CN114903042A - Regulator for promoting growth of plants at low temperature, preparation method and application - Google Patents
Regulator for promoting growth of plants at low temperature, preparation method and application Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
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- A—HUMAN NECESSITIES
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- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
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Abstract
The invention discloses a regulator for promoting the growth of plants at low temperature, a preparation method and application thereof, wherein the regulator comprises a sulfhydryl compound 2, 3-dimercaptosuccinic acid, a surfactant and glutamine, the 2, 3-dimercaptosuccinic acid (or the 2, 3-dimercaptosuccinic acid and the glutamine) is dissolved in water, the surfactant Triton X-100 is added, and the pH value is regulated to be neutral to obtain a mixed solution. After the regulator for promoting the growth of the plants at low temperature is respectively sprayed on the front and back surfaces of the leaf surfaces of the plants, the tolerance of the plants to low-temperature cold damage can be effectively improved at low temperature, so that the plants can have stronger growth vigor and photosynthetic capacity at lower temperature, and the regulator has good application prospect in plant production.
Description
Technical Field
The invention relates to a regulator for promoting plant growth at low temperature and application thereof, belonging to the technical field of planting.
Background
The cold damage at low temperature is one of the important factors influencing the normal growth and development of plants, leading to the reduction of the growth and the yield of the plants. The agricultural and forestry crop loss caused by low-temperature injury is up to hundreds of billions of yuan each year around the world. The harm of low temperature to crops mainly shows in the aspects of cell membrane permeability, permeation regulation, generation and elimination of active oxygen free radicals and the like.
In view of the serious harm of low temperature, especially late spring cold frost to agricultural production, how to improve the low temperature resistance of plants and enhance the frost resistance of the plants becomes the focus of attention of researchers. Early studies have focused on protecting crops from low temperature stress by improving external environmental conditions such as mulch film mulching, material mulching of straws, branches and leaves, and the like, and performing measures such as smoking and fogging. How to stimulate the low-temperature resistance of plants in view of the damage of low temperature to the growth of the plants and how to reduce the low-temperature frost of crops is an urgent problem to be solved in agricultural production. The application of exogenous substances to plants to improve the cold resistance of the plants is of great significance for improving crop production.
Disclosure of Invention
The purpose of the invention is as follows: in order to solve the problems of the prior art, the first object of the invention is to provide a regulator for promoting the growth of plants at low temperature, the second object of the invention is to provide a preparation method of the regulator for promoting the growth of plants at low temperature, and the third object of the invention is to provide the application of the regulator for promoting the growth of plants at low temperature in the low-temperature cold resistance of tobacco.
The invention is realized by adopting the following technical scheme.
The invention relates to a regulator for promoting the growth of plants at low temperature, which comprises 2, 3-dimercaptosuccinic acid with the concentration of 6-10 mM.
Further, the regulator for promoting the growth of the plants at the low temperature comprises 2, 3-dimercaptosuccinic acid and a surfactant, wherein the mass ratio of the 2, 3-dimercaptosuccinic acid to the surfactant Triton X-100 is about 109-182: 300.
Further, the regulator for promoting the growth of the plants at the low temperature also comprises glutamine, and the mass ratio of the 2, 3-dimercaptosuccinic acid to the glutamine is 1090-1820: 219.
Furthermore, the concentration of the 2, 3-dimercaptosuccinic acid is 6-10 mM.
The preparation method of the regulator comprises the following steps: weighing 2, 3-dimercaptosuccinic acid, dissolving in water, adding surfactant Triton X-100, adjusting pH to neutral, and bottling.
The preparation method of the regulator comprises the following steps: the method comprises the following steps: respectively weighing 2, 3-dimercaptosuccinic acid and glutamine, dissolving in water, adding surfactant Triton X-100, adjusting pH to neutrality, and bottling in volumetric flask.
In the preparation method of the regulator, the concentration of the 2, 3-dimercaptosuccinic acid is 6-10 mM.
The technical scheme of the invention comprises the application of the regulator in low-temperature cold resistance of tobacco.
The application of the invention comprises that the regulator is uniformly sprayed on the front and back surfaces of the tobacco leaves and then is placed under a low temperature condition.
The low temperature condition is-2-0 deg.c (including any value in the range of 0 deg.c, -1 deg.c, -2 deg.c) and the treatment period is 6 hr.
