CN115005325A - Preparation method of selenium-enriched yeast culture - Google Patents
Preparation method of selenium-enriched yeast culture Download PDFInfo
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- CN115005325A CN115005325A CN202210386042.8A CN202210386042A CN115005325A CN 115005325 A CN115005325 A CN 115005325A CN 202210386042 A CN202210386042 A CN 202210386042A CN 115005325 A CN115005325 A CN 115005325A
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- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 288
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 172
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 172
- 239000011669 selenium Substances 0.000 title claims abstract description 172
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 238000000855 fermentation Methods 0.000 claims abstract description 68
- 230000004151 fermentation Effects 0.000 claims abstract description 68
- 239000000463 material Substances 0.000 claims abstract description 40
- 230000001007 puffing effect Effects 0.000 claims abstract description 39
- 238000002156 mixing Methods 0.000 claims abstract description 34
- 238000001035 drying Methods 0.000 claims description 37
- 239000007788 liquid Substances 0.000 claims description 34
- 239000000203 mixture Substances 0.000 claims description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- 238000000034 method Methods 0.000 claims description 16
- 230000014759 maintenance of location Effects 0.000 claims description 8
- 235000012437 puffed product Nutrition 0.000 claims description 2
- 239000012141 concentrate Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 17
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 36
- 239000001963 growth medium Substances 0.000 description 19
- 229910052757 nitrogen Inorganic materials 0.000 description 18
- 229910052799 carbon Inorganic materials 0.000 description 15
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 14
- 241001465754 Metazoa Species 0.000 description 7
- 239000003205 fragrance Substances 0.000 description 7
- 229910017053 inorganic salt Inorganic materials 0.000 description 7
- 150000003342 selenium Chemical class 0.000 description 7
- 238000010563 solid-state fermentation Methods 0.000 description 7
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 6
- 241000209094 Oryza Species 0.000 description 6
- 235000007164 Oryza sativa Nutrition 0.000 description 6
- 244000046052 Phaseolus vulgaris Species 0.000 description 6
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 6
- 235000009566 rice Nutrition 0.000 description 6
- 235000012054 meals Nutrition 0.000 description 5
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 4
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 4
- 235000011130 ammonium sulphate Nutrition 0.000 description 4
- 125000001477 organic nitrogen group Chemical group 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 235000019270 ammonium chloride Nutrition 0.000 description 3
- 239000004202 carbamide Substances 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 239000000839 emulsion Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 235000013922 glutamic acid Nutrition 0.000 description 3
- 239000004220 glutamic acid Substances 0.000 description 3
- 235000013379 molasses Nutrition 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- 210000005253 yeast cell Anatomy 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 239000005696 Diammonium phosphate Substances 0.000 description 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 2
- 235000019764 Soybean Meal Nutrition 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 description 2
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 2
- 229910000388 diammonium phosphate Inorganic materials 0.000 description 2
- 235000019838 diammonium phosphate Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 235000019837 monoammonium phosphate Nutrition 0.000 description 2
- 239000006012 monoammonium phosphate Substances 0.000 description 2
- 239000004455 soybean meal Substances 0.000 description 2
- 238000001694 spray drying Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 125000000185 sucrose group Chemical group 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000002036 drum drying Methods 0.000 description 1
- 230000036449 good health Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- -1 molasses Chemical class 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
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- Animal Husbandry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Botany (AREA)
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Abstract
The invention discloses a preparation method of a selenium-enriched yeast culture, which comprises the following steps: 1. puffing the yeast culture to obtain a puffed yeast culture; 2. concentrating selenium-enriched yeast fermentation liquor to obtain selenium-enriched yeast concentrated liquor; 3. and (3) mixing the puffed material of the yeast culture prepared in the step (1) with the selenium-enriched yeast concentrated solution prepared in the step (2). The preparation method of the selenium-enriched yeast culture can reduce the production cost of the selenium-enriched yeast, simultaneously reduces the difficulty of adding the mixed selenium-enriched yeast in downstream feed enterprises, ensures that materials are easily and uniformly mixed, can shorten the mixing processing time, improves the production processing efficiency of the feed enterprises, and is more convenient for adding the selenium-enriched yeast in the downstream feed enterprises.
