CN115154379A

CN115154379A - Skin repair composition

Info

Publication number: CN115154379A
Application number: CN202210585713.3A
Authority: CN
Inventors: 张述耀; 黄逸辉; 潘珍; 过新民; 缪海雄; 李军政; 赵承宽; 罗茜华
Original assignee: Guangzhou Red Cross Hospital (jinan University Faculty Of Medical Science Affiliated Guangzhou Red Cross Hospital)
Current assignee: Guangzhou Red Cross Hospital (jinan University Faculty Of Medical Science Affiliated Guangzhou Red Cross Hospital)
Priority date: 2022-05-27
Filing date: 2022-05-27
Publication date: 2022-10-11

Abstract

The invention belongs to the field of cosmetics, and particularly relates to a skin repair composition, which comprises the following components in part by weight: hydroxypropyl tetrahydropyrane triol, sodium pyrrolidone carboxylate, sclerotium rolfsii gum, tetrahydro methyl pyrimidine carboxylic acid, glutathione, a green tea extract, a thickening agent and water, wherein the sclerotium rolfsii gum is wrapped by hydroxypropyl-beta-cyclodextrin; also provided are methods for preparing the starting materials and methods for preparing the compositions. The invention starts from the principle of skin damage, utilizes the sclerotium rolfsii and the green tea extract to form a surface free radical scavenger, and forms a subcutaneous free radical scavenging effect by matching with the micromolecule osmosis of glutathione, thereby realizing the internal and external oxidation resistance repair and greatly improving the skin repair effect.

Description

Skin repair composition

Technical Field

The invention belongs to the field of cosmetics, and particularly relates to a skin repair composition.

Background

With the existing work pressure, in addition to factors such as environmental pollution, light radiation, food hormone intake and the like, the factors bring skin problems such as dryness, sensitivity, dryness and the like. The root of these problems is skin damage or skin barrier damage, mainly through the release of large amounts of free radicals, which damage the cells, resulting in accelerated skin damage. Therefore, the antioxidant can effectively resist free radicals, thereby effectively relieving cell damage and improving various skin problems.

However, the skin is divided into: epidermis layer, dermis layer, subcutaneous tissue. These three large levels are subdivided into several finer levels. The skin of different layers plays different roles, and when the skin is damaged, the skin of multiple layers is usually damaged; the existing antioxidant has single function and cannot achieve the effects of completely clearing free radicals and repairing skin.

Disclosure of Invention

Aiming at the problems in the prior art, the invention provides a skin repair composition, which starts from the principle of skin damage, utilizes sclerotium rolfsii and green tea extract to form a surface free radical scavenger, and is matched with the micromolecular osmosis of glutathione to form a subcutaneous free radical scavenging effect, thereby realizing internal and external oxidation resistance repair and greatly improving the skin repair effect.

In order to realize the technical purpose, the technical scheme of the invention is as follows:

a skin rejuvenation composition comprising: hydroxypropyl tetrahydropyrane triol, sodium pyrrolidone carboxylate, sclerotium rolfsii gum, tetrahydro methyl pyrimidine carboxylic acid, glutathione, green tea extract and thickening agent.

The hydroxypropyl tetrahydropyrane triol belongs to a naturally extracted xylose derivative, can promote synthesis of integrin, laminin-5, collagen VI I and collagen IV, and form tight connection between dermis and epidermis, so that skin plumpness is improved, more compactness and elasticity are achieved, meanwhile, moisture content between extracellular matrixes can be improved, generation of substances such as proteoglycan is stimulated, and skin repair and skin quality improvement are achieved from inside to outside.

The sodium pyrrolidone carboxylate is a substance naturally existing in the skin, belongs to a derivative of amino acid, has good moisturizing performance, is superior to the traditional moisturizing agents such as glycerin, sorbitol and the like, has an inhibiting effect on the activity of tyrosine oxidase, is matched with the moisturizing agent with extremely high skin adaptability, ensures the absorbability of the sodium pyrrolidone carboxylate in the skin, prevents melanin deposition and ensures the skin whitening effect. The sodium pyrrolidone carboxylate effectively improves the absorbability of the skin, reduces the skin irritation, effectively improves the skin sensitivity and realizes the surface layer whitening from the aspect of the absorption of the epidermis by utilizing the characteristics of natural substances in the skin; meanwhile, the skin quality improvement effect of the hydroxypropyl tetrahydropyrane triol between the dermis and the epidermis realizes the balanced improvement from inside to outside, promotes the skin quality inside and outside the skin while promoting the skin repair, and achieves the effects of whitening and tightening.

