CN114917298B

CN114917298B - Pharmaceutical composition for treating pneumonia and preparation method thereof

Info

Publication number: CN114917298B
Application number: CN202210492157.5A
Authority: CN
Inventors: 金明月; 李春梅; 王凤环
Original assignee: Sunlon Pharmaceutical Co ltd
Current assignee: Sunlon Pharmaceutical Co ltd
Priority date: 2022-05-07
Filing date: 2022-05-07
Publication date: 2023-10-20
Anticipated expiration: 2042-05-07
Also published as: CN114917298A

Abstract

The invention relates to the technical field of traditional Chinese medicines, in particular to a pharmaceutical composition for treating pneumonia and a preparation method thereof. The pharmaceutical composition comprises the following components in parts by weight: 10-15 parts of fritillaria cirrhosa, 2-10 parts of ephedra, 10-20 parts of loquat leaf, 5-10 parts of peppermint, 5-10 parts of weeping forsythiae capsule, 2-5 parts of poria cocos, 2-5 parts of cordate houttuynia, 10-15 parts of honeysuckle, 2-5 parts of baical skullcap root, 1-5 parts of cape jasmine, 5-15 parts of Chinese thorowax root, 2-10 parts of white mulberry root-bark, 5-10 parts of pilose asiabell root and 2-5 parts of oyster. The preparation method comprises the following steps: soaking the raw materials, decocting and filtering to obtain filtrate and filter residue; reflux-extracting filter residue with ethanol water solution; adding beta-cyclodextrin into the filtrate, stirring uniformly, and drying; and adding the extracting solution, cellulose and beta-cyclodextrin, uniformly mixing, drying and granulating. The medicine has effects of replenishing blood, nourishing blood, and enhancing immunity. Can not only treat pneumonia, but also strengthen physique and treat both symptoms and root causes.

Description

Pharmaceutical composition for treating pneumonia and preparation method thereof

Technical Field

The invention relates to the technical field of traditional Chinese medicines, in particular to a pharmaceutical composition for treating pneumonia and a preparation method thereof.

Background

Pneumonia is a common respiratory disease and is a substantial inflammation of the lungs caused by a variety of pathogens (e.g., bacteria, viruses, fungi, parasites, etc.). The main symptoms are high fever, very rapid breathing and persistent dry cough, which may have unilateral chest pain, chest pain during deep breathing and cough, small or large amount of phlegm, and blood streak.

At present, most Western medicines are anti-inflammatory medicines for treating pneumonia, which take effect quickly, but most anti-inflammatory medicines are antibiotics, and the anti-inflammatory medicines take effect quickly, but are easy to generate drug resistance, and the medicines are easy to relapse after stopping taking the medicines, so that the symptoms are treated without treating the root causes.

Pneumonia belongs to the categories of wind-warm syndrome, cough, lung heat disease and the like in traditional Chinese medicine. Traditional Chinese medicine considers that pneumonia is caused by overstrain, wind-heat or pathogenic wind-cold entering into interior to transform heat when body's vital qi is insufficient after drunk. The Chinese medicine is suitable for dispelling wind, dispersing obstruction, eliminating phlegm, relieving asthma, clearing away heat and toxic material, promoting the production of body fluid and quenching thirst. Flos Lonicerae, fructus forsythiae, scutellariae radix, radix Isatidis, and flos Caryophylli can be added into Ma XING SHI GAN TANG. Modified Sanhuang Gao Tang (decoction of Sanhuang Gao for serious disease). For yin impairment due to heat, vexation and shortness of breath, shengmai san or SHASHEN radix Ophiopogonis decoction can be added.

The traditional Chinese medicine has small side effect for treating pneumonia, treats both symptoms and root causes, but the traditional Chinese medicine formula is generally complex, has the defects of long treatment course, slow effect and the like, is mostly decoction, and is relatively troublesome to take.

Disclosure of Invention

The invention aims to provide a pharmaceutical composition for treating pneumonia, which utilizes the synergistic effect of a plurality of traditional Chinese medicinal materials to improve the curative effect of the medicine, has the effects of clearing heat and detoxicating, eliminating phlegm and relieving asthma, promoting the production of body fluid and quenching thirst, and has the advantages of small side effect, short treatment course and remarkable curative effect.

