CN115024412A

CN115024412A - Functional compound for promoting growth of piglets and preparation method and application thereof

Info

Publication number: CN115024412A
Application number: CN202210672320.6A
Authority: CN
Inventors: 谢福生; 杜仲海
Original assignee: Guangzhou Pucheng Agriculture And Animal Husbandry Technology Co ltd
Current assignee: Guangzhou Pucheng Agriculture And Animal Husbandry Technology Co ltd
Priority date: 2022-06-14
Filing date: 2022-06-14
Publication date: 2022-09-09

Abstract

The invention provides a functional compound for promoting piglet growth and a preparation method and application thereof, wherein the functional compound comprises 30-50 parts of piglet structural fat, 1-10 parts of inulin short-chain fatty acid ester, 1-10 parts of polysaccharide compound, 1-5 parts of glycine iron and 1-5 parts of phytase by weight; the raw materials for preparing the piglet structure fat comprise 1, 3-dioleic acid-2-medium chain fatty acid triglyceride, coix seed oil and chili oil. The functional compound provided by the invention can promote the growth and development of intestinal villi of piglets, maintain the health of intestinal tracts, improve the digestion and absorption capacity of the piglets on nutrient substances, reduce diarrhea, avoid the anemia problem of the piglets and effectively promote the growth of the piglets.

Description

Functional compound for promoting growth of piglets and preparation method and application thereof

Technical Field

The invention belongs to the technical field of feeds, and particularly relates to a functional compound for promoting piglet growth, a preparation method and an application thereof, in particular to a functional compound for promoting piglet growth and capable of promoting piglet intestinal villus development, and a preparation method and an application thereof.

Background

Due to the continuous progress of modern breeding technology, the weaning days of piglets are gradually advanced. The daily ration of the weaned piglets is changed from breast milk to solid or liquid feed, but the digestive system development of the piglets is not complete, the structure of the exogenous feed is different from that of the breast milk, and the exogenous feed is not easy to be digested and absorbed, so that the dyspepsia, the immunity and the disease resistance of the piglets are reduced, even the diarrhea is caused, and the great economic loss is brought to the pig raising production.

The intestinal tract is not only the digestive organs of animals, but also the largest endocrine organs in the body. Before and after the pigs are weaned, the structure and the function of the intestinal tract are greatly changed, and the stress can cause the internal organs and tissues of the piglets to be pathologically damaged, the intestinal villus is atrophied, the indigestion and malabsorption are caused, and the inflammation is caused.

CN108902515A discloses a feed premix for increasing piglet intestinal villus height and reducing crypt depth, and a preparation method and application thereof, wherein each 100 parts by weight of the feed premix comprises 20-40 parts by weight of silage straw, 15-35 parts by weight of intestinal membrane protein, 10-25 parts by weight of citric acid, 10-20 parts by weight of glucose, 4-9 parts by weight of oregano extract, 5-10 parts by weight of beer yeast, 2-5 parts by weight of salt, and the balance of carrier. The effective viable bacteria content in per g of cerevisiae Fermentum is 7 × 10 ⁹ -7.5×10 ⁹ And (4) CFU. The feed premix does not generate drug resistance, and the raw material components are matched and used with good and stable effect. The preparation method mixes the raw materials according to the proportion, is simple and quick, and has good feed stability. The feed premix is added into a weaned piglet feed to improve the intestinal function of piglets, regulate the intestinal health, enhance the immunity and effectively improve the growth performance of the piglets.

CN106135691A discloses a feed additive for promoting intestinal health of piglets, which comprises the following raw materials in parts by weight: 10-15 parts of yeast fermentation culture, 8-12 parts of coix seed, 10-15 parts of composite plant extract, 6-8 parts of galangal and 12-16 parts of fingered citron. The additive is applied to piglets, and can promote intestinal villus development of piglets, increase beneficial bacteria flora quantity in intestinal tract, improve intestinal mucosa immunity, improve piglet growth performance, reduce diarrhea rate, and significantly improve breeding benefit. Meanwhile, the feed additive also has the function of replacing antibiotics, and has important significance for promoting the development of live pig breeding industry towards green and safe direction.

