CN115089671B

CN115089671B - Traditional Chinese medicine preparation for preventing and treating goat digestive tract helminthiasis and preparation method thereof

Info

Publication number: CN115089671B
Application number: CN202210653072.0A
Authority: CN
Inventors: 丁俊仁; 魏勇; 肖璐; 王利; 康润敏; 叶勇刚; 于吉锋; 俄木曲者; 李强; 李春; 李兴玉; 吴学婧; 毛丛剑; 林毅; 谢晶; 曹冶
Original assignee: Sichuan Animal Science Academy
Current assignee: Sichuan Animal Science Academy
Priority date: 2022-06-09
Filing date: 2022-06-09
Publication date: 2023-07-28
Anticipated expiration: 2042-06-09
Also published as: CN115089671A

Abstract

The invention provides a traditional Chinese medicine preparation for preventing and treating goat parasitic diseases, which comprises the following components in mass percent: 10-20 parts of carpesium, 30-50 parts of betel nut, 30-50 parts of spina gleditsiae, 10-20 parts of semen pharbitidis, 30-50 parts of dark plum, 10-20 parts of mirabilite, 20-30 parts of selfheal, 20-30 parts of semen plantaginis, 8-15 parts of bighead atractylodes rhizome, 8-15 parts of radix codonopsis, 8-15 parts of fructus aurantii, 8-15 parts of platycodon grandiflorum, 8-15 parts of medicated leaven, 20-30 parts of hawthorn, 20-30 parts of malt and 20-30 parts of liquorice. Can obviously reduce the incidence rate of the goat parasitic disease, has no toxic or side effect and is safe to use.

Description

Traditional Chinese medicine preparation for preventing and treating goat digestive tract helminthiasis and preparation method thereof

Technical Field

The invention belongs to the technical field of veterinary medicines, and in particular relates to a traditional Chinese medicine preparation for preventing and treating goat digestive tract helminthiasis.

Background

The goat has very high nutritive value, can fully meet the demands of people on nutrition, and along with the rapid improvement of the quality of life and the level of people, the goat meat becomes an indispensable food material on dining tables of people. The frequent occurrence of the parasitic disease seriously affects the quality of the goats in captivity, so that the economic benefit of the goats in captivity is reduced, and the health of human beings is also endangered. Therefore, the goat parasitic disease must be strictly controlled.

Digestive tract helminthiasis is the most popular of sheep parasitosis, and is the type of parasitosis with the greatest harm to sheep, and the prevention and treatment of the type of parasitosis are mainly dependent on medicines at present. The popularity of drug-resistant insect strains is caused by excessive abuse of antiparasitic western medicines, single application and the like. Traditional western medicines can not completely remove parasites in goats, and traditional Chinese medicines are not easy to cause drug resistance of insect strains due to the characteristics of multiple targets, multiple effective components, multiple action ways and the like. The betel nut and the omphalia in the traditional antiparasitic traditional Chinese medicine have certain toxicity, which restricts the practical application of the betel nut and the omphalia.

Disclosure of Invention

Aiming at the technical problems, the invention provides a traditional Chinese medicine preparation for preventing and treating goat digestive tract helminthiasis, which can obviously reduce the incidence rate of goat digestive tract helminthiasis, has no toxic or side effect and is safe to use.

In order to achieve the purpose of the invention, the technical scheme adopted by the invention is as follows:

a traditional Chinese medicine preparation for preventing and treating goat digestive tract helminthiasis comprises the following components in mass: 10-20 parts of carpesium, 30-50 parts of betel nut, 30-50 parts of spina gleditsiae, 10-20 parts of semen pharbitidis, 30-50 parts of dark plum, 10-20 parts of mirabilite, 20-30 parts of selfheal, 20-30 parts of semen plantaginis, 8-15 parts of bighead atractylodes rhizome, 8-15 parts of radix codonopsis, 8-15 parts of fructus aurantii, 8-15 parts of platycodon grandiflorum, 8-15 parts of medicated leaven, 20-30 parts of hawthorn, 20-30 parts of malt and 20-30 parts of liquorice.

