CN115039777B - Compound plant growth regulator containing OPDA and COS and application thereof - Google Patents

Compound plant growth regulator containing OPDA and COS and application thereof Download PDF

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CN115039777B
CN115039777B CN202210852612.8A CN202210852612A CN115039777B CN 115039777 B CN115039777 B CN 115039777B CN 202210852612 A CN202210852612 A CN 202210852612A CN 115039777 B CN115039777 B CN 115039777B
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growth regulator
plant growth
opda
cos
compound
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CN115039777A (en
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薛娴
石欣隆
侯小改
杨月琴
刘伟
张利霞
张梦圆
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Henan University of Science and Technology
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/42Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/20Cereals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/20Cereals
    • A01G22/22Rice
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/40Fabaceae, e.g. beans or peas
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00Plant growth regulators
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Plant Pathology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to a compound plant growth regulator containing OPDA and COS and application thereof, belonging to the field of crops, wherein the active ingredients are 12-oxo-plant-dienoic acid (OPDA) and Chitosan Oligosaccharide (COS), the weight percentage of the OPDA in the compound plant growth regulator is 0.0005% -0.0035%, and the weight percentage of the COS in the compound plant growth regulator is 0.005% -0.025%; the compound plant growth regulator can be processed into a liquid preparation, wherein the liquid preparation can be any agricultural preparation, and is preferably water aqua, microemulsion, water dispersible granule or suspending agent; the invention has good promotion and yield increase effects on rice, wheat and peanut.

Description

Compound plant growth regulator containing OPDA and COS and application thereof
Technical Field
The invention belongs to the field of crops, and particularly relates to a compound plant growth regulator containing 12-oxo-plant dienoic acid (OPDA) and Chitosan (COS) and application thereof.
Background
The 12-Oxo Plant Dienoic Acid (OPDA) is an important signal molecule in plants, is related to plant stress reaction and natural immunity, can promote plants to generate various defense reactions such as allergic reaction, systemic acquired resistance and the like, induces stress gene expression, promotes the synthesis of related proteins and nucleic acid, activates the autoimmune function of the plants, and plays an important role in mediating the plant resistance reaction to biological stresses such as pathogen infection, insect bite and the like and various abiotic stresses such as low temperature, drought, waterlogging, phytotoxicity and the like.
OPDA is also used as a plant growth regulator to induce the synthesis of various antibacterial enzymes such as Plant Polyphenol Oxidase (PPO), protease Inhibitor (PI), lipoxygenase (LOX), peroxidase (POD) and Phenylalanine Ammonia Lyase (PAL), and to improve the activity of the related enzymes and enhance the stress resistance of plants; can also regulate and control the content of in vivo endogenous hormones, promote cell division, delay leaf aging, prolong green-keeping time, increase chlorophyll content and improve photosynthetic efficiency.
Chitosan Oligosaccharide (COS) is a safe and environment-friendly biological hormone, can remarkably improve the yield and quality of agricultural products, and has very wide application in the agricultural field. COS can activate the innate immune system of plants, stimulate the plants to generate immune response, combine with receptors on plant cells, and excite the expression of resistance genes, thereby enhancing the disease resistance of the plants. COS does not directly inactivate viruses, but has a remarkable inhibiting effect on viral replication and long-distance movement in plants. The chitosan oligosaccharide can also improve rhizosphere microorganism population, inhibit nematode growth, stimulate plant rooting, promote plant growth in early stage, shorten nutrient growth time, prolong reproductive growth time, and lay a foundation for increasing yield.
The reasonable and advanced compound formula not only can improve the stress resistance of plants, but also can promote the nutrition distribution in the growth and development of plants to develop towards the direction required by human beings, which is also the key for ensuring the yield and quality of plants. The prior art formulation field has not been reported about the preparation of a compound plant growth regulator from 12-oxo-plant-dienoic acid (OPDA) and Chitosan Oligosaccharide (COS).
