CN114982616B - Efficient nitrogen absorption peanut variety screening method and culture observation device - Google Patents
Efficient nitrogen absorption peanut variety screening method and culture observation device Download PDFInfo
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- CN114982616B CN114982616B CN202210702156.9A CN202210702156A CN114982616B CN 114982616 B CN114982616 B CN 114982616B CN 202210702156 A CN202210702156 A CN 202210702156A CN 114982616 B CN114982616 B CN 114982616B
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims abstract description 94
- 235000020232 peanut Nutrition 0.000 title claims abstract description 49
- 229910052757 nitrogen Inorganic materials 0.000 title claims abstract description 47
- 235000017060 Arachis glabrata Nutrition 0.000 title claims abstract description 46
- 235000010777 Arachis hypogaea Nutrition 0.000 title claims abstract description 46
- 235000018262 Arachis monticola Nutrition 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 title claims abstract description 29
- 238000012216 screening Methods 0.000 title claims abstract description 24
- 238000010521 absorption reaction Methods 0.000 title claims abstract description 22
- 241001553178 Arachis glabrata Species 0.000 title abstract description 39
- 230000010496 root system development Effects 0.000 claims abstract description 23
- 239000001963 growth medium Substances 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 230000001502 supplementing effect Effects 0.000 claims description 39
- 238000005286 illumination Methods 0.000 claims description 15
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 12
- 239000001301 oxygen Substances 0.000 claims description 12
- 229910052760 oxygen Inorganic materials 0.000 claims description 12
- 238000007789 sealing Methods 0.000 claims description 10
- 241000196324 Embryophyta Species 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 230000003203 everyday effect Effects 0.000 claims description 5
- 239000011521 glass Substances 0.000 claims description 4
- 244000105624 Arachis hypogaea Species 0.000 claims 10
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims 4
- 235000017491 Bambusa tulda Nutrition 0.000 claims 4
- 241001330002 Bambuseae Species 0.000 claims 4
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims 4
- 239000011425 bamboo Substances 0.000 claims 4
- 238000012258 culturing Methods 0.000 abstract description 5
- 238000001514 detection method Methods 0.000 abstract 1
- 239000003921 oil Substances 0.000 description 11
- 230000008569 process Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 230000021749 root development Effects 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 238000012136 culture method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000010720 hydraulic oil Substances 0.000 description 2
- 238000006213 oxygenation reaction Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 206010021143 Hypoxia Diseases 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F21—LIGHTING
- F21V—FUNCTIONAL FEATURES OR DETAILS OF LIGHTING DEVICES OR SYSTEMS THEREOF; STRUCTURAL COMBINATIONS OF LIGHTING DEVICES WITH OTHER ARTICLES, NOT OTHERWISE PROVIDED FOR
- F21V14/00—Controlling the distribution of the light emitted by adjustment of elements
- F21V14/02—Controlling the distribution of the light emitted by adjustment of elements by movement of light sources
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F21—LIGHTING
- F21V—FUNCTIONAL FEATURES OR DETAILS OF LIGHTING DEVICES OR SYSTEMS THEREOF; STRUCTURAL COMBINATIONS OF LIGHTING DEVICES WITH OTHER ARTICLES, NOT OTHERWISE PROVIDED FOR
- F21V21/00—Supporting, suspending, or attaching arrangements for lighting devices; Hand grips
- F21V21/34—Supporting elements displaceable along a guiding element
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F21—LIGHTING
- F21V—FUNCTIONAL FEATURES OR DETAILS OF LIGHTING DEVICES OR SYSTEMS THEREOF; STRUCTURAL COMBINATIONS OF LIGHTING DEVICES WITH OTHER ARTICLES, NOT OTHERWISE PROVIDED FOR
- F21V33/00—Structural combinations of lighting devices with other articles, not otherwise provided for
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Engineering & Computer Science (AREA)
- Biodiversity & Conservation Biology (AREA)
- Botany (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Cultivation Of Plants (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention belongs to the technical field of crop identification and screening, and discloses a high-efficiency nitrogen absorption peanut variety screening method and a culture observation device thereof. According to the method, the root system development condition is detected by water culture for 12-15 days or culture for 20-25 days in a culture medium under the specific total nitrogen content, the root system development coefficient S is calculated according to the total length of the root system under different nitrogen culture, and then the nitrogen absorption capacity of peanut varieties is judged. The invention also discloses a device for conveniently culturing and observing the peanuts. The invention realizes the rapid batch detection of peanut varieties and ensures the accuracy of identification.
