CN114929256A - 一种制造合成肉的新方法 - Google Patents

一种制造合成肉的新方法 Download PDF

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CN114929256A
CN114929256A CN202180006833.7A CN202180006833A CN114929256A CN 114929256 A CN114929256 A CN 114929256A CN 202180006833 A CN202180006833 A CN 202180006833A CN 114929256 A CN114929256 A CN 114929256A
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吕陈峰
夏欢
刘文杰
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Abstract

本发明公开了一种通过在可食用生物宿主中重组表达肌肉蛋白来制造合成肉的方法。本发明特别公开了一种制造合成肉的方法，该方法可以克服传统肉类生产实践中大量消耗宝贵水、食物和大片农用面积的问题。此外，本发明公开的方法有助于显著减少动物屠宰。

Description

一种制造合成肉的新方法
本申请要求对2020年1月2日提交的序列号为62/956,398的美国临时专利申请以及2020年2月13日提交的序列号为62/975,913的美国临时专利申请的优先权。当前申请于2021年1月4日提交，因2021年1月2日是周末。
技术领域
本发明总体涉及通过在可食用生物宿主中重组表达肌肉蛋白来制造合成肉的方法。本发明特别涉及一种制造合成肉的方法，该方法可以克服在传统肉类生产实践中大量消耗宝贵的水、食物和大量农用面积的问题。此外，本发明公开的方法有助于显著减少动物屠宰。
背景技术
几千年来，尽管饲养动物作为食物既消耗资源，又不利于环境，人类却一直食用肉类。事实上，畜牧业利用了农业总用地的77％，但只提供了人类食物供应的17％。此外，它用掉了将近三分之一可用于农业的淡水；它向水道排放富含氮和磷的有机物，导致藻类大量繁殖；它使用全球70％的医用抗生素，导致耐药菌株；它经常因不人道和残酷的屠宰行为而受到批评。到2050年，肉类消费预计将增长近50％，现有的畜牧业生产方式无法继续以可持续的方式满足市场需求。此外，食品供应不断受到禽流感和非洲猪瘟等流行病的威胁，这些流行病摧毁了农场的所有动物种群。解决这些问题的一个潜在方法是开发基于植物的肉类替代品。然而，在灵活主义消费者群体广泛采用植物肉方面，仍然存在许多挑战，部分原因是植物肉的味道、香气、质构和营养不如动物肉令人满意。针对上述动物农业影响的另一个潜在解决方案是实验室培育的培养肉，其由通过体外细胞培养生长并收获供人类食用的肌肉组织组成。实验室培育的肉的味道、气味和质构与动物肉相似，但使用该技术面临许多挑战，包括需要昂贵的生长培养基、哺乳动物细胞生长缓慢以及一般的可扩展性问题。在成本和消费者体验方面，以基于植物的肉类和实验室培育的肉类之间仍然存在巨大差距。本发明涉及利用部分或全部与人类或宠物食用的动物肉类的质构和味道非常相似的重组肌肉结构蛋白：肌球蛋白和肌动蛋白来开发新型无动物肉类替代品。我们技术的价值主张在于它相对于现有基于植物的肉类和培养肉类技术的简单性和成本优势。首先，生产Fybraworks食品公司基于真菌的替代蛋白质产品的成本与培养蘑菇菌丝体或酵母的成本相当。合成肉的生产将采用玉米糖或农业残留物的廉价原料和现有成熟的下游工艺进行。接下来，我们的平台将把肌肉蛋白的质构和风味与蘑菇菌丝体蛋白结合起来，而蘑菇菌丝体蛋白本就提供丰富的肉味(鲜味)和质构。
动物肉通常由水、肌肉蛋白、结缔组织、脂肪和血红蛋白(如果是红肉)组成。主要结构蛋白由肌球蛋白和肌动蛋白组成，它们广泛存在于包括酵母在内的生物体中。肌球蛋白属于运动蛋白家族，在肌肉和非肌肉细胞中具有非常多样的细胞功能。骨骼肌细胞中的主要肌球蛋白属于II类肌球蛋白，由两个重链球状头和螺旋结构域加上两对轻链螺旋杆状体结构域组成。
现有的肉类生产设施占据了大片农用面积。此外，目前的肉类生产设施消耗了大量宝贵的水和饲料。此外，目前的技术包括动物屠宰。
因此，需要改进制造合成肉的方法，以克服上述一个或多个问题和/或限制。
发明内容
提供本发明内容是为了以简化的形式介绍一些概念，这些概念将在下面的详细描述中进一步描述。本发明内容并非旨在说明所要求保护的主题的关键特征或基本特征。本发明内容也不旨在限制所要求保护主题的范围。
一般来说，在本发明的一个方面中，公开了一种制造合成肉的方法。因此，该方法可包括酵母或细菌的发酵步骤。
在本发明的另一方面中，所述发酵可以是通过酶的作用在有机基质中产生化学变化的代谢过程。
在本发明的另一方面中，所述酵母可包括毕赤酵母(Pichia pastoris)、酿酒酵母(Saccharomyces cerevisiae)、解脂耶氏酵母菌(Yarrowia lipolytica)等。此外，细菌可包括大肠杆菌(Escherichia coli)、枯草芽孢杆菌(Bacillus subtilis)等。
在本发明的另一个方面中，所述酵母和细菌可以在基本培养基(含有菌落生长所需的最基本营养素)中生长，并通过将糖转化为酒精或将碳水化合物转化为乳酸来获得能量。此外，所述酵母和细菌可以至少繁殖一个数量级以进一步生长。
在本发明的另一方面中，所述方法可包括产生结构肌肉蛋白的步骤。此外，所述结构肌肉蛋白可包括肌球蛋白II和肌动蛋白。通常，肌肉由两条主要的蛋白纤维组成：粗纤维和细纤维。此外，粗纤维可由肌球蛋白组成。
在本发明的另一方面中，所述细纤维可由肌动蛋白组成。此外，结构肌肉蛋白(肌球蛋白和肌动蛋白)存在于包括酵母和细菌在内的活生物体中。在一个实例中，肌球蛋白II可使用发酵过程产生。
在本发明的另一方面中，所述肌球蛋白II可由两条重链、两条必需轻链和两条调节轻链(RLC)组成。
在本发明的另一个方面中，所述结构肌肉蛋白可与动物物种相关，例如但不限于Gallus gallus(红原鸡)、Sus scrofa(野猪)、Bcs Taurus(牛或奶牛)等。
在本发明的另一个方面中，所述方法可包括将结构肌肉蛋白交联成纤维的步骤，以通过使用化学和生物化学过程来产生蛋白纤维。
在本发明的另一个方面中，所述纤维可以是“长链蛋白质，例如在头发、肌肉或鞭毛中发现的蛋白质”。进一步的，所述纤维通常可以捆绑在一起以获得强度和硬度。
在本发明的另一方面中，该方法可包括将蛋白纤维与胶原蛋白、水和脂肪混合以生产合成肉的步骤。此外，所述胶原蛋白为蛋白纤维提供强度和支持。
在本发明的另一方面中，可通过使用一定量的胶原蛋白、水和脂肪来实现蛋白质纤维的混合。进一步的，动物肉通常由水、肌肉蛋白、结缔组织、脂肪和血红蛋白(如果是红肉)组成。
在本发明的另一方面中，所述方法可包括从所述合成肉形成各种肉制品或将所述合成肉与基于植物的蛋白质混合的步骤。
在本发明的另一个方面中，所述肉制品可以包括培根、火腿、热狗、意大利熏火腿、香肠等。此外，基于植物的蛋白质可以是从植物获得的食物蛋白质源。进一步的，基于植物的蛋白质可由鹰嘴豆、花生、杏仁、扁豆等加工而成。
在本发明的另一个方面中，所述肉制品可以配制成猫、狗和其他家畜的干和湿宠物食品。进一步的，所述肉制品也可被家畜和水生物种食用。
在本发明的另一个方面中，所述肌球蛋白和肌动蛋白将在蘑菇中细胞内过表达，例如小型褐菇(Cremini mushroom)、大型顶蘑菇(Portobello mushroom)和纽扣蘑菇(Button mushroom，Agaricus bisporus)、羊肚菌(Morchella esculenta)、香菇(Lentinula edodes)、平菇(Pleurotus ostreatus)、金针菇(Flammulina velutipes)，和牛肝菌(Boletus edulis)。
这些重组蘑菇(孢子)将接种在真菌琼脂平板，如马铃薯葡萄糖琼脂(PDA)或麦芽提取物琼脂(MEA)上以形成菌丝体。这些菌丝体将在谷物和其他富含纤维素物质的培养基上进行固态发酵或深层发酵。菌丝体的生物量将被收获，制成肉制品。通过这种方式，肌球蛋白和肌动蛋白的肉味将与蘑菇的肉质相结合，以模拟肉类的感官效果。
前面的发明内容和下面的详细描述都提供了示例，仅做解释用。因此，上述发明内容和以下详细说明不应被视为是限制性的。此外，除了本文所述的特征或变化之外，还可以提供特征或变化。例如，实施方案可涉及详细描述中描述的各种特征组合和子组合。
附图说明
图1(a)、(b)和(c)表示根据本发明实施方案分别以最小力(左)；中等力(中)；或最大力(右)挤压的无面包屑块凝胶(non-breaded nugget gel)；
图2表示当肌肉蛋白/蘑菇粉达到根据本发明实施方案的比率时获得的均质凝胶基质；
图3表示根据本发明实施方案的表面和底部的烹饪后非面包块部分特征；
图4表示根据本发明实施方案的带干蘑菇的无面包屑块；
图5表示根据本发明实施方案的无面包屑块；
图6(a)和(b)均表示根据本发明实施方案的油炸后的面包屑鸡块；
图7(a)、(b)和(c)所有三个图都代表根据本发明实施方案在烹饪前挤压肉类时的成形性；
图8(a)和(b)表示根据本发明实施方案从鸡肉、酵母、大米和天然调味品成分中提取的肌肉蛋白生产的宠物食品样品(pet treat protype)；
图9表示根据本发明实施方案的使用抗His抗体的SDS-PAGE(左)和蛋白质印迹(右)；
图10表示根据本发明实施方案的使用抗His抗体的SDS-PAGE(左)和蛋白质印迹(右)；
图11表示根据本发明实施方案的肌球蛋白卷曲螺旋(coiled-coil)结构域的蛋白质印迹(抗-His)分析；
图12表示根据本发明实施方案的肌动蛋白的SDS-PAGE(左)和蛋白质印迹(右，抗His)分析。
具体实施方式
作为初步事项，相关领域的普通技术人员将容易理解，本发明具有广泛的实用性和应用性。应当理解，任何实施方案都可以仅包含本发明的一个或多个以上公开的方面，并且还可以仅包含一个或多个以上公开的特征。此外，讨论并确定为“优选”的任何实施方案“被认为是实施本发明实施方案所设想的最佳模式的一部分。在提供完整且可行的发明中，也可以出于额外的说明目的讨论其他实施方案。此外，将讨论许多实施方案，例如适配、变化、修改和等效安排，由本文描述的实施方案隐含地公开，并且属于本发明的范围。
因此，尽管本文针对一个或多个实施方案详细描述了实施方案，但应理解，本公开是本发明的说明和示例，并且仅为提供完整且可行的发明的目的而进行。本文对一个或多个实施方案的详细公开意不在也不应被解释为限制在此公开的专利的任何权利要求中提供的专利保护的范围，该范围将由权利要求及其等价内容定义。专利保护的范围不是通过从任何权利要求解读本文发现的、权利要求本身未明确出现的限制来定义的。
因此，例如，本文描述的各种过程或方法的步骤的任何次序和/或时间顺序是说明性的，而不是限制性的。因此，应当理解，尽管各种过程或方法的步骤可以以次序或时间顺序示出和描述，但任何此类过程或方法的步骤不限于以任何特定次序或顺序执行，除非另有说明。实际上，这些过程或方法中的步骤通常可以以各种不同的次序和顺序执行，同时仍在本发明的范围内。因此，专利保护的范围将由已公开的权利要求而不是本文所述的描述来定义。
此外，需要注意的是，本文中使用的每个术语都是指普通技术人员根据本文中基于该术语的上下文使用理解的该术语的含义。如果普通技术人员根据该术语的上下文使用理解本文中使用的术语的含义与该术语的任何特定词典定义存在任何不同，则应以普通技术人员理解的术语的含义为准。
此外，需要注意的是，如本文所用，“一个(a)”和“一个(an)”通常表示“至少一个”，但不排除多个，除非上下文使用另有规定。当在本文中用于连接项目列表时，或“表示”至少一个项目，但不排除列表中的多个项目。最后，当在本文中用于连接项目列表时，“和(and)”表示“列表中的所有项目”。
以下详细说明参考附图。在可能的情况下，在图纸和以下描述中使用相同的参考号来指代相同或类似的元件。虽然可以描述本发明的许多实施方案，但是可以进行修改、适配和其他实现。例如，可以对附图中所示的元素进行替换、添加或修改，并且可以通过对所公开的方法进行替换、重新排序或添加阶段来修改本文描述的方法。因此，以下详细描述不限制本发明。相反，本发明的适当范围由所附权利要求书限定。本发明包含标题。应当理解，这些标题用作参考，不应被解释为限制标题下披露的主题。
本发明包括许多方面和特征。此外，尽管许多方面和特征与制造合成肉的方法有关，并且在该方法的上下文中描述，但本发明的实施方案不限于仅在该上下文中使用。
本发明描述了一种制造合成肉的方法。此外，该方法可以通过发酵产生主要的肉类结构蛋白，所述蛋白类似于肉类的味道和结构，而无需饲养动物和屠宰。
动物肉通常由水、肌肉蛋白、结缔组织、脂肪和血红蛋白(如果是红肉)组成。主要结构蛋白由肌球蛋白和肌动蛋白组成，它们广泛存在于包括酵母在内的生物体中。肌球蛋白属于运动蛋白家族，在肌肉和非肌肉细胞中具有非常多样的细胞功能。骨骼肌细胞中的主要肌球蛋白属于II类肌球蛋白，由两个重链球状头和螺旋结构域加上两对轻链螺旋杆状结构域组成。
在本发明中，这些关键结构肌肉蛋白，包括肌球蛋白II重链和轻链，以及来自动物物种(如原鸡(Gallus gallus)、野猪(Sus scrofa)和普通牛(Bos taurus))的肌动蛋白，可通过使用酵母(如毕赤酵母、酿酒酵母、以及解脂耶氏酵母菌或大肠杆菌和枯草芽孢杆菌等细菌，它们可以在最基本的培养基中生长，繁殖速度至少比哺乳动物细胞快一个数量级。这些蛋白质也可以在无细胞系统中由酵母(如毕赤酵母、酿酒酵母或解脂耶氏酵母菌)或细菌(如大肠杆菌或枯草芽孢杆菌)或昆虫(如草地贪夜蛾(Spodoptera frugiperda))产生。
或者，如果技术难度太高，无法表达肌球蛋白II，则可以表达其他肌球蛋白，例如肌球蛋白V和VI。然后，这些蛋白质可以通过化学或生化过程交联成纤维。然后，这些蛋白纤维可与胶原蛋白、水和脂肪形成各种肉制品，或与植物蛋白质混合。
因此，所述方法可包括酵母或细菌发酵的步骤。进一步的，发酵可能是一个代谢过程，通过酶的作用在有机基质中产生化学变化。进一步的，酵母可包括毕赤酵母、酿酒酵母、解脂耶氏酵母菌等。进一步的，细菌可包括大肠杆菌、枯草芽孢杆菌等，酵母和细菌可以在基本培养基中生长(含有菌落生长所需的最基本营养素)，并通过将糖转化为酒精或将碳水化合物转化为乳酸来获取能量。进一步的，所述酵母和细菌可以至少繁殖一个数量级以进一步生长。
进一步的，所述方法可包括产生结构肌肉蛋白的步骤。进一步的，结构肌肉蛋白可能包括肌球蛋白II和肌动蛋白。通常，肌肉由两条主要的蛋白纤维组成：粗纤维和细纤维。进一步的，所述粗纤维可能由肌球蛋白组成。进一步的，细纤维可由肌动蛋白组成。进一步的，结构肌肉蛋白(肌球蛋白和肌动蛋白)存在于包括酵母和细菌在内的活生物体中。在一个实例中，肌球蛋白II可使用发酵过程产生。进一步的，肌球蛋白II可能由两条重链、两条必需轻链和两条调节轻链(RLC)组成。进一步的，在一些实施方案中，由于生产肌球蛋白II的技术困难，可以生产肌球蛋白V和肌球蛋白VI，而不是肌球蛋白II。进一步的，结构肌肉蛋白可能与动物物种有关，例如但不限于Gallus gallus(红原鸡)、Sus Scrofa(野猪)、BosTaurus(牛或奶牛)等。
进一步的，所述方法可包括将结构肌肉蛋白交联成纤维的步骤，以通过使用化学和生物化学过程产生蛋白纤维。进一步的，这些纤维可能是“长链蛋白质，如在头发、肌肉或鞭毛中发现的蛋白。进一步的，所述纤维通常可以捆绑在一起以获得强度和硬度。
进一步的，所述方法可包括将蛋白纤维与胶原蛋白、水和脂肪混合以生产合成肉的步骤。进一步的，所述胶原蛋白为蛋白纤维提供强度和支持。进一步的，可通过使用一定量的胶原蛋白、水和脂肪来实现蛋白纤维的混合。进一步的，动物肉通常由水、肌肉蛋白、结缔组织、脂肪和血红蛋白(如果是红肉)组成。
进一步的，所述方法可包括从合成肉形成各种肉制品或将合成肉与基于植物的蛋白质混合的步骤。进一步的，所述肉制品可能包括培根、火腿、热狗、意大利熏火腿、香肠等。进一步的，所述基于植物的蛋白质可能是从植物获得的食物蛋白源。进一步的，基于植物的蛋白质可由鹰嘴豆、花生、杏仁、扁豆等加工而成。
进一步的，ω-3脂肪酸可以添加到合成肉中作为健康红利(bonus)。进一步的，ω-3脂肪酸可能有助于预防和治疗心脏病。进一步的，将携带铁的蛋白质、肌红蛋白添加到合成肉中可产生所需的质构。进一步的，添加肌红蛋白或血红蛋白可能会改变合成肉的颜色，使其看起来更像传统肉。进一步的，合成肉类产品在外观、味道、气味、质构或其他因素上可与传统生产的肉类相似。
进一步的，没有骨头和/或血液可能会使许多传统的肉类制品，如布法罗鸡翅(buffalo wing)，更适合幼儿食用。
对于合成肉，严格的环境控制和组织监测可以从一开始就防止肉类培养物感染，并且在运输给消费者之前可以检测到任何潜在的感染。除了预防和避免疾病，以及不使用抗生素或任何其他化学物质外，合成肉还可以利用许多生物技术进步，包括增加营养强化、单独定制的细胞和分子成分，以及最佳营养状况，所有这些都使它比传统肉类健康得多。
有报道称，功能性重组肌肉肌球蛋白的过表达具有挑战性，因为其体积大，且肌球蛋白折叠所需的细胞机制复杂。迄今为止，功能性肌球蛋白表达仅在小鼠C2C12细胞培养成肌细胞和果蝇(Drosophila melanogaste)中有报道，但从未在低等真核生物或微生物宿主中有报道。肌球蛋白由两条大的重链和两对轻链组装而成，它们排列成一个不对称的分子，两个疏水的球状头部连接在一个长的螺旋杆状亲水尾巴上。肌球蛋白重链已被充分研究，由三个结构域组成：球状头部结构域(重肌球蛋白亚片段-1)和结合肌动蛋白并依靠ATP酶发力的轻链结合颈部结构域(重肌球蛋白亚片段-2)，以及通过卷曲螺旋基序对纤维进行组装至关重要的尾部结构域(轻酶解肌球蛋白)。虽然头部区域对肌肉收缩至关重要，但众所周知，它很难过表达。酶解肌球蛋白(LMM)显示了一种由四个七肽组成的28个残基重复序列的模式，这些七肽被称为skip残基的四个间隔很宽的额外氨基酸打断，这些氨基酸为杆状结构域提供了灵活性。本发明通过表达肌球蛋白的卷曲螺旋结构域来绕过具有挑战性的肌球蛋白表达问题。
肌动蛋白是最丰富的细胞骨架蛋白之一，在几乎所有生命形式中执行多种细胞功能，并在高等真核生物骨骼肌的收缩装置中发挥关键作用。在精确的体内细胞控制下，球状单体肌动蛋白可以聚合成带有倒钩和尖端的极性a-螺旋纤维。它已在几种微生物宿主中重组表达，并在体外生理盐条件下可与生理一价或二价阳离子容易的聚合。
表1示例性肌肉蛋白序列
Figure BDA0003668505180000091
Figure BDA0003668505180000101
其中一种产品是富含肌肉蛋白的酵母抽提物。酵母发酵过程将停止，并进行自发细胞裂解。富含内源性和肌肉蛋白及肽的可溶物将从细胞壁碎片中分离并干燥以供进一步加工。酵母细胞壁的分离不仅能提高蛋白质含量，还能提高产品的消化率。
细胞内或细胞外产生肌肉蛋白的另一个主要宿主家族包括丝状真菌和蘑菇。这些丝状真菌包括可食物种，如Fusarium venenatum、米曲霉(Aspergillus oryzae)、紫红曲霉(Monascus purpureus)、米根霉(Rhizopus oryzae)、间型脉孢菌(Neurosporaintermedia)、木霉属(Trichoderma sp)。这些蘑菇包括小型褐菇(Cremini mushroom)、大型顶蘑菇(Portobello mushroom)和纽扣蘑菇(Button mushroom，Agaricus bisporus)、羊肚菌(Morchella esculenta)、香菇(Lentinula edodes)、平菇(Pleurotus ostreatus)、金针菇(Flammulina velutipes)，和牛肝菌(Boletus edulis)。这些重组蘑菇(孢子)将接种在真菌琼脂平板上，如马铃薯葡萄糖琼脂(PDA)或麦芽提取物琼脂(MEA)以形成菌丝体。这些菌丝体将在谷物和其他富含纤维素物质的培养基上进行固态发酵或深层发酵。菌丝体的生物量将被收获，制成肉制品。通过这种方式，肌球蛋白和肌动蛋白的肉味将与蘑菇的肉质相结合，以模拟肉类的感官效果。
为了改善肉类替代品的质构和味道，可通过化学或生化过程将表达的肌肉蛋白进一步交联成纤维，包括转谷氨酰胺酶和赖氨酸单加氧酶的共表达，以及使用上述酶进行体外交联。这些过程包括以下额外的方法及其组合：
(1)pH调节和预热处理，展开三级结构并影响蛋白质结构域的电荷，以产生排斥力，使凝胶网络保持膨胀状态。
(2)高压过程(HPP)，广泛用于通过变性、聚集或凝胶化来改变蛋白质结构。HPP条件，如压力强度、压力梯度、持续时间、温度等，将针对动物蛋白和菌丝蛋白之间的相互作用进行优化。
(3)通过蛋白质氧化增加二硫键水平，其中蛋白质可以在羟基自由基生成系统中孵育。
(4)精氨酸和赖氨酸含氮侧链上的甲基化，或羟脯氨酸残基修饰的非肌肉蛋白上的羟基化。羰基含量和表面疏水性将发生显著变化，这对凝胶和乳化性能至关重要。
(5)干或湿挤压技术，其是商业上可获得的，并广泛用于豆类(pulse)蛋白。它可以将非凝胶蛋白转化为肉质产品。
(6)亲水胶体，如海藻酸钠(可与钙形成凝胶)、魔芋葡甘聚糖(可与碱形成凝胶)和黄原胶/天然淀粉(可提高乳液稳定性和结合强度)。
这些含有菌丝体或蘑菇的肌肉蛋白随后将与胶原蛋白、水和脂肪混合，和/或与植物蛋白质混合，形成各种肉制品，如热狗、肉干、肉糕、块(nugget)、香肠、培根、意大利腊肠等。
或者，含有酵母或酵母抽提物的肌肉蛋白可以被配制成宠物食品。本发明提供了一种模拟用于宠物食品应用的预备的Fybraworks蛋白质成分(FPI)的组成、功能性和营养价值的新方法。本发明方法涉及用鸡肌原纤维蛋白和/或水解豌豆蛋白模拟蛋白质质构，所述水解豌豆蛋白用L-蛋氨酸和L-组氨酸强化，氨基酸组成与肌肉蛋白相似。Fybraworks配料的蛋白质含量可高达重量基准的50％，可配制成多种不同类别的营养良好的宠物食品，包括处于不同生长阶段的宠物、狗和猫的主食或零食(见表2)。
Fybraworks的蛋白质成分可以由全肌肉蛋白或部分蛋白质和肽组成，它们与全蛋白质具有相同的营养价值，但具有更好的消化率，可以满足低过敏级/兽医处方食品的要求。
Fybraworks的蛋白质成分在整个宠物食品基质中都有独特的风味。提高宠物食品适口性的传统做法是添加动物消化物或适口剂，方法是在干宠物食品表面涂覆涂层，或混入湿宠物食品的汁液部分。这些宠物食品的适口性分布通常不均匀。Fybraworks的蛋白质成分的适口性因肽、氨基酸和糖之间的美拉德反应产生的令人愉悦的肉味以及酵母的鲜味而得到加强。样品的感官测试(图2)证实了一种独特且同质的肉味，这是本地美味宠物食品所没有的。
表2营养全面，消化率和适口性极高的新型不涉及残害动物的(cruelty-free)宠物食品配方的例子
Figure BDA0003668505180000121
*AAFCO：美国饲料管理协会
实施例
以下示例仅是说明本发明的一些方面的一种方式，不应将其解释为限制本发明的范围。
实施例1肌原纤维蛋白提取
从新鲜鸡胸肉中制备肌肉肌原纤维(洗涤肌原纤维)蛋白。在用Stephan混合器切碎之前去除可见的结缔组织和脂肪。将切碎的肉与三份水混合，搅拌，并通过直径为2.5mm的螺旋式进料(screw-fed)过滤器(Bibun Machine，广岛，日本)。然后每次用有机玻璃布将多余的水从肌原纤维提取物中分离出来。除过滤步骤外，所有步骤都再重复两次，但最后一次水洗含有0.5％(w/v)NaCl，以促进脱水。加工结束时，这种富含肌原纤维的洗涤部分的蛋白质含量达到20％w/w，用于所有后续产品开发(PD)。
实施例2由肌肉蛋白和蘑菇粉制成的“鸡”块中肌肉蛋白与蘑菇的比例(样品制备和烹饪程序保持不变)的评估
“鸡”块由肌原纤维蛋白、蘑菇粉、调味料、椰子油、水、磷酸钠制成。分别在3:1(对照)、1.5:1(Tl)、1:5(T2)的条件下测试了不同比例的肌原纤维蛋白(干基)/蘑菇粉。先将磷酸盐与水混合2分钟，然后加入盐、调味料，再混合2分钟，在碗中腌制腌料。将所得糊状物在冰箱中冷却至34°F，并转移至成型设备，以形成一份单一尺寸的块。最后，块要经过预涂粉、打浆和烘烤的步骤。它们将在365°F下预炸30秒，然后在烤箱中完全烤熟，直到产品中心温度达到165°F。在冷藏箱中过夜后进行产品评估。
确定了肌肉蛋白与蘑菇粉的最佳配比(1.5:1)。如图1所示，对照组和T1组均显示出无面包屑部分的弹性纹理。横截面显示出均匀且有弹性的凝胶特征(见图2)。当比例低于1.5:1时，质构变得不那么耐嚼/有弹性，更脆。此外，在T2中观察到大量烹饪损失，如图3所示，其中左侧代表T2(1:5＝肌肉蛋白：蘑菇粉)，右侧代表T1(1.5:1＝肌肉蛋白：蘑菇粉)。显然，烹调后，油溢出到T2样品的表面，并且在每个非面包肉部分的底部发现了更多过度烹调的碎屑。此外，T2样品出现焦糊，因为配方中的蘑菇粉比T1和对照中的蘑菇粉多。
这组实验的结果表明，肌肉蛋白/蘑菇粉的比例对成品的质构很重要，这将指导我们的产品，并为发酵蛋白质含量设定蛋白质含量目标。
实施例3蘑菇粒径和甲基纤维素水平对烹调产品影响的评估(配方、样品制备和烹调程序保持不变)
这个“鸡块”样品的制作程序与前一节所述相同。本实施例中的变量不同：1)在配方中加入甲基纤维素，以进一步改善低蛋白质含量产品的质构(肌肉蛋白/干蘑菇的比例增加至1:9w/w)。在加入其他成分之前，先将其与水高速混合。2)评估了不同粒度的干蘑菇对产品质构的影响。
优选更大粒径(约0.5-1cm)的干蘑菇，因为它产生了更好的质构(见图4)，具有强烈的咬合力和弹性，而较小的粒径(约0.1-0.5cm)产生了脆性凝胶，尽管外观均匀。如图5所示，两种尺寸的蘑菇粉在烹饪时都表现出良好的成型性。
这些发现将有助于改善肉类替代产品的质构和外观，以满足客户的不同需求。
实施例4肌肉蛋白对产品质构和功能影响的评估。
“鸡”块样品的制作程序与前一节中所述的相同。通过包括无肌肉蛋白对照品(用等量的豌豆蛋白代替)评估肌肉蛋白对产品性能的影响。试验配方模拟了一些现成产品，除了干蘑菇试验中使用了大颗粒(见上一节)作为TVP(即基于大豆的)替代品。主要目的是确定肌原纤维蛋白是否会影响产品的外观、质构和面包粘性。
综上所述，煎炸后含有9％肌肉蛋白的产品显示出比对照样品更高的成型性、更强的咬合力和更致密的结构，尽管两个样品在非面包部分显示出相似的凝胶结构，如图6所示。还观察到，该产品的面包粘性略好于对照品。
这些结果表明，与不含肌肉蛋白的对照组相比，含有肌肉肌原纤维蛋白的对照组具有更好的性能。
最后但并非最不重要的一点是，研究发现，由于蘑菇和肌原纤维蛋白之间的协同风味增强了鲜味，至少1/3的盐可以从原配方中去除。这证明了在食品中结合蘑菇和肌原纤维蛋白时，潜在的钠减少作用的额外益处。
实施例5由肌肉蛋白和平菇粉制成的碎肉干
碎肉干(groundjerkey)由肌原纤维蛋白、蘑菇粉、红烧调味料、腌料(cure)、磷酸钠、椰子油、焦糖和水制成。分别以1.5:1、2:1、2.5:1、3:1、3.5:1的比例测试了不同比例的干基肌原纤维蛋白与蘑菇粉。首先，将肌原纤维蛋白与腌料和磷酸钠在食品处理器中以高剪切速度混合1分钟。然后将水和油添加到混合器中，再混合1分钟。接下来，将调味料和蘑菇粉加入混合物中，并在高剪切下混合30秒。最后的面糊在冷却机中固化一整夜后，通过干枪挤压成条状。最后，在165°F的商用脱水机中充分煮熟6小时。产品从脱水机中取出后，将进行真空包装以进行感官评估。
研究发现，肌肉蛋白/蘑菇粉的比例至少为2:1(干基为w/w)对于实现良好的成型性(即无破损)至关重要(见图7)。研究发现，至少2.5:1的比例对于获得良好的肉质干品的嫩度和咀嚼性至关重要(见图7)。如图9所示，在脱水过程中，产品还表现出诸如凝胶和收缩特性的真正的肉特性，这归因于蛋白质变性和聚集。
这些发现将有助于改善肉类替代产品的质构和外观，以满足客户的不同需求。
实施例6将肌肉蛋白配制成宠物食品
将提取的肌原纤维蛋白或用L-蛋氨酸和L-组氨酸强化的水解豌豆蛋白与面包酵母混合，以选定的干重基准比模拟酵母发酵材料。蛋白质物质和酵母的优选干重基准比从5:95到6:94、7:93、8:92、9:91、10:90、11:89、12:88、13:87、14:86、15:85到16:84、17:83、18:82、19:81或20:80之中选择。
本发明提供了使用FPI生产各种宠物食品的新应用方法。FPI首先被彻底水合，以促进离子相互作用、氢键相互作用、范德华相互作用和疏水相互作用。然后加入植物油和甘油，以及调味品和色素成分。然后将混合物充分混合，形成浆。慢慢加入碳水化合物成分，形成均匀的混合物。将材料制成理想形状，在68℃至95℃的温度范围内加热，持续通风1至9小时。宠物食品经过冷却和包装，以尽量减少水分和香气的迁移(见图8)。
优选的植物油选自但不限于菜籽油、葵花籽油、花生油、大豆油、菜籽油、橄榄油、棉籽油、椰子油、玉米油、棕榈油、红花油、芝麻油、杏仁油、腰果油、榛子油、核桃油。
优选的调味品和色素成分选自但不限于盐、各种烟和天然香料、糖蜜、葡萄糖、蔗糖、焦糖色素、磷酸钠、三磷酸钠、海藻酸钠、硫酸钙、磷酸、迷迭香提取物、混合生育酚。
优选的碳水化合物成分选自但不限于大米淀粉、米粉、土豆淀粉、土豆粉、木薯淀粉、木薯粉、豌豆淀粉、豌豆粉、玉米淀粉、玉米粉、燕麦粉、亚麻籽粉、小麦淀粉、小麦粉。
优选的混合过程形式选自泵送、混合、预处理和均质。
加热过程的优选形式是烘焙、脱水、挤压和干馏。
预计用FPI生产宠物食品的方法可以通过连续、分批或两种处理方式的组合。
实施例7肌肉肌球蛋白卷曲螺旋结构域在大肠杆菌中的表达
表3使用的菌株和质粒
Figure BDA0003668505180000151
Figure BDA0003668505180000161
含有编码肌球蛋白卷曲螺旋结构域SEQ ID NO：3的DNA SEQ ID NO：1的表达载体pET-30a(+)，转化到大肠杆菌BL21 StarTM(DE3)感受态细胞中。将单个菌落接种到含有卡那霉素的LB培养基中；培养物在37℃以200rpm培养。当细胞密度在600nm处达到OD＝0.6-0.8时，加入0.5mM IPTG进行诱导。SDS-PAGE和蛋白质印迹用于监测表达(图9)。表达量达到40mg/L，溶解度达到40％。
实施例8肌肉肌动蛋白卷曲螺旋结构域在大肠杆菌中的表达
含有编码肌肉肌动蛋白卷曲螺旋结构域SEQ ID NO：4的DNA序列SEQ ID NO：2的表达载体pET-30a(+)，转化到大肠杆菌BL21 StarTM(DE3)感受态细胞中。
肌肉肌动蛋白卷曲螺旋结构域的表达量达到35mg/L，溶解度小。肌动蛋白缺乏溶解性可能是由于自发聚合，除了43kDa单体，86kDa二聚体和129kDa三聚体也可以从蛋白质印迹中看到(见图10)。
实施例9肌肉肌球蛋白卷曲螺旋结构域在毕赤酵母中的表达
在AOX启动子的控制下将与α因子信号肽和6×His标签(SEQ ID NO：15)融合的肌球蛋白卷曲螺旋结构域序列克隆到整合载体pPICZalphaA，转化到毕赤酵母X-33表达菌株中，在BMGY/BMMY上生长，并通过在28℃以200rpm添加甲醇96h来诱导表达。表达水平较低，但可在细胞颗粒中检测到(见图11)。
实施例10肌肉肌动蛋白卷曲螺旋结构域在毕赤酵母中的表达
在AOX启动子的控制下将与α因子信号肽和6×His标签(SEQ ID NO：14)融合的肌动蛋白编码序列，克隆到整合载体pPICZalphaA，转化到毕赤酵母X-33表达菌株中，在BMGY/BMMY上生长，并通过在28℃和200rpm下添加甲醇诱导表达96h。克隆#6的表达水平最高(见图12)，并且易于自发聚合，这与它在大肠杆菌中的表达类似。
与传统肉类相比，合成肉类有一些潜在的健康、环境、文化和经济考虑。
尽管已经就本发明的优选实施方案解释了本发明，但是应当理解，在不脱离本发明的精神和范围的情况下，可以进行许多其他可能的修改和变化。
序列表
<110> 菲布拉沃克斯食品公司
<120> 一种制造合成肉的新方法
<130> TPC60795
<150> US 62/956398
<151> 2020-01-02
<150> US 62/975913
<151> 2020-02-13
<160> 16
<170> PatentIn version 3.5
<210> 1
<211> 3330
<212> DNA
<213> 原鸡
<400> 1
catatgcacc accaccacca ccacctatta aagagtgcag aatcagaaaa ggagatggcg 60
aacatgaaag aggaattcga gaagaccaaa gaggaactgg cgaaaagcga agcgaagcgt 120
aaagagctgg aggaaaagat ggtggttctg ctgcaggaga agaacgatct gcagctgcaa 180
gttcaagcgg aagcggacag cctggcggat gcggaggaac gttgcgacca actgatcaag 240
accaaaattc agctggaggc gaagatcaaa gaggttaccg aacgtgcgga ggacgaggaa 300
gagattaacg cggaactgac cgcgaagaaa cgtaaactgg aagatgagtg cagcgaactg 360
