CN115053981A - Method for improving quality of heated non-combustible cigarettes by pretreating tobacco leaves with biological enzyme - Google Patents
Method for improving quality of heated non-combustible cigarettes by pretreating tobacco leaves with biological enzyme Download PDFInfo
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- CN115053981A CN115053981A CN202210628008.7A CN202210628008A CN115053981A CN 115053981 A CN115053981 A CN 115053981A CN 202210628008 A CN202210628008 A CN 202210628008A CN 115053981 A CN115053981 A CN 115053981A
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/20—Biochemical treatment
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/10—Roasting or cooling tobacco
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B5/00—Stripping tobacco; Treatment of stems or ribs
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B9/00—Control of the moisture content of tobacco products, e.g. cigars, cigarettes, pipe tobacco
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- Manufacture Of Tobacco Products (AREA)
Abstract
A method for improving the quality of a cigarette which is not combusted by heating by using biological enzyme to pretreat tobacco leaves comprises the following steps: (1) raw material treatment: removing tobacco stems in the leaves of the cured tobacco after redrying, and leaving the leaves; (2) preparing an enzyme solution: preparing alpha-amylase or cellulase into an enzyme solution; (3) enzyme pretreatment: spraying 30-50mL of enzyme solution on the surface of tobacco leaves per 100g of tobacco leaves, and pretreating the tobacco leaves sprayed with the enzyme solution in an incubator at 45-55 ℃ for 3-5h, wherein the humidity is 60-80%; (4) pretreating tobacco leaves, fermenting and alcoholizing: putting the tobacco leaves pretreated by the enzyme into an incubator at 35-40 ℃ for fermenting and alcoholizing for 6-8d to obtain tobacco leaves pretreated by the biological enzyme, fermented and alcoholized, wherein the humidity is 60-80%; (5) preparing cigarettes: the tobacco leaves after being pretreated by the biological enzyme, fermented and alcoholized are processed according to the industrial standard to prepare the cigarette which is not combusted by heating. The invention has wide significance for retaining the aroma substances of the flue-cured tobacco leaves, reducing the irritation of the tobacco leaves, improving the quality of cigarettes, shortening the fermentation period and saving the economic cost.
Description
Technical Field
The invention belongs to the field of tobacco, and particularly relates to a pretreatment method for enzymolysis and fermentation.
Background
After being redried, the freshly harvested tobacco leaves cannot be directly used for making cigarettes due to high content of harmful substances, heavy miscellaneous gas, rough smoke and lingering aftertaste. The quality of tobacco leaves is affected by the existence of some macromolecular substances, such as starch, cellulose and the like.
In the prior art, the non-fermented alcoholized flue-cured tobacco leaves also have the conditions of high content of harmful substances, strong irritation, heavy miscellaneous gas and poor aroma quality. The natural alcoholization of the tobacco needs 1 to 3 years, the aroma is full, the irritation is weak, but the occupied area of a factory and a warehouse is large, and the economic cost is high; the artificial fermentation ensures that the flue-cured tobacco leaves are fermented under certain temperature and humidity, can shorten the fermentation time, and has poorer tobacco leaf quality, aroma quality and the like than natural fermentation. At present, the fermentation period of tobacco leaves is shortened and the quality of the tobacco leaves is improved by using a biological technology, the existing technical workers add a complex enzyme preparation into the tobacco after alcoholization for one year for fermentation for 10-60 days, so that enzymolysis and fermentation are carried out simultaneously, the quality of the cigar tobacco leaves is improved, but the problem of long fermentation period is not well solved, the conditions of enzyme treatment and fermentation alcoholization are consistent, and the fermentation conditions are strict. Meanwhile, after the tobacco is treated by the enzyme, the tobacco is subjected to enzyme deactivation treatment for 10-60min at 80-100 ℃, so that the loss of aroma substances of the tobacco leaves is large at high temperature, the integral sense of a product of the tobacco is influenced by a consumer, the energy consumption of a factory is increased, and the economic cost is increased.
