of sugarcane slightly volumes is 70% ethanol solution, soaks 13
Hour, coarse filtration, filtrate concentrate removal 80% volume of ethanol solution Jing after 0.22 μm of microfiltration, obtain concentrated solution；Add 6% body of concentrated solution
Long-pending acetic acid, will chromatograph post separation on mixed liquor, column packing is D730 resins, first with the acetic acid of 2D730 volumes 0.2 (v/v), then
Chromatographic column is taken off with the ethanol aqueous wash of 50% (v/v), collect eluent, be concentrated in vacuo to 35Bx, contain lucerne in determining extract
Mu element 0.30%, bioside 0.29%, 4',5,7-trihydroxyflavone 0.25%, luteolin 0.20%, chlorogenic acid 0.35%, ferulic acid
0.75%, caffeic acid 0.85%, epicatechin 0.13%, Wheat flavone 0.25%, policosanol 0.25%, tricin or bioside etc.
Total active constituent content is 3.62%, more than 3.5%.Other are soluble-carbohydrate.
3rd, the pectin water that 3mL mass concentrations are 0.5% is added in the sugarcane slightly extract concentrated solution to 70mL concentration for 35Bx
Solution, in the aqueous media for have protecting colloid, Jing microjets high pressure homogenize is processed, and is made medium granularity less than 0.5 μm, is made into and carries
Take thing dispersion liquid；
4th, the extract dispersion liquid for taking 53mL35Bx is added in the clear syrup that 3700mL concentration is 62Bx, is stirred；
The 5th, extract be concentrated in vacuo to together with syrup the syrup of solid content 75Bx, concentration pressure for-
0.80Mpa, 80 DEG C of temperature；
6th, it is -0.78Mpa the syrup of more than 75Bx to be loaded pressure, crystallizes to particle diameter in the vacuum crystallization tank of 82 DEG C of temperature
For 100～500 μm of crystal, or 5～100 μm of crystallite is made, obtain the crystallite sucrose with function of blood sugar reduction.
5th, purging, drying, interior bag, outsourcing, quality inspection, warehouse-in.
The solid content ratio of the present embodiment total polyphenols and flavones content/crystallite sucrose is 0.028%.
Embodiment 3
1st, select the clear syrup of Caulis Sacchari sinensis of solid content 70Bx standby；
2nd, 200g bagasse 100g sugarcanes are slightly crushed to into 100 mesh of granularity；Plus 60% alcohol solution dipping 10 of 4 times of volumes is little
When, coarse filtration, filtrate concentrate removal 75% volume of ethanol solution Jing after 1.0 μm of microfiltration；The acetic acid of 7% volume of concentrated solution is added, will
Chromatograph post separation on mixed liquor, column packing is D101 macroporous adsorbent resins, first with the acetic acid of the 0.5v/v of 3D101 volumes, then use
The de- chromatographic column of the ethanol aqueous wash of 70%v/v, collects eluent, is concentrated in vacuo to 42Bx, contains tricin in determining extract
0.29%, 4',5,7-trihydroxyflavone 0.25%, bioside 0.32%, luteolin 0.32%, chlorogenic acid 0.37%, ferulic acid 0.94%, coffee
Coffee acid 0.70%, former catechin 0.27%, Wheat flavone 0.15%, policosanol 0.21%, tricin or bioside etc. it is total effectively into
Content is divided to be 3.82%, more than 3.5%.
3rd, the aqueous pectin solution that 4mL concentration is 0.4% is added in the sugarcane slightly extract concentrated solution to 50mL concentration for 42Bx,
In the aqueous media mesohigh homogenizing for having protecting colloid, extract dispersion liquid is made into；
The 4th, extract be concentrated in vacuo to together with syrup the massecuite of solid content 95Bx, concentration pressure for-
0.85Mpa, 76 DEG C of temperature；
The extract dispersion liquid of 50mL42Bx is added in the massecuite of 2300mL95Bx with Sprayable, 120rpm stirrings
10min, static growing the grain 5min；Obtain the cane sugar powder with function of blood sugar reduction.
5th, dry, interior bag, outsourcing, quality inspection, warehouse-in.
The solid content ratio of the present embodiment total polyphenols and flavones content/crystallite sucrose is 0.036%.