Description
Technical Field
The invention relates to the technical field of crop identification and screening, in particular to a high-efficiency nitrogen absorption peanut variety screening method and a culture observation device thereof.
Background
Peanut is the main oil crop and important economic crop in China, and the popularization and application of high-efficiency nitrogen absorption peanut varieties are the simplest and most effective method for improving the nitrogen fertilizer utilization rate. The conventional nitrogen efficient absorption and utilization variety identification method is comprehensive in field identification, but has a too long period, can not be rapidly screened and identified in a large quantity, and the test result is easily influenced by weather and management measures. In the indoor culture method, water culture (or culture medium culture) is needed for peanuts of various varieties in the screening process, seeds of materials to be tested are placed in a germination tray (culture medium) for culture, stress is often caused by hypoxia of the culture solution (culture medium), normal growth of the peanuts, particularly root systems, is influenced, and root system development is just critical for nitrogen absorption and utilization, so that the traditional culture method and device cannot achieve the due identification effect.
At present, the uniform oxygen supply to the root water area cannot be realized by the existing culture device, and the simple and rapid observation cannot be carried out by the existing culture device. Therefore, how to disclose a high-efficiency nitrogen absorption peanut variety screening method and a culture observing device, which reduce the identification period, simplify the screening method and avoid the difficulty of root system observation is a problem to be solved in the field.
Disclosure of Invention
In view of the above, the invention provides a high-efficiency nitrogen absorption peanut variety screening method and a culture observing device, which solve the problems that the existing peanut identifying method is overlong in period and cannot realize batch screening identification, and also solve the problems of nonuniform oxygen supply and difficult observation of the existing culture observing device.
In order to achieve the above purpose, the invention adopts the following technical scheme:
a screening method of peanut varieties capable of absorbing nitrogen efficiently comprises the steps of culturing peanut water for 12-15 days or culturing the peanut water in a culture medium for 20-25 days, detecting root system development conditions, calculating a root system development coefficient S, and determining the peanut varieties capable of absorbing nitrogen efficiently when the root system development coefficient S is greater than 1.5.
Preferably, the root system development coefficient S satisfies the formula: s=lt/Lc; wherein Lt is the total root system length of the treatment group, lc is the total root system length of the control group, the total nitrogen in the water or the culture medium of the treatment group is 23.1-28.8mg/kg, and the total nitrogen in the water or the culture medium of the control group is 43.3-46.1mg/kg;
the treatment group differed from the control group only by total nitrogen content.
Preferably, the culture conditions are: maintaining the illumination intensity of 18000-22000 Lux and the temperature of 28-32 ℃ for 15-17 hours every day; the illumination intensity in the rest time is 0Lux, and the temperature is 26-27 ℃.
The invention further aims to provide a culture observation device based on a high-efficiency nitrogen absorption peanut variety screening method, which comprises an incubator (1) and a controller (2), wherein the front end of the incubator (1) is rotatably connected with a transparent opening cover (6) through a hinge, a culture cylinder (7) is arranged at the inner side of the bottom end of the incubator (1), an air supplementing one-way valve (13) is fixedly connected with the inner side of the bottom end of the culture cylinder (7), a dispersing disc (14) is fixedly connected with the bottom end of the culture cylinder (7), the dispersing disc (14) is arranged at the upper side of the air supplementing one-way valve (13), a conical part (14 b) is fixedly connected with the bottom end of the dispersing disc (14), through holes (14 a) are formed in a penetrating manner up and down, oxygen emitted by the air supplementing one-way valve (13) can be dispersed through the dispersing disc (14), and the culture cylinder (7) is made of glass materials.
