CN115053851B - Device for delaying metamorphosis of bivalve larvae and application method thereof - Google Patents

Device for delaying metamorphosis of bivalve larvae and application method thereof Download PDF

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CN115053851B
CN115053851B CN202210575558.7A CN202210575558A CN115053851B CN 115053851 B CN115053851 B CN 115053851B CN 202210575558 A CN202210575558 A CN 202210575558A CN 115053851 B CN115053851 B CN 115053851B
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water
cylindrical barrel
larvae
pipe
module
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CN115053851A (en
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杜美荣
蒋增杰
吴彪
姜娓娓
房景辉
高亚平
蔺凡
李瑞环
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Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
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Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
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Priority to JP2023083540A priority patent/JP7421851B2/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/003Aquaria; Terraria
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/50Culture of aquatic animals of shellfish
    • A01K61/54Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/80Feeding devices
    • A01K61/85Feeding devices for use with aquaria
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/04Arrangements for treating water specially adapted to receptacles for live fish
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/06Arrangements for heating or lighting in, or attached to, receptacles for live fish
    • A01K63/065Heating or cooling devices
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention discloses a device for delaying metamorphosis of bivalve larvae and a use method thereof, belonging to the field of biological experimental devices, wherein the device comprises a larva cultivation pool, a temperature control system, a timing feeding module, a timing water changing module, a vortex delaying metamorphosis module and a sewage disposal module, wherein the larva cultivation pool is a cylindrical barrel body, and the vortex delaying metamorphosis module comprises an inflator pump, an inflation tube and an adjusting valve; the vortex delay metamorphosis module forms vortex which rotates along the circumferential direction of the cylindrical barrel in the cylindrical barrel through uninterrupted inflation; the device can prolong the creeping period of the larvae, delay metamorphosis and prolong the time for acquiring the experimental materials of the larvae. The temperature control is accurate, the use is convenient, the survival rate of the larvae is improved, the activity of the cultivated larvae is high, the ingestion state is good, and the development of the next experiment is facilitated; the device has high automation degree, reduces the participation degree of people, reduces the workload, saves the manpower, has high integration degree, and is convenient to carry and transport.

Description

Device for delaying metamorphosis of bivalve larvae and application method thereof
Technical Field
The invention belongs to the field of biological experimental devices, and particularly relates to a device for delaying metamorphosis of bivalve larvae and a use method thereof
Background
Bivalve shellfish is an important offshore culture variety in China, the culture yield is 63.38% of the mariculture yield in China, 91.44% of the mariculture yield in China (fishery statistics annual survey, 2021), and the obtaining of a large number of seedlings is inexhaustible for large-scale culture. Larval development of bivalve shellfish undergoes planktonic and benthic life. Bivalve shellfish is oviparous, and from egg development to juvenile mollusc undergoes embryo stage, planktonic larva stage and settlement stage respectively. The embryo stage and planktonic larvae are suspended in seawater for planktonic life, and the planktonic time varies from a few days to twenty-several days according to different species. The bivalve shellfish larva is sensitive to environmental changes in the cultivation process, and a large number of larvae die due to the change of certain physicochemical parameters, so that the researches on proper environmental parameters of embryos and larvae and basic biology of larvae are important to aquaculture production and scientific research. For marine organism scientific research students, enough larvae with good states can be obtained as the first step of carrying out scientific research experiments, the time for obtaining floating larvae is limited to about a short few days to ten days due to limitation of biological learning of the larvae, if experiments are not smooth in the current year, the experiment cannot be basically repeated, experimental batches are increased or the experimental direction is adjusted, enough experimental larvae materials can be obtained only in the propagation season of the next year, the trouble of obtaining the experimental materials is extremely unfavorable for the scientific research work of bivalve shellfish larvae, so that many scientific research works cannot be carried out smoothly, and the progress of the scientific research is seriously retarded. Therefore, a culture device which is automatic, temperature-controlled and capable of prolonging the culture time of bivalve larvae and delaying metamorphosis has to be developed.
Disclosure of Invention
The invention aims to solve the technical problem of providing a device for delaying metamorphosis of bivalve larvae and a use method thereof, wherein the device can automatically control temperature, automatically feed water at fixed time, form vortex flow along the barrel wall and the like, realize automatic control during larva cultivation, lighten workload, prolong larva cultivation time, and achieve the purposes of delaying larva metamorphosis and prolonging acquisition of larva experimental materials.
