CN114794112B - Anti-transpiration agent containing S-abscisic acid and preparation method thereof - Google Patents

Anti-transpiration agent containing S-abscisic acid and preparation method thereof Download PDF

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CN114794112B
CN114794112B CN202110122537.5A CN202110122537A CN114794112B CN 114794112 B CN114794112 B CN 114794112B CN 202110122537 A CN202110122537 A CN 202110122537A CN 114794112 B CN114794112 B CN 114794112B
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transpiration
pinolene
polyoxyethylene ether
oil
abscisic acid
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CN114794112A (en
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吴成林
李达忠
叶宇平
杨建江
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Foshan Hailiying Biotechnology Co ltd
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Foshan Hailiying Biotechnology Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/42Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A01N25/04Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention belongs to the field of anti-transpiration preparations, and in particular relates to an anti-transpiration agent containing S-abscisic acid and a preparation method thereof. The anti-transpiration agent comprises the following components in percentage by mass based on 100% of the total mass of the anti-transpiration agent: s-elicitor 1% -20%; 1% -80% of pinolene; the balance being excipient. Mixing S-abscisic acid, pinolene and excipient, stirring, and homogenizing. The S-abscisic acid and the pinolene have synergistic transpiration resistance and insect disease resistance, have obvious transpiration resistance, can increase the water potential of plant leaves and fruits, improve the crop yield, improve the plant transplanting and cutting survival rate, heat and preserve moisture, resist insect disease, improve plant resistance and the like, and can be widely applied to the fields of ornamental greening tree species, big tree transplanting, fruit, vegetable, flower preservation and the like. Has the advantages of high safety, excellent wetting and spreading performance, good film forming property and the like.

Description

Anti-transpiration agent containing S-abscisic acid and preparation method thereof
Technical Field
The invention belongs to the field of anti-transpiration preparations, and in particular relates to an anti-transpiration agent containing S-abscisic acid and a preparation method thereof.
Background
The S-elicitor can play a bacteriostatic role by activating an immune system and a defense mechanism of a plant, promote callose formation in an infection area, protect crop tissues from pathogen and bacterial infection, promote crops from being affected by some bacterial and fungal diseases under the condition of generating resistance, regulate the closure of air holes of crop leaves, prevent pathogen invasion, block pathogen invasion paths, reduce the incidence rate and slow down the transpiration. Besides disease resistance, the fertilizer also has the function of promoting the absorption of nutrient elements such as potassium, calcium, magnesium, phosphorus and the like by crops.
The anti-transpiration agent is a chemical substance which acts on the surface of plant leaves and can reduce the transpiration intensity and reduce the water loss. The anti-transpiration agent is a novel drought-resistant control technology and plays an important role in drought resistance, water conservation, disaster reduction and yield protection in agricultural production. The anti-transpiration agents are classified into three types according to different action characteristics of the anti-transpiration agents, namely, film type anti-transpiration agents, reflection type anti-transpiration agents and metabolism type anti-transpiration agents. The film type anti-transpiration agent has the active ingredient of an organic high molecular compound, and forms a layer of soft and smooth film on the leaf surface by spraying, so that the water which is diffused into the air through the air holes is greatly reduced; the reflective anti-transpiration agent is sprayed on the upper surfaces of the plant leaves, so that part of solar radiation energy can be reflected, and the solar radiation absorbed by the leaves is reduced, thereby reducing the temperature of the leaves and transpiration; the metabolic anti-transpiration agent can close the air holes or reduce the opening of the air holes, thereby increasing the transpiration resistance of the air holes and inhibiting the transpiration.