Disclosure of Invention
In order to overcome the defects of long fermentation period, high quality loss of high-temperature enzyme deactivation, high cost and the like in the prior art, the invention provides a method for improving the quality of heated non-combustible cigarettes by using enzyme pretreated tobacco leaves.
The technical scheme of the invention is as follows:
a method for improving the quality of a cigarette which is not combusted by heating through tobacco leaves pretreated by biological enzyme is characterized by comprising the following steps:
(1) raw material treatment: removing tobacco stems in the leaves of the cured tobacco after redrying, and leaving the leaves;
(2) preparing an enzyme solution: preparing alpha-amylase or cellulase into an enzyme solution;
(3) enzyme pretreatment: spraying the enzyme solution on the surface of tobacco leaves, and pretreating the tobacco leaves sprayed with the enzyme solution in an incubator at 45-55 ℃ for 3-5h, wherein the humidity is 60-80%;
(4) pretreating tobacco leaves, fermenting and alcoholizing: putting the tobacco leaves pretreated by the enzyme into an incubator at 35-40 ℃ for fermenting and alcoholizing for 6-8d to obtain tobacco leaves pretreated by the biological enzyme, fermented and alcoholized, wherein the humidity is 60-80%;
(5) preparing cigarettes: the tobacco leaves after being pretreated by the biological enzyme, fermented and alcoholized are processed according to the industrial standard to prepare the heating non-combustible cigarette.
Preferably, in the step (2), 30-50mL of enzyme solution is sprayed on the surface of the tobacco leaves per 100g of tobacco leaves.
Preferably, in the step (3), the tobacco leaves sprayed with the enzyme solution are filled into plastic sealing bags with small holes, and are placed in an incubator at 50 ℃ for pretreatment for 4 hours, wherein the humidity is 60-80%.
Further preferably, a plurality of plastic sealing bags are arranged, each plastic sealing bag is filled with tobacco leaves with equal quantity, treated under equal conditions and synchronously placed in the incubator.
Preferably, in the step (4), the tobacco leaves after being pretreated by the enzyme for 4 hours are put into an incubator at 37 ℃ for fermentation and alcoholization for 7 d.
Preferably, the redried flue-cured tobacco is redried Yunnan tobacco KRK 26.
Preferably, in step (2), 20U/g α -amylase is prepared using a citrate-citrate buffer at pH 7.0.
Preferably, in step (2), 10FPU/g of cellulase is prepared using a citric acid-sodium citrate buffer at pH 4.8.
The beneficial technical effects of the invention are as follows:
the invention provides an enzymolysis and fermentation method which uses a biological enzyme preparation to perform solid-state treatment on tobacco leaves and then performs fermentation, so that the quality of the cigarette products which are not combusted after being heated is obviously improved, the fragrance and aroma amount and the harmony are improved, and the fermentation time can be greatly shortened. The enzyme preparation is utilized to pretreat the tobacco leaves, certain tobacco leaf components which are not beneficial to the smoking quality can be decomposed or converted into aroma components of the tobacco leaves under the condition of artificial fermentation, and chemical components in the tobacco leaves are coordinated, so that the internal quality of the tobacco leaves is improved, the period of tobacco leaf fermentation and alcoholization can be shortened, the economic benefit is increased, the content of harmful substances is reduced, and the tobacco leaves are prevented from mildewing. The tobacco leaves treated by the method have the advantages of quick response, environmental friendliness, no generation of other harmful byproducts, mild conditions, effective reduction of tobacco leaf irritation, improvement of tobacco leaf raw material quality, increase of tobacco leaf aroma substance content, and improvement of harmonicity, aroma and taste of the cigarette which is not combusted by heating.
Specifically, compared with the prior art, the biological enzyme pretreatment and fermentation alcoholization technology used in the invention has the advantages that:
(1) according to the invention, the dry-process pretreatment is carried out on the tobacco leaf raw material by using the biological enzyme preparation, so that the chemical components of the treated tobacco leaf are changed, the reducing sugar content of the tobacco leaf is increased, and the contents of starch, cellulose, nicotine and the like in the tobacco leaf are changed.