Due to the fact that the requirements for nutrients of the piglets after weaning are high, the conventional feed is often lack of the important functional nutrients, and therefore intestinal tracts of the piglets are damaged and grow slowly. Therefore, how to provide a functional compound which can rapidly provide energy and high-quality nutrition for the intestinal tracts of piglets, relieve the damage of the intestinal barrier function, repair the intestinal villus structure and promote the growth of the intestinal villus of the piglets, thereby improving the digestion and utilization rate of the piglets on nutrient substances and promoting the rapid growth of the piglets becomes a problem to be solved urgently.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide a functional compound for promoting the growth of piglets and a preparation method and application thereof, and particularly provides a functional compound for promoting the growth of piglets, which can promote the development of intestinal villi of the piglets, and a preparation method and application thereof. The functional compound provided by the invention can effectively promote the growth and development of intestinal villi of piglets, maintain the intestinal health of piglets, reduce the diarrhea condition, improve the digestion and absorption capacity of the piglets on nutrient substances, and avoid the anemia problem of the piglets, thereby promoting the rapid growth of the piglets.

In order to achieve the purpose, the invention adopts the following technical scheme:

according to the first aspect, the invention provides a functional compound for promoting growth of piglets, which comprises 30-50 parts of piglet structural fat, 1-10 parts of inulin short-chain fatty acid ester, 1-10 parts of polysaccharide compound, 1-5 parts of glycine iron and 1-5 parts of phytase by weight.

The preparation raw materials of the piglet structure fat comprise 1, 3-dioleic acid-2-medium chain fatty acid triglyceride (OMO type structure ester), coix seed oil and chili oil.

The piglet structural fat can be 30 parts, 35 parts, 40 parts, 45 parts or 50 parts, the inulin short-chain fatty acid ester can be 1 part, 3 parts, 5 parts, 7 parts, 9 parts or 10 parts, the polysaccharide complex can be 1 part, 3 parts, 5 parts, 7 parts, 9 parts or 10 parts, the glycine iron can be 1 part, 2 parts, 3 parts, 4 parts or 5 parts, the phytase can be 1 part, 2 parts, 3 parts, 4 parts or 5 parts, but the method is not limited to the above-mentioned numerical values, and other numerical values in the above-mentioned numerical value range can be also applied.

The functional compound is compounded by adopting three compounds of piglet structural fat, inulin short-chain fatty acid ester and polysaccharide compound, and the synergistic effect can effectively promote the growth and development of piglet intestinal villi, maintain the piglet intestinal health, improve the digestion and absorption capacity of piglets on nutrient substances, promote the piglet growth and reduce the diarrhea condition; the iron glycinate and the phytase are compounded, so that the anemia problem of piglets can be avoided, and the apoptosis of intestinal cells can be reduced, so that the intestinal barrier function damage is relieved; and the 1, 3-dioleic acid-2-medium chain fatty acid triglyceride, the coix seed oil and the chili oil are compounded, so that the effect of the piglet structure fat can be further improved.

The OMO-type structural ester is a symmetrical medium-long chain triglyceride containing oleic acid at the sn-1 and 3 positions and medium-chain fatty acids at the sn-2 position, which is helpful for absorption of nutrients, and the medium-chain fatty acids are more easily digested and metabolized in the body than the long-chain fatty acids. After entering human body, the sn-1, 3-position oleic acid is hydrolyzed, the medium chain fatty acid in the form of sn-2 monoglyceride can be directly absorbed without being re-esterified into triglyceride, and has the effect of fast energy supply compared with the common medium chain fatty acid ester; while medium and long chain triglycerides differ in their metabolic pathways in the body. Long chain triglycerides are broken down and transported to mucosal cells via lymph to form new triglycerides. While medium chain triglycerides are hydrolyzed by lipases to medium chain fatty acids, which enter the liver via the portal vein and are oxidized to H in the liver ₂ O、CO ₂ And energy or carcass formation, not readily storable in adipocytes or liver tissue; the OMO type structural ester combines the two advantages, and has the effects of rapidly supplying energy and promoting piglet production.

