CN114924042B - Data acquisition method based on Internet of things - Google Patents
Data acquisition method based on Internet of things Download PDFInfo
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- CN114924042B CN114924042B CN202210577880.3A CN202210577880A CN114924042B CN 114924042 B CN114924042 B CN 114924042B CN 202210577880 A CN202210577880 A CN 202210577880A CN 114924042 B CN114924042 B CN 114924042B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0004—Gaseous mixtures, e.g. polluted air
- G01N33/0009—General constructional details of gas analysers, e.g. portable test equipment
- G01N33/0062—General constructional details of gas analysers, e.g. portable test equipment concerning the measuring method, e.g. intermittent, or the display, e.g. digital
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0004—Gaseous mixtures, e.g. polluted air
- G01N33/0009—General constructional details of gas analysers, e.g. portable test equipment
- G01N33/0027—General constructional details of gas analysers, e.g. portable test equipment concerning the detector
- G01N33/0036—Specially adapted to detect a particular component
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16Y—INFORMATION AND COMMUNICATION TECHNOLOGY SPECIALLY ADAPTED FOR THE INTERNET OF THINGS [IoT]
- G16Y40/00—IoT characterised by the purpose of the information processing
- G16Y40/10—Detection; Monitoring
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16Y—INFORMATION AND COMMUNICATION TECHNOLOGY SPECIALLY ADAPTED FOR THE INTERNET OF THINGS [IoT]
- G16Y40/00—IoT characterised by the purpose of the information processing
- G16Y40/20—Analytics; Diagnosis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P90/00—Enabling technologies with a potential contribution to greenhouse gas [GHG] emissions mitigation
- Y02P90/02—Total factory control, e.g. smart factories, flexible manufacturing systems [FMS] or integrated manufacturing systems [IMS]
Abstract
The invention discloses a data acquisition method based on the Internet of things, which relates to the technical field of data acquisition and comprises the following steps: preparation before acquisition, test piece installation, multi-region sampling, sampling acquisition and data analysis. According to the invention, the sampling equipment is arranged on the transmission chain, so that the microbial colony which is free in a horizontal state and a spray flying state can be effectively collected, and the accuracy of the subsequently obtained microbial colony image data is ensured; along with the sampling of the sampling equipment driven by the transmission belt in different intervals, after each sampling is finished, the sampling test piece can stretch into the sterile sampling box again and the sealing side plate is closed immediately, so that even if the sampling equipment shuttles into the atmosphere environment and the processing sampling interval, the data can be automatically acquired, microorganisms in the atmosphere environment are ensured not to diffuse into the processing interval, and the deviation of the acquired data and the real data is further reduced.
Description
Technical Field
The invention relates to the technical field of data acquisition, in particular to a data acquisition method based on the Internet of things.
Background
In a food and drug processing factory, the requirement on the production and processing environment is high, the detection of microbial colonies is needed to be carried out on the atmospheric environment in a production area at regular intervals, the variety is various, the working procedure is complex, the data acquisition is needed to be carried out on a plurality of processing areas, and the bacterial sampling test pieces are respectively placed at specific acquisition positions of each processing area by operators wearing dust-free clothes in the prior art.
However, in the long-term data acquisition and measurement process, certain defects are found in the prior art: 1. because the sampling test piece is directly horizontally arranged in the sampling interval, only falling microbial colonies in the environment can be collected, and microbial colonies which are free in a horizontal state and a spray flying state are not effectively collected, so that the obtained microbial colony image data is inaccurate; 2. the processing regions are not necessarily in the same closed region, when the processing regions cross the atmosphere, operators also need to frequently put on and take off dust-free clothes, the efficiency is low, and microorganisms in the atmosphere are difficult to ensure not to diffuse to the processing regions, so that the acquired data and the real data have deviation.
Disclosure of Invention
The invention aims to provide a data acquisition method based on the Internet of things, which aims to solve the defects caused by the prior art.
A data acquisition method based on the Internet of things comprises the following steps:
s1: preparation before acquisition: sterilizing the sampling equipment;
s2: and (3) mounting a test piece: filling a plurality of bacterial sampling test pieces into a bearing box in a sampling device in a sterile operation mode, wherein the sampling test pieces are prepared culture medium systems in advance and comprise standard culture medium, cold water soluble gel and an indicator;
s3: multi-region sampling: the terminal of the Internet of things sends a remote acquisition request, the sampling equipment is driven to sequentially travel to an interval to be acquired through a transmission chain, and the acquisition time is kept for 3-5min each time;
s4: sampling and obtaining: driving the sampling equipment back to the sampling position again through the transmission chain;
s5: data analysis: and receiving a sampling test piece acquired in the sampling equipment, detecting a colony image, feeding back colony data, a corresponding microorganism growth environment and environmental information of a corresponding acquisition interval, and storing the colony data, the corresponding microorganism growth environment and the environmental information in an Internet of things terminal.
