AU2020386050A1 - Methods for sequential detection of nucleic acids - Google Patents

Methods for sequential detection of nucleic acids Download PDF

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Publication number
AU2020386050A1
AU2020386050A1 AU2020386050A AU2020386050A AU2020386050A1 AU 2020386050 A1 AU2020386050 A1 AU 2020386050A1 AU 2020386050 A AU2020386050 A AU 2020386050A AU 2020386050 A AU2020386050 A AU 2020386050A AU 2020386050 A1 AU2020386050 A1 AU 2020386050A1
Authority
AU
Australia
Prior art keywords
amplifiers
probes
label
label probes
subsets
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
AU2020386050A
Other languages
English (en)
Inventor
Han Lu
Xiao-Jun Ma
Li Wang
Li-Chong Wang
Bingqing ZHANG
Hailing ZONG
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Advanced Cell Diagnostics Inc
Original Assignee
Advanced Cell Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Cell Diagnostics Inc filed Critical Advanced Cell Diagnostics Inc
Publication of AU2020386050A1 publication Critical patent/AU2020386050A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/119—Reactions demanding special reaction conditions pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/40—Detection characterised by signal amplification of label

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2020386050A 2019-11-20 2020-11-20 Methods for sequential detection of nucleic acids Pending AU2020386050A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962938138P 2019-11-20 2019-11-20
US62/938,138 2019-11-20
PCT/US2020/061467 WO2021102237A1 (en) 2019-11-20 2020-11-20 Methods for sequential detection of nucleic acids

Publications (1)

Publication Number Publication Date
AU2020386050A1 true AU2020386050A1 (en) 2022-06-23

Family

ID=75981712

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2020386050A Pending AU2020386050A1 (en) 2019-11-20 2020-11-20 Methods for sequential detection of nucleic acids

Country Status (8)

Country Link
US (1) US20230002815A1 (https=)
EP (1) EP4061958A4 (https=)
JP (1) JP7837866B2 (https=)
KR (1) KR20220117235A (https=)
CN (1) CN114945681A (https=)
AU (1) AU2020386050A1 (https=)
CA (1) CA3162467A1 (https=)
WO (1) WO2021102237A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11202108618VA (en) * 2019-02-15 2021-09-29 Advanced Cell Diagnostics Inc Methods for multiplex detection of nucleic acids by in situ hybridization
WO2024124041A1 (en) * 2022-12-07 2024-06-13 Advanced Cell Diagnostics, Inc. Multiplexed detection of nucleic acid targets

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2500439T4 (da) * 2005-06-20 2017-11-13 Advanced Cell Diagnostics Inc Kits og produkter til detektering af nukleinsyrer i individuelle celler og til identifikation af sjældne celler fra store heterogene cellepopulationer
WO2011038403A1 (en) * 2009-09-28 2011-03-31 Yuling Luo Methods of detecting nucleic acid sequences with high specificity
US11512344B2 (en) * 2016-05-10 2022-11-29 Arizona Board Of Regents On Behalf Of Arizona State University Consecutive hybridization for multiplexed analysis of biological samples
US11352660B2 (en) * 2016-10-04 2022-06-07 Howard Hughes Medical Institute Materials and methods for serial multiplexed detection of RNA in cells and tissues
EP4339956A3 (en) * 2016-11-08 2024-06-19 President and Fellows of Harvard College Multiplexed imaging using merfish, expansion microscopy, and related technologies
SG11202108618VA (en) * 2019-02-15 2021-09-29 Advanced Cell Diagnostics Inc Methods for multiplex detection of nucleic acids by in situ hybridization

Also Published As

Publication number Publication date
JP7837866B2 (ja) 2026-03-31
KR20220117235A (ko) 2022-08-23
JP2023502663A (ja) 2023-01-25
CN114945681A (zh) 2022-08-26
WO2021102237A1 (en) 2021-05-27
EP4061958A1 (en) 2022-09-28
CA3162467A1 (en) 2021-05-27
EP4061958A4 (en) 2024-04-03
US20230002815A1 (en) 2023-01-05

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