aagaaagaca tcgacgatct ggagctgacc ctggcgaagg tggaaaagga gaaacacgcg 420
accgagaaca aggtgaaaaa cctgaccgaa gagatggcgg ttctggatga aaccattgcg 480
aaactgacca aggaaaagaa agcgctgcag gaagcgcacc agcaaaccct ggacgatctg 540
caagtggaag aggacaaagt taacaccctg accaaggcga aaaccaagct ggaacagcaa 600
gttgacgatc tggagggtag cctggaacag gagaagaaac tgcgtatgga tctggaacgt 660
gcgaagcgta agctggaggg cgacctgaag ctggcgcacg acagcatcat ggatctggag 720
aacgacaagc agcaactgga tgaaaaactg aagaaaaagg acttcgagat cagccaaatt 780
cagagcaaaa ttgaagacga gcaagcgctg ggtatgcaac tgcagaaaaa gattaaggag 840
ctgcaggcgc gtatcgaaga gctggaagag gaaattgaag cggagcgtac cagccgtgcg 900
aaagcggaaa agcaccgtgc ggatctgagc cgtgaactgg aggaaatcag cgagcgtctg 960
gaggaagcgg gtggcgcgac cgcggcgcag attgagatga acaaaaagcg tgaagcggag 1020
tttcagaaaa tgcgtcgtga cctggaggaa gcgaccctgc agcatgaggc gaccgcggcg 1080
gcgctgcgta aaaagcatgc ggatagcacc gcggaactgg gcgagcagat cgacaacctg 1140
caacgtgtga aacagaagct ggaaaaagag aagagcgaac tgaaaatgga gattgacgat 1200
ctggcgagca acatggaaag cgttagcaaa gcgaaagcga acctggagaa gatgtgccgt 1260
accctggaag atcagctgag cgagatcaaa accaaagagg agcaaaacca gcgtatgatt 1320
aacgacctga acacccaacg tgcgcgtctg cagaccgaaa ccggcgagta cagccgtcag 1380
gcggaggaaa aggatgcgct gatcagccaa ctgagccgtg gtaaacaggg ctttacccag 1440
caaatcgagg aactgaagcg tcacctggag gaagagatta aagcgaagaa cgcgctggcg 1500
catgcgctgc aaagcgcgcg tcacgactgc gaactgctgc gtgagcaata cgaagaggaa 1560
caggaagcga aaggtgaact gcagcgtgcg ctgagcaaag cgaacagcga agtggcgcag 1620
tggcgtacca aatatgagac cgatgcgatc caacgtaccg aggaactgga ggaagcgaaa 1680
aagaaactgg cgcaacgtct gcaggacgcg gaggaacacg tggaagcggt taacgcgaag 1740
tgcgcgagcc tggagaaaac caagcaacgt ctgcagaacg aagttgagga tctgatggtg 1800
gacgttgaac gtagcaacgc ggcgtgcgcg gcgctggata agaaacagaa gaacttcgac 1860
aagatcctgg cggaatggaa acaaaagtac gaggaaaccc agaccgaact ggaggcgagc 1920
cagaaggaaa gccgtagcct gagcaccgag ctgtttaaaa tgaagaacgc gtatgaggaa 1980
agcctggatc acctggaaac cctgaaacgt gagaacaaga acctgcagca agagatcgcg 2040
gacctgaccg aacagattgc ggagggtggc aaggcggtgc acgaactgga gaaagtgaag 2100
aaacacgttg aacaagagaa aagcgagctg caggcgagcc tggaggaagc ggaagcgagc 2160
ctggagcacg aggaaggtaa aatcctgcgt ctgcaactgg aactgaacca gatcaagagc 2220
gagattgacc gtaaaatcgc ggaaaaggat gaggaaatcg accaactgaa gcgtaaccac 2280
ctgcgtattg ttgagagcat gcagagcacc ctggatgcgg aaatccgtag ccgtaacgag 2340
gcgctgcgtc tgaagaaaaa gatggagggt gacctgaacg aaatggagat tcagctgagc 2400
catgcgaacc gtatggcggc ggaagcgcaa aagaacctgc gtaacaccca gggcaccctg 2460
aaagataccc aaatccacct ggacgatgcg ctgcgtaccc aagaagacct gaaagagcag 2520
gtggcgatgg ttgaacgtcg tgcgaacctg ctgcaggcgg aagtggagga actgcgtggc 2580
gcgctggaac agaccgagcg tagccgtaag gtggcggaac aagagctgct ggacgcgacc 2640
gagcgtgttc aactgctgca cacccagaac accagcctga ttaacaccaa aaagaaactg 2700
gagaccgata tcgttcaaat tcagagcgaa atggaggaca ccatccagga agcgcgtaac 2760
gcggaggaga aagcgaagaa agcgattacc gatgcggcga tgatggcgga ggaactgaag 2820
aaagaacagg acaccagcgc gcacctggag cgtatgaaga aaaacatgga tcaaaccgtg 2880
aaggacctgc acgttcgtct ggatgaagcg gagcagctgg cgctgaaggg tggcaagaaa 2940
caactgcaga aactggaggc gcgtgtgcgt gaactggagg gtgaagttga cagcgaacaa 3000
aagcgtagcg cggaggcggt gaaaggcgtt cgtaagtacg aacgtcgtgt gaaagagctg 3060
acctatcagt gcgaggaaga tcgtaagaac atcctgcgtc tgcaggacct ggtggataag 3120
ctgcaaatga aagttaagag ctataaacgt caggcggagg aagcggagga actgagcaac 3180
gttaacctga gcaaattccg taagatccaa cacgaactgg aggaagcgga ggaacgtgcg 3240
gacattgcgg agagccaggt gaacaaactg cgtgttaaga gccgtgaaat tcacggtaag 3300
aaaatcgagg aagaggaata atgaaagctt 3330
<210> 2
<211> 1164
<212> DNA
<213> 原鸡
<400> 2
catatgcacc accaccacca ccactgtgat gaagatgaaa caactgcttt agtttgcgac 60
aacggtagcg gtctggtgaa agcgggtttt gcgggtgatg atgcgccgcg tgcggtgttt 120
ccgagcatcg ttggtcgtcc gcgtcaccag ggcgtgatgg ttggtatggg ccaaaaagac 180
agctacgttg gtgatgaggc gcagagcaag cgtggcatcc tgaccctgaa atatccgatt 240
gaacacggta tcattaccaa ctgggacgat atggagaaga tctggcacca caccttctac 300
aacgaactgc gtgttgcgcc ggaggaacat ccgaccctgc tgaccgaggc gccgctgaac 360
ccgaaggcga accgtgagaa aatgacccag atcatgttcg aaacctttaa cgtgccggcg 420
atgtacgttg cgattcaagc ggtgctgagc ctgtatgcga gcggtcgtac caccggcatc 480
gttctggaca gcggtgatgg cgtgacccac aacgttccga tctacgaagg ttatgcgctg 540
ccgcacgcga ttatgcgtct ggacctggcg ggtcgtgacc tgaccgatta cctgatgaag 600
attctgaccg agcgtggtta tagcttcgtt accaccgcgg agcgtgaaat cgtgcgtgac 660
attaaggaaa aactgtgcta cgttgcgctg gattttgaga acgaaatggc gaccgcggcg 720
agcagcagca gcctggagaa aagctatgaa ctgccggatg gtcaggtgat caccattggc 780
aacgagcgtt ttcgttgccc ggaaaccctg ttccaaccga gctttatcgg tatggagagc 840
gcgggcattc acgaaaccac ctacaacagc atcatgaagt gcgacatcga tattcgtaaa 900
gacctgtacg cgaacaacgt gatgagcggt ggcaccacca tgtatccggg tatcgcggat 960
cgtatgcaga aggagattac cgcgctggcg ccgagcacca tgaagattaa aatcattgct 1020
ccgccggaac gtaaatacag cgtttggatc ggtggcagca ttctggcgag cctgagcacc 1080
ttccagcaaa tgtggatcac caagcaagag tatgatgaag cgggcccgag cattgtgcac 1140
cgtaaatgct tttaatgaaa gctt 1164
<210> 3
<211> 1105
<212> PRT
<213> 原鸡
<400> 3
Met His His His His His His Leu Leu Lys Ser Ala Glu Ser Glu Lys
1 5 10 15
Glu Met Ala Asn Met Lys Glu Glu Phe Glu Lys Thr Lys Glu Glu Leu
20 25 30
Ala Lys Ser Glu Ala Lys Arg Lys Glu Leu Glu Glu Lys Met Val Val
35 40 45
Leu Leu Gln Glu Lys Asn Asp Leu Gln Leu Gln Val Gln Ala Glu Ala
50 55 60
Asp Ser Leu Ala Asp Ala Glu Glu Arg Cys Asp Gln Leu Ile Lys Thr
65 70 75 80
Lys Ile Gln Leu Glu Ala Lys Ile Lys Glu Val Thr Glu Arg Ala Glu
85 90 95
Asp Glu Glu Glu Ile Asn Ala Glu Leu Thr Ala Lys Lys Arg Lys Leu
100 105 110
Glu Asp Glu Cys Ser Glu Leu Lys Lys Asp Ile Asp Asp Leu Glu Leu
115 120 125
Thr Leu Ala Lys Val Glu Lys Glu Lys His Ala Thr Glu Asn Lys Val
130 135 140
Lys Asn Leu Thr Glu Glu Met Ala Val Leu Asp Glu Thr Ile Ala Lys
145 150 155 160
Leu Thr Lys Glu Lys Lys Ala Leu Gln Glu Ala His Gln Gln Thr Leu
165 170 175
Asp Asp Leu Gln Val Glu Glu Asp Lys Val Asn Thr Leu Thr Lys Ala
180 185 190
Lys Thr Lys Leu Glu Gln Gln Val Asp Asp Leu Glu Gly Ser Leu Glu
195 200 205
Gln Glu Lys Lys Leu Arg Met Asp Leu Glu Arg Ala Lys Arg Lys Leu
210 215 220
Glu Gly Asp Leu Lys Leu Ala His Asp Ser Ile Met Asp Leu Glu Asn
225 230 235 240
Asp Lys Gln Gln Leu Asp Glu Lys Leu Lys Lys Lys Asp Phe Glu Ile
245 250 255
Ser Gln Ile Gln Ser Lys Ile Glu Asp Glu Gln Ala Leu Gly Met Gln
260 265 270
Leu Gln Lys Lys Ile Lys Glu Leu Gln Ala Arg Ile Glu Glu Leu Glu
275 280 285
Glu Glu Ile Glu Ala Glu Arg Thr Ser Arg Ala Lys Ala Glu Lys His
290 295 300
Arg Ala Asp Leu Ser Arg Glu Leu Glu Glu Ile Ser Glu Arg Leu Glu
305 310 315 320
Glu Ala Gly Gly Ala Thr Ala Ala Gln Ile Glu Met Asn Lys Lys Arg
325 330 335
Glu Ala Glu Phe Gln Lys Met Arg Arg Asp Leu Glu Glu Ala Thr Leu
340 345 350
Gln His Glu Ala Thr Ala Ala Ala Leu Arg Lys Lys His Ala Asp Ser
355 360 365
Thr Ala Glu Leu Gly Glu Gln Ile Asp Asn Leu Gln Arg Val Lys Gln
370 375 380
Lys Leu Glu Lys Glu Lys Ser Glu Leu Lys Met Glu Ile Asp Asp Leu
385 390 395 400
Ala Ser Asn Met Glu Ser Val Ser Lys Ala Lys Ala Asn Leu Glu Lys
405 410 415
Met Cys Arg Thr Leu Glu Asp Gln Leu Ser Glu Ile Lys Thr Lys Glu
420 425 430
Glu Gln Asn Gln Arg Met Ile Asn Asp Leu Asn Thr Gln Arg Ala Arg
435 440 445
Leu Gln Thr Glu Thr Gly Glu Tyr Ser Arg Gln Ala Glu Glu Lys Asp
450 455 460
Ala Leu Ile Ser Gln Leu Ser Arg Gly Lys Gln Gly Phe Thr Gln Gln
465 470 475 480
Ile Glu Glu Leu Lys Arg His Leu Glu Glu Glu Ile Lys Ala Lys Asn
485 490 495
Ala Leu Ala His Ala Leu Gln Ser Ala Arg His Asp Cys Glu Leu Leu
500 505 510
Arg Glu Gln Tyr Glu Glu Glu Gln Glu Ala Lys Gly Glu Leu Gln Arg
515 520 525
Ala Leu Ser Lys Ala Asn Ser Glu Val Ala Gln Trp Arg Thr Lys Tyr
530 535 540
Glu Thr Asp Ala Ile Gln Arg Thr Glu Glu Leu Glu Glu Ala Lys Lys
545 550 555 560
Lys Leu Ala Gln Arg Leu Gln Asp Ala Glu Glu His Val Glu Ala Val
565 570 575
Asn Ala Lys Cys Ala Ser Leu Glu Lys Thr Lys Gln Arg Leu Gln Asn
580 585 590
Glu Val Glu Asp Leu Met Val Asp Val Glu Arg Ser Asn Ala Ala Cys
595 600 605
Ala Ala Leu Asp Lys Lys Gln Lys Asn Phe Asp Lys Ile Leu Ala Glu
610 615 620
Trp Lys Gln Lys Tyr Glu Glu Thr Gln Thr Glu Leu Glu Ala Ser Gln
625 630 635 640
Lys Glu Ser Arg Ser Leu Ser Thr Glu Leu Phe Lys Met Lys Asn Ala
645 650 655
Tyr Glu Glu Ser Leu Asp His Leu Glu Thr Leu Lys Arg Glu Asn Lys
660 665 670
Asn Leu Gln Gln Glu Ile Ala Asp Leu Thr Glu Gln Ile Ala Glu Gly
675 680 685
Gly Lys Ala Val His Glu Leu Glu Lys Val Lys Lys His Val Glu Gln
690 695 700
Glu Lys Ser Glu Leu Gln Ala Ser Leu Glu Glu Ala Glu Ala Ser Leu
705 710 715 720
Glu His Glu Glu Gly Lys Ile Leu Arg Leu Gln Leu Glu Leu Asn Gln
725 730 735
Ile Lys Ser Glu Ile Asp Arg Lys Ile Ala Glu Lys Asp Glu Glu Ile
740 745 750
Asp Gln Leu Lys Arg Asn His Leu Arg Ile Val Glu Ser Met Gln Ser
755 760 765
Thr Leu Asp Ala Glu Ile Arg Ser Arg Asn Glu Ala Leu Arg Leu Lys
770 775 780
Lys Lys Met Glu Gly Asp Leu Asn Glu Met Glu Ile Gln Leu Ser His
785 790 795 800
Ala Asn Arg Met Ala Ala Glu Ala Gln Lys Asn Leu Arg Asn Thr Gln
805 810 815
Gly Thr Leu Lys Asp Thr Gln Ile His Leu Asp Asp Ala Leu Arg Thr
820 825 830
Gln Glu Asp Leu Lys Glu Gln Val Ala Met Val Glu Arg Arg Ala Asn
835 840 845
Leu Leu Gln Ala Glu Val Glu Glu Leu Arg Gly Ala Leu Glu Gln Thr
850 855 860
Glu Arg Ser Arg Lys Val Ala Glu Gln Glu Leu Leu Asp Ala Thr Glu
865 870 875 880
Arg Val Gln Leu Leu His Thr Gln Asn Thr Ser Leu Ile Asn Thr Lys
885 890 895
Lys Lys Leu Glu Thr Asp Ile Val Gln Ile Gln Ser Glu Met Glu Asp
900 905 910
Thr Ile Gln Glu Ala Arg Asn Ala Glu Glu Lys Ala Lys Lys Ala Ile
915 920 925
Thr Asp Ala Ala Met Met Ala Glu Glu Leu Lys Lys Glu Gln Asp Thr
930 935 940
Ser Ala His Leu Glu Arg Met Lys Lys Asn Met Asp Gln Thr Val Lys
945 950 955 960
Asp Leu His Val Arg Leu Asp Glu Ala Glu Gln Leu Ala Leu Lys Gly
965 970 975
Gly Lys Lys Gln Leu Gln Lys Leu Glu Ala Arg Val Arg Glu Leu Glu
980 985 990
Gly Glu Val Asp Ser Glu Gln Lys Arg Ser Ala Glu Ala Val Lys Gly
995 1000 1005
Val Arg Lys Tyr Glu Arg Arg Val Lys Glu Leu Thr Tyr Gln Cys
1010 1015 1020
Glu Glu Asp Arg Lys Asn Ile Leu Arg Leu Gln Asp Leu Val Asp
1025 1030 1035
Lys Leu Gln Met Lys Val Lys Ser Tyr Lys Arg Gln Ala Glu Glu
1040 1045 1050
Ala Glu Glu Leu Ser Asn Val Asn Leu Ser Lys Phe Arg Lys Ile
1055 1060 1065
Gln His Glu Leu Glu Glu Ala Glu Glu Arg Ala Asp Ile Ala Glu
1070 1075 1080
Ser Gln Val Asn Lys Leu Arg Val Lys Ser Arg Glu Ile His Gly
1085 1090 1095
Lys Lys Ile Glu Glu Glu Glu
1100 1105
<210> 4
<211> 383
<212> PRT
<213> 原鸡
<400> 4
Met His His His His His His Cys Asp Glu Asp Glu Thr Thr Ala Leu
1 5 10 15
Val Cys Asp Asn Gly Ser Gly Leu Val Lys Ala Gly Phe Ala Gly Asp
20 25 30
Asp Ala Pro Arg Ala Val Phe Pro Ser Ile Val Gly Arg Pro Arg His
35 40 45
Gln Gly Val Met Val Gly Met Gly Gln Lys Asp Ser Tyr Val Gly Asp
50 55 60
Glu Ala Gln Ser Lys Arg Gly Ile Leu Thr Leu Lys Tyr Pro Ile Glu
65 70 75 80
His Gly Ile Ile Thr Asn Trp Asp Asp Met Glu Lys Ile Trp His His
85 90 95
Thr Phe Tyr Asn Glu Leu Arg Val Ala Pro Glu Glu His Pro Thr Leu
100 105 110
Leu Thr Glu Ala Pro Leu Asn Pro Lys Ala Asn Arg Glu Lys Met Thr
115 120 125
Gln Ile Met Phe Glu Thr Phe Asn Val Pro Ala Met Tyr Val Ala Ile
130 135 140
Gln Ala Val Leu Ser Leu Tyr Ala Ser Gly Arg Thr Thr Gly Ile Val
145 150 155 160
Leu Asp Ser Gly Asp Gly Val Thr His Asn Val Pro Ile Tyr Glu Gly
165 170 175
Tyr Ala Leu Pro His Ala Ile Met Arg Leu Asp Leu Ala Gly Arg Asp
180 185 190
Leu Thr Asp Tyr Leu Met Lys Ile Leu Thr Glu Arg Gly Tyr Ser Phe
195 200 205
Val Thr Thr Ala Glu Arg Glu Ile Val Arg Asp Ile Lys Glu Lys Leu
210 215 220
Cys Tyr Val Ala Leu Asp Phe Glu Asn Glu Met Ala Thr Ala Ala Ser
225 230 235 240
Ser Ser Ser Leu Glu Lys Ser Tyr Glu Leu Pro Asp Gly Gln Val Ile
245 250 255
Thr Ile Gly Asn Glu Arg Phe Arg Cys Pro Glu Thr Leu Phe Gln Pro
260 265 270
Ser Phe Ile Gly Met Glu Ser Ala Gly Ile His Glu Thr Thr Tyr Asn
275 280 285
Ser Ile Met Lys Cys Asp Ile Asp Ile Arg Lys Asp Leu Tyr Ala Asn
290 295 300
Asn Val Met Ser Gly Gly Thr Thr Met Tyr Pro Gly Ile Ala Asp Arg
305 310 315 320
Met Gln Lys Glu Ile Thr Ala Leu Ala Pro Ser Thr Met Lys Ile Lys
325 330 335
Ile Ile Ala Pro Pro Glu Arg Lys Tyr Ser Val Trp Ile Gly Gly Ser
340 345 350
Ile Leu Ala Ser Leu Ser Thr Phe Gln Gln Met Trp Ile Thr Lys Gln
355 360 365
Glu Tyr Asp Glu Ala Gly Pro Ser Ile Val His Arg Lys Cys Phe
370 375 380
<210> 5
<211> 1938
<212> PRT
<213> 普通牛
<400> 5
Met Ser Ser Asp Gln Glu Met Ala Val Phe Gly Glu Ala Ala Pro Tyr
1 5 10 15
Leu Arg Lys Ser Glu Lys Glu Arg Ile Glu Ala Gln Asn Lys Pro Phe
20 25 30
Asp Ala Lys Thr Ser Val Phe Val Ala Asp Pro Lys Glu Ser Phe Val
35 40 45
Lys Ala Thr Val Gln Ser Arg Glu Gly Gly Lys Val Thr Ala Lys Thr
50 55 60
Glu Ala Gly Ala Thr Val Thr Val Lys Glu Asp Gln Val Phe Pro Met
65 70 75 80
Asn Pro Pro Lys Phe Asp Lys Ile Glu Asp Met Ala Met Met Thr His
85 90 95
Leu His Glu Pro Ala Val Leu Tyr Asn Leu Lys Glu Arg Tyr Ala Ala
100 105 110
Trp Met Ile Tyr Thr Tyr Ser Gly Leu Phe Cys Val Thr Val Asn Pro
115 120 125
Tyr Lys Trp Leu Pro Val Tyr Asn Ala Glu Val Val Thr Ala Tyr Arg
130 135 140
Gly Lys Lys Arg Gln Glu Ala Pro Pro His Ile Phe Ser Ile Ser Asp
145 150 155 160
Asn Ala Tyr Gln Phe Met Leu Thr Asp Arg Glu Asn Gln Ser Ile Leu
165 170 175
Ile Thr Gly Glu Ser Gly Ala Gly Lys Thr Val Asn Thr Lys Arg Val
180 185 190
Ile Gln Tyr Phe Ala Thr Ile Ala Val Thr Gly Glu Lys Lys Lys Glu
195 200 205
Glu Pro Thr Ser Gly Lys Met Gln Gly Thr Leu Glu Asp Gln Ile Ile
210 215 220
Ser Ala Asn Pro Leu Leu Glu Ala Phe Gly Asn Ala Lys Thr Val Arg
225 230 235 240
Asn Asp Asn Ser Ser Arg Phe Gly Lys Phe Ile Arg Ile His Phe Gly
245 250 255
Thr Thr Gly Lys Leu Ala Ser Ala Asp Ile Glu Thr Tyr Leu Leu Glu
260 265 270
Lys Ser Arg Val Thr Phe Gln Leu Lys Ala Glu Arg Ser Tyr His Ile
275 280 285
Phe Tyr Gln Ile Met Ser Asn Lys Lys Pro Glu Leu Ile Glu Met Leu
290 295 300
Leu Ile Thr Thr Asn Pro Tyr Asp Tyr Ala Tyr Val Ser Gln Gly Glu
305 310 315 320
Ile Thr Val Pro Ser Ile Asp Asp Gln Glu Glu Leu Met Ala Thr Asp
325 330 335
Ser Ala Ile Glu Ile Leu Gly Phe Thr Ser Asp Glu Arg Val Ser Ile
340 345 350
Tyr Lys Leu Thr Gly Ala Val Met His Tyr Gly Asn Leu Lys Phe Lys
355 360 365
Gln Lys Gln Arg Glu Glu Gln Ala Glu Pro Asp Gly Thr Glu Val Ala
370 375 380
Asp Lys Ala Ala Tyr Leu Gln Gly Leu Asn Ser Ala Asp Leu Leu Lys
385 390 395 400
Ala Leu Cys Tyr Pro Arg Val Lys Val Gly Asn Glu Phe Val Thr Lys
405 410 415
Gly Gln Thr Val Glu Gln Val Tyr Asn Ala Val Gly Ala Leu Ala Lys
420 425 430
Ala Val Tyr Asp Lys Met Phe Leu Trp Met Val Ala Arg Ile Asn Gln
435 440 445