Preferably, the sampling equipment comprises an aseptic sampling box, a steering engine and prismatic absorption parts, the bearing box is arranged in the aseptic sampling box, the steering engine is also arranged in the aseptic sampling box, the output end of the steering engine is connected with a crank, the other end of the crank is slidably provided with a pushing part, the pushing part is slidably arranged in the aseptic sampling box, the side end of the pushing part is fixedly connected with two symmetrically arranged sliding rods, an installation frame is slidably arranged on the sliding rods, springs are sleeved between the installation frame and the pushing part on the sliding rods, the absorption parts are arranged on the installation frame, a negative pressure generator is arranged in the absorption parts on the installation frame, the other end of the installation frame is fixedly connected with a push rod, the upper end of the push rod is hinged with a pulling rod, and a tension spring is further connected between the pulling rod and the push rod.
Preferably, the sliding track on the pushing part is of a width changing and the middle part is of a ring shape.
Preferably, the absorbing member is provided with ventilation micropores, and the side end surface of the absorbing member is matched with the back of the sampling test piece.
Preferably, the sample collection groove is matched with the containing box in the vertical direction.
The invention has the advantages that:
(1) Through setting up the sampling equipment on the drive chain, form the suction by the suction generator in the prismatic table terminal surface of the suction attachment, after adsorbing the sample test piece that is pulled down, when the tip of the crank is in the major diameter circular orbit of the middle part of the pushing part, the sample test piece of the unsettled resting state collects the microbial colony from level, top-down and from bottom-up, realize the microorganism colony that is free in the level state and flying up the state can also be collected effectively, guarantee the accuracy of the microbial colony image data obtained subsequently at most;
(2) Along with the sampling of the sampling equipment driven by the transmission belt in different intervals, after each sampling is finished, the sampling test piece can stretch into the sterile sampling box again and the sealing side plate is closed immediately, so that even if the sampling equipment shuttles into the atmosphere environment and the processing sampling interval, the data can be automatically acquired, microorganisms in the atmosphere environment are ensured not to diffuse into the processing interval, and the deviation of the acquired data and the real data is further reduced.
Drawings
FIG. 1 is a process flow diagram of the present invention.
Fig. 2 is a side view of the structure of the device used in the present invention.
Fig. 3 is a schematic view of the sampling device of the present invention in an open state.
Fig. 4 is a schematic structural diagram of a sampling device according to the present invention.
Fig. 5 is an internal schematic view of a part of the structure of the sampling device according to the present invention.
FIG. 6 is a schematic view of a part of the structure of the aseptic sampling tank of the present invention.
The device comprises a 1-sampling device, a 2-sampling test piece, a 3-bearing box, a 4-transmission chain, a 101-sterile sampling box, a 102-steering engine, a 103-absorption part, a 104-crank, a 105-pushing part, a 106-sliding rod, a 107-mounting frame, a 108-spring, a 109-negative pressure generator, a 110-ejector rod, a 111-pulling rod, a 112-tension spring, a 1011-feeding cover plate, a 1012-sealing side plate, a 1014-protection isolation plate, a 1015-material taking cover plate and a 1016-sample collecting groove.
Detailed Description
The invention is further described in connection with the following detailed description, in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the invention easy to understand.
As shown in fig. 1 to 6, a data acquisition method based on the internet of things comprises the following steps:
s1: preparation before acquisition: sterilizing the sampling device 1;
s2: and (3) mounting a test piece: filling a plurality of bacterial sampling test pieces 2 into a containing box 3 in a sampling device in a sterile operation mode, wherein the sampling test pieces 2 are a prepared culture medium system and comprise a standard culture medium, cold water soluble gel and an indicator;
s3: multi-region sampling: the terminal of the Internet of things sends a remote acquisition request, the sampling equipment 1 is driven to sequentially travel to an interval to be acquired through the transmission chain 4, and the acquisition time is kept for 3-5min each time;
s4: sampling and obtaining: the sampling device 1 is back driven to the sampling position again through the transmission chain 4;
s5: data analysis: and receiving the sampling test piece 2 acquired in the sampling equipment 1, detecting colony images, feeding back colony data, corresponding microorganism growth environments and environmental information of corresponding acquisition intervals, and storing the colony data, the corresponding microorganism growth environments and the environmental information in the terminal of the Internet of things.
In the invention, the sampling device 1 is arranged in a suspended manner, and the microorganism spray can be captured in the horizontal direction and on the upper side and the lower side of the sampling device 1.