Preferably, the raw materials for preparing the piglet structure fat comprise 60-80 parts of 1, 3-dioleic acid-2-medium chain fatty acid triglyceride, 1-5 parts of coix seed oil and 1-10 parts of chili oil in parts by weight.

The number of parts of the 1, 3-dioleoyl-2-medium-chain fatty acid triglyceride may be 60 parts, 65 parts, 70 parts, 75 parts, or 80 parts, the number of parts of the coix seed oil may be 1 part, 2 parts, 3 parts, 4 parts, or 5 parts, and the number of parts of the capsicum oil may be 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, or 10 parts, but is not limited to the above-mentioned values, and other values not listed in the above-mentioned numerical range may be similarly applied.

Preferably, the raw materials for preparing the piglet structure fat also comprise a carrier.

Preferably, the piglet structural fat is prepared by a method comprising the following steps: heating and dissolving 1, 3-dioleic acid-2-medium chain fatty acid triglyceride, coix seed oil and chili oil, mixing with carrier, spraying, and drying to obtain the piglet structure fat.

Preferably, the 1, 3-dioleoyl-2-medium chain fatty acid triglycerides include 1, 3-dioleoyl-2-caprylic triglyceride, 1, 3-dioleoyl-2-capric triglyceride, 1, 3-dioleoyl-2-lauric triglyceride, and 1, 3-dioleoyl-2-myristic triglyceride.

Preferably, the mass ratio of the 1, 3-dioleic-2-caprylic triglyceride, 1, 3-dioleic-2-capric triglyceride, 1, 3-dioleic-2-lauric triglyceride and 1, 3-dioleic-2-myristic triglyceride is (1-3): 1-5): 1-2): 3-5, wherein the part of the 1, 3-dioleic-2-caprylic triglyceride can be 1, 2 or 3, etc., the part of the 1, 3-dioleic-2-capric triglyceride can be 1, 2, 3, 4 or 5, etc., the part of the 1, 3-dioleic-2-lauric triglyceride can be 1, 1.5 or 2, etc., the part of the 1, 3-dioleic-2-myristic triglyceride can be 3, 3, 4, 5, etc., but are not limited to the numerical values recited above, and other numerical values not recited within the above numerical ranges are also applicable.

The 1, 3-dioleoyl-2-medium chain fatty acid triglyceride is compounded by adopting specific four components, and the synergistic effect can effectively improve the effect of the 1, 3-dioleoyl-2-medium chain fatty acid triglyceride.

Preferably, the carrier includes any one or a combination of at least two of casein, lactose or soy protein isolate, such as a combination of casein and lactose, a combination of lactose and soy protein isolate or a combination of casein and soy protein isolate, etc., but is not limited to the above-listed combinations, and other combinations not listed within the above-mentioned combinations are equally applicable.

Preferably, the carrier is a combination of casein, lactose, soy protein isolate.

Preferably, the mass ratio of casein, lactose and soy protein isolate is (1-3): 1-2):1, for example 1:1:1, 2:2:1 or 3:1:1, but not limited to the above-listed values, and other values not listed in the above-mentioned range of values are equally applicable.

Preferably, the mass ratio of the total mass of the 1, 3-dioleoyl-2-medium chain fatty acid triglyceride, the coix seed oil and the chilli oil to the carrier is (2-3) to (2-3), for example, 2:3, 2.5:2.5 or 3:2, but is not limited to the above-listed values, and other values not listed in the above-mentioned value range are also applicable.

Preferably, the inulin short-chain fatty acid ester includes inulin acetate, inulin propionate and inulin butyrate ester.