Preferably, it comprises, by mass: 10-15 parts of carpesium, 40-50 parts of betel nut, 40-50 parts of spina gleditsiae, 10-15 parts of semen pharbitidis, 40-50 parts of dark plum, 10-15 parts of mirabilite, 20-25 parts of selfheal, 20-25 parts of semen plantaginis, 8-10 parts of bighead atractylodes rhizome, 8-10 parts of radix codonopsis, 10-15 parts of fructus aurantii, 10-15 parts of platycodon grandiflorum, 10-15 parts of medicated leaven, 20-25 parts of hawthorn, 20-25 parts of malt and 25-30 parts of liquorice.

The carpesium carpinum, spina gleditsiae, betel nut, semen pharbitidis and dark plum in the prescription are monarch drugs, and the carpesium carpinum and betel nut have the effects of killing parasites and removing food retention and are used for preventing and treating ascariasis, enterobiasis and taeniasis; spina Gleditsiae has effects of detumescence, expelling pus, and killing parasite; the semen pharbitidis can stimulate intestinal tract, promote intestinal peristalsis, counteract accumulation and purgation, and kill parasites; dark plum is sour, and sour can produce ascarid. The mirabilite is used as a ministerial drug and has the effects of clearing heat, moistening dryness, purging and relaxing bowels; spica Prunellae and semen plantaginis are added for heat clearing and fire purging, and diuresis promoting; the bighead atractylodes rhizome, the dangshen, the bitter orange and the platycodon root are used as adjuvants, the bighead atractylodes rhizome, the dangshen and the bitter orange are used for strengthening the spleen and tonifying qi, and the platycodon root is matched with the bitter orange to relieve chest stuffiness and regulate qi so as to strengthen the body resistance; and medicated leaven, hawthorn and malt are added for strengthening spleen and removing food retention; glycyrrhrizae radix has effects of regulating stomach and regulating the functions of the medicines.

Preferably, the mass ratio of the carpesium, the betel nut, the pharbitis seed, the spina gleditsiae and the dark plum is 1:4-5:1:4-5:4-5. Ensures that the toxic and side effects of the carpesium and the pharbitis seed are the lowest.

The invention also provides a preparation method of the traditional Chinese medicine preparation for preventing and treating goat digestive tract helminthiasis, which comprises the following steps:

A. weighing all the medicinal materials except the mirabilite according to the formula proportion, crushing, soaking the medicinal materials with 10 times of 70% ethanol overnight, heating and reflux-extracting for 3 times at 80 ℃, and merging the extracting solutions;

B. concentrating the extractive solution, drying under reduced pressure or spray drying to obtain dry product, and pulverizing into fine powder A;

C. weighing Mirabilitum according to the formula proportion, grinding into powder, mixing with fine powder A, mixing with pharmaceutically acceptable medicinal adjuvants, adding 70% ethanol, adding ethanol 10% of the material mass, and mixing to obtain granule.

Preferably, in the step B, the extracting solution is concentrated to a concentrated solution with a relative density of 1.03-1.10 at 50-60 ℃.

Preferably, the medicinal materials are crushed and sieved by a 100-mesh sieve.

Preferably, the heat reflux time is 40min each.

Preferably, the pharmaceutically acceptable pharmaceutical excipients are selected from any one or more of starch, microcrystalline cellulose, sucrose, aspartame, dextrin, lactose, powdered sugar, glucose, sodium chloride, sodium carboxymethyl cellulose, crosslinked polyvinylpyrrolidone, magnesium stearate, micro powder silica gel, vitamin C, cysteine, citric acid and sodium sulfite.

The invention has the beneficial effects that:

1. in the prescription of the invention, the carpesium, betel nut, spina gleditsiae, semen pharbitidis and dark plum are taken as monarch drugs to expel parasites, the dosage of carpesium and semen pharbitidis is strictly controlled, and the toxicity is avoided. Spina gleditsiae and dark plum are nontoxic, and the spina gleditsiae has the effects of detumescence, expelling pus and killing insects; dark plum is sour, and sour can produce ascarid. Semen Pharbitidis can stimulate intestinal tract, promote intestinal peristalsis, and counteract accumulation and purgation; the Mirabilitum and the Chinese honeylocust spine are used for being more beneficial to expelling the parasites, and the common selfheal fruit-spike and the plantain seed are used for assisting, so that the effects of clearing heat, purging pathogenic fire and promoting diuresis are achieved; the bighead atractylodes rhizome, the dangshen, the bitter orange and the platycodon root are used as adjuvants, the middle-jiao warming and qi regulating are used for strengthening the body resistance, the medicated leaven, the hawthorn and the malt are used as auxiliary materials, the spleen is strengthened, the food retention is eliminated, and the liquorice is used as the guide, so that the Chinese medicinal composition has the effects of harmonizing the middle-jiao and coordinating the medicines. The compound has scientific and reasonable compatibility, and the synergistic effect of expelling parasites, promoting diuresis, promoting qi and strengthening spleen is obvious, has obvious curative effect on goat parasitic diseases, and is especially aimed at treating gastrointestinal parasites.

