CN105054286B - Method for preparing cigar tobacco leaves by means of fermentation effect of compounded enzyme preparation - Google Patents
Method for preparing cigar tobacco leaves by means of fermentation effect of compounded enzyme preparation Download PDFInfo
- Publication number
- CN105054286B CN105054286B CN201510359765.9A CN201510359765A CN105054286B CN 105054286 B CN105054286 B CN 105054286B CN 201510359765 A CN201510359765 A CN 201510359765A CN 105054286 B CN105054286 B CN 105054286B
- Authority
- CN
- China
- Prior art keywords
- nicotiana tabacum
- cigar
- enzymic preparation
- compound enzymic
- cigar tobacco
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 151
- 235000019506 cigar Nutrition 0.000 title claims abstract description 88
- 238000002360 preparation method Methods 0.000 title claims abstract description 78
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 52
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 52
- 241000208125 Nicotiana Species 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 48
- 238000000855 fermentation Methods 0.000 title claims abstract description 32
- 230000004151 fermentation Effects 0.000 title claims abstract description 31
- 230000000694 effects Effects 0.000 title abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
- 239000000796 flavoring agent Substances 0.000 claims abstract description 18
- 235000019634 flavors Nutrition 0.000 claims abstract description 18
- 244000061176 Nicotiana tabacum Species 0.000 claims description 100
- 150000001875 compounds Chemical class 0.000 claims description 70
- 229940088598 enzyme Drugs 0.000 claims description 46
- 108091005804 Peptidases Proteins 0.000 claims description 23
- 239000004365 Protease Substances 0.000 claims description 23
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 23
- 102000004139 alpha-Amylases Human genes 0.000 claims description 23
- 108090000637 alpha-Amylases Proteins 0.000 claims description 23
- 229940024171 alpha-amylase Drugs 0.000 claims description 23
- 102000003992 Peroxidases Human genes 0.000 claims description 22
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 22
- 108010059892 Cellulase Proteins 0.000 claims description 21
- 229940106157 cellulase Drugs 0.000 claims description 21
- 230000002255 enzymatic effect Effects 0.000 claims description 17
- 239000000203 mixture Substances 0.000 claims description 13
- 238000002386 leaching Methods 0.000 claims description 11
- 239000007921 spray Substances 0.000 claims description 10
- 238000001514 detection method Methods 0.000 claims description 9
- 239000008367 deionised water Substances 0.000 claims description 7
- 229910021641 deionized water Inorganic materials 0.000 claims description 7
- 239000000835 fiber Substances 0.000 claims description 2
- 238000009472 formulation Methods 0.000 claims description 2
- 239000007864 aqueous solution Substances 0.000 abstract description 25
- 239000000779 smoke Substances 0.000 abstract description 18
- 235000019658 bitter taste Nutrition 0.000 abstract description 15
- 235000019640 taste Nutrition 0.000 abstract description 13
- 239000002994 raw material Substances 0.000 abstract description 6
- 238000012545 processing Methods 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 3
- 235000019504 cigarettes Nutrition 0.000 description 33
- 244000061458 Solanum melongena Species 0.000 description 22
- 235000002597 Solanum melongena Nutrition 0.000 description 22
- 239000000243 solution Substances 0.000 description 11
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 239000000463 material Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 238000002156 mixing Methods 0.000 description 5
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 4
- 239000003546 flue gas Substances 0.000 description 4
- 239000003205 fragrance Substances 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 3
- 230000000638 stimulation Effects 0.000 description 3
- 235000005979 Citrus limon Nutrition 0.000 description 2
- 244000131522 Citrus pyriformis Species 0.000 description 2
- 241000222065 Lycoperdon Species 0.000 description 2
- 241000768494 Polymorphum Species 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 125000003118 aryl group Chemical group 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 235000010980 cellulose Nutrition 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 239000001814 pectin Substances 0.000 description 2
- 229920001277 pectin Polymers 0.000 description 2
- 235000010987 pectin Nutrition 0.000 description 2
- 239000002304 perfume Substances 0.000 description 2
- 230000035807 sensation Effects 0.000 description 2
- 235000019615 sensations Nutrition 0.000 description 2
- 210000000697 sensory organ Anatomy 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 208000001889 Acid-Base Imbalance Diseases 0.000 description 1
- 206010013911 Dysgeusia Diseases 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- -1 compound compound Chemical class 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000019505 tobacco product Nutrition 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Abstract
The invention discloses a method for preparing cigar tobacco leaves by means of the fermentation effect of a compounded enzyme preparation. Petioles, of tobacco leaves, which are subjected to a moisture regaining process are arranged in a compounded enzyme preparation aqueous solution for 10-30 minutes, the compounded enzyme preparation aqueous solution is sprayed on the tobacco leaves, the weight ratio of the compounded enzyme preparation aqueous solution to the tobacco leaves is 1:5-15, the tobacco leaves the water content of which is 25+/-1% are fermented for 10-60 days under the conditions that the temperature is 45-55 DEG C and the humidity is 65-75%, and the cigar tobacco leaves are obtained. According to the invention, the biological enzyme preparation and the raw materials of the cigar tobacco leaves are fermented at the same time, on the premise that a traditional flavouring manner is not used, the product quality is changed, and the tobacco leaves processed by the above processing technology are advantaged in that the bitter taste is obviously reduced, the characteristics such as flavor, smoke, taste, combustibility and grayness are all improved, and the quality of the cigar tobacco leaves is improved.