(2) The invention uses tobacco leaves pretreated by biological enzyme to carry out fermentation alcoholization, adjusts chemical components in the tobacco leaf fermentation process and improves the tobacco leaf quality.
(3) The invention uses amylase to pretreat the tobacco leaves for fermentation and alcoholization, so that the content of the new phytodiene in the volatile aroma substances of the tobacco leaves is increased by 16.48 percent compared with that of the control.
(4) The invention uses the tobacco leaves after the biological enzyme pretreatment and the fermentation alcoholization to process and prepare the heating non-combustion cigarette, so that the total smoking score of the cigarette is obviously improved, and the quality is obviously improved. The cigarette has good penetrability, obvious roasting fragrance, tactile sensation of fragrance, smooth smoke, good comfort, less miscellaneous gas and less irritation.
(5) The method can directly carry out in-situ fermentation for 7d after the pretreatment by using the biological enzyme, can save the space of a factory, does not carry out high-temperature enzyme activation after the pretreatment by using the enzyme, and can completely retain volatile aroma substances of the tobacco leaves.
In a word, the invention researches and improves a new biological enzyme pretreatment and alcoholization technology, the enzyme treatment and fermentation alcoholization are separated, the biological enzyme preparation pretreats the flue-cured tobacco leaves under the optimal condition, the function of the biological enzyme preparation is fully exerted, and the pretreated tobacco leaves are directly placed under the proper condition without inactivation treatment for further fermentation alcoholization treatment, thereby having wide significance for retaining the aroma substances of the flue-cured tobacco leaves, reducing the irritation of the tobacco leaves, improving the quality of cigarettes, shortening the fermentation period and saving the economic cost.
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FIG. 1 is a flow chart of the fermentation and alcoholization process of tobacco leaves pretreated by amylase or cellulase.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Comparative example distilled water treatment tobacco leaf fermentation alcoholization.
(1) And selecting the re-cured Yunnan tobacco leaf KRK26, and removing tobacco stems in the leaves of the re-cured Yunnan tobacco leaf, and leaving the leaves.
(2) Treating tobacco leaves with distilled water: weighing 500g of tobacco leaves after stem removal, spraying 40mL of distilled water (spraying amount: 0.4mL/g) on the surface of the tobacco leaves by using a rotary spray bottle per 100g of tobacco leaves, filling the tobacco leaves sprayed with the distilled water into a plastic sealing bag with small holes, and placing the plastic sealing bag in an incubator at 50 ℃ for treatment for 4 hours, wherein the humidity is 60-80%.
(3) Fermenting and alcoholizing tobacco leaves: and (3) putting the tobacco leaves treated by the distilled water for 4 hours into an incubator at 37 ℃ for fermentation and alcoholization for 7 days, wherein the humidity is 60-80%, and obtaining the treated and fermented tobacco leaves.
(4) Preparing cigarettes: the tobacco leaves after the biological enzyme pretreatment and fermentation are processed according to the industrial standard to prepare the cigarette which is not combusted by heating.
Sensory evaluation: and (4) performing smoking evaluation on the prepared finished cigarette product which is not burnt by heating by a smoking expert. Smoking evaluation indexes 6 indexes of the smoke amount, aroma, fragrance, strength, harmony, irritation and taste of the cigarette bomb are evaluated and scored according to a novel cigarette sensory evaluation method (Q/YNZY.J04.022-2015) of cigarette enterprise standards in Yunnan (see comparative example data in each example list for details).
Example 1 fermentative alcoholization of alpha-Amylase pretreated tobacco leaves
(1) Treating tobacco leaf raw materials: and selecting the re-cured Yunnan tobacco leaf KRK26, and removing tobacco stems in the leaves of the re-cured Yunnan tobacco leaf, and leaving the leaves.
(2) Preparing biological enzyme: 500g of the tobacco leaves after stem removal are weighed and 20U/g of alpha-amylase is prepared by using a citric acid-sodium citrate buffer solution with the pH value of 7.0. The alpha-amylase used was the meso-amylase BAN 480L, purchased from Novozymes, with an enzyme activity of 3042U/mL.
