CN106358785A - Method for screening low-nitrogen-tolerant peppers at seedling stage - Google Patents
Method for screening low-nitrogen-tolerant peppers at seedling stage Download PDFInfo
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- CN106358785A CN106358785A CN201610736264.2A CN201610736264A CN106358785A CN 106358785 A CN106358785 A CN 106358785A CN 201610736264 A CN201610736264 A CN 201610736264A CN 106358785 A CN106358785 A CN 106358785A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Abstract
The invention belongs to the technical field of pepper breeding, and relates to a method for screening low-nitrogen tolerant pepper seedlings, and the method is used for solving the technical problem of providing a novel choice for screening a low-nitrogen-tolerant pepper species resource at the seedling stage. The method for screening low-nitrogen-tolerant peppers at a seedling stage specifically comprises the following steps of: a, culturing; b, collecting data; and c, calculating. The method is fewer in determination indexes, simple and easy to implement.
Description
Technical field
The invention belongs to pepper breeding technical field, it is related to a kind of resistance to low nitrogen screening technique of chilli seedling.
Background technology
Nitrogen is the important nutrient of growth and development of plants and yield composition, and no matter existing substantial amounts of research shows plant
It is all to exist to N-uptake and use efficiency effect between different kinds or between same kind of different cultivars (or strain)
The difference of rate, that is, the ability of resistance to Low nitrogen stress is different.
Fructus Capsici is vegetable very popular all over the world, containing abundant vitamin c, beta-carotene, Folic Acid, magnesium and potassium,
Capsaicin energy appetite stimulator in Fructus Capsici, also has antiinflammatory and antioxidation, contributes to reducing the danger such as heart disease and tumor,
Fructus Capsici plays extremely important effect in food and drink and health care industry.China is the first Fructus Capsici manufacturing country and master in the world
Want country of consumption, be also one of most country of Fructus Capsici outlet.However, in order to improve yield, being continuously increased the administration of nitrogenous fertilizer, not only
Increased agriculture production cost, reduce Quality of Capsicum, environment be also result in and have a strong impact on.Therefore, screen and cultivate resistance to low
The strong kind of nitrogen ability, is to improve soil depletion ground Fructus Capsici nitrogen utilization efficiency and yield, reduces the important way of nitrogenous fertilizer usage amount
Footpath.At present both at home and abroad there is not yet the research of the resistance to low nitrogen screening technique of Fructus Capsici is reported.
Content of the invention
The technical problem to be solved in the present invention is to provide a kind of new selection for screening the Hot Pepper Seedling of resistance to low nitrogen.
The technical scheme is that a kind of resistance to low nitrogen screening technique of chilli seedling, comprise the steps:
A, culture: the seed of germination is placed in culture in nutritional solution by floating plate；Nutritional solution sets and normally supplies nitrogen and low nitrogen
Two process, it is the 20% of normal process containing Different Nitrogen Concentration that low nitrogen processes nutrient solution used, each process nutritional solution ph be 6.2～
6.4；Change within every 3～4 days one time of nutrition liquid, 25～28 DEG C of cultivation temperature, daily illumination 12～14h, intensity of illumination 4600～
6000lx, relative air humidity 70%～80%；
B, collect data: after processing 28-30 days, measure respectively the leaf area of every plant, aerial partss dry weight, root dry weight,
Aerial partss nitrogen content, root nitrogen content, and according to below equation calculate individual plant resistance to low nitrogen index: total dry weight, root/shoot ratio,
Upper part nitrogen accumulation, root nitrogen accumulation, individual plant nitrogen accumulation；Total dry weight=aerial partss dry weight+root dry weight, root/shoot ratio=
Root dry weight/aerial partss dry weight, aerial partss nitrogen accumulation=ground dry weight × aerial partss nitrogen content, root nitrogen accumulation=root
Dry weight × root nitrogen content, individual plant nitrogen accumulation=aerial partss nitrogen accumulation+root nitrogen accumulation；
C, calculating: calculate the resistance to low nitrogen index x of each single index according to below equation, with the resistance to low nitrogen index x of each index
For variable, using lines by fuzzy administering function, overall merit is carried out to the resistance to low nitrogen ability of each kind；
The nitrogen that normally supplies that the low nitrogen of x=index processes measured value/index processes measured value
pij=(xij-xjmin)/(xjmax-xjmin) (i=1,2 ... ... n；J=1,2 ... ... n)
Above calculating is related to 8 indexs, comprising: leaf area, aerial partss dry weight, root dry weight, total dry weight, root/shoot ratio,
Aerial partss nitrogen accumulation, root nitrogen accumulation, individual plant nitrogen accumulation.pijFor the membership function value of the i-th kind jth index, 0≤
pij≤ 1, xijFor the resistance to low nitrogen coefficient of the i-th kind jth index, xjminFor the minima of jth index, xjmaxFor jth index
Big value, wjFor weight, cvjFor the coefficient of variation of jth index, diFor the i-th kind comprehensively resistance to low nitrogen index；diValue is bigger, shows the
The resistance to low nitrogen ability of i kind is stronger.