Description
Technical field
The present invention relates to cigar manufacture field, utilize compounded enzyme preparate to ferment in particular to one
The method preparing cigar tobacco.
Background technology
Cigar tobacco leaf raw material has sugared nitrogen than low feature, the meeting when burning and sucking of usual glucide
Producing acidic materials, nitrogen substance can produce alkaline matter when burning and sucking, and cigar is when burning and sucking
Owing to acid base imbalance can produce the most pungent, pained sensation, affect product sales volume.
The ultimate constituent of raw tobacco material contains cellulose, pectin, starch, protein, but
It is that the sucking quality of tobacco product is adversely affected by the most to a certain extent.Enzyme
Cellulose, pectin, starch, protein degradation can be little molecule thing by preparation under suitable conditions
Matter, such as monosaccharide, oligosaccharide, enzyme preparation, peptides.These catabolites the most inherently wind
Taste material, on the other hand can be converted into flavor components by Maillard reaction.There are some researches show,
In cigarette combustion process, unreacted saccharide completely can be transferred directly in main flume, and this
Transfer likely can bring " sweet ", " promoting the production of body fluid " and the mouthfeel of " aftertaste " for Medicated cigarette.Nicotiana tabacum L.
During modulating, be aged, process and burning and sucking, all along with non-enzymatic brown in various degree
Change reaction, produce many flavor components.
Biological enzyme technology has been widely applied to each neck such as medicine, food fermentation and feedstuff at present
Territory.But, the most do not utilize enzyme preparation to improve grinding of cigar raw material this respect
Study carefully.
Summary of the invention
The technical problem to be solved is just to provide one and utilizes compounded enzyme preparate to ferment
The method preparing cigar tobacco.The method prepares cigar tobacco and overcomes because of cigar cigarette
Leaf raw material sugar nitrogen than low, alcoholization the cycle long；Sugar nitrogen than low Nicotiana tabacum L. when burning and sucking due to soda acid not
Balance can produce pungent, pained sensation, and the shortcoming affecting product sales volume, by compound enzyme system
Agent fermentation reduces the content of unfavorable composition in Nicotiana tabacum L., is allowed to be converted into beneficially Nicotiana tabacum L. and inhales the thing of taste
Matter, Balancing degree, effectively improves the quality that burns and sucks of Nicotiana tabacum L., improves the use value of Nicotiana tabacum L..
For achieving the above object, the one that the present invention provides utilizes combined enzyme fermentation preparation snow
The method of eggplant Nicotiana tabacum L., comprises the following steps:
1) under field conditions (factors), the former Nicotiana tabacum L. of cigar is refined, and under vacuum to former Nicotiana tabacum L.
Carry out moisture regain process；
2) 15～the fiber of the α-amylase of 25%, 15～25% are weighed than meter by weight percent
Element enzyme, 15～the peroxidase of the pectase of 25%, 1～5%, remaining is flavor protease；
Mix homogeneously, obtains compound enzymic preparation, standby；
3) by step 2) compound enzymic preparation that obtains is dissolved in water or lemon that pH value is 6.0～7.0
In lemon acid-sodium citrate buffer solution, it is uniformly mixed, obtains compound enzymic preparation solution；
4) by step 1) moisture regain process Nicotiana tabacum L. petiole end be placed in compound enzymic preparation aqueous solution
Leaching handle 10～30min, then by weight 1 5～15 by compound enzymic preparation solution spray
On Nicotiana tabacum L., obtain the Nicotiana tabacum L. containing compound enzymic preparation；Standing makes water balance in Nicotiana tabacum L., then
The moisture content of regulation Nicotiana tabacum L. is to 25 ± 1%；
5) Nicotiana tabacum L. that moisture content is 25 ± 1% is placed in temperature be 45～55 DEG C, humidity be
Ferment 10～60 days under conditions of 65～75%；Obtain cigar tobacco.
Leaching handle will be immersed in the water by Nicotiana tabacum L. pommel, and Nicotiana tabacum L. is vertically placed, and water flows to cigarette by petiole
Leaf offshoot, enters inside Nicotiana tabacum L..Cigar tobacco first soaks handle in the solution, opens from petiole in Nicotiana tabacum L.