Preferably, a supply pump (3) is arranged on the outer side of the incubator (1), the output end of the supply pump (3) is communicated with the bottom end input end of the air supplementing one-way valve (13) through a pipeline, and the input end of the supply pump (3) can be communicated with an oxygen bottle through a supply valve and a pipeline interface.
Preferably, a second chute (11) is formed in the inner side of the bottom end of the incubator (1), a sliding block (4) is connected to the inner side of the second chute (11) in a sliding manner, the sliding block (4) is in an L-shaped arrangement, the rear end of the sliding block (4) is arranged at the rear side of the incubator (7), and a light supplementing lamp (5) for observing the incubator (7) is arranged at the upper side of the rear end of the sliding block (4);
an oil cavity (18) is formed in the inner side of the sliding block (4), and the inner side of the rear end of the oil cavity (18) is communicated with the input end of the hydraulic rod (16) through a connecting pipe (17).
Preferably, the light supplementing lamp (5) is rotatably arranged at the upper side of the rear end of the sliding block (4) through a hinge, the movable end of the hydraulic rod (16) is rotatably connected with the connecting rod (15) through the hinge, and the top end of the connecting rod (15) is rotatably connected with the lower side of the rear end of the light supplementing lamp (5) through the hinge.
Preferably, a piston (19) is slidably connected to the inner side of the oil cavity (18), and the lifting movement of the movable end of the hydraulic rod (16) is achieved through the forward and backward movement of the piston (19).
Preferably, the front end of the incubator (1) is slidably connected with a first chute (8), the inner side of the first chute (8) is slidably connected with a sealing plate (12), and the sealing of the second chute (11) is realized jointly through the sealing plate (12) and the front end of the sliding block (4).
Preferably, the front end of the sliding block (4) is fixedly connected with a push rod (10), the sliding block (4) is moved left and right by pulling the push rod (10), the front end of the piston (19) is fixedly connected with an operating rod (9), and the outer side of the operating rod (9) is in sliding connection with the inner side of the push rod (10);
the top of incubator (1) is fixedly connected with plant illumination lamp (20) that are used for the light filling.
Compared with the prior art, the invention has the following beneficial effects:
1. according to the invention, the peanut variety with high-efficiency nitrogen absorption is identified and screened indoors through the root system development condition in the seedling stage, and compared with the identification in the field full-growth stage, the experiment can be repeated in a short period, so that the identification period is greatly shortened; errors caused by weather, field management and the like can be eliminated, and the accuracy of identification is ensured; the invention is not limited by the field land, can realize batch identification and greatly improves the identification efficiency.
2. The method disclosed by the invention has the advantages of small seed consumption, direct application to early-generation material screening, acceleration of breeding process, improvement of breeding efficiency, reduction of breeding cost, laying of a foundation for cultivation and popularization of high-efficiency nitrogen absorption peanut varieties, and technical support for peanut weight-losing synergistic production.
3. According to the invention, the inner side of the bottom end of the incubator is provided with the incubator, the inner side of the bottom end of the incubator is fixedly connected with the air supplementing one-way valve, the bottom end of the incubator is fixedly connected with the dispersing disc, the dispersing disc is arranged on the upper side of the air supplementing one-way valve, the bottom end of the dispersing disc is fixedly connected with the conical part, the dispersing disc is penetrated up and down with through holes, oxygen emitted by the air supplementing one-way valve can be dispersed through the dispersing disc, the incubator is made of glass materials, under the arrangement, when the air supplementing one-way valve emits the air, the air firstly collides with the dispersing disc, and is dispersed outwards under the action of the conical part on the lower side of the dispersing disc, and the air continuously emits upwards through the through holes in the dispersing process, so that even oxygenation of culture medium in the incubator can be realized, and the oxygen can be pumped to an input port of the air supplementing one-way valve by the feeding pump.
