CN114903042B - Regulator for promoting plant growth at low temperature, preparation method and application - Google Patents
Regulator for promoting plant growth at low temperature, preparation method and application Download PDFInfo
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- CN114903042B CN114903042B CN202210511792.3A CN202210511792A CN114903042B CN 114903042 B CN114903042 B CN 114903042B CN 202210511792 A CN202210511792 A CN 202210511792A CN 114903042 B CN114903042 B CN 114903042B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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Abstract
The application discloses a regulator for promoting plant growth at low temperature, a preparation method and application thereof, wherein the regulator comprises sulfhydryl compound 2, 3-dimercaptosuccinic acid, surfactant and glutamine, 2, 3-dimercaptosuccinic acid (or 2, 3-dimercaptosuccinic acid and glutamine) is dissolved in water, surfactant Triton X-100 is added, and pH is regulated to be neutral to obtain a mixed solution. The regulator for promoting the growth of the plants at low temperature can be sprayed on the front and back surfaces of the leaf surfaces of the plants, so that the tolerance of the plants to low-temperature cold injury can be effectively improved, the plants can have stronger growth vigor and photosynthetic capacity at lower temperature, and the regulator has good application prospect in plant production.
Description
Technical Field
The application relates to a regulator for promoting plant growth at low temperature and application thereof, belonging to the technical field of planting.
Background
Cryo-cold damage is one of the important factors affecting the normal growth and development of plants, leading to their growth and yield decline. The loss of agroforestry crops due to low temperature injury is up to several billion yuan annually. The harm of low temperature to crops is mainly expressed in aspects of cell membrane permeability, osmotic regulation, generation and elimination of active oxygen free radicals and the like.
In view of the serious damage caused by low temperature, especially by the frost of the spring, to agricultural production, how to improve the low temperature resistance of plants and to enhance the frost resistance of the plants has become the focus of attention of researchers. Early researches have been conducted to prevent crops from being damaged by low temperature stress by improving external environmental conditions such as plastic film coverage, material coverage of rice straw and tree branches and leaves, and measures such as smoke formation. How to stimulate the low temperature resistance of plants per se in view of the damage caused by low temperature to plant growth and how to lighten the low temperature frost of crops is an urgent problem to be solved in agricultural production. The application of exogenous substances to plants to increase the cold resistance of the plants is of great importance for increasing crop production.
Disclosure of Invention
The application aims to: in order to solve the problems of the prior art, a first object of the present application is to provide a regulator for promoting plant growth at low temperature, a second object of the present application is to provide a preparation method of the regulator for promoting plant growth at low temperature, and a third object of the present application is to provide an application of the regulator for promoting plant growth at low temperature in low temperature cold resistance of tobacco.
The application is realized by adopting the following technical scheme.
A regulator for promoting plant growth at low temperature, which is disclosed by the application, comprises 2, 3-dimercaptosuccinic acid with the concentration of 6-10 mM.
Further, the regulator for promoting the low-temperature growth of plants comprises 2, 3-dimercaptosuccinic acid and a surfactant, wherein the mass ratio of the 2, 3-dimercaptosuccinic acid to the surfactant Triton X-100 is about 109-182:300.
Further, the regulator for promoting the low-temperature growth of the plants further comprises glutamine, and the mass ratio of the 2, 3-dimercaptosuccinic acid to the glutamine is 1090-1820:219.
Further, the concentration of the 2, 3-dimercaptosuccinic acid is 6-10 mM.
The preparation method of the regulator comprises the following steps: 2, 3-dimercaptosuccinic acid is weighed and dissolved in water, then a surfactant Triton X-100 is added to adjust the pH value to be neutral, and the mixture is obtained in a volumetric flask to be constant volume.
The preparation method of the regulator comprises the following steps: the method comprises the following steps: 2, 3-dimercaptosuccinic acid and glutamine are respectively weighed and dissolved in water, then a surfactant Triton X-100 is added, the pH value is regulated to be neutral, and the mixture is obtained in a volumetric flask for constant volume.
In the preparation method of the regulator, the concentration of the 2, 3-dimercaptosuccinic acid is 6-10 mM.
The technical scheme of the application comprises the application of the regulator in low-temperature cold resistance of tobacco.
