CN114916549A - Plant growth regulating composition, preparation and application thereof - Google Patents

Plant growth regulating composition, preparation and application thereof Download PDF

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Publication number
CN114916549A
CN114916549A CN202110874711.1A CN202110874711A CN114916549A CN 114916549 A CN114916549 A CN 114916549A CN 202110874711 A CN202110874711 A CN 202110874711A CN 114916549 A CN114916549 A CN 114916549A
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Prior art keywords
plant growth
regulating composition
growth regulator
active ingredient
growth regulating
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CN114916549B (en
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高祥文
李向阳
赵圣英
张晶晶
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Shandong Shibang Agrochemical Co ltd
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Shandong Shibang Agrochemical Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/22—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
    • A01N43/647—Triazoles; Hydrogenated triazoles
    • A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/24—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
    • A01N47/36—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< directly attached to at least one heterocyclic ring; Thio analogues thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
    • A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Toxicology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to the technical field of pesticides, and particularly relates to a plant growth regulator composition, a preparation and application thereof. The composition comprises thidiazuron and brassinolide; the thidiazuron and brassinolide; the mass ratio of the thidiazuron to the brassinolide is (1.6-135): 1. the preparation prepared from the plant growth regulator composition and the auxiliary materials acceptable in the pesticide pharmacy has a good regulating effect on the yield of crops, and greatly reduces the loss and damage of chemical pesticides to the crops; meanwhile, the composition has no resistance, higher activity, low using amount of weapons and low using cost.

Description

Plant growth regulating composition, preparation and application thereof
Technical Field
The invention relates to the technical field of pesticides, in particular to a plant growth regulating composition, a preparation and application thereof, wherein the IPC classification number is A01N 43/653.
Background
Thidiazuron is a plant photosynthesis promoter, and has an obvious effect of increasing yield. The thidiazuron can promote cell division and fruit expansion. The growth promoter has obvious promotion effect on cell division and transverse growth and longitudinal growth of organs, thereby playing a role in expanding fruits; can delay aging of leaves, keep green for a long time, strengthen chlorophyll synthesis, improve photosynthesis, and promote the color of leaves to deepen and turn green; breaking the apical dominance, promoting lateral bud germination, guiding bud differentiation, promoting lateral branch generation, increasing branch number, increasing flower number, improving pollen fertility, increasing fruit number and increasing yield; improve the quality of crops and improve the marketability. Inducing parthenocarpy, stimulating ovary to expand, preventing flower and fruit drop, promoting protein synthesis, and increasing sugar content. The wheat is sprayed before winter, so that tillering can be promoted, freezing injury can be resisted, and herbicide damage can be relieved; the green-turning period can resist spring coldness, increase secondary tillering and improve the spike number per mu; the spray at the initial stage of flower raising can resist dry hot air and green branch wax stems, and the thousand seed weight is increased. The herbicide can also be used for increasing the yield of corn, peanut, rice, ginger, apple, bean, strawberry, vegetable and the like, has stable effect under different climatic and ecological environment conditions, has the defects of different degrees in the agricultural weeding process of the herbicide variety containing a single active ingredient, but is easy to generate drug resistance after being continuously used for a long time, has a broad weeding spectrum and cannot prevent both diseases.
The invention provides a plant growth regulating composition containing thidiazuron and brassinolide, which not only improves the yield of crops and relieves the defects of low activity and drug resistance of some regulators in the market, but also obviously improves the stability of the plant growth regulator by optimizing auxiliary materials such as an auxiliary agent, an emulsifier and the like.
Disclosure of Invention
In order to solve the above technical problems, the present invention provides, in a first aspect, a plant growth regulating composition comprising 6 wt% to 7 wt% of an active ingredient a, wherein the active ingredient a is brassinolide and a derivative thereof.
As a preferred technical scheme of the invention, the plant growth regulating composition comprises 6 to 7 weight percent of active ingredient A1 with the following structure,
the structure of A1 is:
Figure BDA0003190183000000021
as a preferable technical scheme of the present invention, the plant growth regulating composition further comprises an active ingredient B, wherein the active ingredient B is selected from one of thidiazuron, pyraclostrobin, uniconazole, diethyl aminoethyl hexanoate, gibberellic acid, S-abscisic acid, polyoxin, kasugamycin, indoleacetic acid, forchlorfenuron and ethephon.