Disclosure of Invention
In view of the above problems, the present invention aims at providing a compound plant growth regulator containing 12-oxo-plant dienoic acid (OPDA) and Chitosan Oligosaccharide (COS), at providing a method for promoting crop growth and increasing yield, and at providing an application of the plant growth regulator in crop growth regulation. The effective components of the plant growth regulator comprise 12-Oxo Plant Dienoic Acid (OPDA) and Chitosan Oligosaccharide (COS), can be used for regulating the growth of crops such as rice, wheat, peanut and the like, and has excellent promoting and yield increasing effects.
The invention adopts the following specific scheme:
the first aspect of the present invention provides a compound plant growth regulator comprising 12-oxo-plant dienoic acid and chitosan; the weight percentage of the 12-oxo plant dienoic acid in the compound plant growth regulator is 0.0005-0.0035 percent, and the weight percentage of the chitosan in the compound plant growth regulator is 0.005-0.025 percent.
As a further optimization of the compound plant growth regulator, the weight percentage of the 12-oxo plant dienoic acid in the compound plant growth regulator is 0.0015-0.0020 percent, and the weight percentage of the chitosan in the compound plant growth regulator is 0.010-0.015 percent. Further, the weight percentage of the 12-oxo plant dienoic acid in the compound plant growth regulator is 0.0015%, and the weight percentage of the chitosan in the compound plant growth regulator is 0.010%.
As a further optimization of the compound plant growth regulator, the compound plant growth regulator is a liquid preparation, and the liquid preparation is prepared by uniformly mixing and processing the raw materials. Preferably, the liquid preparation is water aqua, microemulsion, water dispersible granule or suspending agent.
As a further optimization of the compound plant growth regulator, the compound plant growth regulator also comprises an auxiliary agent, wherein the auxiliary agent is a solvent, an emulsifying agent, a humectant, a safener, a stabilizer, a dispersing agent or a defoaming agent.
The second aspect of the invention provides a method for promoting crop growth and increasing yield, which adopts a pesticide application method to apply the compound plant growth regulator to crops. Preferably, the pesticide application method is a spraying method, a dusting method, a fumigation method or a soil treatment method.
The third aspect of the invention provides application of the compound plant growth regulator in promoting crop growth and increasing yield. Preferably, the crop is rice, wheat or peanut.
The beneficial effects are that: the compound plant growth regulator has excellent promotion effect on crops such as rice, wheat, peanut and the like, and the effect of the compound preparation is greatly improved compared with that of a single preparation by compounding 12-oxo-plant dienoic acid (OPDA) and Chitosan Oligosaccharide (COS) according to a certain proportion, so that the compound preparation has great development value compared with that of a single preparation.
Detailed Description
The invention provides a compound plant growth regulator containing 12-oxo-plant dienoic acid and chitosan oligosaccharide, wherein the effective components comprise 12-oxo-plant dienoic acid (OPDA) and Chitosan Oligosaccharide (COS), the weight percentage of the OPDA in the compound plant growth regulator is 0.0005-0.0035%, the weight percentage of the COS in the compound plant growth regulator is 0.005-0.025%, and the rest components are any auxiliary agent commonly used in the agricultural field.
Preferably, the weight percentage of OPDA in the compound plant growth regulator is 0.0015-0.0020%, and the weight percentage of COS in the compound plant growth regulator is 0.010-0.015%. More preferably, the weight percentage of OPDA in the compound plant growth regulator is 0.0015%, and the weight percentage of COS in the compound plant growth regulator is 0.010%.
The compound plant growth regulator can be prepared into any preparation used in agriculture, including but not limited to water aqua, microemulsion, water dispersible granule or suspending agent, preferably water aqua.
The auxiliary agents used in the compound plant growth regulator are all various auxiliary agents commonly used in pesticide preparation processing, and include but are not limited to solvents, emulsifying agents, humectants, safeners, stabilizers, dispersants and defoamers.
The production process of the compound plant growth regulator of the invention belongs to the prior known technology and is not described in detail here.