Preferably, the mass ratio of the inulin acetate, the inulin propionate and the inulin butyrate is (9-15): (5-8): (3-5), wherein the part of the inulin acetate can be 9, 10, 11, 12, 13, 14 or 15, etc., the part of the inulin propionate can be 5, 6, 7 or 8, etc., and the part of the inulin butyrate can be 3, 4 or 5, etc., but is not limited to the above-listed values, and other values not listed in the above-mentioned numerical range are also applicable.

The inulin short-chain fatty acid ester is compounded by inulin acetate, inulin propionate and inulin butyrate, and the effect of the inulin short-chain fatty acid ester can be further improved under the synergistic effect.

The inulin short-chain fatty acid is not easy to digest in oral cavity and stomach small intestine, and is decomposed into inulin and short-chain fatty acid after reaching the small intestine, the short-chain fatty acid can provide energy for small intestine epithelium to promote intestinal villus development, and the inulin is fermented under the action of a large amount of beneficial bacteria after reaching colon to generate SCFA.

The inulin short-chain fatty acid supplement not only improves the defect of directly supplementing short-chain fatty acid (SCFA), conveys the SCFA to the intestinal tract to promote the growth and development of intestinal villi of piglets, but also provides high-quality fiber for piglets, promotes the proliferation of intestinal probiotics such as bifidobacterium, lactobacillus and the like, and improves the intestinal flora, promotes the growth and development of intestinal villi of piglets, mineral absorption and the like by virtue of the SCFA produced by fermentation under the action of intestinal microorganisms.

Preferably, the inulin short-chain fatty acid ester has an average degree of polymerization of not more than 5, such as 5, 4, 3 or 2, etc., but not limited to the above-listed values, and other values not listed within the above-mentioned range of values are equally applicable.

Preferably, the polysaccharide complex comprises hericium erinaceus polysaccharide, lentinan, rosa roxburghii polysaccharide and alfalfa polysaccharide.

Preferably, the ratio of the hericium erinaceus polysaccharide to the lentinan to the rosa roxburghii polysaccharide to the alfalfa polysaccharide is (3-5): (1-2): (3-7): (2-4), wherein the part of the hericium erinaceus polysaccharide can be 3, 4 or 5, etc., the part of the lentinan can be 1, 1.5 or 2, etc., the part of the rosa roxburghii polysaccharide can be 3, 4, 5, 6 or 7, etc., and the part of the alfalfa polysaccharide can be 2, 3 or 4, etc., but is not limited to the above-listed values, and other values not listed in the above-mentioned range of values are also applicable.

The polysaccharide compound is prepared by compounding hericium erinaceus polysaccharide, lentinan, rosa roxburghii tratt polysaccharide and alfalfa polysaccharide, so that the effect of the polysaccharide compound can be further improved; can be used as prebiotics in intestinal tract to stimulate intestinal tract mucosa immune system to make immune response, and can interact with intestinal tract microorganism to improve intestinal tract environment and intestinal tract flora structure. Can also promote the growth of intestinal villi and maintain the integrity and balance of mucous membranes of intestinal flora, thereby effectively reducing the incidence of diseases.

In a second aspect, the present invention provides a method for preparing a functional complex as described above, comprising the steps of:

and mixing piglet structural fat, inulin short-chain fatty acid ester, polysaccharide complex, glycine iron and phytase to obtain the functional complex.

In a third aspect, the invention also provides an application of the functional compound in preparing piglet feed.

Compared with the prior art, the invention has the following beneficial effects:

the invention provides a functional compound for promoting the growth of piglets, which can effectively promote the growth and development of intestinal villi of the piglets, maintain the intestinal health of the piglets, improve the digestion and absorption capacity of the piglets on nutrient substances, promote the growth of the piglets and reduce the diarrhea condition by adopting the compounding of piglet structural fat, inulin short-chain fatty acid ester and polysaccharide compound and the synergistic action; and the compound of the glycine iron and the phytase effectively improves the iron absorption rate of piglets, avoids anemia of the piglets, protects intestinal cells, reduces the apoptosis of the intestinal cells, thereby alleviating the damage of the intestinal barrier function, further maintaining the intestinal health of the piglets and promoting the rapid growth of the piglets.