Gln Leu Asp Thr Lys Gln Pro Arg Gln Tyr Phe Ile Gly Val Leu Asp
450 455 460
Ile Ala Gly Phe Glu Ile Phe Asp Phe Asn Ser Leu Glu Gln Leu Cys
465 470 475 480
Ile Asn Phe Thr Asn Glu Lys Leu Gln Gln Phe Phe Asn His His Met
485 490 495
Phe Val Leu Glu Gln Glu Glu Tyr Lys Lys Glu Gly Ile Glu Trp Glu
500 505 510
Phe Ile Asp Phe Gly Met Asp Leu Ala Ala Cys Ile Glu Leu Ile Glu
515 520 525
Lys Pro Met Gly Ile Phe Ser Ile Leu Glu Glu Glu Cys Met Phe Pro
530 535 540
Lys Ala Thr Asp Met Ser Phe Lys Asn Lys Leu Tyr Glu Gln His Leu
545 550 555 560
Gly Lys Ser Asn Asn Phe Gln Lys Pro Lys Pro Ala Lys Gly Lys Ala
565 570 575
Glu Ala His Phe Ser Leu Ile His Tyr Ala Gly Thr Val Asp Tyr Asn
580 585 590
Ile Thr Gly Trp Leu Asp Lys Asn Lys Asp Pro Leu Asn Glu Thr Val
595 600 605
Val Gly Leu Tyr Gln Lys Ser Ser Val Lys Thr Leu Ala Leu Leu Phe
610 615 620
Ser Gly Pro Ala Ser Gly Glu Ala Glu Gly Gly Pro Lys Lys Gly Gly
625 630 635 640
Lys Lys Lys Gly Ser Ser Phe Gln Thr Val Ser Ala Leu Phe Arg Glu
645 650 655
Asn Leu Asn Lys Leu Met Thr Asn Leu Arg Ser Thr His Pro His Phe
660 665 670
Val Arg Cys Ile Ile Pro Asn Glu Thr Lys Thr Pro Gly Ala Met Glu
675 680 685
His Glu Leu Val Leu His Gln Leu Arg Cys Asn Gly Val Leu Glu Gly
690 695 700
Ile Arg Ile Cys Arg Lys Gly Phe Pro Ser Arg Ile Leu Tyr Ala Asp
705 710 715 720
Phe Lys Gln Arg Tyr Lys Val Leu Asn Ala Ser Ala Ile Pro Glu Gly
725 730 735
Gln Phe Ile Asp Ser Lys Lys Ala Ser Glu Lys Leu Leu Ala Ser Ile
740 745 750
Asp Val Asp His Thr Gln Tyr Lys Phe Gly His Thr Lys Val Phe Phe
755 760 765
Lys Ala Gly Leu Leu Gly Leu Leu Glu Glu Met Arg Asp Glu Lys Leu
770 775 780
Ala Gln Leu Ile Thr Arg Thr Gln Ala Arg Cys Arg Gly Phe Leu Ala
785 790 795 800
Arg Val Glu Tyr Gln Lys Met Val Glu Arg Arg Glu Ser Ile Phe Cys
805 810 815
Ile Gln Tyr Asn Val Arg Ala Phe Met Asn Val Lys His Trp Pro Trp
820 825 830
Met Lys Leu Tyr Phe Lys Ile Lys Pro Leu Leu Lys Ser Ala Glu Thr
835 840 845
Glu Lys Glu Met Ala Asn Met Lys Glu Glu Phe Glu Lys Thr Lys Glu
850 855 860
Glu Leu Ala Lys Ser Glu Ala Lys Arg Lys Glu Leu Glu Glu Lys Met
865 870 875 880
Val Thr Leu Thr Gln Glu Lys Asn Asp Leu Gln Leu Gln Val Gln Ser
885 890 895
Glu Ala Asp Ala Leu Ala Asp Ala Glu Glu Arg Cys Asp Gln Leu Ile
900 905 910
Lys Thr Lys Ile Gln Leu Glu Ala Lys Ile Lys Glu Val Thr Glu Arg
915 920 925
Ala Glu Asp Glu Glu Glu Ile Asn Ala Glu Leu Thr Ala Lys Lys Arg
930 935 940
Lys Leu Glu Asp Glu Cys Ser Glu Leu Lys Lys Asp Ile Asp Asp Leu
945 950 955 960
Glu Leu Thr Leu Ala Lys Val Glu Lys Glu Lys His Ala Thr Glu Asn
965 970 975
Lys Val Lys Asn Leu Thr Glu Glu Met Ala Gly Leu Asp Glu Thr Ile
980 985 990
Ala Lys Leu Thr Lys Glu Lys Lys Ala Leu Gln Glu Ala His Gln Gln
995 1000 1005
Thr Leu Asp Asp Leu Gln Ala Glu Glu Asp Lys Val Asn Thr Leu
1010 1015 1020
Thr Lys Ala Lys Thr Lys Leu Glu Gln Gln Val Asp Asp Leu Glu
1025 1030 1035
Gly Ser Leu Glu Gln Glu Lys Lys Leu Arg Met Asp Leu Glu Arg
1040 1045 1050
Ala Lys Arg Lys Leu Glu Gly Asp Leu Lys Leu Ala Gln Glu Ser
1055 1060 1065
Thr Met Asp Ile Glu Asn Asp Lys Gln Gln Leu Asp Glu Lys Leu
1070 1075 1080
Lys Lys Lys Glu Phe Glu Met Ser Asn Leu Gln Ser Lys Ile Glu
1085 1090 1095
Asp Glu Gln Ala Leu Ala Met Gln Leu Gln Lys Lys Ile Lys Glu
1100 1105 1110
Leu Gln Ala Arg Ile Glu Glu Leu Glu Glu Glu Ile Glu Ala Glu
1115 1120 1125
Arg Ala Ser Arg Ala Lys Ala Glu Lys Gln Arg Ser Asp Leu Ser
1130 1135 1140
Arg Glu Leu Glu Glu Ile Ser Glu Arg Leu Glu Glu Ala Gly Gly
1145 1150 1155
Ala Thr Ser Ala Gln Ile Glu Met Asn Lys Lys Arg Glu Ala Glu
1160 1165 1170
Phe Gln Lys Met Arg Arg Asp Leu Glu Glu Ala Thr Leu Gln His
1175 1180 1185
Glu Ala Thr Ala Ala Ala Leu Arg Lys Lys His Ala Asp Ser Val
1190 1195 1200
Ala Glu Leu Gly Glu Gln Ile Asp Asn Leu Gln Arg Val Lys Gln
1205 1210 1215
Lys Leu Glu Lys Glu Lys Ser Glu Met Lys Met Glu Ile Asp Asp
1220 1225 1230
Leu Ala Ser Asn Met Glu Thr Val Ser Lys Ala Lys Gly Asn Leu
1235 1240 1245
Glu Lys Met Cys Arg Ala Leu Glu Asp Gln Leu Ser Glu Leu Lys
1250 1255 1260
Thr Lys Glu Asp Glu Gln Gln Arg Leu Ile Asn Asp Leu Thr Thr
1265 1270 1275
Gln Arg Ala Arg Leu Gln Thr Glu Ser Gly Glu Phe Ser Arg Gln
1280 1285 1290
Leu Asp Glu Lys Asp Ala Leu Val Ser Gln Leu Ser Arg Gly Lys
1295 1300 1305
Gln Ala Phe Thr Gln Gln Ile Glu Glu Leu Lys Arg Gln Leu Glu
1310 1315 1320
Glu Glu Ile Lys Ala Lys Ser Ala Leu Ala His Ala Leu Gln Ser
1325 1330 1335
Ala Arg His Asp Cys Asp Leu Leu Arg Glu Gln Tyr Glu Glu Glu
1340 1345 1350
Gln Glu Gly Lys Ala Glu Leu Gln Arg Ala Met Ser Lys Ala Asn
1355 1360 1365
Ser Glu Val Ala Gln Trp Arg Thr Lys Tyr Glu Thr Asp Ala Ile
1370 1375 1380
Gln Arg Thr Glu Glu Leu Glu Glu Ala Lys Lys Lys Leu Ala Gln
1385 1390 1395
Arg Leu Gln Asp Ala Glu Glu His Val Glu Ala Val Asn Ala Lys
1400 1405 1410
Cys Ala Ser Leu Glu Lys Thr Lys Gln Arg Leu Gln Asn Glu Val
1415 1420 1425
Glu Asp Leu Met Ile Asp Val Glu Arg Thr Asn Ala Ala Cys Ala
1430 1435 1440
Ala Leu Asp Lys Lys Gln Arg Asn Phe Asp Lys Ile Leu Ser Glu
1445 1450 1455
Trp Lys Gln Lys Tyr Glu Glu Thr His Ala Glu Leu Glu Ala Ser
1460 1465 1470
Gln Lys Glu Ser Arg Ser Leu Ser Thr Glu Leu Phe Lys Ile Lys
1475 1480 1485
Asn Ala Tyr Glu Glu Ser Leu Asp Gln Leu Glu Thr Leu Lys Arg
1490 1495 1500
Glu Asn Lys Asn Leu Gln Gln Glu Ile Ser Asp Leu Thr Glu Gln
1505 1510 1515
Ile Ala Glu Gly Gly Lys Arg Ile His Glu Leu Glu Lys Val Lys
1520 1525 1530
Lys Gln Val Glu Gln Glu Lys Ser Glu Ile Gln Ala Ala Leu Glu
1535 1540 1545
Glu Ala Glu Ala Ser Leu Glu His Glu Glu Gly Lys Ile Leu Arg
1550 1555 1560
Ile Gln Leu Glu Leu Asn Gln Val Lys Ser Glu Ile Asp Arg Lys
1565 1570 1575
Ile Ala Glu Lys Asp Glu Glu Ile Asp Gln Leu Lys Arg Asn His
1580 1585 1590
Ile Arg Ile Val Glu Ser Met Gln Ser Thr Leu Asp Ala Glu Ile
1595 1600 1605
Arg Ser Arg Asn Asp Ala Ile Arg Leu Lys Lys Lys Met Glu Gly
1610 1615 1620
Asp Leu Asn Glu Met Glu Ile Gln Leu Asn His Ala Asn Arg Met
1625 1630 1635
Ala Ala Glu Ala Leu Lys Asn Tyr Arg Ser Thr Gln Ala Ile Leu
1640 1645 1650
Lys Asp Thr Gln Ile His Leu Asp Asp Ala Leu Arg Gly Gln Glu
1655 1660 1665
Asp Leu Lys Glu Gln Leu Ala Met Val Glu Arg Arg Ala Asn Leu
1670 1675 1680
Leu Gln Ala Glu Ile Glu Glu Leu Arg Ala Thr Leu Glu Gln Thr
1685 1690 1695
Glu Arg Ser Arg Lys Ile Ala Glu Gln Glu Leu Leu Asp Ala Ser
1700 1705 1710
Glu Arg Val Gln Leu Leu His Thr Gln Asn Thr Ser Leu Ile Asn
1715 1720 1725
Thr Lys Lys Lys Leu Glu Thr Asp Ile Thr Gln Ile Gln Gly Glu
1730 1735 1740
Met Glu Asp Ile Ile Gln Glu Ala Arg Asn Ala Glu Glu Lys Ala
1745 1750 1755
Lys Lys Ala Ile Thr Asp Ala Ala Met Met Ala Glu Glu Leu Lys
1760 1765 1770
Lys Glu Gln Asp Thr Ser Ala His Leu Glu Arg Met Lys Lys Asn
1775 1780 1785
Leu Glu Gln Thr Val Lys Asp Leu Gln His Arg Leu Asp Glu Ala
1790 1795 1800
Glu Gln Leu Ala Leu Lys Gly Gly Lys Lys Gln Ile Gln Lys Leu
1805 1810 1815
Glu Ala Arg Val Arg Glu Leu Glu Gly Glu Val Glu Ser Glu Gln
1820 1825 1830
Lys Arg Asn Val Glu Ala Val Lys Gly Leu Arg Lys His Glu Arg
1835 1840 1845
Arg Val Lys Glu Leu Thr Tyr Gln Thr Glu Glu Asp Arg Lys Asn
1850 1855 1860
Ile Leu Arg Leu Gln Asp Leu Val Asp Lys Leu Gln Ala Lys Val
1865 1870 1875
Lys Ser Tyr Lys Arg Gln Ala Glu Glu Ala Glu Glu Gln Ser Asn
1880 1885 1890
Val Asn Leu Ser Lys Phe Arg Lys Leu Gln His Glu Leu Glu Glu
1895 1900 1905
Ala Glu Glu Arg Ala Asp Ile Ala Glu Ser Gln Val Asn Lys Leu
1910 1915 1920
Arg Val Lys Ser Arg Glu Val His Thr Lys Ile Ile Ser Glu Glu
1925 1930 1935
<210> 6
<211> 1938
<212> PRT
<213> 普通牛
<400> 6
Met Ser Ser Asp Gln Glu Met Ala Ile Phe Gly Glu Ala Ala Pro Tyr
1 5 10 15
Leu Arg Lys Ser Glu Lys Glu Arg Ile Glu Ala Gln Asn Lys Pro Phe
20 25 30
Asp Ala Lys Thr Ser Val Phe Val Ala Asp Pro Lys Glu Ser Phe Val
35 40 45
Lys Ala Thr Val Gln Ser Arg Glu Gly Gly Lys Val Thr Ala Lys Thr
50 55 60
Glu Ala Gly Ala Thr Val Thr Val Lys Glu Asp Gln Val Phe Pro Met
65 70 75 80
Asn Pro Pro Lys Phe Asp Lys Ile Glu Asp Met Ala Met Met Thr His
85 90 95
Leu His Glu Pro Ala Val Leu Tyr Asn Leu Lys Glu Arg Tyr Ala Ala
100 105 110
Trp Met Ile Tyr Thr Tyr Ser Gly Leu Phe Cys Val Thr Val Asn Pro
115 120 125
Tyr Lys Trp Leu Pro Val Tyr Asn Ala Glu Val Val Thr Ala Tyr Arg
130 135 140
Gly Lys Lys Arg Gln Glu Ala Pro Pro His Ile Phe Ser Ile Ser Asp
145 150 155 160
Asn Ala Tyr Gln Phe Met Leu Thr Asp Arg Glu Asn Gln Ser Ile Leu
165 170 175
Ile Thr Gly Glu Ser Gly Ala Gly Lys Thr Val Asn Thr Lys Arg Val
180 185 190
Ile Gln Tyr Phe Ala Thr Ile Ala Val Thr Gly Glu Lys Lys Lys Glu
195 200 205
Glu Pro Thr Ser Gly Lys Met Gln Gly Thr Leu Glu Asp Gln Ile Ile
210 215 220
Ser Ala Asn Pro Leu Leu Glu Ala Phe Gly Asn Ala Lys Thr Val Arg
225 230 235 240
Asn Asp Asn Ser Ser Arg Phe Gly Lys Phe Ile Arg Ile His Phe Gly
245 250 255
Thr Thr Gly Lys Leu Ala Ser Ala Asp Ile Glu Thr Tyr Leu Leu Glu
260 265 270
Lys Ser Arg Val Thr Phe Gln Leu Lys Ala Glu Arg Ser Tyr His Ile
275 280 285
Phe Tyr Gln Ile Met Ser Asn Lys Lys Pro Glu Leu Ile Glu Met Leu
290 295 300
Leu Ile Thr Thr Asn Pro Tyr Asp Tyr Ala Tyr Val Ser Gln Gly Glu
305 310 315 320
Ile Thr Val Pro Ser Ile Asp Asp Gln Glu Glu Leu Met Ala Thr Asp
325 330 335
Ser Ala Ile Glu Ile Leu Gly Phe Thr Ser Asp Glu Arg Val Ser Ile
340 345 350
Tyr Lys Leu Thr Gly Ala Val Met His Tyr Gly Asn Leu Lys Phe Lys
355 360 365
Gln Lys Gln Arg Glu Glu Gln Ala Glu Pro Asp Gly Thr Glu Val Ala
370 375 380
Asp Lys Ala Ala Tyr Leu Gln Gly Leu Asn Ser Ala Asp Leu Leu Lys
385 390 395 400
Ala Leu Cys Tyr Pro Arg Val Lys Val Gly Asn Glu Phe Val Thr Lys
405 410 415
Gly Gln Thr Val Glu Gln Val Tyr Asn Ala Val Gly Ala Leu Ala Lys
420 425 430
Ala Val Tyr Asp Lys Met Phe Leu Trp Met Val Ala Arg Ile Asn Gln
435 440 445
Gln Leu Asp Thr Lys Gln Pro Arg Gln Tyr Phe Ile Gly Val Leu Asp
450 455 460
Ile Ala Gly Phe Glu Ile Phe Asp Phe Asn Ser Leu Glu Gln Leu Cys
465 470 475 480
Ile Asn Phe Thr Asn Glu Lys Leu Gln Gln Phe Phe Asn His His Met
485 490 495
Phe Val Leu Glu Gln Glu Glu Tyr Lys Lys Glu Gly Ile Glu Trp Glu
500 505 510
Phe Ile Asp Phe Gly Met Asp Leu Ala Ala Cys Ile Glu Leu Ile Glu
515 520 525
Lys Pro Met Gly Ile Phe Ser Ile Leu Glu Glu Glu Cys Met Phe Pro
530 535 540
Lys Ala Thr Asp Met Ser Phe Lys Asn Lys Leu Tyr Glu Gln His Leu
545 550 555 560
Gly Lys Ser Asn Asn Phe Gln Lys Pro Lys Pro Ala Lys Gly Lys Ala
565 570 575
Glu Ala His Phe Ser Leu Ile His Tyr Ala Gly Thr Val Asp Tyr Asn
580 585 590
Ile Thr Gly Trp Leu Asp Lys Asn Lys Asp Pro Leu Asn Glu Thr Val
595 600 605
Val Gly Leu Tyr Gln Lys Ser Ser Val Lys Thr Leu Ala Leu Leu Phe
610 615 620
Ser Gly Pro Ala Ser Gly Glu Ala Glu Gly Gly Pro Lys Lys Gly Gly
625 630 635 640
Lys Lys Lys Gly Ser Ser Phe Gln Thr Val Ser Ala Leu Phe Arg Glu
645 650 655
Asn Leu Asn Lys Leu Met Thr Asn Leu Arg Ser Thr His Pro His Phe
660 665 670
Val Arg Cys Ile Ile Pro Asn Glu Thr Lys Thr Pro Gly Ala Met Glu
675 680 685
His Glu Leu Val Leu His Gln Leu Arg Cys Asn Gly Val Leu Glu Gly
690 695 700
Ile Arg Ile Cys Arg Lys Gly Phe Pro Ser Arg Ile Leu Tyr Ala Asp
705 710 715 720
Phe Lys Gln Arg Tyr Lys Val Leu Asn Ala Ser Ala Ile Pro Glu Gly
725 730 735
Gln Phe Ile Asp Ser Lys Lys Ala Ser Glu Lys Leu Leu Ala Ser Ile
740 745 750
Asp Val Asp His Thr Gln Tyr Lys Phe Gly His Thr Lys Val Phe Phe
755 760 765
Lys Ala Gly Leu Leu Gly Leu Leu Glu Glu Met Arg Asp Glu Lys Leu
770 775 780
Ala Gln Leu Ile Thr Arg Thr Gln Ala Arg Cys Arg Gly Phe Leu Ala
785 790 795 800
Arg Val Glu Tyr Gln Lys Met Val Glu Arg Arg Glu Ser Ile Phe Cys
805 810 815
Ile Gln Tyr Asn Val Arg Ala Phe Met Asn Val Lys His Trp Pro Trp
820 825 830
Met Lys Leu Tyr Phe Lys Ile Lys Pro Leu Leu Lys Ser Ala Glu Thr
835 840 845
Glu Lys Glu Met Ala Asn Met Lys Glu Glu Phe Glu Lys Thr Lys Glu
850 855 860
Glu Leu Ala Lys Ser Glu Ala Lys Arg Lys Glu Leu Glu Glu Lys Met
865 870 875 880
Val Thr Leu Thr Gln Glu Lys Asn Asp Leu Gln Leu Gln Val Gln Ser
885 890 895
Glu Ala Asp Ala Leu Ala Asp Ala Glu Glu Arg Cys Asp Gln Leu Ile
900 905 910
Lys Thr Lys Ile Gln Leu Glu Ala Lys Ile Lys Glu Val Thr Glu Arg
915 920 925
Ala Glu Asp Glu Glu Glu Ile Asn Ala Glu Leu Thr Ala Lys Lys Arg
930 935 940
Lys Leu Glu Asp Glu Cys Ser Glu Leu Lys Lys Asp Ile Asp Asp Leu
945 950 955 960
Glu Leu Thr Leu Ala Lys Val Glu Lys Glu Lys His Ala Thr Glu Asn
965 970 975
Lys Val Lys Asn Leu Thr Glu Glu Met Ala Gly Leu Asp Glu Thr Ile
980 985 990
Ala Lys Leu Thr Lys Glu Lys Lys Ala Leu Gln Glu Ala His Gln Gln
995 1000 1005
Thr Leu Asp Asp Leu Gln Ala Glu Glu Asp Lys Val Asn Thr Leu
1010 1015 1020
Thr Lys Ala Lys Thr Lys Leu Glu Gln Gln Val Asp Asp Leu Glu
1025 1030 1035
Gly Ser Leu Glu Gln Glu Lys Lys Leu Arg Met Asp Leu Glu Arg
1040 1045 1050
Ala Lys Arg Lys Leu Glu Gly Asp Leu Lys Leu Ala Gln Glu Ser
1055 1060 1065
Thr Met Asp Ile Glu Asn Asp Lys Gln Gln Leu Asp Glu Lys Leu
1070 1075 1080
Lys Lys Lys Glu Phe Glu Met Ser Asn Leu Gln Ser Lys Ile Glu
1085 1090 1095
Asp Glu Gln Ala Leu Ala Met Gln Leu Gln Lys Lys Ile Lys Glu
1100 1105 1110
Leu Gln Ala Arg Ile Glu Glu Leu Glu Glu Glu Ile Glu Ala Glu
1115 1120 1125
Arg Ala Ser Arg Ala Lys Ala Glu Lys Gln Arg Ser Asp Leu Ser
1130 1135 1140
Arg Glu Leu Glu Glu Ile Ser Glu Arg Leu Glu Glu Ala Gly Gly
1145 1150 1155
Ala Thr Ser Ala Gln Ile Glu Met Asn Lys Lys Arg Glu Ala Glu
1160 1165 1170
Phe Gln Lys Met Arg Arg Asp Leu Glu Glu Ala Thr Leu Gln His
1175 1180 1185
Glu Ala Thr Ala Ala Ala Leu Arg Lys Lys His Ala Asp Ser Val
1190 1195 1200
Ala Glu Leu Gly Glu Gln Ile Asp Asn Leu Gln Arg Val Lys Gln
1205 1210 1215
Lys Leu Glu Lys Glu Lys Ser Glu Met Lys Met Glu Ile Asp Asp
1220 1225 1230
Leu Ala Ser Asn Met Glu Thr Val Ser Lys Ala Lys Gly Asn Leu
1235 1240 1245
Glu Lys Met Cys Arg Ala Leu Glu Asp Gln Leu Ser Glu Leu Lys
1250 1255 1260
Thr Lys Glu Asp Glu Gln Gln Arg Leu Ile Asn Asp Leu Thr Thr
1265 1270 1275
Gln Arg Ala Arg Leu Gln Thr Glu Ser Gly Glu Phe Ser Arg Gln
1280 1285 1290
Leu Asp Glu Lys Asp Ala Leu Val Ser Gln Leu Ser Arg Gly Lys
1295 1300 1305
Gln Ala Phe Thr Gln Gln Ile Glu Glu Leu Lys Arg Gln Leu Glu
1310 1315 1320
Glu Glu Ile Lys Ala Lys Ser Ala Leu Ala His Ala Leu Gln Ser
1325 1330 1335
Ala Arg His Asp Cys Asp Leu Leu Arg Glu Gln Tyr Glu Glu Glu
1340 1345 1350
Gln Glu Gly Lys Ala Glu Leu Gln Arg Ala Met Ser Lys Ala Asn
1355 1360 1365
Ser Glu Val Ala Gln Trp Arg Thr Lys Tyr Glu Thr Asp Ala Ile
1370 1375 1380
Gln Arg Thr Glu Glu Leu Glu Glu Ala Lys Lys Lys Leu Ala Gln
1385 1390 1395
Arg Leu Gln Asp Ala Glu Glu His Val Glu Ala Val Asn Ala Lys
1400 1405 1410
Cys Ala Ser Leu Glu Lys Thr Lys Gln Arg Leu Gln Asn Glu Val
1415 1420 1425
Glu Asp Leu Met Ile Asp Val Glu Arg Thr Asn Ala Ala Cys Ala
1430 1435 1440