The conventional film type anti-transpiration agent not only can increase the water potential of plant leaves and fruits, but also can improve the crop yield, improve the plant transplanting and cuttage survival rate, has good effects of warming and preserving moisture, resisting insect diseases, improving plant resistance and the like, is more effective than other two types of anti-transpiration agents under the condition of short-term drought, and is widely applied to the fields of ornamental greening tree species, big tree transplanting, fruit, vegetable, flower fresh keeping and the like. At present, the active ingredients of the film type anti-transpiration agent mainly comprise silicone, polyethylene, polyvinyl chloride, paraffin, rosin diene (terpene resin) and the like. Wherein, the pinolene is a beta-pinene polymer catalyzed by Lewis acid with low molecular weight, is one of main components of pine oil, is light yellow viscous liquid at normal temperature, and has aromatic smell. Pinolene is an organic polymer composed entirely of carbon and hydrogen, and is relatively non-toxic to mammals, LD 50 Exceeding 20000. It has excellent film forming performance, and after spraying, it forms transparent and soft film to reduce water loss of plant obviously without interfering with plant growth or normal respiration. Because the pinolene has the characteristics of excellent film forming property, high safety and the like, the application of the foreign film type anti-transpiration agent is wider, such as Wilt Pruf and Vapor Gard.
Although the effect of the conventional turpentine diene film type anti-transpiration agent is superior, the conventional turpentine diene film type anti-transpiration agent also has the following defects:
(1) The pinolene is a low molecular polymer, has large surface tension, poor wetting and spreading ability when being sprayed on plant leaves, is easy to fall off as water droplets, and has low utilization rate.
(2) The effect of anti-transpiration is limited, and the transpiration inhibition is carried out only by a physical mode of film formation.
The disease and insect resistant action mode is single, and the action effect on the plants infected with germs is poor. .
Disclosure of Invention
The invention aims to overcome the defects in the prior art, provides an anti-transpiration agent containing S-induced antibiotics and a preparation method thereof, and aims to solve the problems of poor wetting and spreading, limited anti-transpiration effect and single anti-insect action mode of the existing anti-film anti-transpiration agent.
In order to achieve the above object, the present invention adopts the following technical scheme:
the invention provides an anti-transpiration agent containing S-induced antibiotics, which comprises the following components in percentage by mass, based on 100% of the total mass of the anti-transpiration agent:
1-20% of S-induced antibiotics, 1-80% of pinolene and the balance of excipients.
The invention provides an anti-transpiration agent containing S-abscisic acid, which is applied to the fields of ornamental greening tree species, big tree transplanting, fruit, vegetable, flower fresh-keeping and the like, plays a role in inhibiting bacteria by activating an immune system and a defense mechanism of plants, promotes callose formation in an infected area, protects crop tissues from bacteria and infection, can promote crops from being affected by bacteria and fungus diseases under the condition of generating resistance, can regulate the closing of air holes of crop leaves, prevent the invasion of bacteria, block the invasion path of bacteria, reduce the incidence rate, and simultaneously slow down the transpiration, and has the effect of promoting the absorption of crops to nutrient elements such as potassium, calcium, magnesium, phosphorus and the like; the pinolene has excellent film forming performance, forms a transparent and soft film after spraying, can obviously reduce the water loss of plants, does not interfere the growth or normal respiration of the plants, resists the invasion of bacteria and pests, and forms a synergistic effect with S-abscisic acid with different action modes; meanwhile, the excipient has the function of endowing the anti-transpiration agent with a specific stable form, is convenient for circulation and use, and is suitable for the dispersing and wetting requirements of various application technologies. In a word, the anti-transpiration agent containing the S-induced antibiotic ensures that the S-induced antibiotic and the pinolene stably play a role through the synergistic effect of each component and content, does not use aromatic hydrocarbon solvents, is safe to produce and use, and is environment-friendly.
Further, the excipient is a solvent and/or a surfactant.
Preferably, the solvent is a mixture of more than two of water, ethanol, isopropanol, n-butanol, vegetable oil, rosin-based vegetable oil and mineral oil.
Preferably, the vegetable oil may be one or more than two of soybean oil, corn oil, cottonseed oil, coconut oil, rapeseed oil, peanut oil, sesame oil, or methyl esterified vegetable oil.