The sclerotium rolfsii gum is a natural macromolecular polysaccharide, is also called dextran, has certain thickening property, can form a film-shaped structure, also has strong free radical scavenging capacity, and effectively delays skin aging; sclerotinia sclerotiorum and tetrahydro-methyl pyrimidine carboxylic acid can form stable protection barrier at skin, and tetrahydro-methyl pyrimidine carboxylic acid can complex a plurality of water molecules, forms the protection water film on the cell surface, combines the water content promotion between the cell matrix of hydroxypropyl tetrahydropyrane triol, forms stable moisture protection to the cell, and the effect that sclerotiotinia sclerotiorum can combine with the aqueous phase ensures that the protection water film has the effect of free radical scavenging, scavenges the free radical inside and outside the water film fast. Furthermore, the tetrahydro-methyl pyrimidine carboxylic acid and the sclerotium rolfsii gum can form a stable barrier, stimulate the activity of cells and promote the cells to be stably repaired, and can promote the absorption of the skin cells on the sodium pyrrolidone carboxylate by matching with the adaptability of the sodium pyrrolidone carboxylate and the skin, so that the inhibition effect is permeated into the epidermis, and the whitening effect is further improved.

Glutathione is a tripeptide compound, is a combination of glutamic acid, cystine and glycine, and can efficiently remove free radicals and peroxides generated by skin metabolism, thereby protecting skin tissues and cells and delaying aging. In the use, glutathione's self small molecule characteristic, the cell matrix water content along with skin repair process rises, enter into in the skin epidermis, to eliminating the inside free radical because of the injury produces of skin, the existence of free radical is eliminated from inside at all, combine the powerful free radical scavenging ability of sclerotium rolfsii gum on the surface, when forming stable surface protection effect, the elimination of skin internal and external free radical, the quick restoration of skin has been promoted, combine the active stimulation effect of sclerotium rolfsii gum self, impel cell activity to promote by a wide margin, delay ageing from inside and outside cell tissue aspect.

In conclusion, the technical scheme utilizes the sclerotium rolfsii glue and the glutathione to form antioxidants of different molecular systems, utilizes the micromolecular characteristics of the glutathione to permeate into the skin, forms free radical removal aiming at different layers, combines the strong free radical removal effect of the sclerotium rolfsii glue and the tea polyphenol in the green tea extract, can form a stable free radical protection effect on the epidermis, not only removes free radicals generated in the epidermis repairing process, but also can remove free radicals brought by external factors; the combination of the three components realizes the rapid removal of internal and external free radicals, thereby achieving the effect of improving the skin repair; meanwhile, the skin quality is improved by utilizing the hydroxypropyl tetrahydropyrane triol and the sodium pyrrolidone carboxylate, the activity of cells in the skin is improved, and the self-activity and self-repairing are greatly improved by matching with the cell irritation of the sclerotium rolfsii gum.

The mass ratio of the hydroxypropyl tetrahydropyrane triol in the skin repair composition is 1-3%, and the hydroxypropyl tetrahydropyrane triol can promote the synthesis of collagen and simultaneously convert into hyaluronic acid in the skin.

