CN112806373A

CN112806373A - Pesticide composition for promoting crop yield increase and preparation method thereof

Info

Publication number: CN112806373A
Application number: CN202011598520.9A
Authority: CN
Inventors: 吴通; 张海旺; 况候义; 熊伟
Original assignee: Jiangxi Huawei Technology Co ltd
Current assignee: Jiangxi Huawei Technology Co ltd
Priority date: 2020-12-30
Filing date: 2020-12-30
Publication date: 2021-05-18

Abstract

The invention discloses a pesticide composition for promoting crop yield increase and a preparation method thereof. Belongs to the technical field of pesticides. The pesticide composition comprises the following components in parts by weight: 1-3000 parts of 28-homobrassinolide and 1-3000 parts of gibberellic acid. Compared with the prior art, the invention has the following beneficial effects: the 28-high brassinolide and the gibberellic acid are compounded for use, so that the synergistic effect is achieved within a certain proportion range, and the yield increase of crops can be promoted.

Description

Pesticide composition for promoting crop yield increase and preparation method thereof

Technical Field

The invention relates to the technical field of pesticides, in particular to a pesticide composition for promoting crop yield increase and a preparation method thereof.

Background

China is a big agricultural country and has a plurality of varieties of planted crops. The agricultural production efficiency is improved, and the method is a good matter benefiting the nation and the people in the aspect of agriculture.

Plant growth regulators are widely applied to vegetable production in recent years, and the use of plant regulators is common. However, the regulation effect of a single pesticide on crops is not obvious, and the compounding of different pesticide varieties is a common method in modern agriculture.

In conclusion, the problem to be solved by the technical personnel in the field is how to provide a pesticide composition for promoting the yield increase of crops and a preparation method thereof.

Disclosure of Invention

In view of the above, the invention provides a pesticide composition for promoting crop yield increase and a preparation method thereof. The 28-high brassinolide and the gibberellic acid are compounded, so that the 28-high brassinolide and the gibberellic acid have a synergistic effect, the growth of crops can be regulated, and the yield of the crops is increased.

In order to achieve the purpose, the invention adopts the following technical scheme:

a pesticide composition for promoting crop yield increase comprises the following components in parts by weight: 1-3000 parts of 28-homobrassinolide and 1-3000 parts of gibberellic acid.

28-high brassinolide is one of steroid compounds with higher biological activity, and is widely present in plants. Can promote cell division and elongation, improve enzyme activity, improve seed activity, promote early development, regulate crop growth, and relieve injury during freezing injury, drought, and phytotoxicity. During each stage of plant growth and development, it can promote the vegetative growth and is favorable for fertilization. The artificially synthesized brassinolide has high activity, can be absorbed by leaves, stems and roots of plants and then is conducted to acting parts, and the acting concentration is trace.

Gibberellic acid is a broad-spectrum plant growth regulator, and can promote the growth and development of crops, make the crops mature in advance, improve the yield and improve the quality; can rapidly break the dormancy of organs such as seeds, tubers and bulbs and promote germination; reduce the falling of buds, flowers, bolls and fruits, improve the fruit bearing rate or form seedless fruits. Some 2-year-old plants can also flower in the same year.

The 28-high brassinolide and the gibberellic acid are compounded, and the 28-high brassinolide and the gibberellic acid are synergistic.

Preferably, the pesticide composition for promoting the yield increase of crops comprises the following components in parts by weight: 1-1600 parts of 28-high brassinolide and 1-1600 parts of gibberellic acid.

Preferably, the pesticide composition for promoting the yield increase of crops comprises the following components in parts by weight: 28-homobrassinolide 1 part and gibberellic acid 165 parts.

Preferably, the mass concentration of the 28-high brassinolide is 0.015mg/L, and the mass concentration of the gibberellic acid is 2.475 mg/L.

Preferably, the gibberellic acid is one or more of gibberellic acid A3, gibberellic acid A4, gibberellic acid 7, and gibberellic acid A4+ 7.

Preferably, the pesticide composition can be any one of a suspending agent, a water dispersible granule, an aqueous emulsion, a microemulsion, a wettable powder, a granule and a soluble solution.

Preferably, the composition further comprises auxiliary components.

Preferably, the auxiliary ingredients are one or more of solvents, emulsifiers, wetting agents, stabilizers, disintegrants, dispersants, thickeners, pH regulators, defoamers, antifreeze and filler agents.

