CN115177641A - 一种用于辅助控制高血压的组合物 - Google Patents

一种用于辅助控制高血压的组合物 Download PDF

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CN115177641A
CN115177641A CN202110370641.6A CN202110370641A CN115177641A CN 115177641 A CN115177641 A CN 115177641A CN 202110370641 A CN202110370641 A CN 202110370641A CN 115177641 A CN115177641 A CN 115177641A
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folic acid
hypertension
probiotics
composition
composition according
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徐希平
张磊
李锦良
于多
陈平
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Shenzhen Ausa Pharmaceutical Co ltd
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Shenzhen Ausa Pharmaceutical Co ltd
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Abstract

本发明涉及一种含有益生菌和叶酸类物质的组合物，其中益生菌选自植物乳杆菌、瑞士乳杆菌、干酪乳杆菌的一种或多种，还可以包括其他益生菌。本发明的优点是：该组合物可以辅助控制高血压，尤其适宜妊娠期女性，可以预防或辅助治疗妊娠高血压，同时也能为孕妇补充所需叶酸等营养素，预防出生缺陷等。

Description

一种用于辅助控制高血压的组合物
技术领域
本发明涉及一种含有益生菌和叶酸类物质的组合物及其用途，属于健康领域。
背景技术
高血压是最常见的慢性心血管疾病，也是许多其它心脑血管病(如冠心病、脑卒中、心衰)重要的危险因素。根据国家心血管病中心发布的《全国高血压控制状况调查》显示，我国高血压患病率已高达23.2％，但治疗率仅为40.7％，控制率更是低至15.3％。据预测，到2025年全球将有10.5亿高血压患者。因此，预防与控制高血压的公共卫生学意义重大。
妊娠高血压是女性在妊娠期间容易发生的一种独特类型的高血压，可对母亲及胎儿产生不同程度的伤害，主要临床表现为高血压、蛋白尿、水肿等症状和体征，血压一般在140/90mmHg以上。妊娠期高血压如果得不到处理和纠正，则有可能发展为先兆子痫、子痫，危及孕妇及胎儿生命，因此需要高度重视和及时处理。但考虑到大多数降压药物均可能对胎儿产生不利影响，一般不适合在妊娠期间服用，为了预防或辅助治疗妊娠高血压，开发相关的功能性保健食品是一个很好的策略。
益生菌(Probiotics)是一类对宿主有益的活性微生物，它们定殖在人体内，能改变宿主某一部位菌群组成，可以是单微生物，亦可以是成份明确的混合微生物。益生菌通过调节宿主黏膜与系统免疫功能或调节肠道内菌群平衡，促进营养吸收，保持肠道健康。本发明人通过研究发现一些特定菌种还对高血压、包括妊娠高血压有一定的辅助治疗作用。
叶酸是一种水溶性B族维生素，孕妇对叶酸的需求量比正常人高4倍。孕早期是胎儿器官系统分化、胎盘形成的关键时期，细胞生长、分裂十分旺盛。此时叶酸缺乏可导致胎儿畸形.如神经管畸形(我国发生率约3.8‰)、无脑儿、脊柱裂等，还可引起早期自然流产。孕中、晚期，母体血容量增加、胎盘发育使得叶酸需要量大增，叶酸不足则易致胎盘早剥、妊娠高血压、巨幼红细胞性贫血，胎儿易发生宫内发育迟缓，早产和出生低体重等，因此女性在怀孕前和怀孕期间补充叶酸十分必要。