Ala Leu Asp Lys Lys Gln Arg Asn Phe Asp Lys Ile Leu Ser Glu
1445 1450 1455
Trp Lys Gln Lys Tyr Glu Glu Thr His Ala Glu Leu Glu Ala Ser
1460 1465 1470
Gln Lys Glu Ser Arg Ser Leu Ser Thr Glu Leu Phe Lys Ile Lys
1475 1480 1485
Asn Ala Tyr Glu Glu Ser Leu Asp Gln Leu Glu Thr Leu Lys Arg
1490 1495 1500
Glu Asn Lys Asn Leu Gln Gln Glu Ile Ser Asp Leu Thr Glu Gln
1505 1510 1515
Ile Ala Glu Gly Gly Lys Arg Ile His Glu Leu Glu Lys Val Lys
1520 1525 1530
Lys Gln Val Glu Gln Glu Lys Ser Glu Ile Gln Ala Ala Leu Glu
1535 1540 1545
Glu Ala Glu Ala Ser Leu Glu His Glu Glu Gly Lys Ile Leu Arg
1550 1555 1560
Ile Gln Leu Glu Leu Asn Gln Val Lys Ser Glu Ile Asp Arg Lys
1565 1570 1575
Ile Ala Glu Lys Asp Glu Glu Ile Asp Gln Leu Lys Arg Asn His
1580 1585 1590
Ile Arg Val Val Glu Ser Met Gln Ser Thr Leu Asp Ala Glu Ile
1595 1600 1605
Arg Ser Arg Asn Asp Ala Ile Arg Leu Lys Lys Lys Met Glu Gly
1610 1615 1620
Asp Leu Asn Glu Met Glu Ile Gln Leu Asn His Ala Asn Arg Met
1625 1630 1635
Ala Ala Glu Ala Leu Lys Asn Tyr Arg Asn Thr Gln Ala Ile Leu
1640 1645 1650
Lys Asp Thr Gln Ile His Leu Asp Asp Ala Leu Arg Gly Gln Glu
1655 1660 1665
Asp Leu Lys Glu Gln Leu Ala Met Val Glu Arg Arg Ala Asn Leu
1670 1675 1680
Leu Gln Ala Glu Ile Glu Glu Leu Arg Ala Thr Leu Glu Gln Thr
1685 1690 1695
Glu Arg Ser Arg Lys Ile Ala Glu Gln Glu Leu Leu Asp Ala Ser
1700 1705 1710
Glu Arg Val Gln Leu Leu His Thr Gln Asn Thr Ser Leu Ile Asn
1715 1720 1725
Thr Lys Lys Lys Leu Glu Thr Asp Ile Thr Gln Ile Gln Gly Glu
1730 1735 1740
Met Glu Asp Ile Ile Gln Glu Ala Arg Asn Ala Glu Glu Lys Ala
1745 1750 1755
Lys Lys Ala Ile Thr Asp Ala Ala Met Met Ala Glu Glu Leu Lys
1760 1765 1770
Lys Glu Gln Asp Thr Ser Ala His Leu Glu Arg Met Lys Lys Asn
1775 1780 1785
Leu Glu Gln Thr Val Lys Asp Leu Gln His Arg Leu Asp Glu Ala
1790 1795 1800
Glu Gln Leu Ala Leu Lys Gly Gly Lys Lys Gln Ile Gln Lys Leu
1805 1810 1815
Glu Ala Arg Val Arg Glu Leu Glu Gly Glu Val Glu Ser Glu Gln
1820 1825 1830
Lys Arg Asn Val Glu Ala Val Lys Gly Leu Arg Lys His Glu Arg
1835 1840 1845
Arg Val Lys Glu Leu Thr Tyr Gln Thr Glu Glu Asp Arg Lys Asn
1850 1855 1860
Ile Leu Arg Leu Gln Asp Leu Val Asp Lys Leu Gln Ala Lys Val
1865 1870 1875
Lys Ser Tyr Lys Arg Gln Ala Glu Glu Ala Glu Glu Gln Ser Asn
1880 1885 1890
Val Asn Leu Ser Lys Phe Arg Lys Leu Gln His Glu Leu Glu Glu
1895 1900 1905
Ala Glu Glu Arg Ala Asp Ile Ala Glu Ser Gln Val Asn Lys Leu
1910 1915 1920
Arg Val Lys Ser Arg Glu Val His Thr Lys Ile Ile Ser Glu Glu
1925 1930 1935
<210> 7
<211> 1939
<212> PRT
<213> 野猪
<400> 7
Met Ser Ser Asp Gln Glu Met Ala Ile Phe Gly Glu Ala Ala Pro Tyr
1 5 10 15
Leu Arg Lys Ser Glu Lys Glu Arg Ile Glu Ala Gln Asn Lys Pro Phe
20 25 30
Asp Ala Lys Thr Ser Val Phe Val Ala Glu Pro Lys Glu Ser Phe Val
35 40 45
Lys Gly Thr Val Gln Ser Arg Glu Gly Gly Lys Val Thr Val Lys Thr
50 55 60
Glu Ala Gly Ala Thr Leu Thr Val Lys Glu Asp Gln Val Phe Pro Met
65 70 75 80
Asn Pro Pro Lys Phe Asp Lys Ile Glu Asp Met Ala Met Met Thr His
85 90 95
Leu His Glu Pro Ala Val Leu Tyr Asn Leu Lys Glu Arg Tyr Ala Ala
100 105 110
Trp Met Ile Tyr Thr Tyr Ser Gly Leu Phe Cys Val Thr Val Asn Pro
115 120 125
Tyr Lys Trp Leu Pro Val Tyr Asn Ala Glu Val Val Thr Ala Tyr Arg
130 135 140
Gly Lys Lys Arg Gln Glu Ala Pro Pro His Ile Phe Ser Ile Ser Asp
145 150 155 160
Asn Ala Tyr Gln Phe Met Leu Thr Asp Arg Glu Asn Gln Ser Ile Leu
165 170 175
Ile Thr Gly Glu Ser Gly Ala Gly Lys Thr Val Asn Thr Lys Arg Val
180 185 190
Ile Gln Tyr Phe Ala Thr Ile Ala Val Thr Gly Glu Lys Lys Lys Glu
195 200 205
Glu Pro Thr Ser Gly Lys Met Gln Gly Thr Leu Glu Asp Gln Ile Ile
210 215 220
Ser Ala Asn Pro Leu Leu Glu Ala Phe Gly Asn Ala Lys Thr Val Arg
225 230 235 240
Asn Asp Asn Ser Ser Arg Phe Gly Lys Phe Ile Arg Ile His Phe Gly
245 250 255
Thr Thr Gly Lys Leu Ala Ser Ala Asp Ile Glu Thr Tyr Leu Leu Glu
260 265 270
Lys Ser Arg Val Thr Phe Gln Leu Lys Ala Glu Arg Ser Tyr His Ile
275 280 285
Phe Tyr Gln Ile Met Ser Asn Lys Lys Pro Glu Leu Ile Glu Met Leu
290 295 300
Leu Ile Thr Thr Asn Pro Tyr Asp Tyr Ala Phe Val Ser Gln Gly Glu
305 310 315 320
Ile Thr Val Pro Ser Ile Asp Asp Gln Glu Glu Leu Met Ala Thr Asp
325 330 335
Ser Ala Ile Glu Ile Leu Gly Phe Thr Ser Asp Glu Arg Val Ser Ile
340 345 350
Tyr Lys Leu Thr Gly Ala Val Met His Tyr Gly Asn Leu Lys Phe Lys
355 360 365
Gln Lys Gln Arg Glu Glu Gln Ala Glu Pro Asp Gly Thr Glu Val Ala
370 375 380
Asp Lys Ala Ala Tyr Leu Gln Gly Leu Asn Ser Ala Asp Leu Leu Lys
385 390 395 400
Ala Leu Cys Tyr Pro Arg Val Lys Val Gly Asn Glu Phe Val Thr Lys
405 410 415
Gly Gln Thr Val Gln Gln Val Tyr Asn Ala Val Gly Ala Leu Ala Lys
420 425 430
Ala Val Tyr Asp Lys Met Phe Leu Trp Met Val Thr Arg Ile Asn Gln
435 440 445
Gln Leu Asp Thr Lys Gln Pro Arg Gln Tyr Phe Ile Gly Val Leu Asp
450 455 460
Ile Ala Gly Phe Glu Ile Phe Asp Phe Asn Ser Leu Glu Gln Leu Cys
465 470 475 480
Ile Asn Phe Thr Asn Glu Lys Leu Gln Gln Phe Phe Asn His His Met
485 490 495
Phe Val Leu Glu Gln Glu Glu Tyr Lys Lys Glu Gly Ile Glu Trp Glu
500 505 510
Phe Ile Asp Phe Gly Met Asp Leu Ala Ala Cys Ile Glu Leu Ile Glu
515 520 525
Lys Pro Met Gly Ile Phe Ser Ile Leu Glu Glu Glu Cys Met Phe Pro
530 535 540
Lys Ala Thr Asp Thr Ser Phe Lys Asn Lys Leu Tyr Glu Gln His Leu
545 550 555 560
Gly Lys Ser Asn Asn Phe Gln Lys Pro Lys Pro Ala Lys Gly Lys Val
565 570 575
Glu Ala His Phe Ser Leu Ile His Tyr Ala Gly Thr Val Asp Tyr Asn
580 585 590
Ile Thr Gly Trp Leu Asp Lys Asn Lys Asp Pro Leu Asn Glu Thr Val
595 600 605
Val Gly Leu Tyr Gln Lys Ser Ser Val Lys Thr Leu Ala Phe Leu Phe
610 615 620
Thr Gly Ala Ala Gly Ala Asp Ala Glu Ala Gly Gly Gly Lys Lys Gly
625 630 635 640
Gly Lys Lys Lys Gly Ser Ser Phe Gln Thr Val Ser Ala Leu Phe Arg
645 650 655
Glu Asn Leu Asn Lys Leu Met Thr Asn Leu Arg Ser Thr His Pro His
660 665 670
Phe Val Arg Cys Ile Ile Pro Asn Glu Thr Lys Thr Pro Gly Ala Met
675 680 685
Glu His Glu Leu Val Leu His Gln Leu Arg Cys Asn Gly Val Leu Glu
690 695 700
Gly Ile Arg Ile Cys Arg Lys Gly Phe Pro Ser Arg Ile Leu Tyr Ala
705 710 715 720
Asp Phe Lys Gln Arg Tyr Lys Val Leu Asn Ala Ser Ala Ile Pro Glu
725 730 735
Gly Gln Phe Ile Asp Ser Lys Lys Ala Ser Glu Lys Leu Leu Gly Ser
740 745 750
Ile Asp Ile Asp His Thr Gln Tyr Lys Phe Gly His Thr Lys Val Phe
755 760 765
Phe Lys Ala Gly Leu Leu Gly Leu Leu Glu Glu Met Arg Asp Glu Lys
770 775 780
Leu Ala Gln Leu Ile Thr Arg Thr Gln Ala Arg Cys Arg Gly Phe Leu
785 790 795 800
Ala Arg Val Glu Tyr Gln Lys Met Val Glu Arg Arg Glu Ser Ile Phe
805 810 815
Cys Ile Gln Tyr Asn Ile Arg Ala Phe Met Asn Val Lys His Trp Pro
820 825 830
Trp Met Lys Leu Tyr Phe Lys Ile Lys Pro Leu Leu Lys Ser Ala Glu
835 840 845
Thr Glu Lys Glu Met Ala Asn Met Lys Glu Glu Phe Glu Lys Thr Lys
850 855 860
Glu Ser Leu Ala Lys Ala Glu Ala Lys Arg Lys Glu Leu Glu Glu Lys
865 870 875 880
Met Val Ala Leu Met Gln Glu Lys Asn Asp Leu Gln Leu Gln Val Gln
885 890 895
Ala Glu Ala Asp Ser Leu Ala Asp Ala Glu Glu Arg Cys Asp Gln Leu
900 905 910
Ile Lys Thr Lys Ile Gln Leu Glu Ala Lys Ile Lys Glu Val Thr Glu
915 920 925
Arg Ala Glu Asp Glu Glu Glu Ile Asn Ala Glu Leu Thr Ala Lys Lys
930 935 940
Arg Lys Leu Glu Asp Glu Cys Ser Glu Leu Lys Lys Asp Ile Asp Asp
945 950 955 960
Leu Glu Leu Thr Leu Ala Lys Val Glu Lys Glu Lys His Ala Thr Glu
965 970 975
Asn Lys Val Lys Asn Leu Thr Glu Glu Met Ala Gly Leu Asp Glu Thr
980 985 990
Ile Ala Lys Leu Thr Lys Glu Lys Lys Ala Leu Gln Glu Ala His Gln
995 1000 1005
Gln Thr Leu Asp Asp Leu Gln Ala Glu Glu Asp Lys Val Asn Thr
1010 1015 1020
Leu Thr Lys Ala Lys Thr Lys Leu Glu Gln Gln Val Asp Asp Leu
1025 1030 1035
Glu Gly Ser Leu Glu Gln Glu Lys Lys Leu Arg Met Asp Leu Glu
1040 1045 1050
Arg Ala Lys Arg Lys Leu Glu Gly Asp Leu Lys Leu Ala Gln Glu
1055 1060 1065
Ser Thr Met Asp Ile Glu Asn Asp Lys Gln Gln Leu Asp Glu Lys
1070 1075 1080
Leu Lys Lys Lys Glu Phe Glu Met Ser Asn Leu Gln Ser Lys Ile
1085 1090 1095
Glu Asp Glu Gln Ala Leu Ala Met Gln Leu Gln Lys Lys Ile Lys
1100 1105 1110
Glu Leu Gln Ala Arg Ile Glu Glu Leu Glu Glu Glu Ile Glu Ala
1115 1120 1125
Glu Arg Ala Ser Arg Ala Lys Ala Glu Lys Gln Arg Ser Asp Leu
1130 1135 1140
Ser Arg Glu Leu Glu Glu Ile Ser Glu Arg Leu Glu Glu Ala Gly
1145 1150 1155
Gly Ala Thr Ser Ala Gln Ile Glu Met Asn Lys Lys Arg Glu Ala
1160 1165 1170
Glu Phe Gln Lys Met Arg Arg Asp Leu Glu Glu Ala Thr Leu Gln
1175 1180 1185
His Glu Ala Thr Ala Ala Thr Leu Arg Lys Lys His Ala Asp Ser
1190 1195 1200
Val Ala Glu Leu Gly Glu Gln Ile Asp Asn Leu Gln Arg Val Lys
1205 1210 1215
Gln Lys Leu Glu Lys Glu Lys Ser Glu Met Lys Met Glu Ile Asp
1220 1225 1230
Asp Leu Ala Ser Asn Met Glu Thr Val Ser Lys Ala Lys Gly Asn
1235 1240 1245
Leu Glu Lys Met Cys Arg Thr Leu Glu Asp Gln Leu Ser Glu Leu
1250 1255 1260
Lys Thr Lys Glu Glu Glu Gln Gln Arg Leu Ile Asn Asp Leu Thr
1265 1270 1275
Ala Gln Arg Ala Arg Leu Gln Thr Glu Ser Gly Glu Tyr Ser Arg
1280 1285 1290
Gln Leu Asp Glu Lys Asp Thr Leu Val Ser Gln Leu Ser Arg Gly
1295 1300 1305
Lys Gln Ala Phe Thr Gln Gln Ile Glu Glu Leu Lys Arg Gln Leu
1310 1315 1320
Glu Glu Glu Ile Lys Ala Lys Ser Ala Leu Ala His Ala Val Gln
1325 1330 1335
Ser Ser Arg His Asp Cys Asp Leu Leu Arg Glu Gln Tyr Glu Glu
1340 1345 1350
Glu Gln Glu Ala Lys Ala Glu Leu Gln Arg Ala Met Ser Lys Ala
1355 1360 1365
Asn Ser Glu Val Ala Gln Trp Arg Thr Lys Tyr Glu Thr Asp Ala
1370 1375 1380
Ile Gln Arg Thr Glu Glu Leu Glu Glu Ala Lys Lys Lys Leu Ala
1385 1390 1395
Gln Arg Leu Gln Asp Ala Glu Glu His Val Glu Ala Val Asn Ala
1400 1405 1410
Lys Cys Ala Ser Leu Glu Lys Thr Lys Gln Arg Leu Gln Asn Glu
1415 1420 1425
Val Glu Asp Leu Met Ile Asp Val Glu Arg Ser Asn Ala Ala Cys
1430 1435 1440
Ala Ala Leu Asp Lys Lys Gln Arg Asn Phe Asp Lys Ile Leu Ala
1445 1450 1455
Glu Trp Lys Gln Lys Tyr Glu Glu Thr His Ala Glu Leu Glu Ala
1460 1465 1470
Ser Gln Lys Glu Ser Arg Ser Leu Ser Thr Glu Leu Phe Lys Val
1475 1480 1485
Lys Asn Ala Tyr Glu Glu Ser Leu Asp Gln Leu Glu Thr Leu Lys
1490 1495 1500
Arg Glu Asn Lys Asn Leu Gln Gln Glu Ile Ser Asp Leu Thr Glu
1505 1510 1515
Gln Ile Ala Glu Gly Gly Lys Arg Ile His Glu Leu Glu Lys Ile
1520 1525 1530
Lys Lys Gln Val Glu Gln Glu Lys Ser Glu Ile Gln Ala Ala Leu
1535 1540 1545
Glu Glu Ala Glu Ala Ser Leu Glu His Glu Glu Gly Lys Ile Leu
1550 1555 1560
Arg Ile Gln Leu Glu Leu Asn Gln Val Lys Ser Glu Val Asp Arg
1565 1570 1575
Lys Ile Ala Glu Lys Asp Glu Glu Ile Asp Gln Leu Lys Arg Asn
1580 1585 1590
His Val Arg Val Val Glu Ser Met Gln Ser Met Leu Asp Ala Glu
1595 1600 1605
Ile Arg Ser Arg Asn Asp Ala Ile Arg Leu Lys Lys Lys Met Glu
1610 1615 1620
Gly Asp Leu Asn Glu Met Glu Ile Gln Leu Asn His Ala Asn Arg
1625 1630 1635
Met Ala Ala Glu Ala Leu Arg Asn Tyr Arg Asn Thr Gln Gly Ile
1640 1645 1650
Leu Lys Asp Thr Gln Ile His Leu Asp Asp Ala Leu Arg Ser Gln
1655 1660 1665
Glu Asp Leu Lys Glu Gln Leu Ala Met Val Glu Arg Arg Ala Asn
1670 1675 1680
Leu Leu Gln Ala Glu Ile Glu Glu Leu Arg Ala Thr Leu Glu Gln
1685 1690 1695
Thr Glu Arg Ser Arg Lys Val Ala Glu Gln Glu Leu Leu Asp Ala
1700 1705 1710
Ser Glu Arg Val Gln Leu Leu His Thr Gln Asn Thr Ser Leu Ile
1715 1720 1725
Asn Thr Lys Lys Lys Leu Glu Thr Asp Ile Ser Gln Ile Gln Gly
1730 1735 1740
Glu Met Glu Asp Ile Ile Gln Glu Ala Arg Asn Ala Glu Glu Lys
1745 1750 1755
Ala Lys Lys Ala Ile Thr Asp Ala Ala Met Met Ala Glu Glu Leu
1760 1765 1770
Lys Lys Glu Gln Asp Thr Ser Ala His Leu Glu Arg Met Lys Lys
1775 1780 1785
Asn Leu Glu Gln Thr Val Lys Asp Leu Gln His Arg Leu Asp Glu
1790 1795 1800
Ala Glu Gln Leu Ala Leu Lys Gly Gly Lys Lys Gln Ile Gln Lys
1805 1810 1815
Leu Glu Ala Arg Val Arg Glu Leu Glu Gly Glu Val Glu Ser Glu
1820 1825 1830
Gln Lys Arg Asn Val Glu Thr Val Lys Gly Leu Arg Lys His Glu
1835 1840 1845
Arg Arg Val Lys Glu Leu Thr Tyr Gln Thr Glu Glu Asp Arg Lys
1850 1855 1860
Asn Ile Leu Arg Leu Gln Asp Leu Val Asp Lys Leu Gln Ala Lys
1865 1870 1875
Val Lys Ser Tyr Lys Arg Gln Ala Glu Glu Ala Glu Glu Gln Ser
1880 1885 1890
Asn Val Asn Leu Ser Lys Phe Arg Lys Leu Gln His Glu Leu Glu
1895 1900 1905
Glu Ala Glu Glu Arg Ala Asp Ile Ala Glu Ser Gln Val Asn Lys
1910 1915 1920
Leu Arg Val Lys Ser Arg Glu Val His Thr Lys Ile Ile Ser Glu
1925 1930 1935
Glu
<210> 8
<211> 1939
<212> PRT
<213> 绵羊
<400> 8
Met Ser Ser Asp Gln Glu Met Ala Ile Phe Gly Glu Ala Ala Pro Tyr
1 5 10 15
Leu Arg Lys Ser Glu Lys Glu Arg Ile Glu Ala Gln Asn Lys Pro Phe
20 25 30
Asp Ala Lys Thr Ser Val Phe Val Ala Asp Pro Lys Glu Ser Phe Val
35 40 45
Lys Ala Thr Val Gln Ser Arg Glu Gly Gly Lys Val Thr Ala Lys Thr
50 55 60
Glu Ala Gly Ala Thr Val Thr Val Lys Glu Asp Gln Val Phe Pro Met
65 70 75 80
Asn Pro Pro Lys Phe Asp Lys Ile Glu Asp Met Ala Met Met Thr His
85 90 95
Leu His Glu Pro Gly Val Leu Tyr Asn Leu Lys Glu Arg Tyr Ala Ala
100 105 110
Trp Met Ile Tyr Thr Tyr Ser Gly Leu Phe Cys Val Thr Val Asn Pro
115 120 125
Tyr Lys Trp Leu Pro Val Tyr Asn Ala Glu Val Val Ala Ala Tyr Arg
130 135 140
Gly Lys Lys Arg Gln Glu Ala Pro Pro His Ile Phe Ser Ile Ser Asp
145 150 155 160
Asn Ala Tyr Gln Phe Met Leu Thr Asp Arg Glu Asn Gln Ser Ile Leu
165 170 175
Ile Thr Gly Glu Ser Gly Ala Gly Lys Thr Val Asn Thr Lys Arg Val
180 185 190
Ile Gln Tyr Phe Ala Thr Ile Ala Val Thr Gly Glu Lys Lys Lys Glu
195 200 205
Glu Pro Thr Ser Gly Lys Met Gln Gly Thr Leu Glu Asp Gln Ile Ile
210 215 220
Ser Ala Asn Pro Leu Leu Glu Ala Phe Gly Asn Ala Lys Thr Val Arg
225 230 235 240
Asn Asp Asn Ser Ser Arg Phe Gly Lys Phe Ile Arg Ile His Phe Gly
245 250 255
Thr Thr Gly Lys Leu Ala Ser Ala Asp Ile Glu Thr Tyr Leu Leu Glu
260 265 270
Lys Ser Arg Val Thr Phe Gln Leu Lys Ala Glu Arg Ser Tyr His Ile
275 280 285
Phe Tyr Gln Ile Met Ser Asn Lys Lys Pro Glu Leu Ile Glu Met Leu
290 295 300
Leu Ile Thr Thr Asn Pro Tyr Asp Tyr Ala Phe Val Ser Gln Gly Glu
305 310 315 320
Ile Thr Val Pro Ser Ile Asp Asp Gln Glu Glu Leu Met Ala Thr Asp
325 330 335
Ser Ala Ile Glu Ile Leu Gly Phe Thr Ser Asp Glu Arg Val Ser Ile
340 345 350
Tyr Lys Leu Thr Gly Ala Val Met His Tyr Gly Asn Leu Lys Phe Lys
355 360 365
Gln Lys Gln Arg Glu Glu Gln Ala Glu Pro Asp Gly Thr Glu Val Ala
370 375 380
Asp Lys Ala Ala Tyr Leu Gln Gly Leu Asn Ser Ala Asp Leu Leu Lys
385 390 395 400
Ala Leu Cys Tyr Pro Arg Val Lys Val Gly Asn Glu Phe Val Thr Lys
405 410 415
Gly Gln Thr Val Glu Gln Val Tyr Asn Ala Val Gly Ala Leu Ala Lys
420 425 430
Ala Ile Tyr Asp Lys Met Phe Leu Trp Met Val Thr Arg Ile Asn Gln
435 440 445
Gln Leu Asp Thr Lys Gln Pro Arg Gln Tyr Phe Ile Gly Val Leu Asp
450 455 460
Ile Ala Gly Phe Glu Ile Phe Asp Phe Asn Ser Phe Glu Gln Leu Cys
465 470 475 480
