CN114924042A - Data acquisition method based on Internet of things - Google Patents
Data acquisition method based on Internet of things Download PDFInfo
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- CN114924042A CN114924042A CN202210577880.3A CN202210577880A CN114924042A CN 114924042 A CN114924042 A CN 114924042A CN 202210577880 A CN202210577880 A CN 202210577880A CN 114924042 A CN114924042 A CN 114924042A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0004—Gaseous mixtures, e.g. polluted air
- G01N33/0009—General constructional details of gas analysers, e.g. portable test equipment
- G01N33/0062—General constructional details of gas analysers, e.g. portable test equipment concerning the measuring method, e.g. intermittent, or the display, e.g. digital
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0004—Gaseous mixtures, e.g. polluted air
- G01N33/0009—General constructional details of gas analysers, e.g. portable test equipment
- G01N33/0027—General constructional details of gas analysers, e.g. portable test equipment concerning the detector
- G01N33/0036—Specially adapted to detect a particular component
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16Y—INFORMATION AND COMMUNICATION TECHNOLOGY SPECIALLY ADAPTED FOR THE INTERNET OF THINGS [IoT]
- G16Y40/00—IoT characterised by the purpose of the information processing
- G16Y40/10—Detection; Monitoring
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16Y—INFORMATION AND COMMUNICATION TECHNOLOGY SPECIALLY ADAPTED FOR THE INTERNET OF THINGS [IoT]
- G16Y40/00—IoT characterised by the purpose of the information processing
- G16Y40/20—Analytics; Diagnosis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P90/00—Enabling technologies with a potential contribution to greenhouse gas [GHG] emissions mitigation
- Y02P90/02—Total factory control, e.g. smart factories, flexible manufacturing systems [FMS] or integrated manufacturing systems [IMS]
Abstract
The invention discloses a data acquisition method based on the Internet of things, which relates to the technical field of data acquisition and comprises the following steps: the method comprises the steps of preparation before collection, test piece installation, multi-interval sampling, sampling acquisition and data analysis. According to the invention, the sampling device is arranged on the transmission chain, so that microbial colonies free from a horizontal state and a spray flying state can be effectively collected, and the accuracy of subsequently obtained microbial colony image data is ensured; along with the sampling of drive chain drive sampling equipment in different intervals, after sampling at every turn finishes, the sampling test piece all can stretch into aseptic sampling box once more and sealed curb plate closes immediately for even shuttle through the sampling equipment in atmospheric environment and processing sampling interval, also can the automatic acquisition data, and guarantee that atmospheric environment microorganism can not spread to the processing interval, further reduce the deviation of data collection and true data.
Description
Technical Field
The invention relates to the technical field of data acquisition, in particular to a data acquisition method based on the Internet of things.
Background
In the food and drug processing factory, the requirement on production and processing environment is high, the atmosphere environment in the production area needs to be regularly detected by microbial colonies, the variety is various, the process is complex, data acquisition needs to be carried out on a plurality of processing intervals, and in the prior art, the mode of respectively placing a bacteria sampling test piece is acquired through the specific acquisition position of an operator wearing a dust-free suit in each processing interval.
However, in the long-term data acquisition and measurement process in the prior art, certain disadvantages are found to exist: firstly, because the sampling test piece is directly and horizontally placed in the sampling interval, only the falling microbial community in the environment can be collected, and the microbial community which is free from the horizontal state and the spray flying state is not effectively collected, the obtained image data of the microbial community is inaccurate; two, a plurality of processing intervals are not necessarily in same closed region, and when crossing atmospheric environment, operating personnel still need frequent wearing to take off dustless clothes, and inefficiency just is difficult to guarantee that atmospheric environment microorganism can not spread to the processing interval for there is the deviation in data collection and true data.
Disclosure of Invention
The invention aims to provide a data acquisition method based on the Internet of things, so as to overcome the defects caused in the prior art.
A data acquisition method based on the Internet of things comprises the following steps:
s1: preparation before collection: sterilizing the sampling equipment;
s2: mounting a test piece: loading a plurality of bacteria sampling test pieces into a loading box in a sampling device in a sterile operation mode, wherein the sampling test pieces are a pre-prepared culture medium system and contain a standard culture medium, cold water soluble gel and an indicator;
s3: multi-interval sampling: the terminal of the Internet of things sends a remote acquisition request, the sampling equipment is driven to sequentially move to an interval to be acquired through a transmission chain, and the acquisition time is kept for 3-5min each time;
s4: sampling and obtaining: the sampling equipment is reversely driven to return to the sampling position through the transmission chain;
s5: and (3) data analysis: the sampling test piece that acquires in the sampling equipment is received, carries out bacterial colony image detection, bacterial colony data, the microorganism growth environment that corresponds and the environmental information feedback that corresponds the collection interval and store to thing networking terminal.