Preferably, the operation of the germination described in step a is as follows: selects full consistent and no disease and pests harm seed, passes through
Hot water treatment of seeds, wash by rubbing with the hands after draining away the water, seed is uniformly put in the culture dish filling filter paper, add distilled water, keep culture
In ware, filter paper moistens, and is placed in growth cabinet, 25～28 DEG C, germinates under dark condition.
Preferably, after the seed after germinateing in step a is cleaned with distilled water, a point individual plant moves on on black floating plate.
Specifically, floating plate specification 16.5cm × 10.5cm in step a, making a call to 15 apertures on each floating plate is
The circular hole of 0.5cm, laterally makes a call to 3 rounds, often arranges 5 holes, and Kong Yukong is spaced 2.0cm.
Specifically, when in step a, seed just moves on to floating plate, the frivolous soft absorbent paper of 2 layers of each Kong Kesai, seed is certainly
So it is placed in absorbent paper, to play the supporting role to immature seed, absorbent paper can be passed through by nature after the elongation of seedling root,
With nutritional solution directly contact.
Preferably, with distilled water, plant root residual nutritional solution is cleaned up before changing nutritional solution in step a.
The resistance to low nitrogen character of kind in order to verify screening further, uses the different capsicum variety of the resistance to low nitrogen level filtering out
Carry out field experiment, if 2 process of normal nitrogen fertilizer application and not nitrogen fertilizer application, measure individual plant fresh material yield after maturation and dry produces
Amount, calculates its resistance to low nitrogen index (measured value that measured value/nitrogen fertilizer application that nitrogen fertilizer application is not processed is processed), capsicum variety is carried out resistance to
Low nitrogen evaluation and identification, resistance to low nitrogen index height is that resistance to low nitrogen level is high.Also analyze the resistance to low nitrogen of period of maturation different output index simultaneously
Index and the dependency of the resistance to low nitrogen index of seedling stage each physical signs, verify the effectiveness of seedling screening further.
Beneficial effects of the present invention: the present invention utilizes ciltivating process to cultivate pepper seedling, there is provided a homogeneous root system
Growing environment；Cultivation temperature, illumination condition, humidity are all controlled；Floating plate in culture, culture box specification is fixed, space between plants
From fixation.Therefore, it is possible to ensure repeatability and the accuracy of the selection result.In addition, only needing practical measurement leaf area, overground part
Dry weight, root dry weight, ground nitrogen content, 5 indexs of root nitrogen content, other indexs are all to be calculated by these indexs,
Therefore, testing index is few, and method is simply easily implemented.
Brief description
Fig. 1 capsicum variety period of maturation resistance to low nitrogen difference
Specific embodiment
Embodiment adopts the inventive method to screen resistance to low nitrogen Hot Pepper Seedling
(1) select full consistent and 25 parts of Fructus Capsici materials of no disease and pests harm seeds, after hoting water treatment of seeds, wash by rubbing with the hands and drain
Moisture, seed is uniformly put in the culture dish filling two layers of filter paper, adds distilled water, keeps filter paper in culture dish to moisten, puts
In growth cabinet, germinate under 25～28 DEG C of dark conditions.
(2) after cleaning the seed after germinateing with distilled water, a point individual plant moves on on black sponge floating plate, then will float
Plate is suspended in the uncovered culture box equipped with nutritional solution that size is mated with floating plate, with black plastic bag, box body is hidden
Light.
(3) floating plate specification 16.5cm × 10.5cm, makes a call to the circular hole that 15 apertures are 0.5cm on each floating plate, horizontal
To making a call to 3 rounds, often arrange 5 holes, Kong Yukong is spaced 2.0cm.
(4) the long 17.5cm of box, wide 11.5cm, high 7cm are cultivated.
(5) when seed just moves on to floating plate, the frivolous soft absorbent paper of 2 layers of each Kong Kesai, seed is placed on suction naturally
On water paper, to play the supporting role to immature seed, absorbent paper can be passed through by nature after the elongation of seedling root, straight with nutritional solution
Contact.