4. According to the invention, the sliding block is arranged in an L shape, the rear end of the sliding block is arranged at the rear side of the culture cylinder, the upper side of the rear end of the sliding block is provided with the light supplementing lamp used for observing the culture cylinder, under the arrangement, the sliding block can be moved horizontally to the position of the sliding block by pulling the push rod, the sliding block drives the light supplementing lamp to move to the rear side of the culture cylinder to be observed, the light supplementing lamp can be conveniently and rapidly adjusted in the process of moving the push rod by increasing the brightness and increasing the accuracy of observation, during adjustment, a worker pushes the operating rod in sequence, the piston moves backwards to compress an oil cavity, hydraulic oil enters the inner side of the hydraulic rod through the connecting pipe to realize the rising of the movable end of the hydraulic rod, the movable end of the hydraulic rod drives the rear end of the light supplementing lamp to rise through the connecting rod, the front end of the light supplementing lamp descends, and during operation, and during the contrary, the light supplementing lamp is adjusted to an angle to be observed, the set plant irradiation lamp is used for realizing the environment simulating outdoor environment, and the influence of external variable factors on seeds is avoided
Drawings
FIG. 1 is a schematic view of the overall mounting structure of the present invention;
FIG. 2 is a schematic view showing an internal mounting structure at a culture drum of the present invention;
FIG. 3 is a schematic diagram of an elevational cross-sectional mounting structure of the present invention;
FIG. 4 is a schematic view of the mounting structure at the diverging disk of the present invention;
FIG. 5 is a schematic view of the mounting structure at an oil chamber of the present invention;
FIG. 6 is a development chart of peanut root system obtained in example 1 of the present invention, wherein a corresponds to the treatment group and b corresponds to the control group;
FIG. 7 is a root development diagram of peanut obtained in example 2 of the present invention, wherein a corresponds to the treatment group and b corresponds to the control group;
fig. 8 is a root development diagram of peanut obtained in example 3 of the present invention, a corresponds to the treatment group, and b corresponds to the control group.
In the figure: 1. an incubator; 2. a controller; 3. an oxygen supply pump; 4. a slide block; 5. a light supplementing lamp; 6. an opening cover; 7. a culture drum; 8. a first chute; 9. an operation lever; 10. a push rod; 11. a second chute; 12. a sealing plate; 13. an air supplementing one-way valve; 14. a diverging disk; 14a, through holes; 14b, a taper; 15. a connecting rod; 16. a hydraulic rod; 17. a connecting pipe; 18. an oil chamber; 19. a piston; 20. plant irradiation lamp.
Detailed Description
A screening method of peanut varieties capable of absorbing nitrogen efficiently comprises the steps of culturing peanut water for 12-15 days or culturing the peanut water in a culture medium for 20-25 days, detecting root system development conditions, calculating a root system development coefficient S, and determining the peanut varieties capable of absorbing nitrogen efficiently when the root system development coefficient S is greater than 1.5.
In the invention, the water culture time is preferably 13-14 days, and more preferably 13 days; the culture time of the medium is preferably 21 to 24 days, more preferably 23 days.
In the invention, the root system development coefficient S satisfies the formula: s=lt/Lc; wherein Lt is the total root system length of the treatment group, lc is the total root system length of the control group, and total nitrogen in water or culture medium of the treatment group is 23.1-28.8mg/kg, preferably 24-26 mg/kg, and more preferably 25mg/kg; the total nitrogen in the control water or the culture medium is 43.3-46.1mg/kg, preferably 44-45.5 mg/kg, and more preferably 45mg/kg.
In the present invention, the treatment group was different from the control group only in total nitrogen content.
In the present invention, the culture conditions are: the light intensity is 18000-22000 Lux, the temperature is 28-32 ℃ for 15-17 h, the light intensity is 19000-21000 Lux, the temperature is 29-31 ℃ for 16h, and the light intensity is 20000Lux and the light intensity is 30 ℃ for 16h; the illumination intensity in the rest time is 0Lux, and the temperature is 26-27 ℃, preferably 27 ℃.