The mass ratio of the sodium pyrrolidone carboxylate in the skin repair composition is 0.5-1%, the sodium pyrrolidone carboxylate has low irritation, does not influence the skin, has extremely high safety and stability, is a naturally-occurring substance in the skin, and has good absorbability and permeability. Sodium glutamate is used as a raw material of the sodium pyrrolidone carboxylate, and a product is obtained through intramolecular dehydration condensation, but the physiological activity of the product is poor due to the fact that sodium glutamate is obtained through high-pressure pyrolysis at present, the demand of the sodium pyrrolidone carboxylate is large, and in order to solve the problem, the technical scheme further provides a preparation method of the sodium pyrrolidone carboxylate, which comprises the following steps: a1, adding sodium glutamate into a reaction, adding an inorganic dehydrating agent, carrying out a heat preservation reaction for 1-2h under 0.02MPa, and then carrying out a heating cyclization reaction for 3-5h under 0.02MPa to obtain a reaction mixed solution, wherein the mass ratio of the sodium glutamate to the inorganic dehydrating agent is 5; a2, cooling the reaction mixed solution to 80-90 ℃, filtering while the reaction mixed solution is hot, washing the filtrate with ethanol, and then performing spray vacuum drying to obtain the sodium pyrrolidone carboxylate, wherein the temperature of the spray vacuum drying is 80-90 ℃. In the preparation method, the dehydrating agent directly acts in the reaction system to ensure the stable condensation reaction of the sodium glutamate, and simultaneously, water molecules are quickly absorbed and removed, so that the reaction is always kept running in the positive direction, and the yield is improved; the detection shows that the prepared product has purity not lower than 98%, no color and no smell, and the specific rotation is higher than 10 deg.

The mass ratio of the sclerotium rolfsii gum in the skin repair composition is 0.5-2%. The sclerotium rolfsii gum not only has the function of eliminating free radicals, but also has the function of stimulating cell activity, and forms the synergistic effect of repairing skin with other materials. Based on the fact that sclerotium rolfsii gum has irritation to skin cells, the activity of the cells can be increased through the irritation, and the skin sensitivity can be caused by transition irritation, the sclerotium rolfsii gum is included by hydroxypropyl-beta-cyclodextrin; the method for including sclerotium rolfsii gum by hydroxypropyl-beta-cyclodextrin comprises the following steps: b1, dissolving sclerotium rolfsii gum into ethanol, uniformly stirring to form a solution, wherein the concentration of the sclerotium rolfsii gum is 20-50g/L, b2, dissolving hydroxypropyl-beta-cyclodextrin into distilled water to form an inclusion liquid, wherein the concentration of the hydroxypropyl-beta-cyclodextrin is 5-10g/L, b3, adding the inclusion liquid into the solution, uniformly stirring, and performing spray drying to obtain hydroxypropyl-beta-cyclodextrin inclusion sclerotium rolfsii gum, wherein the volume ratio of the inclusion liquid to the solution is 3. The hydroxypropyl-beta-cyclodextrin inclusion of the sclerotium rolfsii gum effectively reduces the exposure of the sclerotium rolfsii gum, plays a role in activity protection, has a slow release effect, ensures the long-acting property of free radical removal, and simultaneously can reduce the stimulation of the sclerotium rolfsii gum on the skin.

The weight percentage of the tetrahydro-methyl pyrimidine carboxylic acid in the skin repair composition is 2-4%.

The mass of the glutathione in the skin repair composition accounts for 0.3-0.8%.

The mass ratio of the green tea extract in the skin repair composition is 4-7%. The extraction solvent of the green tea extract is ethanol, and the extraction process comprises the following steps: c1, flattening and airing the green tea leaves, and crushing the green tea leaves to obtain tea leaf powder; c2, adding the tea powder into absolute ethyl alcohol, stirring at constant temperature for 20-30min under the condition that the material-liquid ratio is 1; the constant-temperature stirring speed is 200-600r/min, the temperature is 40-50 ℃, the adding amount of the sodium chloride is 5 percent of the mass of the tea, the ultrasonic frequency is 40-60kHz, and the temperature is 20-30 ℃; c3, filtering the mixed emulsion for multiple times to obtain a clarified liquid, spraying the clarified liquid into the reaction kettle for deposition, and blowing by nitrogen to obtain a tea extract; the deposition temperature is 30-60 ℃, and the nitrogen purging temperature is 60-70 ℃; the extraction process is simple and easy to implement, the operability is strong, a large amount of chemical reagents are not adopted in the whole extraction process, the activity protection of the tea extract is complete, and the impurity pollution is less.

The thickening agent is xanthan gum, and the mass percentage of the thickening agent in the skin repair composition is 1-2%.

The skin repair composition comprises water in balance.