The application of the application comprises that the regulator is uniformly sprayed on the front and back surfaces of tobacco leaves and then placed under the low-temperature condition.
The low temperature condition of the application is-2 ℃ -0 ℃ (containing any value in the interval of-1 ℃, -2 ℃ and the like), and the treatment is carried out for 6 hours.
The cold resistance mechanism of the application: after the regulator for promoting plant growth at low temperature is sprayed, the cell membrane permeability of the leaves is obviously reduced at low temperature, so that the damage of low temperature to the cell membrane can be reduced, and the photosynthesis and biomass of the leaves of tobacco plants are promoted to increase, thereby promoting plant growth.
The beneficial effects are that: compared with the prior art, the application has the following remarkable advantages:
(1) The application provides a novel regulator for promoting plant growth at low temperature for the first time. Plant leaf has enhanced cold resistance after spraying with a regulator that promotes plant growth at low temperature.
(2) The regulator for promoting the growth of plants at low temperature is used for tobacco plants, can promote the cold resistance of the tobacco plants, further remarkably promote photosynthesis, leaf area increase and biomass increase of the tobacco, and finally strengthen seedling quality of the tobacco.
Drawings
FIG. 1 is a graph showing the relative conductivity of a-2℃to 0℃low temperature treatment for 6 hours after three days of continuous spraying of 2, 3-dimercaptosuccinic acid solutions of different concentrations;
FIG. 2 is a graph showing malondialdehyde content of a-2℃to 0℃low temperature treatment for 6 hours after three days of continuous spraying of 2, 3-dimercaptosuccinic acid and surfactant solutions of different concentrations;
FIG. 3 is a graph of tobacco biomass continuously grown for 1 week after three days of low temperature treatment at-2℃to 0℃for 6 hours after continuous spraying of 2, 3-dimercaptosuccinic acid and surfactant solutions of different concentrations;
FIG. 4 is a graph of net photosynthetic rate of continued growth for 1 week after three days of-2℃to 0℃low temperature treatment for 6 hours following continuous spraying of 2, 3-dimercaptosuccinic acid at different concentrations;
FIG. 5 is a phenotype graph of tobacco grown continuously for 1 week after three days of low temperature treatment at-2℃to 0℃for 6 hours after continuous spraying of 2, 3-dimercaptosuccinic acid and surfactant solutions of different concentrations;
FIG. 6 is a graph of relative conductivity of a low temperature treatment of-2℃to 0℃for 6 hours after three days of continuous spraying of different concentrations of 2, 3-dimercaptosuccinic acid composition;
FIG. 7 is a graph showing malondialdehyde content of a-2℃to 0℃low temperature treatment for 6 hours after three days of continuous spraying of 2, 3-dimercaptosuccinic acid compositions of different concentrations;
FIG. 8 is a graph of tobacco aerial parts biomass grown continuously for 1 week after three days of-2℃to 0℃low temperature treatment for 6 hours after continuous spraying of 2, 3-dimercaptosuccinic acid compositions of different concentrations;
FIG. 9 is a graph of net photosynthetic rate of continued growth for 1 week after three days of-2℃to 0℃low temperature treatment for 6 hours following continuous spraying of 2, 3-dimercaptosuccinic acid compositions of varying concentrations;
FIG. 10 is a phenotype graph of tobacco grown continuously for 1 week after three days of-2℃to 0℃low temperature treatment for 6 hours after continuous spraying of 2, 3-dimercaptosuccinic acid compositions of different concentrations.
Detailed Description
The technical scheme of the application is further described below with reference to the accompanying drawings.
Example 1 preparation of regulators for promoting plant growth at Low temperatures-different concentrations of 2, 3-dimercaptosuccinic acid and surfactants
6mM 2, 3-dimercaptosuccinic acid: 1.093g of 2, 3-dimercaptosuccinic acid was weighed out and dissolved in 700 ml of water, the pH was adjusted to neutral, and the volume was fixed in a 1L volumetric flask.
6mM 2, 3-dimercaptosuccinic acid+surfactant: 1.093g of 2, 3-dimercaptosuccinic acid was weighed out and dissolved in 700 mL of water, 3mL of surfactant Triton X-100 was added, the pH was adjusted to neutral, and the volume was fixed in a 1L volumetric flask.