The invention also provides a culture observation device for the efficient nitrogen absorption peanut variety screening method. Including incubator and controller, the front end of incubator is connected with transparent opening lid through the hinge rotation, the bottom inboard of incubator is provided with the culture dish, the inboard fixedly connected with air supplementing check valve of bottom of culture dish, the bottom fixedly connected with of culture dish diverges the dish, it sets up the upside at air supplementing check valve to diverge the dish, the bottom fixedly connected with toper portion of diverging the dish, it has the through-hole to link up from top to bottom to diverge the dish, the oxygen that air supplementing check valve was blown out can be realized dispersing through diverging the dish, the culture dish is made by glass material, under this kind of setting, when air supplementing check valve was blown out gas, gas first hit diverges the dish, disperses outside under the effect of diverging dish downside toper portion, and in dispersed in-process, gas continuous upwards goes out through the through-hole to realize the even oxygenation to the culture medium in the culture dish.
In the invention, the outer side of the incubator is provided with the feeding pump, the output end of the feeding pump is communicated with the bottom end input end of the air supplementing one-way valve through a pipeline, the input end of the feeding pump can be communicated with the oxygen bottle through a supply valve and a pipeline interface, and under the arrangement, the feeding pump can pump oxygen to the input port of the air supplementing one-way valve.
According to the invention, the inner side of the bottom end of the incubator is provided with the second chute, the inner side of the second chute is connected with the sliding block in a sliding manner, the sliding block is in an L-shaped arrangement, the rear end of the sliding block is arranged at the rear side of the incubator, the upper side of the rear end of the sliding block is provided with the light supplementing lamp for observing the incubator, under the arrangement, the sliding block drives the light supplementing lamp to move to the rear side of the incubator to be observed by stirring the push rod, the light supplementing lamp can conveniently and rapidly adjust the angle in the process of moving the push rod by increasing brightness, during adjustment, a worker pushes the operating rod in sequence, the piston moves backwards, the oil cavity is compressed, hydraulic oil enters the inner side of the hydraulic rod through the connecting pipe, the lifting of the movable end of the hydraulic rod is realized, the movable end of the hydraulic rod drives the rear end of the light supplementing lamp to lift through the connecting rod, the front end of the light supplementing lamp is lowered, and when the operation is opposite, so that the light supplementing lamp is adjusted to a required observation angle.
In the invention, an oil cavity is formed in the inner side of the sliding block, and the inner side of the rear end of the oil cavity is communicated with the input end of the hydraulic rod through a connecting pipe.
In the invention, the light supplementing lamp is rotatably arranged on the upper side of the rear end of the sliding block through the hinge, the movable end of the hydraulic rod is rotatably connected with the connecting rod through the hinge, and the top end of the connecting rod is rotatably connected with the lower side of the rear end of the light supplementing lamp through the hinge.
In the invention, the inner side of the oil cavity is connected with a piston in a sliding way, and the lifting movement of the movable end of the hydraulic rod is realized through the forward and backward movement of the piston.
In the invention, the front end of the incubator is slidably connected with the first chute, the inner side of the first chute is slidably connected with the sealing plate, the sealing of the second chute is realized through the sealing plate and the front end of the sliding block, and under the arrangement, the change of the gas content and the temperature of the incubator in a period of time can be reduced.
In the invention, the front end of the sliding block is fixedly connected with the push rod, the left-right movement of the sliding block is realized by stirring the push rod, the front end of the piston is fixedly connected with the operating rod, and the outer side of the operating rod is in sliding connection with the inner side of the push rod.
In the invention, the top end of the incubator is fixedly connected with the plant illumination lamp for light supplementing, and the plant illumination lamp is used for realizing the outdoor environment simulation and avoiding the influence of external variable factors on the cultivation of variety seeds.