The cold-resistant mechanism of the invention: after the regulator for promoting the growth of the plants at the low temperature is sprayed, the cell membrane permeability of leaves at the low temperature is obviously reduced, the damage of the low temperature to the cell membrane can be reduced, and the photosynthetic capacity and biomass of the leaves of the tobacco plants are promoted to increase so as to promote the growth of the plants.
Has the advantages that: compared with the prior art, the invention has the following remarkable advantages:
(1) the invention provides a novel regulator for promoting the growth of plants at low temperature for the first time. The cold resistance of the plant is enhanced after the plant leaves are sprayed with the regulator for promoting the growth of the plant at low temperature.
(2) The regulator for promoting the growth of the plants at the low temperature is used for the tobacco plants, and can promote the cold resistance of the tobacco plants, so that the photosynthesis, the increase of the leaf area and the increase of the biomass of the tobacco are remarkably promoted, and finally the seedling quality of the tobacco is enhanced.
Drawings
FIG. 1 is a graph of the relative conductivity of 2, 3-dimercaptosuccinic acid of different concentrations and surfactant solution after continuous spraying for three days, after low-temperature treatment at-2 deg.C to 0 deg.C for 6 h;
FIG. 2 is a graph showing the malondialdehyde content after continuously spraying 2, 3-dimercaptosuccinic acid and surfactant solutions of different concentrations for three days and then carrying out low-temperature treatment at-2 ℃ to 0 ℃ for 6 hours;
FIG. 3 is a biological quantity diagram of tobacco continuously growing for 1 week after low-temperature treatment at-2 deg.C to 0 deg.C for 6h after continuously spraying 2, 3-dimercaptosuccinic acid and surfactant solution with different concentrations for three days;
FIG. 4 is a graph of net photosynthetic rate after continuous spraying of 2, 3-dimercaptosuccinic acid of different concentrations and a surfactant solution for three days, followed by low-temperature treatment at-2 ℃ to 0 ℃ for 6h and continued growth for 1 week;
FIG. 5 is a table diagram of tobacco growing for 1 week after continuously spraying 2, 3-dimercaptosuccinic acid with different concentrations and a surfactant solution for three days and then treating at low temperature of-2 ℃ to 0 ℃ for 6 hours;
FIG. 6 is a graph of the relative conductivities of 2, 3-dimercaptosuccinic acid compositions after continuous spraying for three days after low-temperature treatment at-2 ℃ to 0 ℃ for 6 hours;
FIG. 7 is a graph showing the malondialdehyde content after continuous spraying of 2, 3-dimercaptosuccinic acid compositions of different concentrations for three days and low-temperature treatment at-2 deg.C to 0 deg.C for 6 h;
FIG. 8 is a biological quantity chart of the overground parts of tobacco continuously growing for 1 week after low-temperature treatment at-2 ℃ to 0 ℃ for 6 hours after continuously spraying 2, 3-dimercaptosuccinic acid compositions with different concentrations for three days;
FIG. 9 is a graph of net photosynthetic rate after continuous spraying of 2, 3-dimercaptosuccinic acid compositions of different concentrations for three days, after low temperature treatment at-2 deg.C to 0 deg.C for 6h and continued growth for 1 week;
FIG. 10 is a table diagram of tobacco growing for 1 week after low-temperature treatment at-2 deg.C to 0 deg.C for 6h after continuously spraying 2, 3-dimercaptosuccinic acid compositions with different concentrations for three days.
Detailed Description
The technical scheme of the invention is further explained by combining the attached drawings.
EXAMPLE 1 preparation of the regulators for promoting the growth of plants at Low temperatures-2, 3-dimercaptosuccinic acid of various concentrations and surfactants
6mM 2, 3-dimercaptosuccinic acid: 1.093g of 2, 3-dimercaptosuccinic acid was weighed out and dissolved in 700 ml of water, the pH was adjusted to neutral, and the volume was determined in a 1L volumetric flask.
6mM 2, 3-dimercaptosuccinic acid + surfactant: weighing 1.093g of 2, 3-dimercaptosuccinic acid, dissolving in 700 mL of water, adding 3mL of surfactant Triton X-100, adjusting the pH value to be neutral, and fixing the volume in a 1L volumetric flask.