8mM2, 3-dimercaptosuccinic acid+surfactant: 1.458g of 2, 3-dimercaptosuccinic acid was weighed out and dissolved in 700 mL of water, 3mL of surfactant Triton X-100 was added, the pH was adjusted to neutral, and the volume was fixed in a 1L volumetric flask.
10mM2, 3-dimercaptosuccinic acid+surfactant: 1.822g of 2, 3-dimercaptosuccinic acid was weighed out and dissolved in 700 mL of water, 3mL of surfactant Triton X-100 was added, the pH was adjusted to be neutral, and the volume was fixed in a 1L volumetric flask.
13mM 2, 3-dimercaptosuccinic acid+surfactant: 2.369g of 2, 3-dimercaptosuccinic acid are weighed out and dissolved in 700 mL of water, 3mL of surfactant Triton X-100 are added, the pH is adjusted to be neutral, and the volume is fixed in a 1L volumetric flask.
Example 2 preparation of compositions of 2, 3-dimercaptosuccinic acid and glutamine as regulators for promoting Low temperature growth of plants
2, 3-dimercaptosuccinic acid composition 1 (6 mM2, 3-dimercaptosuccinic acid+1.5 mM glutamine mixed solution): 1.093g of 2, 3-dimercaptosuccinic acid and 0.2192g of glutamine were weighed out respectively, dissolved in 700 mL of water, and 3mL of surfactant Triton X-100 was added to adjust the pH to neutral, and the volume was fixed in a 1L volumetric flask.
2, 3-dimercaptosuccinic acid composition 2 (8 mM2, 3-dimercaptosuccinic acid+1.5 mM glutamine mixed solution): 1.458g of 2, 3-dimercaptosuccinic acid and 0.2192g of glutamine were weighed out respectively, dissolved in 700 mL of water, added with 3mL of surfactant Triton X-100, and adjusted to pH to neutral, and fixed in a 1L volumetric flask.
2, 3-dimercaptosuccinic acid composition 3 (10 mM2, 3-dimercaptosuccinic acid+1.5 mM glutamine mixed solution): 1.822g of 2, 3-dimercaptosuccinic acid and 0.2192g of glutamine were weighed, dissolved in 700 mL of water, and 3mL of surfactant Triton X-100 was added to adjust the pH to neutral, and the volume was fixed in a 1L volumetric flask.
Example 3 blade spraying
The regulator for promoting plant growth at low temperature, which is prepared in example 1, is added into a watering can, namely solutions of 2, 3-dimercaptosuccinic acid (the concentrations are 6mM, 8mM, 10mM and 13mM respectively) and a surfactant, and then the solutions are uniformly sprayed on the front and the back of tobacco (variety K326) leaves, wherein the spraying volume is 2 milliliters per plant, the control group is clear water with the same volume, and the low temperature (-2 ℃ to 0 ℃) treatment is carried out after three days of continuous spraying. The tobacco plants tested after the end of the low temperature treatment are divided into two parts, one of which is immediately subjected to the relative conductivity indicating the extent of damage to the cell membrane system and to the membrane peroxidation product malondialdehyde, see in particular examples 4 to 5. The experimental method mainly refers to the principle and technology of plant physiology and biochemistry which are mainly compiled by Wang Xuekui "
(2006, version 2, higher education Press). The other part was transferred to room temperature for further culture for one week, and the growth of the test plants was observed.