Description
Technical Field
The invention relates to the field of biological feed, and in particular relates to a preparation method of a selenium-enriched yeast culture.
Background
Selenium plays an important physiological role in the body. By combining the recent research progress, the compound preparation can effectively eliminate oxygen free radicals in the body, also can resist inflammation, keeps animals in a good health state, improves the growth performance of the animals, and becomes a more important trace element under the culture trends of antibiotic limitation, resistance reduction and no resistance. In recent years, the selenium-enriched yeast industry in China is rapidly developed and has a high share in the world market. The current global production direction of selenium-enriched yeast seeks to enrich higher selenium content in the product, and the content of some products has been increased to 3000 ppm. In addition, in terms of the drying process in the process, the selenium-enriched yeast emulsion before drying, namely the selenium-enriched yeast concentrated solution, is in an emulsion state without viscosity, so the concentrated yeast solution is directly dried by adopting a spray drying or scraper roller drying mode. The drying efficiency of the two drying modes is low, so that the production cost of the selenium-enriched yeast is always in a high level, and difficulty is brought to the popularization and the use of the selenium-enriched yeast.
Efforts to increase yeast conversion to inorganic selenium are certainly good, but not necessarily the best option if products are offered to the market at levels of 3000ppm or more. As selenium is a trace element for animals, the addition amount of selenium in animal feed is very small, the recommended amount in most national feeding standards is only 0.3g of selenium per ton of complete pig ration, and the amount of selenium is only 100g of yeast converted into 3000 ppm. In addition, because the selenium is toxic in excess, the highest addition amount is limited in most countries, and the highest addition amount of the complete material specified in China is 0.5ppm, namely 0.5g of the complete material per ton, and only 166.7g of yeast selenium converted into 3000 ppm. The small addition amount brings great difficulty to the uniform mixing of the addition in the downstream feed production, and the production cost is increased because long mixing processing time is needed for ensuring the uniform mixing of materials. Moreover, once the mixture is not uniform, the selenium supplementing effect of animals can be affected; if the feed sample is determined by spot check, the selenium content can exceed the standard, which brings trouble to production and operation and causes economic loss. In view of the above problems, it is very necessary to develop a novel selenium-enriched yeast product which can reduce the production cost of selenium-enriched yeast and reduce the difficulty of adding and mixing the selenium-enriched yeast in downstream feed enterprises.
Disclosure of Invention
The invention aims to provide a preparation method of a selenium-enriched yeast culture, which aims to solve the problems in the background art, and the preparation method of the selenium-enriched yeast culture can reduce the production cost of the selenium-enriched yeast, simultaneously reduces the difficulty of adding and mixing the selenium-enriched yeast for downstream feed enterprises, ensures that materials are easily mixed uniformly, can shorten the mixing processing time, improves the production and processing efficiency of the feed enterprises, and is more convenient for the downstream feed enterprises to add the selenium-enriched yeast.
In order to achieve the purpose, the invention provides the following technical scheme:
a preparation method of a selenium-enriched yeast culture comprises the following steps:
1. puffing the yeast culture to obtain a puffed yeast culture;
2. concentrating the selenium-enriched yeast fermentation liquor to obtain a selenium-enriched yeast concentrated solution;
3. and (3) mixing the puffed material of the yeast culture prepared in the step (1) with the selenium-enriched yeast concentrated solution prepared in the step (2).
Preferably, in the step 1, the yeast culture is prepared by adding water into a culture medium, mixing, inoculating yeast, and performing solid state fermentation, wherein the prepared yeast culture is a fermentation product with yeast fermentation fragrance, consistent color, no foreign odor and no mildew, and the culture medium is one or more of meals, grains, bean dregs, fruit residues, bran and rice bran.
Preferably, in the step 2, the selenium-enriched yeast fermentation broth is prepared by liquid fermentation, wherein the selenium content of the selenium-enriched yeast fermentation broth is 120-180ppm, and the yeast content of the selenium-enriched yeast fermentation broth is 3-7%.