Detailed Description

To further illustrate the technical means and effects of the present invention, the following preferred embodiments of the present invention are further described, but the present invention is not limited to the scope of the embodiments.

In the following examples, coix seed oil was purchased from shanghai brocade toona sinensis biotechnology limited;

the chili oil is purchased from Shanghai brocade toona sinensis Biotech Co., Ltd;

soy protein isolate was purchased from heinan old shellfish biotechnology limited;

inulin acetate was purchased from gardley biochemical technologies, ltd, guangzhou;

inulin propionate was purchased from Guangzhou Jiadeli Biochemical technology, Inc.;

inulin butyrate was purchased from gardley biochemical technologies, ltd, guangzhou;

hericium erinaceus polysaccharides were purchased from Hibiscus biosciences, Inc;

lentinan was purchased from Hibiscus Shinit Biotech, Inc.;

rosa roxburghii polysaccharide is purchased from Hibiscus parnaschii Biotech Co., Ltd;

alfalfa polysaccharide was purchased from downy snout biotechnology limited;

phytase was purchased from Hibiscus Sainus Biotech, Inc.

The parts are in the following examples by weight.

Preparation example 1

The preparation example provides a piglet structure fat, and the preparation method comprises the following steps:

the piglet structure fat is obtained by fully mixing 14 parts of 1, 3-dioleic acid-2-caprylic triglyceride, 21 parts of 1, 3-dioleic acid-2-capric triglyceride, 7 parts of 1, 3-dioleic acid-2-lauric triglyceride, 28 parts of 1, 3-dioleic acid-2-myristic triglyceride, 3 parts of coix seed oil, 5 parts of chili oil, 26 parts of casein, 26 parts of lactose and 26 parts of isolated soy protein, and then carrying out spray drying.

Preparation example 2

This preparation provides a piglet structured fat, which is the same as in preparation example 1 except that 1, 3-dioleic acid-2-decanoic acid triglyceride is not included in the preparation, and a reduced portion is proportionally allocated to 1, 3-dioleic acid-2-octanoic acid triglyceride, 1, 3-dioleic acid-2-lauric acid triglyceride and 1, 3-dioleic acid-2-myristic acid triglyceride.

Preparation example 3

This preparation example provides a piglet structured fat, which was the same as in preparation example 1 except that 1, 3-dioleic-2-lauric triglyceride was not included and a reduced portion was proportionally allocated to 1, 3-dioleic-2-capric triglyceride, 1, 3-dioleic-2-caprylic triglyceride and 1, 3-dioleic-2-myristic triglyceride.

Preparation example 4

This preparation provides a piglet structured fat, which is identical to preparation example 1 except that 1, 3-dioleic acid-2-myristoleic acid triglyceride is not included in the preparation, and a reduced portion is proportionally allocated to 1, 3-dioleic acid-2-decanoic acid triglyceride, 1, 3-dioleic acid-2-lauric acid triglyceride and 1, 3-dioleic acid-2-caprylic acid triglyceride.

Preparation example 5

This preparation provides a piglet structured fat, which is the same as in preparation example 1 except that 1, 3-dioleate-2-caprylic triglyceride is not included in the preparation, and a reduced portion is proportionally distributed to 1, 3-dioleate-2-myristic triglyceride, 1, 3-dioleate-2-capric triglyceride and 1, 3-dioleate-2-lauric triglyceride.

Preparation example 6

The preparation example provides a piglet structure fat, and the preparation method is the same as the preparation example 1 except that the coix seed oil is not contained, and the part of the coix seed oil is reduced and distributed to the chili oil.

Preparation example 7

The preparation example provides a piglet structure fat, and the preparation method is the same as the preparation example 1 except that the piglet structure fat does not contain chili oil and the reduced part of the chili oil is distributed to the coix seed oil.