Example 4 determination of relative conductivity indicating the extent of injury to the cell membrane System
Treatment of the blade: spraying 2, 3-dimercaptosuccinic acid solution and surfactant solution in different concentration and low temperature treatment at-2-0 deg.cCleaning dirt on the surface of the blade after 6 hours, washing for 1-2 times by deionized water, sucking the water on the surface of the blade by clean gauze, obtaining round blades (avoiding main veins) by a blade puncher, and obtaining the blade area of about 8cm 2 。
10 groups of the solution are randomly taken and respectively put into a beaker with 10mL of deionized water to be soaked for 12 hours, and the conductivity of the solution in the beaker is measured and recorded as R1; the beaker was then placed in a boiling water bath and heated for 30 minutes, after which the conductivity of the solution in the beaker was measured again after leaving to stand at room temperature and noted as R2. The relative conductivity was calculated according to equation (1):
relative conductivity = R1/r2×100% (1)
The results of the relative conductivity measurements are shown in FIG. 1, which is a graph of the relative conductivity of the solution subjected to continuous spraying of 2, 3-dimercaptosuccinic acid and surfactant at-2℃for three days and then low-temperature treatment at 0℃for 6 hours, wherein C1-0 is the concentration of 2, 3-dimercaptosuccinic acid sprayed 6mM, C1-C4 is the concentration of 2, 3-dimercaptosuccinic acid sprayed with the surfactant solution, C1 is 6mM, C2 is 8mM, C3 is 10mM, and C4 is 13mM. As can be seen from fig. 1, the relative conductivity of the leaf sprayed with 6mM 2, 3-dimercaptosuccinic acid concentration alone tended to decrease, but the difference did not reach a significant level, as compared with the spraying of clear water; compared with the spraying of clear water, the relative conductivities of the blades sprayed with the 2, 3-dimercaptosuccinic acid with the concentration of 6mM, 8 and 10mM and the surfactant solution are all obviously reduced, which indicates that the tobacco blades sprayed with the 2, 3-dimercaptosuccinic acid with the concentration of 6-10mM have little damage to cells, and the more obvious the relative conductivities of the blades are reduced, the better the effect is; the difference in relative conductivity between the blades sprayed with the 2, 3-dimercaptosuccinic acid at a concentration of 13mM and the surfactant solution was not significant compared with the sprayed water, which further suggests that the spraying of the 2, 3-dimercaptosuccinic acid solution at a concentration of 6-10mM and the surfactant solution was the best.
Example 5 determination of the membranous peroxidation product malondialdehyde, which is an indication of the extent of injury to the cell membrane system
A phosphate buffer (pH 7.8) was prepared at 0.05mol/L, and a 0.5% thiobarbituric acid solution was prepared with a 5% trioxyacetic acid solution.
0.5g of tobacco leaves treated in accordance with example 4 were weighed separately, 2mL of precooled 0.05mol/L phosphate buffer (pH 7.8) and a small amount of quartz sand were added to each, homogenized in a precooled mortar, transferred to a 10mL centrifuge tube, the mortar was rinsed with phosphate buffer, the rinsing solution was also transferred to the centrifuge tube, and finally added to 5mL with phosphate buffer. Centrifuge for 10min at 4500 r/min. The supernatant fluid is malondialdehyde extract.
Respectively sucking 2mL of malondialdehyde extract into graduated test tubes, adding 3mL of 0.5% thiobarbituric acid solution, heating in boiling water bath for 10min, and rapidly cooling. Centrifuge in a centrifuge for 10min at 4500 r/min. The supernatant was taken and light transmittance was adjusted to 100% by using distilled water as a blank at wavelengths of 532nm and 600nm on a spectrophotometer, and absorbance was measured. Calculating the malondialdehyde content according to the formula (2):
wherein A: absorbance, V1: total reaction solution (5 mL), V: total extract (5 mL), V2: volume of extract in reaction solution (2 mL), W: plant sample weight (0.5 g).
The results of the malondialdehyde content are shown in FIG. 2, wherein FIG. 2 shows malondialdehyde content after three days of continuous spraying of solutions of 2, 3-dimercaptosuccinic acid and surfactant at different concentrations, C1-0 is spraying of 2, 3-dimercaptosuccinic acid at 6mM, C1-C4 is spraying of 2, 3-dimercaptosuccinic acid and surfactant solution, C1 is spraying of 2, 3-dimercaptosuccinic acid at 6mM, C2 is at 8mM, C3 is at 10mM, and C4 is at 13mM. As can be seen from fig. 2, the malondialdehyde content of leaves sprayed with 6mM 2, 3-dimercaptosuccinic acid concentration alone tended to decrease, but the difference did not reach a significant level, compared to the treatment sprayed with water; compared with the spraying of clear water, the malondialdehyde content of the leaves sprayed with the 2, 3-dimercaptosuccinic acid concentration of 6mM, 8 and 10mM and the surfactant solution is obviously reduced, but the malondialdehyde content of the leaves sprayed with the 2, 3-dimercaptosuccinic acid concentration of 13mM and the surfactant solution is not obviously different from that of the clear water treatment, which shows that the spraying of the solution with the 2, 3-dimercaptosuccinic acid concentration of 6-10mM and the surfactant has strong cold resistance and can obviously promote plant growth, can protect tobacco leaf cells from being damaged, and the more obviously the malondialdehyde content in the leaves is reduced along with the increase of the spraying concentration of the 2, 3-dimercaptosuccinic acid, the better the effect.
EXAMPLE 6 continuous spraying of a regulator for promoting plant growth at Low temperature- -solutions of 2, 3-dimercaptosuccinic acid and surfactant at different concentrations after three days- -Low temperature treatment at 0℃for 6h, and then transferring to a Artificial climatic chamber for continuous cultivation for one week
The growth of tobacco plants was observed after the continuous spraying of the regulator for promoting plant growth at low temperature, i.e., the solution of 2, 3-dimercaptosuccinic acid and surfactant at different concentrations for three days, was moved to a climatic chamber for continuous cultivation for 6 hours at-2℃to 0℃and then the growth of tobacco plants was observed, FIG. 3 is a graph of tobacco biomass continuously grown for 1 week after the continuous spraying of the solution of 2, 3-dimercaptosuccinic acid and surfactant at different concentrations for three days, i.e., the low temperature treatment at-2℃to 0℃and then the continuous spraying of the solution of 2, 3-dimercaptosuccinic acid and surfactant for 6mM, C1 to C4 was 2, 3-dimercaptosuccinic acid and surfactant was sprayed, wherein the concentration of 2, 3-dimercaptosuccinic acid was 6mM, the concentration of C2 was 8mM, the concentration of C3 was 10mM, and the concentration of C4 was 13mM. As can be seen from fig. 3, there is an upward trend in tobacco aerial biomass when sprayed with 6mM 2, 3-dimercaptosuccinic acid concentration alone, but the difference does not reach a significant level, as compared with the treatment when sprayed with water; tobacco aerial biomass sprayed with 2, 3-dimercaptosuccinic acid concentration of 6mM, 8 and 10mM and surfactant solution was significantly increased compared with that sprayed with clear water, but tobacco aerial biomass sprayed with 2, 3-dimercaptosuccinic acid concentration of 13mM and surfactant solution was not significantly different from that of clear water treatment, which suggests that spraying of 2, 3-dimercaptosuccinic acid concentration of 6-10mM and surfactant solution was not only strong in cold resistance but also significantly promoted plant growth, and the more significant the increase of tobacco aerial biomass with increasing of 2, 3-dimercaptosuccinic acid spray concentration was, the better the effect (FIG. 5).
EXAMPLE 7 determination of the net photosynthetic Rate of tobacco plants after continuous spraying of the regulator for promoting plant growth at Low temperature- -solutions of 2, 3-dimercaptosuccinic acid and surfactant at different concentrations for three days followed by continued treatment at-2℃to 0℃for 6h and then transfer to a Artificial climatic Chamber for continued cultivation for one week
The regulator for promoting plant growth at low temperature, namely the solution of 2, 3-dimercaptosuccinic acid with different concentrations and the surfactant, is continuously sprayed in the embodiment 3, tobacco plants treated for 6 hours at low temperature (-2-0 ℃) are continuously moved to a climatic chamber for cultivation for one week, 9:00-11:00 am with sufficient illumination is selected, and a LI-6800 photosynthetic apparatus is used for measuring the net photosynthetic rate. FIG. 4 is a graph of net photosynthetic rate of continued growth for 1 week by spraying solutions of 2, 3-dimercaptosuccinic acid and surfactant at different concentrations for three days, followed by a low temperature treatment at-2℃to 0℃for 6 hours, wherein C1-0 is the spraying of the 2, 3-dimercaptosuccinic acid at 6mM, C1-C4 is the spraying of the 2, 3-dimercaptosuccinic acid and surfactant solution, wherein the 2, 3-dimercaptosuccinic acid concentration C1 is 6mM, C2 is 8mM, C3 is 10mM, and C4 is 13mM. As can be seen from fig. 4, the net photosynthetic rate of the leaves sprayed with 6mM 2, 3-dimercaptosuccinic acid alone tended to increase, but did not reach the differential level, compared to the treatment sprayed with water; the net photosynthetic rate of leaves sprayed with 2, 3-dimercaptosuccinic acid concentration 6mM, 8mM and 10mM and surfactant solution was significantly increased compared to the treatment sprayed with water, but the difference in biomass of the aerial parts of tobacco sprayed with 2, 3-dimercaptosuccinic acid concentration 13mM and surfactant solution was not significant compared to the treatment with water. This shows that the blade is less damaged by cold damage at low temperature after spraying the solution of 2, 3-dimercaptosuccinic acid with the concentration of 6-10mM and the surfactant.
