CN102106247A - Method for identifying and screening barley low-nitrogen resistant property - Google Patents
Method for identifying and screening barley low-nitrogen resistant property Download PDFInfo
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- CN102106247A CN102106247A CN200910200611XA CN200910200611A CN102106247A CN 102106247 A CN102106247 A CN 102106247A CN 200910200611X A CN200910200611X A CN 200910200611XA CN 200910200611 A CN200910200611 A CN 200910200611A CN 102106247 A CN102106247 A CN 102106247A
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Abstract
The invention provides a method for screening and identifying a barley low-nitrogen resistant property. The method comprises the following steps of: screening and identifying a low-nitrogen resistant property in a seedling stage by a solution culture method; screening and identifying the low-nitrogen resistant property in a mature stage by a field experiment; and evaluating the barley low-nitrogen resistant property effectively by establishing the correlativity between the seedling stage and the mature stage, so that the barley low-nitrogen resistant property can be screened and identified quickly and effectively during the seedling stage. The implementation method provided by the invention is simple, a laboratory part is precise and controllable, and a field part is real and accurate; thus, results are effective and reliable.
Description
Technical field
The invention belongs to the barley breeding technical field, relate to the anti-low nitrogen character screening and the evaluation of seed as the barley of modes of reproduction.
Background technology
Barley is a primary raw material of making beer, also is good feed.In recent years, along with the fast development of China's brewing industry, increasing to the demand of malting barley.Nitrogen is the essential nutritive element of plant growth, in order to have guaranteed high crop yield and stable yields, and just must a large amount of applied nitrogens.In recent years, a large amount of uses along with nitrogenous fertilizer, when promoting crop yield, stable yields, many drawbacks have also been brought, as economic benefit decline, utilization rate of nitrogen fertilizer reduction, environmental pollution or the like, not only caused enormous economic loss, and severe contamination environment, human health has been constituted potential threat.It is 10%-12% that malting barley requires the top grade standard of grain protein content, but often increases amount of application of nitrogen fertilizer on producing in order to devote exclusive attention to output or fertility middle and later periods heavy dressing is topdressed, and grain protein content exceeds standard as a result, thereby has reduced the brewing quality of barley.Therefore, screen and cultivate anti-low nitrogen, the efficient barley new varieties of nitrogen will be the desirable approach that addresses these problems.
From present research means, the method that combines in the seedling stage and the time of infertility is generally adopted in the screening of anti-low nitrogen, investigates different indexs by water culture test, pot experiment and field trial, thereby estimates the anti-low nitrogen of kind.Field test results is more credible, but often is subjected to the restriction of room and time; And water culture test and pot experiment just can be carried out rapid screening to large batch of genotype in early days with regard to some morphology and physical signs, and condition of culture can carry out repetition like clockwork, but its credibility remains to be discussed.Also utilized and anti-low nitrogen studies about barley nitrogen is efficient in the past, what have has just made desk study by pot-culture method to the hereditary effect of barley nitrogen in seedling stage physiology availability, and to the anti-low nitrogen of barley in seedling stage has been carried out preliminary evaluation and screening, the field trial of passing through that has has been studied low nitrogen and has been coerced down the genetic variation that different genotype barley maturing stage nitrogen utilizes correlated traits and output.But these all combine barley anti-low nitrogen correlated traits in seedling stage and yield traits anti-low nitrogen is not estimated and screened.Therefore adopt the water culture test to study the anti-low nitrogen of barley in conjunction with the method for field trial, both can compare the difference of each genotype of barley in seedling stage between normally confession nitrogen and low nitrogen are coerced, again can comparative maturity phase each genotype of barley in fertilising with the difference between not applying fertilizer, taken into account the authenticity of characteristics such as aquicultural simple, good reproducibility and field trial simultaneously, guarantee for the anti-low nitrogen of scientific evaluation barley provides preferably, thereby also lay the foundation for barley anti-fast and effectively low nitrogen screening in seedling stage.