The preparation method of the skin repair composition comprises the following steps:

step 1, weighing hydroxypropyl tetrahydropyrane triol, sodium pyrrolidone carboxylate, glutathione and green tea extract according to a proportion, adding distilled water, and stirring at a constant temperature for 30min to obtain a mixed solution; the constant-temperature stirring speed is 200-300r/min, and the temperature is 50-60 ℃;

step 2, uniformly mixing sclerotium rolfsii gum and tetrahydro-methylpyrimidine carboxylic acid, adding the mixture into the mixed solution, and continuously stirring the mixture until the mixture is homogeneous to obtain a prefabricated solution; the speed of the continuous stirring is 300-400r/min, and the temperature is 30-50 ℃;

step 3, adding the thickening agent into the prefabricated liquid, stirring for 10min, and cooling to room temperature to obtain a product; the room temperature is 20-30 ℃, and the stirring speed is 100-300r/min.

From the above description, it can be seen that the present invention has the following advantages:

1. the invention starts from the principle of skin damage, utilizes the sclerotium rolfsii and the green tea extract to form a surface free radical scavenger, and forms a subcutaneous free radical scavenging effect by matching with the micromolecular osmosis of glutathione, thereby realizing the internal and external oxidation resistance repair and greatly improving the skin repair effect.

2. According to the invention, the tetrahydro-methyl pyrimidine carboxylic acid can complex a plurality of water molecules to form a protective water film on the cell surface, and the water content between cell matrixes of hydroxypropyl tetrahydropyrane triol is increased, so that stable water protection is formed on cells.

3. The invention utilizes the safety of the materials of the sodium pyrrolidone carboxylate and the glutathione to be matched with the skin homogeneous absorbability, greatly improves the whitening and repairing effects, and simultaneously, the hydroxypropyl tetrahydro-pyranotriol A promotes the synthesis of collagen protein, is beneficial to the self-improvement of skin formation, thereby obtaining the skin improving and repairing effect with balanced internal and external skin types with double effects.

4. The green tea extract is used as a demulcent, free radicals can be rapidly combined, the activity influence of glutathione is guaranteed, the direct action of the glutathione on the inner layer of the skin is guaranteed by matching with the protective barrier of the tetrahydro-methyl pyrimidine carboxylic acid, and the loss is reduced.

Detailed Description

The present invention is described in detail with reference to the attached drawings, but the present invention is not limited to the claims.

Example 1

A skin rejuvenation composition comprising: hydroxypropyl tetrahydropyrane triol, sodium pyrrolidone carboxylate, sclerotium rolfsii gum, tetrahydro methyl pyrimidine carboxylic acid, glutathione, green tea extract, thickening agent and water.

Wherein the mass ratio of the hydroxypropyl tetrahydropyrane triol in the skin repair composition is 1 percent, namely 1g.

The mass ratio of the sodium pyrrolidone carboxylate in the skin repair composition is 0.5%, namely 0.5g, and the preparation method of the sodium pyrrolidone carboxylate comprises the following steps: a1, adding sodium glutamate into a reaction, adding an inorganic dehydrating agent, carrying out heat preservation reaction for 1h under 0.02MPa, and then carrying out heating cyclization reaction for 3-5h under 0.02MPa to obtain a reaction mixed solution, wherein the mass ratio of the sodium glutamate to the inorganic dehydrating agent is 5, the inorganic dehydrating agent is in a solid granular shape, the inorganic dehydrating agent is formed by wrapping cesium zeolite with silicon oxide, the wrapping cesium zeolite with silicon oxide is obtained by soaking the cesium zeolite granules into ethyl silicate ethanol solution with the concentration of 150g/L for 10min, taking out and drying at 80 ℃, then placing into a reaction kettle with the atmosphere water content of 7% for standing for 2h, and finally drying at 300 ℃ for 1h to obtain the wrapping cesium zeolite with silicon oxide, the heat preservation reaction is 120 ℃, and the temperature of the heating cyclization reaction is 160 ℃; and a2, cooling the reaction mixed solution to 80 ℃, filtering while the reaction mixed solution is hot, washing the filtrate with ethanol, and then performing spray vacuum drying to obtain the sodium pyrrolidone carboxylate, wherein the temperature of the spray vacuum drying is 80 ℃. The purity of the prepared product is 98 percent through detection, and the prepared product is colorless and tasteless, and has a left rotation of 10.5 degrees compared with the optical rotation.