Preferably, sampling equipment includes aseptic sampling box, steering wheel and the absorption piece of prismoid shape, it installs in aseptic sampling box to hold the box, the steering wheel is also installed in aseptic sampling box, and the output of steering wheel is connected with the crank, the articulate other end slides and is provided with promotion portion, promotion portion slides and sets up in aseptic sampling box, the slide bar that two symmetries of side fixedly connected with of promotion portion set up, it is provided with the mounting bracket to slide on the slide bar, overlaps between mounting bracket and promotion portion on the slide bar to be equipped with the spring, adsorb the piece and install on the mounting bracket, have negative pressure generator in the internally mounted who adsorbs the piece on the mounting bracket, the other end fixedly connected with ejector pin of mounting bracket, the upper end of ejector pin articulates there is to draw the material stick, draw still be connected with the extension spring between material stick and the ejector pin.
Preferably, sampling equipment includes aseptic sampling box, steering wheel and the absorption piece of prismoid shape, it installs in aseptic sampling box to hold the box, the steering wheel is also installed in aseptic sampling box, and the output of steering wheel is connected with the crank, the articulate other end slides and is provided with promotion portion, promotion portion slides and sets up in aseptic sampling box, the slide bar that two symmetries of side fixedly connected with of promotion portion set up, it is provided with the mounting bracket to slide on the slide bar, overlaps between mounting bracket and promotion portion on the slide bar to be equipped with the spring, adsorb the piece and install on the mounting bracket, have negative pressure generator in the internally mounted who adsorbs the piece on the mounting bracket, the other end fixedly connected with ejector pin of mounting bracket, the upper end of ejector pin articulates there is the material stick of drawing, draw still be connected with the extension spring between material stick and the ejector pin.
Preferably, the sliding track on the pushing part is variable in width and annular in the middle.
Preferably, the adsorption piece is provided with a ventilating micropore, and the side end face of the adsorption piece is matched with the back of the sampling test piece.
Preferably, the sample collection groove is matched with the containing box in the vertical direction.
The invention has the advantages that:
(1) by arranging the sampling device on the transmission chain, the suction force is formed on the prismatic table-shaped end surface of the adsorption piece by the negative pressure generator, after the pulled sampling test piece is adsorbed, when the end part of the crank is positioned in the large-diameter circular track in the middle of the pushing part, the suspended sampling test piece in a static state collects microbial floras from the horizontal state, the top down state and the bottom up state, so that microbial colonies free from the horizontal state and the foam flying state can be effectively collected, and the accuracy of subsequently obtained microbial colony image data is ensured;
(2) along with the sampling of drive chain drive sampling equipment in different intervals, after sampling at every turn finishes, the sampling test piece all can stretch into aseptic sampling box once more and sealed curb plate closes immediately for even shuttle through the sampling equipment in atmospheric environment and processing sampling interval, also can the automatic acquisition data, and guarantee that atmospheric environment microorganism can not spread to the processing interval, further reduce the deviation of data collection and true data.
Drawings
FIG. 1 is a process flow diagram of the present invention.
FIG. 2 is a side view of the structure of the apparatus used in the present invention.
Fig. 3 is a schematic view of the sampling device of the present invention in an open state.
Fig. 4 is a schematic structural diagram of the sampling apparatus of the present invention.
Fig. 5 is an internal schematic view of a partial structure of the sampling apparatus of the present invention.
Fig. 6 is a partial structural schematic diagram of an aseptic sampling box according to the present invention.
The device comprises a sampling device 1, a sampling test piece 2, a containing box 3, a transmission chain 4, a sterile sampling box 101, a steering engine 102, an adsorption piece 103, a crank 104, a pushing part 105, a sliding rod 106, a mounting rack 107, a spring 108, a negative pressure generator 109, a top rod 110, a material pulling rod 111, a tension spring 112, a material feeding cover plate 1011, a sealing side plate 1012, a protecting isolation plate 1014, a material taking cover plate 1015 and a sample collecting groove 1016.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
As shown in fig. 1 to 6, a data acquisition method based on the internet of things includes the following steps:
s1: preparation before collection: sterilizing the sampling device 1;
s2: mounting a test piece: a plurality of bacteria sampling test pieces 2 are loaded into a loading box 3 in a sampling device in a sterile operation mode, and the sampling test pieces 2 are a pre-prepared culture medium system and contain a standard culture medium, cold water soluble gel and an indicator;
s3: multi-interval sampling: the terminal of the Internet of things sends a remote acquisition request, the sampling equipment 1 is driven to sequentially move to an interval to be acquired through the transmission chain 4, and the acquisition time is kept for 3-5min each time;
s4: sampling and obtaining: the sampling device 1 is reversely driven to return to the sampling position through the transmission chain 4 again;
s5: and (3) data analysis: receive the sampling test piece 2 that obtains in the sampling equipment 1, carry out bacterial colony image detection, bacterial colony data, the microorganism growth environment that corresponds and correspond the environmental information feedback of gathering the interval and store to thing networking terminal.