Beginning to grow and have the vein led to everywhere, solution enters inside Nicotiana tabacum L. from Nicotiana tabacum L. shank, enters it deep
Place reacts；Tobacco leaf surface spray solution later, the solution of tobacco leaf surface is from Nicotiana tabacum L. outer surface
Pore enters inside Nicotiana tabacum L., will react through Surface absorption.During two kinds of Nicotiana tabacum L. solution process:
Leaching handle contact range is little, but solution can reach Nicotiana tabacum L. vein depths；Surface sprinkling makes Nicotiana tabacum L. contact
Solution range is big, but processes only Nicotiana tabacum L. top layer and cannot arrive depths.By Nicotiana tabacum L. first carry out soak handle,
Spraying on surface, the two combines and makes solution by Nicotiana tabacum L. either range or the degree of depth again
All fully absorb.
Further, described step 2) in, compound compound enzymic preparation is by weight percent than meter
α-amylase by 20%, the cellulase of 20%, the pectase of 20%, the peroxidating of 5%
Thing enzyme, the flavor protease of 35%.
Yet further, described step 3) in, water is deionized water or pure water.
Yet further, described step 4) in, the content of each component enzymes in detection Nicotiana tabacum L., and
Make in every gram of Nicotiana tabacum L. containing compound enzymic preparation, α-amylase, cellulase, pectase, wind
The enzymatic activity of taste protease and peroxidase be respectively 3～7.5U, 15～60U, 50～100U,
100～150U and 0.4～0.6U.
Yet further, described step 4) in, compound enzymic preparation aqueous solution and the weight of Nicotiana tabacum L.
Ratio is 1 10.
Yet further, described step 4) in, every gram contains in the Nicotiana tabacum L. of compound enzymic preparation,
The enzymatic activity of α-amylase, cellulase, pectase, flavor protease and peroxidase is divided
Wei 5U, 45U, 75U, 120U and 0.5U.
Yet further, described step 4) in, the time of standing is 12～24h.
The beneficial effects of the present invention is:
The present invention uses biological enzyme formulation and cigar tobacco raw material co-fermentation, is not using tradition
Change product quality on the premise of perfuming mode, the Nicotiana tabacum L. after processing by processing technique of the present invention,
Bitterness improves substantially, obtains in odor characteristic, smoke transportation, taste characteristics, flammability and Lycoperdon polymorphum Vitt
Being improved on Fen, quality significantly improves.
Detailed description of the invention
In order to preferably explain the present invention, it is further elucidated with the present invention below in conjunction with specific embodiment
Main contents, but present disclosure is not limited solely to following example.
Embodiment 1
The method utilizing combined enzyme fermentation to prepare cigar tobacco, comprises the following steps:
1) the cigar eggplant core upper smoke of 30kg is refined 1 year, under field conditions (factors) then very
Under empty condition, upper smoke is carried out moisture regain process；
2) α-amylase of 5g, the cellulase of 5g, the pectase of 5g, 1.25g are weighed
Peroxidase and the flavor protease of 8.75g；Mix homogeneously, obtains compound enzymic preparation,
Standby；
3) by step 2) compound enzymic preparation that obtains is dissolved in deionized water, obtains mixing all
Even, obtain compound enzymic preparation aqueous solution；
4) by step 1) moisture regain process Nicotiana tabacum L. petiole end be placed in compound enzymic preparation aqueous solution
Leaching handle 30min, then by weight 1 10 by compound enzymic preparation aqueous solution spray at cigarette
Ye Shang, obtains the Nicotiana tabacum L. containing compound enzymic preparation；The content of each component enzymes in detection Nicotiana tabacum L., and
Make in every gram of Nicotiana tabacum L. containing compound enzymic preparation, α-amylase, cellulase, pectase, wind
The enzymatic activity of taste protease and peroxidase is respectively 7U, 55U, 90U, 150U and 0.6U,
Stand 24h, make water balance in Nicotiana tabacum L., then regulate the moisture content of Nicotiana tabacum L. to 25%；
5) Nicotiana tabacum L. that moisture content is 25% is placed in temperature be 52 DEG C, under the conditions of humidity is 70%
Ferment 30 days；Obtain cigar tobacco 1.
Cigar eggplant core upper smoke is usually used as cigar concentration type materials, and strength is big, heavy bitter taste.Cigarette
Leaf processes the cigar tobacco 1 obtained through combined enzyme fermentation, and the change of its flue gas style is little,
Bitterness substantially alleviates, and flue gas concentration more reduces, and its miscellaneous QI, zest, strength are the most not
Alleviate with degree.