The mass ratio of the sclerotium rolfsii gum in the skin repair composition is 0.5 percent, namely 0.5g, and the sclerotium rolfsii gum is subjected to inclusion of the sclerotium rolfsii gum by adopting hydroxypropyl-beta-cyclodextrin; the method for including sclerotium rolfsii gum by hydroxypropyl-beta-cyclodextrin comprises the following steps: b1, dissolving sclerotium rolfsii gum into ethanol, uniformly stirring to form a solution, wherein the concentration of the sclerotium rolfsii gum is 20g/L, b2, dissolving hydroxypropyl-beta-cyclodextrin into distilled water to form an inclusion solution, wherein the concentration of the hydroxypropyl-beta-cyclodextrin is 5g/L, b3, adding the inclusion solution into the solution, uniformly stirring, and performing spray drying to obtain hydroxypropyl-beta-cyclodextrin inclusion sclerotium rolfsii gum, wherein the volume ratio of the inclusion solution to the solution is 3.

The weight ratio of the tetrahydromethylpyrimidine carboxylic acid in the skin repairing composition is 2 percent, namely 2g.

The mass of the glutathione in the skin repair composition accounts for 0.3 percent, namely 0.3g.

The mass ratio of the green tea extract in the skin repair composition is 4%, namely 4g. The extraction solvent of the green tea extract is ethanol, and the extraction process comprises the following steps: c1, flattening and airing the green tea leaves, and crushing the green tea leaves to obtain tea leaf powder; c2, adding the tea powder into absolute ethyl alcohol, stirring for 20min at a constant temperature in a state that the material-liquid ratio is 1; the constant-temperature stirring speed is 200r/min, the temperature is 40 ℃, the adding amount of the sodium chloride is 5 percent of the mass of the tea, the ultrasonic frequency is 40kHz, and the temperature is 20 ℃; c3, filtering the mixed emulsion for multiple times to obtain a clarified liquid, spraying the clarified liquid into the reaction kettle for deposition, and blowing by nitrogen to obtain a tea extract; the deposition temperature was 30 ℃ and the nitrogen purge temperature was 60 ℃.

The thickening agent adopts xanthan gum, and the mass percentage of the thickening agent in the skin repair composition is 1 percent, namely 1g.

The skin repair composition comprises water in balance.

step 1, weighing hydroxypropyl tetrahydropyrane triol, sodium pyrrolidone carboxylate, glutathione and a green tea extract according to a proportion, adding distilled water, and stirring at a constant temperature for 30min to obtain a mixed solution; the constant-temperature stirring speed is 200r/min, and the temperature is 50 ℃;

step 2, uniformly mixing sclerotium rolfsii gum and tetrahydro-methylpyrimidine carboxylic acid, adding the mixture into the mixed solution, and continuously stirring the mixture until the mixture is homogeneous to obtain a prefabricated solution; the speed of continuous stirring is 300r/min, and the temperature is 30 ℃;

step 3, adding the thickening agent into the prefabricated liquid, stirring for 10min, and cooling to room temperature to obtain 100g of a product; the room temperature is 20 ℃, and the stirring speed is 100r/min.

Example 2

The mass ratio of the hydroxypropyl tetrahydropyrane triol in the skin repair composition is 3 percent, namely 3g.

The mass ratio of the sodium pyrrolidone carboxylate in the skin repair composition is 1 percent, namely 1g, and the preparation method of the sodium pyrrolidone carboxylate comprises the following steps: a1, adding sodium glutamate into a reaction, adding an inorganic dehydrating agent, carrying out heat preservation reaction for 2 hours at 0.02MPa, then carrying out heating cyclization reaction for 5 hours at 0.02MPa to obtain a reaction mixed solution, wherein the mass ratio of the sodium glutamate to the inorganic dehydrating agent is 5; and a2, cooling the reaction mixed solution to 90 ℃, filtering while the reaction mixed solution is hot, washing the filtrate with ethanol, and then performing spray vacuum drying to obtain the sodium pyrrolidone carboxylate, wherein the temperature of the spray vacuum drying is 90 ℃. The detection shows that the prepared product has purity of 99%, no color and no smell, and the levorotatory rotation of the specific rotation is 11.2 degrees.

