CN106387982A - Method for preparing and reproducing tobacco leaves with microorganisms - Google Patents
Method for preparing and reproducing tobacco leaves with microorganisms Download PDFInfo
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- CN106387982A CN106387982A CN201611178971.0A CN201611178971A CN106387982A CN 106387982 A CN106387982 A CN 106387982A CN 201611178971 A CN201611178971 A CN 201611178971A CN 106387982 A CN106387982 A CN 106387982A
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- tobacco
- microorganism
- reconstituted tobacco
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- extraction
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/14—Forming reconstituted tobacco products, e.g. wrapper materials, sheets, imitation leaves, rods, cakes; Forms of such products
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- Manufacture Of Tobacco Products (AREA)
Abstract
The invention discloses a method for preparing and reproducing tobacco leaves with microorganisms and belongs to the technical field of tobacco. The method comprises the steps of adding bacillus cereus into soaked tobacco raw materials to degrade protein in the soaked tobacco raw materials, and generating precursor-aroma substances by participating in a Maillard reaction. According to the method for preparing and reproducing tobacco leaves with microorganisms, during the process of extracting the tobacco raw materials, the bacillus cereus is added, the protein in the tobacco raw materials can be degraded to the utmost extent to generate polypeptide or free amino acid, meanwhile, soluble reducing sugar in the tobacco raw materials during the extracting process is dissolved in the extracted liquor, which gives rise to the Maillard reaction between the degraded product and the soluble reducing sugar, thus the precursor-aroma substances with heterocyclic compounds are generated. These precursor-aroma substances participates in complex chemical reactions to generate aromas through thermal cracking when cigarettes burns, thus good harmony and compatibility are generated between the reproduced tobacco leaves and the cigarettes and taste quality of the cigarettes is improved.
Description
Technical field
The present invention relates to a kind of method preparing reconstituted tobacco, prepare reconstituted tobacco particularly to a kind of using microorganism
Method, belongs to technical field of tobacco.
Background technology
During China's production of cigarettes, a large amount of tobacco wastes can be produced.For these dead meals, countries in the world are main
By the method for reconstituted tobacco, again dead meal is utilized, the production method of reconstituted tobacco mainly has roll-in method, thick slurry method
And paper process.China mainly adopts the tobacco leaf dead meal in paper process processing production process at present.Paper process is by tobacco system
Make some tobacco wastes in the redrying and cigarette making process in link, leftover pieces, such as offal, offal, cigarette label, cigarette rod and
The tobacco materials such as part discarded tobacco leaf, make the thin slice having with tobacco same performance and fill reconstituted product, fill as cigarette former
Material.Tobacco can also be added to according to a certain percentage to roll in link.On the one hand the consumption of high-grade tobacco leaf can be reduced, improve cigarette
The utilization rate of grassland material, reduces unit consumption of tobacco leaf, effectively reduces cigarette cost.On the other hand can by artificial process so that
Harmful substance in some tobaccos is degraded, and improves the physical characteristic of tobacco leaf, chemical composition, aesthetic quality, and reaches fall
Burnt harm reduction, the purpose of upgrading flavouring.
At present, it has been directed to and reduced the relevant report that macromolecular substances in reconstituted tobacoo improve reconstituted tobacoo aesthetic quality.
Such as patent CN201510041569 discloses the preparation method of reconstituted tobacoo, and it has been changed in offal, offal in leaching process
In solution, water is replaced by alcoholic solution, so that protein in tobacco leaf, pectin content is reduced, the excitant of reconstituted tobacoo mitigates,
Miscellaneous gas reduces, and suction aesthetic quality make moderate progress, and fragrance pleasant impression is significantly increased.For example patent CN201510113008 discloses
A kind of method of lifting sensory quality of reconstituted tobacco leaves, is that the weak base potassium citrate aqueous solution of suitable tobacco variety is sprayed at tobacco
So that the aesthetic quality of reconstituted tobacoo is lifted well on the pipe tobacco of sheet support, and operating condition is simple.
Maillard reaction is a kind of universal non-enzymatic browning phenomenon, is applied in the production of food flavor spices, state
Outer research is relatively more, and studies in China is less, and this technology has extraordinary application in meat flavoring and tobacco essence.U.S. rad is anti-
Answer application in essence field for the technology to break the category of traditional flavor deployment and production technology, be a kind of brand-new essence
Fragrance applications technology.At present, Maillard reaction passes through microbial process in tobacco leaf production especially reconstituted tobacco in leaching stages
Promote occur Maillard reaction thus produce some aromatic substances come to improve the organoleptic quality of reconstituted tobacco application study also not
Appear in the newspapers.