The following description of the technical solutions in the embodiments of the present invention will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
In the device, the peanut seeds of Yuhua No. 37 are subjected to water culture for 13 days (the culture condition is set to be that the culture is kept for 16 hours at the temperature of 30 ℃ under the illumination intensity of 20000Lux every day, the illumination intensity of 0Lux at the temperature of 27 ℃ for the rest of time, the nitrogen content of a treatment group is 23.1mg/kg, the nitrogen content of a control group is 43.3 mg/kg.), the root system development condition is detected, the root system development diagram of the peanut is shown in fig. 6, wherein the fig. 6a corresponds to the treatment group, and the fig. 6b corresponds to the control group. The total root length L= 653.18cm of the treatment group, the total root length lc= 388.89cm of the control group, the root development coefficient S=1.68 and S greater than 1.5 are calculated, and the variety is a high-efficiency nitrogen absorption peanut variety.
Example 2
In the device, a culture medium of the Yuhua 22 peanut seeds is maintained for 25 days (the culture condition is set to be that the illumination intensity is 18000Lux and the temperature is 29 ℃ for 17 hours every day, the illumination intensity is 0Lux and the temperature is 26 ℃ for the rest time, the nitrogen content of a treatment group is 28.8mg/kg, the nitrogen content of a control group is 43.3mg/kg, and the culture medium is a cold junction resin culture medium), the root system development condition is detected, and a peanut root system development chart is shown in figure 7, wherein figure 7a corresponds to the treatment group, and figure 7b corresponds to the control group. The total root length lt= 470.46cm of the treatment group, the total root length lc= 299.58cm of the control group, the root development coefficient s=1.57 and S greater than 1.5 are calculated, and the variety is a high-efficiency nitrogen absorption peanut variety.
Example 3
In the device, peanut seeds of Yuhua 9719 are subjected to water culture for 15 days (culture conditions are set to be kept for 15 hours at the temperature of 31 ℃ under the illumination intensity of 22000Lux every day, the illumination intensity of 0Lux at the temperature of 27 ℃ for the rest time, the nitrogen content of a treatment group is 23.1mg/kg, the nitrogen content of a control group is 43.3 mg/kg.), the root system development condition is detected, and a peanut root system development chart is shown in fig. 8, wherein the fig. 8a corresponds to the treatment group, and the fig. 8b corresponds to the control group. The total root system length lt= 244.19 of the treatment group, the total root system length lc= 206.70cm of the control group, the root system development coefficient s=1.18 and S less than 1.5 is calculated, and the variety is a non-efficient nitrogen absorption peanut variety.
In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (7)
1. A screening method of peanut varieties capable of absorbing nitrogen efficiently is characterized in that the peanut varieties are cultivated for 12-15 days or cultivated in a culture medium for 20-25 days, the root system development condition is detected, the root system development coefficient S is calculated, and the root system development coefficient S is greater than 1.5, and then the peanut varieties capable of absorbing nitrogen efficiently are identified;
the root system development coefficient S satisfies the formula: s=lt/Lc; wherein Lt is the total root system length of the treatment group, lc is the total root system length of the control group, the total nitrogen in the water or the culture medium of the treatment group is 23.1-28.8mg/kg, and the total nitrogen in the water or the culture medium of the control group is 43.3-46.1mg/kg; the treatment group and the control group are different only in total nitrogen content; the high-efficiency nitrogen absorption peanut variety screening is carried out by adopting a culture observation device;
cultivate observation device including incubator (1) and controller (2), the front end of incubator (1) is connected with transparent opening lid (6) through the hinge rotation, the bottom inboard of incubator (1) is provided with cultivates section of thick bamboo (7), the inboard fixedly connected with air make-up check valve (13) of bottom of cultivateing section of thick bamboo (7), the bottom fixedly connected with of cultivateing section of thick bamboo (7) diverges dish (14), the setting of diverged dish (14) is in the upside of air make-up check valve (13), the bottom fixedly connected with toper portion (14 b) of diverged dish (14), it has through-hole (14 a) to link up from top to bottom to diverge dish (14), the oxygen that air make-up check valve (13) emit can be realized dispersing through divergently dish (14), cultivate section of thick bamboo (7) by the glass material.