It should be noted that the sampling device 1 is suspended in the air, and the microorganism droplets can be captured in the horizontal direction and on the upper and lower sides of the sampling device 1.
In this embodiment, the sampling device 1 comprises a sterile sampling box 101, a steering engine 102 and a frustum-shaped adsorbing member 103, the box 3 is arranged in the sterile sampling box 101, the steering gear 102 is also arranged in the sterile sampling box 101, the output end of the steering gear 102 is connected with a crank 104, the other end of the crank 104 is provided with a pushing part 105 in a sliding manner, the pushing part 105 is arranged in the sterile sampling box 101 in a sliding manner, the side end of the pushing part 105 is fixedly connected with two symmetrically arranged sliding rods 106, a mounting rack 107 is slidably arranged on the slide bar 106, a spring 108 is sleeved on the slide bar 106 between the mounting rack 107 and the pushing part 105, the adsorption member 103 is mounted on a mounting frame 107, a negative pressure generator 109 is mounted inside the adsorption member 103 on the mounting frame 107, a top rod 110 is fixedly connected to the other end of the mounting frame 107, the upper end of the top rod 110 is hinged with a material pulling rod 111, and a tension spring 112 is connected between the material pulling rod 111 and the top rod 110.
In this embodiment, the upper end of the sterile sampling box 101 is provided with a feeding cover plate 1011, the side end of the sterile sampling box 101 corresponding to the adsorption part 103 is provided with a sealing side plate 1012, the bottom wall of the sterile sampling box 101 is provided with a sample collection groove 1016, a protection isolation plate 1014 is obliquely arranged above the sample collection groove 1016, and the side end of the sterile sampling box 101 corresponding to the sample collection groove 1016 is provided with a material taking cover plate 1015.
In this embodiment, the sliding track on the pushing portion 105 has a variable width and a circular ring shape in the middle.
In this embodiment, the adsorption member 103 is provided with air-permeable micropores, and a side end surface of the adsorption member 103 is fitted to the back of the sampling test strip 2.
In this embodiment, the sample well 1016 fits vertically with the cassette 3.
The working process and principle are as follows: in the using process of the invention, preparation before collection, test piece installation, multi-section sampling, sampling acquisition and data analysis are carried out in sequence according to working procedures, in the multi-section sampling and sampling acquisition process, firstly, a power device drives a transmission chain 4 to drive a sampling device 1 to move to a first section to be collected, a negative pressure generator 109 is started to form suction on a prismoid end face of an adsorption piece 103, a steering engine 102 is started to drive an output end of the steering engine to drive a crank 104 to rotate clockwise (viewing angle in figure 4), then a pushing part 105 and a spring 108 drive an installation frame 107 to synchronously move towards a sealing side plate 1012 direction, an ejector rod 110 and a material pulling rod 111 at the upper end are also driven to synchronously move in the moving process of the installation frame 107, under the pulling force of a tension spring 112, the end part of the material pulling rod 111 keeps attached to the uppermost sampling test piece 2 in a loading box 3, the uppermost sampling test piece 2 can be pulled out along with the movement of the material pulling rod 111, and the absorption piece 103 stops falling and absorbs on the prismatic table end surface of the absorption piece 103 under the action of the absorption force;
under the continuous advance of the push rod 110, the end part of the push rod pushes the sealing side plate 1012 to open, the sampling test piece 2 attached to the prismatic table-shaped end surface of the adsorption part 103 completely extends out of the sterile sampling box 101, at the moment, the end part of the crank 104 is positioned in a large-diameter circular track in the middle of the pushing part 105, the steering engine 102 is turned off, the sampling test piece 2 in a suspended static state collects microbial flora from the horizontal direction, the top-down direction and the bottom-up direction, after the collection is finished, the steering engine 102 is started again, the output end of the steering engine drives the adsorption part 103 to return, when the end part of the crank 104 slides to the large-diameter circular track on the other side in the middle of the pushing part 105, the negative pressure generator 109 is turned off, the sampling test piece 2 slides into the sliding rod 106 below under the self gravity of the sampling test piece, and under the blocking of the protective isolation plate 1014, the influence of the sampling interval from external microbial flora is further avoided;
after the above operations are repeated to complete the sample collection of all the processing sections in sequence, the material taking cover plate 1015 is opened to uniformly sample in the sample collecting groove 1016, and further data analysis is performed.
