CN115202025A - 具有倾斜照明的显微镜系统 - Google Patents
具有倾斜照明的显微镜系统 Download PDFInfo
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- CN115202025A CN115202025A CN202210382469.0A CN202210382469A CN115202025A CN 115202025 A CN115202025 A CN 115202025A CN 202210382469 A CN202210382469 A CN 202210382469A CN 115202025 A CN115202025 A CN 115202025A
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Images
Classifications
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- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/082—Condensers for incident illumination only
- G02B21/084—Condensers for incident illumination only having annular illumination around the objective
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- G—PHYSICS
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- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
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- G02B21/08—Condensers
- G02B21/10—Condensers affording dark-field illumination
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
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- G—PHYSICS
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- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
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- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
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- G02B21/08—Condensers
- G02B21/082—Condensers for incident illumination only
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/12—Condensers affording bright-field illumination
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/12—Condensers affording bright-field illumination
- G02B21/125—Condensers affording bright-field illumination affording both dark- and bright-field illumination
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
- G02B6/0001—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings specially adapted for lighting devices or systems
- G02B6/0005—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings specially adapted for lighting devices or systems the light guides being of the fibre type
- G02B6/0006—Coupling light into the fibre
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6484—Optical fibres
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/56—Optics using evanescent waves, i.e. inhomogeneous waves
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Microscoopes, Condenser (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB2105232.9A GB2605793B (en) | 2021-04-13 | 2021-04-13 | Microscope system with oblique illumination |
| GB2105232.9 | 2021-04-13 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115202025A true CN115202025A (zh) | 2022-10-18 |
Family
ID=75949391
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210382469.0A Pending CN115202025A (zh) | 2021-04-13 | 2022-04-13 | 具有倾斜照明的显微镜系统 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20220326498A1 (enExample) |
| EP (1) | EP4075181A1 (enExample) |
| JP (1) | JP2022162999A (enExample) |
| CN (1) | CN115202025A (enExample) |
| GB (1) | GB2605793B (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023057348A1 (en) * | 2021-10-05 | 2023-04-13 | Leica Microsystems Cms Gmbh | Sample carrier and method for imaging a sample |
| CN114324436A (zh) * | 2021-12-23 | 2022-04-12 | 江苏大学 | 一种微纳结构的激光加工与实时原位高分辨观测装置 |
| US12422661B2 (en) * | 2023-01-27 | 2025-09-23 | POSTECH Research and Business Development Foundation | Photographing apparatus and photographing method |
| CN119438158B (zh) * | 2024-11-11 | 2025-11-18 | 湖北大学 | 一种基于物镜光轴偏离显微镜主光轴实现超分辨光学显微成像的方法 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102008034137A1 (de) * | 2007-09-28 | 2009-04-02 | Carl Zeiss Microlmaging Gmbh | Mikroskop und Verfahren zum Betreiben eines Mikroskops |
| DE102009037366A1 (de) * | 2009-08-13 | 2011-02-17 | Carl Zeiss Microlmaging Gmbh | Mikroskop, insbesondere zur Messung von Totalreflexions-Fluoreszenz, und Betriebsverfahren für ein solches |
| CN104246582A (zh) * | 2012-04-20 | 2014-12-24 | 浜松光子学株式会社 | 光束整形装置 |
| DE112013002113B4 (de) * | 2012-04-20 | 2019-03-21 | Hamamatsu Photonics K.K. | Strahlformer |
| US9500847B2 (en) * | 2012-10-12 | 2016-11-22 | Spectral Applied Research Inc. | Total internal reflectance fluorescence (TIRF) microscopy across multiple wavelengths simultaneously |
| LU92505B1 (de) * | 2014-07-22 | 2016-01-25 | Leica Microsystems | Verfahren und vorrichtung zum mikroskopischen untersuchen einer probe |
| JP6540823B2 (ja) * | 2015-11-27 | 2019-07-10 | 株式会社ニコン | 顕微鏡、観察方法、及び制御プログラム |
| JP7112686B2 (ja) * | 2018-07-18 | 2022-08-04 | 株式会社エビデント | 標本観察装置 |
| DE102018009056A1 (de) * | 2018-11-12 | 2020-05-14 | Carl Zeiss Microscopy Gmbh | Beschleunigte Verfahren und Vorrichtungen für die dreidimensionale Mikroskopie mit strukturierter Beleuchtung |
| JP7261903B2 (ja) * | 2019-05-06 | 2023-04-20 | エーエスエムエル ネザーランズ ビー．ブイ． | 暗視野顕微鏡 |
| GB2585072B (en) * | 2019-06-27 | 2024-01-24 | Andor Tech Limited | Radiation Delivery apparatus for microscope systems |
-
2021
- 2021-04-13 GB GB2105232.9A patent/GB2605793B/en active Active
-
2022
- 2022-04-08 EP EP22167314.8A patent/EP4075181A1/en active Pending
- 2022-04-12 US US17/718,632 patent/US20220326498A1/en active Pending
- 2022-04-12 JP JP2022065790A patent/JP2022162999A/ja active Pending
- 2022-04-13 CN CN202210382469.0A patent/CN115202025A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| GB202105232D0 (en) | 2021-05-26 |
| GB2605793B (en) | 2025-04-30 |
| EP4075181A1 (en) | 2022-10-19 |
| US20220326498A1 (en) | 2022-10-13 |
| GB2605793A (en) | 2022-10-19 |
| JP2022162999A (ja) | 2022-10-25 |
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