The solvent is one or more of methyl oleate, fatty acid methyl ester, pine-based oil, soybean oil, biodiesel, sec-butyl acetate, dimethyl carbonate, diesel oil, white oil, engine oil, absolute ethyl alcohol, glycerol, N-butanol, dimethyl sulfoxide, acetone, cyclohexanone and N-methylpyrrolidone.

The emulsifier is one or more of calcium dodecyl benzene sulfonate, fatty alcohol polyoxyethylene ether, triphenylethylphenol polyoxypropylene polyoxyethylene block polymer, dibenzyl biphenyl polyoxyethylene ether, phenethyl phenol polyoxyethylene ether, castor oil polyoxyethylene ether, phenethyl phenol formaldehyde resin polyoxyethylene ether, phenethyl phenol polyoxypropylene polyoxyethylene ether, alkylaryl polyoxypropylene polyoxyethylene ether, phenethyl phenol formaldehyde resin polyoxyethylene ether, sorbitan fatty acid ester polyoxyethylene ether, natural fatty alcohol and ethylene oxide adduct, fatty alcohol and ethylene oxide condensate, polyoxyethylene fatty acid ester and alkyl polyglycoside.

The wetting agent is one or more of sodium butyl naphthalene sulfonate, sodium dodecyl sulfate, fatty alcohol polyoxyethylene (6-7) ether, dioctyl sodium sulfosuccinate, Morwet EFW and alkyl naphthalene sulfonate.

The disintegrating agent is one or more of sodium bicarbonate, magnesium chloride, aluminum chloride, sodium chloride, urea, ammonium sulfate, and bentonite.

The dispersant is one or more of formaldehyde condensate of sodium methyl naphthalene sulfonate, sodium lignosulfonate, calcium lignosulfonate, p-methoxyl fatty amido benzenesulfonic acid, desugarized and graded sodium lignosulfonate, sodium salt of naphthalene sulfonic acid condensate, sodium salt of alkyl naphthalene sulfonic acid condensate and alkylphenol polyoxyethylene ether formaldehyde condensate sulfuric acid.

The thickener is one or more of xanthan gum, magnesium aluminum silicate, bentonite and white carbon black.

The pH regulator is one or more of ammonium hydroxide, potassium hydroxide, sodium hydroxide, hydrochloric acid, acetic acid, citric acid, and phosphoric acid.

The stabilizer is one or more of epoxidized soybean oil, epichlorohydrin, butyl hydroxy anisole, dibutyl hydroxy toluene, propyl gallate and tert-butyl hydroquinone.

The defoaming agent is one or more of silicone compound, methanol, silicone oil and kasong.

The antifreezing agent is one or more of glycerol, ethylene glycol, propylene glycol, sorbitol, and polyethylene glycol.

The carrier is one or more of kaolin, diatomite, bentonite, white carbon black, light calcium carbonate, attapulgite, clay, talcum powder, quartz sand, starch, soluble starch and potassium fulvate.

The binder is one or more of a naphthalene sulfonate formaldehyde condensate aqueous solution, a naphthalene sulfonic acid condensate sodium salt, an alkyl naphthalene sulfonic acid condensate sodium salt and water.

A preparation method of a pesticide composition for promoting crop yield increase can select any one of the following preparation steps:

firstly, mixing auxiliary components, uniformly shearing, then adding 28-high brassinolide and gibberellic acid, and grinding for 2-3 hours to enable the particle diameters to be below 5mm, so as to obtain a pesticide composition suspending agent preparation;

mixing 28-high brassinolide, gibberellic acid, solvent and emulsifier together, and dissolving to form uniform oil phase; then mixing water, a stabilizing agent, an antifreezing agent, a thickening agent and a pH regulator together, and dissolving the mixture into a uniform water phase; then adding the oil phase into the water phase while stirring at a high speed of 1800 rpm, slowly adding water until reaching a phase inversion point, then performing high-speed shearing at 2900 rpm, adding the rest water, and shearing for 30min to obtain the pesticide composition aqueous emulsion preparation;

dissolving 28-high brassinolide and gibberellic acid completely with solvent and cosolvent, adding emulsifier, stabilizer, antifreezing agent, thickener and pH regulator, mixing, adding water, and stirring to obtain microemulsion;