The invention is carried out by the following technical scheme:
the device for delaying the metamorphosis of the bivalve larvae comprises a larva cultivating pool, a temperature control system, a timing feeding module, a timing water changing module, a vortex delayed metamorphosis module and a sewage discharging module,
the larva cultivating pond is a cylindrical barrel body and is provided with a grabbing handle for moving the cylindrical barrel and standing feet placed on the ground;
the vortex delay metamorphosis module comprises an inflator pump, an inflation tube and an adjusting valve; the cylindrical barrel is horizontally placed, the inflator pump is connected to gas tube one end, the other end stretches into along the cylindrical barrel inside, extend to the other end from one end along cylindrical barrel lateral wall, the gas tube is equipped with along gas tube axial arrangement's venthole apart from cylindrical barrel inner wall side, the bubble that comes out from the venthole rises to the top along cylindrical barrel inside, uninterruptedly aerifys, then form along cylindrical barrel circumference rotatory vortex in cylindrical barrel inside, the vortex reduces the creeping opportunity of larva at barrel wall's creeping, the extension larva creeping period, delay larva is metamorphosis.
Further, the device also comprises a temperature control system, wherein the temperature control system comprises a cylindrical sleeve, a water inlet pipe, a water outlet pipe and a circulating water temperature controller; the cylindrical sleeve is sleeved on the periphery of the cylindrical barrel, the water outlet pipe is connected to the water inlet of the circulating water temperature controller, the water inlet pipe is connected to the water outlet of the circulating water temperature controller, water flows out from the bottom of the cylindrical barrel through the water outlet pipe, enters the water inlet pipe after being heated by the circulating water temperature controller, and finally flows into the cylindrical barrel;
further, the device also comprises a timing feeding module, wherein the timing feeding module comprises a microalgae culture bottle, a timing switch and a first peristaltic pump; the microalgae culture bottle is sequentially connected with a timing switch and a first peristaltic pump through a connecting pipe, and finally enters the cylindrical barrel; the first peristaltic pump pipe which is disinfected and sterilized is connected with the microalgae culture bottle, the switching time of the first peristaltic pump is set by a first timing switch, the switching time is controlled and set according to the feeding amount of shellfish of different breeding varieties and different breeding stages, and baits are pumped into the larva breeding pond at regular time.
Further, the device comprises a timing water changing module, wherein the timing water changing module comprises a filtering sea water pool, a second peristaltic pump, a second timing switch, a water changing water inlet pipe, a first water outlet, a water drain pipe and a detachable water outlet sieve plate; the filtering sea water pool, the second peristaltic pump and the second timing switch are sequentially communicated into the cylindrical barrel through a water changing inlet pipe, a row of first water outlets are formed in the side wall of the cylindrical barrel, a detachable water outlet sieve plate is arranged on the first water outlets, a water discharging pipe is connected to the first water outlets, and a valve is arranged on the water discharging pipe. When changing water, the valve is opened, water flows out from the cylindrical barrel, and the water outlet sieve plate can be detached to prevent shellfish larvae from flowing out along with the water.
Further, the blowdown module comprises a blowdown hopper outlet, a blowdown pipe and a blowdown valve; the drain hopper opening is arranged on the side wall of the cylindrical barrel, and is connected with a drain pipe which is provided with a drain valve at the bottom of the cylindrical barrel when in use. The device is used for reducing the increase of substances such as internal feces, excreted ammonia nitrogen and the like after the larva is cultured for a long time, and when in use, the lower funnel-shaped sewage discharging valve is opened for sewage discharging treatment.
Further, the cylindrical barrel top is provided with an observation port which can be opened and closed for observing larvae in the barrel, picking and placing the larvae, or cleaning the inside of the cylindrical barrel.
The invention also provides a using method of the device, the device is connected together, the cylindrical barrel is filled with water, the vortex delay metamorphosis module is opened, bubbles come out from the air outlet and rise to the top along the interior of the cylindrical barrel, the bubbles are continuously inflated, vortex rotating along the circumferential direction of the cylindrical barrel is formed in the interior of the cylindrical barrel, the cultivation water temperature is adjusted, the temperature is 5-8 ℃ lower than the conventional cultivation temperature, shellfish larvae are placed into the cylindrical barrel through the observation port, the timing feeding module is set, the larvae are fed at fixed time, the timing water changing module is set, the rotating speed of the second peristaltic pump is set to be lower than 50 when the water is changed, the flow rate of the water inlet and the first water outlet is reduced, the vortex flow rate in the cultivation pool is increased in a matched mode to achieve the aim of less accumulation of the larvae at the screen port, after the cultivation time is long, the internal excrement and the substances such as excretion are increased, the ammonia nitrogen discharging valve at the lower part is opened, and the sewage discharging treatment is carried out.