The invention also aims to provide a preparation method of the pharmaceutical composition for treating pneumonia, which comprises the steps of extracting water-soluble components in traditional Chinese medicinal materials through soaking and boiling, and extracting active ingredients in dregs by using ethanol water solution in a reflux way, wherein the active ingredients in the traditional Chinese medicinal materials are fully utilized, and the content of the active ingredients in the pharmaceutical composition is high so as to improve the curative effect of the medicine.

The invention solves the technical problems by adopting the following technical scheme.

In one aspect, the embodiment of the invention provides a pharmaceutical composition for treating pneumonia, which comprises the following components in parts by weight: 10-15 parts of fritillaria cirrhosa, 2-10 parts of ephedra, 10-20 parts of loquat leaf, 5-10 parts of peppermint, 5-10 parts of weeping forsythiae capsule, 2-5 parts of poria cocos, 2-5 parts of cordate houttuynia, 10-15 parts of honeysuckle, 2-5 parts of baical skullcap root, 1-5 parts of cape jasmine, 5-15 parts of Chinese thorowax root, 2-10 parts of white mulberry root-bark, 5-10 parts of pilose asiabell root and 2-5 parts of oyster.

On the other hand, the embodiment of the invention provides a preparation method of a pharmaceutical composition for treating pneumonia, which comprises the steps of S1, soaking raw materials in a solvent, then decocting for 5-6h at 90-110 ℃, and filtering to obtain filtrate and filter residues;

s2, crushing filter residues, and adding an ethanol aqueous solution for reflux extraction to obtain an extracting solution;

s3, adding a part of beta-cyclodextrin into the filtrate, uniformly stirring, and drying to obtain a mixture;

s4, uniformly mixing the mixture obtained in the step S3, the extracting solution, cellulose and the other part of beta-cyclodextrin, drying and granulating.

Compared with the prior art, the embodiment of the invention has at least the following advantages or beneficial effects:

in the invention, the Chinese medicinal materials such as the fritillaria cirrhosa, the loquat leaf, the bupleurum and the like are used together, so that the Chinese medicinal composition has the effects of resisting bacteria and diminishing inflammation, clearing away heat and toxic materials, eliminating phlegm and relieving asthma, promoting the production of body fluid and quenching thirst, and has the advantages of small side effect, short treatment course and remarkable curative effect. The traditional Chinese medicines such as tuckahoe, codonopsis pilosula and the like are added and are compatible with the fritillary bulb, the loquat leaf and the like, so that the medicine has the effects of enriching and nourishing blood, strengthening physique and improving body immunity. Can not only treat pneumonia, but also strengthen physique and treat both symptoms and root causes.

When the medicine is prepared, the effective components of the traditional Chinese medicinal materials are fully utilized by water extraction and then alcohol extraction, so that the content of the effective components in the medicine composition is high, and the curative effect of the medicine is improved. Compared with the traditional Chinese medicine decoction, the traditional Chinese medicine composition has the advantages of richer effective components and better curative effect by alcohol extraction.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.

It should be noted that, without conflict, the embodiments of the present invention and features of the embodiments may be combined with each other. The present invention will be described in detail with reference to specific examples.

Bulbus Fritillariae Cirrhosae has effects of relieving cough, eliminating phlegm, moistening lung, and resolving hard mass. It is used for treating cough due to lung deficiency, cough due to consumptive disease, cough due to dryness-heat, lung abscess, scrofula, carbuncle, swelling, and acute mastitis.

Herba Ephedrae has effects of inducing sweat, relieving exterior syndrome, dispersing lung qi, relieving asthma, inducing diuresis and relieving swelling. Is mainly used for treating wind-cold exterior excess syndrome, cough and asthma, wind and water, difficult urination, wind-damp arthralgia, skin irritability, rubella and pruritus, yin gangrene and phlegm nodule.