Ile Asn Phe Thr Asn Glu Lys Leu Gln Gln Phe Phe Asn His His Met
485 490 495
Phe Val Leu Glu Gln Glu Glu Tyr Lys Arg Glu Gly Ile Glu Trp Glu
500 505 510
Phe Ile Asp Phe Gly Met Asp Leu Ala Ala Cys Ile Glu Leu Ile Glu
515 520 525
Lys Pro Met Gly Ile Phe Ser Ile Leu Glu Glu Glu Cys Met Phe Pro
530 535 540
Lys Ala Thr Asp Thr Ser Phe Lys Asn Lys Leu Tyr Glu Gln His Leu
545 550 555 560
Gly Lys Ser Asn Asn Phe Gln Lys Pro Lys Pro Ala Lys Gly Lys Ala
565 570 575
Glu Ala His Phe Ser Leu Ile His Tyr Ala Gly Thr Val Asp Tyr Asn
580 585 590
Ile Thr Gly Trp Leu Asp Lys Asn Lys Asp Pro Leu Asn Glu Thr Val
595 600 605
Val Gly Leu Tyr Gln Lys Ser Ser Val Lys Thr Leu Ala Phe Leu Phe
610 615 620
Ser Gly Ala Ala Ser Ala Glu Ala Glu Gly Gly Gly Ala Lys Lys Gly
625 630 635 640
Gly Lys Lys Lys Gly Ser Ser Phe Gln Thr Val Ser Ala Leu Phe Arg
645 650 655
Glu Asn Leu Asn Lys Leu Met Thr Asn Leu Arg Ser Thr His Pro His
660 665 670
Phe Val Arg Cys Ile Ile Pro Asn Glu Thr Lys Thr Pro Gly Ala Met
675 680 685
Glu His Glu Leu Val Leu His Gln Leu Arg Cys Asn Gly Val Leu Glu
690 695 700
Gly Ile Arg Ile Cys Arg Lys Gly Phe Pro Ser Arg Ile Leu Tyr Ala
705 710 715 720
Asp Phe Lys Gln Arg Tyr Lys Val Leu Asn Ala Ser Ala Ile Pro Glu
725 730 735
Gly Gln Phe Ile Asp Ser Lys Lys Ala Ser Glu Lys Leu Leu Gly Ser
740 745 750
Ile Asp Val Asp His Thr Gln Tyr Lys Phe Gly His Thr Lys Val Phe
755 760 765
Phe Lys Ala Gly Leu Leu Gly Leu Leu Glu Glu Met Arg Asp Glu Lys
770 775 780
Leu Ala Gln Leu Ile Thr Arg Thr Gln Ala Arg Cys Arg Gly Phe Leu
785 790 795 800
Ala Arg Val Glu Tyr Gln Arg Met Val Glu Arg Arg Glu Ser Ile Phe
805 810 815
Cys Ile Gln Tyr Asn Val Arg Ala Phe Met Asn Val Lys His Trp Pro
820 825 830
Trp Met Lys Leu Tyr Phe Lys Ile Lys Pro Leu Leu Lys Ser Ala Glu
835 840 845
Thr Glu Lys Glu Met Ala Asn Met Lys Glu Glu Phe Glu Lys Thr Lys
850 855 860
Glu Glu Leu Ala Lys Ser Glu Ala Lys Arg Lys Glu Leu Glu Glu Lys
865 870 875 880
Met Val Thr Leu Thr Gln Glu Lys Asn Asp Leu Gln Leu Gln Val Gln
885 890 895
Ser Glu Ala Asp Ala Leu Ala Asp Ala Glu Glu Arg Cys Asp Gln Leu
900 905 910
Ile Lys Thr Lys Ile Gln Leu Glu Ala Lys Ile Lys Glu Val Thr Glu
915 920 925
Arg Ala Glu Asp Glu Glu Glu Ile Asn Ala Glu Leu Thr Ala Lys Lys
930 935 940
Arg Lys Leu Glu Asp Glu Cys Ser Glu Leu Lys Lys Asp Ile Asp Asp
945 950 955 960
Leu Glu Leu Thr Leu Ala Lys Val Glu Lys Glu Lys His Ala Thr Glu
965 970 975
Asn Lys Val Lys Asn Leu Thr Glu Glu Met Ala Gly Leu Asp Glu Thr
980 985 990
Ile Ala Lys Leu Thr Lys Glu Lys Lys Ala Leu Gln Glu Ala His Gln
995 1000 1005
Gln Thr Leu Asp Asp Leu Gln Ala Glu Glu Asp Lys Val Asn Thr
1010 1015 1020
Leu Thr Lys Ala Lys Thr Lys Leu Glu Gln Gln Val Asp Asp Leu
1025 1030 1035
Glu Gly Ser Leu Glu Gln Glu Lys Lys Leu Arg Met Asp Leu Glu
1040 1045 1050
Arg Ala Lys Arg Lys Leu Glu Gly Asp Leu Lys Leu Ala Gln Glu
1055 1060 1065
Ser Thr Met Asp Ile Glu Asn Asp Lys Gln Gln Leu Asp Glu Lys
1070 1075 1080
Leu Lys Lys Lys Glu Phe Glu Met Ser Asn Leu Gln Ser Lys Ile
1085 1090 1095
Glu Asp Glu Gln Ala Leu Gly Met Gln Leu Gln Lys Lys Ile Lys
1100 1105 1110
Glu Leu Gln Ala Arg Ile Glu Glu Leu Glu Glu Glu Ile Glu Ala
1115 1120 1125
Glu Arg Ala Ser Arg Ala Lys Ala Glu Lys Gln Arg Ser Asp Leu
1130 1135 1140
Ser Arg Glu Leu Glu Glu Ile Ser Glu Arg Leu Glu Glu Ala Gly
1145 1150 1155
Gly Ala Thr Ser Ala Gln Ile Glu Met Asn Lys Lys Arg Glu Ala
1160 1165 1170
Glu Phe Gln Lys Met Arg Arg Asp Leu Glu Glu Ala Thr Leu Gln
1175 1180 1185
His Glu Ala Thr Ala Ala Ala Leu Arg Lys Lys His Ala Asp Ser
1190 1195 1200
Val Ala Glu Leu Gly Glu Gln Leu Asp Asn Leu Gln Arg Val Lys
1205 1210 1215
Gln Lys Leu Glu Lys Glu Lys Ser Glu Met Lys Met Glu Ile Asp
1220 1225 1230
Asp Leu Ala Ser Asn Met Glu Thr Val Ser Lys Ala Lys Gly Asn
1235 1240 1245
Leu Glu Lys Met Cys Arg Thr Leu Glu Asp Gln Leu Ser Glu Leu
1250 1255 1260
Lys Thr Lys Glu Asp Glu Gln Gln Arg Leu Ile Asn Asp Leu Thr
1265 1270 1275
Thr Gln Arg Ala Arg Leu Gln Thr Glu Ser Gly Glu Phe Ser Arg
1280 1285 1290
Gln Leu Asp Glu Lys Asp Ala Leu Val Ser Gln Leu Ser Arg Gly
1295 1300 1305
Lys Gln Ala Phe Thr Gln Gln Ile Glu Glu Leu Lys Arg Gln Leu
1310 1315 1320
Glu Glu Glu Ile Lys Ala Lys Ser Ala Leu Ala His Ala Leu Gln
1325 1330 1335
Ser Ala Arg His Asp Cys Asp Leu Leu Arg Glu Gln Tyr Glu Glu
1340 1345 1350
Glu Gln Glu Gly Lys Ala Glu Leu Gln Arg Ala Met Ser Lys Ala
1355 1360 1365
Asn Ser Glu Val Ala Gln Trp Arg Thr Lys Tyr Glu Thr Asp Ala
1370 1375 1380
Ile Gln Arg Thr Glu Glu Leu Glu Glu Ala Lys Lys Lys Leu Ala
1385 1390 1395
Gln Arg Leu Gln Asp Ala Glu Glu His Val Glu Ala Val Asn Ala
1400 1405 1410
Lys Cys Ala Ser Leu Glu Lys Thr Lys Gln Arg Leu Gln Asn Glu
1415 1420 1425
Val Glu Asp Leu Met Ile Asp Val Glu Arg Thr Asn Ala Ala Cys
1430 1435 1440
Ala Ala Leu Asp Lys Lys Gln Arg Asn Phe Asp Lys Ile Leu Ser
1445 1450 1455
Glu Trp Lys Gln Lys Tyr Glu Glu Thr His Ala Glu Leu Glu Ala
1460 1465 1470
Ser Gln Lys Glu Ser Arg Ser Leu Ser Thr Glu Leu Phe Lys Ile
1475 1480 1485
Lys Asn Ala Tyr Glu Glu Ser Leu Asp Gln Leu Glu Thr Leu Lys
1490 1495 1500
Arg Glu Asn Lys Asn Leu Gln Gln Glu Ile Ser Asp Leu Thr Glu
1505 1510 1515
Gln Ile Ala Glu Gly Gly Lys Arg Ile His Glu Leu Glu Lys Val
1520 1525 1530
Lys Lys Gln Val Glu Gln Glu Lys Ser Glu Ile Gln Ala Ala Leu
1535 1540 1545
Glu Glu Ala Glu Ala Ser Leu Glu His Glu Glu Gly Lys Ile Leu
1550 1555 1560
Arg Ile Gln Leu Glu Leu Asn Gln Val Lys Ser Glu Ile Asp Arg
1565 1570 1575
Lys Ile Ala Glu Lys Asp Glu Glu Ile Asp Gln Leu Lys Arg Asn
1580 1585 1590
His Ile Arg Val Val Glu Ser Met Gln Ser Thr Leu Asp Ala Glu
1595 1600 1605
Ile Arg Ser Arg Asn Asp Ala Ile Arg Leu Lys Lys Lys Met Glu
1610 1615 1620
Gly Asp Leu Asn Glu Met Glu Ile Gln Leu Asn His Ala Asn Arg
1625 1630 1635
Met Ala Ala Glu Ala Leu Lys Asn Tyr Arg Asn Thr Gln Ala Ile
1640 1645 1650
Leu Lys Asp Thr Gln Leu His Leu Asp Asp Ala Leu Arg Gly Gln
1655 1660 1665
Glu Asp Leu Lys Glu Gln Leu Ala Met Val Glu Arg Arg Ala Asn
1670 1675 1680
Leu Leu Gln Ala Glu Ile Glu Glu Leu Arg Ala Thr Leu Glu Gln
1685 1690 1695
Thr Glu Arg Ser Arg Lys Ile Ala Glu Gln Glu Leu Leu Asp Ala
1700 1705 1710
Ser Glu Arg Val Gln Leu Leu His Thr Gln Asn Thr Ser Leu Ile
1715 1720 1725
Asn Thr Lys Lys Lys Leu Glu Thr Asp Ile Thr Gln Ile Gln Gly
1730 1735 1740
Glu Met Glu Asp Ile Ile Gln Glu Ala Arg Asn Ala Glu Glu Lys
1745 1750 1755
Ala Lys Lys Ala Ile Thr Asp Ala Ala Met Met Ala Glu Glu Leu
1760 1765 1770
Lys Lys Glu Gln Asp Thr Ser Ala His Leu Glu Arg Met Lys Lys
1775 1780 1785
Asn Leu Glu Gln Thr Val Lys Asp Leu Gln His Arg Leu Asp Glu
1790 1795 1800
Ala Glu Gln Leu Ala Leu Lys Gly Gly Lys Lys Gln Ile Gln Lys
1805 1810 1815
Leu Glu Ala Arg Val Arg Glu Leu Glu Ala Glu Val Glu Ser Glu
1820 1825 1830
Gln Lys Arg Asn Val Glu Thr Val Lys Gly Leu Arg Lys His Glu
1835 1840 1845
Arg Arg Val Lys Glu Leu Thr Tyr Gln Thr Glu Glu Asp Arg Lys
1850 1855 1860
Asn Ile Leu Arg Leu Gln Asp Leu Val Asp Lys Leu Gln Ala Lys
1865 1870 1875
Val Lys Ser Tyr Lys Arg Gln Ala Glu Glu Ala Glu Glu Gln Ser
1880 1885 1890
Asn Val Asn Leu Ser Lys Phe Arg Lys Leu Gln His Glu Leu Glu
1895 1900 1905
Glu Ala Glu Glu Arg Ala Asp Ile Ala Glu Ser Gln Val Asn Lys
1910 1915 1920
Leu Arg Val Lys Ser Arg Glu Val His Thr Lys Ile Ile Ser Glu
1925 1930 1935
Glu
<210> 9
<211> 1939
<212> PRT
<213> 山羊
<400> 9
Met Ser Ser Asp Gln Glu Met Ala Ile Phe Gly Glu Ala Ala Pro Tyr
1 5 10 15
Leu Arg Lys Ser Glu Lys Glu Arg Ile Glu Ala Gln Asn Lys Pro Phe
20 25 30
Asp Ala Lys Thr Ser Val Phe Val Ala Asp Pro Lys Glu Ser Phe Val
35 40 45
Lys Ala Thr Val Gln Ser Arg Glu Gly Gly Lys Val Thr Ala Lys Thr
50 55 60
Glu Ala Gly Ala Thr Val Thr Val Lys Glu Asp Gln Val Phe Pro Met
65 70 75 80
Asn Pro Pro Lys Phe Asp Lys Ile Glu Asp Met Ala Met Met Thr His
85 90 95
Leu His Glu Pro Gly Val Leu Tyr Asn Leu Lys Glu Arg Tyr Ala Ala
100 105 110
Trp Met Ile Tyr Thr Tyr Ser Gly Leu Phe Cys Val Thr Val Asn Pro
115 120 125
Tyr Lys Trp Leu Pro Val Tyr Asn Ala Glu Val Val Ala Ala Tyr Arg
130 135 140
Gly Lys Lys Arg Gln Glu Ala Pro Pro His Ile Phe Ser Ile Ser Asp
145 150 155 160
Asn Ala Tyr Gln Phe Met Leu Thr Asp Arg Glu Asn Gln Ser Ile Leu
165 170 175
Ile Thr Gly Glu Ser Gly Ala Gly Lys Thr Val Asn Thr Lys Arg Val
180 185 190
Ile Gln Tyr Phe Ala Thr Ile Ala Val Thr Gly Glu Lys Lys Lys Glu
195 200 205
Glu Pro Thr Ser Gly Lys Met Gln Gly Thr Leu Glu Asp Gln Ile Ile
210 215 220
Ser Ala Asn Pro Leu Leu Glu Ala Phe Gly Asn Ala Lys Thr Val Arg
225 230 235 240
Asn Asp Asn Ser Ser Arg Phe Gly Lys Phe Ile Arg Ile His Phe Gly
245 250 255
Thr Thr Gly Lys Leu Ala Ser Ala Asp Ile Glu Thr Tyr Leu Leu Glu
260 265 270
Lys Ser Arg Val Thr Phe Gln Leu Lys Ala Glu Arg Ser Tyr His Ile
275 280 285
Phe Tyr Gln Ile Met Ser Asn Lys Lys Pro Glu Leu Ile Glu Met Leu
290 295 300
Leu Ile Thr Thr Asn Pro Tyr Asp Tyr Ala Phe Val Ser Gln Gly Glu
305 310 315 320
Ile Thr Val Pro Ser Ile Asp Asp Gln Glu Glu Leu Met Ala Thr Asp
325 330 335
Ser Ala Ile Glu Ile Leu Gly Phe Thr Ser Asp Glu Arg Val Ser Ile
340 345 350
Tyr Lys Leu Thr Gly Ala Val Met His Tyr Gly Asn Leu Lys Phe Lys
355 360 365
Gln Lys Gln Arg Glu Glu Gln Ala Glu Pro Asp Gly Thr Glu Val Ala
370 375 380
Asp Lys Ala Ala Tyr Leu Gln Gly Leu Asn Ser Ala Asp Leu Leu Lys
385 390 395 400
Ala Leu Cys Tyr Pro Arg Val Lys Val Gly Asn Glu Phe Val Thr Lys
405 410 415
Gly Gln Thr Val Glu Gln Val Tyr Asn Ala Val Gly Ala Leu Ala Lys
420 425 430
Ala Val Tyr Asp Lys Met Phe Leu Trp Met Val Thr Arg Ile Asn Gln
435 440 445
Gln Leu Asp Thr Lys Gln Pro Arg Gln Tyr Phe Ile Gly Val Leu Asp
450 455 460
Ile Ala Gly Phe Glu Ile Phe Asp Phe Asn Ser Leu Glu Gln Leu Cys
465 470 475 480
Ile Asn Phe Thr Asn Glu Lys Leu Gln Gln Phe Phe Asn His His Met
485 490 495
Phe Val Leu Glu Gln Glu Glu Tyr Lys Lys Glu Gly Ile Glu Trp Glu
500 505 510
Phe Ile Asp Phe Gly Met Asp Leu Ala Ala Cys Ile Glu Leu Ile Glu
515 520 525
Lys Pro Met Gly Ile Phe Ser Ile Leu Glu Glu Glu Cys Met Phe Pro
530 535 540
Lys Ala Thr Asp Thr Ser Phe Lys Asn Lys Leu Tyr Glu Gln His Leu
545 550 555 560
Gly Lys Ser Asn Asn Phe Gln Lys Pro Lys Pro Ala Lys Gly Lys Ala
565 570 575
Glu Ala His Phe Ser Leu Ile His Tyr Ala Gly Thr Val Asp Tyr Asn
580 585 590
Ile Thr Gly Trp Leu Asp Lys Asn Lys Asp Pro Leu Asn Glu Thr Val
595 600 605
Val Gly Leu Tyr Gln Lys Ser Ser Val Lys Thr Leu Ala Phe Leu Phe
610 615 620
Ser Gly Ala Ala Ser Ala Glu Ala Glu Gly Gly Gly Gly Lys Lys Gly
625 630 635 640
Gly Lys Lys Lys Gly Ser Ser Phe Gln Thr Val Ser Ala Leu Phe Arg
645 650 655
Glu Asn Leu Asn Lys Leu Met Thr Asn Leu Arg Ser Thr His Pro His
660 665 670
Phe Val Arg Cys Ile Ile Pro Asn Glu Thr Lys Thr Pro Gly Ala Met
675 680 685
Glu His Glu Leu Val Leu His Gln Leu Arg Cys Asn Gly Val Leu Glu
690 695 700
Gly Ile Arg Ile Cys Arg Lys Gly Phe Pro Ser Arg Ile Leu Tyr Ala
705 710 715 720
Asp Phe Lys Gln Arg Tyr Lys Val Leu Asn Ala Ser Ala Ile Pro Glu
725 730 735
Gly Gln Phe Ile Asp Ser Lys Lys Ala Ser Glu Lys Leu Leu Gly Ser
740 745 750
Ile Asp Val Asp His Thr Gln Tyr Lys Phe Gly His Thr Lys Val Phe
755 760 765
Phe Lys Ala Gly Leu Leu Gly Leu Leu Glu Glu Met Arg Asp Glu Lys
770 775 780
Leu Ala Gln Leu Ile Thr Arg Thr Gln Ala Arg Cys Arg Gly Phe Leu
785 790 795 800
Ala Arg Val Glu Tyr Gln Arg Met Val Glu Arg Arg Glu Ser Ile Phe
805 810 815
Cys Ile Gln Tyr Asn Val Arg Ala Phe Met Asn Val Lys His Trp Pro
820 825 830
Trp Met Lys Leu Tyr Phe Lys Ile Lys Pro Leu Leu Lys Ser Ala Glu
835 840 845
Thr Glu Lys Glu Met Ala Asn Met Lys Glu Glu Phe Glu Lys Thr Lys
850 855 860
Glu Glu Leu Ala Lys Ser Glu Ala Lys Arg Lys Glu Leu Glu Glu Lys
865 870 875 880
Met Val Thr Leu Thr Gln Glu Lys Asn Asp Leu Gln Leu Gln Val Gln
885 890 895
Ser Glu Ala Asp Ala Leu Ala Asp Ala Glu Glu Arg Cys Asp Gln Leu
900 905 910
Ile Lys Thr Lys Ile Gln Leu Glu Ala Lys Ile Lys Glu Val Thr Glu
915 920 925
Arg Ala Glu Asp Glu Glu Glu Ile Asn Ala Glu Leu Thr Ala Lys Lys
930 935 940
Arg Lys Leu Glu Asp Glu Cys Ser Glu Leu Lys Lys Asp Ile Asp Asp
945 950 955 960
Leu Glu Leu Thr Leu Ala Lys Val Glu Lys Glu Lys His Ala Thr Glu
965 970 975
Asn Lys Val Lys Asn Leu Thr Glu Glu Met Ala Gly Leu Asp Glu Thr
980 985 990
Ile Ala Lys Leu Thr Lys Glu Lys Lys Ala Leu Gln Glu Ala His Gln
995 1000 1005
Gln Thr Leu Asp Asp Leu Gln Ala Glu Glu Asp Lys Val Asn Thr
1010 1015 1020
Leu Thr Lys Ala Lys Thr Lys Leu Glu Gln Gln Val Asp Asp Leu
1025 1030 1035
Glu Gly Ser Leu Glu Gln Glu Lys Lys Leu Arg Met Asp Leu Glu
1040 1045 1050
Arg Ala Lys Arg Lys Leu Glu Gly Asp Leu Lys Leu Ala Gln Glu
1055 1060 1065
Ser Thr Met Asp Ile Glu Asn Asp Lys Gln Gln Leu Asp Glu Lys
1070 1075 1080
Leu Lys Lys Lys Glu Phe Glu Met Ser Asn Leu Gln Ser Lys Ile
1085 1090 1095
Glu Asp Glu Gln Ala Leu Gly Met Gln Leu Gln Lys Lys Ile Lys
1100 1105 1110
Glu Leu Gln Ala Arg Ile Glu Glu Leu Glu Glu Glu Ile Glu Ala
1115 1120 1125
Glu Arg Ala Ser Arg Ala Lys Ala Glu Lys Gln Arg Ser Asp Leu
1130 1135 1140
Ser Arg Glu Leu Glu Glu Ile Ser Glu Arg Leu Glu Glu Ala Gly
1145 1150 1155
Gly Ala Thr Ser Ala Gln Ile Glu Met Asn Lys Lys Arg Glu Ala
1160 1165 1170
Glu Phe Gln Lys Met Arg Arg Asp Leu Glu Glu Ala Thr Leu Gln
1175 1180 1185
His Glu Ala Thr Ala Ala Ala Leu Arg Lys Lys His Ala Asp Ser
1190 1195 1200
Val Ala Glu Leu Gly Glu Gln Leu Asp Asn Leu Gln Arg Val Lys
1205 1210 1215
Gln Lys Leu Glu Lys Glu Lys Ser Glu Met Lys Met Glu Ile Asp
1220 1225 1230
Asp Leu Ala Ser Asn Met Glu Thr Val Ser Lys Ala Lys Gly Asn
1235 1240 1245
Leu Glu Lys Met Cys Arg Thr Leu Glu Asp Gln Leu Ser Glu Leu
1250 1255 1260
Lys Thr Lys Glu Asp Glu Gln Gln Arg Leu Ile Asn Asp Leu Thr
1265 1270 1275
Thr Gln Arg Ala Arg Leu Gln Thr Glu Ser Gly Glu Phe Ser Arg
1280 1285 1290
Gln Leu Asp Glu Lys Asp Ala Leu Val Ser Gln Leu Ser Arg Gly
1295 1300 1305
Lys Gln Ala Phe Thr Gln Gln Ile Glu Glu Leu Lys Arg Gln Leu
1310 1315 1320
Glu Glu Glu Ile Lys Ala Lys Ser Ala Leu Ala His Ala Leu Gln
1325 1330 1335
Ser Ala Arg His Asp Cys Asp Leu Leu Arg Glu Gln Tyr Glu Glu
1340 1345 1350
Glu Gln Glu Gly Lys Ala Glu Leu Gln Arg Ala Met Ser Lys Ala
1355 1360 1365
Asn Ser Glu Val Ala Gln Trp Arg Thr Lys Tyr Glu Thr Asp Ala
1370 1375 1380
Ile Gln Arg Thr Glu Glu Leu Glu Glu Ala Lys Lys Lys Leu Ala
1385 1390 1395
Gln Arg Leu Gln Glu Ala Glu Glu His Val Glu Ala Val Asn Ala
1400 1405 1410
Lys Cys Ala Ser Leu Glu Lys Thr Lys Gln Arg Leu Gln Asn Glu
1415 1420 1425
Val Glu Asp Leu Met Ile Asp Val Glu Arg Thr Asn Ala Ala Cys
1430 1435 1440
Ala Ala Leu Asp Lys Lys Gln Arg Asn Phe Asp Lys Ile Leu Ser
1445 1450 1455
Glu Trp Lys Gln Lys Tyr Glu Glu Thr His Ala Glu Leu Glu Ala
1460 1465 1470