Compared with the prior art, the invention has the beneficial effects that:
the device can prolong the creeping period of the larvae, delay metamorphosis and prolong the time for acquiring the experimental materials of the larvae. The temperature control is accurate, the use is convenient, the survival rate of the larvae is improved, the activity of the cultivated larvae is high, the ingestion state is good, and the development of the next experiment is facilitated; the device has high automation degree, reduces the participation degree of people, reduces the workload, saves the manpower, has high integration degree, and is convenient to carry and transport.
Drawings
FIG. 1 is a front view of the device of the present invention;
fig. 2 is a rear view of the device of the present invention.
1. The device comprises a cylindrical barrel, 2, a grip handle, 3, a stand, 4, an inflator pump, 5, an inflation tube, 6, a regulating valve, 7, an air outlet, 8, a water outlet tube, 9, a circulating water temperature controller, 10, a microalgae culture bottle, 11, a first timing switch, 12, a first peristaltic pump, 13, a connecting tube, 14, a filtering seawater pond, 15, a second peristaltic pump, 16, a second timing switch, 17, a water changing inlet tube, 18, a first water outlet, 19, a sieve plate, 20, a sewage drain funnel, 21, a sewage drain, 22, a sewage drain valve, 23, a water inlet tube, 24, a drain tube, 25 and an observation port.
Detailed Description
The technical scheme of the present invention is further explained by examples below, but the scope of the present invention is not limited in any way by the examples.
Example 1
A device for delaying metamorphosis of bivalve larvae is shown in figures 1 and 2, which comprises a larva cultivation pool, a temperature control pool and a temperature control system, a timing feeding module, a timing water changing module, a vortex delayed metamorphosis module and a pollution discharge module,
the larva cultivating pond is a cylindrical barrel 1, the diameter of the larva cultivating pond is 20-40 cm, and the length of the larva cultivating pond is 30-60 cm, and the larva cultivating pond is provided with a grabbing handle 2 for moving the body of the cylindrical barrel 1 and a stand 3 placed on the ground;
the vortex delay metamorphosis module comprises an inflator pump 4, an inflation tube 5 and an adjusting valve 6; the cylindrical barrel is horizontally arranged, one end of the air charging pipe 5 is connected with the air charging pump 4, the other end of the air charging pipe extends into the cylindrical barrel 1, the side wall of the cylindrical barrel 1 extends from one end to the other end, the side, which is away from the inner wall of the cylindrical barrel 1, of the air charging pipe 5 is provided with air outlet holes 7 which are axially arranged along the air charging pipe 5, air bubbles coming out of the air outlet holes 7 rise to the top along the inner part of the cylindrical barrel 1, and uninterruptedly charges, so that vortex which rotates along the circumferential direction of the cylindrical barrel 1 is formed in the cylindrical barrel 1, the creeping opportunity of larvae on the barrel wall is reduced by the vortex, the creeping period of the larvae is prolonged, and the metamorphosis of the larvae is delayed;
the device also comprises a temperature control system, wherein the temperature control system comprises a cylindrical sleeve, a water inlet pipe 23, a water outlet pipe 8 and a circulating water temperature controller 9; the cylindrical sleeve barrel is sleeved on the periphery of the cylindrical barrel 1, the water outlet pipe 8 is connected to the water inlet of the circulating water temperature controller 9, the water inlet pipe is connected to the water outlet of the circulating water temperature controller 9, water flows out from the bottom of the cylindrical barrel 1 through the water outlet pipe 8, enters the water inlet pipe after being heated by the circulating water temperature controller 9, and finally flows into the cylindrical barrel 1;
the device also comprises a timing feeding module, wherein the timing feeding module comprises a microalgae culture bottle 10, a first timing switch 11 and a first peristaltic pump 12; the microalgae culture bottle 10 is sequentially connected with a first timing switch 11 and a first peristaltic pump 12 through a connecting pipe 13, and finally enters the cylindrical barrel 1; the first peristaltic pump 12 after disinfection and sterilization is connected with the microalgae culture bottle 10 in a pipe way, the switching time of the first peristaltic pump 12 is set by the first timing switch 11, the switching time is controlled and set according to the feeding amount of shellfish in different culture varieties and different culture stages, and baits are pumped into the larva culture pond at regular time.