The mode of applying the composition of the compound plant growth regulator of the invention is any effective conventional method in pesticide application, including but not limited to spraying, dusting, fumigation, soil treatment and the like. Preferably, a spray method is used.
The compound plant growth regulator is mainly used for regulating the growth of crops such as rice, wheat, peanut and the like.
The technical scheme of the invention will be clearly and completely described in the following in connection with the embodiments of the invention.
In the examples, 12-oxo-plant-dienoic acid (OPDA) and Chitosan Oligosaccharide (COS) were purchased from Shanghai Ming Biotech Co. The specific conditions are not specified in the examples and are carried out under conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.
Example 1: the indoor potting test is carried out, and the steps are as follows:
the 12-Oxo Plant Dienoic Acid (OPDA) and the Chitosan Oligosaccharide (COS) are compounded according to a certain proportion, and the solvent is water aqua. The potted rice is tested, and the composite agents with different proportions and different contents and single agent are used for respectively carrying out blade spray treatment at the tillering stage and the booting stage of the rice until water drops on the upper surface and the lower surface of the blade. The effect is measured by measuring the agronomic characters such as average spike length, thousand grain weight, yield increase and the like of the crop in the later period and blank control. The test results are shown in tables 1-3.
Table 1: influence of 12-Oxo Plant Dienoic Acid (OPDA) on the yield of potted rice.
OPDA ratio% Average spike length (cm) Thousand grain weight (g) Yield increase%
0/CK 5.7 23.4 ——
0.0005 5.9 24.1 2.99
0.0010 6.0 24.9 6.41
0.0015 6.5 26.1 11.54
0.0020 6.5 26.1 11.54
0.0025 6.3 25.8 10.26
0.0030 6.3 25.2 7.69
0.0035 6.2 24.8 5.98
Table 2: influence of Chitosan Oligosaccharide (COS) on the yield of potted rice.
Table 3: influence of the 12-oxo-plant-dienoic acid (OPDA) and Chitosan Oligosaccharide (COS) formulations on the yield of potted rice.
Compounding proportion% Average spike length (cm) Thousand grain weight (g) Yield increase%
0/CK 5.7 23.4 ——
OPDA:COS(0.0015:0.010) 7.1 27.3 16.67
OPDA:COS(0.0015:0.015) 7.0 27.1 15.81
OPDA:COS(0.0020:0.010) 6.9 27.0 15.38
OPDA:COS(0.0020:0.015) 6.8 26.9 14.96
The indoor potted rice measurement yield test shows that the rice yield is increased by spraying the compound agent of the 12-oxo-plant dienoic acid (OPDA) and the Chitosan Oligosaccharide (COS) with proper concentration; the compound agent has obviously better yield-increasing effect than single agent, and each agronomic character is better than that of blank control. Preferably, the weight percentage of OPDA in the compound plant growth regulator is 0.0015-0.0025%, and the weight percentage of COS in the compound plant growth regulator is 0.010-0.015%. More preferably, the weight percentage of OPDA in the compound plant growth regulator is 0.0015%, and the weight percentage of COS in the compound plant growth regulator is 0.010%. The process was performed in a laboratory with stringent conditions, and the actual production field conditions were complex and widely varied, so that verification in the field was also required, and example 2 was performed.
Example 2: the rice field efficacy test comprises the following steps:
the preparation method comprises the steps of carrying out leaf spraying treatment on rice in the tillering stage and the booting stage respectively by using a compound preparation or a single agent containing 12-oxo-plant dienoic acid (OPDA) and Chitosan Oligosaccharide (COS) in different proportions and different contents, wherein a solvent is an aqueous agent, so as to achieve water dripping on the upper surface and the lower surface of the leaf. The effect is measured by measuring the acre yield, yield increase and other characters of the crop in the later period and the blank control. The test results are shown in Table 4.
Table 4: influence of 12-oxo-plant-dienoic acid (OPDA) and Chitosan Oligosaccharide (COS) formulations on rice yield.