Loquat leaf has the effects of clearing lung-heat, relieving cough, harmonizing stomach, descending adverse qi and quenching thirst. Is used for treating cough due to lung heat and phlegm, cough due to yin deficiency, hemoptysis, epistaxis, hematemesis, vomiting due to stomach heat, vomiting of pregnancy, infantile vomiting, diabetes and facial sore due to lung wind.

The herba Menthae has effects of dispelling pathogenic wind heat, clearing head and eyes, relieving sore throat, promoting eruption, dispersing stagnated liver qi, and activating qi-flowing, and can be used for treating diseases such as wind heat, headache, laryngopharynx swelling and pain, food stagnation, flatulence, aphtha, toothache, sore, scabies, urticaria, epidemic febrile at the beginning of epidemic febrile disease, pruritis, liver depression, qi stagnation, chest distress, hypochondriac pain, etc.

Fructus forsythiae has effects of clearing heat, removing toxic substances, resolving hard mass, and detumescence. It is indicated for warm heat, erysipelas, macula, carbuncle, abscess, scrofula, stranguria with urine.

Poria is light in smell and oozes, ascending its nature, producing body fluids, opening the striae and skin pores, nourishing water and descending, and promoting urination. Has effects of promoting diuresis, removing dampness, invigorating spleen, and calming heart. Can be used for treating edema, oliguria, phlegm retention, palpitation, spleen deficiency, diarrhea, heart mind disorder, palpitation, and insomnia.

The herba Houttuyniae is dry aerial part of houttuynia cordata Thunb of Saururaceae. Yu xing Cao is pungent in flavor, cold in nature and cool in nature, and enters lung meridian. Has effects in clearing away heat and toxic materials, relieving swelling, treating sore, promoting urination, removing dampness, clearing heat, relieving dysentery, invigorating stomach, and resolving food stagnation, and can be used for treating lung abscess, pyocutaneous disease, hematochezia, spleen and stomach heat caused by excessive heat, toxic heat, damp evil, and disease heat.

The honeysuckle has the effects of clearing heat and detoxicating. Is mainly used for treating exogenous wind-heat or warm disease fever, heatstroke, toxic heat blood dysentery, carbuncle, furuncle, pharyngitis and various infectious diseases.

The radix Scutellariae has effects of clearing heat, eliminating dampness, purging pathogenic fire, removing toxic substances, stopping bleeding, and preventing miscarriage, and can be used for treating damp-heat, summer-heat dampness, chest distress, emesis, damp-heat distention and fullness, dysentery, jaundice, cough due to lung heat, polydipsia due to high fever, hematemesis, carbuncle, skin sore, and fetal movement.

Fructus Gardeniae is fruit of Gardenia jasminoides Ellis of Rubiaceae. Has effects in protecting liver, promoting function of gallbladder, lowering blood pressure, tranquilizing, stopping bleeding, and relieving swelling. It is used for treating icteric hepatitis, sprain and contusion, hypertension, and diabetes.

Bupleuri radix has effects of relieving exterior and interior syndrome, dispersing stagnated liver qi and invigorating yang. Can be used for treating common cold, fever, cold and heat, malaria, liver depression, qi stagnation, chest pain, rectocele, uterine prolapse, and menoxenia.

The mulberry bark is dry root bark of dicotyledon Moraceae mulberry with the plug removed, has sweet and cold nature, enters lung and spleen channels, has the effects of purging lung and relieving asthma, and inducing diuresis and detumescence, and is mainly used for treating lung heat asthma and cough, oliguria edema, swelling of face skin and the like. It also has antihypertensive, antiinflammatory, and anticancer effects.

Dang Shen is sweet in flavor and neutral in nature. Has effects in invigorating spleen, replenishing qi, quenching thirst, invigorating spleen, tonifying lung, nourishing blood, and promoting salivation. Can be used for treating spleen and lung qi deficiency, anorexia, cough, asthma, deficiency of qi and blood, sallow complexion, palpitation, short breath, thirst due to body fluid deficiency, and internal heat and diabetes. Lazy speaking, weak limbs, poor appetite, qi deficiency, deficiency of both qi and fluid, deficiency of both qi and blood, and chlorosis due to blood deficiency.