The device comprises a timing water changing module, wherein the timing water changing module comprises a filtering sea water pool 14, a second peristaltic pump 15, a second timing switch 16, a water changing water inlet pipe 17, a first water outlet 18 and a detachable water outlet screen plate 19; the filtering sea water pool 14, the second peristaltic pump 15 and the second timing switch 16 are sequentially communicated into the cylindrical barrel 1 through a water changing inlet pipe 17, a row of first water outlets 18 are arranged on the side wall of the cylindrical barrel 1, a detachable water outlet sieve plate 19 is arranged on the first water outlets 18, a drain pipe 24 is connected to the first water outlets 18, and a valve is arranged on the drain pipe. When changing water, the valve is opened, water flows out from the cylindrical barrel 1, and the detachable water outlet sieve plate 19 prevents shellfish larvae from flowing out along with the water.
The timing feeding and water changing system is controlled by a timer, and timing feeding of microalgae feed is realized by timing opening and closing of the timer, so that water is changed at timing.
The sewage draining module comprises a sewage draining hopper opening 20, a sewage draining pipe 21 and a sewage draining valve 22; the drain hopper opening 20 is arranged on the side wall of the cylindrical barrel 1, and in the use state, the drain hopper opening 20 is connected with a drain pipe 21 at the bottom of the cylindrical barrel 1, and a drain valve 22 is arranged on the drain pipe 21. For reducing the increase of substances such as internal feces, excreted ammonia nitrogen and the like after the long larva cultivation time, and opening the lower funnel-shaped sewage discharging valve 22 for sewage discharging treatment during use.
The top of the cylindrical barrel 1 is provided with an observation opening 25, and the observation opening 25 can be opened and closed for observing larvae in the barrel, picking and placing the larvae, or cleaning the inside of the cylindrical barrel 1.
The method for cultivating oyster by using the device comprises the steps of connecting the device, filling water into a cylindrical barrel 1, opening a vortex delay metamorphosis module, enabling bubbles to rise to the top from the inside of the cylindrical barrel 1 along an air outlet 7, forming vortex rotating along the circumferential direction of the inner wall of the cylindrical barrel 1, adjusting the cultivation water temperature to be lower than the conventional cultivation temperature by 5-8 ℃ and 17 ℃, placing shellfish larvae into the cylindrical barrel 1 through an observation port, setting a timing feeding module, timing feeding the larvae, setting a timing water changing module, setting the rotating speed of a second peristaltic pump 15 to be lower than 50 when changing water, reducing the flow speed of a water changing water inlet pipe 17 and a first water outlet 18, matching with the vortex flow speed in a cultivation pond to achieve the aim of accumulating fewer larvae at a screen port, after the cultivation time is long, causing the increase of substances such as internal excrement, excreted ammonia nitrogen and the like, and opening a drain valve 22 of a funnel at the lower part for sewage disposal treatment.
When the device is used for culturing scallop and oyster larvae, the larvae float in vortex formed in the barrel wall, the larvae can finish metamorphosis within 2 days, the settlement metamorphosis can be finished within 15 days, the survival rate reaches 85%, the time for obtaining the larvae is greatly prolonged, the experiment thinking is adjusted for scientific research workers, the experiment batch is increased, and repeated experiments are very beneficial.