(6) adopt mountain rugged Fructus Capsici nutritional solution, formula is as shown in table 1.If normally supplying nitrogen and two process of low nitrogen, wherein low nitrogen
Process nutrient solution used containing ca (no3)2·4h2O, kno3, nh4h2po4Three kinds of compound concentrations containing nitrogen are normal process
20%, and supplement interpolation k in low nitrogen treatment group2so4、kh2po4、cacl2, thus making up reduction ca in low nitrogen treatment group
(no3)2·4h2o、kno3、nh4h2po4Consumption leads to k, the shortage of this 3 plant growth key element of p, ca.Each process nutrition
Liquid ph is 6.2～6.4.
Table 1 mountain rugged Fructus Capsici nutrient solution prescription
| Reagent | Consumption (mg/l) |
| ca(no3)2·4h2o | 354 |
| kno3 | 607 |
| nh4h2po4 | 96 |
| mgso4·7h2o | 185 |
| feso4·7h2o | 27.85 |
| na2edta | 37.25 |
| h3bo3 | 2.86 |
| znso4·7h2o | 0.22 |
| mnso4·4h2o | 2.13 |
| cuso4·5h2o | 0.08 |
| (nh4)6mo7o24·4h2o | 0.02 |
(7) add 500ml nutritional solution every time in each culture box, on every Mondays, Friday changes one time of nutrition liquid.Change nutritional solution
Plant root residual nutritional solution is cleaned up by front distilled water
(8) 25～28 DEG C of cultivation temperature, daily illumination 12～14h, intensity of illumination 4600～6000lx, relative air humidity
70%～80%.
(9) every kind is every kind of processes culture 3 box plant, is that 15 plant are repeated for 1 with 1 box.
(10), after processing 28～30 days, the leaf area of each box plant, overground part dry weight, root dry weight, on the ground are measured respectively
Portion's nitrogen content, root nitrogen content, and total dry weight, root/shoot ratio, overground part nitrogen accumulation, the accumulation of root nitrogen are calculated according to below equation
Amount, individual plant nitrogen accumulation.
(11) total dry weight=overground part dry weight+root dry weight
(12) root/shoot ratio=root dry weight/overground part dry weight
(13) overground part nitrogen accumulation=ground dry weight × overground part nitrogen content
(14) root nitrogen accumulation=root dry weight × root nitrogen content
(15) individual plant nitrogen accumulation=overground part nitrogen accumulation+root nitrogen accumulation
(16) disposal data, calculates individual plant meansigma methodss, and calculates the resistance to low nitrogen index of each single index according to below equation
x.
(17) the low nitrogen of x=index processes the normal of measured value/index and supplies nitrogen process measured value
(18) with the resistance to low nitrogen index x of each index as variable, using the resistance to low nitrogen energy to each kind for the lines by fuzzy administering function
Power carries out overall merit.
(19)pij=(xij-xjmin)/(xjmax-xjmin) (i=1,2 ... ... n；J=1,2 ... ... n)
(20)
(21)
(22) calculate more than and be related to 8 indexs, comprising: leaf area, aerial partss dry weight, root dry weight, total dry weight, root cap
Than, aerial partss nitrogen accumulation, root nitrogen accumulation, individual plant nitrogen accumulation.
(23)pijMembership function value (0≤p for the i-th kind jth indexij≤ 1), xijResistance to for the i-th kind jth index
Low nitrogen coefficient, xjminFor the minima of jth index, xjmaxFor the maximum of jth index, wjFor weight, cvjChange for jth index
Different coefficient, diFor the i-th kind comprehensively resistance to low nitrogen index.
(24)diValue is bigger, shows that the resistance to low nitrogen ability of the i-th kind is stronger.As can be seen from Table 2 in 25 parts of Fructus Capsici materials
Resistance to low nitrogen ability the strongest for early spring, resistance to low nitrogen ability the weakest for 1305.
The different resistance to low nitrogen ability fuzzy membership functions aggregative index of pepper seedling of table 2
(25) select to determine that three different capsicum varieties of resistance to low nitrogen level carry out field experiment in seedling stage.Test location
Soil property is soil, containing organic 41.0g/kg, full nitrogen 2.36g/kg, available nitrogen 50.46mg/kg, moisture 3.1%, ph 7.52.
Test adopts split block design, and primary area is kind, respectively early spring, 834-2-1 and j1364；Secondary area is soil fertility quality, if normally applying
Nitrogenous fertilizer (carbamide 0.1kg should be applied by 1000 kilograms of every cells of calculating of per mu yield) and not 2 process of nitrogen fertilizer application；3 times repetition, totally 9 little
Area, each plot area is about 5.6m2(long 4m, wide 1.4m), individual plant is cultivated, and every cell plants 16 plant, spacing in the rows about 0.5m.