Preferably, the selenium-enriched yeast fermentation liquor is prepared by liquid fermentation of a carbon source, a nitrogen source, inorganic salt, selenium salt and yeast; preferably, the carbon source is a carbohydrate (e.g., molasses, glucose, sucrose, etc.); the nitrogen source is organic nitrogen source (such as glutamic acid, urea, etc.) or inorganic nitrogen source (such as ammonium sulfate, ammonium chloride, monoammonium phosphate, diammonium phosphate).
Preferably, in step 1, the yeast culture is dried before being expanded.
Preferably, the yeast culture is dried before being puffed, and the moisture content of the yeast culture after drying is as follows: the water content of the yeast culture is more than or equal to 8 percent and less than 13 percent.
Preferably, the yeast culture is dried before being puffed, and the moisture content of the yeast culture after drying is as follows: the water content of the yeast culture is more than or equal to 13 percent and less than or equal to 30 percent.
Preferably, in the step 1, the puffing temperature of the puffing treatment of the yeast culture is 90-130 ℃, and the retention time of the materials in the puffing cavity is 10-30 seconds.
Preferably, in the step 2, the selenium-enriched yeast concentrated solution obtained contains 800ppm of selenium with the content of 550-800ppm and 75-85% of water.
Preferably, in the step 3, the puffed material of the yeast culture is mixed with the selenium-enriched yeast concentrated solution according to the weight part ratio of 1 (3-8).
Preferably, the mixture obtained by mixing the puffed yeast culture in the step 3 with the selenium-enriched yeast concentrated solution is dried.
Preferably, the mixture obtained in step 3 is dried by any one of a fluidized bed dryer, a rotary drum dryer, a belt dryer, a combination of a rotary drum dryer and a fluidized bed dryer, and a combination of a belt dryer and a fluidized bed dryer.
The selenium content in the selenium-enriched yeast culture prepared by the method is about 500-1500 ppm.
According to the flexible configuration requirement of production, the invention can integrate various low-cost drying modes and configure the drying modes into a combination of multiple drying modes.
The invention has the beneficial effects that:
in the prior art, selenium-enriched yeast and yeast culture are respectively used as two products in the feed and breeding industries. The invention combines the selenium-enriched yeast and the yeast culture into a whole by the nutrition concept and the process innovation. The product prepared by the invention has more comprehensive nutritional effect, improves the drying mode by changing the physical state of the dried material, reduces the production cost and is more convenient for downstream enterprises to use better.
Further, on the basis of years of research and practice, the method mixes the common yeast culture with the selenium-enriched yeast emulsion (namely the selenium-enriched yeast concentrated solution in the invention) obtained after centrifugation in the production of the selenium-enriched yeast, and utilizes drying technologies with high capacity and high efficiency, such as a fluidized bed dryer, a rotary cylinder dryer, a belt dryer and the like to replace the drying technologies with low capacity and low efficiency of spray drying or scraper drum drying of the conventional selenium-enriched yeast manufacturers, so that the production cost of the selenium-enriched yeast is greatly reduced, and the low-carbon development requirement is better met.
In addition, the invention also creatively utilizes the puffing technology to carry out puffing treatment on the common yeast culture, so that the puffed yeast culture is easier to digest and absorb and has better water absorption. The puffing technique is to expand the puffed material by suddenly reducing the pressure under heating and pressurizing conditions. The tissue structure of the puffed material is changed, and the originally wrapped nutrient substances and active ingredients are fully released, so that the puffed material is convenient for digestion and absorption of animals. The expanded material becomes porous and fluffy, and the water absorption of the expanded material becomes stronger.