Oyster is shell of oyster family animal. The main functions are as follows: astringing yin, suppressing yang, suppressing sweating, astringing essence, eliminating phlegm, and softening hard masses. It is indicated for convulsive epilepsy, dizziness, spontaneous perspiration, night sweat, spermatorrhea, stranguria with turbid urine, metrorrhagia, leukorrhagia, scrofula and goiter. Can be used for treating palpitation, insomnia, dizziness, tinnitus, scrofula, phlegm nodule, spontaneous perspiration, night sweat, spermatorrhea, leukorrhagia, gastralgia, and pantothenic acid.

In the invention, the bulbus fritillariae cirrhosae, the ephedra and the loquat leaf are compatible, have the effects of relieving cough and reducing sputum, freeing lung and relieving asthma, clearing lung and relieving cough, and are mainly used for treating symptoms such as asthma, cough and the like caused by pneumonia. Herba Menthae, fructus forsythiae, herba Houttuyniae, flos Lonicerae, and radix astragali have effects of clearing heat and detoxicating, removing turbid qi in lung, removing lung toxic substances, and can be used for treating lung inflammation in cooperation with Bulbus Fritillariae Cirrhosae. Radix Codonopsis and Poria have effects of promoting diuresis, removing dampness, invigorating spleen, calming heart, invigorating spleen and replenishing qi, quenching thirst, invigorating spleen and replenishing lung qi, improving qi and blood, strengthening body constitution, enhancing immunity, and reducing virus invasion.

In some embodiments of the present invention, the above-mentioned Chinese medicine composition further comprises 5-6 parts of chrysanthemum and 4-5 parts of licorice.

Chrysanthemum enters lung and liver meridians. Has effects of dispelling pathogenic wind, clearing heat, suppressing hyperactive liver, improving eyesight, and clearing heat and toxic materials.

Licorice belongs to perennial herb, the root and the rhizome are thick and strong, and the licorice is a tonic Chinese herbal medicine. The medicinal parts are roots and rhizomes, the medicinal material characteristic roots are cylindrical, the length is 25-100 cm, and the diameter is 0.6-3.5 cm. The skin has different tightness, and the surface is reddish brown or grey brown. The rhizome is cylindrical, the surface is provided with bud marks, and the middle part of the section is provided with marrow. Slight smell, sweet and special taste. The functions of the medicine are mainly clearing heat and detoxicating, eliminating phlegm and relieving cough, and the like.

In the formula, chrysanthemum and liquorice are added, and the effects of dispelling wind, clearing heat, calming liver, improving eyesight, clearing heat and detoxicating of the chrysanthemum are utilized, and the effects of clearing heat and detoxicating of the traditional Chinese medicine composition are further improved by matching with traditional Chinese medicines such as peppermint; the effects of clearing heat and detoxicating, eliminating phlegm and relieving cough of the liquorice are utilized, and Chinese medicinal materials such as peppermint, fritillaria cirrhosa and the like are matched, so that lung heat is removed, phlegm and relieving cough are eliminated, and the treatment effect is further improved.

In some embodiments of the present invention, the above-mentioned Chinese medicine components further comprise 5-6 parts of Chinese angelica, 2-5 parts of ligusticum chuanxiong and 2-10 parts of radix sileris.

Radix Angelicae sinensis has effects of replenishing blood, promoting blood circulation, regulating menstruation, relieving pain, moistening dryness, and smoothing intestine.

The ligusticum chuanxiong hort has the functions of promoting qi circulation, resolving depression, dispelling wind, eliminating dampness, promoting blood circulation and relieving pain. It is used to treat headache, dizziness, hypochondrium pain, abdominal pain, cold arthralgia, spasm of tendons, headache, rheumatalgia, amenorrhea, dystocia, puerperal stagnation, pain, carbuncle, and ulcer.

Radix Saposhnikoviae has effects of dispelling pathogenic wind, relieving exterior syndrome, eliminating dampness, relieving pain, and relieving spasm. Is used for treating exterior syndrome, rubella pruritus, rheumatalgia, tetanus, spleen deficiency and excessive dampness.