In this embodiment, the sampling device 1 includes aseptic sampling box 101, steering wheel 102 and prismatic table shape's adsorption piece 103, the holding box 3 is installed in aseptic sampling box 101, steering wheel 102 also installs in aseptic sampling box 101, and the output of steering wheel 102 is connected with crank 104, the other end slip of crank 104 is provided with pushing part 105, pushing part 105 slip sets up in aseptic sampling box 101, pushing part 105's side fixedly connected with slide bar 106 that two symmetries set up, slide bar 106 slip is provided with mounting bracket 107 on, and slide bar 106 is gone up in the cover between mounting bracket 107 and pushing part 105 and is equipped with spring 108, adsorption piece 103 is installed on mounting bracket 107, and mounting bracket 107 is gone up in adsorption piece 103's internally mounted has negative pressure generator 109, and mounting bracket 107's the other end fixedly connected with ejector pin 110, the upper end hinge of ejector pin 110 has draws material stick 111, draw and still be connected with extension spring 112 between material stick 111 and the ejector pin 110.
In this embodiment, a feeding cover plate 1011 is disposed at the upper end of the aseptic sampling tank 101, a sealing side plate 1012 is disposed at the side end of the aseptic sampling tank 101 corresponding to the adsorption member 103, a sample collecting tank 1016 is disposed at the bottom wall of the aseptic sampling tank 101, a protective isolation plate 1014 is obliquely disposed above the sample collecting tank 1016, and a material taking cover plate 1015 is disposed at the side end of the aseptic sampling tank 101 corresponding to the sample collecting tank 1016.
In this embodiment, the sliding track on the pushing portion 105 is of a varying width and has a circular ring shape in the middle.
In this embodiment, the adsorption member 103 is provided with ventilation micropores, and a side end surface of the adsorption member 103 is matched with the back of the sampling test piece 2.
In this embodiment, the sample collection well 1016 is vertically engaged with the cartridge 3.
Working process and principle: in the process of the invention, preparation before collection, test piece installation, multi-interval sampling, sampling acquisition and data analysis are sequentially carried out according to procedures, in the process of multi-interval sampling and sampling acquisition, a power device drives a transmission chain 4 to drive sampling equipment 1 to advance to a first interval to be collected, a negative pressure generator 109 is started to form suction force on the prismatic table-shaped end face of an adsorption piece 103, a steering engine 102 is started to drive an output end of the steering engine to drive a crank 104 to rotate clockwise (view angle of fig. 4), a pushing part 105 and a spring 108 drive a mounting frame 107 to synchronously move towards a sealing side plate 1012, a push rod 110 and a pulling rod 111 at the upper end are also driven to synchronously move in the process of moving the mounting frame 107, the end part of the pulling rod 111 keeps being abutted against a sampling test piece 2 at the uppermost end in a bearing box 3 under the action of the pulling force of a tension spring 112, the uppermost sampling test piece 2 is pulled out along with the movement of the pulling rod 111, and under the action of the suction force of the adsorption piece 103, the prismatic table-shaped end face of the adsorption piece 103 is stopped and adsorbed;
under the continuous advancing of the ejector rod 110, the end part of the ejector rod pushes the sealing side plate 1012 to open, the sampling test piece 2 attached to the prismatic table-shaped end surface of the adsorption piece 103 completely stretches out of the sterile sampling box 101, at the moment, the end part of the crank 104 is positioned in the large-diameter circular track in the middle of the pushing part 105, the steering engine 102 is stopped, the suspended and stationary sampling test piece 2 collects microbial flora from the horizontal, top down and bottom up, after the collection is finished, the steering engine 102 is started again to drive the adsorption piece 103 to return, the negative pressure generator 109 is stopped when the end part of the crank 104 slides to the large-diameter circular track in the other side of the middle of the pushing part 105, the sampling test piece 2 slides into the slide rod 106 below under the self gravity action, and under the blocking of the protective isolation plate 1014, the influence of the change of the sampling area from the external microbial flora is further avoided;
after the above operations are repeated to sequentially complete sample collection for all the processing regions, the material taking cover plate 1015 is opened to uniformly sample in the sample collecting slot 1016, and further data analysis is performed.