Ser Gln Lys Glu Ser Arg Ser Leu Ser Thr Glu Leu Phe Lys Ile
1475 1480 1485
Lys Asn Ala Tyr Glu Glu Ser Leu Asp Gln Leu Glu Thr Leu Lys
1490 1495 1500
Arg Glu Asn Lys Asn Leu Gln Gln Glu Ile Ser Asp Leu Thr Glu
1505 1510 1515
Gln Ile Ala Glu Gly Gly Lys Arg Ile His Glu Leu Glu Lys Val
1520 1525 1530
Lys Lys Gln Val Glu Gln Glu Lys Ser Glu Ile Gln Ala Ala Leu
1535 1540 1545
Glu Glu Ala Glu Ala Ser Leu Glu His Glu Glu Gly Lys Ile Leu
1550 1555 1560
Arg Ile Gln Leu Glu Leu Asn Gln Val Lys Ser Glu Ile Asp Arg
1565 1570 1575
Lys Ile Ala Glu Lys Asp Glu Glu Ile Asp Gln Leu Lys Arg Asn
1580 1585 1590
His Ile Arg Val Val Glu Ser Met Gln Ser Thr Leu Asp Ala Glu
1595 1600 1605
Ile Arg Ser Arg Asn Asp Ala Ile Arg Leu Lys Lys Lys Met Glu
1610 1615 1620
Gly Asp Leu Asn Glu Met Glu Ile Gln Leu Asn His Ala Asn Arg
1625 1630 1635
Met Ala Ala Glu Ala Leu Lys Asn Tyr Arg Asn Thr Gln Ala Ile
1640 1645 1650
Leu Lys Asp Thr Gln Leu His Leu Asp Asp Ala Leu Arg Gly Gln
1655 1660 1665
Glu Asp Leu Lys Glu Gln Leu Ala Met Val Glu Arg Arg Ala Asn
1670 1675 1680
Leu Leu Gln Ala Glu Ile Glu Glu Leu Arg Ala Thr Leu Glu Gln
1685 1690 1695
Thr Glu Arg Ser Arg Lys Ile Ala Glu Gln Glu Leu Leu Asp Ala
1700 1705 1710
Ser Glu Arg Val Gln Leu Leu His Thr Gln Asn Thr Ser Leu Ile
1715 1720 1725
Asn Thr Lys Lys Lys Leu Glu Thr Asp Ile Thr Gln Ile Gln Gly
1730 1735 1740
Glu Met Glu Asp Ile Ile Gln Glu Ala Arg Asn Ala Glu Glu Lys
1745 1750 1755
Ala Lys Lys Ala Ile Thr Asp Ala Ala Met Met Ala Glu Glu Leu
1760 1765 1770
Lys Lys Glu Gln Asp Thr Ser Ala His Leu Glu Arg Met Lys Lys
1775 1780 1785
Asn Leu Glu Gln Thr Val Lys Asp Leu Gln His Arg Leu Asp Glu
1790 1795 1800
Ala Glu Gln Leu Ala Leu Lys Gly Gly Lys Lys Gln Ile Gln Lys
1805 1810 1815
Leu Glu Ala Arg Val Arg Glu Leu Glu Ala Glu Val Glu Ser Glu
1820 1825 1830
Gln Lys Arg Asn Val Glu Thr Val Lys Gly Leu Arg Lys His Glu
1835 1840 1845
Arg Arg Val Lys Glu Leu Thr Tyr Gln Thr Glu Glu Asp Arg Lys
1850 1855 1860
Asn Ile Leu Arg Leu Gln Asp Leu Val Asp Lys Leu Gln Ala Lys
1865 1870 1875
Val Lys Ser Tyr Lys Arg Gln Ala Glu Glu Ala Glu Glu Gln Ser
1880 1885 1890
Asn Val Asn Leu Ser Lys Phe Arg Lys Leu Gln His Glu Leu Glu
1895 1900 1905
Glu Ala Glu Glu Arg Ala Asp Ile Ala Glu Ser Gln Val Asn Lys
1910 1915 1920
Leu Arg Val Lys Ser Arg Glu Val His Thr Lys Ile Ile Ser Glu
1925 1930 1935
Glu
<210> 10
<211> 1940
<212> PRT
<213> 绿头鸭
<400> 10
Met Ser Ser Asp Ser Glu Met Ala Ile Phe Gly Glu Ala Ala Pro Tyr
1 5 10 15
Leu Arg Lys Ser Glu Lys Glu Arg Ile Glu Ala Gln Asn Lys Pro Phe
20 25 30
Asp Ala Lys Ser Ser Val Phe Val Val His Pro Lys Glu Ser Phe Val
35 40 45
Lys Gly Thr Ile Gln Ser Lys Glu Ser Gly Lys Val Thr Val Lys Thr
50 55 60
Glu Gly Gly Glu Thr Leu Thr Val Lys Glu Asp Gln Ile Phe Ser Met
65 70 75 80
Asn Pro Pro Lys Tyr Asp Lys Ile Glu Asp Met Ala Met Met Thr His
85 90 95
Leu His Glu Pro Ala Val Leu Tyr Asn Leu Lys Glu Arg Tyr Ala Ala
100 105 110
Trp Met Ile Tyr Thr Tyr Ser Gly Leu Phe Cys Val Thr Val Asn Pro
115 120 125
Tyr Lys Trp Leu Pro Val Tyr Asn Pro Glu Val Val Leu Ala Tyr Arg
130 135 140
Gly Lys Lys Arg Gln Glu Ala Pro Pro His Ile Phe Ser Ile Ser Asp
145 150 155 160
Asn Ala Tyr Gln Phe Met Leu Thr Asp Arg Glu Asn Gln Ser Ile Leu
165 170 175
Ile Thr Gly Glu Ser Gly Ala Gly Lys Thr Val Asn Thr Lys Arg Val
180 185 190
Ile Gln Tyr Phe Ala Thr Ile Ala Ala Ser Gly Glu Lys Lys Lys Glu
195 200 205
Glu Gln Pro Gly Lys Met Gln Gly Thr Leu Glu Asp Gln Ile Ile Ser
210 215 220
Ala Asn Pro Leu Leu Glu Ala Phe Gly Asn Ala Lys Thr Val Arg Asn
225 230 235 240
Asp Asn Ser Ser Arg Phe Gly Lys Phe Ile Arg Ile His Phe Gly Ala
245 250 255
Thr Gly Lys Leu Ala Ser Ala Asp Ile Glu Thr Tyr Leu Leu Glu Lys
260 265 270
Ser Arg Val Thr Phe Gln Leu Lys Ala Glu Arg Ser Tyr His Ile Phe
275 280 285
Tyr Gln Ile Met Ser Asn Lys Lys Pro Glu Leu Ile Asp Met Leu Leu
290 295 300
Ile Thr Thr Asn Pro Tyr Asp Tyr Gln Phe Val Ser Gln Gly Glu Ile
305 310 315 320
Thr Val Pro Ser Ile Asn Asp Gln Glu Glu Leu Ile Ala Thr Asp Ser
325 330 335
Ala Ile Asp Ile Leu Gly Phe Thr Ala Asp Glu Lys Thr Ala Ile Tyr
340 345 350
Lys Leu Thr Gly Ala Val Met His Tyr Gly Asn Leu Lys Phe Lys Gln
355 360 365
Lys Gln Arg Glu Glu Gln Ala Glu Pro Asp Gly Thr Glu Val Ala Asp
370 375 380
Lys Ala Ala Tyr Leu Met Gly Leu Asn Ser Ala Asp Leu Leu Lys Ala
385 390 395 400
Leu Cys Tyr Pro Arg Val Lys Val Gly Asn Glu Tyr Val Thr Lys Gly
405 410 415
Gln Thr Val Gln Gln Val Asn Asn Ala Val Gly Ala Leu Ala Lys Ala
420 425 430
Val Tyr Glu Lys Met Phe Leu Trp Met Val Val Arg Ile Asn Gln Gln
435 440 445
Leu Asp Thr Lys Gln Pro Arg Gln Tyr Phe Ile Gly Val Leu Asp Ile
450 455 460
Ala Gly Phe Glu Ile Phe Asp Phe Asn Ser Leu Glu Gln Leu Cys Ile
465 470 475 480
Asn Phe Thr Asn Glu Lys Leu Gln Gln Phe Phe Asn His His Met Phe
485 490 495
Val Leu Glu Gln Glu Glu Tyr Lys Lys Glu Gly Ile Glu Trp Glu Phe
500 505 510
Ile Asp Phe Gly Met Asp Leu Ala Ala Cys Ile Glu Leu Ile Glu Lys
515 520 525
Pro Met Gly Ile Phe Ser Ile Leu Glu Glu Glu Cys Met Phe Pro Lys
530 535 540
Ala Thr Asp Thr Ser Phe Lys Asn Lys Leu Tyr Asp Gln His Leu Gly
545 550 555 560
Lys Ser Asn Asn Phe Gln Lys Pro Lys Pro Gly Lys Gly Lys Ala Glu
565 570 575
Ala His Phe Ser Leu Val His Tyr Ala Gly Thr Val Asp Tyr Asn Ile
580 585 590
Ser Gly Trp Leu Glu Lys Asn Lys Asp Pro Leu Asn Glu Thr Val Ile
595 600 605
Gly Leu Tyr Gln Lys Ser Ser Val Lys Thr Leu Ala Leu Leu Phe Ala
610 615 620
Ser Ala Gly Gly Glu Ala Glu Ala Ser Gly Gly Gly Gly Lys Lys Gly
625 630 635 640
Gly Lys Lys Lys Gly Ser Ser Phe Gln Thr Val Ser Ala Leu Phe Arg
645 650 655
Glu Asn Leu Asn Lys Leu Met Thr Asn Leu Arg Ser Thr His Pro His
660 665 670
Phe Val Arg Cys Ile Ile Pro Asn Glu Thr Lys Thr Pro Gly Ala Met
675 680 685
Glu His Glu Leu Val Leu His Gln Leu Arg Cys Asn Gly Val Leu Glu
690 695 700
Gly Ile Arg Ile Cys Arg Lys Gly Phe Pro Ser Arg Val Leu Tyr Ala
705 710 715 720
Asp Phe Lys Gln Arg Tyr Lys Val Leu Asn Ala Ser Ala Ile Pro Glu
725 730 735
Gly Gln Phe Ile Asp Ser Lys Lys Ala Ser Glu Lys Leu Leu Gly Ser
740 745 750
Ile Asp Val Asp His Thr Gln Tyr Lys Phe Gly His Thr Lys Val Phe
755 760 765
Phe Lys Ala Gly Leu Leu Gly Leu Leu Glu Glu Met Arg Asp Glu Lys
770 775 780
Leu Ala Gln Leu Ile Thr Arg Thr Gln Ala Arg Cys Arg Gly Phe Leu
785 790 795 800
Met Arg Val Glu Tyr Gln Arg Met Val Glu Arg Arg Glu Ser Ile Phe
805 810 815
Cys Ile Gln Tyr Asn Val Arg Ala Phe Met Asn Val Lys His Trp Pro
820 825 830
Trp Met Lys Leu Phe Phe Lys Ile Lys Pro Leu Leu Lys Ser Ala Glu
835 840 845
Ser Glu Lys Glu Met Ala Asn Met Lys Gly Glu Phe Glu Lys Thr Lys
850 855 860
Glu Glu Leu Ala Lys Ser Glu Ala Lys Arg Lys Glu Leu Glu Glu Lys
865 870 875 880
Met Val Ser Leu Leu Gln Glu Lys Asn Asp Leu Gln Leu Gln Val Gln
885 890 895
Ala Glu Ala Asp Ser Leu Ala Asp Ala Glu Glu Arg Cys Asp Gln Leu
900 905 910
Ile Lys Thr Lys Ile Gln Leu Glu Ala Lys Ile Lys Glu Val Thr Glu
915 920 925
Arg Ala Glu Asp Glu Glu Glu Ile Asn Ala Glu Leu Thr Ala Lys Lys
930 935 940
Arg Lys Leu Glu Asp Glu Cys Ser Glu Leu Lys Lys Asp Ile Asp Asp
945 950 955 960
Leu Glu Leu Thr Leu Ala Lys Val Glu Lys Glu Lys His Ala Thr Glu
965 970 975
Asn Lys Val Lys Asn Leu Thr Glu Glu Met Ala Ser Leu Asp Glu Thr
980 985 990
Ile Ala Lys Leu Thr Lys Glu Lys Lys Ala Leu Gln Glu Ala His Gln
995 1000 1005
Gln Thr Leu Asp Asp Leu Gln Ala Glu Glu Asp Lys Val Asn Thr
1010 1015 1020
Leu Thr Lys Ala Lys Thr Lys Leu Glu Gln Gln Val Asp Asp Leu
1025 1030 1035
Glu Gly Ser Leu Glu Gln Glu Lys Lys Leu Arg Met Asp Leu Glu
1040 1045 1050
Arg Ala Lys Arg Lys Leu Glu Gly Asp Leu Lys Leu Ala His Asp
1055 1060 1065
Ser Ile Met Asp Leu Glu Asn Asp Lys Gln Gln Leu Asp Glu Lys
1070 1075 1080
Leu Lys Lys Lys Asp Phe Glu Ile Ser Gln Ile Gln Ser Lys Ile
1085 1090 1095
Glu Asp Glu Gln Ala Leu Gly Met Gln Leu Gln Lys Lys Ile Lys
1100 1105 1110
Glu Leu Gln Ala Arg Ile Glu Glu Leu Glu Glu Glu Ile Glu Ala
1115 1120 1125
Glu Arg Thr Ser Arg Ala Lys Ala Glu Lys His Arg Ala Asp Leu
1130 1135 1140
Ser Arg Glu Leu Glu Glu Ile Ser Glu Arg Leu Glu Glu Ala Gly
1145 1150 1155
Gly Ala Thr Ala Ala Gln Ile Glu Met Asn Lys Lys Arg Glu Ser
1160 1165 1170
Glu Phe Gln Lys Met Arg Arg Asp Leu Glu Glu Ala Thr Leu Gln
1175 1180 1185
His Glu Ala Thr Ala Ala Ala Leu Arg Lys Lys His Ala Asp Ser
1190 1195 1200
Thr Ala Glu Leu Gly Glu Gln Ile Asp Asn Leu Gln Arg Val Lys
1205 1210 1215
Gln Lys Leu Glu Lys Glu Lys Ser Glu Leu Lys Met Glu Ile Asp
1220 1225 1230
Asp Leu Ala Ser Asn Met Glu Ser Val Ser Lys Ala Lys Ala Asn
1235 1240 1245
Leu Glu Lys Met Cys Arg Thr Leu Glu Asp Gln Leu Ser Glu Val
1250 1255 1260
Lys Thr Lys Glu Glu Glu His Gln Arg Met Val Asn Asp Leu Asn
1265 1270 1275
Ala Gln Arg Ala Arg Leu Gln Thr Glu Ser Gly Glu Tyr Ser Arg
1280 1285 1290
Gln Val Glu Glu Lys Asp Ala Leu Ile Ser Gln Leu Ser Arg Gly
1295 1300 1305
Lys Gln Ala Phe Thr Gln Gln Ile Glu Glu Leu Lys Arg His Leu
1310 1315 1320
Glu Glu Glu Ile Lys Ala Lys Ser Ala Leu Ala His Ala Leu Gln
1325 1330 1335
Ser Ala Arg His Asp Cys Asp Leu Leu Arg Glu Gln Tyr Glu Glu
1340 1345 1350
Glu Gln Glu Ala Lys Gly Glu Leu Gln Arg Ala Leu Ser Lys Ala
1355 1360 1365
Asn Ser Glu Val Ala Gln Trp Arg Thr Lys Tyr Glu Thr Asp Ala
1370 1375 1380
Ile Gln Arg Thr Glu Glu Leu Glu Glu Ala Lys Lys Lys Leu Ala
1385 1390 1395
Gln Arg Leu Gln Asp Ala Glu Glu His Val Glu Ala Val Asn Ala
1400 1405 1410
Lys Cys Ala Ser Leu Glu Lys Thr Lys Gln Arg Leu Gln Asn Glu
1415 1420 1425
Val Glu Asp Leu Met Ile Asp Val Glu Arg Ser Asn Ala Ala Cys
1430 1435 1440
Ala Ala Leu Asp Lys Lys Gln Lys Asn Phe Asp Lys Ile Leu Ala
1445 1450 1455
Glu Trp Lys Gln Lys Tyr Glu Glu Thr Gln Ala Glu Leu Glu Ala
1460 1465 1470
Ser Gln Lys Glu Ser Arg Ser Leu Ser Thr Glu Leu Phe Lys Met
1475 1480 1485
Lys Asn Ala Tyr Glu Glu Ser Leu Asp His Leu Glu Thr Leu Lys
1490 1495 1500
Arg Glu Asn Lys Asn Leu Gln Gln Glu Ile Ser Asp Leu Thr Glu
1505 1510 1515
Gln Ile Ala Glu Gly Gly Lys Ala Ile His Glu Leu Glu Lys Val
1520 1525 1530
Lys Lys Gln Ile Glu Gln Glu Lys Ser Glu Ile Gln Ala Ala Leu
1535 1540 1545
Glu Glu Ala Glu Ala Ser Leu Glu His Glu Glu Gly Lys Ile Leu
1550 1555 1560
Arg Leu Gln Leu Glu Leu Asn Gln Val Lys Ser Glu Ile Asp Arg
1565 1570 1575
Lys Ile Ala Glu Lys Asp Glu Glu Ile Asp Gln Leu Lys Arg Asn
1580 1585 1590
His Leu Arg Ile Val Glu Ser Met Gln Ser Thr Leu Asp Ala Glu
1595 1600 1605
Ile Arg Ser Arg Asn Glu Ala Leu Arg Leu Lys Lys Lys Met Glu
1610 1615 1620
Gly Asp Leu Asn Glu Met Glu Ile Gln Leu Ser His Ala Asn Arg
1625 1630 1635
Gln Ala Ala Glu Ala Gln Lys Asn Leu Arg Asn Thr Gln Gly Val
1640 1645 1650
Leu Lys Asp Thr Gln Ile His Leu Asp Asp Ala Leu Arg Thr Gln
1655 1660 1665
Glu Asp Leu Lys Glu Gln Val Ala Met Val Glu Arg Arg Ala Asn
1670 1675 1680
Leu Leu Gln Ala Glu Ile Glu Glu Leu Arg Ala Ala Leu Glu Gln
1685 1690 1695
Thr Glu Arg Ser Arg Lys Val Ala Glu Gln Glu Leu Met Asp Ala
1700 1705 1710
Ser Glu Arg Val Gln Leu Leu His Thr Gln Asn Thr Ser Leu Ile
1715 1720 1725
Asn Thr Lys Lys Lys Leu Glu Thr Asp Ile Ala Gln Ile Gln Gly
1730 1735 1740
Glu Met Glu Asp Thr Ile Gln Glu Ala Arg Asn Ala Glu Glu Lys
1745 1750 1755
Ala Lys Lys Ala Ile Thr Asp Ala Ala Met Met Ala Glu Glu Leu
1760 1765 1770
Lys Lys Glu Gln Asp Thr Ser Ala His Leu Glu Arg Met Lys Lys
1775 1780 1785
Asn Leu Asp Gln Thr Val Lys Asp Leu Gln His Arg Leu Asp Glu
1790 1795 1800
Ala Glu Gln Leu Ala Leu Lys Gly Gly Lys Lys Gln Ile Gln Lys
1805 1810 1815
Leu Glu Ala Arg Val Arg Glu Leu Glu Gly Glu Val Asp Ala Glu
1820 1825 1830
Gln Lys Arg Ser Ala Glu Ala Val Lys Gly Val Arg Lys Tyr Glu
1835 1840 1845
Arg Arg Val Lys Glu Leu Thr Tyr Gln Ser Glu Glu Asp Arg Lys
1850 1855 1860
Asn Ile Leu Arg Leu Gln Asp Leu Val Asp Lys Leu Gln Met Lys
1865 1870 1875
Val Lys Ser Tyr Lys Arg Gln Ala Glu Glu Ala Glu Glu Leu Ser
1880 1885 1890
Asn Val Asn Leu Ser Lys Phe Arg Lys Ile Gln His Glu Leu Glu
1895 1900 1905
Glu Ala Glu Glu Arg Ala Asp Ile Ala Glu Ser Gln Val Asn Lys
1910 1915 1920
Leu Arg Val Lys Ser Arg Glu Phe His Ser Lys Lys Ile Glu Glu
1925 1930 1935
Glu Glu
1940
<210> 11
<211> 1939
<212> PRT
<213> 兔
<400> 11
Met Ser Ser Asp Ala Asp Met Ala Val Phe Gly Glu Ala Ala Pro Tyr
1 5 10 15
Leu Arg Lys Ser Glu Lys Glu Arg Ile Glu Ala Gln Asn Lys Pro Phe
20 25 30
Asp Ala Lys Asn Ser Val Phe Val Val Asp Pro Lys Glu Ser Tyr Val
35 40 45
Lys Ser Thr Val Gln Ser Arg Glu Gly Gly Lys Val Thr Val Lys Thr
50 55 60
Glu Ala Gly Ala Thr Val Thr Val Lys Glu Asp Gln Val Phe Ser Met
65 70 75 80
Asn Pro Pro Lys Tyr Asp Lys Ile Glu Asp Met Ala Met Met Thr His
85 90 95
Leu His Glu Pro Ala Val Leu Tyr Asn Leu Lys Glu Arg Tyr Ala Ala
100 105 110
Trp Met Ile Tyr Thr Tyr Ser Gly Leu Phe Cys Val Thr Val Asn Pro
115 120 125
Tyr Lys Trp Leu Pro Val Tyr Asn Pro Glu Val Val Thr Ala Tyr Arg
130 135 140
Gly Lys Lys Arg Gln Glu Ala Pro Pro His Ile Phe Ser Ile Ser Asp
145 150 155 160
Asn Ala Tyr Gln Phe Met Leu Thr Asp Arg Glu Asn Gln Ser Ile Leu
165 170 175
Ile Thr Gly Glu Ser Gly Ala Gly Lys Thr Val Asn Thr Lys Arg Val
180 185 190
Ile Gln Tyr Phe Ala Thr Ile Ala Ile Thr Gly Asp Lys Lys Lys Glu
195 200 205
Glu Pro Thr Pro Gly Lys Met Gln Gly Thr Leu Glu Asp Gln Ile Ile
210 215 220
Ser Ala Asn Pro Leu Leu Glu Ala Phe Gly Asn Ala Lys Thr Val Arg
225 230 235 240
Asn Asp Asn Ser Ser Arg Phe Gly Lys Phe Ile Arg Ile His Phe Gly
245 250 255
Ala Thr Gly Lys Leu Ala Ser Ala Asp Ile Glu Thr Tyr Leu Leu Glu
260 265 270
Lys Ser Arg Val Thr Phe Gln Leu Lys Ala Glu Arg Ser Tyr His Ile
275 280 285
Phe Tyr Gln Ile Met Ser Asn Lys Lys Pro Glu Leu Ile Glu Met Leu
290 295 300
Leu Ile Thr Thr Asn Pro Tyr Asp Phe Ala Phe Val Ser Gln Gly Glu
305 310 315 320
Ile Thr Val Pro Ser Ile Asp Asp Gln Glu Glu Leu Met Ala Thr Asp
325 330 335
Ser Ala Val Asp Ile Leu Gly Phe Thr Ser Asp Glu Lys Val Ala Ile
340 345 350
Tyr Lys Leu Thr Gly Ala Val Met His Tyr Gly Asn Leu Lys Phe Lys
355 360 365
Gln Lys Gln Arg Glu Glu Gln Ala Glu Pro Asp Gly Thr Glu Val Ala
370 375 380
Asp Lys Ala Ala Tyr Leu Thr Ser Leu Asn Ser Ala Asp Leu Leu Lys
385 390 395 400
Ala Leu Cys Tyr Pro Arg Val Lys Val Gly Asn Glu Tyr Val Thr Lys
405 410 415
Gly Gln Thr Val Gln Gln Val Tyr Asn Ala Val Gly Ala Leu Ala Lys
420 425 430
Ala Ile Tyr Glu Lys Met Phe Leu Trp Met Val Thr Arg Ile Asn Gln
435 440 445
Gln Leu Asp Thr Lys Gln Pro Arg Gln Tyr Phe Ile Gly Val Leu Asp
450 455 460
Ile Ala Gly Phe Glu Ile Phe Asp Phe Asn Ser Leu Glu Gln Leu Cys
465 470 475 480
Ile Asn Phe Thr Asn Glu Lys Leu Gln Gln Phe Phe Asn His His Met
485 490 495
Phe Val Leu Glu Gln Glu Glu Tyr Lys Lys Glu Gly Ile Glu Trp Glu
500 505 510