The rice yield test in the field shows that the rice yield is increased by spraying the compound agent of 12-oxo-plant dienoic acid (OPDA) and Chitosan Oligosaccharide (COS) with proper concentration; the yield increasing effect of the compound agent is obviously better than that of a single agent. Preferably, the weight percentage of OPDA in the compound plant growth regulator is 0.0015-0.0025%, and the weight percentage of COS in the compound plant growth regulator is 0.010-0.015%. More preferably, the weight percentage of OPDA in the compound plant growth regulator is 0.0015%, and the weight percentage of COS in the compound plant growth regulator is 0.010%.
Example 3: the indoor potting test is carried out, and the steps are as follows:
example 3 was performed on the basis of example 1. The method comprises the steps of compounding 12-oxo-plant dienoic acid (OPDA) and Chitosan Oligosaccharide (COS) according to a certain proportion, taking a water aqua as a solvent, carrying out an indoor potting test on wheat, respectively spraying the solution on the wheat for 1 time in a booting period and a grouting period, and measuring the effect of the wheat by measuring the agronomic characters such as average spike length, thousand kernel weight, yield increase and the like of the crop later period and a blank control. The test results are shown in tables 5-7.
Table 5: effect of 12-Oxo Plant Dienoic Acid (OPDA) on potted wheat yield.
OPDA ratio% Average spike length (cm) Thousand grain weight (g) Yield increase%
0/CK 4.7 32.4 ——
0.0005 5.0 33.3 2.78
0.0010 5.2 34.2 5.56
0.0015 5.6 36.2 11.73
0.0020 5.6 36.1 11.42
0.0025 5.5 35.4 9.26
0.0030 5.4 35.2 8.64
0.0035 5.4 34.9 7.72
Table 6: effect of Chitosan Oligosaccharide (COS) on potted wheat yield.
COS proportion% Average spike length (cm) Thousand grain weight (g) Yield increase%
0/CK 4.7 32.4 ——
0.005 5.1 33.8 4.32
0.010 5.7 36.6 12.96
0.015 5.6 36.3 12.03
0.020 5.3 35.3 8.95
0.025 5.2 34.7 7.10
Table 7: influence of 12-oxo-plant-dienoic acid (OPDA) and Chitosan Oligosaccharide (COS) formulations on potted wheat yield.
Compounding proportion% Average spike length (cm) Thousand grain weight (g) Yield increase%
0/CK 4.7 32.4 ——
OPDA:COS(0.0015:0.010) 5.9 37.9 16.98
OPDA:COS(0.0015:0.015) 5.8 37.1 14.51
OPDA:COS(0.0020:0.010) 5.8 36.8 13.58
OPDA:COS(0.0020:0.015) 5.8 36.7 13.27
The indoor potted rice measurement yield test shows that the rice yield is increased by spraying the compound agent of the 12-oxo-plant dienoic acid (OPDA) and the Chitosan Oligosaccharide (COS) with proper concentration; the compound agent has obviously better yield-increasing effect than single agent, and each agronomic character is better than that of blank control. Preferably, the weight percentage of OPDA in the compound plant growth regulator is 0.0015-0.0025%, and the weight percentage of COS in the compound plant growth regulator is 0.010-0.015%. More preferably, the weight percentage of OPDA in the compound plant growth regulator is 0.0015%, and the weight percentage of COS in the compound plant growth regulator is 0.010%. The process was performed in a laboratory with stringent conditions, and the actual production field conditions were complex and widely varied, so that verification in the field was also required, and example 4 was performed.
Example 4: the wheat field efficacy test comprises the following steps:
example 4 was performed on the basis of example 2 and example 3. The method comprises the steps of compounding 12-oxo-plant dienoic acid (OPDA) and Chitosan Oligosaccharide (COS) according to a certain proportion, taking a water agent as a solvent, carrying out field efficacy test on wheat, respectively spraying the water agent on wheat for 1 time in the booting period and the grouting period, treating crops by using complexing agents with different proportions and different contents and single agent, and measuring the effect of the crops by measuring the acre yield, the yield increase and other characters of the later period and blank contrast of the crops. The test results are shown in Table 8.
