CN114945681A - 用于连续核酸检测的方法 - Google Patents

用于连续核酸检测的方法 Download PDF

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Publication number
CN114945681A
CN114945681A CN202080092863.XA CN202080092863A CN114945681A CN 114945681 A CN114945681 A CN 114945681A CN 202080092863 A CN202080092863 A CN 202080092863A CN 114945681 A CN114945681 A CN 114945681A
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probes
subset
label
probe
amplicons
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Pending
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CN202080092863.XA
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English (en)
Chinese (zh)
Inventor
L.王
H.卢
B.张
H.宗
X-J.马
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Advanced Cell Diagnostics Inc
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Advanced Cell Diagnostics Inc
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Publication of CN114945681A publication Critical patent/CN114945681A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/119—Reactions demanding special reaction conditions pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/40—Detection characterised by signal amplification of label

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202080092863.XA 2019-11-20 2020-11-20 用于连续核酸检测的方法 Pending CN114945681A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962938138P 2019-11-20 2019-11-20
US62/938,138 2019-11-20
PCT/US2020/061467 WO2021102237A1 (en) 2019-11-20 2020-11-20 Methods for sequential detection of nucleic acids

Publications (1)

Publication Number Publication Date
CN114945681A true CN114945681A (zh) 2022-08-26

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202080092863.XA Pending CN114945681A (zh) 2019-11-20 2020-11-20 用于连续核酸检测的方法

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Country Link
US (1) US20230002815A1 (https=)
EP (1) EP4061958A4 (https=)
JP (1) JP7837866B2 (https=)
KR (1) KR20220117235A (https=)
CN (1) CN114945681A (https=)
AU (1) AU2020386050A1 (https=)
CA (1) CA3162467A1 (https=)
WO (1) WO2021102237A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114555825A (zh) * 2019-02-15 2022-05-27 领先细胞医疗诊断有限公司 用于通过原位杂交进行核酸的多路复用检测的方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024124041A1 (en) * 2022-12-07 2024-06-13 Advanced Cell Diagnostics, Inc. Multiplexed detection of nucleic acid targets

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070015188A1 (en) * 2005-06-20 2007-01-18 Panomics, Inc. Multiplex detection of nucleic acids
US20190144932A1 (en) * 2016-05-10 2019-05-16 Arizona Board Of Regents On Behalf Of Arizona State University Consecutive hybridization for multiplexed analysis of biological samples

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011038403A1 (en) * 2009-09-28 2011-03-31 Yuling Luo Methods of detecting nucleic acid sequences with high specificity
US11352660B2 (en) * 2016-10-04 2022-06-07 Howard Hughes Medical Institute Materials and methods for serial multiplexed detection of RNA in cells and tissues
EP4339956A3 (en) * 2016-11-08 2024-06-19 President and Fellows of Harvard College Multiplexed imaging using merfish, expansion microscopy, and related technologies
SG11202108618VA (en) * 2019-02-15 2021-09-29 Advanced Cell Diagnostics Inc Methods for multiplex detection of nucleic acids by in situ hybridization

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070015188A1 (en) * 2005-06-20 2007-01-18 Panomics, Inc. Multiplex detection of nucleic acids
US20190144932A1 (en) * 2016-05-10 2019-05-16 Arizona Board Of Regents On Behalf Of Arizona State University Consecutive hybridization for multiplexed analysis of biological samples

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
"Safety Data Sheet - Acetic Acid 28%", Retrieved from the Internet <URL:https://www.geneseo.edu/sites/default/files/2022-10/Acetic%20Acid%20conc.pdf> *
I.M.SHAPIRO ET AL.: "Acetic acid treatment denatures DNA while preserving chromosomal morphology during the in situ hybridization procedure", 《EXPERIMENTAL CELL RESEARCH》, vol. 115, no. 2, 30 September 1978 (1978-09-30), pages 411 - 414, XP024791147, DOI: 10.1016/0014-4827(78)90296-3 *
YASUHIKO KITAYAMA ET AL.: "Repeated fluorescence in situ hybridization by a microwave-enhanced protocol", 《PATHOLOGY INTERNATIONAL》, vol. 56, no. 9, 30 September 2006 (2006-09-30), pages 490 - 493, XP055830408, DOI: 10.1111/j.1440-1827.2006.01996.x *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114555825A (zh) * 2019-02-15 2022-05-27 领先细胞医疗诊断有限公司 用于通过原位杂交进行核酸的多路复用检测的方法
US12571028B2 (en) 2019-02-15 2026-03-10 Advanced Cell Diagnostics, Inc. Methods for multiplex detection of nucleic acids by in situ hybridization

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Publication number Publication date
AU2020386050A1 (en) 2022-06-23
JP7837866B2 (ja) 2026-03-31
KR20220117235A (ko) 2022-08-23
JP2023502663A (ja) 2023-01-25
WO2021102237A1 (en) 2021-05-27
EP4061958A1 (en) 2022-09-28
CA3162467A1 (en) 2021-05-27
EP4061958A4 (en) 2024-04-03
US20230002815A1 (en) 2023-01-05

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