The Chinese angelica is added in the formula, and the Chinese angelica is used for replenishing qi and blood for the organism by utilizing the blood replenishing and activating effects of the Chinese angelica, is matched with Chinese medicinal materials such as dangshen and the like, and has the effects of strengthening physique and improving organism immunity. Adding rhizoma Ligustici Chuanxiong, and using rhizoma Ligustici Chuanxiong for promoting circulation of qi, resolving stagnation, dispelling pathogenic wind, removing dampness, promoting blood circulation, relieving pain, and improving immunity by matching with radix Angelicae sinensis and radix Codonopsis, promoting blood circulation, replenishing blood, invigorating spleen and replenishing qi.

In some embodiments of the present invention, the above-mentioned traditional Chinese medicine components further comprise 2-5 parts of katsumada, 5-10 parts of dried orange peel and 2-5 parts of dendrobium candidum.

Rock Ma Sangke has effects of clearing heat and detoxicating, dispelling pathogenic wind and cold, and can be used for treating common cold, fever and cough caused by wind and cold. The pericarpium Citri Tangerinae has effects of regulating qi-flowing, invigorating spleen, eliminating dampness and eliminating phlegm.

Dendrobium officinale has the effects of promoting fluid production, nourishing stomach, nourishing yin, clearing heat, moistening lung, tonifying kidney, improving eyesight and strengthening waist. Dendrobium officinale has the effect of promoting fluid production, and is mainly used for promoting gland secretion and organ movement. Dendrobium officinale can promote phagocytic function of tumor-bearing animal macrophages, enhance proliferation and differentiation of T lymphocytes and activity of NK cells, and can obviously improve serum hemolysin value of tumor-bearing animals, so that TPSH has no matter of nonspecific immunity or specific cellular immunity and humoral immunity, and can improve body immunity.

By adding the yanmasang, the Chinese medicinal composition is compatible with chrysanthemum, peppermint and the like for use, and has the effects of clearing heat, detoxicating and dispelling wind-cold; the dendrobium candidum is added and is compatible with dangshen and angelica for use, so that the immunity of the organism is improved.

In some embodiments of the present invention, the above-mentioned traditional Chinese medicine component further comprises 5-10 parts of beta-cyclodextrin and 5-10 parts of cellulose. The effective components of the traditional Chinese medicine are packed into particles by taking beta-cyclodextrin and cellulose as packing wall materials, so that the food is convenient to eat.

s4, uniformly mixing the mixture, the extracting solution, cellulose and the other part of beta-cyclodextrin, drying and granulating.

In some embodiments of the invention, in the step S1, the solvent comprises cellulase, hemicellulase, pectinase and water. Before water extraction, biological enzyme is added into the soaked solvent, and enzymolysis is performed by using the biological enzyme to destroy cellulose and the like of the traditional Chinese medicinal materials, so that the dissolution of the effective components of the traditional Chinese medicinal materials is quickened, and the water extraction efficiency is improved.

In some embodiments of the invention, the cellulase, the hemicellulase, and the pectinase are in a mass ratio of 1: (0.5-1): (0.2-0.5). The traditional Chinese medicinal materials have high cellulose content, the cellulase is used as a main component, the enzymolysis efficiency can be improved, the hemicellulase and the pectase are used as auxiliary components, and the mixture ratio of the hemicelluloses and the pectase is reasonable, so that three cellulases can be fully utilized, and the comprehensive enzymolysis efficiency is high.

In some embodiments of the present invention, in the step S2, the crushed filter residue has a particle size of 200-300 mesh, and the ethanol aqueous solution has a volume fraction of 50-60%. The grain size of the crushed dregs becomes smaller, the contact area between the dregs and the ethanol water solution is large, and the effective components are more easily dissolved out.

In some embodiments of the invention, the mass ratio of the β -cyclodextrin of step S3 to the β -cyclodextrin of step S4 is 1: (1-2).

The features and capabilities of the present invention are described in further detail below in connection with the examples.