(3) Pretreating tobacco leaves by biological enzyme: spraying 40mL of enzyme solution (spraying amount is 0.4mL/g) on the surface of each 100g of tobacco leaves by using a rotary spray bottle, putting the tobacco leaves sprayed with the enzyme solution into a plurality of plastic sealing bags with small holes, pre-treating for 4 hours in an incubator at 50 ℃, wherein the humidity is 60-80%, each plastic sealing bag is filled with uniform and equivalent tobacco leaves, treating under the same conditions, and synchronously putting the plastic sealing bags into the incubator.
(4) Pretreating tobacco leaves, fermenting and alcoholizing: and (3) putting the tobacco leaves pretreated by the biological enzyme for 4 hours into an incubator at 37 ℃ for fermentation and alcoholization for 7 days, wherein the humidity is 60-80%, so as to obtain the fermented and alcoholized tobacco leaves pretreated by the biological enzyme.
(5) Preparing cigarettes: the tobacco leaves after the biological enzyme pretreatment and fermentation are processed and prepared into the cigarette which can not be burnt by heating according to the industrial standard.
Sensory evaluation: and (4) performing smoking evaluation on the prepared finished cigarette product which is not combusted by heating by a smoking evaluation expert. The smoking evaluation indexes evaluate and score 6 indexes of the smoke amount, aroma, strength, harmony, irritation and taste of the cigarette bomb according to a novel cigarette sensory evaluation method (Q/YNZY.J04.022-2015) of cigarette enterprise standards in Yunnan.
TABLE 1 sensory quality evaluation table for amylase pretreatment fermented cigarette
It can be seen from table 1 that the total smoking score of the non-combustible cigarette obtained by the method of example 1 is 86.5 (total score 100), and the quality of the non-combustible cigarette is improved significantly, so that the cigarette has good permeability, obvious roasting aroma, texture of aroma, soft smoke, good comfort, less offensive odor and less irritation. Compared with a comparative example, the comprehensive total smoking score is improved, the fragrance and the aroma are improved, the strength is increased, the harmonicity is enhanced, and the mouthfeel is improved.
TABLE 2 chemical composition table of tobacco leaves after amylase pretreatment
As can be seen from Table 2, the chemical components of the tobacco leaves are significantly changed after the tobacco leaves are pretreated by the amylase, and compared with the tobacco leaves sprayed with the same amount of distilled water, the contents of total sugar and reducing sugar treated by the amylase are respectively increased by 2.87% and 45.56%, the content of starch is reduced by 30.36%, and the content of nicotine is reduced by 18.48%. The amylase can change the chemical components of the tobacco leaves, thereby improving the quality of the tobacco leaves.
TABLE 3 table of volatile aroma component content of tobacco leaves after 7 days of amylase pretreatment and fermentation
As can be seen from Table 3, the total amount of volatile aroma components in the tobacco leaves after 7 days of the amylase pretreatment fermentation is 504.08 mug/g, wherein the olefin content accounts for 81.57%, wherein the neophytadiene accounts for 78.37%, and the content is increased by 16.48% compared with the control. The new phytodiene can increase the fragrance of the tobacco leaves and reduce the stimulation to the throat, and is one of the key components which can make the greatest contribution to the fragrance and the flavor of the tobacco leaves. The results show that after the tobacco leaves pretreated by enzyme are fermented, the volatile aroma components of the tobacco leaves can be increased, so that the aroma and flavor substances of the tobacco leaves are increased, and the sensory evaluation smoking quality of the heated non-burning cigarette products is improved.
Example 2 cellulase pretreated tobacco leaves were fermented and alcoholized.
(1) Treating tobacco leaf raw materials: and (3) selecting the Yunnan tobacco leaf KRK26 after redrying, and removing tobacco stems in leaves of the redried tobacco leaves to leave the leaves.