Claims (3)

1. A using method of a bivalve larva delayed metamorphosis device is characterized in that the device comprises a larva cultivating pool, a temperature control system, a timing feeding module, a timing water changing module, a vortex delayed metamorphosis module and a pollution discharge module,
the larva cultivating pond is a cylindrical barrel (1) and is provided with a gripping handle (2) for moving the cylindrical barrel (1) and a stand (3) placed on the ground;
the vortex delay metamorphosis module comprises an inflator pump (4), an inflation tube (5) and an adjusting valve (6); the cylindrical barrel (1) is horizontally arranged, one end of the air charging pipe (5) is connected with the air charging pump (4), the other end of the air charging pipe extends into the cylindrical barrel (1) along the side wall of the cylindrical barrel (1) from one end to the other end, the air outlet holes (7) axially arranged along the air charging pipe (5) are formed on the inner wall side of the air charging pipe (5) away from the cylindrical barrel (1), air bubbles coming out of the air outlet holes (7) ascend to the top along the inner part of the cylindrical barrel (1) and continuously charge, so that vortex flow rotating along the circumferential direction of the cylindrical barrel (1) is formed in the cylindrical barrel (1), creeping opportunities of larvae on the barrel wall are reduced by the vortex flow, the creeping period of the larvae is prolonged, and the metamorphosis of the larvae is delayed;
the top of the cylindrical barrel (1) is provided with an observation port (25), and the observation port (25) can be opened and closed and is used for observing larvae in the barrel, taking and placing the larvae or cleaning the inside of the cylindrical barrel (1);
the timing water changing module comprises a filtering sea water pool (14), a second peristaltic pump (15), a second timing switch (16), a water changing water inlet pipe (17), a first water outlet (18), a drain pipe (24) and a detachable water outlet sieve plate (19); the seawater filtering pool (14), the second peristaltic pump (15) and the second timing switch (16) are sequentially communicated into the cylindrical barrel (1) through a water changing water inlet pipe (17), a row of first water outlets (18) are formed in the side wall of the cylindrical barrel (1), a detachable water outlet screen plate (19) is arranged on the first water outlets (18), a water discharging pipe (24) is connected to the first water outlets (18), and a valve is arranged on the water discharging pipe (24); when water is changed, a valve is opened, water flows out from the cylindrical barrel (1), and the detachable water outlet sieve plate (19) prevents shellfish larvae from flowing out along with the water;
the sewage draining module comprises a sewage draining hopper opening (20), a sewage draining pipe (21) and a sewage draining valve (22); the drain hopper opening (20) is arranged on the side wall of the cylindrical barrel (1), and in the use state, the drain hopper opening (20) is connected with a drain pipe (21), and a drain valve (22) is arranged on the drain pipe (21);
the method comprises the steps of connecting the devices together, filling the cylindrical barrel (1) with water, opening a vortex delay metamorphosis module, enabling bubbles to rise to the top along the inside of the cylindrical barrel (1) from the air outlet hole (7), continuously inflating, forming vortex which rotates along the circumferential direction of the cylindrical barrel (1) inside the cylindrical barrel (1), adjusting the temperature of cultivation water to be lower than the conventional cultivation temperature by 5-8 ℃, putting shellfish larvae into the cylindrical barrel (1) through the observation hole (25), setting a timing feeding module, timing feeding the larvae, setting a timing water changing module, setting a second peristaltic pump (15) with the rotating speed lower than 50 during water changing, reducing the flow rate of a water changing water inlet pipe (17) and a first water outlet (18), matching with increasing the vortex flow rate in the cultivation pool to achieve the aim of reducing accumulation of the larvae at the screen opening, after the cultivation time is long, causing the increase of substances including internal feces and ammonia nitrogen, and opening a lower funnel-shaped sewage discharging valve (22), and carrying out sewage discharging treatment.
2. The method according to claim 1, characterized in that the device further comprises a temperature control system comprising a cylindrical sleeve, a water inlet pipe (23), a water outlet pipe (8), a circulating water temperature controller (9); the cylindrical sleeve barrel is sleeved on the periphery of the cylindrical barrel (1), the water outlet pipe (8) is connected to the water inlet of the circulating water temperature controller (9), the water inlet pipe (23) is connected to the water outlet of the circulating water temperature controller (9), water flows out from the bottom of the cylindrical barrel (1) through the water outlet pipe (8), enters the water inlet pipe (23) after being heated by the circulating water temperature controller (9), and finally flows into the cylindrical barrel (1).
3. The method of claim 1, wherein the device further comprises a timing feeding module comprising a microalgae culture bottle (10), a timing switch, a first peristaltic pump (12); the microalgae culture bottle (10) is sequentially connected with a timing switch and a first peristaltic pump (12) through a connecting pipe (13), and finally enters the cylindrical barrel (1); the first peristaltic pump (12) after disinfection and sterilization is connected with the microalgae culture bottle (10) through a pipe, the switching time of the first peristaltic pump (12) is set by a first timing switch (11), the switching time is controlled and set according to the feeding amount of shellfish in different culture varieties and different culture stages, and baits are pumped into the larva culture pond at regular time.
CN202210575558.7A 2022-05-25 2022-05-25 Device for delaying metamorphosis of bivalve larvae and application method thereof Active CN115053851B (en)

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JP2023083540A JP7421851B2 (en) 2022-05-25 2023-05-20 Method for delaying metamorphosis of juvenile bivalves using a device

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