Example 1

Preparing 10Kg of fritillaria cirrhosa, 5Kg of ephedra, 10Kg of loquat leaf, 5Kg of mint, 5Kg of weeping forsythia, 2Kg of poria cocos, 5Kg of houttuynia cordata, 10Kg of honeysuckle, 2Kg of scutellaria baicalensis, 1Kg of gardenia, 10Kg of radix bupleuri, 2Kg of white mulberry root-bark, 5Kg of codonopsis pilosula and 2Kg of oyster.

The pharmaceutical composition is prepared as follows:

s1, adding 1Kg of cellulase, 0.5Kg of hemicellulase and 0.5Kg of pectase into 40Kg of water, uniformly mixing to obtain a soaking solvent, soaking the traditional Chinese medicinal materials in the soaking solvent for 48 hours, stirring every 2 hours for 10 minutes, and ensuring that the traditional Chinese medicinal materials are completely soaked in the solvent. And then decocting the traditional Chinese medicine materials and the soaking solvent for 6 hours at 100+/-2 ℃, filtering, respectively collecting filtrate and filter residues, and concentrating the water content of the filtrate to be 10wt%.

S2, crushing filter residues in a crusher, sieving with a 300-mesh sieve, adding an ethanol water solution with the volume fraction of 50%, carrying out reflux extraction for 10 hours in a reflux extraction device, collecting an extracting solution, and concentrating until the water content is 10wt%.

S3, adding 2Kg of beta-cyclodextrin into the concentrated filtrate, uniformly stirring, and drying until the water content is 2wt%.

S4, taking 2Kg of beta-cyclodextrin, 1Kg of cellulose and the dried mixture in the step S3, adding the dried mixture into the concentrated extracting solution, uniformly mixing, spraying, granulating and drying to obtain the pharmaceutical composition.

Example 2

15Kg of fritillaria cirrhosa, 2Kg of ephedra, 15Kg of loquat leaf, 8Kg of mint, 8Kg of weeping forsythia, 5Kg of poria cocos, 2Kg of houttuynia cordata, 15Kg of honeysuckle, 3Kg of scutellaria baicalensis, 5Kg of gardenia, 5Kg of bupleurum, 5Kg of white mulberry root-bark, 10Kg of codonopsis pilosula and 5Kg of oyster are prepared.

The pharmaceutical composition is prepared as follows:

s1, adding 1Kg of cellulase, 1Kg of hemicellulase and 0.2Kg of pectase into 40Kg of water, uniformly mixing to obtain a soaking solvent, soaking the traditional Chinese medicinal materials in the soaking solvent for 48 hours, stirring every 2 hours for 10 minutes, and ensuring that the traditional Chinese medicinal materials are completely soaked in the solvent. And then decocting the traditional Chinese medicine materials and the soaking solvent for 6 hours at 90+/-2 ℃, filtering, respectively collecting filtrate and filter residues, and concentrating the water content of the filtrate to be 10wt%.

S2, crushing filter residues in a crusher, sieving with a 300-mesh sieve, adding an ethanol water solution with the volume fraction of 60%, carrying out reflux extraction for 10 hours in a reflux extraction device, collecting an extracting solution, and concentrating until the water content is 10wt%.

S3, adding 1Kg of beta-cyclodextrin into the concentrated filtrate, uniformly stirring, and drying until the water content is 2wt%.

S4, adding 2Kg of beta-cyclodextrin, 0.5Kg of cellulose and the dried mixture obtained in the step S3 into the concentrated extracting solution, uniformly mixing, spraying, granulating and drying to obtain the pharmaceutical composition.

Example 3

12Kg of fritillaria cirrhosa, 10Kg of ephedra, 20Kg of loquat leaf, 10Kg of mint, 10Kg of weeping forsythia, 3Kg of poria cocos, 3Kg of houttuynia cordata, 12Kg of honeysuckle, 5Kg of scutellaria baicalensis, 3Kg of gardenia, 10Kg of radix bupleuri, 10Kg of white mulberry root-bark, 8Kg of codonopsis pilosula and 3Kg of oyster are prepared.