2. The method for screening peanut varieties with high nitrogen absorption efficiency according to claim 1, wherein the culture conditions are as follows: the illumination intensity is 18000-22000 Lux and the temperature is 28-32 ℃ for 15-17 h every day; the illumination intensity in the rest time is 0Lux, and the temperature is 26-27 ℃.
3. The efficient nitrogen absorption peanut variety screening method according to claim 1, wherein a supply pump (3) is arranged on the outer side of the incubator (1), the output end of the supply pump (3) is communicated with the bottom end input end of the air supplementing one-way valve (13) through a pipeline, and the input end of the supply pump (3) is communicated with an oxygen bottle through a supply valve and a pipeline interface.
4. The efficient nitrogen absorption peanut variety screening method according to claim 1 or 3, characterized in that a second chute (11) is formed in the inner side of the bottom end of the incubator (1), a sliding block (4) is connected to the inner side of the second chute (11) in a sliding mode, the sliding block (4) is in an L-shaped arrangement, the rear end of the sliding block (4) is arranged at the rear side of the incubator (7), and a light supplementing lamp (5) for observing the incubator (7) is arranged at the upper side of the rear end of the sliding block (4); an oil cavity (18) is formed in the inner side of the sliding block (4), and the inner side of the rear end of the oil cavity (18) is communicated with the input end of the hydraulic rod (16) through a connecting pipe (17);
the light supplementing lamp (5) is rotatably arranged on the upper side of the rear end of the sliding block (4) through a hinge, the movable end of the hydraulic rod (16) is rotatably connected with a connecting rod (15) through the hinge, and the top end of the connecting rod (15) is rotatably connected with the lower side of the rear end of the light supplementing lamp (5) through the hinge.
5. The efficient nitrogen absorbing peanut variety screening method according to claim 4, wherein a piston (19) is slidably connected to the inner side of the oil cavity (18), and lifting movement of the movable end of the hydraulic rod (16) is achieved through forward and backward movement of the piston (19).
6. The efficient nitrogen absorbing peanut variety screening method according to claim 5, wherein a first chute (8) is slidably connected to the front end of the incubator (1), a sealing plate (12) is slidably connected to the inner side of the first chute (8), and sealing of a second chute (11) is achieved jointly through the sealing plate (12) and the front end of the sliding block (4).
7. The efficient nitrogen absorption peanut variety screening method according to claim 6 is characterized in that a push rod (10) is fixedly connected to the front end of the sliding block (4), the sliding block (4) is moved left and right by pulling the push rod (10), an operating rod (9) is fixedly connected to the front end of the piston (19), and the outer side of the operating rod (9) is in sliding connection with the inner side of the push rod (10); the top of incubator (1) is fixedly connected with plant illumination lamp (20) that are used for the light filling.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
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| CN202210702156.9A CN114982616B (en) | 2022-06-20 | 2022-06-20 | Efficient nitrogen absorption peanut variety screening method and culture observation device |
| PCT/CN2023/100620 WO2023246628A1 (en) | 2022-06-20 | 2023-06-16 | Method for screening peanut variety capable of efficient nitrogen absorption, and culture observation device |
| ZA2023/06350A ZA202306350B (en) | 2022-06-20 | 2023-06-19 | Method for screening peanut variety with high nitrogen absorption efficiency and culture observation apparatus therefor |
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| CN116965286B (en) * | 2023-09-25 | 2023-12-08 | 上海永大菌业有限公司 | Ventilation heat recovery type constant temperature edible fungi planting equipment |
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| WO2023246628A1 (en) | 2023-12-28 |
| CN114982616A (en) | 2022-09-02 |
| ZA202306350B (en) | 2024-01-31 |
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