This area need disclose the anti-low nitrogen kind material of being covered for a long time of barley by means fast and effectively on producing, excavate anti-low nitrogen germ plasm resource in the barley germplasm storehouse, to obtain with seed as anti-low nitrogen proterties production kind of the barley of modes of reproduction or anti-low nitrogen germ plasm resource, thereby can in less fertilising cultivation mode or on the barren soil of China, plant production, to improve economy and environmental benefit, and utilize land resources widely, the more anti-low nitrogen genetic germplasm resource of screening also can be the anti-low nitrogen breeding service of the further barley of China.
Summary of the invention
The present invention is directed to the deficiency of anti-low nitrogen screening of barley and evaluation aspect, provide a kind of new anti-low nitrogen screening of barley and the method for identifying, and seedling stage, screening conditions were easy to unified control, were not subjected to the restriction of extraneous natural conditions, and field trial is more convincing, guarantees the result accurately and reliably.
Therefore, first aspect present invention provides anti-low nitrogen screening of a kind of barley and the method for identifying, this method comprises:
1) use water culture liquid to cultivate the barley wheat seeding, if low nitrogen and normal for 2 processing of nitrogen, about 4-5 receives seedling after week, to seedling stage biology and relevant physiological index investigate, described physical signs comprises nitrogen physiology availability, individual plant amount of nitrogen sucking, overground part dry weight, plant height and tiller number, carries out anti-low nitrogen evaluation in seedling stage and screening;
2) carry out field experiment with identical kind at Mai Zuoqu, establish normal nitrogen fertilizer application and not 2 processing of nitrogen fertilizer application, ripe back is surveyed to produce barley is carried out anti-low nitrogen evaluation and evaluation;
3) set up the correlation of seedling stage and the anti-low nitrogen of maturing stage barley, establish the screening index in seedling stage, thereby the anti-low nitrogen of barley is screened in seedling stage.
In a preferred embodiment, the nitrogen concentration scope of described low nitrogen is 0.1-0.8mmol/L, and normally the nitrogen concentration scope for nitrogen is 1-3mmol/L.
In another preferred embodiment, the nitrogen concentration scope of described low nitrogen is 0.2-0.6mmol/L, and normally the nitrogen concentration scope for nitrogen is 1.5-2.5mmol/L.
In a preferred embodiment, the pH of described water culture liquid is 5.5-6.0.
The pH of the liquid of water culture in a preferred embodiment, is 5.6-5.8.
In a preferred embodiment, described nitrogenous source is with NH
4NO
3Form is supplied in the water culture liquid.
In a preferred embodiment, described seedling stage, screening index was a nitrogen physiology availability and/or tiller number in seedling stage.
In a preferred embodiment, screening obtains the wheat seeding of nitrogen physiology availability relative value in seedling stage 〉=1.8, tiller number relative value 〉=0.8, it is anti-low nitrogen barley wheat seeding, and wherein, described seedling stage, nitrogen physiology availability relative value was coerced character value under the confession nitrogen of character value down/normally for low nitrogen.
The method that the present invention also provides a kind of anti-low nitrogen barley screening on the other hand and identifies, this method comprises:
Use water culture liquid is cultivated the barley wheat seeding, establishes low nitrogen and normally supplies 2 processing of nitrogen, and 4-5 receives seedling after week, measures nitrogen physiology availability and tiller number;
Wherein, the wheat seeding of nitrogen physiology availability relative value 〉=1.8, tiller number relative value 〉=0.8 is elected anti-low nitrogen barley as,
Wherein, described seedling stage, nitrogen physiology availability relative value was coerced character value under the confession nitrogen of character value down/normally for low nitrogen.
In a preferred embodiment, the nitrogen concentration scope of described low nitrogen is 0.1-0.8mmol/L, and normally the nitrogen concentration scope for nitrogen is 1-3mmol/L.
In another preferred embodiment, the nitrogen concentration scope of described low nitrogen is 0.2-0.6mmol/L, and normally the nitrogen concentration scope for nitrogen is 1.5-2.5mmol/L.
In a preferred embodiment, the pH of described water culture liquid is 5.5-6.0.
The pH of the liquid of water culture in a preferred embodiment, is 5.6-5.8.