Content of the invention
In order to solve the above problems, it is an object of the invention to provide a kind of method preparing reconstituted tobacco, the method is simple
Easy, easy to operate, safe and effective, the aesthetic quality of reconstituted tobacco can be effectively improved.
To achieve these goals, the technical solution adopted in the present invention is：
A kind of method preparing reconstituted tobacco using microorganism, Bacillus cercus is added to the tobacco material soaking
In, protein in degrading tobacco raw material, participate in Maillard reaction, produce latent Studies of The Aromatic Substances.
The method that described utilization microorganism prepares reconstituted tobacco, comprises the following steps：
(1) tobacco material and water are added in fermentation tank, add Bacillus cercus bacterium solution, extraction, while extraction
There is Maillard reaction, extraction carries out after terminating separating, and obtains leaching liquor and soluble thing；
(2) leaching liquor is carried out rotary evaporation in vacuo and be concentrated to give concentrate, soluble thing is carried out paper technology and manufactures paper with pulp
Become substrate；
(3) concentrate is coated on substrate, dries and reconstituted tobacco is obtained.
In step (1), the mass ratio of tobacco material and water is 1:5-6.
In step (1), the inoculum concentration of Bacillus cercus bacterium solution is 6-10%, and the OD value of bacterium solution is 1.0.
In step (1), the joining day of Bacillus cercus bacterium solution is tobacco material and water adds after 20min in fermentation tank.
In step (1), the condition of extraction is：Temperature 35-50 DEG C, time 2-3h, mixing speed 150-200rpm, dissolved oxygen amount
20%.
In step (2), the Baume degrees of concentrate is 25 ° of B é.
Described tobacco material is mass ratio 5:3:The mixture of 2 offal, offal and rudimentary tobacco leaf.
Beneficial effects of the present invention：
1st, the present invention adds wax printing fabric in tobacco material leaching process, can to the full extent degrading tobacco former
Protein in material, generates polypeptide or free amino acid, meanwhile, using the solubility reduction in tobacco material in leaching process
Sugar is dissolved in leaching liquor, makes the catabolite of protein, with soluble reducing sugars, Maillard reaction occur, thus produce have miscellaneous
The latent Studies of The Aromatic Substances of cycle compound.These latent Studies of The Aromatic Substances pass through thermal cracking in cigarette burning, participate in complicated chemical reaction, produce
Fragrance, so that producing good harmony and compatibility between reconstituted tobacco and raw material of cigarette, improves the jealous quality of cigarette.
2 it is demonstrated experimentally that the present invention adopts in the reconstituted tobacco of Bacillus cercus preparation, identifies 128 kinds of neutral perfume altogether
Gas composition, considerably beyond 83 kinds degraded using neutral proteinase, and 60 kinds of blank, this fully proves using wax-like bud
Spore bacillus prepares the aroma quality that reconstituted tobacco can significantly improve cigarette.
3rd, the present invention adopts microorganism to participate in extraction, need not add natural flavor spices, safe and pollution-free, simple to operate,
It is applied to industrialized production, there is good Social and economic benef@, be that the one of reconstituted tobacco preparation method innovates greatly.
Brief description
Fig. 1 adds the neutral volatile ingredient testing result of the reconstituted tobacco of Bacillus cercus preparation for the present invention.
Fig. 2 is the neutral volatile ingredient testing result of the reconstituted tobacco that reference examples 1 add neutral proteinase preparation.
Fig. 3 is the neutral volatile ingredient testing result of the reconstituted tobacco not adding any material preparation.
Specific embodiment
To further illustrate content of the invention below in conjunction with specific embodiment, in the case of there is no specified otherwise, to be adopted
Operational means, verification method etc. are all the routine techniques means of those skilled in the art, and the present invention simply highlights technical scheme
Innovative point, therefore prior art here is not described further.
Bacillus cercus used by the present invention is ATCC 13061.