Based on the above, in the present invention, by arranging the sampling apparatus 1 on the transmission chain 4, the negative pressure generator 109 forms a suction force on the prismoid end surface of the adsorption member 103, and after adsorbing the pulled-down sampling test piece 2, when the end of the crank 104 is located in the large-diameter circular track in the middle of the pushing portion 105, the suspended and static sampling test piece 2 collects microbial flora from horizontal, top-down and bottom-up states, so as to effectively collect microbial colonies free from the horizontal state and flying foam, and most ensure the accuracy of subsequently obtained microbial colony image data;
along with the sampling of drive chain 4 drive sampling equipment 1 in different intervals, after sampling at every turn finishes, sampling test piece 2 all can stretch into aseptic sampling box 101 once more and sealed curb plate 1012 closes immediately for even shuttle through the sampling equipment 1 in atmospheric environment and processing sampling interval, also can automatic data acquisition, and guarantee that atmospheric environment microorganism can not spread to the processing interval, further reduce the deviation of data acquisition and true data.
It will be appreciated by those skilled in the art that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The embodiments disclosed above are therefore to be considered in all respects as illustrative and not restrictive. All changes which come within the scope of or equivalence to the invention are intended to be embraced therein.
Claims (6)
1. A data acquisition method based on the Internet of things is characterized by comprising the following steps:
s1: preparation before collection: sterilizing the sampling device (1);
s2: mounting a test piece: a plurality of bacteria sampling test pieces (2) are arranged in a containing box (3) of a sampling device in a sterile operation mode, and the sampling test pieces (2) are a pre-prepared culture medium system and contain a standard culture medium, cold water soluble gel and an indicator;
s3: multi-interval sampling: the terminal of the Internet of things sends a remote acquisition request, the sampling equipment (1) is driven to sequentially advance to a section to be acquired through a transmission chain (4), and the acquisition time is kept for 3-5min each time;
s4: sampling and obtaining: the sampling device (1) is reversely driven to return to a sampling position through the transmission chain (4);
s5: and (3) data analysis: receive sampling test piece (2) of acquireing in the sampling equipment (1), carry out the bacterial colony image detection, bacterial colony data, the microorganism growth environment that corresponds and the environmental information feedback that corresponds the collection interval and store to thing networking terminal.
2. The data acquisition method based on the internet of things as claimed in claim 1, wherein: the sampling device (1) comprises a sterile sampling box (101), a steering engine (102) and a frustum-shaped adsorption piece (103), the bearing box (3) is installed in the sterile sampling box (101), the steering engine (102) is also installed in the sterile sampling box (101), the output end of the steering engine (102) is connected with a crank (104), the other end of the crank (104) is provided with a pushing part (105) in a sliding manner, the pushing part (105) is arranged in the sterile sampling box (101) in a sliding manner, two symmetrically arranged sliding rods (106) are fixedly connected to the side end of the pushing part (105), an installation frame (107) is arranged on the sliding rods (106) in a sliding manner, a spring (108) is sleeved between the installation frame (107) and the pushing part (105) on the sliding rods (106), the adsorption piece (103) is installed on the installation frame (107), and a negative pressure generator (109) is installed inside the adsorption piece (103) on the installation frame (107), the other end fixedly connected with ejector pin (110) of mounting bracket (107), the upper end of ejector pin (110) articulates there is and draws material stick (111), draw and still be connected with extension spring (112) between material stick (111) and ejector pin (110).
3. The data acquisition method based on the internet of things as claimed in claim 2, wherein: the upper end of the sterile sampling box (101) is provided with a feeding cover plate (1011), the side end, corresponding to the adsorption part (103), of the sterile sampling box (101) is provided with a sealing side plate (1012), the bottom wall of the sterile sampling box (101) is provided with a sample collection groove (1016), a protective isolation plate (1014) is obliquely arranged above the sample collection groove (1016), and the side end, corresponding to the sample collection groove (1016), of the sterile sampling box (101) is provided with a material taking cover plate (1015).
4. The data acquisition method based on the internet of things as claimed in claim 2, wherein: the sliding track on the pushing part (105) is variable in width and is circular in the middle.
5. The data acquisition method based on the internet of things as claimed in claim 2, wherein: the adsorption piece (103) is provided with a ventilating micropore, and the side end face of the adsorption piece (103) is matched with the back of the sampling test piece (2).
6. The data acquisition method based on the Internet of things as claimed in claim 3, wherein: the sample collecting groove (1016) is matched with the bearing box (3) in the vertical direction.
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| MUMTAZ R等: "Internet of things (IoT) based indoor air quality sensing and predictive analytic—A COVID-19 perspective" * |
| 中华人民共和国国家质量监督检验检疫总局: "食品中菌落总数的测定 PetrilfilmTM测试片法" * |
| 陈浩: "分析微生物检验标本不合格原因分析及质量控制对策" * |
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