As a preferable technical scheme of the invention, the plant growth regulating composition comprises an active ingredient B and an active ingredient A which have a mass ratio of (1.6-135) to 1.
As a preferable technical scheme of the invention, the plant growth regulating composition comprises thidiazuron and an active ingredient A in a mass ratio of (1.6-135):1.
As a preferred technical scheme of the invention, the plant growth regulating composition comprises thidiazuron and an active ingredient A in a mass ratio of 15: 1.
In a second aspect, the present invention provides a plant growth regulator comprising from 0.1 to 0.5 wt% of any one of the plant growth regulating compositions described above.
As a preferable technical scheme of the invention, the formulation of the plant growth regulator is selected from one of wettable powder, dispersible oil suspending agent, soluble solution and microemulsion.
As a preferred technical scheme of the invention, the plant growth regulator also comprises an auxiliary agent with the following structural formula (1):
Figure BDA0003190183000000022
wherein R is 1 H or C1-C5 alkyl, R 2 (ii) C1-C3 alkyl, R 3 C1-C3 alkyl.
In a third aspect, the present invention provides the use of a plant growth regulating composition in the field of agriculture to promote or regulate plant growth.
Has the advantages that:
1. the thidiazuron and the 28-high brassinolide are compounded for use, so that the synergistic effect on crops is obvious, the yield of the crops is well regulated, and the loss and the damage of chemical pesticides to the crops are greatly reduced;
2. the growth regulator soluble solution has obvious promotion and synergy effects on crops under the dosage of 0.32-0.53mg/Kg, simultaneously, the compositions have no resistance and higher activity, and the weapons have low usage amount and low use cost;
3. in the invention, by selecting a specific auxiliary agent and controlling the proportion and the addition amount of the auxiliary agent through a large number of experiments, the growth regulator soluble solution has good heat storage stability and cold storage stability, so that the drug effect of the growth regulator soluble solution can be fully exerted;
4. a safety experiment is carried out on the growth regulator soluble solution according to the evaluation criteria of pesticide-to-crop safety (NY/T1965.1-2010), and the experimental result shows the safety of the growth regulator soluble solution to crops.
Detailed Description
In order to solve the above technical problems, the present invention provides, in a first aspect, a plant growth regulating composition comprising 6 wt% to 7 wt% of an active ingredient a, wherein the active ingredient a is brassinolide and a derivative thereof.
In a preferred embodiment, the plant growth regulating composition comprises 6 to 7% by weight of active ingredient A1 of the structure,
the structure of A1 is:
Figure BDA0003190183000000031
in a preferred embodiment, the plant growth regulating composition further comprises an active ingredient B selected from one of thidiazuron, pyraclostrobin, uniconazole, diethyl aminoethyl hexanoate, gibberellic acid, S-abscisic acid, polyoxin, kasugamycin, indoleacetic acid, forchlorfenuron, ethephon.
In a preferred embodiment, the plant growth regulating composition comprises thidiazuron and active ingredient a in a mass ratio of 15: 1.
In a preferred embodiment, the plant growth regulating composition comprises thidiazuron and active ingredient a in a mass ratio of 15: 1.
In a preferred embodiment, the plant growth regulating composition comprises thidiazuron and the active ingredient A1 in a mass ratio of (1.6-135): 1.
In a preferred embodiment, the plant growth regulating composition comprises thidiazuron and active ingredient A1 in a mass ratio of 15: 1.
The structure of thidiazuron is:
Figure BDA0003190183000000041
thidiazuron, also known as defoliant, has strong activity of promoting plant cell division by promoting accumulation and synthesis of catalytic cytokinin in plant tissues and further promoting growth and regeneration of the tissues.
The active ingredient A1 is 28-high brassinolide (28-HBR), which is an active form of brassinolide, and can directly or indirectly scavenge active oxygen in plant body, thereby maintaining plant physiological metabolism. The 28-high brassinolide can mainly regulate the growth of plants through three physiological actions of promoting the division of the plants, promoting the photosynthesis and improving the stress resistance of the plants.