Preparation example 8

This preparation example provides a piglet structured fat, which is the same as preparation example 1 except that it does not contain 1, 3-dioleate-2-caprylic triglyceride, 1, 3-dioleate-2-myristic triglyceride, 1, 3-dioleate-2-capric triglyceride and 1, 3-dioleate-2-lauric triglyceride.

Example 1

This example provides a functional complex prepared from the following raw materials:

40 parts of piglet structure fat, 2.7 parts of inulin acetate, 1.4 parts of inulin propionate, 0.9 part of inulin butyrate, 1.5 parts of hericium erinaceus polysaccharide, 0.5 part of lentinan, 1.9 parts of rosa roxburghii tratt polysaccharide, 1.1 parts of alfalfa polysaccharide, 3 parts of ferric glycinate and 3 parts of phytase, which are provided by the preparation example 1.

The preparation method comprises the following steps:

and mixing the above raw materials to obtain the functional compound.

Example 2

50 parts of piglet structural fat provided by preparation example 1, 0.54 part of inulin acetate, 0.29 part of inulin propionate, 0.17 part of inulin butyrate, 3.33 parts of hericium erinaceus polysaccharide, 1.11 parts of lentinan, 3.33 parts of rosa roxburghii tratt polysaccharide, 2.23 parts of alfalfa polysaccharide, 5 parts of glycine iron and 1 part of phytase.

The preparation method is the same as example 1.

Example 3

30 parts of piglet structural fat, 5.3 parts of inulin acetate, 2.9 parts of inulin propionate, 1.8 parts of inulin butyrate, 0.27 part of hericium erinaceus polysaccharide, 0.11 part of lentinan, 0.39 part of rosa roxburghii tratt polysaccharide, 0.23 part of alfalfa polysaccharide, 1 part of glycine iron and 5 parts of phytase provided by preparation example 1.

The preparation method is the same as example 1.

Examples 4 to 10

Examples 4-13 each provide a functional complex, the starting materials for which are identical to those of example 1, except that the piglet structural fat provided in preparation example 1 is replaced with an equivalent amount of the piglet structural fat provided in preparation examples 2-8, respectively.

The preparation method is the same as example 1.

Example 11

This example provides a functional complex, which is identical to that of example 1, except that no inulin butyrate is added, and a reduced fraction is apportioned to inulin acetate, inulin propionate.

The preparation method is the same as example 1.

Example 12

This example provides a functional complex, which is identical to that of example 1, except that no inulin acetate is added, and a reduced fraction is apportioned to the butyrate and propionate inulin esters.

The preparation method is the same as example 1.

Example 13

This example provides a functional complex, which is identical to that of example 1, except that no alanate is added, and a reduced fraction is apportioned to alanate acetate and alanate butyrate.

The preparation method is the same as example 1.

Example 14

This example provides a functional complex, which is identical to example 1 except that no hericium erinaceus polysaccharide is added, and the reduced fraction is proportionally distributed to lentinan, rosa roxburghii tratt, alfalfa polysaccharide.

The preparation method is the same as example 1.

Example 15

The embodiment provides a functional compound, and the specific steps are the same as the embodiment 1 except that lentinan is not added, and the reduced part is proportionally distributed to hericium erinaceus polysaccharide, rosa roxburghii tratt polysaccharide and alfalfa polysaccharide.

The preparation method is the same as example 1.

Example 16

The embodiment provides a functional compound, and the specific steps are the same as the embodiment 1 except that no rosa roxburghii polysaccharide is added, and the reduced parts are proportionally distributed to lentinan, hericium erinaceus polysaccharide and alfalfa polysaccharide.

The preparation method is the same as example 1.

Example 17

The embodiment provides a functional compound, and the specific steps are the same as those of the embodiment 1 except that alfalfa polysaccharide is not added, and the reduced part is proportionally distributed to lentinan, rosa roxburghii tratt polysaccharide and hericium erinaceus polysaccharide.

The preparation method is the same as example 1.

Comparative example 1

This comparative example provides a functional complex, which was identical to that of example 1 except that no piglet structural lipids provided in preparation example 1 were added, and the reduced fractions were proportionally distributed to inulin acetate, inulin propionate, inulin butyrate, simian polysaccharide, lentinan, rosa roxburghii polysaccharide, alfalfa polysaccharide.