The present invention utilizes the technique that high pressure homogenize and colloid protection dispersion phase are combined, and increases bagasse, sugarcane slightly active component
Dispersibility, little to Caulis Sacchari sinensis active component heat loss, bioavailability is high, color strength and good stability.The present invention is using in Caulis Sacchari sinensis
Polyphenol, flavone and oligosaccharide etc. suppress the activity of little enteral disaccharide-hydrolysing enzymes and a- glucosidase, block the absorption to sugar,
Mitigate islets of langerhans burden, reach the purpose for aiding in steady blood glucose.Polyphenol and flavone is utilized to improve superoxide dismutase in human body simultaneously
Enzyme (SOD), glutathion peroxidase (GSH-Px) activity, strengthen human body intrinsic antioxidative ability, make to be subject to outer in body
Protective effect is produced during damaging.Using 1,2,3 gained sugar products 1 of above example#、2#、3#Number sample carries out the resistance to sugar amount reality of mice
Test as follows：
Experimental technique：Ministry of Public Health《Health food is checked and assessment technique specification》The auxiliary hyperglycemic of (version in 2003) is made
With --- 1.4.2 carbohydrate tolerance
1. material
1.1 tested material：Glucose, white sugar, 3 parts of sugar products (having sugar colour -1#, 2#, 3#) of the present invention.
1.2 animal：SPF levels Kunming kind health male white mouse, 26～28g of body weight, by Guangdong Province medical experiment animal
The heart provides (animal productiong licensing number：SCXK (Guangdong) 2008-0002, animal quality certification numbering：NO
44007200006327).Pellet is also provided by Guangdong Medical Lab Animal Center.
1.3 animal packet
(1) 2 matched group (glucose, white sugar)
(2) 3 test sample groups (having sugar colour -1#, 2#, 3#)
Every group of animal 12.Between each group group, blood sugar level difference is not more than 1.1mmol/L.
1.4 Animal Lab. conditions：Animal Lab. is SPF levels, and laboratory animal uses credit number：SYXK (Guangdong)
2013-0011.20～25 DEG C of room temperature, humidity 40～70%.
1.5 dosage and medication
Experiment setting glucose load 2g/Kg, white sand glucose load 2g/Kg；Coloured sugared agent amount is 2g/Kg.Each group animal with
20mL/Kg capacity gavages give corresponding tested material.
1.6 instrument：Fast blood glucose meter (Johnson ＆ Johnson of the U.S.)
2nd, observation index and experimental technique
2.1 resistance to sugar amounts
After water 15h is can't help in the equal fasting of experiment each group mice, blood is taken in mouse tail tip, give Fructus Vitis viniferae respectively in gavage mode
Sugar, white sugar, 3 parts of coloured sugar-like product.Respectively during feed 30,60,120min, blood is taken inferior to mouse tail tip more, using quick
Blood glucose meter (Johnson ＆ Johnson of the U.S.) determines blood glucose value.Each group Area under the curve of blood glucose in 2h after the meal is calculated as follows.
AUC=(fasting glucose+30min blood glucose) * 0.5/2+ (30min blood glucose+60min blood glucose) * 0.5/2+ (60min blood
Sugar+120min blood glucose)/2.
2.2 statistics：Data are represented with-x ± s.Variance analyses are carried out with statistical software.
3. experimental result
Give respectively 2.00g/Kg BW embodiment have sugar colour 7 days after, fasting glucose tolerance average test result see the table below
1。
1 test mice of table is to change of blood sugar after sample
Experimental result explanation：To SPF level Male Kunming strain mice, orally give 2.00g/Kg BW respectively has sugar colour 7 days
Afterwards, fasting glucose and the test of resistance to sugar amount are determined respectively, and glycaemic index result of calculation shows：Glycaemic index is than modern sugar enterprise
The white sugar of production is low, especially low than glucose.Given the test agent 1#, 2#, 3# are kept to the blood glucose value of mice within 2 hours after the meal
5.3 it is following.Illustrate that gained sugar products of the invention has and suppress blood glucose rise, keep blood glucose smoothly to act on relatively.