In a preferred embodiment, described nitrogenous source is with NH
4NO
3Form is supplied in the water culture liquid.
In a preferred embodiment, described method also comprises: the wheat seeding that screening is obtained carries out field experiment, establishes normally to handle and hang down nitrogen and handle for nitrogen, and ripe back is measured barley is carried out anti-low nitrogen evaluation and evaluation.
In a preferred embodiment, described nitrogen physiology availability=overground part dry matter/overground part nitrogen accumulation amount * 100%.
Description of drawings
Fig. 1 shows each relativity value of barley, wherein, and 1: relative nitrogen physiology availability; 2: relative individual plant amount of nitrogen sucking; 3: top dry weight relatively; 4: relative height of seedling; 5: relative tiller number; 6: relative grain yield.
Fig. 2 shows the coefficient of variation of each relativity of barley, wherein, and 1: relative nitrogen physiology availability; 2: relative individual plant amount of nitrogen sucking; 3: top dry weight relatively; 4: relative height of seedling; 5: relative tiller number; 6: relative grain yield.
Embodiment
The application provides anti-low nitrogen screening of a kind of barley and the method for identifying, this method comprises:
1) use water culture liquid to cultivate the barley wheat seeding, if low nitrogen and normal for 2 processing of nitrogen, about 4-5 receives seedling after week, to seedling stage biology and relevant physiological index investigate, described physical signs comprises nitrogen physiology availability, individual plant amount of nitrogen sucking, overground part dry weight, plant height and tiller number, carries out anti-low nitrogen evaluation in seedling stage and screening;
2) carry out field experiment with identical kind at Mai Zuoqu, establish normal nitrogen fertilizer application and not 2 processing of nitrogen fertilizer application, ripe back is surveyed to produce barley is carried out anti-low nitrogen evaluation and evaluation;
3) set up the correlation of seedling stage and the anti-low nitrogen of maturing stage barley, establish screening index in effective seedling stage, thereby the anti-low nitrogen of barley is carried out scale and screening fast and effectively in seedling stage.
Water culture test is carried out in the greenhouse, laboratory, and the collocation method of water culture liquid is with reference to international paddy rice institute nutrient solution prescription, and can change slightly.
In one embodiment, the prescription of water culture liquid can be as shown in table 1 below (unit: mg/L):
Table 1
Macroelement:
NH
4NO
3 114.3
NaH
2PO
4·2H
2O 50.4
K
2SO
4 89.3
CaCl
2 110.8
MgSO
4·7H
2O 405.0
Trace element:
MnCl
2·4H
2O 1.875
(NH
4)
6Mo
7O
24·2H
2O 0.093
H
3BO
3 1.168
ZnSO
4·7H
2O 0.044
CuSO
4·5H
2O 0.039
Molysite:
FeSO
4·7H
2O 0.028
Na
2EDTA 0.037
Agents useful for same is chemical reagent commonly used, just can buy in general chemical reagents corporation, and culture fluid pH is usually in the 5.5-6.0 scope.Preferably, the pH of this water culture liquid can be in the scope of 5.6-5.8.
It will be appreciated by those skilled in the art that, can make suitable change to the concentration shown in the last table, for example have about 5% (for example about 3%, about 2%, about 1%, or lower mobility scale) or lower change, prepare the medium that obtains thus and still have required biological function, still can be used to implement the present invention.For example, with MgSO
47H
2O is an example, can contain the MgSO of about 384.8-425.3mg in every liter of culture fluid
47H
2O (about 5% amplitude of fluctuation).The concentration of other composition also can so change.In addition, but the composition in this water culture liquid also replaced by function of use and the same or analogous composition of character.
Implementing when of the present invention, can first disinfection seed, for example sterilized about 30 minutes with 1%NaClO, with flushing with clean water clean after, 30 ± 2 ℃ of seed soaking 4-5 hour, 25 ± 2 ℃ of vernalization were spent the night, and are seeded in the vermiculite of autoclave sterilization after showing money or valuables one carries unintentionally.Can be wholeheartedly the time at two leaves, select the seedling of neat and consistent, go to be fixed on the cystosepiment of punching with sponge strip behind the endosperm, put into the Turnover Box that fills clear water (40cm * 27cm * 10cm) for example then, recovery two is changed nutrient solution as previously described to receipts after three days, is evenly distributed about 30 holes (7 * 5) on every block of cystosepiment.