The preparation method of Bacillus cercus bacterium solution：Take out the bacterial classification preserving in -80 DEG C of refrigerators, first carry out actication of culture,
Inoculum concentration is the 1 ‰ of nutrient solution, carries out inoculated and cultured after activation, prepares bacterium solution.It is LB culture medium using nutrient solution：Peptone
1%, dusty yeast 0.5%, NaCl 1g, water 100ml, pH=7.
Embodiment 1
A kind of method preparing reconstituted tobacco using microorganism, comprises the following steps：
(1) tobacco material and water is 1 according to mass ratio:6 ratio adds in fermentation tank, after extraction 20min, according still further to
6% inoculum concentration (tobacco material+water) adds Bacillus cercus bacterium solution, and the OD value of bacterium solution is 1.0, and extraction, while extraction
There is Maillard reaction, extraction carries out after terminating separating, and obtains leaching liquor and soluble thing；
Extraction condition be：Temperature 50 C, time 2h, mixing speed 150rpm, dissolved oxygen amount 20%；
Tobacco material is mass ratio 5:3:The mixture of 2 offal, offal and rudimentary tobacco leaf；
(2) leaching liquor is carried out rotary evaporation in vacuo and be concentrated to give concentrate, the Baume degrees of concentrate is 25 ° of B é；Will not
DDGS carries out paper technology and manufactures paper with pulp into substrate；
(3) concentrate is coated on substrate, dries and reconstituted tobacco is obtained.
Embodiment 2
A kind of method preparing reconstituted tobacco using microorganism, comprises the following steps：
(1) tobacco material and water is 1 according to mass ratio:6 ratio adds in fermentation tank, after extraction 20min, according still further to
10% inoculum concentration adds Bacillus cercus bacterium solution, and the OD value of bacterium solution is 1.0, extraction, occurs Mei Lade anti-while extraction
Should, extraction carries out after terminating separating, and obtains leaching liquor and soluble thing；
Extraction condition be：40 DEG C of temperature, time 2h, mixing speed 150rpm, dissolved oxygen amount 20%；
Tobacco material is mass ratio 5:3:The mixture of 2 offal, offal and rudimentary tobacco leaf；
(2) leaching liquor is carried out rotary evaporation in vacuo and be concentrated to give concentrate, the Baume degrees of concentrate is 25 ° of B é；Will not
DDGS carries out paper technology and manufactures paper with pulp into substrate；
(3) concentrate is coated on substrate, dries and reconstituted tobacco is obtained.
Embodiment 3
A kind of method preparing reconstituted tobacco using microorganism, comprises the following steps：
(1) tobacco material and water is 1 according to mass ratio:6 ratio adds in fermentation tank, after extraction 20min, according still further to
8% inoculum concentration adds Bacillus cercus bacterium solution, and the OD value of bacterium solution is 1.0, extraction, occurs Mei Lade anti-while extraction
Should, extraction carries out after terminating separating, and obtains leaching liquor and soluble thing；
Extraction condition be：35 DEG C of temperature, time 3h, mixing speed 150rpm, dissolved oxygen amount 20%；
Tobacco material is mass ratio 5:3:The mixture of 2 offal, offal and rudimentary tobacco leaf；
(2) leaching liquor is carried out rotary evaporation in vacuo and be concentrated to give concentrate, the Baume degrees of concentrate is 25 ° of B é；Will not
DDGS carries out paper technology and manufactures paper with pulp into substrate；
(3) concentrate is coated on substrate, dries and reconstituted tobacco is obtained.
Reference examples 1
A kind of method preparing reconstituted tobacco using microorganism, comprises the following steps：
(1) tobacco material and water is 1 according to mass ratio:6 ratio adds in fermentation tank, after extraction 20min, adds
Neutral proteinase (purchased from the summer contain (Beijing) biotechnology development corporation, Ltd.), addition be 2 ‰, continue extraction, extraction same
Shi Fasheng Maillard reaction, extraction carries out after terminating separating, and obtains leaching liquor and soluble thing；
Extraction condition be：35 DEG C of temperature, time 3h, mixing speed 150rpm；
Tobacco material is mass ratio 5:3:The mixture of 2 offal, offal and rudimentary tobacco leaf；
(2) leaching liquor is carried out rotary evaporation in vacuo and be concentrated to give concentrate, the Baume degrees of concentrate is 25 ° of B é；Will not
DDGS carries out paper technology and manufactures paper with pulp into substrate；
(3) concentrate is coated on substrate, dries and reconstituted tobacco is obtained.