Table 8: influence of 12-oxo-plant-dienoic acid (OPDA) and Chitosan Oligosaccharide (COS) formulations on wheat yield.
Compounding proportion% Wheat acre yield (kg) Yield increase%
0/CK 401.4 ——
OPDA:COS(0.000:0.010) 433.2 7.92
OPDA:COS(0.000:0.015) 427.4 6.48
OPDA:COS(0.0015:0.000) 438.6 9.27
OPDA:COS(0.0015:0.010) 476.2 18.63
OPDA:COS(0.0015:0.015) 451.6 12.51
OPDA:COS(0.0020:0.000) 434.4 8.22
OPDA:COS(0.0020:0.010) 449.2 11.91
OPDA:COS(0.0020:0.015) 446.0 11.11
As can be seen from Table 8, the yield increase effect of the compound agent of 12-oxo-plant dienoic acid (OPDA) and Chitosan Oligosaccharide (COS) is obviously better than that of a single agent through the field wheat measurement yield test. Preferably, the weight percentage of OPDA in the compound plant growth regulator is 0.0015-0.0025%, and the weight percentage of COS in the compound plant growth regulator is 0.010-0.015%. More preferably, the weight percentage of OPDA in the compound plant growth regulator is 0.0015%, and the weight percentage of COS in the compound plant growth regulator is 0.010%. Indoor and field comparison experiments show that the compound preparation of the 12-oxo-plant dienoic acid (OPDA) and the Chitosan Oligosaccharide (COS) has a good yield increasing effect, and is worthy of popularization.
In the embodiment of the invention, the sowing areas are all in Henan.
It should be noted that the above-mentioned embodiments are to be understood as illustrative, and not limiting, the scope of the invention, which is defined by the appended claims. It will be apparent to those skilled in the art that various modifications and adaptations can be made to the present invention without departing from its spirit or scope.

Claims (9)

1. A compound plant growth regulator, which is characterized in that: the compound plant growth regulator comprises 12-oxo plant dienoic acid and chitosan oligosaccharide; the weight percentage of the 12-oxo plant dienoic acid in the compound plant growth regulator is 0.0015% -0.0020%, and the weight percentage of the chitosan oligosaccharide in the compound plant growth regulator is 0.010% -0.015%.
2. The built plant growth regulator according to claim 1, wherein: the weight percentage of the 12-oxo plant dienoic acid in the compound plant growth regulator is 0.0015%, and the weight percentage of the chitosan oligosaccharide in the compound plant growth regulator is 0.010%.
3. The built plant growth regulator according to claim 1, wherein: the compound plant growth regulator is a liquid preparation, and the liquid preparation is prepared by uniformly mixing and processing the raw materials.
4. A built plant growth regulator according to claim 3, wherein: the liquid preparation is water aqua, microemulsion, water dispersible granule or suspending agent.
5. The built plant growth regulator according to claim 1, wherein: the compound plant growth regulator also comprises an auxiliary agent, wherein the auxiliary agent is a solvent, an emulsifying agent, a humectant, a safener, a stabilizer, a dispersing agent or a defoaming agent.
6. A method for promoting crop growth and increasing yield, which is characterized in that: applying the compound plant growth regulator according to any one of claims 1 to 5 to crops by a pesticide application method.
7. The method according to claim 6, wherein: the pesticide application method is a spraying method, a powder spraying method, a fumigation method or a soil treatment method.
8. Use of the compound plant growth regulator according to any one of claims 1 to 5 for promoting crop growth and increasing yield.
9. The use according to claim 8, characterized in that: the crop is rice, wheat or peanut.
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壳寡糖对烟草悬浮细胞茉莉酸合成基因转录的影响;王文霞;李曙光;赵小明;林炳承;杜昱光;;植物学通报(第05期);全文 *
茉莉素、生长素和表观遗传调控水稻颖花发育的研究进展;陈睿;福建农业科技(第1期);第62-67页 *

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