The thidiazuron and the 28-high brassinolide in the invention are both effective pesticide ingredients which can be used in the field of pesticides. The source of which is not limited, and the purchasing manufacturer of thidiazuron includes, but is not limited to, Jiangsu Bandong; the 28-high brassinolide is purchased from a variety of vendors including, but not limited to, Jiangxi Weidi.
In the invention, the inventor discovers through a large number of experiments that the combination of thidiazuron and 28-high brassinolide in the invention, particularly the combination of thidiazuron and 28-high brassinolide with the mass ratio of 15: 1, generates a synergistic effect, can well play a role in increasing the yield of plants, particularly wheat, some plant growth regulators on the market at present have lower activity, general effects and no effect of increasing the yield, and the combination of thidiazuron and 28-high brassinolide with the mass ratio of 15: 1 in the invention not only improves the stress resistance of crops, but also can improve photosynthesis, regulate the vegetative growth and further increase the yield of the crops.
In a second aspect, the present invention provides a plant growth regulator comprising from 0.1 to 0.5 wt% of any one of the plant growth regulating compositions described above.
In a preferred embodiment, the plant growth regulating composition comprises 0.12 to 0.2 wt% of any of the plant growth regulating compositions described above.
In a more preferred embodiment, the plant growth regulating composition comprises 0.16 wt% of any of the plant growth regulating compositions described above.
In one embodiment, the formulation of the plant growth regulator is selected from one of wettable powder, dispersible oil suspension, soluble solution, and microemulsion.
In a preferred embodiment, the plant growth regulator is in the form of a soluble solution.
In a preferred embodiment, the adjuvant of the following structural formula (1):
Figure BDA0003190183000000051
wherein R is 1 H or C1-C5 alkyl, R 2 (ii) C1-C3 alkyl, R 3 C1-C3 alkyl.
In one embodiment, the content of the adjuvant in the plant growth regulation is 10-20 wt%; preferably 13 to 17 wt%; further preferably 15 wt%.
In a preferred embodiment, R 1 ＝H，R 2 C1 alkyl, R 3 A C1 alkyl group, specifically, the structure of the adjuvant is:
Figure BDA0003190183000000052
the chemical name of the compound is N, N-dimethylformamide.
In order to impart excellent properties to a plant growth regulator soluble solution, in one embodiment, the plant growth regulator further comprises an emulsifier selected from at least one of tween series emulsifiers, fatty alcohol-polyvinyl ether series emulsifiers, condensation product series emulsifiers of alkylphenol and ethylene oxide, nonylphenol polyoxyethylene ether series emulsifiers, nonionic series emulsifiers, and BYK series emulsifiers.
As the Tween series emulsifiers, Tween T-80, Tween T-60, Tween T-40 and the like can be exemplified.
In a preferred embodiment, the emulsifier is a non-ionic series of emulsifiers.
In one embodiment, the mass ratio of the emulsifier to the auxiliary agent is 8 to (10-20); preferably 8 to (14-16); further preferred is 8: 15.
In one embodiment, the nonionic series of emulsifiers have an HLB value of from 12 to 16.
The HLB value refers to the hydrophilic-lipophilic balance value, wherein the lower the HLB value is, the stronger the lipophilicity of the emulsifier is; the higher the HLB value, the more hydrophilic the emulsifier is.
In one embodiment, the non-ionic series of emulsifiers is selected from at least one of the group consisting of 600 emulsifiers, 601 emulsifiers, 602 emulsifiers, 603 emulsifiers.
In a preferred embodiment, the non-ionic series of emulsifiers are 602 emulsifiers.
The stability has great influence on the drug effect of the plant growth regulator soluble solution, particularly the stability of the raw drug, when the raw drug is recovered to be crystallized and the preparation is layered, the phenomenon is usually irreversible, different emulsifiers and auxiliary agents are selected according to different systems, a great amount of experiments show that the mutual synergistic action of 602 emulsifier and N, N-dimethylformamide in the system can well increase the heat storage stability of the plant growth regulator soluble solution, probably because thidiazuron in the system can be well dissolved in water to form a water phase, and 28 high brassinolide can be well dissolved in N, N-dimethylformamide to form an organic phase, and the polyoxyethylene in 602 emulsifier forms a shell structure in the system to further promote the acting force between the N, N-dimethylformamide and 28 high brassinolide, meanwhile, the alkyl chain on the N, N-dimethylformamide is shorter, so that larger steric hindrance cannot be caused, the 602 emulsifier with the HLB value of 12-16 can well reduce the interfacial tension of water, so that a system can be well fused together, and the 602 emulsifier has a proper cloud point, so that the phenomenon of merging and aggregation of liquid drops in a water phase due to the fact that Brownian motion is accelerated at a higher temperature can be prevented.