Preferably, the surfactant is at least one of nonionic surfactant, anionic surfactant, cationic surfactant and amphoteric surfactant.
Preferably, the nonionic surfactant may be one or more than two of fatty alcohol polyoxyethylene ether, fatty acid polyoxyethylene ether, fatty amine polyoxyethylene ether, polyarylphenol polyoxyethylene ether, alkoxy polyoxyethylene ether, alkanolamide polyoxyethylene ether, fatty acid ester polyoxyethylene ether, castor oil polyoxyethylene ether and trisiloxane polyoxyethylene ether.
Preferably, the anionic surfactant may be one or more of alkyl ether carboxylate, alkylbenzenesulfonate, alkyl sulfonate, alpha-olefin sulfonate, succinate sulfonate, polyoxyethylene ether sulfate, fatty amide taurate, fatty alcohol sulfate, phosphate, or a combination thereof, but is not limited thereto.
Preferably, the cationic surfactant may be one or more of ammonium salts, quaternary ammonium salts, amine salts, heterocyclic ring type, polymeric type, and phosphonium salt type.
Preferably, the amphoteric surfactant may be one or more of betaine type, imidazoline type, amino acid type, amine oxide type, or the like, but is not limited thereto.
On the other hand, the embodiment of the invention also provides a preparation method of the anti-transpiration agent containing the S-induced antibiotics, which comprises the following steps: and fully stirring and homogenizing the S-abscisic acid, the pinolene and the excipient to obtain the anti-transpiration agent containing the S-abscisic acid.
The preparation method of the anti-transpiration agent containing the S-induced antibiotics, provided by the embodiment of the invention, has simple production process and equipment and low cost.
Further, the anti-transpiration agent containing the S-abscisic acid provided by the embodiment of the invention is in the form of aqueous emulsion, emulsifiable concentrate or microemulsion.
Compared with the prior art, the invention has the following beneficial effects:
1. the S-abscisic acid has the function of regulating the closure of the air holes of the crop leaves, can slow down the transpiration, and has a synergistic effect with the pinolene with different action modes, thereby improving the transpiration resistance;
2. the S-elicitor has the functions of bacteriostasis, sterilization and pathogen invasion prevention, promotes the formation of callose in an infected area, protects crop tissues from pathogen and bacteria infection, forms a synergistic effect with the pinolene with different action modes, and improves the protection effect on plants;
3. the anti-transpiration agent can be in the form of aqueous emulsion, emulsifiable concentrate or microemulsion, the particle size of oil drops of the microemulsion diluent is nano-scale, the dispersion degree of active ingredients is high, the particle size is small, the wetting and spreading are excellent, the film forming property is good, the plant leaves which are difficult to wet are well wetted and spread, and meanwhile, the anti-transpiration agent is used on fruits, vegetables and flowers, and residual stains are not formed, so that the quality is affected;
4. the S-abscisic acid has the function of promoting the absorption of nutrient elements such as potassium, calcium, magnesium, phosphorus and the like by crops, and can improve the quality of plants and fruits.
Detailed Description
In order to make the technical problems, technical schemes and beneficial effects to be solved more clear, the invention is further described below with reference to the embodiments, but the scope of the invention is not limited to the scope expressed by the embodiments.
Formulation example 1% S-decoin-pinolene aqueous emulsion
Weighing 20% of the induction antibiotics, 1% of the pinolene, 20% of ethanol, 2% of soybean oil, 5% of fatty alcohol-polyoxyethylene ether and 100% of water, and fully stirring and homogenizing to obtain 21% of the S-induction antibiotics and pinolene aqueous emulsion.
Formulation example 2% 16% S-decoin pinolene emulsifiable concentrate
Weighing 15% of S-induced antibiotic, 1% of pinolene, 10% of isopropanol, 7% of castor oil polyoxyethylene ether and 100% of soybean oil, and fully stirring and homogenizing to obtain 16% of S-induced antibiotic and pinolene emulsifiable concentrate.