The preparation method is the same as example 1.

Comparative example 2

The comparative example provides a functional complex, which is identical to example 1 except that inulin acetate, inulin propionate and inulin butyrate are not added in the specific steps, and a reduced part is proportionally distributed to piglet structure fat, simian cephem polysaccharide, lentinan, roxburgh rose polysaccharide and alfalfa polysaccharide provided in preparation example 1.

The preparation method is the same as example 1.

Comparative example 3

The comparative example provides a functional complex, and the specific steps are the same as those in example 1 except that hericium erinaceus polysaccharide, lentinan, rosa roxburghii tratt polysaccharide and alfalfa polysaccharide are not added, and the reduced part is proportionally distributed to inulin acetate, inulin propionate and inulin butyrate and the piglet structure fat provided in preparation example 1.

The preparation method is the same as example 1.

And (3) effect testing:

567 healthy and 7 kg-weight Du-Dai three-way hybrid weaned pigs were selected, randomly and evenly divided into 21 groups, each group had 3 replicates, 9 replicates each, and fed with commercial piglet feed mix (Aaonong Biotech Co., Ltd., Guangzhou, control group) and 5% of the functional compound provided in examples 1-17 and comparative examples 1-3, respectively, on the basis of the commercial piglet feed mix, the test period was 49 days, and 1 piglet with the weight closest to the average weight of the group was selected and labeled for each group, and was slaughtered in order. Collecting tissues of duodenum (1cm), jejunum (3cm) and ileum (2cm), washing with normal saline, and fixing in 4% paraformaldehyde solution; collecting duodenal mucosa and jejunum mucosa samples and colon and caecum chyme samples, quickly freezing by using liquid nitrogen, and storing in a refrigerator at the temperature of minus 80 ℃ to be tested.

Intestinal tract morphological structure

Samples of duodenum, jejunum and ileum fixed by 4% paraformaldehyde are taken out to be prepared into paraffin sections, then hematoxylin-eosin (HE) staining is carried out, Image acquisition is carried out under a microscope after dehydration and mounting, the height of villus and the depth of crypt of each intestinal section are measured by using Image-pro plus 6.0 software and taking a 40-fold scale as a standard, and the height/depth of the villus are calculated.

The daily gain, feed ratio, diarrhea rate and intestinal tract morphological structure of piglets are recorded, and the average value is calculated, and the result is as follows:

compared with the control group, the product provided by the invention can effectively promote the growth and development of intestinal villi of piglets, improve the digestion and absorption capacity of the piglets on feed, promote the growth of the piglets and reduce the diarrhea condition; comparing examples 1 and 4-7, the invention can find that the efficacy of the OMO type structural ester can be further improved by the synergistic effect of the compound of 1, 3-dioleic acid-2-caprylic triglyceride, 1, 3-dioleic acid-2-capric triglyceride, 1, 3-dioleic acid-2-lauric triglyceride and 1, 3-dioleic acid-2-myristic triglyceride; comparing examples 1 and 8-10, the invention can find that the efficacy of piglet structure fat can be further improved by adopting the compounding of the OMO type structure ester, the coix seed oil and the chili oil and the synergistic effect; compared with the examples 1 and 11-13, the invention can find that the effect of the inulin short-chain fatty acid ester can be effectively improved by adopting the compounding of the inulin propionate, the inulin acetate and the inulin butyrate and the synergistic effect; compared with the embodiments 1 and 14-17, the invention can find that the effect of the polysaccharide complex can be improved by adopting the compounding of the lentinan, the rosa roxburghii tratt polysaccharide, the hericium erinaceus polysaccharide and the alfalfa polysaccharide and the synergistic effect; compared with the embodiment 1 and the comparative examples 1-3, the piglet feed additive disclosed by the invention has the advantages that the specific piglet structural fat, the inulin short-chain fatty acid ester and the polysaccharide compound are compounded and have a synergistic effect, so that the growth and development of piglet intestinal villi are effectively promoted, the ratio of the height of the villi to the depth of a crypt is improved, the digestion and absorption capacity of piglets on the feed is improved, the feed ratio is reduced, the growth of piglets is promoted, and the diarrhea condition is reduced.

The applicant states that the functional compound for promoting piglet growth and the preparation method and application thereof are illustrated by the above examples, but the invention is not limited to the above examples, i.e. the invention is not limited to be implemented by the above examples. It should be understood by those skilled in the art that any modifications to the present invention, equivalent substitutions of the raw materials of the product of the present invention, and the addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.

The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications all fall within the protection scope of the present invention.

It should be noted that the technical features described in the above embodiments can be combined in any suitable manner without contradiction, and various possible combinations of the features are not described in order to avoid unnecessary repetition.

Claims

1. The functional compound for promoting the growth of the piglets is characterized by comprising 30-50 parts of piglet structural fat, 1-10 parts of inulin short-chain fatty acid ester, 1-10 parts of polysaccharide compound, 1-5 parts of glycine iron and 1-5 parts of phytase by weight;

the raw materials for preparing the piglet structure fat comprise 1, 3-dioleic acid-2-medium chain fatty acid triglyceride, coix seed oil and chili oil.

2. The functional compound as claimed in claim 1, wherein the raw materials for preparing piglet structural fat comprise, by weight, 60-80 parts of 1, 3-dioleate-2-medium chain fatty acid triglyceride, 1-5 parts of coix seed oil and 1-10 parts of chili oil;

preferably, the raw materials for preparing the piglet structure fat also comprise a carrier;

3. The functional complex of claim 1 or 2, wherein the 1, 3-dioleic-2-medium chain fatty acid triglycerides comprise 1, 3-dioleic-2-caprylic acid triglyceride, 1, 3-dioleic-2-capric acid triglyceride, 1, 3-dioleic-2-lauric acid triglyceride, and 1, 3-dioleic-2-myristic acid triglyceride;

preferably, the mass ratio of the 1, 3-dioleic-2-caprylic acid triglyceride, the 1, 3-dioleic-2-capric acid triglyceride, the 1, 3-dioleic-2-lauric acid triglyceride and the 1, 3-dioleic-2-myristic acid triglyceride is (1-3): (1-5): (1-2): (3-5).

4. The functional complex of claim 2 or 3, wherein the carrier comprises any one of casein, lactose or soy protein isolate or a combination of at least two thereof;

preferably, the carrier is a combination of casein, lactose, soy protein isolate;

preferably, the mass ratio of the casein to the lactose to the soy protein isolate is (1-3) to (1-2) to 1.

5. The functional complex according to any one of claims 2 to 4, wherein the total mass of the 1, 3-dioleoyl-2-medium chain fatty acid triglyceride, the coix seed oil, the capsicum oil and the carrier is in a mass ratio of (2-3) to (2-3).

6. The functional complex according to any of claims 1-5, wherein the inulin short-chain fatty acid ester comprises inulin acetate, inulin propionate and inulin butyrate;

preferably, the mass ratio of the inulin acetate, the inulin propionate and the inulin butyrate is (9-15): (5-8): (3-5).

7. The functional complex according to any of claims 1-6, wherein the inulin short-chain fatty acid ester has an average degree of polymerization of not more than 5.

8. The functional complex according to any one of claims 1 to 7, wherein the polysaccharide complex comprises Hericium erinaceus polysaccharide, lentinan, Rosa roxburghii polysaccharide and alfalfa polysaccharide;

preferably, the mass ratio of the hericium erinaceus polysaccharide to the lentinan to the rosa roxburghii polysaccharide to the alfalfa polysaccharide is (3-5): 1-2): 3-7): 2-4.

9. A method for preparing a functional complex according to any of claims 1 to 8, comprising the steps of:

10. Use of a functional complex according to any one of claims 1-8 for the preparation of a piglet feed.