目前市场上益生菌产品虽然众多，但基本都属于综合调理作用，缺乏对特定人群的精准应用设计。本发明精选特定益生菌，并与叶酸组合，制成功能性保健食品等，可用于易患高血压的人群(如有高血压家族史的中老年人)，尤其适合用于妊娠期女性人群的血压温和调节，从而有针对性地预防和辅助治疗(妊娠)高血压，很好地满足市场和临床需求，是对现有益生菌或叶酸单一产品的技术改进。
发明内容
本发明采用以下技术方案。
本发明提供的组合物、用途及产品中所用原料均可从市场购得。实施例中所涉及的益生菌的益生菌总量均不小于106CFU/g。
一种组合物，包括：(1)叶酸类物质；(2)益生菌；(3)辅料。
在本发明中，所述的组合物以重量份计，含有叶酸类物质0.1～1份，益生菌100～1000份；优选叶酸类物质0.1～0.8份，益生菌100～600份；更加优选叶酸类物质0.2～0.5份。
在本发明中，所述的份可以是微克、毫克、克、微升、毫升、分升等。
在本发明中，所述的组合物中的叶酸类物质选自选自5-甲基四氢叶酸、甲酰四氢叶酸钙、二氢叶酸、叶酸或叶酸盐的活性代谢物和可在体内释放/生成叶酸类物质中的一种或多种。
在本发明中的组合物，所述益生菌为植物乳杆菌、瑞士乳杆菌、干酪乳杆菌中的一种或多种。
本发明所述的组合物，还包括食用或药用可接受的赋形剂或载体或其混合物。
在本发明中，所述的组合物还可以含有一种或多种益生元，所述益生元选自菊粉、低聚果糖、低聚半乳糖、低聚葡萄糖、低聚木糖、阿拉伯半乳聚糖、葡甘露聚糖、半乳甘露聚糖和/或它们的组合。
在本发明中，所述的组合物剂型为口服制剂，其制备形式包括固体饮料、片剂、胶囊剂、凝胶、粉剂、丸剂、果实饮料、糖棒、酸奶饮料、发酵的乳制品、膳食替代物、冰淇淋、奶酪或奶昔。
本发明提供的组合物具有制备预防或辅助治疗高血压的制品中的用途，尤其适用于预防或辅助治疗妊娠期高血压。
本发明提供的组合物含有创新配方，针对特定人群，即易患高血压的人群，例如有高血压家族史者、有其它心血管危险因素的中老年人，该组合物具有辅助降压，升高叶酸和降低同型半胱氨酸的效果，可以降低心脑血管终点事件发生风险；对于妊娠期高血压女性，具有降低血压、补充叶酸等功效，与高血压药物治疗相比，具有作用温和、无毒副作用、安全性高的特点,兼有预防出生缺陷,调节妊娠期胃肠不适，增强免疫等优点；与单一益生菌、叶酸产品相比，本发明产品还具有两组成分间功能协同作用的优点，并且能够提高服用依从性、减少总体费用，因此具有良好的市场应用前景。
具体实施例
本发明公开了一种组合物,本领域技术人员可以借鉴本文内容,适当改进工艺参数实现。特别需要指出的是,所有类似的替换和改动对本领域技术人员来说是显而易见的，它们都被视为包括在本发明。本发明的方法及应用已经通过较佳实施例进行了描述，相关人员明显能在不脱离本发明内容、精神和范围内对本文所述的方法和应用进行改动或适当变更与组合,来实现和应用本发明技术。
本发明提供的组合物、用途及保健品中所用原药与辅料均可从市场购得。其中，叶酸原料购自帝斯曼(中国)有限公司，5-甲基四氢叶酸购自连云港金康；益生菌购自杜邦营养食品配料(北京)有限公司，实施例中所采用益生菌的好氧菌总量不低于106CFU/g。
实施例1：本发明组合物对肾性(2K1C)高血压伴高同型半胱氨酸模型大鼠血压的影响
试验采用SPF级SD大鼠，雌雄各半，8周龄，购于北京维通利华公司，经过7天检疫和适应性饲养后，纳入试验，试验过程期间，温度控制在18～25摄氏度，湿度控制在45％-75％之间，光照进行12h/12h交替，自由饮水。
采用肾性(2k1c)高血压伴高同型半胱氨酸血症大鼠模型，具体的操作方法如下：
经检疫后合格的雌性SD大鼠，以3％戊巴比妥钠(动物给药剂量30mg/kg)腹腔注射麻醉，背位固定，沿腹中线剪毛，常规消毒，剖腹，长约3cm，分离左肾动脉，以针灸针(雌性采用0.3mm)与左肾动脉平行，用0号线结扎后，抽出针灸针，造成左肾动脉狭窄的模型，再逐步缝合肌肉层和皮肤层，注射青霉素钠10万单位/只，每日1次，连续3天，常规饲养。术后第3周起2k1c造模大鼠开始喂饮0.5％蛋氨酸饮水，以造成高Hcy模型。6周后2k1c造模大鼠血压升高超过140mmHg的为高血压动物模型。另随机选择10只大鼠，手术中只挑出肾动脉并不结扎作为假手术组。