EXAMPLE 8 spraying of a regulator- -2, 3-dimercaptosuccinic acid composition for promoting plant growth at Low temperature
Experimental procedure the same as in example 3, different concentrations of the plant growth promoting regulator- -2, 3-dimercaptosuccinic acid composition 1, 2, 3-dimercaptosuccinic acid composition 2 and 2, 3-dimercaptosuccinic acid composition 3 configured in example 2 were sprayed on the front and back sides of different tobacco leaves, respectively, while a control group was set: spraying clear water and 10mM2, 3-dimercaptosuccinic acid and surfactant. The cold-resistant effect of the test low-temperature treatment is detected by continuously spraying for three days, then carrying out low-temperature treatment for 6 hours at the temperature of-2 ℃ to 0 ℃, dividing the tobacco plants to be tested into two parts after the low-temperature treatment for 6 hours at the temperature of-2 ℃ to 0 ℃, and immediately carrying out relative conductivity indicating the injury degree of a cell membrane system and a membrane peroxidation product malondialdehyde on one part, wherein the cold-resistant effect of the test low-temperature treatment is detected, and the specific examples are shown in examples 9 to 12. The test method mainly refers to the principle and technology of plant physiology and biochemistry experiments (2006, 2 nd edition, higher education press) which are mainly compiled by Wang Xuekui. Transferring the other part to a artificial climate chamber for culturing for one week, and observing the growth condition of the tested plant
Example 9 determination of relative conductivity indicating extent of injury to the cell membrane System after continuous spraying of modulators that promote plant growth at Low temperatures- -different concentrations of 2, 3-dimercaptosuccinic acid compositions
The tobacco plants tested were the tobacco plants treated in example 11, the leaf treatment and experimental procedure were the same as in example 5, 5 treatments were simultaneously applied, water (control) was sprayed, and a was 10mm2, 3-dimercaptosuccinic acid and surfactant; combination 1 was 2, 3-dimercaptosuccinic acid composition 1 (6 mM2, 3-dimercaptosuccinic acid+1.5 mM glutamine+surfactant); combination 2 was 2, 3-dimercaptosuccinic acid composition 2 (8 mM2, 3-dimercaptosuccinic acid+1.5 mM glutamine+surfactant); combination 3 was 2, 3-dimercaptosuccinic acid composition 3 (10 mM2, 3-dimercaptosuccinic acid+1.5 mM glutamine+surfactant). FIG. 6 is a graph showing the relative conductivity of the 2, 3-dimercaptosuccinic acid composition and other control materials after being continuously sprayed for three days at-2-0 ℃ for 6 hours, and as can be seen from FIG. 6, the relative conductivity of tobacco leaves can be obviously reduced by singly spraying the 2, 3-dimercaptosuccinic acid composition compared with the control clear water treatment, and the effect of spraying the 2, 3-dimercaptosuccinic acid composition is better than that of singly spraying the 2, 3-dimercaptosuccinic acid, so that the 2, 3-dimercaptosuccinic acid and glutamine play a synergistic effect.