Phe Ile Asp Phe Gly Met Asp Leu Ala Ala Cys Ile Glu Leu Ile Glu
515 520 525
Lys Pro Met Gly Ile Phe Ser Ile Leu Glu Glu Glu Cys Met Phe Pro
530 535 540
Lys Ala Thr Asp Thr Ser Phe Lys Asn Lys Leu Tyr Glu Gln His Leu
545 550 555 560
Gly Lys Ser Asn Asn Phe Gln Lys Pro Lys Pro Ala Lys Gly Lys Ala
565 570 575
Glu Ala His Phe Ser Leu Val His Tyr Ala Gly Thr Val Asp Tyr Asn
580 585 590
Ile Thr Gly Trp Leu Asp Lys Asn Lys Asp Pro Leu Asn Glu Thr Val
595 600 605
Val Gly Leu Tyr Gln Lys Ser Gly Met Lys Thr Leu Ala Phe Leu Phe
610 615 620
Ser Gly Ala Gln Thr Gly Glu Glu Gly Gly Gly Gly Gly Lys Lys Gly
625 630 635 640
Gly Lys Lys Lys Gly Ser Ser Phe Gln Thr Val Ser Ala Leu Phe Arg
645 650 655
Glu Asn Leu Asn Lys Leu Met Thr Asn Leu Arg Ser Thr His Pro His
660 665 670
Phe Val Arg Cys Leu Ile Pro Asn Glu Thr Lys Thr Pro Gly Ala Met
675 680 685
Glu His Glu Leu Val Leu His Gln Leu Arg Cys Asn Gly Val Leu Glu
690 695 700
Gly Ile Arg Ile Cys Arg Lys Gly Phe Pro Ser Arg Ile Leu Tyr Ala
705 710 715 720
Asp Phe Lys Gln Arg Tyr Lys Val Leu Asn Ala Ser Ala Ile Pro Glu
725 730 735
Gly Gln Phe Ile Asp Ser Lys Lys Ala Ser Glu Lys Leu Leu Gly Ser
740 745 750
Ile Asp Val Asp His Thr Gln Tyr Lys Phe Gly His Thr Lys Val Phe
755 760 765
Phe Lys Ala Gly Leu Leu Gly Leu Leu Glu Glu Met Arg Asp Glu Lys
770 775 780
Leu Ala Gln Leu Ile Thr Arg Thr Gln Ala Ile Cys Arg Gly Tyr Leu
785 790 795 800
Thr Arg Val Glu Phe Lys Lys Met Met Glu Arg Arg Glu Ser Ile Phe
805 810 815
Cys Ile Gln Tyr Asn Ile Arg Ala Phe Met Asn Val Lys His Trp Pro
820 825 830
Trp Met Lys Leu Tyr Phe Lys Ile Lys Pro Leu Leu Lys Ser Ala Glu
835 840 845
Thr Glu Lys Glu Met Ala Asn Met Lys Glu Glu Phe Glu Lys Thr Lys
850 855 860
Glu Glu Leu Ala Lys Ser Glu Ala Lys Arg Lys Glu Leu Glu Glu Lys
865 870 875 880
Met Val Ala Leu Met Gln Glu Lys Asn Asp Leu Gln Leu Gln Val Gln
885 890 895
Ala Glu Ala Asp Gly Leu Ala Asp Ala Glu Glu Arg Cys Asp Gln Leu
900 905 910
Ile Lys Thr Lys Ile Gln Leu Glu Ala Lys Ile Lys Glu Val Thr Glu
915 920 925
Arg Ala Glu Asp Glu Glu Glu Ile Asn Ala Glu Leu Thr Ala Lys Lys
930 935 940
Arg Lys Leu Glu Asp Glu Cys Ser Glu Leu Lys Lys Asp Ile Asp Asp
945 950 955 960
Leu Glu Leu Thr Leu Ala Lys Val Glu Lys Glu Lys His Ala Thr Glu
965 970 975
Asn Lys Val Lys Asn Leu Thr Glu Glu Met Ala Gly Leu Asp Glu Asn
980 985 990
Ile Ala Lys Leu Thr Lys Glu Lys Lys Ala Leu Gln Glu Ala His Gln
995 1000 1005
Gln Thr Leu Asp Asp Leu Gln Ala Glu Glu Asp Lys Val Asn Thr
1010 1015 1020
Leu Thr Lys Ala Lys Thr Lys Leu Glu Gln Gln Val Asp Asp Leu
1025 1030 1035
Glu Gly Ser Leu Glu Gln Glu Lys Lys Leu Arg Met Asp Leu Glu
1040 1045 1050
Arg Ala Lys Arg Lys Leu Glu Gly Asp Leu Lys Leu Ala Gln Glu
1055 1060 1065
Ser Thr Met Asp Ile Glu Asn Asp Lys Gln Gln Leu Asp Glu Lys
1070 1075 1080
Leu Lys Lys Lys Glu Phe Glu Met Thr Asn Leu Gln Ser Lys Ile
1085 1090 1095
Glu Asp Glu Gln Ala Leu Ala Met Gln Leu Gln Lys Lys Ile Lys
1100 1105 1110
Glu Leu Gln Ala Arg Ile Glu Glu Leu Glu Glu Glu Ile Glu Ala
1115 1120 1125
Glu Arg Ala Ser Arg Ala Lys Ala Glu Lys Gln Arg Ser Asp Leu
1130 1135 1140
Ser Arg Glu Leu Glu Glu Ile Ser Glu Arg Leu Glu Glu Ala Gly
1145 1150 1155
Gly Ala Thr Ser Ala Gln Ile Glu Met Asn Lys Lys Arg Glu Ala
1160 1165 1170
Glu Phe Gln Lys Met Arg Arg Asp Leu Glu Glu Ala Thr Leu Gln
1175 1180 1185
His Glu Ala Thr Ala Ala Ala Leu Arg Lys Lys His Ala Asp Ser
1190 1195 1200
Val Ala Glu Leu Gly Glu Gln Ile Asp Asn Leu Gln Arg Val Lys
1205 1210 1215
Gln Lys Leu Glu Lys Glu Lys Ser Glu Leu Lys Met Glu Ile Asp
1220 1225 1230
Asp Leu Ala Gly Asn Met Glu Thr Val Ser Lys Ala Lys Gly Asn
1235 1240 1245
Leu Glu Lys Met Cys Arg Thr Leu Glu Asp Gln Leu Ser Glu Val
1250 1255 1260
Lys Thr Lys Glu Glu Glu His Gln Arg Leu Ile Asn Glu Leu Ser
1265 1270 1275
Ala Gln Lys Ala Arg Leu His Thr Glu Ser Gly Glu Phe Ser Arg
1280 1285 1290
Gln Leu Asp Glu Lys Asp Ala Met Val Ser Gln Leu Ser Arg Gly
1295 1300 1305
Lys Gln Ala Phe Thr Gln Gln Ile Glu Glu Leu Lys Arg Gln Leu
1310 1315 1320
Glu Glu Glu Thr Lys Ala Lys Ser Ala Leu Ala His Ala Leu Gln
1325 1330 1335
Ser Ser Arg His Asp Cys Asp Leu Leu Arg Glu Gln Tyr Glu Glu
1340 1345 1350
Glu Gln Glu Ala Lys Ala Glu Leu Gln Arg Ala Met Ser Lys Ala
1355 1360 1365
Asn Ser Glu Val Ser Gln Trp Arg Thr Lys Tyr Glu Thr Asp Ala
1370 1375 1380
Ile Gln Arg Thr Glu Glu Leu Glu Glu Ala Lys Lys Lys Leu Ala
1385 1390 1395
Gln Arg Leu Gln Asp Ala Glu Glu His Val Glu Ala Val Asn Ser
1400 1405 1410
Lys Cys Ala Ser Leu Glu Lys Thr Lys Gln Arg Leu Gln Asn Glu
1415 1420 1425
Val Glu Asp Leu Met Ile Asp Val Glu Arg Ser Asn Ala Ala Cys
1430 1435 1440
Ala Ala Leu Asp Lys Lys Gln Arg Asn Phe Asp Lys Val Leu Ala
1445 1450 1455
Glu Trp Lys His Lys Tyr Glu Glu Thr Gln Ala Glu Leu Glu Ala
1460 1465 1470
Ser Gln Lys Glu Ser Arg Ser Leu Ser Thr Glu Val Phe Lys Val
1475 1480 1485
Lys Asn Ala Tyr Glu Glu Ser Leu Asp His Leu Glu Thr Leu Lys
1490 1495 1500
Arg Glu Asn Lys Asn Leu Gln Gln Glu Ile Ser Asp Leu Thr Glu
1505 1510 1515
Gln Ile Ala Glu Ser Gly Lys His Ile His Glu Leu Glu Lys Val
1520 1525 1530
Lys Lys Gln Ile Asp Gln Glu Lys Ser Glu Leu Gln Ala Ala Leu
1535 1540 1545
Glu Glu Ala Glu Gly Ser Leu Glu His Glu Glu Gly Lys Ile Leu
1550 1555 1560
Arg Ile Gln Leu Glu Leu Asn Gln Val Lys Ser Glu Ile Asp Arg
1565 1570 1575
Lys Ile Ala Glu Lys Asp Glu Glu Ile Asp Gln Leu Lys Arg Asn
1580 1585 1590
His Leu Arg Val Val Glu Ser Met Gln Ser Thr Leu Asp Ala Glu
1595 1600 1605
Ile Arg Ser Arg Asn Asp Ala Leu Arg Ile Lys Lys Lys Met Glu
1610 1615 1620
Gly Asp Leu Asn Glu Met Glu Ile Gln Leu Asn His Ala Asn Arg
1625 1630 1635
Gln Ala Ala Glu Ala Ile Lys Asn Leu Arg Asn Thr Gln Gly Ile
1640 1645 1650
Leu Lys Asp Thr Gln Leu His Leu Asp Asp Ala Val Arg Gly Gln
1655 1660 1665
Asp Asp Leu Lys Glu Gln Leu Ala Met Val Glu Arg Arg Ala Asn
1670 1675 1680
Leu Met Gln Ala Glu Ile Glu Glu Leu Arg Ala Ser Leu Glu Gln
1685 1690 1695
Thr Glu Arg Ser Arg Arg Val Ala Glu Gln Glu Leu Leu Asp Ala
1700 1705 1710
Ser Glu Arg Val Gln Leu Leu His Thr Gln Asn Thr Ser Leu Ile
1715 1720 1725
Asn Thr Lys Lys Lys Leu Glu Thr Asp Ile Ser Gln Ile Gln Gly
1730 1735 1740
Glu Met Glu Asp Ile Val Gln Glu Ala Arg Asn Ala Glu Glu Lys
1745 1750 1755
Ala Lys Lys Ala Ile Thr Asp Ala Ala Met Met Ala Glu Glu Leu
1760 1765 1770
Lys Lys Glu Gln Asp Thr Ser Ala His Leu Glu Arg Met Lys Lys
1775 1780 1785
Asn Met Glu Gln Thr Val Lys Asp Leu Gln His Arg Leu Asp Glu
1790 1795 1800
Ala Glu Gln Leu Ala Leu Lys Gly Gly Lys Lys Gln Ile Gln Lys
1805 1810 1815
Leu Glu Ala Arg Val Arg Glu Leu Glu Asn Glu Val Glu Ser Glu
1820 1825 1830
Gln Lys Arg Asn Val Glu Ala Val Lys Gly Leu Arg Lys His Glu
1835 1840 1845
Arg Arg Val Lys Glu Leu Thr Tyr Gln Thr Glu Glu Asp Arg Lys
1850 1855 1860
Asn Val Leu Arg Leu Gln Asp Leu Val Asp Lys Leu Gln Ser Lys
1865 1870 1875
Val Lys Ala Tyr Lys Arg Gln Ala Glu Glu Ala Glu Glu Gln Ser
1880 1885 1890
Asn Ile Asn Leu Ser Lys Phe Arg Lys Leu Gln His Glu Leu Glu
1895 1900 1905
Glu Ala Glu Glu Arg Ala Asp Ile Ala Glu Ser Gln Val Asn Lys
1910 1915 1920
Leu Arg Val Lys Ser Arg Asp Val His Ser Lys Val Ile Ser Glu
1925 1930 1935
Glu
<210> 12
<211> 377
<212> PRT
<213> 普通牛
<400> 12
Met Cys Asp Glu Asp Glu Thr Thr Ala Leu Val Cys Asp Asn Gly Ser
1 5 10 15
Gly Leu Val Lys Ala Gly Phe Ala Gly Asp Asp Ala Pro Arg Ala Val
20 25 30
Phe Pro Ser Ile Val Gly Arg Pro Arg His Gln Gly Val Met Val Gly
35 40 45
Met Gly Gln Lys Asp Ser Tyr Val Gly Asp Glu Ala Gln Ser Lys Lys
50 55 60
Gly Ile Leu Thr Leu Lys Tyr Pro Ile Glu His Gly Ile Ile Thr Asn
65 70 75 80
Trp Asp Asp Met Glu Lys Ile Trp His His Thr Phe Tyr Asn Glu Leu
85 90 95
Arg Val Ala Pro Glu Glu His Pro Thr Leu Leu Thr Glu Ala Pro Leu
100 105 110
Asn Pro Lys Ala Asn Arg Glu Lys Met Thr Gln Ile Met Phe Glu Thr
115 120 125
Phe Asn Val Pro Ala Met Tyr Val Ala Ile Gln Ala Val Leu Ser Leu
130 135 140
Tyr Ala Ser Gly Arg Thr Thr Gly Ile Val Leu Asp Ser Gly Asp Gly
145 150 155 160
Val Thr His Asn Val Pro Ile Tyr Glu Gly Tyr Ala Leu Pro His Ala
165 170 175
Ile Met Arg Leu Asp Leu Ala Gly Arg Asp Leu Thr Asp Tyr Leu Met
180 185 190
Lys Ile Leu Thr Glu Arg Gly Tyr Ser Phe Val Thr Thr Ala Glu Arg
195 200 205
Glu Ile Val Arg Asp Ile Lys Glu Lys Leu Cys Tyr Val Ala Leu Asp
210 215 220
Phe Glu Asn Glu Met Ala Thr Ala Ala Ser Ser Ser Ser Leu Glu Lys
225 230 235 240
Ser Tyr Glu Leu Pro Asp Gly Gln Val Ile Thr Ile Gly Asn Glu Arg
245 250 255
Phe Arg Cys Pro Glu Thr Leu Phe Gln Pro Ser Phe Ile Gly Met Glu
260 265 270
Ser Ala Gly Ile His Glu Thr Thr Tyr Asn Ser Ile Met Lys Cys Asp
275 280 285
Ile Asp Ile Arg Lys Asp Leu Tyr Ala Asn Asn Val Met Ser Gly Gly
290 295 300
Thr Thr Met Tyr Pro Gly Ile Ala Asp Arg Met Gln Lys Glu Ile Thr
305 310 315 320
Ala Leu Ala Pro Ser Thr Met Lys Ile Lys Ile Ile Ala Pro Pro Glu
325 330 335
Arg Lys Tyr Ser Val Trp Ile Gly Gly Ser Ile Leu Ala Ser Leu Ser
340 345 350
Thr Phe Gln Gln Met Trp Ile Thr Lys Gln Glu Tyr Asp Glu Ala Gly
355 360 365
Pro Ser Ile Val His Arg Lys Cys Phe
370 375
<210> 13
<211> 465
<212> PRT
<213> 野猪
<400> 13
Met Cys Asp Glu Asp Glu Thr Thr Ala Leu Val Cys Asp Asn Gly Ser
1 5 10 15
Gly Leu Val Lys Ala Gly Phe Ala Gly Asp Asp Ala Pro Arg Ala Val
20 25 30
Phe Pro Ser Ile Val Gly Arg Pro Arg His Gln Gly Val Met Val Gly
35 40 45
Met Gly Gln Lys Asp Ser Tyr Val Gly Asp Glu Ala Gln Ser Lys Arg
50 55 60
Gly Ile Leu Thr Leu Lys Tyr Pro Ile Glu His Gly Ile Ile Thr Asn
65 70 75 80
Trp Asp Asp Met Glu Lys Ile Trp His His Thr Phe Tyr Asn Glu Leu
85 90 95
Arg Val Ala Pro Glu Glu His Pro Thr Leu Leu Thr Glu Ala Pro Leu
100 105 110
Asn Pro Lys Ala Asn Arg Glu Lys Met Thr Gln Ile Met Phe Glu Thr
115 120 125
Phe Asn Val Pro Ala Met Tyr Val Ala Ile Gln Ala Val Leu Ser Leu
130 135 140
Tyr Ala Ser Gly Arg Thr Thr Gly Ile Val Leu Asp Ser Gly Asp Gly
145 150 155 160
Val Thr His Asn Val Pro Ile Tyr Glu Gly Tyr Ala Leu Pro His Ala
165 170 175
Ile Met Arg Leu Asp Leu Ala Gly Arg Asp Leu Thr Asp Tyr Leu Met
180 185 190
Lys Ile Leu Thr Glu Arg Gly Tyr Ser Phe Val Thr Thr Ala Glu Arg
195 200 205
Glu Ile Val Arg Asp Ile Lys Glu Lys Leu Cys Tyr Val Ala Leu Asp
210 215 220
Phe Glu Asn Glu Met Ala Thr Ala Ala Ser Ser Ser Ser Leu Glu Lys
225 230 235 240
Ser Tyr Glu Leu Pro Asp Gly Gln Val Ile Thr Ile Gly Asn Glu Arg
245 250 255
Phe Arg Cys Pro Glu Thr Leu Phe Gln Pro Ser Phe Ile Gly Met Glu
260 265 270
Ser Ala Gly Ile His Glu Thr Thr Tyr Asn Ser Ile Met Lys Cys Asp
275 280 285
Ile Asp Ile Arg Lys Asp Leu Tyr Ala Asn Asn Val Met Ser Gly Gly
290 295 300
Thr Thr Met Tyr Pro Gly Ile Ala Asp Arg Met Gln Lys Glu Ile Thr
305 310 315 320
Ala Leu Ala Pro Ser Thr Met Lys Ile Lys Val Gly Gly Gly Pro Arg
325 330 335
Pro Cys Pro Leu Val Gly Ser Arg Leu Arg Leu Arg Pro Pro Asp Arg
340 345 350
Leu Ser Leu Ser Tyr Arg Ser Ser Pro Arg Arg Ser Ala Ser Thr Pro
355 360 365
Cys Gly Ser Ala Ala Pro Ser Trp Pro Arg Cys Pro Pro Ser Ser Arg
370 375 380
Cys Gly Ser Pro Ser Arg Ser Thr Thr Arg Pro Ala His Leu Ser Ser
385 390 395 400
Thr Ala Asn Ala Ser Lys Pro His Ser Asn Ser Cys Asp Phe Ser Ser
405 410 415
Gly Arg Arg Ile Cys Ser Ser Ala Val Ala Asp Leu Glu Pro Pro Ser
420 425 430
Gly Asn Cys Ser Pro Thr Ala Cys Val Ala Ala Ala Gln Thr Asp Thr
435 440 445
Met Phe Ile Thr Cys Thr Tyr Ile Asn Leu Phe Thr Ser Phe Cys Tyr
450 455 460
Phe
465
<210> 14
<211> 1175
<212> DNA
<213> 原鸡
<400> 14
ctcgagaaaa gaatgcacca tcatcatcat cactgcgacg aagacgaaac aactgcctta 60
gtatgcgata acggttcagg tttagtcaaa gccggttttg ctggtgatga tgctccaaga 120
gcagtttttc catctattgt tggtagacca agacatcaag gtgttatggt tggtatgggt 180
caaaaagatt cttatgttgg tgatgaagca caatcaaaga gaggtatctt gactttgaag 240
tacccaatcg aacatggtat catcacaaac tgggatgata tggaaaagat ctggcatcat 300
actttctaca acgaattaag agttgctcca gaagaacatc caactttgtt aacagaagct 360
ccattgaacc caaaggcaaa cagagaaaag atgactcaaa tcatgttcga aacattcaac 420
gttccagcta tgtacgttgc tattcaagca gttttgtctt tatacgcatc aggtagaact 480
acaggtattg ttttagattc aggtgatggt gttactcata acgttccaat ctacgaaggt 540
tacgctttgc cacatgcaat tatgagattg gatttagctg gtagagattt gactgattac 600
ttgatgaaga tcttgacaga aagaggttac tctttcgtta ctacagcaga aagagaaatc 660
gttagagata tcaaggaaaa gttgtgttat gttgctttgg attttgaaaa tgaaatggca 720
acagctgcat cttcatcttc attagaaaaa tcatacgaat tgccagatgg tcaagttatc 780
actatcggta acgaaagatt cagatgtcca gaaacattgt tccaaccatc tttcatcggt 840
atggaatcag ctggtatcca tgaaactaca tacaactcta tcatgaaatg tgatattgat 900
attagaaaag atttatatgc taataatgtt atgtcaggtg gtactacaat gtacccaggt 960
atcgcagata gaatgcaaaa ggaaatcact gctttggcac catctacaat gaagatcaag 1020
atcatcgctc caccagaaag aaaatactct gtttggattg gtggttcaat cttggcatct 1080
ttgtcaacat tccaacaaat gtggattacc aagcaagaat acgacgaagc aggtccatca 1140
atagttcaca gaaagtgttt ttaatgagcg gccgc 1175
<210> 15
<211> 3341
<212> DNA
<213> 原鸡
<400> 15
ctcgagaaaa gaatgcacca ccaccaccac cacttattga aatctgctga atccgaaaag 60
gaaatggcta acatgaagga agaatttgaa aagaccaagg aagaattagc aaagtcagaa 120
gctaagagaa aggaattgga agaaaagatg gttgttttgt tgcaagaaaa gaatgatttg 180
caattacaag ttcaagcaga agctgattct ttagcagatg ctgaagaaag atgtgatcaa 240
ttgatcaaga ctaagatcca attagaagca aagatcaagg aagttacaga aagagctgaa 300
gatgaagaag aaatcaacgc agaattgact gctaagaaaa gaaagttaga agatgaatgt 360
tctgaattga agaaagatat cgatgatttg gaattaacat tggcaaaggt tgaaaaggaa 420
aaacatgcta ctgaaaacaa ggttaagaac ttgacagaag aaatggcagt tttggatgaa 480
actatcgcta agttgacaaa ggaaaagaaa gctttgcaag aagctcatca acaaactttg 540
gatgatttgc aagttgaaga agataaggtt aacactttga caaaggctaa gacaaagttg 600
gaacaacaag ttgatgattt ggaaggttca ttagaacaag aaaagaaatt gagaatggat 660
ttagaaagag caaagagaaa gttagaaggt gatttaaagt tggctcatga ttctatcatg 720
gatttggaaa acgataaaca acaattggat gaaaagttga aaaagaaaga tttcgaaatc 780
tcacaaattc aatctaaaat tgaagatgaa caagctttgg gtatgcaatt gcaaaagaaa 840
attaaggaat tacaagcaag aatcgaagaa ttggaagaag aaatcgaagc tgaaagaact 900
tcaagagcaa aagctgaaaa acatagagca gatttgtcaa gagaattaga agaaatctct 960
gaaagattag aagaagctgg tggtgcaaca gctgcacaaa ttgaaatgaa caagaaaaga 1020
gaagctgaat tccaaaagat gagaagagat ttggaagaag caactttaca acatgaagct 1080
acagctgcag ctttgagaaa gaaacatgca gattctactg ctgaattagg tgaacaaatc 1140
gataacttgc aaagagttaa gcaaaagtta gaaaaggaaa aatcagaatt gaaaatggaa 1200
atcgatgatt tggcttctaa catggaatct gtttcaaagg caaaggctaa cttggaaaag 1260
atgtgtagaa ctttggaaga tcaattatca gaaatcaaga caaaggaaga acaaaaccaa 1320
agaatgatca acgatttgaa cactcaaaga gctagattgc aaactgaaac aggcgaatat 1380
tctagacaag cagaagaaaa ggatgctttg atctcacaat tatctagagg taaacaaggt 1440
ttcacacaac aaatcgaaga attgaagaga catttagaag aagaaatcaa agcaaaaaat 1500