8mM2, 3-dimercaptosuccinic acid + surfactant: weighing 1.458g of 2, 3-dimercaptosuccinic acid, dissolving in 700 mL of water, adding 3mL of surfactant Triton X-100, adjusting the pH value to be neutral, and fixing the volume in a 1L volumetric flask.
10mM2, 3-dimercaptosuccinic acid + surfactant: 1.822g of 2, 3-dimercaptosuccinic acid is weighed, dissolved in 700 mL of water, 3mL of surfactant Triton X-100 is added, the pH is adjusted to be neutral, and the volume is determined in a 1L volumetric flask.
13mM 2, 3-dimercaptosuccinic acid + surfactant: 2.369g of 2, 3-dimercaptosuccinic acid is weighed and dissolved in 700 mL of water, 3mL of surfactant Triton X-100 is added, the pH value is adjusted to be neutral, and the volume is determined in a 1L volumetric flask.
EXAMPLE 2 preparation of a composition of 2, 3-dimercaptosuccinic acid and glutamine as a regulator for promoting growth of plants at Low temperatures
2, 3-dimercaptosuccinic acid composition 1 (mixed solution of 6mM 2, 3-dimercaptosuccinic acid +1.5mM glutamine): 1.093g of 2, 3-dimercaptosuccinic acid and 0.2192g of glutamine are weighed respectively and dissolved in 700 mL of water, 3mL of Triton X-100 as a surfactant is added, the pH value is adjusted to be neutral, and the volume is fixed in a 1L volumetric flask.
2, 3-dimercaptosuccinic acid composition 2 (mixed solution of 8mM2, 3-dimercaptosuccinic acid +1.5mM glutamine): 1.458g of 2, 3-dimercaptosuccinic acid and 0.2192g of glutamine are respectively weighed and dissolved in 700 mL of water, 3mL of surfactant Triton X-100 is added, the pH is adjusted to be neutral, and the volume is determined in a 1L volumetric flask.
2, 3-dimercaptosuccinic acid composition 3 (mixed solution of 10mM2, 3-dimercaptosuccinic acid +1.5mM glutamine): 1.822g of 2, 3-dimercaptosuccinic acid and 0.2192g of glutamine were weighed, dissolved in 700 mL of water, 3mL of surfactant Triton X-100 was added, the pH was adjusted to neutrality, and the volume was determined in a 1L volumetric flask.
Example 3 foliar spray application
The regulator for promoting the growth of the plants at low temperature, namely the 2, 3-dimercaptosuccinic acid with different concentrations (the concentrations are respectively 6mM, 8mM, 10mM and 13mM) and the solution of the surfactant, which are prepared in the embodiment 1, are respectively added into a spraying pot, then the solution is respectively and uniformly sprayed on the front and back surfaces of the leaves of the tobacco (variety K326), the spraying volume is 2ml for each plant, the control group is that the same volume of clear water is sprayed, and the low-temperature (-2 ℃ -0 ℃) treatment is carried out for 6 hours after the solution is continuously sprayed for three days. After the low-temperature treatment, the tobacco plants to be tested are divided into two parts, and one part is immediately subjected to the relative conductivity indicating the damage degree of the cell membrane system and the membranous peroxidation product malondialdehyde, which is specifically shown in examples 4 to 5. The test method mainly refers to the principle and technology of plant physiological and biochemical experiment compiled by Wangchi "
(2006 version 2, higher education publishers). The other part is transferred to room temperature and cultured for one week, and the growth condition of the test plants is observed.