Formulation example 3 22% S-decoin-pinolene microemulsion
Weighing 20% of the induction antibiotic, 2% of the pinolene, 16% of ethanol, 6% of rapeseed oil, 6% of polyarylphenol polyoxyethylene ether, 3% of alkylbenzene sulfonate and 100% of water, and fully stirring and homogenizing to obtain the 22% of the induction antibiotic and pinolene aqueous emulsion.
Formulation example 4 24% S-decoin-pinolene aqueous emulsion
Weighing 20% of S-induced antibiotic, 4% of pinolene, 12% of n-butanol, 4% of mineral oil, 4% of succinate sulfonate, 3% of alkoxy polyoxyethylene ether and 100% of water, and fully stirring and homogenizing to obtain 24% of S-induced antibiotic and pinolene aqueous emulsion.
Formulation example 5% 20% S-decoin-pinolene microemulsion
Weighing 15% of S-induced antibiotic, 5% of pinolene, 10% of ethanol, 5% of peanut oil, 5% of fatty acid polyoxyethylene ether, 4% of alkyl ether carboxylate and 100% of water, and fully stirring and homogenizing to obtain 20% of S-induced antibiotic and pinolene microemulsion.
Formulation example 6% S-decoin-pinolene emulsifiable concentrate
Weighing 20% of S-induced antibiotic, 10% of pinolene, 12% of isopropanol, 5% of fatty acid ester polyoxyethylene ether, 4% of polyoxyethylene ether sulfate and 100% of turpentine vegetable oil, and fully stirring and homogenizing to obtain 30% of S-induced antibiotic and turpentine diene emulsifiable concentrate.
Formulation example 7 40% S-decoin-pinolene aqueous emulsion
Weighing 20% of S-induced antibiotic, 20% of pinolene, 10% of n-butanol, 13% of methyl oleate, 4% of alkanolamide polyoxyethylene ether, 3% of alpha-olefin sulfonate and 100% of water, and fully stirring and homogenizing to obtain 40% of S-induced antibiotic/pinolene aqueous emulsion.
Formulation example 8 45% S-decoin-pinolene aqueous emulsion
Weighing 15% of the induction agent, 30% of the pinolene, 8% of n-butanol, 12% of peanut oil, 5% of fatty acid ester polyoxyethylene ether, 5% of fatty amide taurine salt and 100% of water, and fully stirring and homogenizing to obtain 45% of the S-induction agent-pinolene aqueous emulsion.
Formulation example 9 20% S-decoin-pinolene aqueous emulsion
Weighing 5%S-induced antibiotic, 15% pinolene, 5% ethanol, 12% peanut oil, 3% fatty alcohol polyoxyethylene ether, 4% benzalkonium chloride and 100% water, and fully stirring and homogenizing to obtain 20% S-induced antibiotic-pinolene aqueous emulsion.
Formulation example 10% S-decoin-pinolene aqueous emulsion
Weighing 10% of S-induced antibiotic, 50% of pinolene, 10% of ethanol, 10% of coconut oil, 5% of cocamidopropyl betaine, 3% of fatty alcohol polyoxyethylene ether phosphate and 100% of water, and fully stirring and homogenizing to obtain 60% of S-induced antibiotic and pinolene aqueous emulsion.
Formulation example 11 44% S-decoin-pinolene aqueous emulsion
Weighing 4% of S-induced antibiotic, 40% of pinolene, 5% of isopropanol, 14% of soybean oil, 8% of cocamidopropyl amine oxide, 3% of fatty alcohol polyoxyethylene ether phosphate and 100% of water, and fully stirring and homogenizing to obtain 44% of S-induced antibiotic and pinolene aqueous emulsion.
Formulation example 12 32% S-decoin pinolene emulsifiable concentrate
Weighing 2%S-induced antibiotic, 30% pinolene, sesame oil 12%, trisiloxane polyoxyethylene ether 4%, alkyl sulfonate 5% and peanut oil to complement 100%, and fully stirring and homogenizing to obtain 32% S-induced antibiotic and pinolene emulsifiable concentrate.