The nutritional value of yeast cultures is not in live bacteria, mainly metabolites, degraded media and yeast body nutrients or yeast cell components. The yeast metabolite still exists in the yeast culture after the expansion, and during the expansion treatment of the yeast culture in the step 1, the yeast cells in the yeast culture are ruptured to a certain degree, so that the yeast cell walls are deformed or ruptured, the contents are released more easily, and the contents are used by animals more easily. Moreover, in the step 3 of the invention, the puffed material of the yeast culture needs to be mixed with the selenium-enriched yeast concentrated solution, the selenium-enriched yeast concentrated solution in the invention is the selenium-enriched yeast concentrated solution with high yeast content obtained by centrifuging after yeast liquid fermentation, the number of yeast bacteria of the mixed mixture is improved by multiple times compared with that of the common yeast culture, and the added selenium-enriched yeast concentrated solution contains active yeast which can continuously carry out biochemical reaction with free components obtained by physical change after puffing in the adding process to generate new active nutrient substances.
The selenium-enriched yeast culture product prepared by the preparation method of the selenium-enriched yeast culture has higher palatability and is easier to absorb, digest and utilize. Compared with the commercial selenium-enriched yeast product with the same selenium content, the production cost of the selenium-enriched yeast culture prepared by the invention is reduced by 12-31% through the system improvement of the selenium-enriched yeast preparation process, the selenium content in the selenium-enriched yeast culture prepared by the invention can be controlled to be 500 plus materials 1500ppm, the preparation method disclosed by the invention not only achieves the purpose of reducing the production cost, but also achieves the premixing treatment of selenium before the use of feed enterprises, reduces the mixing difficulty when the feed enterprises use, shortens the mixing duration when the feed enterprises use, and achieves the effect of killing two birds with one stone.
The process of using the selenium-enriched yeast culture of the invention is very convenient for downstream feed enterprises, compared with the commercial selenium-enriched yeast with high selenium content, the selenium-enriched yeast culture prepared by the invention is easier to be uniformly mixed when being used for preparing feed mixtures, the mixing time can be shortened by 16-33%, the production and processing efficiency of the feed enterprises is improved, and the feed processing cost is further reduced from another aspect.
Detailed Description
For better understanding of the present invention, the following examples are given for further illustration of the present invention, but the present invention is not limited to the following examples.
Example 1
A preparation method of a selenium-enriched yeast culture comprises the following steps:
1. puffing the yeast culture to obtain puffed yeast culture;
2. concentrating the selenium-enriched yeast fermentation liquor to obtain a selenium-enriched yeast concentrated solution;
3. and (3) mixing the puffed material of the yeast culture prepared in the step (1) with the selenium-enriched yeast concentrated solution prepared in the step (2).
In the step 1, the yeast culture is prepared by adding water into a culture medium, mixing, inoculating yeast, and performing solid state fermentation, wherein the prepared yeast culture is a fermentation product with yeast fermentation fragrance, consistent color, no foreign odor and no mildew, and the culture medium is bean dregs.
In the step 2, the selenium-enriched yeast fermentation liquid is prepared by liquid fermentation, wherein the selenium content of the selenium-enriched yeast fermentation liquid is 120ppm, and the yeast content of the selenium-enriched yeast fermentation liquid is 6.9%.
The selenium-enriched yeast fermentation liquor is prepared by liquid fermentation of a carbon source, a nitrogen source, inorganic salt, selenium salt and yeast; preferably, the carbon source is molasses; the nitrogen source is an organic nitrogen source (specifically glutamic acid).
In step 1, the yeast culture is dried before being expanded.
The yeast culture is dried before being puffed, and the moisture content of the yeast culture after drying is 8%.
In the step 1, the puffing temperature of the puffing treatment of the yeast culture is 90 ℃, and the retention time of the materials in the puffing cavity is 15 seconds.
In the step 2, the selenium content in the selenium-enriched yeast concentrated solution is 550ppm, and the water content is 81%.
In the step 3, the puffed material of the yeast culture and the selenium-enriched yeast concentrated solution are mixed according to the weight ratio of 1: 3.
And (4) drying the mixture obtained by mixing the puffed yeast culture obtained in the step (3) with the selenium-enriched yeast concentrated solution.
And (4) drying the mixture obtained in the step (3) by using a fluidized bed dryer.
Example 2
A preparation method of a selenium-enriched yeast culture comprises the following steps:
1. puffing the yeast culture to obtain a puffed yeast culture;
2. concentrating the selenium-enriched yeast fermentation liquor to obtain a selenium-enriched yeast concentrated solution;
3. and (3) mixing the puffed material of the yeast culture prepared in the step (1) with the selenium-enriched yeast concentrated solution prepared in the step (2).
In the step 1, the yeast culture is prepared by adding water into a culture medium, mixing the culture medium with the water, inoculating yeast, and performing solid state fermentation, wherein the prepared yeast culture is a fermentation product with yeast fermentation fragrance, consistent color, no foreign odor and no mildew, and the culture medium is prepared from bran and rice bran according to the weight part ratio of (2): 5, and (c) preparing a mixture.
In the step 2, the selenium-enriched yeast fermentation liquid is prepared by liquid fermentation, wherein the selenium content of the selenium-enriched yeast fermentation liquid is 155ppm, and the yeast content of the selenium-enriched yeast fermentation liquid is 4.3%.
The selenium-enriched yeast fermentation liquor is prepared by liquid fermentation of a carbon source, a nitrogen source, inorganic salt, selenium salt and yeast; preferably, the carbon source is glucose; the nitrogen source is an organic nitrogen source (specifically urea).
In step 1, the yeast culture is dried before being expanded.
The yeast culture is dried before being puffed, and the moisture content of the dried yeast culture is 13%.
In the step 1, the puffing temperature of the puffing treatment of the yeast culture is 130 ℃, and the retention time of the materials in the puffing cavity is 30 seconds.
In the step 2, the selenium content in the selenium-enriched yeast concentrated solution is 583ppm, and the water content is 85%.
In the step 3, the puffed material of the yeast culture and the selenium-enriched yeast concentrated solution are mixed according to the weight ratio of 1: 4.
And (4) drying the mixture obtained by mixing the puffed yeast culture obtained in the step (3) with the selenium-enriched yeast concentrated solution.
And (4) drying the mixture obtained in the step (3) by using a rotary cylinder dryer.
Example 3
A preparation method of a selenium-enriched yeast culture comprises the following steps:
1. puffing the yeast culture to obtain a puffed yeast culture;
2. concentrating the selenium-enriched yeast fermentation liquor to obtain a selenium-enriched yeast concentrated solution;
3. and (3) mixing the puffed material of the yeast culture prepared in the step (1) with the selenium-enriched yeast concentrated solution prepared in the step (2).
In the step 1, the yeast culture is prepared by adding water into a culture medium, mixing the culture medium with the water, inoculating yeast, and performing solid state fermentation, wherein the prepared yeast culture is a fermented product with yeast fermentation fragrance, consistent color and luster, no foreign odor and no mildew, and the culture medium is a meal (soybean meal), grains (corn) and bean dregs in a weight ratio of 6: 2: 2, and (c) 2.
In the step 2, the selenium-enriched yeast fermentation liquid is prepared by liquid fermentation, wherein the selenium content of the selenium-enriched yeast fermentation liquid is 168ppm, and the yeast content of the selenium-enriched yeast fermentation liquid is 3%.
The selenium-enriched yeast fermentation liquor is prepared by liquid fermentation of a carbon source, a nitrogen source, inorganic salt, selenium salt and yeast; preferably, the carbon source is sucrose; the nitrogen source is an organic nitrogen source (specifically, a mixture of glutamic acid and urea in a weight ratio of 7: 3).
In step 1, the yeast culture is dried before being expanded.
The yeast culture is dried before being puffed, and the moisture content of the yeast culture after drying is 11%.
In the step 1, the puffing temperature of the puffing treatment of the yeast culture is 116 ℃, and the retention time of the materials in the puffing cavity is 21 seconds.
In the step 2, the selenium content in the selenium-enriched yeast concentrated solution is 778ppm, and the water content is 83%.
In the step 3, the puffed material of the yeast culture and the selenium-enriched yeast concentrated solution are mixed according to the weight ratio of 1: 8.
And (4) drying the mixture obtained by mixing the puffed yeast culture obtained in the step (3) with the selenium-enriched yeast concentrated solution.
And (4) drying the mixture obtained in the step (3) by using a belt dryer.
Example 4
A preparation method of a selenium-enriched yeast culture comprises the following steps:
1. puffing the yeast culture to obtain puffed yeast culture;
2. concentrating selenium-enriched yeast fermentation liquor to obtain selenium-enriched yeast concentrated liquor;
3. and (3) mixing the puffed product of the yeast culture prepared in the step (1) with the selenium-enriched yeast concentrated solution prepared in the step (2).
In the step 1, the yeast culture is prepared by adding water into a culture medium, mixing the culture medium with the water, inoculating yeast, and performing solid state fermentation, wherein the prepared yeast culture is a fermentation product with yeast fermentation fragrance, consistent color, no foreign odor and no mildew, and the culture medium is meal (rice bran meal), grains (corn), bean dregs, pomace, bran and rice bran in a weight ratio of 6: 2:1: 1: 2:1 of the resulting mixture.
In the step 2, the selenium-enriched yeast fermentation liquid is prepared by liquid fermentation, wherein the selenium content of the selenium-enriched yeast fermentation liquid is 173ppm, and the yeast content of the selenium-enriched yeast fermentation liquid is 5.6%.
The selenium-enriched yeast fermentation liquor is prepared by liquid fermentation of a carbon source, a nitrogen source, inorganic salt, selenium salt and yeast; preferably, the carbon source is glucose; the nitrogen source is an inorganic nitrogen source (specifically ammonium sulfate).
In step 1, the yeast culture is dried before being expanded.
The yeast culture is dried before being puffed, and the moisture content of the yeast culture after drying is 30%.
In the step 1, the puffing temperature of the puffing treatment of the yeast culture is 110 ℃, and the retention time of the materials in the puffing cavity is 25 seconds.
In the step 2, the selenium content in the selenium-enriched yeast concentrated solution is 780ppm, and the water content is 75%.
In the step 3, the puffed material of the yeast culture and the selenium-enriched yeast concentrated solution are mixed according to the weight ratio of 1: 7.
And (4) drying the mixture obtained by mixing the puffed yeast culture obtained in the step (3) with the selenium-enriched yeast concentrated solution.
And (4) drying the mixture obtained in the step (3) by adopting a combination of a rotary cylinder dryer and a fluidized bed dryer, namely, firstly performing first-step drying treatment by adopting the rotary cylinder dryer, and then performing second-step drying treatment on the material treated by the rotary cylinder dryer by using the fluidized bed dryer.
Example 5
A preparation method of a selenium-enriched yeast culture comprises the following steps:
1. puffing the yeast culture to obtain a puffed yeast culture;
2. concentrating selenium-enriched yeast fermentation liquor to obtain selenium-enriched yeast concentrated liquor;
3. and (3) mixing the puffed material of the yeast culture prepared in the step (1) with the selenium-enriched yeast concentrated solution prepared in the step (2).
In the step 1, the yeast culture is not dried before being subjected to expansion treatment, the moisture content of the yeast culture is 35%, and the yeast culture directly enters an expansion machine for expansion treatment to obtain the expanded yeast culture.
In the step 1, the yeast culture is prepared by adding water into a culture medium, mixing the culture medium with the water, inoculating yeast, and performing solid state fermentation, wherein the prepared yeast culture is a fermentation product with yeast fermentation fragrance, consistent color, no foreign odor and no mildew, and the culture medium is bean dregs, fruit residues, bran and rice bran in a weight ratio of 4: 3: 1: 2, and (c) 2.
In the step 2, the selenium-enriched yeast fermentation liquid is prepared by liquid fermentation, wherein the selenium content of the selenium-enriched yeast fermentation liquid is 157ppm, and the yeast content of the selenium-enriched yeast fermentation liquid is 4.25%.
The selenium-enriched yeast fermentation liquor is prepared by liquid fermentation of a carbon source, a nitrogen source, inorganic salt, selenium salt and yeast; preferably, the carbon source is molasses; the nitrogen source is an inorganic nitrogen source (specifically, a mixture of ammonium sulfate and ammonium chloride in a weight ratio of 5: 1).