mixing all the raw materials, fully stirring, crushing by a jet mill, and uniformly mixing to obtain the wettable powder preparation of the pesticide composition;

uniformly mixing all the raw materials, crushing, kneading, granulating, drying and screening to obtain the pesticide composition water dispersible granule preparation;

sixthly, mixing the raw materials, adding water, kneading, molding, drying and screening to obtain the pesticide composition granule preparation;

seventhly, dissolving 28-high brassinolide and gibberellic acid completely by using a solvent and a cosolvent, then adding an emulsifier, a stabilizer, an antifreezing agent, a thickening agent and a pH regulator, uniformly mixing, finally adding water, and fully stirring to obtain the pesticide composition solution preparation.

According to the technical scheme, compared with the prior art, the invention has the following beneficial effects: the 28-high brassinolide and the gibberellic acid are compounded for use, so that the synergistic effect is achieved within a certain proportion range, and the yield increase of crops can be promoted.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.

FIG. 1 is a graph showing the results of the field efficacy test report in example 9 of the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The required medicament is a conventional experimental medicament purchased from a market channel; the unrecited experimental method is a conventional experimental method, and is not described in detail herein.

Example 1

The pesticide composition is processed into a suspending agent, and the mass percentage of each component is shown in table 1.

TABLE 1 suspending agent formulation

The specific operation steps are as follows: the auxiliary components are mixed, sheared and mixed evenly in a shearing machine, then 28-high brassinolide and gibberellic acid are added, and the mixture is ground in a ball mill for 2.5 hours to ensure that the particle diameter is below 5mm, thus preparing the pesticide composition suspending agent preparation.

Example 2

The pesticide composition is processed into an aqueous emulsion, and the mass percentage of each component is shown in table 2.

TABLE 2 aqueous emulsion formulations

The specific operation steps are as follows: firstly, 28-homobrassinolide, gibberellic acid, a solvent and an emulsifier are mixed and dissolved into a uniform oil phase. Then mixing part of water and other auxiliary components together, and dissolving them to obtain uniform water phase. And then adding the oil phase into the water phase while stirring at a high speed (1800 revolutions per minute) in a reaction kettle, slowly adding water until reaching a phase inversion point, then carrying out high-speed shearing (2900 revolutions per minute), adding the rest water, and shearing for 30min to obtain the pesticide composition aqueous emulsion preparation.

Example 3

The pesticide composition is processed into microemulsion, and the mass percentage of each component is shown in table 3.

TABLE 3 microemulsion formulation

Components	Mass percentage of
		28-homobrassinolide + gibberellic acid	5(0.03+4.97)
Dimethyl sulfoxide	8
		Alkylaryl polyoxypropylene polyoxyethylene ether	12
Epoxidized soybean oil	4
		Ethylene glycol	5
Xanthan gum	0.1
		Hydrochloric acid	0.2
Water (W)	Balance of

The specific operation steps are as follows: dissolving 28-homobrassinolide and gibberellic acid with solvent completely, adding other auxiliary components, mixing, adding water, stirring, and shearing for 30min to obtain microemulsion preparation.

Example 4

The pesticide composition is processed into wettable powder, and the mass percentage of each component is shown in table 4.

TABLE 4 wettable powder formulation

Components	Mass percentage of
		28-homobrassinolide + gibberellic acid	50(0.3+49.7)
Desugared and fractionated sodium lignosulphonate	9
		Sodium dodecyl sulfate	3
Kaolin clay	Balance of

The specific operation steps are as follows: the raw materials are mixed, fully stirred in a stirring kettle, crushed by a jet mill and uniformly mixed to prepare the wettable powder preparation of the pesticide composition.

Example 5

The pesticide composition is processed into water dispersible granules, and the mass percentage of each component is shown in table 5.

TABLE 5 Water dispersible granule formulation

Components	Mass percentage of
		28-homobrassinolide + gibberellic acid	3.0(0.002+2.998)
Alkyl naphthalene sulfonic acid polycondensate sodium salt	8
		Morwet EFW	5
Ammonium sulfate	23
		Aqueous naphthalene sulfonate formaldehyde condensate solutions	12
Kathon	0.3
		Dibutylhydroxytoluene	0.5
Potassium fulvate	Balance of

The specific operation steps are as follows: the raw materials are uniformly mixed, crushed by an ultramicro jet mill, kneaded, added into a fluidized bed granulation dryer for granulation, drying and screening, and then the pesticide composition water dispersible granule preparation can be prepared.