Period of maturation plucked a fruit every 2 weeks, plucked four times altogether, was cleaned with tap water, 105 DEG C are killed after measuring fresh material yield every time
After blue or green 30min, dry at 70 DEG C to constant weight, measure dry matter production.
(26) period of maturation fresh material yield and the resistance to low nitrogen index (mensure that nitrogen fertilizer application is not processed of dry matter production are calculated
The measured value that value/nitrogen fertilizer application is processed).According to the resistance to low nitrogen index of Fructus Capsici period of maturation fresh material yield and dry matter production, judge
The resistance to low nitrogen level of capsicum variety, resistance to low nitrogen index is higher, and resistance to low nitrogen level is higher, it will be seen from figure 1 that early spring in three materials
Resistance to low nitrogen level highest, is secondly respectively 834-2-1 and j1364, consistent with the qualification result in seedling stage.
(27) dependency of analysis period of maturation resistance to low nitrogen index and seedling stage resistance to low nitrogen index, establish period of maturation resistance to low nitrogen with
The dependency of seedling stage resistance to low nitrogen.Table 3 display period of maturation fresh material yield is in extremely significantly correlated with leaf area at the seedling stage, with overground part
Dry weight, root dry weight, total dry weight, overground part nitrogen accumulation, root nitrogen accumulation and individual plant nitrogen accumulation are in significantly correlated, dry
Yield, in addition to these dependencys, is in extremely significantly correlated also with seedling stage individual plant nitrogen accumulation.Therefore, can using seedling screening system
Extensive Preliminary Identification is carried out with low nitrogen level resistance to Fructus Capsici effectively.
Table 3 period of maturation and seedling stage resistance to low nitrogen correlation analysiss
| Type | Leaf area | Overground part dry weight | Root dry weight | Total dry weight | Root/shoot ratio | Overground part nitrogen accumulation | Root nitrogen accumulation | Individual plant nitrogen accumulation |
| Fresh material yield | 0.866** | 0.720* | 0.782* | 0.756* | 0.418 | 0.754* | 0.786* | 0.787* |
| Dry matter production | 0.866** | 0.704* | 0.722* | 0.732* | 0.321 | 0.775* | 0.721* | 0.798** |
*Represent significantly correlated (p ＜ 0.05),**Represent significantly correlated (p ＜ 0.01).
Claims (6)
1. a kind of resistance to low nitrogen screening technique of chilli seedling it is characterised in that: comprise the steps:
A, culture: the seed of germination is placed in culture in nutritional solution by floating plate；Nutritional solution sets and normally supplies nitrogen and two, low nitrogen
Process, it is the 20% of normal process containing Different Nitrogen Concentration that low nitrogen processes nutrient solution used, each nutritional solution ph that processes is 6.2～6.4；Often
Change within 3～4 days one time of nutrition liquid, 25～28 DEG C of cultivation temperature, daily illumination 12～14h, intensity of illumination 4600～6000lx, sky
Gas relative humidity 70%～80%；
B, collect data: after processing 28-30 days, measure the leaf area of every plant, aerial partss dry weight, root dry weight, on the ground respectively
Part nitrogen content, root nitrogen content, and the resistance to low nitrogen index of individual plant: total dry weight, root/shoot ratio, overground part is calculated according to below equation
Divide nitrogen accumulation, root nitrogen accumulation, individual plant nitrogen accumulation；Total dry weight=aerial partss dry weight+root dry weight, root/shoot ratio=root is done
Weight/aerial partss dry weight, aerial partss nitrogen accumulation=ground dry weight × aerial partss nitrogen content, root nitrogen accumulation=root dry weight
× root nitrogen content, individual plant nitrogen accumulation=aerial partss nitrogen accumulation+root nitrogen accumulation；
C, calculating: calculate the resistance to low nitrogen index x of each single index according to below equation, with the resistance to low nitrogen index x of each index for becoming
Amount, carries out overall merit using lines by fuzzy administering function to the resistance to low nitrogen ability of each kind；
The nitrogen that normally supplies that the low nitrogen of x=index processes measured value/index processes measured value
pij=(xij-xjmin)/(xjmax-xjmin) (i=1,2 ... ... n；J=1,2 ... ... n)
Above calculating is related to 8 indexs, comprising: leaf area, aerial partss dry weight, root dry weight, total dry weight, root/shoot ratio, on the ground
Part nitrogen accumulation, root nitrogen accumulation, individual plant nitrogen accumulation；pijFor the membership function value of the i-th kind jth index, 0≤pij≤
1, xijFor the resistance to low nitrogen coefficient of the i-th kind jth index, xjminFor the minima of jth index, xjmaxFor the maximum of jth index,
wjFor weight, cvjFor the coefficient of variation of jth index, diFor the i-th kind comprehensively resistance to low nitrogen index；diValue is bigger, shows the i-th kind
Resistance to low nitrogen ability stronger.