The mass ratio of the sclerotium rolfsii gum in the skin repair composition is 2 percent, namely 2g, and the sclerotium rolfsii gum is wrapped by hydroxypropyl-beta-cyclodextrin; the method for including sclerotium rolfsii gum by hydroxypropyl-beta-cyclodextrin comprises the following steps: b1, dissolving sclerotium rolfsii gum into ethanol, uniformly stirring to form a solution, wherein the concentration of the sclerotium rolfsii gum is 0g/L, b2, dissolving hydroxypropyl-beta-cyclodextrin into distilled water to form an inclusion solution, wherein the concentration of the hydroxypropyl-beta-cyclodextrin is 10g/L, b3, adding the inclusion solution into the solution, uniformly stirring, and performing spray drying to obtain hydroxypropyl-beta-cyclodextrin inclusion sclerotium rolfsii gum, wherein the volume ratio of the inclusion solution to the solution is 3.

The weight ratio of the tetrahydromethylpyrimidine carboxylic acid in the skin repairing composition is 4 percent, namely 4g.

The mass ratio of the glutathione in the skin repair composition is 0.8 percent, namely 0.8g.

The mass ratio of the green tea extract in the skin repair composition is 7%, namely 7g, the extraction solvent of the green tea extract is ethanol, and the extraction process comprises the following steps: c1, flattening and airing the green tea leaves, and crushing the green tea leaves to obtain tea leaf powder; c2, adding the tea powder into absolute ethyl alcohol, stirring for 30min at a constant temperature in a state that the material-liquid ratio is 1; the constant-temperature stirring speed is 600r/min, the temperature is 50 ℃, the adding amount of the sodium chloride is 5 percent of the mass of the tea, the ultrasonic frequency is 60kHz, and the temperature is 30 ℃; c3, filtering the mixed emulsion for multiple times to obtain a clarified liquid, spraying the clarified liquid into the reaction kettle for deposition, and blowing by nitrogen to obtain a tea extract; the deposition temperature was 60 ℃ and the nitrogen purge temperature was 70 ℃.

The thickening agent adopts xanthan gum, and the mass ratio of the thickening agent in the skin repair composition is 2%, namely 2g.

The skin repair composition comprises water in balance.

step 1, weighing hydroxypropyl tetrahydropyrane triol, sodium pyrrolidone carboxylate, glutathione and green tea extract according to a proportion, adding distilled water, and stirring at a constant temperature for 30min to obtain a mixed solution; the constant-temperature stirring speed is 300r/min, and the temperature is 60 ℃;

step 2, after uniformly mixing sclerotium rolfsii gum and tetrahydro-methyl pyrimidine carboxylic acid, adding the mixture into the mixed solution, and continuously stirring the mixture until the mixture is homogeneous to obtain a prefabricated liquid; the speed of continuous stirring is 400r/min, and the temperature is 50 ℃;

step 3, adding the thickening agent into the prefabricated liquid, stirring for 10min, and cooling to room temperature to obtain 100g of a product; the room temperature is 30 ℃, and the stirring speed is 300r/min.

Example 3

A skin rejuvenation composition comprising: hydroxypropyl tetrahydropyrane triol, sodium pyrrolidone carboxylate, sclerotium rolfsii gum, tetrahydro methyl pyrimidine carboxylic acid, glutathione, green tea extract, a thickening agent and water.

The mass ratio of the hydroxypropyl tetrahydropyrane triol in the skin repair composition is 2 percent, namely 2g.

The mass ratio of the sodium pyrrolidone carboxylate in the skin repair composition is 0.8 percent (namely 0.8 g), and the preparation method of the sodium pyrrolidone carboxylate comprises the following steps: a1, adding sodium glutamate into a reaction, adding an inorganic dehydrating agent, carrying out a heat preservation reaction for 2 hours at 0.02MPa, and then carrying out a heating cyclization reaction for 3-5 hours at 0.02MPa to obtain a reaction mixed solution, wherein the mass ratio of the sodium glutamate to the inorganic dehydrating agent is 5; a2, cooling the reaction mixed solution to 85 ℃, filtering while the reaction mixed solution is hot, washing the filtrate with ethanol, and then performing spray vacuum drying to obtain sodium pyrrolidone carboxylate, wherein the temperature of the spray vacuum drying is 85 ℃; the purity of the prepared product is 98 percent through detection, and the prepared product is colorless and tasteless, and the levorotatory rotation of the specific optical rotation is 10.8 degrees.