gcattggctc atgcattaca atctgctaga catgattgtg aattgttgag agaacaatac 1560
gaagaagaac aagaagctaa gggtgaattg caaagagcat tatcaaaggc taactctgaa 1620
gttgcacaat ggagaactaa atacgaaaca gatgctattc aaagaactga agaattagaa 1680
gaagctaaga aaaaattggc tcaaagattg caagatgctg aagaacatgt tgaagctgtt 1740
aacgcaaagt gtgcttcatt ggaaaagaca aagcaaagat tgcaaaacga agttgaagat 1800
ttgatggttg atgttgaaag atctaatgca gcttgtgcag ctttagataa gaagcaaaag 1860
aatttcgata agatcttggc agaatggaag caaaagtacg aagaaactca aacagaattg 1920
gaagcttcac aaaaagaatc tagatcattg tctactgaat tattcaagat gaagaacgca 1980
tacgaagaat ctttggatca tttggaaact ttgaagagag aaaataagaa cttgcaacaa 2040
gaaattgctg atttgacaga acaaattgca gaaggtggta aagctgttca tgaattagaa 2100
aaagttaaaa aacatgttga acaagaaaaa tcagaattgc aagcttcttt agaagaagct 2160
gaagcatcat tggaacatga agaaggcaag atcttgagat tgcaattaga attgaatcaa 2220
attaaatctg aaattgatag aaaaattgct gaaaaagatg aagaaatcga tcaattgaag 2280
agaaaccatt tgagaatcgt tgaatcaatg caatctacat tggatgcaga aatcagatca 2340
agaaacgaag ctttgagatt gaaaaagaaa atggagggtg atttaaacga aatggaaatc 2400
caattgtctc atgcaaacag aatggcagct gaagctcaaa agaatttgag aaacactcaa 2460
ggtacattga aggatactca aatccatttg gatgatgcat tgagaacaca agaagatttg 2520
aaggaacaag ttgctatggt tgaaagaaga gcaaacttgt tgcaagctga agttgaagaa 2580
ttgagaggtg ctttagaaca aactgaaaga tctagaaagg ttgcagaaca agaattgtta 2640
gatgctacag aaagagttca attgttacat actcaaaaca catcattgat caacactaag 2700
aaaaaattgg aaacagatat cgttcaaatc caatctgaaa tggaagatac tattcaagaa 2760
gcaagaaatg ctgaagaaaa agctaagaaa gctattacag atgcagctat gatggcagaa 2820
gaattgaaga aagaacaaga cacttctgct catttggaaa gaatgaagaa aaatatggat 2880
caaacagtta aggatttgca tgttagattg gatgaagcag aacaattggc tttgaaaggt 2940
ggtaaaaaac aattgcaaaa gttagaagct agagttagag aattagaagg tgaagttgat 3000
tcagaacaaa agagatctgc tgaagcagtt aagggtgtta gaaagtacga aagaagagtt 3060
aaggaattga cttaccaatg tgaagaagat agaaagaaca tcttgagatt gcaagatttg 3120
gttgataaat tacaaatgaa ggttaagtca tacaagagac aagcagaaga agctgaagaa 3180
ttgtcaaacg ttaacttgtc taagttcaga aagatccaac atgaattaga agaagctgaa 3240
gaaagagcag atattgctga atctcaagta aataagttga gagtaaagtc cagagaaatc 3300
cacggtaaaa agatagaaga agaagaataa tgagcggccg c 3341
<210> 16
<211> 1940
<212> PRT
<213> 欧洲大扇贝
<400> 16
Met Asn Ile Asp Tyr Ser Asp Pro Asp Phe Gln Tyr Leu Ala Val Asp
1 5 10 15
Arg Lys Lys Leu Met Lys Glu Gln Thr Ala Ala Phe Asp Gly Lys Lys
20 25 30
Asn Cys Trp Val Pro Asp Glu Lys Glu Gly Phe Ala Pro Ala Glu Ile
35 40 45
Gln Ser Ser Lys Gly Asp Glu Ile Thr Val Lys Ile Thr Ser Asp Asn
50 55 60
Ser Thr Arg Thr Val Lys Lys Asp Asp Ile Gln Ser Met Asn Pro Pro
65 70 75 80
Lys Phe Glu Lys Leu Glu Asp Met Ala Asn Met Thr Tyr Leu Asn Glu
85 90 95
Ala Ser Val Leu His Asn Leu Arg Ser Arg Tyr Thr Ser Gly Leu Ile
100 105 110
Tyr Thr Tyr Ser Gly Leu Phe Cys Ile Ala Val Asn Pro Tyr Arg Arg
115 120 125
Leu Pro Ile Tyr Thr Asp Ser Val Ile Ser Lys Tyr Arg Gly Lys Arg
130 135 140
Lys Thr Glu Ile Pro Pro His Leu Phe Ser Val Ala Asp Asn Ala Tyr
145 150 155 160
Gln Asn Met Val Thr Asp Arg Glu Asn Gln Ser Cys Leu Ile Thr Gly
165 170 175
Glu Ser Gly Ala Gly Lys Thr Glu Asn Thr Lys Lys Val Ile Met Tyr
180 185 190
Leu Ala Lys Val Ala Cys Ala Val Lys Lys Lys Thr Asp Glu Glu Glu
195 200 205
Ala Ser Asp Lys Lys Gln Gly Ser Leu Glu Asp Gln Ile Ile Gln Ala
210 215 220
Asn Pro Val Leu Glu Ala Tyr Gly Asn Ala Lys Thr Thr Arg Asn Asn
225 230 235 240
Asn Ser Ser Arg Phe Gly Lys Phe Ile Arg Ile His Phe Gly Pro Thr
245 250 255
Gly Lys Ile Ala Gly Ala Asp Ile Glu Thr Tyr Leu Leu Glu Lys Ser
260 265 270
Arg Val Thr Tyr Gln Gln Ser Ala Glu Arg Asn Tyr His Ile Phe Tyr
275 280 285
Gln Val Cys Ser Asn Ala Leu Pro Glu Leu Asn Asp Ile Met Leu Val
290 295 300
Thr Pro Asp Ser Gly Leu Tyr Ser Phe Ile Asn Gln Gly Cys Leu Thr
305 310 315 320
Val Asp Asn Ile Asp Asp Val Glu Glu Phe Lys Leu Cys Asp Glu Ala
325 330 335
Phe Asp Ile Leu Gly Phe Thr Lys Glu Glu Lys Gln Ser Met Phe Lys
340 345 350
Cys Thr Ala Ser Ile Leu His Met Gly Glu Met Lys Phe Lys Gln Arg
355 360 365
Pro Arg Glu Glu Gln Ala Glu Ser Asp Gly Thr Ala Glu Ala Glu Lys
370 375 380
Val Ala Phe Leu Cys Gly Ile Asn Ala Gly Asp Leu Leu Lys Ala Leu
385 390 395 400
Leu Lys Pro Lys Val Lys Val Gly Thr Glu Met Val Thr Lys Gly Gln
405 410 415
Asn Leu Asn Gln Val Thr Asn Ser Val Gly Ala Leu Ala Lys Ser Leu
420 425 430
Tyr Asp Arg Met Phe Asn Trp Leu Val Lys Arg Val Asn Lys Thr Leu
435 440 445
Asp Thr Lys Ala Lys Arg Asn Tyr Tyr Ile Gly Val Leu Asp Ile Ala
450 455 460
Gly Phe Glu Ile Phe Asp Tyr Asn Ser Phe Glu Gln Leu Cys Ile Asn
465 470 475 480
Tyr Thr Asn Glu Arg Leu Gln Gln Phe Phe Asn His His Met Phe Ile
485 490 495
Leu Glu Gln Glu Glu Tyr Lys Lys Glu Gly Ile Ala Trp Glu Phe Ile
500 505 510
Asp Phe Gly Met Asp Leu Gln Met Cys Ile Asp Leu Ile Glu Lys Pro
515 520 525
Met Gly Ile Leu Ser Ile Leu Glu Glu Glu Cys Met Phe Pro Lys Ala
530 535 540
Asp Asp Lys Ser Phe Gln Asp Lys Leu Tyr Gln Asn His Met Gly Lys
545 550 555 560
Asn Arg Met Phe Thr Lys Pro Gly Lys Pro Thr Arg Pro Asn Gln Gly
565 570 575
Pro Ala His Phe Glu Leu His His Tyr Ala Gly Asn Val Pro Tyr Ser
580 585 590
Ile Thr Gly Trp Leu Asp Lys Asn Lys Asp Pro Ile Asn Glu Asn Val
595 600 605
Val Ser Leu Leu Ser Val Ser Lys Glu Pro Leu Val Ala Glu Leu Phe
610 615 620
Arg Ala Pro Glu Glu Pro Val Gly Gly Gly Gly Lys Lys Lys Lys Gly
625 630 635 640
Lys Ser Ser Ala Phe Gln Thr Ile Ser Ala Val His Arg Glu Ser Leu
645 650 655
Asn Lys Leu Met Lys Asn Leu Tyr Ser Thr His Pro Ser Phe Val Arg
660 665 670
Cys Ile Ile Pro Asn Glu Leu Lys Gln Pro Gly Leu Val Asp Ala Glu
675 680 685
Leu Val Leu His Gln Leu Gln Cys Asn Gly Val Leu Glu Gly Ile Arg
690 695 700
Ile Cys Arg Lys Gly Phe Pro Ser Arg Leu Ile Tyr Ser Glu Phe Lys
705 710 715 720
Gln Arg Tyr Ser Ile Leu Ala Pro Asn Ala Ile Pro Gln Gly Phe Val
725 730 735
Asp Gly Lys Thr Val Ser Glu Lys Ile Leu Thr Gly Leu Gln Met Asp
740 745 750
Pro Ala Glu Tyr Arg Leu Gly Thr Thr Lys Val Phe Phe Lys Ala Gly
755 760 765
Val Leu Gly Asn Leu Glu Glu Met Arg Asp Glu Arg Leu Ser Lys Ile
770 775 780
Ile Ser Met Phe Gln Ala His Ile Arg Gly Tyr Leu Ile Arg Lys Ala
785 790 795 800
Tyr Lys Lys Leu Gln Asp Gln Arg Ile Gly Leu Ser Val Ile Gln Arg
805 810 815
Asn Ile Arg Lys Trp Leu Val Leu Arg Asn Trp Gln Trp Trp Lys Leu
820 825 830
Tyr Ala Lys Val Lys Pro Leu Leu Ser Ile Ala Arg Gln Glu Glu Glu
835 840 845
Met Lys Glu Gln Leu Lys Gln Met Asp Lys Met Lys Glu Asp Leu Ala
850 855 860
Lys Thr Glu Arg Ile Lys Lys Glu Leu Glu Glu Gln Asn Val Thr Leu
865 870 875 880
Leu Glu Gln Lys Asn Asp Leu Phe Leu Gln Leu Gln Thr Ile Glu Asp
885 890 895
Ser Met Gly Asp Gln Glu Glu Arg Val Glu Lys Leu Ile Met Gln Lys
900 905 910
Ala Asp Phe Glu Ser Gln Ile Lys Glu Leu Glu Glu Arg Leu Leu Asp
915 920 925
Glu Glu Asp Ala Ala Ala Asp Leu Glu Gly Ile Lys Lys Lys Met Glu
930 935 940
Thr Asp Asn Ser Asn Leu Lys Lys Asp Ile Gly Asp Leu Glu Asn Thr
945 950 955 960
Leu Gln Lys Ala Glu Gln Asp Lys Ala His Lys Asp Asn Gln Ile Ser
965 970 975
Thr Leu Gln Gly Glu Met Ala Gln Gln Asp Glu His Ile Gly Lys Leu
980 985 990
Asn Lys Glu Lys Lys Ala Leu Glu Glu Ala Asn Lys Lys Thr Ser Glu
995 1000 1005
Ser Leu Gln Ala Glu Glu Asp Lys Cys Asn His Leu Asn Lys Leu
1010 1015 1020
Lys Ala Lys Leu Glu Gln Ala Leu Asp Glu Leu Glu Asp Asn Leu
1025 1030 1035
Glu Arg Glu Lys Lys Val Arg Gly Asp Val Glu Lys Ala Lys Arg
1040 1045 1050
Lys Val Glu Gln Asp Leu Lys Ser Thr Gln Glu Asn Val Glu Asp
1055 1060 1065
Leu Glu Arg Val Lys Arg Glu Leu Glu Glu Asn Val Arg Arg Lys
1070 1075 1080
Glu Ala Glu Ile Ser Thr Leu Asn Ser Lys Leu Glu Asp Glu Gln
1085 1090 1095
Asn Leu Val Ser Gln Leu Gln Arg Lys Val Lys Glu Leu Gln Ala
1100 1105 1110
Arg Ile Glu Glu Leu Glu Glu Glu Leu Glu Ala Glu Arg Asn Ala
1115 1120 1125
Arg Ala Lys Val Glu Lys Gln Arg Ala Glu Leu Asn Arg Glu Leu
1130 1135 1140
Glu Glu Leu Gly Glu Arg Leu Asp Glu Ala Gly Gly Ala Thr Ser
1145 1150 1155
Ala Gln Ile Glu Leu Asn Lys Lys Arg Glu Ala Glu Leu Leu Lys
1160 1165 1170
Ile Arg Arg Asp Leu Glu Glu Ala Ser Leu Gln His Glu Ala Gln
1175 1180 1185
Ile Ser Ala Leu Arg Lys Lys His Gln Asp Ala Ala Asn Glu Met
1190 1195 1200
Ala Asp Gln Val Asp Gln Leu Gln Lys Val Lys Ser Lys Leu Glu
1205 1210 1215
Lys Asp Lys Lys Asp Ile Lys Arg Glu Met Asp Asp Leu Glu Ser
1220 1225 1230
Gln Met Thr His Asn Met Lys Asn Lys Gly Cys Ser Glu Lys Val
1235 1240 1245
Met Lys Gln Phe Glu Ser Gln Met Ser Asp Leu Asn Ala Arg Leu
1250 1255 1260
Glu Asp Ser Gln Arg Ser Ile Asn Glu Leu Gln Ser Gln Lys Ser
1265 1270 1275
Arg Leu Gln Ala Glu Asn Ser Asp Leu Ser Arg Gln Leu Glu Asp
1280 1285 1290
Ala Glu His Arg Val Ser Val Leu Ser Lys Glu Lys Ser Gln Leu
1295 1300 1305
Gly Ser Gln Leu Glu Asp Ala Arg Arg Ser Leu Glu Asp Glu Thr
1310 1315 1320
Arg Ala Arg Ser Lys Leu Gln Asn Glu Val Arg Asn Met His Ala
1325 1330 1335
Asp Met Asp Ala Val Arg Glu Gln Leu Glu Glu Glu Gln Glu Ser
1340 1345 1350
Lys Ser Asp Val Gln Arg Gln Leu Ser Lys Ala Asn Asn Glu Ile
1355 1360 1365
Gln Gln Trp Arg Ser Lys Phe Glu Ser Glu Gly Ala Asn Arg Thr
1370 1375 1380
Glu Glu Leu Glu Asp Gln Lys Arg Lys Ile Leu Gly Lys Leu Ser
1385 1390 1395
Glu Ala Glu Gln Thr Thr Glu Ala Ala Asn Ser Lys Cys Ser Ala
1400 1405 1410
Leu Glu Lys Ala Lys Ser Arg Leu Gln Gln Glu Leu Glu Asp Met
1415 1420 1425
Ser Ile Glu Val Asp Arg Ala Asn Ala Asn Val Asn Gln Met Glu
1430 1435 1440
Lys Lys Gln Arg Ala Phe Asp Lys Thr Thr Ala Glu Trp Gln Ser
1445 1450 1455
Lys Val Asn Ser Leu Gln Ser Glu Leu Glu Asn Ala Gln Lys Glu
1460 1465 1470
Ser Arg Gly Tyr Ser Ala Glu Leu Tyr Arg Ile Lys Ala Ser Val
1475 1480 1485
Glu Glu Tyr Gln Asp Ser Ile Gly Ser Leu Arg Arg Glu Asn Lys
1490 1495 1500
Asn Leu Ala Asp Glu Ile His Asp Leu Thr Asp Gln Leu Ser Glu
1505 1510 1515
Gly Gly Arg Ser Thr His Glu Leu Asp Lys Ala Arg Arg Arg Leu
1520 1525 1530
Glu Met Glu Lys Glu Glu Leu Gln Ala Ala Leu Glu Glu Ala Glu
1535 1540 1545
Gly Ala Leu Glu Gln Glu Glu Ala Lys Val Met Arg Ala Gln Leu
1550 1555 1560
Glu Ile Ala Thr Val Arg Asn Glu Ile Asp Lys Arg Ile Gln Glu
1565 1570 1575
Lys Glu Glu Glu Phe Asp Asn Thr Arg Arg Asn His Gln Arg Ala
1580 1585 1590
Leu Glu Ser Met Gln Ala Ser Leu Glu Ala Glu Ala Lys Gly Lys
1595 1600 1605
Ala Asp Ala Met Arg Ile Lys Lys Lys Leu Glu Gln Asp Ile Asn
1610 1615 1620
Glu Leu Glu Val Ala Leu Asp Ala Ser Asn Arg Gly Lys Ala Glu
1625 1630 1635
Met Glu Lys Thr Val Lys Arg Tyr Gln Gln Gln Ile Arg Glu Met
1640 1645 1650
Gln Thr Ser Ile Glu Glu Glu Gln Arg Gln Arg Asp Glu Ala Arg
1655 1660 1665
Glu Ser Tyr Asn Met Ala Glu Arg Arg Cys Thr Leu Met Ser Gly
1670 1675 1680
Glu Val Glu Glu Leu Arg Ala Ala Leu Glu Gln Ala Glu Arg Ala
1685 1690 1695
Arg Lys Ala Ser Asp Asn Glu Leu Ala Asp Ala Asn Asp Arg Val
1700 1705 1710
Asn Glu Leu Thr Ser Gln Val Ser Ser Val Gln Gly Gln Lys Arg
1715 1720 1725
Lys Leu Glu Gly Asp Ile Asn Ala Met Gln Thr Asp Leu Asp Glu
1730 1735 1740
Met His Gly Glu Leu Lys Gly Ala Asp Glu Arg Cys Lys Lys Ala
1745 1750 1755
Met Ala Asp Ala Ala Arg Leu Ala Asp Glu Leu Arg Ala Glu Gln
1760 1765 1770
Asp His Ser Ser Gln Val Glu Lys Val Arg Lys Asn Leu Glu Ser
1775 1780 1785
Gln Val Lys Glu Phe Gln Ile Arg Leu Asp Glu Ala Glu Ala Ser
1790 1795 1800
Ser Leu Lys Gly Gly Lys Lys Met Ile Gln Lys Leu Glu Ser Arg
1805 1810 1815
Val His Glu Leu Glu Ala Glu Leu Asp Asn Glu Gln Arg Arg His
1820 1825 1830
Ala Glu Thr Gln Lys Asn Met Arg Lys Ala Asp Arg Arg Leu Lys
1835 1840 1845
Glu Leu Ala Phe Gln Ala Asp Glu Asp Arg Lys Asn Gln Glu Arg
1850 1855 1860
Leu Gln Glu Leu Ile Asp Lys Leu Asn Ala Lys Ile Lys Thr Phe
1865 1870 1875
Lys Arg Gln Val Glu Glu Ala Glu Glu Ile Ala Ala Ile Asn Leu
1880 1885 1890
Ala Lys Tyr Arg Lys Ala Gln His Glu Leu Glu Glu Ala Glu Glu
1895 1900 1905
Arg Ala Asp Thr Ala Asp Ser Thr Leu Gln Lys Phe Arg Ala Lys
1910 1915 1920
Ser Arg Ser Ser Val Ser Val Gln Arg Ser Ser Val Ser Val Ser
1925 1930 1935
Ala Asn
1940

Claims (19)

1.一种通过在可食用生物宿主中重组表达肌肉蛋白来生产合成肉的新方法包括以下步骤：
a)将重组源材料接种到生长培养基中；
b)在酵母或细菌或其他可食用生物宿主存在下，在基础生长培养基上在发酵过程中培养上述重组源材料，以形成结构肌肉蛋白；
c)繁殖酵母菌和细菌，使其大量生长；
d)使用生化和化学方法将上述结构肌肉蛋白交联成用于生产蛋白质的纤维，用于生产蛋白质纤维；
e)将上述交联纤维束缚在一起，以提供强度和硬度；
f)将上述获得的蛋白质纤维与胶原蛋白、水和脂肪混合，以生产所需的合成肉；
其中，所得动物肉的特征在于由胶原蛋白、水、肌肉蛋白、结缔组织脂肪和血球蛋白组成，并且除了用其他必需营养素强化之外，还能进一步与基于植物的蛋白质混合。
2.根据权利要求1所述的新方法，其中，可食用生物宿主选自丝状真菌，所述丝状真菌包括可食物种，诸如Fusarium venenatum、米曲霉、紫红曲霉、米根霉、间型脉孢菌、木霉属；这些蘑菇包括小型褐菇、大型顶蘑菇和纽扣蘑菇、羊肚菌、香菇、平菇、金针菇，以及牛肝菌。
3.根据权利要求1所述的新方法，其中细胞包含编码含有多肽的肌肉蛋白的核酸，所述多肽选自肌动蛋白、肌球蛋白、截短肌动蛋白、截短肌球蛋白及其组合。
4.根据权利要求1所述的新方法，其中细胞包含编码截短肌肉肌球蛋白的核酸，所述肌肉肌球蛋白是卷曲螺旋。
5.根据权利要求1所述的新方法，其中细胞包含启动子序列，所述启动子序列是组成性启动子。
6.根据权利要求1所述的新方法，其中细胞包含所述启动子序列，所述启动子序列为组织特异性启动子。
7.根据权利要求1所述的新方法，其中细胞包含所述启动子序列，所述启动子序列为可诱导启动子。
8.根据权利要求1所述的新方法，其中细胞进一步包含转录终止区。
9.根据权利要求1所述的新方法，其中细胞包含信号肽，所述信号肽为分泌信号肽。
10.根据权利要求1所述的新方法，其中，所述酵母选自酿酒酵母、包括毕赤酵母、假丝酵母属、Komagataella属、乳克鲁维酵母、解脂耶氏酵母、东方伊萨酵母属在内的毕赤酵母属或其混合物。
11.根据权利要求1所述的新方法，其中，所述细菌选自链球菌、乳酸杆菌、芽孢杆菌、大肠杆菌、沙门氏菌、奈瑟氏菌、醋酸杆菌、节杆菌、曲霉、双歧杆菌、棒状杆菌、荧光假单胞菌或其混合物。
12.根据权利要求1所述的新方法，其中，所述结构肌肉蛋白来自动物种的肌球蛋白II和肌肉肌动蛋白，所述动物物种为诸如鸭子(鸭属)、兔子(真兔属)、鹅(雁属)、野牛和水牛(北美野牛、水牛属)、绵羊(绵羊属)、山羊(山羊属)、马(马属)、鱼类(太平洋鲑属、罗非鱼属、鳕属、红点鲑属，狼鲈属，银鲈属、鲶属、鰤属，鲈属、扇贝(扇贝属)、虾(滨对虾属，对虾属、毛虾属、鹰爪虾属、明对虾属、北极甜虾)，螃蟹(滨蟹属、蓝蟹、首长黄道蟹、北太平洋雪蟹)、牡蛎和贻贝(翼形亚纲)、鸡(原鸡)、猪(野猪)和牛(普通牛)。
13.根据权利要求1所述的新方法，其中，产生的纤维可来自一组“长链”蛋白质，诸如在头发、肌肉或鞭毛中发现的蛋白质。
14.根据权利要求1所述的新方法，其中，基于植物的蛋白质的混合物可选自鹰嘴豆、花生、杏仁、扁豆等。
15.根据权利要求1所述的新方法，其中，所得动物肉可添加选自ω-3脂肪酸的营养素，以增加其营养价值。
16.根据权利要求1所述的新方法，其中，所得动物肉可用于培根、火腿、热狗、意大利熏火腿、香肠、宠物食品、动物饲料等；此外，基于植物的蛋白质可以是从植物获得的食物蛋白源。
17.根据权利要求1所述的新方法，其中，所得肉类在外观、味道、气味、质构或其他因素上可能与传统生产的肉类相似。
18.根据权利要求1所述的新方法，其中，与传统肉类相比，所述动物肉具有潜在的健康、环境、文化和经济考虑。
19.根据权利要求1所述的新方法，其中，改进的方法有助于显著减少屠宰动物。
CN202180006833.7A 2020-01-02 2021-01-04 一种制造合成肉的新方法 Pending CN114929256A (zh)

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WO2023227743A1 (en) * 2022-05-25 2023-11-30 Bumble Be Gmbh Mycelial food substitute and a method of its production
JP7426764B1 (ja) 2023-06-05 2024-02-02 ＮＵＰｒｏｔｅｉｎ株式会社 タンパク質の生産方法、培養肉の生産方法、培養肉の生産方法に用いる添加物、および、タンパク質の生産方法に用いるキット

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AU2004324099B2 (en) * 2004-09-17 2011-02-03 Jon Vein Tissue engineered meat for consumption and a method for producing tissue engineered meat for consumption
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JP2017505138A (ja) * 2014-02-05 2017-02-16 モダン メドー インコーポレイテッド 培養筋細胞から形成される乾燥食品
CN103892273A (zh) * 2014-04-21 2014-07-02 宁夏伊味清真食品有限公司 一种清真素肉肠的生产方法
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US20220228121A1 (en) * 2019-05-31 2022-07-21 Trustees Of Tufts College Cultured meat product with genetically modified cells
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