2. the resistance to low nitrogen screening technique of chilli seedling as claimed in claim 1 it is characterised in that: the behaviour germinateed described in step a
Make as follows: select full consistent and no disease and pests harm seed, through hoting water treatment of seeds, wash by rubbing with the hands after draining away the water, seed is uniformly put
Enter to fill in the culture dish of filter paper, add distilled water, keep filter paper in culture dish to moisten, be placed in growth cabinet, 25～28 DEG C,
Germinate under dark condition.
3. the resistance to low nitrogen screening technique of chilli seedling as claimed in claim 1 or 2 it is characterised in that: the kind after germinateing in step a
After son is cleaned with distilled water, a point individual plant moves on on black floating plate.
4. the resistance to low nitrogen screening technique of chilli seedling as claimed in claim 3 it is characterised in that: floating plate specification in step a
16.5cm × 10.5cm, makes a call to the circular hole that 15 apertures are 0.5cm on each floating plate, laterally makes a call to 3 rounds, often arranges 5 holes, hole
It is spaced 2.0cm with hole.
5. the resistance to low nitrogen screening technique of chilli seedling as claimed in claim 4 it is characterised in that: in step a, seed moves on to floating
During plate, the frivolous soft absorbent paper of 2 layers of each Kong Kesai, seed is placed in absorbent paper naturally, to play to immature seed
Supporting role, can pass through absorbent paper by nature, with nutritional solution directly contact after the elongation of seedling root.
6. the resistance to low nitrogen screening technique of chilli seedling as claimed in claim 1 it is characterised in that: before changing nutritional solution in step a
With distilled water, plant root residual nutritional solution is cleaned up.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107439179A (en) * | 2017-08-02 | 2017-12-08 | 中国林业科学研究院亚热带林业研究所 | The screening technique of nutritional factors needed for cultivation of container seedling and the container seedling obtained using it |
| CN108184633A (en) * | 2018-01-10 | 2018-06-22 | 上海市农业科学院 | A kind of screening technique of resistance to low nitrogen barley |
| CN114982616A (en) * | 2022-06-20 | 2022-09-02 | 河南省农业科学院经济作物研究所 | Efficient nitrogen absorption peanut variety screening method and culture observation device |
| CN115508509A (en) * | 2022-09-16 | 2022-12-23 | 吉林省农业科学院 | Evaluation method for low-nitrogen resistance of soybean in seedling stage |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107439179A (en) * | 2017-08-02 | 2017-12-08 | 中国林业科学研究院亚热带林业研究所 | The screening technique of nutritional factors needed for cultivation of container seedling and the container seedling obtained using it |
| CN108184633A (en) * | 2018-01-10 | 2018-06-22 | 上海市农业科学院 | A kind of screening technique of resistance to low nitrogen barley |
| CN114982616A (en) * | 2022-06-20 | 2022-09-02 | 河南省农业科学院经济作物研究所 | Efficient nitrogen absorption peanut variety screening method and culture observation device |
| CN114982616B (en) * | 2022-06-20 | 2024-02-02 | 河南省农业科学院经济作物研究所 | Efficient nitrogen absorption peanut variety screening method and culture observation device |
| CN115508509A (en) * | 2022-09-16 | 2022-12-23 | 吉林省农业科学院 | Evaluation method for low-nitrogen resistance of soybean in seedling stage |
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Inventor after: Wang Chunping Inventor after: Li Yifei Inventor after: Lin Qing Inventor after: Huang Qizhong Inventor after: Zhang Shicai Inventor after: Wu Hong Inventor after: Lei Kairong Inventor after: Huang Renzhong Inventor after: Lv Zhonghua Inventor after: Jiang Xiaoying Inventor before: Wang Chunping Inventor before: Lin Qing Inventor before: Huang Qizhong Inventor before: Zhang Shicai Inventor before: Wu Hong Inventor before: Lei Kairong Inventor before: Jiang Xiaoying Inventor before: Li Yifei |
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| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170201 |