However, through a lot of experiments, it is found that in the system of the present invention, the more the amount of the 602 emulsifier is, the better the amount is, when the amount of the 602 emulsifier is too much, the stability of the plant growth regulator soluble solution is adversely affected, probably because the emulsifier is more capable of forming an external phase with an aqueous phase to form an O/W stable system with an organic phase, but when the amount of the 602 emulsifier is too much, a state that the density of the external phase is different from that of the organic phase (internal phase) is easy to occur, and at a higher temperature, the movement of some micro-droplets in the system is accelerated, which easily causes the system to be uneven, thereby affecting the stability; similarly, N-dimethylformamide needs to be mixed with an emulsifier in a certain ratio to maintain the stability of the system.
In one embodiment, the plant growth regulator further comprises a C1-C6 alcohol (such as methanol, ethanol, propanol, butanol, pentanol, etc.).
In a preferred embodiment, the weight ratio of the C1-C6 alcohol to the emulsifier is (7-12) to 8; more preferably (9-11) to 8; more preferably 10: 8.
In a preferred embodiment, the plant growth regulator further comprises a C2 alcohol, in particular ethanol.
Through a large number of experiments, the inventor finds that the addition of a certain amount of N, N-dimethylformamide can synergistically act with 602 emulsifier to increase the heat storage stability of the plant growth regulator soluble solution, but the N, N-dimethylformamide is possibly unfavorable for the low-temperature stability of the system because of strong polarity, and the 602 emulsifier is possibly unfavorable for the low-temperature stability of the plant growth regulator soluble solution because of strong hydrophilicity. The inventor unexpectedly finds that short-chain alcohol, especially methanol and ethanol, can synergistically act with 602 emulsifier to reduce interfacial tension, can be dissolved in water to form hydrogen bonds with aqueous solution, and can also form hydrogen bonds with amino molecules in thidiazuron, so that the stability of the system is improved, and the synergistic effect of the short-chain alcohol and N, N-dimethylformamide can well reduce the amount of N, N-dimethylformamide, and can well prolong the time of precipitating and crystallizing effective components of the preparation by low-temperature storage.
However, the applicant found in experiments that the more the amount of ethanol is, the better the amount of ethanol is, when the amount of ethanol is too much, the hot storage stability and the cold storage stability of the plant growth regulator soluble solution can be affected, which may be because the amount of ethanol is too much, the amount of ethanol in the system can be increased gradually, the droplets in the system are in a non-uniform state, the droplets are aggregated to cause the phenomenon of layering, non-uniformity and the like of the plant growth regulator soluble solution, and may be because the boiling point of ethanol is low, and in an environment with higher temperature, ethanol is diffused in the system and then is spatially overlapped with other droplets in the system to cause coalescence.
The remaining raw material in 100 wt% of the plant growth regulator is water.
In one embodiment, the plant growth regulator is prepared by a method comprising: weighing the regulator composition, the auxiliary agent, the emulsifier, the C1-C6 alcohol and water according to the corresponding weight, stirring, mixing, settling and filtering to obtain the plant growth regulator.
In one embodiment, the plant growth regulator soluble agent can be used after being diluted by 500-1500 times of water when in use, and specifically, the dosage of the plant growth regulator soluble agent when in use is 0.2-0.7 mg/Kg; preferably, the dose of the plant growth regulator soluble agent when in use is 0.32-0.53 mg/Kg.
The present plant growth regulator has low activity and relatively poor effect, and after long-term use, plant can produce resistance to it, and through great amount of research, the inventor finds that the soluble agent of the plant growth regulator in the system of the present invention has obvious promoting and yield increasing effect in the dosage of 0.32-0.53 mg/Kg.