Reference examples 2
A kind of method preparing reconstituted tobacco using microorganism, comprises the following steps：
(1) tobacco material and water is 1 according to mass ratio:6 ratio adds in fermentation tank, and extraction is sent out while extraction
Raw Maillard reaction, extraction carries out after terminating separating, and obtains leaching liquor and soluble thing；
Extraction condition be：35 DEG C of temperature, time 3h, mixing speed 150rpm；
Tobacco material is mass ratio 5:3:The mixture of 2 offal, offal and rudimentary tobacco leaf；
(2) leaching liquor is carried out rotary evaporation in vacuo and be concentrated to give concentrate, the Baume degrees of concentrate is 25 ° of B é；Will not
DDGS carries out paper technology and manufactures paper with pulp into substrate；
(3) concentrate is coated on substrate, dries and reconstituted tobacco is obtained.
Test example 1 Neutral Volatile Components measure analysis
It is analyzed detecting using the fragrance component that GC/MS extracts to Simultaneous distillation, concrete outcome is as follows：
The reconstituted tobacco of the present invention：Identify 128 kinds of Neutral Aroma Components altogether.Including 16 kinds of ketone, in aldehydes 9,
24 kinds of alcohols, 45 kinds of esters, 34 kinds of oxygen heterocycle class (result is shown in Fig. 1).
The reconstituted tobacco of reference examples 1：Identify 83 kinds of Neutral Aroma Components altogether.Including 16 kinds of ketone, 8 kinds of aldehydes,
13 kinds of alcohols, 26 kinds of esters, 20 kinds of oxygen heterocycle class (result is shown in Fig. 2).
The reconstituted tobacco of reference examples 2：Identify 60 kinds of Neutral Aroma Components altogether, including 12 kinds of ketone, 7 kinds of aldehydes,
11 kinds of alcohols, 18 kinds of esters, 12 kinds of oxygen heterocycle class (result is shown in Fig. 3).
Result above shows, participates in the reconstituted tobacco of fermentation extraction using microorganism of the present invention, with addition neutral proteinase
Compare with the reconstituted tobacco being added without any material, neutral volatile material is respectively increased 54.2% and 113.3%, have substantially
Flavouring effect.
Test example 2 is smoked panel test test
By reconstituted tobacco of the present invention after chopping, put into balance 48h in constant temperature and humidity incubator, make cigarette, as
Sample sets, the cigarette as a control group 1,2 that the reconstituted tobacco of reference examples 1,2 preparation is obtained, by sample sets and control group 1,2 with
Machine is numbered, and forms, by the member of smoking possessing qualification of smokeing panel test, the group of smokeing panel test and carries out sensory evaluating smoking, result see table.
Evaluation result shows：Compared with control group, the miscellaneous gas of the reconstituted tobacco of the present invention, pleasant impression make moderate progress, and tobacco is originally
Perfume (or spice) becomes apparent from.Under identical process conditions, the reconstituted tobacco that the present invention is obtained more can project this perfume of tobacco than conventional method.
It should be pointed out that for Field of Tobacco, under the premise without departing from the principles of the invention, making some deformation and improve,
These are also considered as belonging to protection scope of the present invention.
Claims (8)
1. a kind of using microorganism prepare reconstituted tobacco method it is characterised in that by Bacillus cercus add to soak
In tobacco material, protein in degrading tobacco raw material, participate in Maillard reaction, produce latent Studies of The Aromatic Substances.
2. according to claim 1 prepare the method for reconstituted tobacco it is characterised in that including following walking using microorganism
Suddenly：
(1) tobacco material and water are added in fermentation tank, add Bacillus cercus bacterium solution, extraction, occur while extraction
Maillard reaction, extraction carries out after terminating separating, and obtains leaching liquor and soluble thing；
(2) leaching liquor is carried out rotary evaporation in vacuo and be concentrated to give concentrate, soluble thing is carried out paper technology and manufactures paper with pulp into base
Piece；
(3) concentrate is coated on substrate, dries and reconstituted tobacco is obtained.
3. according to claim 2 prepare the method for reconstituted tobacco it is characterised in that cigarette in step (1) using microorganism
The mass ratio of grassland material and water is 1:5-6.