Formulation example 13% S-decoin-pinolene aqueous emulsion
Weighing 2%S-induced antibiotic, 40% pinolene, 16% rapeseed oil, 5% castor oil polyoxyethylene ether, 5% polyarylphenol polyoxyethylene ether and 100% water, and fully stirring and homogenizing to obtain 42% S-induced antibiotic-pinolene water emulsion.
Biological assay example 1 test of effect of anti-transpiration agent containing S-abscisic acid on reduction of wheat transpiration
Test crop: wheat in tillering stage
The test method comprises the following steps: most of the water absorbed by the plants is dissipated to the outside through the transpiration, only a small part of the water is supplied to the life activities, and when the transpiration is restrained, the water absorbed by the plants is correspondingly reduced, so that the transpiration strength can be judged through the change of the water consumption of the plants.
Each test group selects 50 wheat strains with completely consistent physiological states, and irrigates the same amount of tap water for standby. Uniformly spraying an equal volume of water diluted anti-transpiration agent on the surfaces of the wheat leaves of the treatment group, uniformly spraying an equal volume of water on the surfaces of the wheat leaves of the blank group, investigating the residual water quantity once after 7 days of the first spraying, selecting fine weather, measuring the transpiration rate of the wheat leaves of each treatment group and control group by using an LI1600 steady-state porosimeter at 12:00 noon, and measuring the residual water quantity and the transpiration rate of the leaves again after 7 days of the second spraying after 10 days. The calculation formulas of the amount of the anti-transpiration agent and the water saving rate of each test group are shown in the following table 1.
Test group: the antitranspirant prepared in each example was sprayed
Control group: spraying with the same volume of common concentration of commercially available antitranspirant Wilt Pruf
Blank group: spraying water with the same volume as that of experimental group
Water saving% = (blank water consumption-treatment water consumption)/blank water consumption 100 ×
TABLE 1 test of effect of S-abscisic acid-containing antitranspirant on reducing wheat transpiration
As can be seen from table 1, the water consumption and the transpiration rate of the anti-transpirant treatment group in the examples of the present invention were significantly lower than those of the pinolene, S-abscisic acid single agent group and blank group, and the water consumption and the transpiration rate of the anti-transpirant treatment groups 6, 7, 8 and 9 in the examples were significantly lower than those of the anti-transpirant Wilt Pruf in the control group. The water saving rate of each example of the treatment group is obviously higher than that of a pinolene, S-induced antibiotic single agent group and a blank group, and the water saving rate of the anti-transpiration agent treatment groups of 6, 7, 8 and 9 in the examples is obviously higher than that of the anti-transpiration agent Wilt Pruf of the control group. The result shows that the combined treatment of the pinoresinol diene and the S-induced resistance element has obvious water-saving effect on wheat. In the test process, the physiological state of each treated wheat is normal, and the morphology is not abnormal.
Biological assay example 2 Effect test of anti-transpiration agent containing S-abscisic acid for reducing the transpiration of jujube tree
Test crop: jujube tree
The test method comprises the following steps: after the seedlings are lifted, the anti-transpiration agents of each treatment are sprayed on the jujube seedlings, and the sprayed clear water is used as a control, and then
And then carrying out airing treatment, wherein the airing time is respectively set to be 24 hours and 48 hours. Each treatment randomly picked 50 seedlings of consistent growth vigor. The average temperature of the sun during the airing period is 18 ℃, the average ground temperature of the sun is 16 ℃, and the average relative humidity is 40%. And (5) investigation and recording the water loss rate and survival rate of each treated seedling.
Water loss rate of nursery stock: randomly extracting 5 plants from 50 plants to be treated, uniformly spraying an anti-transpiration agent on the whole plant according to a test design, cutting off the plant from the root and stem, and equally dividing and weighing the fresh weight of the root system and the fresh weight of the stem of the seedling by an electronic day. And (3) placing the seedling stems and root systems weighed by the fresh weights outdoors, performing airing treatment according to a test design, weighing again after the designed treatment time, and calculating the water loss rate of the seedlings according to the fresh weights before airing and the weights after airing. The test results are shown in Table 2.