Example 4 determination of relative conductivity indicating extent of damage to cell membrane systems
And (3) processing the blade: respectively cleaning the dirt on the surface of the blade after spraying 2, 3-dimercaptosuccinic acid and surfactant solutions with different concentrations in the embodiment 3 and carrying out low-temperature treatment at-2-0 ℃ for 6 hours, washing the blade with deionized water for 1-2 times, sucking the water on the surface of the blade by clean gauze, and then obtaining round blades (avoiding main vein) by using a blade puncher, wherein the area of each blade is about 8cm 2 。
Randomly taking 10 of each group, respectively putting the 10 groups into a beaker added with 10mL of deionized water, soaking for 12h, and measuring the conductivity of the solution in the beaker, wherein the conductivity is marked as R1; the beaker was then placed in a boiling water bath and heated for 30min, after which time the electrical conductivity of the solution in the beaker was measured and recorded as R2. The relative conductivity was calculated according to equation (1):
relative conductivity R1/R2X 100% (1)
The results of the relative conductivity measurement are shown in fig. 1, and fig. 1 is a relative conductivity graph of-2 ℃ to 0 ℃ low-temperature treatment for 6h after continuously spraying solutions of 2, 3-dimercaptosuccinic acid with different concentrations and a surfactant for three days, wherein C1-0 is spraying 2, 3-dimercaptosuccinic acid with a concentration of 6mM, C1-C4 is spraying 2, 3-dimercaptosuccinic acid with a concentration of 6mM, C2 is 8mM, C3 is 10mM, and C4 is 13 mM. As can be seen from FIG. 1, the relative conductivity of the leaves sprayed with 6mM 2, 3-dimercaptosuccinic acid alone has a tendency to decrease, but the difference does not reach a significant level, compared with the leaves sprayed with clear water; compared with spraying clear water, the leaf blade sprayed with the 2, 3-dimercaptosuccinic acid with the concentrations of 6mM, 8mM and 10mM and the surfactant solution has obviously reduced relative conductivity, which indicates that the tobacco leaf blade sprayed with the 2, 3-dimercaptosuccinic acid with the concentration of 6-10mM has small cell damage, and the more obvious the relative conductivity of the leaf blade is reduced along with the increase of the concentration of the 2, 3-dimercaptosuccinic acid, the better the effect is; compared with spraying clear water, the leaf blade sprayed with the 2, 3-dimercaptosuccinic acid solution with the concentration of 13mM and the surfactant solution has insignificant difference of the relative conductivity, which further indicates that the spraying effect of the 2, 3-dimercaptosuccinic acid solution with the concentration of 6-10mM and the surfactant solution is the best.
Example 5 determination of the membranous peroxidation product malondialdehyde as an indication of the extent of damage to the membrane systems of cells
0.05mol/L of phosphate buffer (pH7.8) is prepared, and a 0.5% thiobarbituric acid solution is prepared by using a 5% trioxyacetic acid solution.
0.5g of tobacco leaves treated according to example 4 were weighed out separately, 2mL of each of a precooled 0.05mol/L phosphate buffer (pH7.8) and a small amount of quartz sand were added, ground into a homogenate in a precooled mortar, transferred into a 10mL centrifuge tube, the mortar was washed with the phosphate buffer, the wash was also transferred into the centrifuge tube, and finally added to 5mL with the phosphate buffer. Centrifuging at 4500r/min for 10 min. The supernatant is the extracting solution of malonaldehyde.
Respectively sucking 2mL of malondialdehyde extractive solution into a graduated test tube, respectively adding 3mL of 0.5% thiobarbituric acid solution, heating in boiling water bath for 10min, and rapidly cooling. Centrifuging at 4500r/min for 10 min. Taking the supernatant, adjusting the light transmittance to 100% by using distilled water as a blank under the wavelength of 532nm and 600nm on a spectrophotometer, and measuring the absorbance. Calculating the malondialdehyde content according to formula (2):
wherein, A: absorbance, V1: total amount of reaction solution (5mL), V: total amount of extract (5mL), V2: volume of extract in reaction solution (2mL), W: plant sample weight (0.5 g).
The results of the malondialdehyde content are shown in fig. 2, and fig. 2 is a malondialdehyde content diagram of-2 ℃ to 0 ℃ low-temperature treatment for 6h after continuously spraying solutions of 2, 3-dimercaptosuccinic acid with different concentrations and a surfactant for three days, wherein C1-0 is spraying 2, 3-dimercaptosuccinic acid with the concentration of 6mM, C1-C4 is spraying 2, 3-dimercaptosuccinic acid and a surfactant solution, wherein the concentration of 2, 3-dimercaptosuccinic acid is C1 of 6mM, C2 of 8mM, C3 of 10mM, and C4 of 13 mM. As can be seen from FIG. 2, the malondialdehyde content of the leaf blade sprayed with 6mM 2, 3-dimercaptosuccinic acid alone has a tendency to decrease, but the difference does not reach a significant level, compared with the treatment of spraying clear water; compared with spraying clear water, the malonaldehyde content of the leaves sprayed with the 2, 3-dimercaptosuccinic acid solution with the concentrations of 6mM, 8mM and 10mM and the surfactant solution is remarkably reduced, but the malonaldehyde content of the leaves sprayed with the 2, 3-dimercaptosuccinic acid solution with the concentration of 13mM and the surfactant solution is not remarkably different from the treatment of the clear water, which shows that the 2, 3-dimercaptosuccinic acid solution with the concentration of 6-10mM and the surfactant solution is not only strong in cold resistance but also can remarkably promote plant growth and protect tobacco leaf cells from being damaged, and the more remarkable the malonaldehyde content in the leaves is reduced along with the increase of the spraying concentration of the 2, 3-dimercaptosuccinic acid, the better effect is achieved.