In one embodiment, establish 3 kinds, low nitrogen (0.4mmol/L) and normal for 2 processing of nitrogen (2mmol/L) is established in each kind 5 strain.
The nitrogen concentration scope of low nitrogen can be 0.1-0.8mmol/L, for example can be 0.2-0.6mmol/L, more preferably 0.4-0.5mmol/L.
Normal nitrogen concentration scope for nitrogen can be 1-3mmol/L, for example can be 1.5-2.5mmol/L, more preferably 1.8-2.2mmol/L.
In one embodiment, the nitrogen concentration of low nitrogen is about 0.4mmol/L, and normally the nitrogen concentration for nitrogen is about 2mmol/L.
Nitrogen can be with NH
4NO
3The form supply also can other inorganic nitrogen-sourced form provide.
Can change one time of nutrition liquid weekly, keep the skin wet every day, and transferring pH is about 5.5 ± 0.2.Sick, insect pest is managed according to a conventional method, and about 5 weeks back receipts seedling investigation index of correlation is as nitrogen physiology availability, individual plant amount of nitrogen sucking, overground part dry weight, height of seedling and tiller number.Like this barley is carried out preliminary anti-low nitrogen evaluation seedling stage.Relevant physical signs can followingly be calculated:
Nitrogen content=overground part nitrogen accumulation amount/overground part dry matter * 100%
Nitrogen physiology availability=overground part dry matter/overground part nitrogen accumulation amount * 100%
Individual plant amount of nitrogen sucking=nitrogen content * average individual plant overground part dry weight
In the specific implementation, in the water culture test, the seedling of results is rinsed well with running water earlier, uses deionized water rinsing again, measure height of seedling, number is tillered, and gets the overground part seedling then, completes under 105 ± 5 ℃ respectively about 30 minutes, dry to constant weight under 70 ± 3 ℃, measure the overground part dry matter, levigate mistake 0.25 mm sieve, Kjeldahl is measured full nitrogen.By calculating the overground part nitrogen accumulation amount that just can draw.Average individual plant overground part dry weight is measured overground part dry gross weight/be used to the detect wheat seeding sum of overground part dry weight.
Field trial is carried out on the farm of proving ground.In an embodiment, the test location is paddy and wheat crop rotation ground, and soil property is a loam, and about 20 ± 3 cm thicks of topsoil contain organic 34.5 ± 3g/kg, full nitrogen 2.42 ± 0.3g/kg, quick-acting nitrogen 37.35 ± 3mg/kg.Split plot design is adopted in test, the primary area is that fertilizer is handled, if normal nitrogen fertilizer application and not 2 processing of nitrogen fertilizer application, secondary area can be divided the sub-district of respective numbers according to the kind of actual detected, each sub-district area is about 7.5 square metres, repeat (being that each is handled each kind 3 repetition are all arranged) for 3 times with the difference between the elimination diverse location soil.In one embodiment, secondary area is 3 kinds.After the maturation, the results barley grain is surveyed and is produced.Can carry out true and reliable evaluation and evaluation to the anti-low nitrogen of barley like this.
In one embodiment, the application has obtained three kinds of barley varieties to the nitrogen differential responses, and wherein the anti-low nitrogen of BI-04 is best, and BI-45 is the most responsive, and BI-35 falls between.
The character value that barley seedling stage, low nitrogen was coerced under the confession nitrogen of character value down/normally is barley anti-low nitrogen proterties relative value in seedling stage, grain yield under grain yield under the barley maturing stage do not applied fertilizer/normal fertilising is the anti-low nitrogen relative value of barley, this value is high more, and anti-low nitrogen is also strong more.
By the correlation analysis between anti-low nitrogen proterties of seedling stage anti-low nitrogen proterties and maturing stage, discovery coerces tiller number and grain yield to be extremely significantly positive correlation at low nitrogen, nitrogen physiology availability, relative height of seedling and grain yield all are extremely significantly positive correlation respectively relatively, consider that the relative height of seedling coefficient of variation is especially little, therefore can think and coerce down the relative nitrogen physiology of tiller number availability as the barley important indicator that seedling stage, anti-low nitrogen screened with seedling stage with low nitrogen, and seedling stage relative height of seedling as the assisting sifting index.Thereby for barley carries out anti-low nitrogen evaluation on a large scale fast and effectively and screens service seedling stage.
Through further experimental data that comparative studies obtains discovery, the material of anti-low nitrogen can be thought in barley nitrogen in seedling stage physiology availability relative value 〉=1.8, tiller number relative value 〉=0.8.
Therefore, the method that the application relates to a kind of anti-low nitrogen barley screening and identifies, this method comprises:
Water culture barley wheat seeding is established low nitrogen and normally for 2 processing of nitrogen, about 4-5 receives seedling after week, measures nitrogen physiology availability and tiller number;
Wherein, the wheat seeding of nitrogen physiology availability relative value 〉=1.8, tiller number relative value 〉=0.8 is elected anti-low nitrogen barley as.
In a preferred embodiment, said method also comprises: the wheat seeding that screening is obtained carries out field experiment, establishes normally to handle and hang down nitrogen and handle for nitrogen, and ripe back is measured barley is carried out anti-low nitrogen evaluation and evaluation.
The application's technical scheme is easy to implement, both different with the anti-low nitrogen of only studying seedling stage or maturing stage in the past, also with in the past only in the laboratory or to do anti-low nitrogen research different in the field, but test and the field yield trials by the water culture in seedling stage simultaneously, comparative analysis two kinds of variation situations for barley anti-low nitrogen correlated traits in seedling stage under the nitrogen conditions and maturing stage grain yield, and the correlative relationship of searching anti-low nitrogen correlated traits of seeding stage and maturing stage yield traits, thereby can carry out anti-fast and effectively low nitrogen screening to barley in seedling stage, courageously eliminate the kind of anti-low nitrogen difference in seedling stage, the good kind of anti-low nitrogen in seedling stage is carried out yield trials carrying out the field, thereby obtain the real good kind of anti-low nitrogen, real result is credible.
Applicant's experiment shows, can obtain anti-low nitrogen screening of effective barley and identification of indicator by this method, and accurately distinguished anti-low nitrogen type, nitrogen responsive type and three kinds of barley variety types of osculant, also for seedling stage extensive anti-low nitrogen screening varieties lay a good foundation.
Hereinafter will be that present invention is described for example with the barley.Should be understood that under the situation that does not depart from spirit and scope of the invention and can make various modifications and changes the present invention.Following examples only are illustrative, and scope of the present invention is limited by the application's claim.In addition, should be understood that the type of above-mentioned each reagent can be made certain modification, as long as the supply of satisfying coherent element is to realize goal of the invention of the present invention.In addition, the resulting technical scheme of the combination disclosed various preferable range of the application is also within the application's protection domain.
Embodiment
Embodiment 1: utilize evaluation and the screening of solution trial to barley anti-low nitrogen in seedling stage
The water culture test is carried out in the greenhouse, laboratory, and the nutrient solution collocation method is with reference to international paddy rice institute's nutrient solution prescription (slightly changing).Seed was sterilized 30 minutes with 1%NaClO, clean with flushing with clean water, soaked seed 5 hours for 30 ℃, 25 ℃ of vernalization are spent the night, be seeded into after showing money or valuables one carries unintentionally in the vermiculite of autoclave sterilization, two leaves are wholeheartedly the time, select the seedling of neat and consistent, go to be fixed on the cystosepiment of punching with sponge strip behind the endosperm, put into the Turnover Box that fills clear water (40cm * 27cm * 10cm) then, recover to change two days later nutrient solution, be evenly distributed 30 holes (7 * 5) on every block of cystosepiment, establish 3 kinds, each kind 5 strain, if low nitrogen (0.4mmol/L) and normal for 2 processing of nitrogen (2mmol/L), with NH
4NO
3The form supply, one time of nutrition liquid is changed in 3 repetitions weekly, keep the skin wet every day, and transferring pH is about 5.5, and sick, insect pest is managed according to a conventional method, 5 weeks back receipts seedling investigation index of correlation is as nitrogen physiology availability, individual plant amount of nitrogen sucking, overground part dry weight, height of seedling and tiller number.Like this barley is carried out preliminary anti-low nitrogen evaluation seedling stage.Experimental result sees the following form 2 and 3.