(2) Preparing biological enzyme: 500g of the tobacco leaves after stem removal were weighed and 10FPU/g of cellulase was prepared using a citric acid-sodium citrate buffer solution with a pH of 4.8. The cellulase used was SAE0020, purchased from Sigma, and the enzyme activity was 127.59 FPU/mL.
(3) Pretreating tobacco leaves by biological enzyme: spraying 40mL of enzyme solution (spraying amount is 0.4mL/g) on the surface of each 100g of tobacco leaves by using a rotary spray bottle, filling the tobacco leaves sprayed with the enzyme solution into plastic sealing bags with small holes, placing the plastic sealing bags in an incubator at 50 ℃ for enzyme treatment for 4 hours, wherein the humidity is 60-80%, uniformly and equivalently filling the plastic sealing bags with the tobacco leaves, treating under the same conditions, and synchronously placing the plastic sealing bags in the incubator.
(4) Pretreating tobacco leaves, fermenting and alcoholizing: and (3) putting the tobacco leaves pretreated by the biological enzyme for 4 hours into an incubator at 37 ℃ for fermentation and alcoholization for 7 days, wherein the humidity is 60-80%, so as to obtain the fermented and alcoholized tobacco leaves pretreated by the biological enzyme.
(5) Preparing cigarette rods: the tobacco leaves after the biological enzyme pretreatment and fermentation are processed according to the industrial standard to prepare the cigarette which is not combusted by heating.
Sensory evaluation: and (4) performing smoking evaluation on the prepared finished cigarette product which is not combusted by heating by a smoking evaluation expert. The smoking evaluation indexes evaluate and score 6 indexes of the smoke amount, aroma, strength, harmony, irritation and taste of the cigarette bomb according to a novel cigarette sensory evaluation method (Q/YNZY.J04.022-2015) of cigarette enterprise standards in Yunnan.
TABLE 4 evaluation table for sensory quality of cigarettes treated and fermented by cellulase
It can be seen from table 4 that the total smoking score of the non-combustible heated cigarette obtained by the method is 83.5 (100), the quality is obviously improved, and the cigarette has good sweet feeling, slight fluid production feeling, slightly scorched, thin aroma, and unclean residue. Compared with the contrast, the total comprehensive smoking score is improved, the fragrance and the aroma are improved, the strength is increased, and the mouthfeel is improved.
TABLE 5 chemical composition table of cellulase-pretreated tobacco leaves
From table 5, it can be seen that the chemical components of the tobacco leaves after the cellulose enzyme pretreatment are changed, and compared with the tobacco leaves sprayed with the same amount of distilled water, the content of total sugar and reducing sugar treated by the cellulose enzyme is respectively increased by 2.87% and 53.94%, the content of cellulose is reduced by 15.96%, and the content of nicotine is reduced by 15.08%. The cellulase is proved to change the chemical components of the tobacco leaves, so that the chemical components in the tobacco leaves are in a proper range, and the quality of the tobacco leaves is improved.
TABLE 6 table of volatile aroma component content of tobacco leaves after 7 days of cellulase pretreatment and fermentation
As can be seen from Table 6, the total amount of volatile aroma components in the tobacco leaves during the 7d fermentation of the cellulase pretreatment is 264.59 mug/g, and the total amount of the aroma components is lower than that of the tobacco leaves treated by the amylase and lower than that of the tobacco leaves treated by the control group. The cellulase pretreatment of the tobacco leaves destroys the coordination of aroma substances in the tobacco leaves, so that the total amount of volatile aroma components is reduced. But the content of the volatile nicotine pretreated by the cellulase is 1.95 times of that of the control, and the suitable nicotine is one of the strength and the mouthfeel source of the cigarette, so the sensory score of the treated cigarette is higher than that of the control.