The pharmaceutical composition is prepared as follows:

s1, adding 1Kg of cellulase, 0.8Kg of hemicellulase and 0.3Kg of pectase into 40Kg of water, uniformly mixing to obtain a soaking solvent, soaking the traditional Chinese medicinal materials in the soaking solvent for 48 hours, stirring every 2 hours for 10 minutes, and ensuring that the traditional Chinese medicinal materials are completely soaked in the solvent. And then decocting the traditional Chinese medicine materials and the soaking solvent at 110+/-2 ℃ for 6 hours, filtering, respectively collecting filtrate and filter residues, and concentrating the water content of the filtrate to be 10wt%.

S2, crushing filter residues in a crusher, sieving with a 200-mesh sieve, adding an ethanol water solution with the volume fraction of 50%, carrying out reflux extraction for 10 hours in a reflux extraction device, collecting an extracting solution, and concentrating until the water content is 10wt%.

S4, adding 3Kg of beta-cyclodextrin, 1Kg of cellulose and the dried mixture obtained in the step S3 into the concentrated extracting solution, uniformly mixing, spraying, granulating and drying to obtain the pharmaceutical composition.

Example 4

On the basis of the example 1, 5Kg of chrysanthemum and 4Kg of liquorice are also added. The remainder was the same as in example 1.

Example 5

On the basis of the example 1, 6Kg of chrysanthemum and 4.5Kg of liquorice are also added. The remainder was the same as in example 1.

Example 6

On the basis of the example 1, 5.5Kg of chrysanthemum and 5Kg of liquorice are also added. The remainder was the same as in example 1.

Example 7

On the basis of example 4, 5Kg of angelica sinensis, 3Kg of ligusticum chuanxiong and 10Kg of radix sileris are also added. The remainder was the same as in example 4.

Example 8

On the basis of example 4, 6Kg of angelica sinensis, 2Kg of ligusticum chuanxiong and 8Kg of radix sileris are also added. The remainder was the same as in example 4.

Example 9

On the basis of example 4, 5.5Kg of angelica sinensis, 5Kg of ligusticum chuanxiong and 2Kg of radix sileris are also added. The remainder was the same as in example 4.

Example 10

On the basis of example 7, 5Kg of katsumade mulberry, 6Kg of dried orange peel and 3Kg of dendrobium candidum are also added. The remainder was the same as in example 7.

Example 11

Based on the example 7, 3.5Kg of katsumade mulberry, 10Kg of dried orange peel and 5Kg of dendrobium candidum are also added. The remainder was the same as in example 7.

Example 12

Based on the example 7, 2Kg of katsumade mulberry, 5Kg of dried orange peel and 2Kg of dendrobium candidum are also added. The remainder was the same as in example 7.

Experimental example

1. Construction of a mouse pneumonia model

60 normal mice are selected and divided into 6 groups, 10 mice in each group are half of the normal mice, and the normal mice are adaptively fed for 2 days and then inoculated with viruses. On day 1, influenza virus was inoculated, normal mice were instilled with physiological saline in the nasal cavity, and the remaining mice were instilled with 20 μl of influenza a H1N1 virus FM1 strain.

2. After 1 day of virus inoculation, the experimental group was fed with the drugs of example 1, example 4, example 7 and example 10, respectively, 2 times per day, 1g each time; normal and model mice were perfused with the same amount of physiological saline and administered continuously to day 14.

The systemic response, including hair, activity status, defecation, ingestion, respiration, etc., of each group of mice after virus infection was observed daily, and the daily body mass and death of the mice were recorded.

Normal mice have good mental state and respond sharply to the external environment. After 2d of infection of mice in the model group with virus, the conditions of diet reduction and urine volume reduction gradually appear, mao Songyi falls off, shortness of breath, sleepiness, cluster and curl, aversion to cold and tremble, stiff movement and listlessness. The states of the mice in each experimental group are shown in table 1.

Table 1 mice status of experimental group

As can be seen from the above Table 1, the mice with pneumonia were gradually improved in mental state after taking the medicines of the examples, and after taking the medicines for 14 days, the mice of each experimental group were good in mental state, and the medicines of the examples had a certain therapeutic effect on the pneumonia of the mice.