Nitrogen content=overground part nitrogen accumulation amount/overground part dry matter * 100%
Nitrogen physiology availability=overground part dry matter/overground part nitrogen accumulation amount * 100%
Individual plant amount of nitrogen sucking=nitrogen content * average individual plant overground part dry weight
Overground part dry matter and overground part nitrogen accumulation amount are measured as mentioned before.
Table 2: the genotypic difference that normally supplies barley correlated traits under the nitrogen level
Table 3: low nitrogen is coerced down the genotypic difference of barley correlated traits
Embodiment 2: utilize evaluation and the evaluation of field experiment to anti-low nitrogen of barley maturing stage
Field trial is carried out on the farm of proving ground, and this test location is paddy and wheat crop rotation ground, and soil property is a loam, and top layer 20 cm thick soil contain organic 34.5g/kg, full nitrogen 2.42g/kg, quick-acting nitrogen 37.35mg/kg.Split plot design is adopted in test, and the primary area is that fertilizer is handled, and establishes normal nitrogen fertilizer application and not 2 processing of nitrogen fertilizer application, and secondary area is 3 kinds, and each sub-district area is about 7.5 square metres, 3 repetitions.After the maturation, the results barley grain is surveyed and is produced.Can carry out true and reliable evaluation and evaluation to the anti-low nitrogen of barley like this.Employed barley and embodiment test employed barley and belong to same breed 1 seedling stages.
Experiment has also obtained three kinds of barley varieties to the nitrogen differential responses simultaneously, and wherein the anti-low nitrogen of BI-04 is best, and BI-45 is the most responsive, and BI-35 falls between.Experimental result sees the following form 4.
Table 4: barley grain yield traits genotypic difference under two kinds of Fertilization Level
Embodiment 3: to the analysis of variance of barley seedling stage and anti-low nitrogen proterties relative value of maturing stage
The character value that barley seedling stage, low nitrogen was coerced under the confession nitrogen of character value down/normally is barley anti-low nitrogen proterties relative value in seedling stage, grain yield under grain yield under the barley maturing stage do not applied fertilizer/normal fertilising is the anti-low nitrogen relative value of barley, this value is high more, and anti-low nitrogen is also strong more.Experimental result is seen Fig. 1 and 2.
Embodiment 4: set up the correlation of seedling stage and the anti-low nitrogen of maturing stage barley, establish screening index in effective seedling stage
By the correlation analysis between anti-low nitrogen proterties of seedling stage anti-low nitrogen proterties and maturing stage, discovery coerces tiller number and grain yield to be extremely significantly positive correlation at low nitrogen, nitrogen physiology availability, relative height of seedling and grain yield all are extremely significantly positive correlation respectively relatively, consider that the relative height of seedling coefficient of variation is especially little, therefore can think and coerce down the relative nitrogen physiology of tiller number availability as the barley important indicator that seedling stage, anti-low nitrogen screened with seedling stage with low nitrogen, and seedling stage relative height of seedling as the assisting sifting index.Thereby for barley carries out anti-low nitrogen evaluation on a large scale fast and effectively and screens service seedling stage.The experimental result 5-7 that sees the following form.