Example 6 growth of tobacco plants after continuous spraying of a solution of 2, 3-dimercaptosuccinic acid and a surfactant at different concentrations for three days and then low temperature treatment at-2 ℃ to 0 ℃ for 6h, followed by further cultivation in a phytotron for one week
The tobacco which is continuously sprayed with the regulator for promoting the growth of the plant at low temperature, namely the regulator with different concentrations of 2, 3-dimercaptosuccinic acid and the surfactant in the embodiment 3, is moved to an artificial climate chamber for low-temperature treatment at the temperature of-2 ℃ -0 ℃ for 6h after being continuously cultured for one week, and then the growth condition of the tobacco plant is observed, wherein a biological quantity diagram of the tobacco which is continuously sprayed with the regulator with different concentrations of 2, 3-dimercaptosuccinic acid and the surfactant for three days and then is continuously grown for 1 week after being treated at the temperature of-2 ℃ -0 ℃ for 6h is shown in figure 3, C1-0 is a tobacco biomass diagram, wherein the concentration of 2, 3-dimercaptosuccinic acid is 6mM, the concentration of 2, 3-dimercaptosuccinic acid and the surfactant solution is sprayed, and the concentration of 2, 3-dimercaptosuccinic acid and the surfactant solution is 1 mM, the concentration of C2 is 8mM, the concentration of C3 is 10mM, and the concentration of C4 is 13 mM. As can be seen from FIG. 3, compared with the treatment of spraying clear water, the biomass of the overground part of the tobacco sprayed with 6mM 2, 3-dimercaptosuccinic acid alone has a rising trend, but the difference does not reach a significant level; compared with spraying clear water, the biomass of the overground part of the tobacco sprayed with the 2, 3-dimercaptosuccinic acid solution with the concentrations of 6mM, 8mM and 10mM and the surfactant solution is remarkably increased, but the difference between the biomass of the overground part of the tobacco sprayed with the 2, 3-dimercaptosuccinic acid solution with the concentration of 13mM and the surfactant solution and the treatment of the clear water is not remarkable, which shows that the spraying of the 2, 3-dimercaptosuccinic acid solution with the concentration of 6-10mM and the surfactant solution has strong cold resistance and can remarkably promote the growth of plants, and the more remarkable the increase of the biomass of the overground part of the tobacco along with the increase of the spraying concentration of the 2, 3-dimercaptosuccinic acid, the better effect (figure 5).
Example 7 determination of net photosynthetic Rate of tobacco plants after continuous spraying of a solution of 2, 3-dimercaptosuccinic acid and a surfactant at different concentrations to promote growth of plants at Low temperature for three days, followed by continuous Low temperature treatment at-2 deg.C to 0 deg.C for 6h, and then moving to a phytotron to continue culturing for one week
After continuously spraying the regulator for promoting the growth of the plants at low temperature, namely 2, 3-dimercaptosuccinic acid with different concentrations and the solution of the surfactant, which are obtained in the embodiment 3, for three days, the tobacco plants are treated at low temperature (-2 ℃ -0 ℃) for 6h and then are continuously moved to an artificial climate chamber for culturing for one week, 9:00-11:00 in the morning with sufficient illumination is selected, and the net photosynthetic rate is determined by using an LI-6800 photosynthetic instrument. FIG. 4 is a graph of net photosynthetic rate after continuous spraying of solutions of 2, 3-dimercaptosuccinic acid and surfactant at different concentrations for three days, after low-temperature treatment at-2 deg.C to 0 deg.C for 6h and continued growth for 1 week, wherein C1-0 is sprayed with a concentration of 6mM of 2, 3-dimercaptosuccinic acid, C1-C4 is sprayed with a solution of 2, 3-dimercaptosuccinic acid and surfactant, wherein the concentration of 2, 3-dimercaptosuccinic acid C1 is 6mM, C2 is 8mM, C3 is 10mM, and C4 is 13 mM. As can be seen from FIG. 4, the net photosynthetic rate of leaves sprayed with 6mM 2, 3-dimercaptosuccinic acid alone tended to increase, but did not reach a different level, compared to the treatment of spraying with clear water; compared with the treatment of spraying clear water, the net photosynthetic rate of the leaves sprayed with the 2, 3-dimercaptosuccinic acid with the concentration of 6mM, 8mM and 10mM and the surfactant solution is obviously increased, but the difference between the biomass of the overground part of the tobacco sprayed with the 2, 3-dimercaptosuccinic acid with the concentration of 13mM and the surfactant solution and the clear water treatment is not obvious. This shows that the leaves are less damaged by low temperature cold injury after the solution of 2, 3-dimercaptosuccinic acid with the concentration of 6-10mM and the surfactant is sprayed.