4. according to claim 2 prepare the method for reconstituted tobacco it is characterised in that wax in step (1) using microorganism
The inoculum concentration of shape bacillus bacterium solution is 6-10%, and the OD value of bacterium solution is 1.0.
5. according to claim 2 prepare the method for reconstituted tobacco it is characterised in that wax in step (1) using microorganism
After the joining day of shape bacillus bacterium solution is 20min in tobacco material and water addition fermentation tank.
6. according to claim 2 using microorganism prepare reconstituted tobacco method it is characterised in that step (1) in leaching
The condition carrying is：Temperature 35-50 DEG C, time 2-3h, mixing speed 150-200rpm, dissolved oxygen amount 20%.
7. according to claim 2 prepare the method for reconstituted tobacco using microorganism it is characterised in that dense in step (2)
The Baume degrees of contracting liquid is 25 ° of B é.
8. the utilization microorganism according to any one of claim 1-7 prepares the method for reconstituted tobacco it is characterised in that described
Tobacco material be mass ratio 5:3:The mixture of 2 offal, offal and rudimentary tobacco leaf.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106974321A (en) * | 2017-05-23 | 2017-07-25 | 安徽中烟工业有限责任公司 | A kind of cigarette for highlighting the fragrant fragrance of head mouthful and preparation method thereof |
| CN107299115A (en) * | 2017-06-24 | 2017-10-27 | 常州市雪宜物资有限公司 | A kind of preparation method of reconstituted tobacco additive |
| CN107361402A (en) * | 2017-07-11 | 2017-11-21 | 东莞波顿香料有限公司 | Recombinate reconstituted tobacoo and preparation method thereof |
| CN107625194A (en) * | 2017-08-30 | 2018-01-26 | 常州市海若纺织品有限公司 | A kind of preparation method of reconstituted tobacco |
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| CN101416778A (en) * | 2008-11-30 | 2009-04-29 | 华南理工大学 | Ferment for tobacco fermentation and use thereof |
| CN102119784A (en) * | 2011-02-23 | 2011-07-13 | 红云红河烟草(集团)有限责任公司 | Spice extractum prepared by waste tobacco leaf, preparation method thereof and application thereof |
| CN103251126A (en) * | 2013-05-14 | 2013-08-21 | 红云红河烟草(集团)有限责任公司 | Method for preparing microorganism derived spice component for improving cigarette quality |
| CN103725632A (en) * | 2013-12-17 | 2014-04-16 | 浙江中烟工业有限责任公司 | Bacillus subtilis SM separated from tobacco waste concentrated solution and application of bacillus subtilis SM in paper-making reconstituted tobaccos |
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2016
- 2016-12-19 CN CN201611178971.0A patent/CN106387982B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101416778A (en) * | 2008-11-30 | 2009-04-29 | 华南理工大学 | Ferment for tobacco fermentation and use thereof |
| CN102119784A (en) * | 2011-02-23 | 2011-07-13 | 红云红河烟草(集团)有限责任公司 | Spice extractum prepared by waste tobacco leaf, preparation method thereof and application thereof |
| CN103251126A (en) * | 2013-05-14 | 2013-08-21 | 红云红河烟草(集团)有限责任公司 | Method for preparing microorganism derived spice component for improving cigarette quality |
| CN103725632A (en) * | 2013-12-17 | 2014-04-16 | 浙江中烟工业有限责任公司 | Bacillus subtilis SM separated from tobacco waste concentrated solution and application of bacillus subtilis SM in paper-making reconstituted tobaccos |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106974321A (en) * | 2017-05-23 | 2017-07-25 | 安徽中烟工业有限责任公司 | A kind of cigarette for highlighting the fragrant fragrance of head mouthful and preparation method thereof |
| CN107299115A (en) * | 2017-06-24 | 2017-10-27 | 常州市雪宜物资有限公司 | A kind of preparation method of reconstituted tobacco additive |
| CN107299115B (en) * | 2017-06-24 | 2021-08-20 | 谢新昇 | Preparation method of reconstituted tobacco additive |
| CN107361402A (en) * | 2017-07-11 | 2017-11-21 | 东莞波顿香料有限公司 | Recombinate reconstituted tobacoo and preparation method thereof |
| CN107625194A (en) * | 2017-08-30 | 2018-01-26 | 常州市海若纺织品有限公司 | A kind of preparation method of reconstituted tobacco |
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| CN106387982B (en) | 2017-11-10 |
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