Example 6

The pesticide composition is processed into granules, and the mass percentage of each component is shown in table 6.

TABLE 6 granule formulation

Components	Mass percentage of
		28-homobrassinolide + gibberellic acid	0.5(0.003+0.497)
Naphthalene sulfonic acid condensate sodium salt	8
		Anhydrous ethanol	5
Acetic acid	3
		Attapulgite	Balance of

The specific operation steps are as follows: uniformly mixing 28-homobrassinolide, gibberellic acid, sodium salt of naphthalene sulfonic acid condensate, absolute ethyl alcohol and acetic acid, spraying the mixture into attapulgite under the condition of stirring, and airing to obtain the pesticide composition granule preparation.

Example 7

The pesticide composition is processed into a soluble solution, and the mass percentage of each component is shown in table 7.

TABLE 7 soluble agent formulation

Components	Mass percentage of
		28-homobrassinolide + gibberellic acid	0.5(0.003+0.497)
Anhydrous ethanol	20
		Alkyl glycosides	8
Propyl gallate	0.8
		Ethylene glycol	8
Xanthan gum	0.1
		Acetic acid	3
Water (W)	Balance of

The specific operation steps are as follows: dissolving 28-homobrassinolide and gibberellic acid with solvent completely, adding other auxiliary components, mixing, adding water, and stirring to obtain solution preparation of pesticide composition.

Example 8

(1) The test agent used 95% 28-homobrassinolide and 90% gibberellic acid A4+ A7.

(2) Cucumber, jin you 35, selecting cucumber seedlings with regular stems and leaves 4-5 leaves for test.

(3) Grouping tests:

firstly, 28-high brassinolide is independently used, and the concentration is 0.01, 0.02 and 0.03mg/L respectively;

② the gibberellic acid is used independently, the concentration is 3, 4.97, 7mg/L respectively

③ 28-high brassinolide and gibberellic acid are mixed for use, the concentration of the mixture is 0.03mg/L and 4.97mg/L respectively;

and fourthly, taking a Tween 80 aqueous solution with the mass concentration of 0.1 percent as a contrast.

(4) Measurement indexes are as follows: fruit setting efficiency and single fruit weight increase rate.

(5) The determination method comprises the following steps: reference is made to the laboratory bioassay experimental guidelines NY/T2061.2-2011 and NY/T2061.5-2016 for pesticides. The experimental agent was treated for 4 replicates per concentration, each replicate being 5 pots.

(6) Data processing: with reference to NY1155.7-2006, Gowing was used to calculate the theoretical fruit set efficiency/theoretical single fruit weight gain rate E0(E0 ═ X + Y-X Y/100) for each treatment mix combination, and then the combined effect of the two mixes on cucumber growth regulation was evaluated in comparison to the measured fruit set efficiency/measured single fruit weight gain rate (E).

When the E-E0 value is more than 10 percent, the synergistic effect is obtained, when the E-E0 value is less than-10 percent, the antagonistic effect is obtained, and when the E-E0 value is between-10 percent and 10 percent, the additive effect is obtained.

(7) The experimental results are as follows: the results of the experiment are shown in Table 8.

TABLE 8 results of the experiment

After the two are mixed, the combined action type of the two is evaluated by a Gowing method, and the treatment that the 28-high brassinolide and gibberellic acid A4+7 is 0.03mg/L +4.97mg/L in the aspects of fruit setting effect and single fruit weight increasing rate is that the E-E0 value is more than 10 percent, the effect is synergistic, the yield synergistic effect is obvious, and the two have certain synergistic effect when the ratio of the two is 1: 165.

Experimental results show that when the ratio of 28-high brassinolide to gibberellic acid is 1: 165, the fruit setting rate of cucumber can be improved, the fruit weight of a single plant can be increased, and the adjusting effect is obvious.

The observation of the experimental process shows that the two medicaments and the treatment of each dosage are safe to the growth of the tested cucumber and have no phytotoxicity.

Example 9 test report of field drug efficacy

(1) And (3) test crops: cucumber/jin you 409

(2) Test start time: 6 months and 11 days 2019; test completion time: year 2019, month 10 and day 16.