2. The common selfheal fruit-spike and the plantain seed not only can drain and promote diuresis, but also have the effects of clearing heat and detoxicating and harmonizing the toxicity of the insect repellent, and meanwhile, the common atractylodes rhizome, the pilose asiabell root, the bitter orange and the platycodon root are used as adjuvants for warming middle-jiao and regulating qi to strengthen the body resistance, and the medicated leaven, the hawthorn and the malt are used as adjuvants for strengthening the spleen and removing food retention, so that the digestion is promoted, the irritation of the medicine to the intestines and the stomach is reduced, and the adverse reaction is reduced and avoided, so that the insect repellent is efficient, low-toxic, long-acting and environment-friendly.

3. The compound medicine is prepared into the granules, and is convenient to mix into feed for feeding, the preparation method is to soak the compound medicine with 10 times of 70% ethanol overnight, and the total saponin of the medicine is the highest by selecting 10 times of 70% ethanol, so that the ethanol waste is avoided; the medicinal material is crushed and sieved by a 100-mesh sieve, can be better contacted with ethanol, and has the best diffusion speed; leaching for 40min, wherein the leaching rate is highest; the mirabilite is directly mixed with the powder obtained by leaching and drying without leaching, and then the granules are prepared.

Detailed Description

The invention will be further described by the following examples for the purpose of more clearly and specifically describing the object of the invention. The following examples are only for specific illustration of the implementation method of the present invention and do not limit the protection scope of the present invention.

Example 1

A traditional Chinese medicine preparation for preventing and treating goat digestive tract helminthiasis comprises the following components in mass: 10 g of carpesium, 30 g of betel nut, 30 g of spina gleditsiae, 10 g of semen pharbitidis, 30 g of dark plum fruit, 10 g of mirabilite, 20 g of selfheal, 20 g of semen plantaginis, 8 g of bighead atractylodes rhizome, 8 g of codonopsis pilosula, 8 g of fructus aurantii, 8 g of platycodon grandiflorum, 8 g of medicated leaven, 20 g of hawthorn, 20 g of malt and 20 g of liquorice.

Example 2

A traditional Chinese medicine preparation for preventing and treating goat digestive tract helminthiasis comprises the following components in mass: 20 g of carpesium, 50 g of betel nut, 50 g of spina gleditsiae, 20 g of semen pharbitidis, 50 g of dark plum fruit, 20 g of mirabilite, 30 g of selfheal, 30 g of semen plantaginis, 15 g of bighead atractylodes rhizome, 15 g of codonopsis pilosula, 15 g of fructus aurantii, 15 g of platycodon grandiflorum, 15 g of medicated leaven, 30 g of hawthorn, 30 g of malt and 30 g of liquorice.

Example 3

A traditional Chinese medicine preparation for preventing and treating goat digestive tract helminthiasis comprises the following components in mass: 180 g of carpesium, 350 g of betel nut, 360 g of spina gleditsiae, 180 g of semen pharbitidis, 360 g of dark plum fruit, 120 g of mirabilite, 220 g of selfheal, 220 g of semen plantaginis, 100 g of bighead atractylodes rhizome, 100 g of radix codonopsis pilosulae, 90 g of fructus aurantii, 90 g of platycodon grandiflorum, 100 g of medicated leaven, 250 g of hawthorn, 230 g of malt and 230 g of liquorice.

Example 4

A traditional Chinese medicine preparation for preventing and treating goat digestive tract helminthiasis comprises the following components in mass: 10 g of carpesium, 40 g of betel nut, 40 g of spina gleditsiae, 10 g of semen pharbitidis, 40 g of dark plum fruit, 10 g of mirabilite, 20 g of selfheal, 20 g of semen plantaginis, 8 g of bighead atractylodes rhizome, 8 g of codonopsis pilosula, 10 g of fructus aurantii, 10 g of platycodon grandiflorum, 10 g of medicated leaven, 20 g of hawthorn, 20 g of malt and 25 g of liquorice.

Example 5

A traditional Chinese medicine preparation for preventing and treating goat digestive tract helminthiasis comprises the following components in mass: 1000 g of carpesium, 5000 g of betel nut, 5000 g of spina gleditsiae, 1200 g of semen pharbitidis, 5000 g of dark plum, 1500 g of mirabilite, 2500 g of selfheal, 2500 g of semen plantaginis, 1000 g of bighead atractylodes rhizome, 1000 g of codonopsis pilosula, 1500 g of fructus aurantii, 1500 g of platycodon grandiflorum, 1500 g of medicated leaven, 2500 g of hawthorn, 2500 g of malt and 3000 g of liquorice.

Example 6

A traditional Chinese medicine preparation for preventing and treating goat digestive tract helminthiasis comprises the following components in mass: 110 g of carpesium, 400 g of betel nut, 490 g of spina gleditsiae, 120 g of semen pharbitidis, 490 g of dark plum, 120 g of mirabilite, 220 g of selfheal, 230 g of semen plantaginis, 90 g of bighead atractylodes rhizome, 90 g of codonopsis pilosula, 120 g of fructus aurantii, 120 g of platycodon grandiflorum, 110 g of medicated leaven, 230 g of hawthorn, 230 g of malt and 260 g of liquorice.

Example 7

This example is a method of preparation of example 3, comprising the steps of:

C. weighing mirabilite according to the formula proportion, grinding into powder, mixing with fine powder A, uniformly mixing with auxiliary materials beta-cyclodextrin and maltodextrin, adding 70% ethanol, uniformly mixing, sieving with 20-30 mesh sieve, drying until the water content is less than 4%, sterilizing, and bagging to obtain the granule finished product.

Example 8

This example is a method of preparation of example 4, comprising the steps of:

C. weighing mirabilite according to the formula proportion, grinding into powder, mixing with fine powder A, uniformly mixing with auxiliary materials such as glucose and sodium carboxymethyl cellulose, adding 70% ethanol, mixing uniformly, sieving with 20-30 mesh sieve, drying until the water content is less than 4%, sterilizing, and bagging to obtain the granule finished product.

And B, concentrating the extracting solution to a concentrated solution with the relative density of 1.06 at the temperature of 50-60 ℃.

Example 9

This example is the preparation method of example 5, comprising the steps of:

A. weighing all the medicinal materials except the mirabilite according to the formula proportion, crushing, sieving with a 100-mesh sieve, soaking with 10 times of 70% ethanol overnight, heating and reflux-extracting for 3 times at 80 ℃ for 40min each time, and mixing the extracting solutions;

And B, concentrating the extracting solution to a concentrated solution with the relative density of 1.10 at the temperature of 50-60 ℃.

Example 10

This example is a method of preparation of example 6, comprising the steps of:

C. weighing Mirabilitum according to the formula proportion, grinding into powder, mixing with fine powder A, uniformly mixing with auxiliary materials such as starch, microcrystalline cellulose and sucrose, adding 70% ethanol, mixing uniformly, sieving with 20-30 mesh sieve, drying until the water content is less than 4%, sterilizing, and bagging to obtain the granule product.

And B, concentrating the extracting solution to a concentrated solution with the relative density of 1.03 at 50-60 ℃.

The auxiliary materials in the step C can be pharmaceutically acceptable pharmaceutical auxiliary materials selected from any one or more of starch, microcrystalline cellulose, sucrose, aspartame, dextrin, lactose, powdered sugar, glucose, sodium chloride, sodium carboxymethyl cellulose, crosslinked polyvinylpyrrolidone, magnesium stearate, micro powder silica gel, vitamin C, cysteine, citric acid and sodium sulfite.

Animal test

About 20kg of adult goats infected with digestive tract worms (nematodes and fasciola) are selected, and the goats are divided into 8 groups, 10 goats are fed in each group in a limiting column, the basic daily ration is the same, and the feeding management level is consistent. Groups 1-6 are granules of examples 1-6, warm water is uniformly mixed in daily ration to enable goats to self-eat on an empty stomach, and the goats are used for 3 days continuously once a day; the daily ration of the blank group is not added with any insect repellent; the control group ration was added with the chemical anthelmintic sodium chlorocyaniodate and the amounts were as described by the merchant.

Before the start of the experiment, fresh manure was collected from 8 groups of 80 goats in different houses, the eggs of the parasites in the digestive tract were checked, the eggs were counted by Liao Dangjin method, and the eggs per gram of manure (EPG) were checked for each goat. The number of Eggs (EPG) of each goat was checked in the same manner 30 days after the test administration, and all goats were weighed before the test and 30 days after the administration. During the test, whether the animal feces are excreted or not is observed, and the drink, appetite and clinical symptoms of each goat after administration are recorded.

TABLE 1 variation of number of ova in goat feces after administration

Note that: the egg negative conversion rate=the number of sheep with egg negative conversion/(the number of sheep with egg negative conversion+the number of sheep without egg negative conversion) ×; worm egg reduction rate = (pre-dosing EPG average-post-dosing EPG average)/pre-dosing EPG average x%.

Table 2 weight change of goats 1 month after taking the medicine

As can be seen from tables 1 and 2, the insect repellent, i.e., the sodium chlorocyanioxazin, has good effect of expelling sheep parasites, but the drug effect is short in maintenance time, and the feeding condition of goats after administration is poor, and weight gain is slow; after the compound preparation is adopted by the group B, the insect repellent effect is slightly lower than that of the western medicine, namely the chlorocyanioxazin sodium, but the pesticide effect is long in maintenance time, the toxic and side effects are small, the feeding capacity of the goats after administration is obviously increased, and the compound preparation has the advantages of strengthening the spleen, invigorating the stomach and increasing weight, is a broad-spectrum efficient low-toxicity insect repellent, can be used for curing goats suffering from parasitic diseases, and can play a role in preventing after being taken 1-2 times per month at ordinary times.

The foregoing examples merely illustrate specific embodiments of the invention, which are described in greater detail and are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention.

Claims

1. The traditional Chinese medicine preparation for preventing and treating goat digestive tract helminthiasis is characterized by comprising the following raw materials in parts by weight: 10 g of carpesium, 40 g of betel nut, 40 g of spina gleditsiae, 10 g of semen pharbitidis, 40 g of dark plum fruit, 10 g of mirabilite, 20 g of selfheal, 20 g of semen plantaginis, 8 g of bighead atractylodes rhizome, 8 g of codonopsis pilosula, 10 g of fructus aurantii, 10 g of platycodon grandiflorum, 10 g of medicated leaven, 20 g of hawthorn, 20 g of malt and 25 g of liquorice.

2. The method for preparing the traditional Chinese medicine preparation for preventing and treating goat digestive tract helminthiasis according to claim 1, which is characterized by comprising the following steps:

A. weighing all the medicinal materials except the mirabilite according to the proportion of the formula, crushing, soaking the medicinal materials with 10 times of 70% ethanol overnight, heating and reflux-extracting for 3 times at 80 ℃, and combining the extracting solutions;

C. weighing Mirabilitum according to the proportion of the formula, grinding into powder, mixing with the fine powder A, mixing with pharmaceutically acceptable medicinal auxiliary materials, adding 70% ethanol, adding ethanol with the amount of 10% of the mass of the materials, and mixing uniformly to obtain the granule.

3. The method for preparing a Chinese medicinal preparation for preventing and treating goat digestive tract helminthiasis according to claim 2, wherein the step B is characterized in that the extract is concentrated to a concentrated solution with a relative density of 1.03-1.10 at 50-60 ℃.

4. The method for preparing a Chinese medicinal preparation for preventing and treating goat digestive tract helminthiasis according to claim 2, wherein the medicinal materials are crushed and sieved by a 100-mesh sieve.

5. The method for preparing the traditional Chinese medicine preparation for preventing and treating goat digestive tract helminthiasis according to claim 2, wherein the heating reflux time is 40min each time.

6. The method for preparing the traditional Chinese medicine preparation for preventing and treating goat digestive tract helminthiasis according to claim 2, wherein the pharmaceutically acceptable pharmaceutic adjuvant is selected from any one or more of starch, microcrystalline cellulose, sucrose, aspartame, dextrin, lactose, powdered sugar, glucose, sodium chloride, sodium carboxymethyl cellulose, crosslinked polyvinylpyrrolidone, magnesium stearate, micro powder silica gel, vitamin C, cysteine, citric acid and sodium sulfite.