On day 14 of drug infusion, mice were sacrificed, the mass of the mice was weighed, the whole lung was removed, and then the whole lung was washed with physiological saline to suck out surface water, the mass of the lung was weighed, the lung index of each group of mice was measured by calculating the lung index, and the lung index, the lung inhibition ratio was measured, wherein the lung index= (lung mass/mouse mass) ×100%, the lung index inhibition ratio= [ model group average lung index-experimental group average lung index ]/[ model group average lung index-blank group average lung index ] ×100%, and the lung index inhibition ratio of each group of mice was as shown in table 2. And after day 14, the mortality of each group of mice was counted, and the results are shown in Table 2

Table 2 lung index of mice in each group

Example 1

Example 4

Example 7

Example 10

Model group

Normal group

Lung index inhibition rate

25.3％

25.1％

24.6％

24.3％

/

Mortality rate of

0

90％

0

From the above table 2, it can be obtained that the drug of the embodiment of the invention has a specific effect of treating pneumonia with an inhibition rate of 24-25% on lung index. And none of the mice in the experimental group died during the 14 days of treatment, whereas the mortality rate in the model group was 90%.

3 clinical trials

The treatment effect was counted by selecting 40 patients with pneumonia, randomly dividing them into 4 groups, and taking the medicines of example 1, example 4, example 7 and example 10, 3g each time, 2 times daily, and 3 months each time, each group of 10 patients with the clinical diagnosis of mild pneumonia, respectively, as shown in table 3. Wherein, cure: the clinical symptoms disappear and the normal state is restored. The effect is shown: the signs of the clinical symptoms are substantially or mostly disappeared. The method is effective: the clinical symptoms are obviously improved. Invalidation: there was no improvement in clinical symptoms.

Table 3 therapeutic effects of each group of drugs

	Example 1	Example 4	Example 7	Example 10
					Cure (example)	8	8	7	8
Display effect (example)	1	1	1	1
					Effective (example)	1	0	1	1
Invalidation (example)	0	1	1	0
					Effective rate of	100％	90％	80％	90％

Clinical experiments show that the medicine has the cure rate of more than 90 percent for mild pneumonia, and has fewer invalid cases and good treatment effect.

The embodiments described above are some, but not all embodiments of the invention. The detailed description of the embodiments of the invention is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

Claims

1. The pharmaceutical composition for treating the H1N1 influenza virus pneumonia is characterized by comprising the following components in parts by weight: 10-15 parts of fritillaria cirrhosa, 2-10 parts of ephedra, 10-20 parts of loquat leaf, 5-10 parts of peppermint, 5-10 parts of weeping forsythiae capsule, 2-5 parts of poria cocos, 2-5 parts of houttuynia cordata, 10-15 parts of honeysuckle, 2-5 parts of baical skullcap root, 1-5 parts of gardenia, 5-15 parts of bupleurum, 2-10 parts of white mulberry root-bark, 5-10 parts of pilose asiabell root, 2-5 parts of oyster, 5-6 parts of chrysanthemum, 4-5 parts of liquorice, 5-6 parts of angelica, 2-5 parts of ligusticum chuanxiong, 2-10 parts of radix sileris, 2-5 parts of horseshoe, 5-10 parts of dried orange peel, 2-5 parts of dendrobium officinale, 5-10 parts of beta-cyclodextrin and 5-10 parts of cellulose.

2. A method of preparing a pharmaceutical composition for the treatment of H1N1 influenza virus pneumonia according to claim 1, comprising the steps of:

s1, soaking the raw materials in a solvent, then decocting for 5-6 hours at 90-110 ℃, and filtering to obtain filtrate and filter residues;

s4, uniformly mixing the mixture obtained in the step S3, the extracting solution, cellulose and the other part of beta-cyclodextrin, drying and granulating;

the solvent consists of cellulase, hemicellulase, pectinase and water;

the mass ratio of the cellulase to the hemicellulase to the pectase is 1:0.5-1:0.2-0.5;

in the step S2, the particle size of the crushed filter residues is 200-300 meshes, and the volume fraction of the ethanol water solution is 50-60%;

the mass ratio of the beta-cyclodextrin in the step S3 to the beta-cyclodextrin in the step S4 is 1:1-2.