Based on the above, the invention sets the sampling device 1 on the transmission chain 4, the negative pressure generator 109 forms suction force on the prismatic table end face of the suction piece 103, after the pulled sampling test piece 2 is sucked, when the end part of the crank 104 is positioned in the large diameter circular track in the middle of the pushing part 105, the sampling test piece 2 in a suspended static state collects microorganism flora from the horizontal state, from top to bottom and from bottom to top, so as to effectively collect microorganism colony free in the horizontal state and in the flying state, and the accuracy of the microorganism colony image data obtained later is ensured;
along with the sampling of the sampling device 1 in different intervals driven by the transmission chain 4, after each sampling is completed, the sampling test piece 2 can stretch into the sterile sampling box 101 again and the sealing side plate 1012 is closed immediately, so that even the sampling device 1 shuttles in the atmosphere environment and the processing sampling interval can automatically collect data, and the microorganisms in the atmosphere environment are ensured not to diffuse into the processing interval, and the deviation of the collected data and the real data is further reduced.
It will be appreciated by those skilled in the art that the present invention can be carried out in other embodiments without departing from the spirit or essential characteristics thereof. Accordingly, the above disclosed embodiments are illustrative in all respects, and not exclusive. All changes that come within the scope of the invention or equivalents thereto are intended to be embraced therein.
Claims (4)
1. The data acquisition method based on the Internet of things is characterized by comprising the following steps of:
s1: preparation before acquisition: sterilizing the sampling equipment (1);
s2: and (3) mounting a test piece: filling a plurality of bacterial sampling test pieces (2) into a bearing box (3) in a sampling device in a sterile operation mode, wherein the sampling test pieces (2) are a prepared culture medium system, and comprise a standard culture medium, cold water soluble gel and an indicator;
s3: multi-region sampling: the terminal of the Internet of things sends a remote acquisition request, and the sampling equipment (1) is driven to sequentially travel to an interval to be acquired through a transmission chain (4), wherein the acquisition time is kept for 3-5min each time;
s4: sampling and obtaining: the sampling device (1) is reversely driven to return to the sampling position through the transmission chain (4);
s5: data analysis: the sampling device (1) comprises a sterile sampling box (101), a steering engine (102) and a prismatic adsorption piece (103), wherein the receiving sampling test piece (2) is acquired in the sampling device (1), colony image detection is carried out, colony data, corresponding microorganism growth environment and corresponding acquisition interval environment information are fed back and stored to an Internet of things terminal, the sampling device (1) comprises the sterile sampling box (101), the steering engine (102) and the prismatic adsorption piece (103), the receiving box (3) is installed in the sterile sampling box (101), the steering engine (102) is also installed in the sterile sampling box (101), the output end of the steering engine (102) is connected with a crank (104), the other end of the crank (104) is slidably provided with a pushing part (105), the pushing part (105) is slidably arranged in the sterile sampling box (101), the side end of the pushing part (105) is fixedly connected with two symmetrically arranged sliding rods (106), a mounting rack (107) is slidably arranged on the sliding rod (106), a spring (108) is sleeved between the mounting rack (107) and the pushing part (105), the adsorption piece (103) is installed on the sterile sampling box (101), the other end of the pushing rod (107) is slidably provided with a negative pressure generator (110), still be connected with extension spring (112) between draw material stick (111) and ejector pin (110), the upper end of aseptic sampling box (101) is provided with material loading apron (1011), and the side that corresponds with adsorption piece (103) on aseptic sampling box (101) is provided with sealed curb plate (1012), and the diapire of aseptic sampling box (101) is provided with collection appearance groove (1016), the top of collection appearance groove (1016) has put protection division board (1014) to one side, and the side that corresponds with collection appearance groove (1016) on aseptic sampling box (101) is provided with gets material apron (1015).
2. The data acquisition method based on the internet of things according to claim 1, wherein the method comprises the following steps: the sliding track on the pushing part (105) is of a width change and is of a circular ring shape in the middle.
3. The data acquisition method based on the internet of things according to claim 1, wherein the method comprises the following steps: the adsorption piece (103) is provided with ventilation micropores, and the side end face of the adsorption piece (103) is matched with the back of the sampling test piece (2).
4. The data acquisition method based on the internet of things according to claim 1, wherein the method comprises the following steps: the sample collection groove (1016) is matched with the containing box (3) in the vertical direction.
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| CN202210577880.3A CN114924042B (en) | 2022-05-25 | 2022-05-25 | Data acquisition method based on Internet of things |
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| CN202210577880.3A CN114924042B (en) | 2022-05-25 | 2022-05-25 | Data acquisition method based on Internet of things |
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| WO2008149636A1 (en) * | 2007-06-04 | 2008-12-11 | Kabushiki Kaisha N-Tech | Method for counting colonies |
| CN209338505U (en) * | 2018-11-28 | 2019-09-03 | 长春科技学院 | A kind of Microbiological detection of foods incubator |
| CN111964979A (en) * | 2020-08-27 | 2020-11-20 | 滁州学院 | A sampling device for hydrology information acquisition |
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