Embodiment 2
The method utilizing combined enzyme fermentation to prepare cigar tobacco, comprises the following steps:
1) cigarette in the middle part of the cigar eggplant core of 30kg is refined 1 year, under field conditions (factors) then very
Under empty condition, upper smoke is carried out moisture regain process；
2) α-amylase of 5g, the cellulase of 5g, the pectase of 5g, 1.25g are weighed
Peroxidase and the flavor protease of 8.75g；Mix homogeneously, obtains compound enzymic preparation,
Standby；
3) by step 2) compound enzymic preparation that obtains is dissolved in deionized water, obtains mixing all
Even, obtain compound enzymic preparation aqueous solution；
4) by step 1) moisture regain process Nicotiana tabacum L. petiole end be placed in compound enzymic preparation aqueous solution
Leaching handle 25min, then by weight 1 10 by compound enzymic preparation aqueous solution spray at cigarette
Ye Shang, obtains the Nicotiana tabacum L. containing compound enzymic preparation；The content of each component enzymes in detection Nicotiana tabacum L., and
Make in every gram of Nicotiana tabacum L. containing compound enzymic preparation, α-amylase, cellulase, pectase, wind
The enzymatic activity of taste protease and peroxidase is respectively 5U, 45U, 75U, 120U and 0.5U,
Stand 24h, make water balance in Nicotiana tabacum L., then regulate the moisture content of Nicotiana tabacum L. to 24%；
5) Nicotiana tabacum L. that moisture content is 24% is placed in temperature be 50 DEG C, under the conditions of humidity is 70%
Ferment 25 days；Obtain cigar tobacco 2.
In the middle part of cigar eggplant core, cigarette is usually used as the main source of a cigar fragrance.Through compound enzyme system
The cigar tobacco 2 that agent fermentation process obtains, its aromatic style changing features is inconspicuous, cigar
Distinctive bitterness substantially alleviates, and fragrance enriches degree and is obviously improved, miscellaneous QI, zest,
Strength has alleviated, and its use value is greatly promoted.
Embodiment 3
The method utilizing combined enzyme fermentation to prepare cigar tobacco, comprises the following steps:
1) the cigar eggplant core bottom cigarette of 30kg is refined 1 year, under field conditions (factors) then very
Under empty condition, upper smoke is carried out moisture regain process；
2) α-amylase of 5g, the cellulase of 5g, the pectase of 5g, 1.25g are weighed
Peroxidase and the flavor protease of 8.75g；Mix homogeneously, obtains compound enzymic preparation,
Standby；
3) by step 2) compound enzymic preparation that obtains is dissolved in deionized water, obtains mixing all
Even, obtain compound enzymic preparation aqueous solution；
4) by step 1) moisture regain process Nicotiana tabacum L. petiole end be placed in compound enzymic preparation aqueous solution
Leaching handle 10min, then by weight 1 10 by compound enzymic preparation aqueous solution spray at cigarette
Ye Shang, obtains the Nicotiana tabacum L. containing compound enzymic preparation；The content of each component enzymes in detection Nicotiana tabacum L., and
Make in every gram of Nicotiana tabacum L. containing compound enzymic preparation, α-amylase, cellulase, pectase, wind
The enzymatic activity of taste protease and peroxidase is respectively 4U, 15U, 50U, 120U and 0.4U,
Stand 24h, make water balance in Nicotiana tabacum L., then regulate the moisture content of Nicotiana tabacum L. to 24%；
5) Nicotiana tabacum L. that moisture content is 24% is placed in temperature be 48 DEG C, under the conditions of humidity is 70%
Ferment 15 days；Obtain cigar tobacco 3.
Cigar eggplant core bottom cigarette is mainly as cigar inserts, it is desirable to flammability is good, miscellaneous QI, thorn
Swash, bitterness light, to cigar entirety sense organ without negative effect, generally its bitterness, miscellaneous QI,
Stimulate relatively eggplant core top, middle part cigarette lighter.Nicotiana tabacum L. processes the snow obtained through combined enzyme fermentation
Eggplant Nicotiana tabacum L. 3, original slightly bitter taste, miscellaneous QI, stimulation almost disappear, and other aesthetic qualities become
Change little obvious.
Embodiment 4
The method utilizing combined enzyme fermentation to prepare cigar tobacco, comprises the following steps:
1) cigar coat of 20kg is refined 1 year under field conditions (factors), then at vacuum condition
Under upper smoke is carried out moisture regain process；
2) α-amylase of 5g, the cellulase of 5g, the pectase of 5g, 1.25g are weighed
Peroxidase and the flavor protease of 8.75g；Mix homogeneously, obtains compound enzymic preparation,
Standby；
3) by step 2) compound enzymic preparation that obtains is dissolved in deionized water, obtains mixing all
Even, obtain compound enzymic preparation aqueous solution；
4) by step 1) moisture regain process Nicotiana tabacum L. petiole end be placed in compound enzymic preparation aqueous solution
Leaching handle 15min, then by weight 1 10 by compound enzymic preparation aqueous solution spray at cigarette
Ye Shang, obtains the Nicotiana tabacum L. containing compound enzymic preparation；The content of each component enzymes in detection Nicotiana tabacum L., and
Make in every gram of Nicotiana tabacum L. containing compound enzymic preparation, α-amylase, cellulase, pectase, wind
The enzymatic activity of taste protease and peroxidase is respectively 6U, 30U, 60U, 120U and 0.6U,
Stand 24h, make water balance in Nicotiana tabacum L., then regulate the moisture content of Nicotiana tabacum L. to 24%；
5) Nicotiana tabacum L. that moisture content is 24% is placed in temperature be 45 DEG C, under the conditions of humidity is 70%
Ferment 20 days；Obtain cigar tobacco 4.