Claims (10)
1. a kind of preparation method of the feature sucrose with function of polysaccharide, it is characterised in that comprise the following steps：
1) syrup that syrup or sugar cube dissolve is clarified, fine straining extremely without precipitation and float, obtains clear syrup；
2) bagasse and/or sugarcane are slightly crushed, is soaked, extracted, obtain extract；Plant sterol, policosanol, lucerne in control extract
Mu element, Wheat flavone, luteolin, 4',5,7-trihydroxyflavone, disaccharide glycoside compound, chlorogenic acid, ferulic acid, gallic acid and former catechin
Mass content summation is more than 3.5%；
3) colloid aqueous solution is added in extract concentrated solution, be made into extract dispersion liquid；The solid material of colloid and extract
Amount percentage ratio is 0.01～0.1%；
4) extract dispersion liquid is added in clear syrup, is stirred, obtain the mixing molasses of extract and clear syrup；Control is mixed
In conjunction syrup, total polyphenols and flavone gross mass are more than 0.02% with the ratio of the solid quality of syrup；
5) by mixing molasses vacuum crystallization, control plant sterol, policosanol, tricin, Wheat flavone, luteolin, 4',5,7-trihydroxyflavone, two
The solid content of total polyphenols and flavones content and syrup in glycoside compounds, chlorogenic acid, ferulic acid, gallic acid and former catechin
Mass ratio is more than 0.02%, is prepared into the feature sucrose with function of polysaccharide of crystallite or middle granularity；Or extract is disperseed
Liquid is added in refinery liquor of the mixing speed higher than 90Bx～more than the 96Bx of 60rpm～120rpm with Sprayable, stops spray
Continue 1～10min of stirring after mist；Obtain the crystallite Icing Sugar with function of blood sugar reduction.
2. preparation method according to claim 1, it is characterised in that described syrup refers to liquid of the concentration higher than 60～Bx
Body sugar；Extracted, clarify, concentrated gained by Caulis Sacchari sinensis or Radix Betae；Described sugar cube refers to Caulis Sacchari sinensis or Radix Betae Jing extractions of the juice, clarification, dense
Contracting, crystallization, purging are dried, and purity is not higher than 2.0% in 85～99.9%, moisture, crystal sugar of the colour in 100～6000IU.
3. preparation method according to claim 1 and 2, it is characterised in that the sugar cube dissolving is referred to hot water or dilute
Sugar cube is dissolved into liquid sugar sirup of the concentration higher than 60Bx by sugar liquid, standby after filtering.
4. preparation method according to claim 1, it is characterised in that step 2) extract is as follows
Arrive：
(1) bagasse and/or sugarcane are slightly crushed to into 20～150 mesh of granularity；
(2) 50～95% alcohol solution dipping of addition bagasse and/or sugarcane slightly 2～5 times of volumes 10～24 hours, coarse filtration, filtrate
The concentration Jing after 0.1 μm～1.0 μm microfiltration removes the 70%～90% of volumes of aqueous ethanol；Obtain concentrated solution；
(3) acetic acid of 0.05～0.1 volume of concentrated solution is added, mixed liquor is obtained, post separation, column packing will be chromatographed on mixed liquor
For adsorbent resin, acetic acid eluting is first used, then with the de- chromatographic column of ethanol aqueous wash, collects eluent, it is concentrated in vacuo.
5. preparation method according to claim 4, it is characterised in that the adsorbent resin is AB-8 or D101 or D730 trees
Fat.
6. preparation method according to claim 4, it is characterised in that the volumetric concentration of the ethanol water is 30～
70%.
7. preparation method according to claim 1, it is characterised in that the extract dispersion liquid is in the water phase for having colloid
Jing homogenizers mixing in medium is made into.
8. preparation method according to claim 7, it is characterised in that the colloid is gelatin or pectin；The colloid
Aqueous media is that colloid is soluble in water, makes 0.2%～2% colloid aqueous solution, is preserved at 10～25 DEG C；The Jing homogenizing
Machine mixing is that Jing homogenizers carry out high pressure homogenize in the aqueous media for have protecting colloid, makes granularity be less than 1 μm.
9. preparation method according to claim 1, it is characterised in that step 5) vacuum crystallization pressure for-
0.85Mpa～-0.50Mpa, temperature are 60～85 DEG C.
10. preparation method according to claim 1, it is characterised in that step 5) syrup amount of active ingredients surveyed with HPLC-MS
It is fixed.
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