CA3162467A1 - Methods for sequential detection of nucleic acids - Google Patents
Methods for sequential detection of nucleic acidsInfo
- Publication number
- CA3162467A1 CA3162467A1 CA3162467A CA3162467A CA3162467A1 CA 3162467 A1 CA3162467 A1 CA 3162467A1 CA 3162467 A CA3162467 A CA 3162467A CA 3162467 A CA3162467 A CA 3162467A CA 3162467 A1 CA3162467 A1 CA 3162467A1
- Authority
- CA
- Canada
- Prior art keywords
- amplifiers
- probes
- label
- label probes
- subsets
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/119—Reactions demanding special reaction conditions pH
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/40—Detection characterised by signal amplification of label
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962938138P | 2019-11-20 | 2019-11-20 | |
| US62/938,138 | 2019-11-20 | ||
| PCT/US2020/061467 WO2021102237A1 (en) | 2019-11-20 | 2020-11-20 | Methods for sequential detection of nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3162467A1 true CA3162467A1 (en) | 2021-05-27 |
Family
ID=75981712
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3162467A Pending CA3162467A1 (en) | 2019-11-20 | 2020-11-20 | Methods for sequential detection of nucleic acids |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20230002815A1 (https=) |
| EP (1) | EP4061958A4 (https=) |
| JP (1) | JP7837866B2 (https=) |
| KR (1) | KR20220117235A (https=) |
| CN (1) | CN114945681A (https=) |
| AU (1) | AU2020386050A1 (https=) |
| CA (1) | CA3162467A1 (https=) |
| WO (1) | WO2021102237A1 (https=) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG11202108618VA (en) * | 2019-02-15 | 2021-09-29 | Advanced Cell Diagnostics Inc | Methods for multiplex detection of nucleic acids by in situ hybridization |
| WO2024124041A1 (en) * | 2022-12-07 | 2024-06-13 | Advanced Cell Diagnostics, Inc. | Multiplexed detection of nucleic acid targets |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2500439T4 (da) * | 2005-06-20 | 2017-11-13 | Advanced Cell Diagnostics Inc | Kits og produkter til detektering af nukleinsyrer i individuelle celler og til identifikation af sjældne celler fra store heterogene cellepopulationer |
| WO2011038403A1 (en) * | 2009-09-28 | 2011-03-31 | Yuling Luo | Methods of detecting nucleic acid sequences with high specificity |
| US11512344B2 (en) * | 2016-05-10 | 2022-11-29 | Arizona Board Of Regents On Behalf Of Arizona State University | Consecutive hybridization for multiplexed analysis of biological samples |
| US11352660B2 (en) * | 2016-10-04 | 2022-06-07 | Howard Hughes Medical Institute | Materials and methods for serial multiplexed detection of RNA in cells and tissues |
| EP4339956A3 (en) * | 2016-11-08 | 2024-06-19 | President and Fellows of Harvard College | Multiplexed imaging using merfish, expansion microscopy, and related technologies |
| SG11202108618VA (en) * | 2019-02-15 | 2021-09-29 | Advanced Cell Diagnostics Inc | Methods for multiplex detection of nucleic acids by in situ hybridization |
-
2020
- 2020-11-20 US US17/778,612 patent/US20230002815A1/en active Pending
- 2020-11-20 CN CN202080092863.XA patent/CN114945681A/zh active Pending
- 2020-11-20 KR KR1020227020681A patent/KR20220117235A/ko active Pending
- 2020-11-20 EP EP20889509.4A patent/EP4061958A4/en active Pending
- 2020-11-20 WO PCT/US2020/061467 patent/WO2021102237A1/en not_active Ceased
- 2020-11-20 JP JP2022529322A patent/JP7837866B2/ja active Active
- 2020-11-20 CA CA3162467A patent/CA3162467A1/en active Pending
- 2020-11-20 AU AU2020386050A patent/AU2020386050A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| AU2020386050A1 (en) | 2022-06-23 |
| JP7837866B2 (ja) | 2026-03-31 |
| KR20220117235A (ko) | 2022-08-23 |
| JP2023502663A (ja) | 2023-01-25 |
| CN114945681A (zh) | 2022-08-26 |
| WO2021102237A1 (en) | 2021-05-27 |
| EP4061958A1 (en) | 2022-09-28 |
| EP4061958A4 (en) | 2024-04-03 |
| US20230002815A1 (en) | 2023-01-05 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20220919 |
|
| MFA | Maintenance fee for application paid |
Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 4TH ANNIV.) - STANDARD Year of fee payment: 4 |
|
| U00 | Fee paid |
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| U11 | Full renewal or maintenance fee paid |
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|
| D15 | Examination report completed |
Free format text: ST27 STATUS EVENT CODE: A-2-2-D10-D15-D126 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: EXAMINER'S REPORT Effective date: 20250612 |
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| P11 | Amendment of application requested |
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| W00 | Other event occurred |
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| W00 | Other event occurred |
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| P11 | Amendment of application requested |
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| P13 | Application amended |
Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P13-X000 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: APPLICATION AMENDED Effective date: 20251125 |
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| W00 | Other event occurred |
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