In a third aspect, the present invention provides the use of a plant growth regulating composition in the field of agriculture to promote or regulate plant growth.
The present invention will be specifically described below by way of examples. The active ingredients in the invention are all effective pesticide ingredients which can be used in the pesticide field, and the auxiliary agents, the emulsifying agents and the C1-C6 alcohol which are used in the invention are all auxiliary materials which can be accepted in the pesticide field.
In addition, the starting materials used are all commercially available, unless otherwise specified.
Examples
Example 1
A plant growth regulator, the preparation raw materials of the said plant growth regulator, including 0.16 wt% plant growth regulating composition, 15 wt% compounding chemicals, 8 wt% emulsifier, 10 wt% ethanol, water make up to 100 wt%;
the growth regulating composition comprises thidiazuron and 28-high brassinolide, and the mass ratio of the thidiazuron to the 28-high brassinolide is 15: 1;
the auxiliary agent is N, N-dimethylformamide;
the mass ratio of the emulsifier to the auxiliary agent is 8: 15; the emulsifier is a nonionic series emulsifier; the HLB value of the nonionic series emulsifier is 12-16; the non-ionic series of emulsifiers is 602 emulsifiers;
the weight ratio of the ethanol to the emulsifier is 10: 8;
the preparation method of the plant growth regulator comprises the following steps: mixing the plant growth regulating composition, the auxiliary agent, the emulsifier, the ethanol and the water under stirring, settling and filtering to obtain the plant growth regulator.
The purchase manufacturer of thidiazuron is Jiangsu Lvbang; the 28-high brassinolide purchasing manufacturer is Jiangxi Weidi.
Example 2
A plant growth regulator according to the embodiment of example 1, except that 600 emulsifier was used instead of 602 emulsifier.
Example 3
A plant growth regulator was prepared in the same manner as in example 1, except that BYK-120 emulsifier was used instead of 602 emulsifier.
Example 4
A plant growth regulator is prepared from 0.16 wt% of plant growth regulating composition, 15 wt% of auxiliary agent, 15 wt% of emulsifier, 10 wt% of ethanol, and water, wherein the weight of the mixture is 100 wt%, and the rest of the embodiments are the same as those in example 1.
Example 5
A plant growth regulator according to the embodiment same as that of example 1, except that toluene was used instead of N, N-dimethylformamide.
Example 6
A plant growth regulator, the preparation raw materials of the said plant growth regulator, including 0.16 wt% plant growth regulating composition, 18 wt% compounding chemicals, 8 wt% emulsifier, 7 wt% ethanol, water make up to 100 wt%; the mass ratio of the emulsifier to the auxiliary agent is 8: 18; the weight ratio of ethanol to emulsifier is 7: 8, and the rest of the embodiment is the same as that of example 1.
Example 7
A plant growth regulator, the preparation raw materials of the said plant growth regulator, including 0.16 wt% plant growth regulating composition, 10 wt% compounding chemicals, 8 wt% emulsifier, 15 wt% ethanol, water make up to 100 wt%; the mass ratio of the emulsifier to the auxiliary agent is 8: 10; the weight ratio of ethanol to emulsifier is 15: 8, and the other implementation modes are the same as example 1.
Performance testing
1. Field efficacy test for regulating wheat growth
1.1 test basis: agricultural industry standards of the people's republic of China "quality management standards for pesticide registration field efficacy test" (NY/T2885-2016), "criteria for pesticide field efficacy test" (GB/T17980.132-2004), and "quality management standards for pesticide registration test" (GB/T17980.132-2004).
1.2 test and control agents: the plant growth regulator of example 1 is soluble; 0.1 wt% thidiazuron soluble agent (from Sichuan Guanghua agrichemical Co., Ltd.), 0.0075 wt% brassinolide aqueous agent (from Jinan Shibang agrichemical Co., Ltd.); and setting clear water contrast.
1.3 selection of crops and cultivars: the crop is winter wheat, Jimai 22; the crop cultivation mode is direct seeding.
1.4 soil type of test field: the test plot has the advantages of flat topography, good planting conditions, uniform fertility, loam as soil, 1% of soil organic matter content and 7.0 of soil pH value.