The foregoing is only an embodiment of the present invention, and it will be apparent to those skilled in the art that the present invention may be modified in various aspects and modifications of the foregoing embodiments, or may be modified in equivalents of some of the features; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (8)
1. A method for improving the quality of a cigarette which is not combusted by heating through tobacco leaves pretreated by biological enzyme is characterized by comprising the following steps:
(1) raw material treatment: removing tobacco stems in the leaves of the cured tobacco after redrying, and leaving the leaves;
(2) preparing an enzyme solution: preparing alpha-amylase or cellulase into an enzyme solution;
(3) enzyme pretreatment: spraying the enzyme solution on the surface of tobacco leaves, and pretreating the tobacco leaves sprayed with the enzyme solution in an incubator at 45-55 ℃ for 3-5h, wherein the humidity is 60-80%;
(4) pretreating tobacco leaves, fermenting and alcoholizing: putting the tobacco leaves pretreated by the enzyme into an incubator at 35-40 ℃ for fermenting and alcoholizing for 6-8d to obtain tobacco leaves pretreated by the biological enzyme, fermented and alcoholized, wherein the humidity is 60-80%;
(5) preparing cigarettes: the tobacco leaves after being pretreated by the biological enzyme, fermented and alcoholized are processed according to the industrial standard to prepare the cigarette which is not combusted by heating.
2. The method according to claim 1, wherein in the step (2), 30-50mL of the enzyme solution is sprayed on the surface of the tobacco leaves per 100g of the tobacco leaves.
3. The method according to claim 1, wherein in the step (3), the tobacco leaves sprayed with the enzyme solution are filled into plastic sealing bags with small holes, and are placed in an incubator at 50 ℃ for pretreatment for 4 hours, wherein the humidity is 60-80%.
4. A method according to claim 3, wherein a plurality of plastic sealed bags are provided, each plastic sealed bag containing a uniform quantity of tobacco leaves, treated under the same conditions, and synchronously placed in the incubator.
5. The method according to claim 1, wherein in the step (4), the tobacco leaves after 4 hours of enzyme pretreatment are put into a 37 ℃ incubator for fermentation alcoholization for 7 d.
6. The method according to claim 1, characterized in that the redried flue-cured tobacco is redried Yunnan tobacco leaf KRK 26.
7. The method according to claim 1, wherein in step (2), 20U/g of α -amylase is prepared in a pH 7.0 citric acid-sodium citrate buffer.
8. The method according to claim 1, wherein in step (2), 10FPU/g of cellulase is prepared using a citric acid-sodium citrate buffer at pH 4.8.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1593268A (en) * | 2004-06-25 | 2005-03-16 | 云南瑞升科技有限公司 | Immobilized biological additive and its preparation method and uses |
| CN102181414A (en) * | 2011-02-23 | 2011-09-14 | 红云红河烟草(集团)有限责任公司 | Complex enzyme additive for tobacco and preparation method and application thereof |
| CN111038909A (en) * | 2019-12-06 | 2020-04-21 | 红云红河烟草(集团)有限责任公司 | Raw material preparation process based on heating non-combustion cigarette |
| CN111972694A (en) * | 2020-09-17 | 2020-11-24 | 内蒙古昆明卷烟有限责任公司 | Processing method of threshing and redrying tobacco leaves |
| CN112586799A (en) * | 2020-12-28 | 2021-04-02 | 河南中烟工业有限责任公司 | Henan tobacco leaf rapid alcoholization control method |
-
2022
- 2022-06-06 CN CN202210628008.7A patent/CN115053981A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1593268A (en) * | 2004-06-25 | 2005-03-16 | 云南瑞升科技有限公司 | Immobilized biological additive and its preparation method and uses |
| CN102181414A (en) * | 2011-02-23 | 2011-09-14 | 红云红河烟草(集团)有限责任公司 | Complex enzyme additive for tobacco and preparation method and application thereof |
| CN111038909A (en) * | 2019-12-06 | 2020-04-21 | 红云红河烟草(集团)有限责任公司 | Raw material preparation process based on heating non-combustion cigarette |
| CN111972694A (en) * | 2020-09-17 | 2020-11-24 | 内蒙古昆明卷烟有限责任公司 | Processing method of threshing and redrying tobacco leaves |
| CN112586799A (en) * | 2020-12-28 | 2021-04-02 | 河南中烟工业有限责任公司 | Henan tobacco leaf rapid alcoholization control method |
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