Cigar coat requires flammability as the outermost packing material of cigar, usual aesthetic quality
Good, gently, appearance requirement is strict for miscellaneous QI, stimulation.Eggplant clothing is through at combined enzyme fermentation
The cigar tobacco 4 that reason obtains, the overall rufous partially of Nicotiana tabacum L., pliability strengthens, it is easy to roll,
Its aesthetic quality slightly promotes.
Embodiment 5
The method utilizing combined enzyme fermentation to prepare cigar tobacco, comprises the following steps:
1) the cigar eggplant core upper smoke of 30kg is refined 1 year, under field conditions (factors) then very
Under empty condition, upper smoke is carried out moisture regain process；
2) α-amylase of 6g, the cellulase of 6g, the pectase of 6g, 0.6g are weighed
Peroxidase and the flavor protease of 21.6g；Mix homogeneously, obtains compound enzymic preparation, standby
With；
3) by step 2) compound enzymic preparation that obtains be dissolved in pure in, be uniformly mixed,
Obtain compound enzymic preparation aqueous solution；
4) by step 1) moisture regain process Nicotiana tabacum L. petiole end be placed in compound enzymic preparation aqueous solution
Leaching handle 30min, then by weight 15 by compound enzymic preparation aqueous solution spray at cigarette
Ye Shang, obtains the Nicotiana tabacum L. containing compound enzymic preparation；The content of each component enzymes in detection Nicotiana tabacum L., and
Make in every gram of Nicotiana tabacum L. containing compound enzymic preparation, α-amylase, cellulase, pectase, wind
The enzymatic activity of taste protease and peroxidase be respectively 7.5U, 60U, 100U, 150U and
0.6U, stands 12h, makes water balance in Nicotiana tabacum L., then regulate the moisture content of Nicotiana tabacum L. to 26%；
5) Nicotiana tabacum L. that moisture content is 25% is placed in temperature be 55 DEG C, under the conditions of humidity is 65%
Ferment 60 days；Obtain cigar tobacco 5.
Cigar eggplant core upper smoke is usually used as cigar concentration type materials, and strength is big, heavy bitter taste.Cigarette
Leaf processes the cigar tobacco 5 obtained through combined enzyme fermentation, and the change of its flue gas style is little,
Bitterness substantially alleviates, and flue gas concentration more reduces, and its miscellaneous QI, zest, strength are the most not
Alleviate with degree.
Embodiment 6
The method utilizing combined enzyme fermentation to prepare cigar tobacco, comprises the following steps:
1) cigarette in the middle part of the cigar eggplant core of 30kg is refined 1 year, under field conditions (factors) then very
Under empty condition, upper smoke is carried out moisture regain process；
2) α-amylase of 6g, the cellulase of 6g, the pectase of 6g, 1.2g are weighed
Peroxidase and the flavor protease of 5.76g；Mix homogeneously, obtains compound enzymic preparation, standby
With；
3) by step 2) compound enzymic preparation that obtains be dissolved in pH value be 7 buffer solution in,
It is uniformly mixed, obtains compound enzymic preparation solution；
4) by step 1) moisture regain process Nicotiana tabacum L. petiole end be placed in compound enzymic preparation aqueous solution
Leaching handle 25min, then by weight 1 15 by compound enzymic preparation aqueous solution spray at cigarette
Ye Shang, obtains the Nicotiana tabacum L. containing compound enzymic preparation；The content of each component enzymes in detection Nicotiana tabacum L., and
Make in every gram of Nicotiana tabacum L. containing compound enzymic preparation, α-amylase, cellulase, pectase, wind
The enzymatic activity of taste protease and peroxidase is respectively 3U, 15U, 50U, 150U and 0.6U,
Stand 24h, make water balance in Nicotiana tabacum L., then regulate the moisture content of Nicotiana tabacum L. to 25%；
5) Nicotiana tabacum L. that moisture content is 24% is placed in temperature be 50 DEG C, under the conditions of humidity is 70%
Ferment 25 days；Obtain cigar tobacco 6.