(3) The test agents are shown in table 9:

TABLE 9 test Agents

The product of the invention is 0.5% 28-homobrassinogibberellic acid soluble solution, and the product of the invention is 0.4% 28-epihomobrassinogibberellic acid soluble solution, which is a commercially available product for comparison, and is a method for verifying new medicaments in the agricultural sector. The concentrations of the active ingredient contents of the following table were used.

(4) The application method and the water consumption are as follows: spraying, 675 liters of water is used for hectare.

(5) And (3) test results: the test results are shown in table 10.

TABLE 10 results of the field test

Note: the difference grade, Aa is obviously different, Bb is generally different, and Cd is not obviously different

And (4) conclusion: 0.5 percent 28-homobrasyl gibberellic acid GA4+7 soluble solution can regulate the growth of the cucumber, and the cucumber can be recommended to be diluted 2500 times (the effective component is 2.50-3.30mg/kg) and added with 30-50 liters of water per mu for applying the cucumber in the seedling stage, the bud stage and the young fruit stage, wherein the application is carried out 3 times at intervals of 8-18 days. The cucumber pesticide is safe to cucumbers and has no adverse effect on surrounding target organisms and other plant diseases and insect pests.

Example 10 field efficacy test report

(1) And (3) test crops: citrus fruit

(2) Test start time: year 2018, month 5, day 11; test completion time: 11 and 30 months in 2018.

(3) The test agents are shown in table 11:

TABLE 11 reagent

The 3% 28-homocanola gibberellic acid water dispersible granule is the product of the invention, and the 40% gibberellic acid soluble granule is a commercial product for comparison, which is a method for verifying a new medicament by an agricultural department. The concentrations of the active ingredient contents of the following table were used.

(4) The application method and the water consumption are as follows: the stem and leaf are sprayed, and the water consumption is 1.5L/plant.

(5) And (3) test results: the test results are shown in table 12.

Table 12 results of field test

And (4) conclusion: the 3% 28-homobrassinogibberellic acid water dispersible granule regulates the growth of citrus, is suitable for once application in bud period, young fruit period and fruit expansion period, the dosage is 1333-1000 times (the effective component is 22.51-30mg/kg), and the water consumption for stem and leaf spraying is 1.5L/strain. The pesticide is safe to oranges, and has no adverse effect on surrounding target organisms and other plant diseases and insect pests.

The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. The pesticide composition for promoting the yield increase of crops is characterized by comprising the following components in parts by mass: 1-3000 parts of 28-homobrassinolide and 1-3000 parts of gibberellic acid.

2. The agricultural chemical composition for promoting the yield increase of crops as claimed in claim 1, which comprises the following components in parts by mass: 1-1600 parts of 28-high brassinolide and 1-1600 parts of gibberellic acid.

3. The agricultural chemical composition for promoting the yield increase of crops as claimed in claim 1, which comprises the following components in parts by mass: 28-homobrassinolide 1 part and gibberellic acid 165 parts.

4. The agricultural chemical composition for promoting crop yield increase according to claim 3, wherein the mass concentration of 28-high brassinolide is 0.015mg/L, and the mass concentration of gibberellic acid is 2.475 mg/L.

5. The agricultural chemical composition for increasing the yield of crops as claimed in any one of claims 1 to 4, wherein the gibberellic acid is one or more of gibberellic acid A3, gibberellic acid A4, gibberellic acid 7 and gibberellic acid A4+ 7.

6. The agricultural chemical composition for promoting crop yield increase according to any one of claims 1 to 4, wherein the agricultural chemical composition can be any one of a suspending agent, a water dispersible granule, an aqueous emulsion, a microemulsion, a wettable powder, a granule and a soluble agent.

7. The agricultural chemical composition for promoting crop yield increase of claim 6, further comprising an auxiliary component.

8. The agricultural chemical composition for promoting crop yield increase according to claim 7, wherein the auxiliary component is one or more of a solvent, an emulsifier, a wetting agent, a stabilizer, a disintegrant, a dispersant, a thickener, a pH regulator, an antifoaming agent, an antifreezing agent and a filler.

9. The method for preparing a pesticide composition for promoting yield increase of crops as claimed in claim 8, wherein any one of the following preparation steps can be selected: