CN115003687B - Hmo生产 - Google Patents

Hmo生产 Download PDF

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Publication number
CN115003687B
CN115003687B CN202180009967.4A CN202180009967A CN115003687B CN 115003687 B CN115003687 B CN 115003687B CN 202180009967 A CN202180009967 A CN 202180009967A CN 115003687 B CN115003687 B CN 115003687B
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CN
China
Prior art keywords
nucleic acid
hmos
hmo
gene
genetically modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202180009967.4A
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English (en)
Chinese (zh)
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CN115003687A (zh
Inventor
M·彼得森
K·B·坎普曼
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Glycom AS
Original Assignee
Glycom AS
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202180009967.4A 2020-01-23 2021-01-22 Hmo生产 Active CN115003687B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA202000086 2020-01-23
DKPA202000086 2020-01-23
PCT/EP2021/051472 WO2021148614A1 (en) 2020-01-23 2021-01-22 Hmo production

Publications (2)

Publication Number Publication Date
CN115003687A CN115003687A (zh) 2022-09-02
CN115003687B true CN115003687B (zh) 2024-12-06

Family

ID=74556857

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180009967.4A Active CN115003687B (zh) 2020-01-23 2021-01-22 Hmo生产

Country Status (7)

Country Link
US (1) US12428657B2 (https=)
EP (1) EP4093747A1 (https=)
JP (1) JP2023511522A (https=)
CN (1) CN115003687B (https=)
AU (1) AU2021210607A1 (https=)
BR (1) BR112022014367A2 (https=)
WO (1) WO2021148614A1 (https=)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK180952B1 (en) * 2020-12-22 2022-08-10 Glycom As A dfl-producing strain
CN116745430A (zh) * 2021-01-20 2023-09-12 因比奥斯公司 在宿主细胞中产生包含ln3作为核心结构的寡糖
BR112023014557A2 (pt) 2021-01-22 2023-10-17 Glycom As Nova proteína (fred) da superfamília de facilitadores principais (mfs) na produção de hmos sialiado
DK181991B1 (en) 2021-11-11 2025-05-15 Dsm Ip Assets Bv Combined fermentation process for producing one or more human milk oligosaccharide(s) (hmo(s)) in escherichia coli
US20250290078A1 (en) 2022-04-29 2025-09-18 Dsm Ip Assets B.V. Hmo producing microorganism with increased robustness towards glucose gradients
DK182075B1 (en) * 2022-06-13 2025-07-02 Dsm Ip Assets Bv Genetically engineered cells comprising sigma factor modifications for production of heterologous products and methods for use of same
DK181765B1 (en) * 2022-07-15 2024-12-04 Dsm Ip Assets Bv Cells expressing new fucosyltransferases for in vivo synthesis of lnfp-iii, and methods and uses of same
EP4577061A1 (en) 2022-08-25 2025-07-02 DSM IP Assets B.V. Hybrid method for producing complex hmos
WO2024110667A1 (en) 2022-11-25 2024-05-30 Dsm Ip Assets B.V. Two-strain system for producing oligosaccharides
CN117867054B (zh) * 2023-01-09 2024-09-27 山东恒鲁生物科技有限公司 合成hmo的方法
WO2024168219A2 (en) * 2023-02-09 2024-08-15 Amyris, Inc. Compositions and methods for improved production of human milk oligosaccharides
CN120677242A (zh) 2023-02-24 2025-09-19 帝斯曼知识产权资产管理有限公司 用于体内合成人乳寡糖的产物特异性转运蛋白
DK181822B1 (en) * 2023-06-07 2025-01-30 Dsm Ip Assets Bv Genetically engineered strains with reduced byproduct formation and methods and uses of same
DK182227B1 (en) 2023-10-17 2025-12-18 Dsm Ip Assets Bv Genetically engineered cells comprising new fucosyltransferases for in vivo synthesis of complex fucosylated human milk oligosaccharides mixtures comprising lnfp-vi, methods using the same, and uses of new fucosyltransferases thereof.
DK202430472A1 (en) 2024-08-19 2026-02-24 Dsm Ip Assets Bv Alpha-1,2 fucosyltransferase mutants with increased alpha 1,3-fucosyltransferase activity

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015150328A1 (en) * 2014-03-31 2015-10-08 Jennewein Biotechnologie Gmbh Total fermentation of oligosaccharides
CN109790559A (zh) * 2016-10-29 2019-05-21 詹尼温生物技术有限责任公司 改良的用于产生岩藻糖基化寡糖的方法
CN112119164A (zh) * 2018-05-16 2020-12-22 詹尼温生物技术有限责任公司 糖苷酶在低聚糖生产中的用途

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2440661T3 (en) * 2009-06-08 2018-03-12 Jennewein Biotechnologie Gmbh HMO synthesis
EP2675899A4 (en) 2011-02-16 2014-09-10 Glycosyn LLC BIOSYNTHESIS OF HUMAN MILK OLIGOSACCHARIDES IN MANIPULATED BACTERIA
DE202015009782U1 (de) 2014-06-11 2020-02-10 Glycom A/S Trennung von 2'-O-Fucosyllactose aus Fermentationsbrühe
EP3191499A4 (en) 2014-09-09 2018-06-06 Glycosyn LLC Alpha (1,3) fucosyltransferases for use in the production of fucosylated oligosaccharides
US10676770B2 (en) 2014-12-16 2020-06-09 Glycom A/S Separation of 2′-FL from a fermentation broth
EP3141610A1 (en) 2015-09-12 2017-03-15 Jennewein Biotechnologie GmbH Production of human milk oligosaccharides in microbial hosts with engineered import / export
DE202017007249U1 (de) 2016-03-07 2020-04-23 Glycom A/S Abtrennung von Oligosacchariden aus der Fermentationsbrühe
EP4674526A3 (en) 2016-04-19 2026-04-01 Glycom A/S Separation of oligosaccharides from fermentation broth
US11608504B2 (en) 2017-12-21 2023-03-21 Glycom A/S Nucleic acid construct for in vitro and in vivo gene expression
US20190323052A1 (en) * 2018-04-23 2019-10-24 Dupont Nutrition Biosciences Aps Increasing export of 2? fucosyllactose from microbial cells through the expression of a heterologous nucleic acid
US20190323053A1 (en) 2018-04-23 2019-10-24 Dupont Nutrition Biosciences Aps Increasing activity of 2? fucosyllactose transporters endogenous to microbial cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015150328A1 (en) * 2014-03-31 2015-10-08 Jennewein Biotechnologie Gmbh Total fermentation of oligosaccharides
CN109790559A (zh) * 2016-10-29 2019-05-21 詹尼温生物技术有限责任公司 改良的用于产生岩藻糖基化寡糖的方法
CN112119164A (zh) * 2018-05-16 2020-12-22 詹尼温生物技术有限责任公司 糖苷酶在低聚糖生产中的用途

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MFS transporter [Serratia marcescens.Genbank Accession：WP_060448169.2016,全文，尤其是ORIGIN部分. *
MFS transporter [Serratia marcescens].Genbank Accession：WP_060448169.2016,全文，尤其是ORIGIN部分. *

Also Published As

Publication number Publication date
JP2023511522A (ja) 2023-03-20
CN115003687A (zh) 2022-09-02
WO2021148614A1 (en) 2021-07-29
AU2021210607A1 (en) 2022-07-14
US12428657B2 (en) 2025-09-30
US20230227876A1 (en) 2023-07-20
BR112022014367A2 (pt) 2022-09-13
EP4093747A1 (en) 2022-11-30

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