The mass ratio of the sclerotium rolfsii gum in the skin repair composition is 0.8 percent, namely 0.8g. The sclerotium rolfsii gum is included by hydroxypropyl-beta-cyclodextrin; the method for including the sclerotium rolfsii gum by the hydroxypropyl-beta-cyclodextrin comprises the following steps: b1, dissolving sclerotium rolfsii gum into ethanol, uniformly stirring to form a solution, wherein the concentration of the sclerotium rolfsii gum is 40g/L, b2, dissolving hydroxypropyl-beta-cyclodextrin into distilled water to form an inclusion solution, wherein the concentration of the hydroxypropyl-beta-cyclodextrin is 8g/L, b3, adding the inclusion solution into the solution, uniformly stirring, and performing spray drying to obtain hydroxypropyl-beta-cyclodextrin inclusion sclerotium rolfsii gum, wherein the volume ratio of the inclusion solution to the solution is 3.

The weight percentage of the tetrahydromethylpyrimidine carboxylic acid in the skin repair composition is 3 percent, namely 3g.

The mass of the glutathione in the skin repair composition accounts for 0.5 percent, namely 0.5g.

The mass of the green tea extract in the skin repair composition accounts for 6 percent, namely 6g. The extraction solvent of the green tea extract is ethanol, and the extraction process comprises the following steps: c1, flattening and airing the green tea leaves, and crushing the green tea leaves to obtain tea leaf powder; c2, adding the tea powder into absolute ethyl alcohol, stirring for 25min at a constant temperature under the condition that the material-liquid ratio is 1; the constant-temperature stirring speed is 400r/min, the temperature is 45 ℃, the adding amount of the sodium chloride is 5 percent of the mass of the tea, the ultrasonic frequency is 50kHz, and the temperature is 25 ℃; c3, filtering the mixed emulsion for multiple times to obtain a clarified liquid, spraying the clarified liquid into the reaction kettle for deposition, and blowing by nitrogen to obtain a tea extract; the deposition temperature was 40 ℃ and the nitrogen purge temperature was 65 ℃.

The skin repair composition comprises water in the balance.

The preparation method of the skin repairing composition comprises the following steps:

step 1, weighing hydroxypropyl tetrahydropyrane triol, sodium pyrrolidone carboxylate, glutathione and green tea extract according to a proportion, adding distilled water, and stirring at a constant temperature for 30min to obtain a mixed solution; the constant-temperature stirring speed is 250r/min, and the temperature is 55 ℃;

step 2, uniformly mixing sclerotium rolfsii gum and tetrahydro-methylpyrimidine carboxylic acid, adding the mixture into the mixed solution, and continuously stirring the mixture until the mixture is homogeneous to obtain a prefabricated solution; the speed of the continuous stirring is 350r/min, and the temperature is 40 ℃;

step 3, adding the thickening agent into the prefabricated liquid, stirring for 10min, and cooling to room temperature to obtain 100g of a product; the room temperature is 25 ℃, and the stirring speed is 200r/min.

Performance testing

1. And (3) testing the repair performance:

the comparative example was petrolatum.

The skin repair compositions of examples 1-3 were compared in clinical trials under the following conditions: 120 volunteers aged 20-45 years were randomly divided into 4 groups of 30 individuals each, who were sensitive skin individuals, had symptoms of redness, itching, burning, stinging or redness at the affected area, were not applied to the test area during the test period with any other skin care product applied once a day, morning and evening, respectively, for four weeks. The room temperature is kept at 25 +/-2 ℃, the relative humidity is 40% +/-3%, and the tested person waits for 30min in the environment and then washes the skin for testing.

The comparative example was petrolatum.

And (3) effect comparison: people with improved skin condition and deteriorated skin condition were counted once a week.

	Example 1	Example 2	Example 3	Comparative example
					Improve the number of people	29	34	32	6
Number of people with deterioration	1	0	1	5

The experimental data show that the skin repair composition provided by the application can achieve a good skin improvement effect, is safe and mild, has no irritation, and is suitable for sensitive skin.

2. And (4) moisture retention performance test:

0.2g of each of examples 1 to 3 and comparative examples was sampled and uniformly applied to a 5 cm. Multidot.5 cm glass plate to which a microporous vent tape was attached, and the glass plate was placed in a constant temperature and humidity desiccator, and the mass of the glass plate after being left for 4 hours was measured, and the moisture retention rate was calculated. The moisture retention rate is the ratio of the sample loading mass before and after placement.