Example 8 spraying of 2, 3-dimercaptosuccinic acid composition, a regulator for promoting the growth of plants at Low temperatures
The experimental procedure is the same as that in example 3, 2, 3-dimercaptosuccinic acid composition 1, 2, 3-dimercaptosuccinic acid composition 2 and 2, 3-dimercaptosuccinic acid composition 3, which are different concentrations of the regulators for promoting the growth of plants at low temperature, prepared in example 2 are respectively sprayed on the front and back sides of different tobacco leaves, and meanwhile, a control group is set: spraying clear water, and spraying 10mM2, 3-dimercapto succinic acid and a surfactant. After continuously spraying for three days, carrying out low-temperature treatment at-2-0 ℃ for 6h, and after finishing the low-temperature treatment at-2-0 ℃ for 6h, dividing the tobacco plant to be tested into two parts, and immediately carrying out relative conductivity indicating the damage degree of a cell membrane system and a membrane peroxidation product malondialdehyde on one part to detect the cold resistance effect of the low-temperature treatment of the test, which is specifically shown in examples 9-12. The test method mainly refers to the plant physiological and biochemical experiment principle and technology compiled by the King university Quiki (2006, 2 nd edition, higher education Press). Moving the other part to a phytotron to continue culturing for one week, and observing the growth condition of the test plants
Example 9 determination of relative conductivity indicating extent of cell membrane system injury after continuous spray application of 2, 3-dimercaptosuccinic acid compositions of varying concentrations as regulators for promoting plant growth at low temperatures
The tobacco plants to be tested were the tobacco plants treated in example 11, the leaf treatment mode and the experimental process were the same as in example 5, 5 treatments were set simultaneously, clean water (control) was sprayed, and a was 10mM2, 3-dimercaptosuccinic acid and a surfactant; combination 1 was 2, 3-dimercaptosuccinic acid composition 1(6mM 2, 3-dimercaptosuccinic acid +1.5mM glutamine + surfactant); combination 2 was 2, 3-dimercaptosuccinic acid composition 2(8mM2, 3-dimercaptosuccinic acid +1.5mM glutamine + surfactant); combination 3 was 2, 3-dimercaptosuccinic acid composition 3(10mM2, 3-dimercaptosuccinic acid +1.5mM glutamine + surfactant). FIG. 6 is a graph of the relative conductivity of 2, 3-dimercaptosuccinic acid compositions with different concentrations and other controls after continuously spraying for three days and then treating at-2 ℃ to 0 ℃ for 6 hours, and it can be seen from FIG. 6 that compared with the control clear water treatment, the effect of spraying 2, 3-dimercaptosuccinic acid (A) alone can significantly reduce the relative conductivity of tobacco leaves, and the effect of spraying 2, 3-dimercaptosuccinic acid composition is better than the effect of spraying 2, 3-dimercaptosuccinic acid alone, which shows that 2, 3-dimercaptosuccinic acid and glutamine have synergistic effect.