选取血压升高且收缩压稳定在140mmHg以上的且同型半胱氨酸水平升高20％以上者作为高血压伴高同型半胱氨酸造模成功的大鼠进行分组，分组时按照血压和同型半胱氨酸水平进行分层、兼顾体重均一性、将高血压大鼠随机分组(见表1)。按照下表进行给药，并在给药前，给药4周后测定血压和同型半胱氨酸水平。
通过金正均Q值法计算组合物中各组分之间的相互作用：金正均Q值法计算组合物相互作用用如下公式计算Q＝EA+B/(EA+EB-EA×EB)，式中分子代表“实测合并效应”，分母代表“期望合并效应”，Q为两者之比。Q值<0.85时认为两种药物联用为拮抗作用，0.85<Q值<1.15时认为是相加作用，Q值>1.15时认为是协同作用。为满足药理作用关系的分析，将实测值转化为可以直观体现药理作用强弱的效应，计算公式：Ei＝(1-Pi/P模型组)×100％，Pi为各组的实测值，P模型组为模型组的实测值。
结果如下所示：
表1：给药4周对肾性高血压伴高同型半胱氨酸模型大鼠的血压及同型半胱氨酸水平的影响(
Figure BDA0003009161920000041
n＝8～10)
Figure BDA0003009161920000042
Figure BDA0003009161920000051
与假手术组比较，#P<0.05，##P<0.01，与模型组比较*P<0.05，**P<0.01。
通过对SD大鼠进行两肾一夹(2K1C)手术和喂养0.5％的蛋氨酸进行造模后，大鼠血压与同型半胱氨酸水平与假手术比具有明显的上升，说明制造高血压伴高同型半胱氨酸血症大鼠模型成功。与模型组相比，针对血压，各单药组均未产生显著的降血压的效果，其中植物乳杆菌、瑞士乳杆菌组、干酪乳杆菌组表现出一定的降压趋势(P>0.05)；益生菌与叶酸联用后，植物乳杆菌或瑞士乳杆菌与叶酸联用后产生了显著的降血压的作用(P<0.05)；植物乳杆菌、瑞士乳杆菌组、干酪乳杆菌和罗伊式乳杆菌分别与叶酸联用产生的降血压的效果通过与单药效果进行Q值分析，Q值均大于1.15，表现出协同增效的作用。与模型组相比，针对同型半胱氨酸水平，益生菌单独用药组均未产生显著的降Hcy的效果，叶酸0.1mg/kg剂量给药产生了显著的降Hcy的效果(P<0.05)；益生菌与叶酸联用后，均产生了显著的降Hcy的作用(P<0.05)，其中植物乳杆菌或瑞士乳杆菌组与叶酸联用产生的降Hcy的效果通过与单药效果进行Q值分析，Q值均大于1.15，表现出协同增效的作用。由此可见，植物乳杆菌、瑞士乳杆菌组、干酪乳杆菌与叶酸组合用在降压或降Hcy上均产生了显著的协同增效的作用。
实施例2：本发明组合物对雌性SD大鼠妊娠期血压升高的影响
试验采用SD大鼠,10-12周龄，购于北京维通利华公司，经过检疫及适应性饲养7天后，纳入试验，试验期间，温度控制在18～25摄氏度，湿度控制在45％～75％之间，光照进行12h/12h交替，自由饮水。
检疫结束后，采用阴道涂片法确定大鼠的动情周期，将处于动情前期的雌鼠按2:1的比例与正常雄鼠合笼，次晨检查阴道口及垫料，观察是否有阴栓存在，有阴栓者定为怀孕第1天，并将雌、雄鼠分开。
对确认怀孕后的雌性孕鼠进行连续3天的血压测定，选取最后一次血压测定结果≥130mmHg的雌性孕鼠纳入试验中，根据其血压值进行随机分成4组，1个模型组和3个干预组，另取8只正常雌性SD大鼠作为对照组，按照下表分组剂量进行给药至仔鼠出生，灌胃体积按照10ml/kg。确定怀孕给两周后进行血压的检测，仔鼠出生当天采血检测Hcy水平。通过金正均Q值法计算组合物中各组分之间的相互作用。
试验结果:如下所示：
表2：本发明组合物给药对孕鼠血压的影响(
Figure BDA0003009161920000061
n＝7-8)
Figure BDA0003009161920000062
与对照组比较，#P<0.05，##P<0.01，与模型组比较*P<0.05，**P<0.01。
通过对孕鼠的继续观察，组合物组孕鼠所生仔鼠及产后母鼠均正常，不存在出生缺陷，说明本发明组合物用于孕鼠安全有效。
从上述的试验结果得出：与对照组相比,所选择的模型组孕鼠血压有较为明显的升高(P<0.05)，益生菌与5-甲基四氢叶酸的组合具有显著的降低血压和Hcy的作用，通过Q值分析，可见瑞士乳杆菌+干酪乳杆菌+植物乳杆菌与5-甲基四氢叶酸组合在降低血压和Hcy作用方面具有协同增效的作用(对应Q值分别为1.65和1.19，均大于1.15)。

Claims (8)

1.一种组合物，包括：(1)叶酸类物质；(2)益生菌；(3)辅料。
2.根据权利要求1所述的组合物，其特征在于，以重量份计，含有叶酸类物质0.1～1份，益生菌100～1000份；优选叶酸类物质0.1～0.8份，益生菌100～600份；更加优选叶酸类物质0.2～0.5份。
3.根据权利要求1或2任一项所述的组合物，其特征在于，所述的叶酸类物质选自选自5-甲基四氢叶酸、甲酰四氢叶酸钙、二氢叶酸、叶酸或叶酸盐的活性代谢物和可在体内释放/生成叶酸类物质中的一种或多种。
4.根据权利要求1或2任一项所述的组合物，其特征在于，所述益生菌为植物乳杆菌、瑞士乳杆菌、干酪乳杆菌中的一种或多种。
5.根据权利要求1所述的组合物，其特征在于，还可以含有一种或多种益生元，所述益生元选自菊粉、低聚果糖、半乳寡糖、葡寡糖、木寡糖、阿拉伯半乳聚糖、葡甘露聚糖、半乳甘露聚糖和/或它们的组合。
6.根据权利要求1～5任一项所述的组合物，其特征在于，其制备形式包括固体饮料、片剂、胶囊剂、凝胶、粉剂、丸剂、果实饮料、糖棒、酸奶饮料、发酵的乳制品、膳食替代物、冰淇淋、奶酪或奶昔。
7.根据权利要求1～6任一项所述的组合物在制备辅助控制高血压的制品中的用途。
8.根据权利要求7所述的用途，其特征在于，所述的高血压是女性妊娠期高血压。
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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005109175A2 (en) * 2004-05-05 2005-11-17 Lifescape Biosciences Incorporated Vitamin compositions and treatment methods for metabolic diseases and nutrient deficiencies
CN102099044A (zh) * 2008-05-16 2011-06-15 益生菌股份公司 用于治疗高同型半胱氨酸血症的益生细菌的用途
CN105076402A (zh) * 2015-09-25 2015-11-25 齐齐哈尔医学院 一种富含天然叶酸保健酸奶的制备方法
US20180289749A1 (en) * 2015-11-02 2018-10-11 Optibiotix Limited Composition Comprising Lactobacillus Plantarum 2830 (ECGC 13110402)
CN110150526A (zh) * 2019-06-12 2019-08-23 上海紫微健康管理有限责任公司 固体饮料及其制备方法
CN113398151A (zh) * 2021-06-29 2021-09-17 苏州麦若尔生物科技有限公司 一种用于治疗高同型半胱氨酸血症的益生菌与益生元结合发酵乳及其制备方法和用途
CN116473972A (zh) * 2022-01-17 2023-07-25 深圳芙莱特营养与健康有限公司 一种用于降低同型半胱氨酸的组合物

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005109175A2 (en) * 2004-05-05 2005-11-17 Lifescape Biosciences Incorporated Vitamin compositions and treatment methods for metabolic diseases and nutrient deficiencies
CN102099044A (zh) * 2008-05-16 2011-06-15 益生菌股份公司 用于治疗高同型半胱氨酸血症的益生细菌的用途
CN105076402A (zh) * 2015-09-25 2015-11-25 齐齐哈尔医学院 一种富含天然叶酸保健酸奶的制备方法
US20180289749A1 (en) * 2015-11-02 2018-10-11 Optibiotix Limited Composition Comprising Lactobacillus Plantarum 2830 (ECGC 13110402)
CN110150526A (zh) * 2019-06-12 2019-08-23 上海紫微健康管理有限责任公司 固体饮料及其制备方法
CN113398151A (zh) * 2021-06-29 2021-09-17 苏州麦若尔生物科技有限公司 一种用于治疗高同型半胱氨酸血症的益生菌与益生元结合发酵乳及其制备方法和用途
CN116473972A (zh) * 2022-01-17 2023-07-25 深圳芙莱特营养与健康有限公司 一种用于降低同型半胱氨酸的组合物

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
JIAN ZHANG等: "Screening of folate-producing lactic acid bacteria and modulatory effects of folate-biofortified yogurt on gut dysbacteriosis of folate-deficient rats", FOOD FUNCT, vol. 11, 22 July 2020 (2020-07-22), pages 6308 - 6318 *
张建鸿等: "妊娠期高血压疾病患者血浆总同型半胱氨酸水平监测及其干预治疗的研究", 中国妇幼保健, no. 05, 10 February 2008 (2008-02-10), pages 610 - 612 *
张朝当等: "H型高血压2.0—药物研发与脑卒中精准预防", 药学进展, vol. 43, 25 August 2019 (2019-08-25), pages 567 - 575 *
曹佩等: "产叶酸乳酸菌的筛选及对发酵乳的影响", 食品工业科技, no. 11, 31 January 2018 (2018-01-31), pages 108 - 112 *
隋霜等: "高同型半胱氨酸与妊娠高血压疾病的研究进展", 医学综述, no. 06, 20 March 2011 (2011-03-20) *

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