1.5 test design of the test agents for the test agents is shown in Table 1, wherein the blank control treatment group was identical to the other treatment groups in the methods of spraying equal amount of clear water, applying chemicals, investigating and field managing.
TABLE 1
Figure BDA0003190183000000101
1.6 cell area and repetition: the area of the cell is 20 square meters, and the cell is repeated for 4 times.
1.7 application method: the stem and leaf are sprayed with the pesticide, and the amount of the sprayed pesticide is 450 liters per hectare.
1.8 application time and times: the medicine is applied 1 time in the afternoon of 29 days 4 months in 2019, and the wheat is in the booting stage when the medicine is applied; the medicine is applied 1 time in the afternoon of 5 and 22 months in 2019, and the wheat is in the irrigation period during the application.
1.9 investigation time and number of surveys: 3d, 7d and 10d after the application of the pesticide, visually observing the wheat phytotoxicity symptom; measuring yield and quality at 6 months and 14 days of crop harvest; investigating the effective spike number and the number of grains per spike at the harvest time of 14 days in 6 months; thousand kernel weight was measured after harvest.
1.10 methods of investigation: according to GB/T17980.132-2004, 5 points are taken in each cell during wheat harvesting, each point is 1 square meter, the effective spike number, the solid grain number of each spike and the thousand grain weight are investigated, and an average value of 5 points is obtained; when wheat is harvested, 5 points are taken in each cell, each point is 2 square meters, and the seeds are tested for yield; the quality measurement was carried out by dividing the treatment into 5 ears at random per cell for each treatment, and the quality measurement was carried out after mixing.
1.11 method for calculating drug effect: according to the standard GB/T17980.132-2004; yield increase calculation formula: yield increase (%) (treatment zone yield-clear water control yield)/clear water control yield × 100; the log data were analyzed for random block variance using SPSS19.0, and the multiple comparison method was the dunken method.
1.12 effects on non-target organisms: visual inspection had no effect on other non-target organisms.
1.13 taking 5 points per cell when harvesting wheat, wherein each point is 2 square meters, and testing seeds and yield; the wheat in the sampling points is harvested respectively, threshed in a laboratory, and weighed respectively after the moisture content is lower than 13 percent, so as to obtain the yield of winter wheat of 10 square meters in each cell, and further convert the yield (kg/ha).
1.14 Experimental results and analysis
As shown in table 2, pharmacodynamic analysis: after the wheat is treated by 0.32 mg/kg, 0.4 mg/kg, 0.53mg/kg and 0.8 mg/kg of the active ingredients of the plant growth regulator soluble agent in the embodiment 1, the number of the effective spikes of each medicament treatment and the clear water control group are obviously higher than that of the clear water control group on the level of P0.05; the number of the grains per ear of each treatment and the clear water control group are obviously higher than those of the clear water control group on the level P of 0.05, the number of the grains per ear of each treatment has no obvious difference on the level P of 0.05, and the thousand grain weight of each treatment is higher than that of the clear water control group.
As shown in table 4, the quality analysis of each treatment showed no significant difference in the protein, soluble sugar, starch, and fat content between treatments from the clean water control group at the P-0.05 level.
As shown in tables 2 to 4, the plant growth regulator in example 1, i.e., the soluble agent and 0.0075 wt% brassinolide aqua star coin, had substantially the same effects on yield, quality and the like.
And (3) analyzing a yield measurement result: the yield of each treatment of the plant growth regulator solubles of example 1 was significantly higher than that of the clear water control, with the highest yield for the 0.53mg/kg and 0.8 mg/kg treatments, and the yields of the four treatments were 5.06%, 5.08%, 8.65%, 8.57%, respectively.