EXAMPLE 10 determination of malondialdehyde, a membranous peroxidation product indicating the extent of injury to the cell membrane System after successive spraying of a regulator promoting plant growth at Low temperatures- -different concentrations of 2, 3-dimercaptosuccinic acid composition
The tobacco plants tested were tobacco plants treated in example 11, the leaf treatment was carried out in the same manner as in example 3, the experimental procedure was carried out in the same manner as in example 5, 5 treatments were simultaneously carried out, water (control) was sprayed, and A was 10mM2, 3-dimercaptosuccinic acid and surfactant; combination 1 was 2, 3-dimercaptosuccinic acid composition 1 (6 mM2, 3-dimercaptosuccinic acid+1.5 mM glutamine+surfactant); combination 2 was 2, 3-dimercaptosuccinic acid composition 2 (8 mM2, 3-dimercaptosuccinic acid+1.5 mM glutamine+surfactant); combination 3 was 2, 3-dimercaptosuccinic acid composition 3 (10 mM2, 3-dimercaptosuccinic acid+1.5 mM glutamine+surfactant). FIG. 7 is a graph showing malondialdehyde content of 2, 3-dimercaptosuccinic acid compositions of different concentrations after three days of low temperature treatment at-2℃to 0℃for 6 hours. As can be seen from fig. 7, the spraying of 2, 3-dimercaptosuccinic acid (a) alone significantly reduced the malondialdehyde content of the tobacco leaves compared to the control fresh water treatment, while the spraying of the 2, 3-dimercaptosuccinic acid composition was superior to the spraying of 2, 3-dimercaptosuccinic acid alone, indicating that the 2, 3-dimercaptosuccinic acid and glutamine have a synergistic effect.
EXAMPLE 11 continuous spraying of a modifier for promoting plant growth at Low temperature-2, 3-dimercaptosuccinic acid compositions at different concentrations-2-0 ℃ Low temperature treatment 6h after three days-2-0 ℃ continuous growth of tobacco plants for 1 week
Transferring another part of the tobacco plant in the example 8 to a climatic chamber for continuous culture for one week, observing the growth condition of the tested plant, simultaneously setting 5 treatments, spraying clear water (contrast), wherein A is 10mM2, 3-dimercaptosuccinic acid and surfactant; combination 1 was 2, 3-dimercaptosuccinic acid composition 1 (6 mM2, 3-dimercaptosuccinic acid+1.5 mM glutamine+surfactant); combination 2 was 2, 3-dimercaptosuccinic acid composition 2 (8 mM2, 3-dimercaptosuccinic acid+1.5 mM glutamine+surfactant); combination 3 was 2, 3-dimercaptosuccinic acid composition 3 (10 mM2, 3-dimercaptosuccinic acid+1.5 mM glutamine+surfactant). FIG. 8 is a chart of biomass of tobacco plants continuously sprayed with 2, 3-dimercaptosuccinic acid compositions of different concentrations for three days, and then subjected to low-temperature treatment at-2-0 ℃ for 6 hours to continue growing for 1 week. As can be seen from fig. 8, the spraying of 2, 3-dimercaptosuccinic acid (a) alone significantly promoted the increase in biomass in the aerial parts of tobacco compared to the control fresh water treatment, whereas the spraying of the 2, 3-dimercaptosuccinic acid composition was superior to the spraying of 2, 3-dimercaptosuccinic acid alone, indicating that the 2, 3-dimercaptosuccinic acid and glutamine gave a synergistic effect.
EXAMPLE 12 determination of the net photosynthetic Rate of tobacco plants after continuous spraying of regulators promoting plant growth at Low temperature- -2, 3-dimercaptosuccinic acid compositions of different concentrations for three days followed by continued treatment at-2℃to 0℃for 6h and then transfer to a Artificial climatic chamber for continued cultivation for one week
The tobacco plants tested were the tobacco plants treated in example 8, and the experimental procedure was the same as in example 7, with 5 treatments being applied with water (control), a being 10mm2, 3-dimercaptosuccinic acid and surfactant; combination 1 was 2, 3-dimercaptosuccinic acid composition 1 (6 mM2, 3-dimercaptosuccinic acid+1.5 mM glutamine+surfactant); combination 2 was 2, 3-dimercaptosuccinic acid composition 2 (8 mM2, 3-dimercaptosuccinic acid+1.5 mM glutamine+surfactant); combination 3 was 2, 3-dimercaptosuccinic acid composition 3 (10 mM2, 3-dimercaptosuccinic acid+1.5 mM glutamine+surfactant). FIG. 9 shows a graph of net photosynthetic rate of continued growth for 1 week after three days of-2℃to 0℃low temperature treatment for 6 hours following continuous spraying of different concentrations of 2, 3-dimercaptosuccinic acid composition. As can be seen from fig. 9, the spraying of 2, 3-dimercaptosuccinic acid (a) alone significantly improved the net photosynthetic rate of the tobacco leaves compared to the control fresh water treatment, while the spraying of the 2, 3-dimercaptosuccinic acid composition was superior to the spraying of 2, 3-dimercaptosuccinic acid alone, indicating that the 2, 3-dimercaptosuccinic acid and glutamine had a synergistic effect.