Water loss = (fresh weight before drying-fresh weight after drying)/fresh weight before drying x 100
Survival rate of seedlings: planting the treated seedlings according to a normal method, taking the sprouted and spread seedlings as a survival standard, investigating the survival number of the seedlings which are treated differently in the whole test area after 60 days, and calculating the survival rate of each treatment.
Table 2 test of the effect of reducing the transpiration of jujube trees with an S-abscisic acid anti-transpiration agent
As can be seen from the results in Table 2, the water loss rate of the anti-transpiration agent jujube seedlings in the compound treatment example of the invention by spraying the pinolene and the S-induced antibiotics is obviously lower than that of the pinolene, the S-induced antibiotics single agent group and the blank group, and the survival rate of the anti-transpiration agent jujube seedlings in the example is obviously higher than that of the pinolene, the S-induced antibiotics single agent group and the blank group, which indicates that the water saving effect of the anti-transpiration agent in each compound treatment group is obvious. The anti-transpiration agent containing S-abscisic acid plays a positive role in inhibiting water loss of seedlings, improving water content of the seedlings and roots, prolonging survival time of the seedlings and is beneficial to seedling reviving and transplanting. In the test process, the physiological state of each treatment jujube tree is normal, and the morphology is not abnormal.
Bioassay example 3 field efficacy test of S-induced antibiotic on wheat root rot
Test crops and diseases: root rot of wheat, wheat in seedling stage
The test method comprises the following steps: each processing cell adopts a random arrangement mode, each processing is repeated for 4 times, and the cell area is 12.5m 2 And a protection row is arranged in each cell. Root irrigation is carried out once every 15 days for each treatment, medicine irrigation is carried out continuously for 2 times, random five-point sampling is adopted in each cell, 1m double-line investigation is adopted at fixed points for each treatment of the disease condition of wheat, investigation is carried out by taking plants as units, total plant number and wheat disease plant number are recorded and investigated, the disease rate and prevention effect are calculated, and the calculation formulas of the disease rate and the prevention effect are as follows. Each district adopts random five-point sampling, each point takes 0.5 meter double-line investigation of the plant height of agronomic character indexes of each processed wheat, the root length and the whole fresh weight of yield indexes, and the plant height, the root length and the fresh weight difference of each processed wheat are compared. The test results are shown in Table 3.
Incidence% = number of diseased plants/total number of investigated plants 100
Control effect% = (control area disease rate-treatment area disease rate)/control area disease rate 100%.
Table 3S field efficacy test of the elicitor on wheat root rot
As can be seen from the results in Table 3, the incidence rate of wheat root rot of the compound treatment group of S-induced antibiotic and pinoresinol diene in the embodiment of the invention is obviously lower than that of the compound treatment group of pinoresinol diene, S-induced antibiotic single agent and blank group, and the prevention effect of each treatment in the compound embodiment is obviously higher than that of the compound treatment group of pinoresinol diene and S-induced antibiotic single agent, which indicates that the compound treatment group of S-induced antibiotic in the embodiment has good prevention effect on wheat root rot. The results of the wheat agronomic character and yield index measurement show that the results of the plant height, root length and plant fresh weight of the wheat of the S-lure antibiotic and pinoresinol diene compound treatment group are obviously higher than those of the pinoresinol diene, the S-lure antibiotic single agent group and the blank group, and the S-lure antibiotic and pinoresinol compound treatment group in the embodiment has obvious promotion effect on the wheat growth. In the test process, the physiological state of each treated wheat is normal, and the morphology is not abnormal.