Example 10 determination of membranous peroxidation product malondialdehyde indicating extent of cellular membrane system injury after continuous spraying of 2, 3-dimercaptosuccinic acid compositions of varying concentrations as regulators for promoting plant growth at low temperatures
The tobacco plants to be tested were the tobacco plants treated in example 11, the leaf treatment was performed in the same manner as in example 3, the experimental procedure was the same as in example 5, 5 treatments were simultaneously performed, clean water (control) was sprayed, and a was 10mM2, 3-dimercaptosuccinic acid and a surfactant; combination 1 was 2, 3-dimercaptosuccinic acid composition 1(6mM 2, 3-dimercaptosuccinic acid +1.5mM glutamine + surfactant); combination 2 was 2, 3-dimercaptosuccinic acid composition 2(8mM2, 3-dimercaptosuccinic acid +1.5mM glutamine + surfactant); combination 3 was 2, 3-dimercaptosuccinic acid composition 3(10mM2, 3-dimercaptosuccinic acid +1.5mM glutamine + surfactant). FIG. 7 is a graph showing the malondialdehyde content after low-temperature treatment at-2 deg.C to 0 deg.C for 6h after continuously spraying 2, 3-dimercaptosuccinic acid compositions of different concentrations for three days. As can be seen from FIG. 7, compared with the clear water treatment, the single spraying of 2, 3-dimercaptosuccinic acid (A) can significantly reduce the malondialdehyde content of tobacco leaves, and the effect of the spraying of the 2, 3-dimercaptosuccinic acid composition is superior to the effect of the single spraying of 2, 3-dimercaptosuccinic acid, which indicates that 2, 3-dimercaptosuccinic acid and glutamine have a synergistic effect.
Example 11 continuous spray application of a plant growth promoting regulator- -2, 3-dimercaptosuccinic acid compositions of different concentrations for three days followed by low temperature treatment at-2 deg.C to 0 deg.C for 6 hours to continue normal growth for 1 week
Another part of the tobacco plants in example 8 was transferred to a phytotron and cultured for a week, the growth of the test plants was observed, 5 treatments were set simultaneously, and clean water (control) was sprayed, a being 10mM2, 3-dimercaptosuccinic acid and a surfactant; combination 1 was 2, 3-dimercaptosuccinic acid composition 1(6mM 2, 3-dimercaptosuccinic acid +1.5mM glutamine + surfactant); combination 2 was 2, 3-dimercaptosuccinic acid composition 2(8mM2, 3-dimercaptosuccinic acid +1.5mM glutamine + surfactant); combination 3 was 2, 3-dimercaptosuccinic acid composition 3(10mM2, 3-dimercaptosuccinic acid +1.5mM glutamine + surfactant). FIG. 8 is a graph of the biomass of tobacco plants grown for 1 week after continuous spraying of 2, 3-dimercaptosuccinic acid compositions of different concentrations for three days, low temperature treatment at-2 deg.C to 0 deg.C for 6h, and continued growth. As can be seen from FIG. 8, compared with the control clear water treatment, the spraying of 2, 3-dimercaptosuccinic acid (A) alone can significantly promote the biomass increase of the overground part of the tobacco, and the effect of spraying the 2, 3-dimercaptosuccinic acid composition is better than that of spraying 2, 3-dimercaptosuccinic acid alone, which shows that 2, 3-dimercaptosuccinic acid and glutamine have a synergistic effect.
Example 12 determination of net photosynthetic Rate of tobacco plants after continuous spraying of a regulator for promoting growth of plants at Low temperature- -2, 3-dimercaptosuccinic acid compositions of different concentrations for three days, followed by continuous Low temperature treatment at-2 deg.C to 0 deg.C for 6h, and then moving to a phytotron for continuous cultivation for one week
The tobacco plants tested were the tobacco plants treated in example 8, the experimental procedure was the same as in example 7, 5 treatments were simultaneously performed, clean water (control) was sprayed, and a was 10mM2, 3-dimercaptosuccinic acid and surfactant; combination 1 was 2, 3-dimercaptosuccinic acid composition 1(6mM 2, 3-dimercaptosuccinic acid +1.5mM glutamine + surfactant); combination 2 was 2, 3-dimercaptosuccinic acid composition 2(8mM2, 3-dimercaptosuccinic acid +1.5mM glutamine + surfactant); combination 3 was 2, 3-dimercaptosuccinic acid composition 3(10mM2, 3-dimercaptosuccinic acid +1.5mM glutamine + surfactant). FIG. 9 is a graph of net photosynthetic rate after continuous spraying of 2, 3-dimercaptosuccinic acid compositions of different concentrations for three days, after low-temperature treatment at-2 deg.C to 0 deg.C for 6h and continued growth for 1 week. As can be seen from FIG. 9, compared with the control clear water treatment, the single spraying of 2, 3-dimercaptosuccinic acid (A) can significantly promote the increase of the net photosynthetic rate of tobacco leaves, and the effect of the single spraying of the 2, 3-dimercaptosuccinic acid composition is better than that of the single spraying of 2, 3-dimercaptosuccinic acid, which indicates that 2, 3-dimercaptosuccinic acid and glutamine play a synergistic role.
