GB2605793B - Microscope system with oblique illumination - Google Patents

Microscope system with oblique illumination Download PDF

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Publication number
GB2605793B
GB2605793B GB2105232.9A GB202105232A GB2605793B GB 2605793 B GB2605793 B GB 2605793B GB 202105232 A GB202105232 A GB 202105232A GB 2605793 B GB2605793 B GB 2605793B
Authority
GB
United Kingdom
Prior art keywords
microscope system
oblique illumination
oblique
illumination
microscope
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
GB2105232.9A
Other languages
English (en)
Other versions
GB202105232D0 (en
GB2605793A (en
Inventor
Oreopoulos John
Sinclair Peter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Andor Technology Ltd
Original Assignee
Andor Technology Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Andor Technology Ltd filed Critical Andor Technology Ltd
Priority to GB2105232.9A priority Critical patent/GB2605793B/en
Publication of GB202105232D0 publication Critical patent/GB202105232D0/en
Priority to EP22167314.8A priority patent/EP4075181A1/en
Priority to JP2022065790A priority patent/JP2022162999A/ja
Priority to US17/718,632 priority patent/US20220326498A1/en
Priority to CN202210382469.0A priority patent/CN115202025A/zh
Publication of GB2605793A publication Critical patent/GB2605793A/en
Application granted granted Critical
Publication of GB2605793B publication Critical patent/GB2605793B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/082—Condensers for incident illumination only
    • G02B21/084—Condensers for incident illumination only having annular illumination around the objective
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/10—Condensers affording dark-field illumination
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/02—Objectives
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/082—Condensers for incident illumination only
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/12—Condensers affording bright-field illumination
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/12—Condensers affording bright-field illumination
    • G02B21/125—Condensers affording bright-field illumination affording both dark- and bright-field illumination
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/0001—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings specially adapted for lighting devices or systems
    • G02B6/0005—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings specially adapted for lighting devices or systems the light guides being of the fibre type
    • G02B6/0006—Coupling light into the fibre
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6484—Optical fibres
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/56—Optics using evanescent waves, i.e. inhomogeneous waves

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Microscoopes, Condenser (AREA)
GB2105232.9A 2021-04-13 2021-04-13 Microscope system with oblique illumination Active GB2605793B (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
GB2105232.9A GB2605793B (en) 2021-04-13 2021-04-13 Microscope system with oblique illumination
EP22167314.8A EP4075181A1 (en) 2021-04-13 2022-04-08 Microscope system with oblique illumination
JP2022065790A JP2022162999A (ja) 2021-04-13 2022-04-12 斜め照明式顕微鏡システム
US17/718,632 US20220326498A1 (en) 2021-04-13 2022-04-12 Microscope system with oblique illumination
CN202210382469.0A CN115202025A (zh) 2021-04-13 2022-04-13 具有倾斜照明的显微镜系统

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB2105232.9A GB2605793B (en) 2021-04-13 2021-04-13 Microscope system with oblique illumination

Publications (3)

Publication Number Publication Date
GB202105232D0 GB202105232D0 (en) 2021-05-26
GB2605793A GB2605793A (en) 2022-10-19
GB2605793B true GB2605793B (en) 2025-04-30

Family

ID=75949391

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2105232.9A Active GB2605793B (en) 2021-04-13 2021-04-13 Microscope system with oblique illumination

Country Status (5)

Country Link
US (1) US20220326498A1 (enExample)
EP (1) EP4075181A1 (enExample)
JP (1) JP2022162999A (enExample)
CN (1) CN115202025A (enExample)
GB (1) GB2605793B (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023057348A1 (en) * 2021-10-05 2023-04-13 Leica Microsystems Cms Gmbh Sample carrier and method for imaging a sample
CN114324436A (zh) * 2021-12-23 2022-04-12 江苏大学 一种微纳结构的激光加工与实时原位高分辨观测装置
US12422661B2 (en) * 2023-01-27 2025-09-23 POSTECH Research and Business Development Foundation Photographing apparatus and photographing method
CN119438158B (zh) * 2024-11-11 2025-11-18 湖北大学 一种基于物镜光轴偏离显微镜主光轴实现超分辨光学显微成像的方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150049376A1 (en) * 2012-04-20 2015-02-19 Hamamatsu Photonics K.K. Beam-shaping device
US20150131142A1 (en) * 2012-04-20 2015-05-14 Hamamatsu Photonics K.K. Beam expander
WO2020224882A1 (en) * 2019-05-06 2020-11-12 Asml Netherlands B.V. Dark field microscope

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102008034137A1 (de) * 2007-09-28 2009-04-02 Carl Zeiss Microlmaging Gmbh Mikroskop und Verfahren zum Betreiben eines Mikroskops
DE102009037366A1 (de) * 2009-08-13 2011-02-17 Carl Zeiss Microlmaging Gmbh Mikroskop, insbesondere zur Messung von Totalreflexions-Fluoreszenz, und Betriebsverfahren für ein solches
US9500847B2 (en) * 2012-10-12 2016-11-22 Spectral Applied Research Inc. Total internal reflectance fluorescence (TIRF) microscopy across multiple wavelengths simultaneously
LU92505B1 (de) * 2014-07-22 2016-01-25 Leica Microsystems Verfahren und vorrichtung zum mikroskopischen untersuchen einer probe
JP6540823B2 (ja) * 2015-11-27 2019-07-10 株式会社ニコン 顕微鏡、観察方法、及び制御プログラム
JP7112686B2 (ja) * 2018-07-18 2022-08-04 株式会社エビデント 標本観察装置
DE102018009056A1 (de) * 2018-11-12 2020-05-14 Carl Zeiss Microscopy Gmbh Beschleunigte Verfahren und Vorrichtungen für die dreidimensionale Mikroskopie mit strukturierter Beleuchtung
GB2585072B (en) * 2019-06-27 2024-01-24 Andor Tech Limited Radiation Delivery apparatus for microscope systems

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150049376A1 (en) * 2012-04-20 2015-02-19 Hamamatsu Photonics K.K. Beam-shaping device
US20150131142A1 (en) * 2012-04-20 2015-05-14 Hamamatsu Photonics K.K. Beam expander
WO2020224882A1 (en) * 2019-05-06 2020-11-12 Asml Netherlands B.V. Dark field microscope

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
METHODS, vol 88, 2015, PEDRO ALMADA ET AL, "PALM and STORM: Into large fields and high-throughput microscopy with sCMOS detectors", pages 109-121 *

Also Published As

Publication number Publication date
GB202105232D0 (en) 2021-05-26
EP4075181A1 (en) 2022-10-19
US20220326498A1 (en) 2022-10-13
GB2605793A (en) 2022-10-19
CN115202025A (zh) 2022-10-18
JP2022162999A (ja) 2022-10-25

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