From the test results of the application examples, the S-abscisic acid has excellent anti-transpiration and water-saving effects on wheat and jujube trees, and is beneficial to improving the survival rate of jujube tree seedlings. The S-elicitor has excellent prevention effect on wheat root rot and good growth promoting effect on wheat seedlings. Meanwhile, the S-abscisic acid is found to be safe to wheat and jujube trees in the test process, and is an anti-transpiration agent worthy of popularization and application.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the invention.

Claims (3)

1. An antitranspirant comprising S-abscisic acid, characterized in that: the anti-transpiration agent consists of the following components in percentage by mass based on 100% of the total mass of the anti-transpiration agent: 1-20% of S-induced antibiotics, 1-80% of pinolene and the balance of excipients; the excipient is a solvent and/or a surfactant; the solvent is a mixture of more than two of water, ethanol, isopropanol, n-butanol, vegetable oil, rosin-based vegetable oil and mineral oil; the surfactant is at least one of nonionic surfactant, anionic surfactant, cationic surfactant and amphoteric surfactant; the vegetable oil is one or more than two of soybean oil, corn oil, cotton seed oil, coconut oil, rapeseed oil, peanut oil, sesame oil or methyl esterification vegetable oil; the nonionic surfactant is one or more than two of fatty alcohol polyoxyethylene ether, fatty acid polyoxyethylene ether, fatty amine polyoxyethylene ether, polyarylphenol polyoxyethylene ether, alkoxy polyoxyethylene ether, alkanolamide polyoxyethylene ether, fatty acid ester polyoxyethylene ether, castor oil polyoxyethylene ether and trisiloxane polyoxyethylene ether; the anionic surfactant is one or more than two of alkyl ether carboxylate, alkylbenzene sulfonate, alkyl sulfonate, alpha-olefin sulfonate, succinic acid ester sulfonate, polyoxyethylene ether sulfate, fatty amide taurine, fatty alcohol sulfate and phosphate; the cationic surfactant is one or more than two of ammonium salt, quaternary ammonium salt, amine salt, heterocyclic ring type, polymeric type and onium salt type; the amphoteric surfactant is one or a mixture of more than two of betaine type, imidazoline type, amino acid type and amine oxide type.
2. A method for producing an antitranspirant comprising S-abscisic acid according to claim 1, characterized in that: and fully stirring and homogenizing the S-abscisic acid, the pinolene and the excipient to obtain the anti-transpiration agent containing the S-abscisic acid.
3. An anti-transpiration agent comprising S-abscisic acid as set forth in claim 1, wherein: the antitranspirant containing S-abscisic acid is in the form of aqueous emulsion, emulsifiable concentrate or microemulsion.
CN202110122537.5A 2021-01-29 2021-01-29 Anti-transpiration agent containing S-abscisic acid and preparation method thereof Active CN114794112B (en)

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GB2016436A (en) * 1978-02-11 1979-09-26 Basf Ag Reduction in the transpiration of crop plants
CN106719611A (en) * 2016-12-30 2017-05-31 胡琳 A kind of flower antistaling agent and preparation method thereof
WO2017166564A1 (en) * 2016-04-02 2017-10-05 江苏辉丰农化股份有限公司 Plant growth regulator composition
CN111117412A (en) * 2019-12-19 2020-05-08 周雯 Film type plant antitranspirant
CN112075455A (en) * 2020-09-22 2020-12-15 广东筑兴建设工程有限公司 Efficient antitranspirant and application method thereof in full-crown transplanting of big trees

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2016436A (en) * 1978-02-11 1979-09-26 Basf Ag Reduction in the transpiration of crop plants
WO2017166564A1 (en) * 2016-04-02 2017-10-05 江苏辉丰农化股份有限公司 Plant growth regulator composition
CN106719611A (en) * 2016-12-30 2017-05-31 胡琳 A kind of flower antistaling agent and preparation method thereof
CN111117412A (en) * 2019-12-19 2020-05-08 周雯 Film type plant antitranspirant
CN112075455A (en) * 2020-09-22 2020-12-15 广东筑兴建设工程有限公司 Efficient antitranspirant and application method thereof in full-crown transplanting of big trees

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