In the middle part of cigar eggplant core, cigarette is usually used as the main source of a cigar fragrance.Through compound enzyme system
The cigar tobacco 6 that agent fermentation process obtains, its aromatic style changing features is inconspicuous, cigar
Distinctive bitterness substantially alleviates, and fragrance enriches degree and is obviously improved, miscellaneous QI, zest,
Strength has alleviated, and its use value is greatly promoted.
Embodiment 7
The method utilizing combined enzyme fermentation to prepare cigar tobacco, comprises the following steps:
1) the cigar eggplant core bottom cigarette of 30kg is refined 1 year, under field conditions (factors) then very
Under empty condition, upper smoke is carried out moisture regain process；
2) α-amylase of 5g, the cellulase of 6.5g, the pectase of 6g, 1.15g are weighed
Peroxidase and the flavor protease of 7.45g；Mix homogeneously, obtains compound enzymic preparation,
Standby；
3) by step 2) compound enzymic preparation that obtains is dissolved in deionized water, obtains mixing all
Even, obtain compound enzymic preparation aqueous solution；
4) by step 1) moisture regain process Nicotiana tabacum L. petiole end be placed in compound enzymic preparation aqueous solution
Leaching handle 30min, then by weight 1 15 by compound enzymic preparation aqueous solution spray at cigarette
Ye Shang, obtains the Nicotiana tabacum L. containing compound enzymic preparation；The content of each component enzymes in detection Nicotiana tabacum L., and
Make in every gram of Nicotiana tabacum L. containing compound enzymic preparation, α-amylase, cellulase, pectase, wind
The enzymatic activity of taste protease and peroxidase is respectively 6U, 35U, 80U, 130U and 0.5U,
Stand 24h, make water balance in Nicotiana tabacum L., then regulate the moisture content of Nicotiana tabacum L. to 25%；
5) Nicotiana tabacum L. that moisture content is 25% is placed in temperature be 48 DEG C, under the conditions of humidity is 70%
Ferment 15 days；Obtain cigar tobacco 7.
Cigar eggplant core bottom cigarette is mainly as cigar inserts, it is desirable to flammability is good, miscellaneous QI, thorn
Swash, bitterness light, to cigar entirety sense organ without negative effect, generally its bitterness, miscellaneous QI,
Stimulate relatively eggplant core top, middle part cigarette lighter.Nicotiana tabacum L. processes the snow obtained through combined enzyme fermentation
Eggplant Nicotiana tabacum L. 7, original slightly bitter taste, miscellaneous QI, stimulation almost disappear, and other aesthetic qualities become
Change little obvious.
Raw material in above-described embodiment is all purchased from market, wherein, α-amylase (enzymatic activity
40000U/g), cellulase (enzymatic activity 30000U/g), pectase (enzymatic activity 30000U/g),
Flavor protease (enzymatic activity 50000U/g), peroxidase (enzymatic activity 150000U/g)
Cigar eggplant core (upper smoke, middle part cigarette, bottom cigarette) and cigar coat are all imported articles.
Smoke panel test test
By do not entered combined enzyme fermentation process cigar eggplant core (upper smoke, middle part cigarette, under
Portion's cigarette), the cigar tobacco 1～7 for preparing of cigar coat and embodiment 1～7 prepare snow respectively
Eggplant cigarette, carry out smokeing panel test under the same conditions effect monitoring by these 11 kinds of cigars；Monitoring result
As shown in table 1 below:
1 11 kinds of cigars of table carry out effect table 1 of smokeing panel test under the same conditions
2 11 kinds of cigars of table carry out effect table 2 of smokeing panel test under the same conditions
| Cigar | Aroma quality | Perfume quantity | Miscellaneous QI | Zest |
| Untreated upper smoke | 4 | 4 | 4 | 4 |
| Embodiment 1 | 4 | 3.5 | 4 | 4.5 |
| Embodiment 5 | 4 | 3.5 | 4 | 4 |
| Untreated middle part cigarette | 4 | 3 | 4 | 4 |
| Embodiment 2 | 3.5 | 2.5 | 4 | 5 |
| Embodiment 6 | 3.5 | 2.5 | 4 | 5 |
| Untreated bottom cigarette | 3 | 3 | 4 | 3 |
| Embodiment 3 | 3 | 3 | 4 | 3 |
| Embodiment 7 | 3 | 3 | 4 | 3 |
| Untreated cigar coat | 3 | 3 | 4 | 4 |
| Embodiment 4 | 3 | 2.5 | 4 | 4.5 |
Note
Table 3 is smoked panel test standard
As shown in Tables 1 and 2: by do not entered combined enzyme fermentation process cigar eggplant core (on
Portion's cigarette, middle part cigarette, bottom cigarette), cigar coat is compared with obtaining cigar by fermentation；Real
The cigar tobacco executing example 1～7 prepares the aroma quality of cigar, perfume quantity, pleasant impression, sugariness
Significantly improving, miscellaneous QI and zest substantially reduce, and combustibility increases, cigar total
Body quality entirety improves.Processing through combined enzyme fermentation and obtain cigar tobacco, its bitterness changes
Kind obvious, in odor characteristic, smoke transportation, taste characteristics, flammability and Lycoperdon polymorphum Vitt score all
Being improved, quality significantly improves.