The foregoing description is only a few specific embodiments of the present application (the embodiments are not intended to be exhaustive, and the scope of the application includes the numerical range and other technical gist of the present application), and the details or common sense of the present application are not described in any detail herein. It should be noted that the above embodiments do not limit the present application in any way, and it is within the scope of the present application for those skilled in the art to obtain the technical solution by equivalent substitution or equivalent transformation. The protection scope of the present application is subject to the content of the claims, and the description of the specific embodiments and the like in the specification can be used for explaining the content of the claims.
Claims (9)
1. The application of a regulator comprising 2, 3-dimercaptosuccinic acid in low-temperature cold resistance of tobacco is characterized in that the concentration of the 2, 3-dimercaptosuccinic acid is 6-10 mM.
2. The use according to claim 1, wherein the regulator comprises 2, 3-dimercaptosuccinic acid and the surfactant Triton X-100, the mass ratio of 2, 3-dimercaptosuccinic acid to the surfactant Triton X-100 being 109-182:300.
3. The use according to claim 1 or 2, wherein the modulator further comprises glutamine, the mass ratio of 2, 3-dimercaptosuccinic acid to glutamine being 1090-1820:219.
4. The use according to claim 2, wherein the preparation method of the regulator comprises the steps of: weighing 2, 3-dimercaptosuccinic acid, dissolving in water, adding surfactant Triton X-100, regulating pH to neutrality, and fixing volume in volumetric flask.
5. Use according to claim 3, characterized in that the preparation method of the regulator comprises the following steps: 2, 3-dimercaptosuccinic acid and glutamine are respectively weighed and dissolved in water, then a surfactant Triton X-100 is added, the pH value is regulated to be neutral, and the mixture is obtained in a volumetric flask for constant volume.
6. The use according to claim 1 or 2, wherein the conditioning agent is uniformly sprayed on the front and back sides of the tobacco leaf, and then subjected to low temperature conditions.
7. The use according to claim 3, wherein the conditioning agent is uniformly sprayed on the front and back sides of the tobacco lamina and then subjected to low temperature conditions.
8. The use according to claim 6, wherein the low temperature condition is-2 ℃ to 0 ℃.
9. The use according to claim 7, wherein the low temperature condition is-2 ℃ to 0 ℃.
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| CN103392741A (en) * | 2013-07-02 | 2013-11-20 | 中国农业科学院农业资源与农业区划研究所 | Spraying agent for reducing cadmium absorption of tobacco in soil, and preparation and use methods thereof |
| CN107759289A (en) * | 2017-12-06 | 2018-03-06 | 农业部环境保护科研监测所 | The preparation method of the novel mercapto foliar fertilizer of cadmium content in a kind of reduction rice |
| CN113615691A (en) * | 2021-08-06 | 2021-11-09 | 南京农业大学 | Plant anti-refrigerant and preparation method and application thereof |
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| CN103392741A (en) * | 2013-07-02 | 2013-11-20 | 中国农业科学院农业资源与农业区划研究所 | Spraying agent for reducing cadmium absorption of tobacco in soil, and preparation and use methods thereof |
| CN107759289A (en) * | 2017-12-06 | 2018-03-06 | 农业部环境保护科研监测所 | The preparation method of the novel mercapto foliar fertilizer of cadmium content in a kind of reduction rice |
| CN113615691A (en) * | 2021-08-06 | 2021-11-09 | 南京农业大学 | Plant anti-refrigerant and preparation method and application thereof |
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