In the step 1, the puffing temperature of the puffing treatment of the yeast culture is 105 ℃, and the retention time of the materials in the puffing cavity is 25 seconds.
In the step 2, the selenium content in the selenium-enriched yeast concentrated solution is 782ppm, and the water content is 84%.
In the step 3, the puffed material of the yeast culture and the selenium-enriched yeast concentrated solution are mixed according to the weight ratio of 1: 6.
And (4) drying the mixture obtained by mixing the puffed yeast culture obtained in the step (3) with the selenium-enriched yeast concentrated solution.
And (3) drying the mixture obtained in the step (3) by adopting a combination of a rotary cylinder dryer and a fluidized bed dryer, namely firstly drying the mixture by adopting the rotary cylinder dryer for the first step, and then drying the material treated by the rotary cylinder dryer for the second step by using the fluidized bed dryer.
Example 6
A preparation method of a selenium-enriched yeast culture comprises the following steps:
1. puffing the yeast culture to obtain a puffed yeast culture;
2. concentrating selenium-enriched yeast fermentation liquor to obtain selenium-enriched yeast concentrated liquor;
3. and (3) mixing the puffed material of the yeast culture prepared in the step (1) with the selenium-enriched yeast concentrated solution prepared in the step (2).
In the step 1, the yeast culture is prepared by adding water into a culture medium, mixing the culture medium with the water, inoculating yeast, and performing solid state fermentation, wherein the prepared yeast culture is a fermented product with yeast fermentation fragrance, consistent color, no foreign odor and no mildew, and the culture medium is meal (soybean meal), bean dregs, fruit residues, bran and rice bran in a weight ratio of 5:2: 2:1: 2, and (c) preparing a mixture.
In the step 2, the selenium-enriched yeast fermentation liquid is prepared by liquid fermentation, wherein the selenium content of the selenium-enriched yeast fermentation liquid is 180ppm, and the yeast content of the selenium-enriched yeast fermentation liquid is 7%.
Preferably, the selenium-enriched yeast fermentation liquor is prepared by liquid fermentation of a carbon source, a nitrogen source, inorganic salt, selenium salt and yeast; preferably, the carbon source is sucrose; the nitrogen source is an inorganic nitrogen source (specifically a mixture of ammonium sulfate, ammonium chloride, monoammonium phosphate and diammonium phosphate according to a weight ratio of 5:2:1: 1).
In step 1, the yeast culture is dried before being expanded.
The yeast culture is dried before being puffed, and the moisture content of the yeast culture after drying is 23%.
In the step 1, the puffing temperature of the puffing treatment of the yeast culture is 126 ℃, and the retention time of the materials in the puffing cavity is 10 seconds.
In the step 2, the selenium content in the selenium-enriched yeast concentrated solution is 800ppm, and the water content is 77%.
In the step 3, the puffed material of the yeast culture and the selenium-enriched yeast concentrated solution are mixed according to the weight ratio of 1: 5.
And (4) drying the mixture obtained by mixing the puffed yeast culture obtained in the step (3) with the selenium-enriched yeast concentrated solution.
And (4) drying the mixture obtained in the step (3) by adopting a combination of a belt dryer and a fluidized bed dryer, namely, firstly performing first-step drying treatment by adopting the belt dryer, and then performing second-step drying treatment on the material treated by the belt dryer by using the fluidized bed dryer.
Claims (10)
1. The preparation method of the selenium-enriched yeast culture is characterized by comprising the following steps of:
1. puffing the yeast culture to obtain puffed yeast culture;
2. concentrating the selenium-enriched yeast fermentation liquor to obtain a selenium-enriched yeast concentrated solution;
3. and (3) mixing the puffed material of the yeast culture prepared in the step (1) with the selenium-enriched yeast concentrated solution prepared in the step (2).
2. The method as claimed in claim 1, wherein in step 2, the selenium-enriched yeast fermentation broth is obtained by liquid fermentation, wherein the selenium content of the selenium-enriched yeast fermentation broth is 120-180ppm, and the yeast content of the selenium-enriched yeast fermentation broth is 3-7%.
3. The method for preparing the selenium-enriched yeast culture as claimed in claim 1, wherein in step 1, the yeast culture is dried before being puffed.
4. The method for preparing the selenium-enriched yeast culture as claimed in claim 3, wherein the yeast culture is dried before puffing, and the moisture content of the yeast culture after drying is as follows: the water content of the yeast culture is more than or equal to 8 percent and less than 13 percent.
5. The method for preparing the selenium-enriched yeast culture as claimed in claim 3, wherein the yeast culture is dried before puffing, and the moisture content of the yeast culture after drying is as follows: the water content of the yeast culture is more than or equal to 13 percent and less than or equal to 30 percent.
6. The method for preparing the selenium-enriched yeast culture as claimed in claim 1, wherein in the step 1, the puffing temperature of the puffing treatment of the yeast culture is 90-130 ℃, and the retention time of the material in the puffing cavity is 10-30 seconds.
7. The method as claimed in claim 1, wherein the selenium-enriched yeast concentrate obtained in step 2 has a selenium content of 550-800ppm and a water content of 75-85%.
8. The method for preparing the selenium-enriched yeast culture according to claim 1, wherein in the step 3, the puffed material of the yeast culture is mixed with the selenium-enriched yeast concentrated solution according to the weight part of 1 (3-8).
9. The method for preparing the selenium-enriched yeast culture as claimed in claim 1, wherein the mixture obtained by mixing the puffed product of the yeast culture in step 3 with the selenium-enriched yeast concentrated solution is dried.
10. The method for preparing a selenium-enriched yeast culture as claimed in claim 1, wherein the mixture obtained in step 3 is dried by any one of a fluidized bed dryer, a rotary drum dryer, a belt dryer, a combination of a rotary drum dryer and a fluidized bed dryer, and a combination of a belt dryer and a fluidized bed dryer.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101709274A (en) * | 2009-11-27 | 2010-05-19 | 华南农业大学 | Selenium-enriched yeast and preparation method thereof |
| CN103918875A (en) * | 2014-04-11 | 2014-07-16 | 沈阳市康普利德生物科技有限公司 | Preparation method of cured fermented feed |
| CN105519802A (en) * | 2015-12-03 | 2016-04-27 | 安徽五粮泰生物工程股份有限公司 | Preparation method of food-calling puffing fermented creep feed for piggies |
| CN113383857A (en) * | 2021-07-08 | 2021-09-14 | 四川省畜牧科学研究院 | Method for treating fermented rapeseed processing by-products |
| CN113519708A (en) * | 2020-04-20 | 2021-10-22 | 安琪酵母股份有限公司 | Yeast composition for improving weaning feed intake of piglets and reducing diarrhea rate, and preparation and application thereof |
-
2022
- 2022-04-13 CN CN202210386042.8A patent/CN115005325A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101709274A (en) * | 2009-11-27 | 2010-05-19 | 华南农业大学 | Selenium-enriched yeast and preparation method thereof |
| CN103918875A (en) * | 2014-04-11 | 2014-07-16 | 沈阳市康普利德生物科技有限公司 | Preparation method of cured fermented feed |
| CN105519802A (en) * | 2015-12-03 | 2016-04-27 | 安徽五粮泰生物工程股份有限公司 | Preparation method of food-calling puffing fermented creep feed for piggies |
| CN113519708A (en) * | 2020-04-20 | 2021-10-22 | 安琪酵母股份有限公司 | Yeast composition for improving weaning feed intake of piglets and reducing diarrhea rate, and preparation and application thereof |
| CN113383857A (en) * | 2021-07-08 | 2021-09-14 | 四川省畜牧科学研究院 | Method for treating fermented rapeseed processing by-products |
Non-Patent Citations (1)
| Title |
|---|
| 赵晓丹: "食物抗营养因子", vol. 1, 中国农业大学出版社, pages: 119 * |
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