The above description is only a part of the specific embodiments of the present invention (since the present invention includes the numerical range, the embodiments are not exhaustive, the protection scope of the present invention includes the numerical range and other technical essential ranges), and the detailed contents or common general knowledge known in the schemes are not described too much. It should be noted that the above-mentioned embodiments do not limit the present invention in any way, and all technical solutions obtained by means of equivalent substitution or equivalent transformation for those skilled in the art are within the protection scope of the present invention. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (10)
1. A regulator for promoting the growth of plants at low temperature is characterized by comprising 2, 3-dimercaptosuccinic acid with the concentration of 6-10 mM.
2. The plant growth regulator according to claim 1, wherein the plant growth regulator comprises 2, 3-dimercaptosuccinic acid and a surfactant, and the mass ratio of the 2, 3-dimercaptosuccinic acid to the surfactant Triton X-100 is about 109-182: 300.
3. The regulator for promoting the growth of plants at low temperature as claimed in claim 1 or 2, wherein the regulator for promoting the growth of plants at low temperature further comprises glutamine, and the mass ratio of the 2, 3-dimercaptosuccinic acid to the glutamine is 1090-1820: 219.
4. The modulator according to claim 2 or 3, wherein the concentration of 2, 3-dimercaptosuccinic acid is 6-10 mM.
5. The method for preparing a regulator for promoting plant growth at low temperature according to claim 2, comprising the steps of: weighing 2, 3-dimercaptosuccinic acid, dissolving in water, adding surfactant Triton X-100, adjusting pH to neutral, and bottling.
6. The method for preparing a regulator for promoting the growth of plants at low temperature according to claim 3, comprising the steps of: respectively weighing 2, 3-dimercaptosuccinic acid and glutamine, dissolving in water, adding surfactant Triton X-100, adjusting pH to neutrality, and bottling in volumetric flask.
7. The method for preparing the regulator according to claim 5 or 6, wherein the concentration of the 2, 3-dimercaptosuccinic acid is 6 to 10 mM.
8. Use of the modulator of any one of claims 1 to 4 for the low temperature cold resistance of tobacco.
9. The use of claim 8, wherein said use comprises applying said conditioning agent uniformly to the front and back sides of tobacco lamina prior to exposure to cryogenic conditions.
10. Use according to claim 9, wherein said cryogenic conditions are-2 ℃ to 0 ℃.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103392741A (en) * | 2013-07-02 | 2013-11-20 | 中国农业科学院农业资源与农业区划研究所 | Spraying agent for reducing cadmium absorption of tobacco in soil, and preparation and use methods thereof |
| CN107759289A (en) * | 2017-12-06 | 2018-03-06 | 农业部环境保护科研监测所 | The preparation method of the novel mercapto foliar fertilizer of cadmium content in a kind of reduction rice |
| US20200329710A1 (en) * | 2018-01-15 | 2020-10-22 | Locus Agriculture Ip Company, Llc | Materials and Methods for Treating Bacterial Infections in Plants |
| CN113615691A (en) * | 2021-08-06 | 2021-11-09 | 南京农业大学 | Plant anti-refrigerant and preparation method and application thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392741A (en) * | 2013-07-02 | 2013-11-20 | 中国农业科学院农业资源与农业区划研究所 | Spraying agent for reducing cadmium absorption of tobacco in soil, and preparation and use methods thereof |
| CN107759289A (en) * | 2017-12-06 | 2018-03-06 | 农业部环境保护科研监测所 | The preparation method of the novel mercapto foliar fertilizer of cadmium content in a kind of reduction rice |
| US20200329710A1 (en) * | 2018-01-15 | 2020-10-22 | Locus Agriculture Ip Company, Llc | Materials and Methods for Treating Bacterial Infections in Plants |
| CN113615691A (en) * | 2021-08-06 | 2021-11-09 | 南京农业大学 | Plant anti-refrigerant and preparation method and application thereof |
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