TABLE 2 Effect of plant growth regulator solubles in example 1 on wheat yield structure
Processing number Effective spike number (number) Number of solid grains per ear Thousand Ke weight (g)
1 681.25a 29.55ab 40.31bc
2 689.75a 30.45a 40.40b
3 697.05a 29.60ab 41.20a
4 697.15a 29.60ab 40.47b
5 685.45a 29.20b 39.77cd
6 657.25a 30.25a 39.53d
7 625.95b 28.25c 39.57d
TABLE 3 Effect of the plant growth regulator solubles of example 1 on wheat texture
Processing number Yield (kg/ha) Yield increase (%)
1 7472.5Bc 5.06
2 7545.0Bb 6.08
3 7727.5Aa 8.65
4 7722.5Aa 8.57
5 7487.5Bbc 5.27
6 7495.0Bbc 5.38
7 7112.5Cd -
TABLE 4 Effect of plant growth regulator solubles in example 1 on wheat quality
Processing number Protein (%) Soluble sugar (%) Starch (%) Fat (%)
1 10.65Aa 5.44Aa 64.46Aa 1.64Aa
2 10.66Aa 5.45Aa 64.50Aa 1.62Aa
3 10.64Aa 5.42Aa 64.52Aa 1.65Aa
4 10.67Aa 5.43Aa 64.47Aa 1.63Aa
5 10.69Aa 5.40Aa 64.48Aa 1.67Aa
6 10.67Aa 5.44Aa 64.44Aa 1.65Aa
7 10.65Aa 5.41Aa 64.40Ab 1.65Aa
2. Stability in Heat storage
The plant growth regulator-soluble agent in some examples was left at 54 ℃ for 14 days and then removed to observe whether or not delamination and precipitation occurred.
3. Stability in Cold storage
The plant growth regulator soluble solution in some examples was left at-10 ℃ for 14 days and then taken out, and after returning to room temperature, it was observed whether or not delamination and precipitation occurred.
The test results are shown in table 5:
TABLE 5
Stability in Heat storage Stability in cold storage
Example 1 No delamination and no precipitation No delamination and no precipitation
Example 2 Demixing without precipitation /
Example 3 More layers and deposits /
Example 4 Demixing and a little precipitation /
Example 5 Demixing and a little precipitation /
Example 6 / No demixing and little precipitation
Example 7 / Demixing and a little precipitation
The foregoing examples are merely illustrative and serve to explain some of the features of the method of the present invention. The appended claims are intended to claim as broad a scope as is contemplated, and the examples presented herein are merely illustrative of selected implementations in accordance with all possible combinations of examples. Accordingly, it is applicants' intention that the appended claims are not to be limited by the choice of examples illustrating features of the invention. Also, where numerical ranges are used in the claims, subranges therein are included, and variations in these ranges are also to be construed as possible being covered by the appended claims.

Claims (10)

1. A plant growth regulating composition is characterized by comprising 6-7 wt% of an active ingredient A, wherein the active ingredient A is brassinolide and a derivative thereof.
2. The plant growth regulating composition according to claim 1, characterized in that it comprises 6 to 7% by weight of active ingredient A1 of the structure,
the structure of A1 is:
Figure FDA0003190182990000011
3. the plant growth regulating composition according to claim 1 or 2, further comprising an active ingredient B selected from one of thidiazuron, pyraclostrobin, uniconazole, diethyl aminoethyl hexanoate, gibberellic acid, S-abscisic acid, polyoxin, kasugamycin, indoleacetic acid, forchlorfenuron, ethephon.
4. Plant growth regulating composition according to claim 1 or 2, characterized in that it comprises, in mass ratio (1.6-135): 1 and active ingredient B and active ingredient a.
5. Plant growth regulating composition according to claim 1 or 2, characterized in that it comprises, in mass ratio (1.6-135): 1 and an active ingredient a.
6. Plant growth regulating composition according to claim 1 or 2, characterized in that it comprises, in a mass ratio of 15: 1 and an active ingredient a.
7. A plant growth regulator, characterized in that it comprises 0.1-0.5 wt% of the plant growth regulating composition according to any one of claims 1 to 5.
8. The plant growth regulator according to claim 7, wherein the formulation of the plant growth regulator is selected from one of wettable powder, dispersible oil suspension, soluble solution and microemulsion.
9. The plant growth regulator according to claim 7, further comprising an adjuvant of the following structural formula (1):
Figure FDA0003190182990000012
wherein R is 1 H or C1-C5 alkyl, R 2 (ii) C1-C3 alkyl, R 3 C1-C3 alkyl.
10. Use of a plant growth regulating composition according to any one of claims 1 to 5 in the agricultural field to promote or regulate plant growth.
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