Table 5: normally supply the correlation analysis between each proterties of barley under the nitrogen
Table 6: low nitrogen is coerced down the correlation analysis between each proterties of barley
Table 7: each correlated traits relative value correlation analysis of barley
*p≤0.05；
**p≤0.01。
Claims (10)
1. anti-low nitrogen screening of a barley and the method identified, this method comprises:
1) use water culture liquid to cultivate the barley wheat seeding, if low nitrogen and normal for 2 processing of nitrogen, about 4-5 receives seedling after week, to seedling stage biology and relevant physiological index investigate, described physical signs comprises nitrogen physiology availability, individual plant amount of nitrogen sucking, overground part dry weight, plant height and tiller number, carries out anti-low nitrogen evaluation in seedling stage and screening;
2) carry out field experiment with identical kind at Mai Zuoqu, establish normal nitrogen fertilizer application and not 2 processing of nitrogen fertilizer application, ripe back is surveyed to produce barley is carried out anti-low nitrogen evaluation and evaluation;
3) set up the correlation of seedling stage and the anti-low nitrogen of maturing stage barley, establish the screening index in seedling stage, thereby the anti-low nitrogen of barley is screened in seedling stage.
2. the method for claim 1 is characterized in that, the nitrogen concentration scope of described low nitrogen is 0.1-0.8mmol/L, and normally the nitrogen concentration scope for nitrogen is 1-3mmol/L.
3. method as claimed in claim 1 or 2 is characterized in that, the pH of described water culture liquid is 5.5-6.0, and described nitrogenous source is with NH
4NO
3Form is supplied in the water culture liquid.
4. as each described method among the claim 1-3, it is characterized in that described seedling stage, screening index was a nitrogen physiology availability and tiller number in seedling stage.
5. method as claimed in claim 4, it is characterized in that, screening obtains the wheat seeding of nitrogen physiology availability relative value in seedling stage 〉=1.8, tiller number relative value 〉=0.8, it is anti-low nitrogen barley wheat seeding, wherein, described seedling stage, nitrogen physiology availability relative value was coerced character value under the confession nitrogen of character value down/normally for low nitrogen.
6. an anti-low nitrogen barley screening and the method identified, this method comprises:
Use water culture liquid is cultivated the barley wheat seeding, establishes low nitrogen and normally supplies 2 processing of nitrogen, and 4-5 receives seedling after week, measures nitrogen physiology availability and tiller number;
Wherein, the wheat seeding of nitrogen physiology availability relative value 〉=1.8, tiller number relative value 〉=0.8 is elected anti-low nitrogen barley as,
Wherein, described seedling stage, nitrogen physiology availability relative value was coerced character value under the confession nitrogen of character value down/normally for low nitrogen.
7. method as claimed in claim 6 is characterized in that, the nitrogen concentration scope of described low nitrogen is 0.1-0.8mmol/L, and normally the nitrogen concentration scope for nitrogen is 1-3mmol/L.
8. as claim 6 or 7 described methods, it is characterized in that the pH of described water culture liquid is 5.5-6.0, described nitrogenous source is with NH
4NO
3Form is supplied in the water culture liquid.
9. as each described method among the claim 6-8, it is characterized in that described method also comprises: the wheat seeding that screening is obtained carries out field experiment, establishes normally to handle and hang down nitrogen and handle for nitrogen, and ripe back is measured barley is carried out anti-low nitrogen evaluation and evaluation.
10. the described method of each claim as described above is characterized in that described nitrogen physiology availability=overground part dry matter/overground part nitrogen accumulation amount * 100%.
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| CN102807412A (en) * | 2012-09-14 | 2012-12-05 | 扬州大学 | Barley full-growth-period nutrient solution and barley cultivation method |
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| CN107290487A (en) * | 2017-07-13 | 2017-10-24 | 山东农业大学 | A kind of nitrogen efficiently utilizes screening technique and its application of type apple resource |
| CN108184633A (en) * | 2018-01-10 | 2018-06-22 | 上海市农业科学院 | A kind of screening technique of resistance to low nitrogen barley |
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| CN112273219A (en) * | 2020-10-10 | 2021-01-29 | 上海市农业科学院 | A method for binding F by hybridization1Method for rapidly obtaining stable homozygous nitrogen high-efficiency material by culturing microspore |
| CN114190247A (en) * | 2021-12-13 | 2022-03-18 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | Low-nitrogen-resistant field identification method for garlic |
| CN115508509A (en) * | 2022-09-16 | 2022-12-23 | 吉林省农业科学院 | Evaluation method for low-nitrogen resistance of soybean in seedling stage |
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