Other unspecified part is prior art.Although above-described embodiment is to the present invention
It is made that detailed description, but its a part of embodiment that is only the present invention rather than all real
Executing example, people can also obtain other according to the present embodiment under without creative premise and implement
Example, these embodiments broadly fall into scope.
Claims (7)
1. one kind utilizes the method that combined enzyme fermentation prepares cigar tobacco, it is characterised in that:
Comprise the following steps:
1) under field conditions (factors), the former Nicotiana tabacum L. of cigar is refined, and under vacuum to former Nicotiana tabacum L.
Carry out moisture regain process；
2) 15～the fiber of the α-amylase of 25%, 15～25% are weighed than meter by weight percent
Element enzyme, 15～the peroxidase of the pectase of 25%, 1～5%, remaining is flavor protease；
Mix homogeneously, obtains compound enzymic preparation, standby；
3) by step 2) compound enzymic preparation that obtains is soluble in water, mix homogeneously, answered
Synthase formulation soln；
4) by step 1) moisture regain process Nicotiana tabacum L. petiole end be placed in compound enzymic preparation solution leaching
Handle 10～30min, then exists compound enzymic preparation solution spray by weight 1 5～15
On Nicotiana tabacum L., obtain the Nicotiana tabacum L. containing compound enzymic preparation；Standing makes water balance in Nicotiana tabacum L., then adjusts
The moisture content of joint Nicotiana tabacum L. is to 25 ± 1%；
5) Nicotiana tabacum L. that moisture content is 25 ± 1% is placed in temperature be 45～55 DEG C, humidity be
Ferment 10～60 days under conditions of 65～75%；Obtain cigar tobacco.
Combined enzyme fermentation is utilized to prepare the side of cigar tobacco the most according to claim 1
Method, it is characterised in that: described step 2) in, compound enzymic preparation by weight percent than meter by
The α-amylase of 20%, the cellulase of 20%, the pectase of 20%, the peroxidase of 5%
With 35% flavor protease form.
The most according to claim 1 or claim 2 combined enzyme fermentation is utilized to prepare cigar tobacco
Method, it is characterised in that: described step 3) in, water is deionized water or pure water.
The most according to claim 1 or claim 2 combined enzyme fermentation is utilized to prepare cigar tobacco
Method, it is characterised in that: described step 4) in, the content of each component enzymes in detection Nicotiana tabacum L.,
And make in every gram of Nicotiana tabacum L. containing compound enzymic preparation, α-amylase, cellulase, pectase,
The enzymatic activity of flavor protease and peroxidase be respectively 3～7.5U, 15～60U,
50～100U, 100～150U and 0.4～0.6U.
Combined enzyme fermentation is utilized to prepare the side of cigar tobacco the most according to claim 4
Method, it is characterised in that: described step 4) in, compound enzymic preparation solution and the weight ratio of Nicotiana tabacum L.
It is 1 10.
Combined enzyme fermentation is utilized to prepare the side of cigar tobacco the most according to claim 5
Method, it is characterised in that: described step 4) in, every gram contains in the Nicotiana tabacum L. of compound enzymic preparation,
The enzymatic activity of α-amylase, cellulase, pectase, flavor protease and peroxidase is divided
Wei 5U, 45U, 75U, 120U and 0.5U.
