CN115175916B - 加帽rna的制造方法 - Google Patents

加帽rna的制造方法 Download PDF

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Publication number
CN115175916B
CN115175916B CN202180017057.0A CN202180017057A CN115175916B CN 115175916 B CN115175916 B CN 115175916B CN 202180017057 A CN202180017057 A CN 202180017057A CN 115175916 B CN115175916 B CN 115175916B
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China
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reaction
rna
compound
cap
concentration
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CN115175916A (zh
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阿部洋
木村康明
阿部奈保子
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National Research And Development Corp Science And Technology Revitalization Organization
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National Research And Development Corp Science And Technology Revitalization Organization
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Saccharide Compounds (AREA)
CN202180017057.0A 2020-02-28 2021-02-19 加帽rna的制造方法 Active CN115175916B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020032889 2020-02-28
JP2020-032889 2020-02-28
PCT/JP2021/006360 WO2021172204A1 (ja) 2020-02-28 2021-02-19 キャップ化ｒｎａの製造方法

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CN115175916A CN115175916A (zh) 2022-10-11
CN115175916B true CN115175916B (zh) 2025-02-14

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CN202180017057.0A Active CN115175916B (zh) 2020-02-28 2021-02-19 加帽rna的制造方法

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US (1) US12448408B2 (https=)
EP (1) EP4112630B1 (https=)
JP (1) JP7602807B2 (https=)
CN (1) CN115175916B (https=)
WO (1) WO2021172204A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4509608A4 (en) 2022-03-04 2025-10-08 Japan Science & Tech Agency CAPPED RNA AND PRODUCTION METHOD THEREOF, PROTEIN PRODUCTION APPARATUS, AND PROTEIN PRODUCTION METHOD

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017053297A1 (en) * 2015-09-21 2017-03-30 Trilink Biotechnologies, Inc. Compositions and methods for synthesizing 5'-capped rnas

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017053297A1 (en) * 2015-09-21 2017-03-30 Trilink Biotechnologies, Inc. Compositions and methods for synthesizing 5'-capped rnas

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
5’-Terminal chemical capping of spliced leader RNAs;Karolina Piecyk，等;《Tetrahedron Letters 》;第53卷;第4843–4847页 *
STEPINSKI,J.等.Catalytic efficiency of divalent metal salts in dinucleoside 5′,5′-triphosphate bond formation.《collection Symposium Series》.2002,第5卷第154-158页. *
Synthesis and Reactions of Nucleoside 5′-Diphosphate Imidazolide. A Nonenzymatic Capping Agent for 5′-Monophosphorylated Oligoribonucleotides in Aqueous Solution;Hiroaki Sawai，等;《J. Org. Chem.》;第64卷;第 5836-5840页 *

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Publication number Publication date
JP7602807B2 (ja) 2024-12-19
EP4112630A1 (en) 2023-01-04
US12448408B2 (en) 2025-10-21
CN115175916A (zh) 2022-10-11
US20230097172A1 (en) 2023-03-30
EP4112630B1 (en) 2024-10-16
EP4112630A4 (en) 2023-08-09
JPWO2021172204A1 (https=) 2021-09-02
WO2021172204A1 (ja) 2021-09-02

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