The data are detected as follows:

	example 1	Example 2	Example 3	Comparative example
					Moisture retention rate%	93.45	94.38	93.67	80.32

The above data show that the skin rejuvenation compositions provided in examples 1-3 have a moisture retention rate of 90% or more, which fully demonstrates that the present invention forms a protective film on the skin surface by hydration, prevents water evaporation, and helps to strengthen and restore the moisture retention state of the skin.

3. Test for Oxidation resistance

Sequentially adding 2mmol/L FeSO4 3mL and 1mmol/L H2O2 mL into a 25mL cuvette, shaking up, then adding 6mmol/L salicylic acid 3mL, shaking up, heating in a water bath at 37 ℃ for 15min, taking out, and measuring the absorbance; respectively adding 1mL of the solution to be tested, shaking, heating in water bath for 15min, and taking out to test its absorbance.

The comparative example was petrolatum.

The test results were as follows:

	example 1	Example 2	Example 3	Comparative example
					Free radical scavenging rate%	93.81	94.76	94.27	83.23

The results show that the technical scheme has a good free radical scavenging effect, the hydroxyl free radical scavenging rate reaches more than 93%, and the stable free radical scavenging network which is compatible with the inside and the outside is fully formed by the sclerotium rolfsii gum, the glutathione and the green tea extract, so that the antioxidant protective capability of the skin is recovered.

It should be understood that the detailed description of the invention is only for illustrating the invention and is not limited to the technical solutions described in the embodiments of the invention. It will be appreciated by those skilled in the art that the present invention may be modified or substituted equally as well to achieve the same technical result; as long as the use requirements are met, the method is within the protection scope of the invention.

Claims

1. A skin rejuvenation composition characterized by: the method comprises the following steps: hydroxypropyl tetrahydropyrane triol, sodium pyrrolidone carboxylate, sclerotium rolfsii gum, tetrahydro methyl pyrimidine carboxylic acid, glutathione, a green tea extract and a thickening agent, wherein the sclerotium rolfsii gum is wrapped by hydroxypropyl-beta-cyclodextrin.

2. The skin rejuvenation composition as set forth in claim 1 wherein: the mass ratio of the hydroxypropyl tetrahydropyrane triol in the skin repair composition is 1-3%.

3. The skin rejuvenation composition as defined in claim 1 wherein: the mass ratio of the sodium pyrrolidone carboxylate in the skin repairing composition is 0.5-1%.

4. The skin rejuvenation composition as defined in claim 1 wherein: the mass ratio of the sclerotium rolfsii gum in the skin repair composition is 0.5-2%.

5. The skin rejuvenation composition as set forth in claim 1 wherein: the weight percentage of the tetrahydro-methyl pyrimidine carboxylic acid in the skin repair composition is 2-4%.

6. The skin rejuvenation composition as defined in claim 1 wherein: the mass of the glutathione in the skin repair composition accounts for 0.3-0.8%.

7. The skin rejuvenation composition as set forth in claim 1 wherein: the mass of the green tea extract in the skin repair composition is 4-7%.

8. The skin rejuvenation composition as defined in claim 1 wherein: the thickening agent adopts xanthan gum.

9. The skin rejuvenation composition as defined in claim 1 wherein: the skin repair composition comprises water in the balance.

10. The skin rejuvenation composition as set forth in claim 9 wherein: the preparation method of the skin repair composition comprises the following steps:

step 1, weighing hydroxypropyl tetrahydropyrane triol, sodium pyrrolidone carboxylate, glutathione and a green tea extract according to a proportion, adding distilled water, and stirring at a constant temperature for 30min to obtain a mixed solution; the constant-temperature stirring speed is 200-300r/min, and the temperature is 50-60 ℃;

step 2, after uniformly mixing sclerotium rolfsii gum and tetrahydro-methyl pyrimidine carboxylic acid, adding the mixture into the mixed solution, and continuously stirring the mixture until the mixture is homogeneous to obtain a prefabricated liquid; the speed of the continuous stirring is 300-400r/min, and the temperature is 30-50 ℃;