7. utilize combined enzyme fermentation to prepare cigar tobacco according to claim 5 or 6
Method, it is characterised in that: it is characterized in that: described step 4) in, the time of standing is
12～24h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510359765.9A CN105054286B (en) | 2015-06-25 | 2015-06-25 | Method for preparing cigar tobacco leaves by means of fermentation effect of compounded enzyme preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510359765.9A CN105054286B (en) | 2015-06-25 | 2015-06-25 | Method for preparing cigar tobacco leaves by means of fermentation effect of compounded enzyme preparation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105054286A CN105054286A (en) | 2015-11-18 |
| CN105054286B true CN105054286B (en) | 2017-01-11 |
Family
ID=54484001
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510359765.9A Active CN105054286B (en) | 2015-06-25 | 2015-06-25 | Method for preparing cigar tobacco leaves by means of fermentation effect of compounded enzyme preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105054286B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106136304B (en) * | 2016-07-21 | 2017-11-28 | 云南中烟工业有限责任公司 | A kind of cigarette flavors additive and preparation method and application |
| CN108354217A (en) * | 2018-03-14 | 2018-08-03 | 红云红河烟草(集团)有限责任公司 | A kind of saccharification processing method of offal |
| CN110742303A (en) * | 2019-10-22 | 2020-02-04 | 红塔辽宁烟草有限责任公司 | Complex enzyme preparation for improving smoking quality of tobacco flakes and application thereof |
| CN110833206A (en) * | 2019-11-18 | 2020-02-25 | 四川中烟工业有限责任公司 | Tobacco leaf processing method for cigar |
| KR102267982B1 (en) * | 2020-07-09 | 2021-06-21 | 주식회사 케이티앤지 | Vanilla smoking material wrapper and smoking article containing same |
| CN113679091B (en) * | 2021-09-08 | 2022-09-30 | 湖北中烟工业有限责任公司 | Preparation method of leaf bundle type cigar core |
| CN113679089A (en) * | 2021-09-08 | 2021-11-23 | 湖北中烟工业有限责任公司 | Processing technology of tobacco leaves for eggplant coat and eggplant sleeve |
| CN114403496B (en) * | 2022-01-25 | 2023-03-28 | 湖北省烟草科学研究院 | Synergist for improving combustibility and ash gray level of fermented cigar tobacco leaves and application method thereof |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4307733A (en) * | 1978-09-20 | 1981-12-29 | Philip Morris, Inc. | Process for the treatment of tobacco materials |
| CN100556323C (en) * | 2007-09-30 | 2009-11-04 | 川渝中烟工业公司 | The method for alcoholizing of cigar tobacco |
| CN101731746B (en) * | 2008-11-11 | 2012-04-25 | 安徽中烟工业公司 | Method for fermenting Baimao tobacco |
| CN102178344B (en) * | 2011-05-13 | 2012-10-03 | 川渝中烟工业有限责任公司 | Method for reducing protein of cigar core leaves |
| CN103070475B (en) * | 2013-01-10 | 2015-05-20 | 川渝中烟工业有限责任公司 | Cigar tobacco leaf additive and production method thereof |
| CN103120362B (en) * | 2013-02-28 | 2015-02-04 | 湖北中烟工业有限责任公司 | Method for improving combustibility of cigar tobacco leaves |
-
2015
- 2015-06-25 CN CN201510359765.9A patent/CN105054286B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN105054286A (en) | 2015-11-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105054286B (en) | Method for preparing cigar tobacco leaves by means of fermentation effect of compounded enzyme preparation | |
| CN106833898B (en) | Preparation method of rice-flavor tobacco flavor | |
| CN106235375A (en) | A kind of method of the suncured tabacco reconstituted tobacco manufacturing applicable flue-cured tobacco local flavor | |
| CN104705779B (en) | A kind of processing method improving cut stem quality | |
| CN101564201B (en) | Method for improving quality of tobacco leaves by enzyme treatment in tobacco shred making processing technology | |
| CN105029685B (en) | Chinese herbal medicine extract and enzyme preparation application in cigar fermentation upgrading | |
| CN102178344B (en) | Method for reducing protein of cigar core leaves | |
| CN106047481A (en) | Preparation method and application of discarded tobacco leaf fermentation extract | |
| CN103462212B (en) | Method for improving quality of tobacco leaves by adding enzyme preparation, glucose and citric acid | |
| CN108968132B (en) | Preparation method of reconstituted tobacco coating liquid | |
| CN105029686B (en) | Method for preparing cigar tobacco leaves by virtue of oxidase fermentation | |
| CN104997157A (en) | Application of flavourzyme for fermenting and upgrading a cigar | |
| CN104872813B (en) | Utilize the method that cellulase fermentations prepares cigar tobacco | |
| CN113892666B (en) | Enzymolysis quality-improving treatment method for cigar core tobacco leaves | |
| CN109349678B (en) | Preparation method and application of composite tobacco leaf extract | |
| CN105029683B (en) | The method that cigar tobacco is prepared using composite ferment fermentation | |
| CN104886763A (en) | Application of amylase in cigar fermentation and quality improvement | |
| CN105029684B (en) | Utilize the method that compounded amino acid fermentation prepares cigar tobacco | |
| CN104188090A (en) | Method for reducing carbonic oxide emission of paper-making reconstituted tobacco | |
| CN111588074A (en) | Tobacco leaf aroma-enhancing and thorn-reducing treatment method | |
| CN105831800A (en) | Compound enzyme preparation for improving tobacco quality and application thereof | |
| CN101683181B (en) | Method for preparing cut stems used for cigarettes | |
| CN105713924A (en) | Method for preparing fermented type tobacco spice from rosa sterilis | |
| CN105831799A (en) | Method for preparing cigarette tobacco suitable for snus and application of cigarette tobacco | |
| CN110169587A (en) | A kind of enzyme preparation application method for making to generate cinnabar shape maturation spot after tobacco leaf is roasting |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |