CN114847404A - Composition for reducing stress response of deep and open sea cultured fishes and living body transfer method - Google Patents
Composition for reducing stress response of deep and open sea cultured fishes and living body transfer method Download PDFInfo
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Images
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention belongs to the technical field of deep and open sea culture, and discloses a composition for reducing stress response of deep and open sea cultured fishes and a living body transfer method. Before the fish is caught and transported, the composition containing succinic acid, oyster shell powder, polygala tenuifolia micro powder and spina date seed micro powder is added into feed and continuously fed for 5-6 days. Before transportation, vitamin C and vitamin E are respectively added into the aquaculture water body to soak and transport fishes. And then, transferring the fishes to a live fish ship transfer pond, adding a proper amount of polyhexamethylene guanidine disinfectant, transferring the fishes to a culture net cage or a culture pond by using a fish sucking pump, stopping feeding for 1-2 days, adding beta carotene into the feed after the fishes recover the activity, and continuously feeding for 4-5 days to recover normal culture. The invention establishes a living body transfer method capable of effectively reducing the stress response of deep-open sea cultured fishes, and provides an important technical support for the healthy development of the deep-open sea fish culture industry.
Description
Technical Field
The invention belongs to the technical field of deep and open sea aquaculture, and particularly relates to a composition for reducing stress response of deep and open sea aquaculture fishes and a living body transfer method for reducing stress response of deep and open sea aquaculture fishes.
Background
Deep open sea culture is a strategic direction for the development of the mariculture industry in China and is also one of the main industrial modes for the development of marine economy in China. At the present stage, the main variety of deep and open sea culture is economic fish, the culture mode mainly comprises large net cages and culture ships, and the deep and open sea culture has the outstanding advantages of high culture density, high benefit, small environmental hazard, good product quality and the like. Due to the special environment and culture mode of deep and open sea culture, the fish fries put in the deep and open sea culture are large-scale fries with the weight of 100 plus 400g, and even fries with the weight of more than 1000g need to be put in individual fish varieties. The seedlings are mainly from land-based industrial aquaculture plants or offshore net cages and must be transported to large net cages or aquaculture ships in deep and open sea for relay aquaculture by means of live body transportation. The large net cages and the aquaculture ships in deep and open sea are generally far away from the seashore line, and the fry living body transportation process involves a plurality of operation processes such as catching, shipping, transporting and transferring, so that the fry is very easy to cause physiological stress and mechanical damage, the immunity of the organism is reduced, pathogenic infection and diseases are further caused, and the aquaculture survival rate is finally influenced.
From the actual culture production experience, the phenomenon that fry death in a large amount in a short time after entering a large-scale net cage in deep open sea and a culture ship is caused by improper operation in the transfer process is common, so that serious economic loss is caused for culture enterprises, and the production enthusiasm of the culture enterprises is also struck to a certain extent. Therefore, the establishment of the living body transfer method capable of effectively reducing the stress response of the cultured fishes in the deep open sea is an important technical requirement for ensuring the health and stable development of the deep open sea industry.
Through the above analysis, the problems and defects of the prior art are as follows:
(1) in the prior art, due to the lack of a safe and effective transfer technical method, the cultured fishes are easy to have stress reaction and mechanical damage in the transfer process of the terrestrial sea relay, so that the infection and the disease of pathogens are easy to cause, the large-scale death of the transferred fishes is caused, and the serious economic loss is caused to the culture enterprises.
(2) In the prior art, a certain mechanical operation means is required for catching and transporting fishes, so that the cultured fishes are bound to generate physiological stress reaction, the immunity is reduced, and body surface injury is formed.
The difficulty in solving the above problems and defects is:
(1) the living body transfer of the large-size fry is an essential production link in the deep open sea culture process. Generally, live fry transferred through the link are large in number and high in density, and have many mechanical operations such as fishing, transferring and transporting, so that the transferred fry mostly have various phenomena such as strong stress response, reduced immunity, mechanical damage on the body surface and the like, and the final transfer survival rate is directly influenced. At present, in actual production operation, no special preventive treatment measures are provided for the process, most of the measures depend on the production experience of culturists, no standardized operation process and method exist, and the technical support for cultivation production is difficult to form. Therefore, a technical method for safely transferring live fishes cultured in deep sea, land and sea in relay mode is urgently needed to be developed, and the survival rate of the transfer is guaranteed.
(2) According to the basic theory and practical experience of medicine and veterinary medicine, when an organism has a stress reaction, the damage of the stress reaction to the organism can be reduced and the immunity of the organism can be enhanced by eating special medicines or nutrients. However, for deep and open sea aquaculture production, what substances are used and how to use the substances efficiently can effectively reduce the body stress damage of the farmed fish in the living body transportation process, enhance the immune response and reduce the risk of pathogenic infection, so that the method is blank at present, needs to be researched and developed initiatively, and has certain technical difficulty.
The significance of solving the problems and the defects is as follows: deep open sea aquaculture is a new industry of marine economy in China and is also a strategic direction of development of the marine aquaculture industry in China. However, the key element of the healthy development of the industry is that a perfect process technology system is provided to ensure higher culture survival rate, thereby generating remarkable economic benefit. According to the current production experience, the method is suitable for the safe transfer of the living bodies of the cultured fish fries, and has decisive influence on the later culture survival rate. Therefore, the technical method for reducing the stress response and the mechanical injury in the live fry transferring process is established, and has important practical significance for the safe production of deep open sea aquaculture.
Disclosure of Invention
In order to overcome the problems in the related art, the disclosed embodiment of the invention provides a composition for reducing the stress response of cultured fishes in deep open sea and a live body transferring method of the cultured fishes, and particularly relates to a live body transferring method for reducing the stress response of the cultured fishes in deep open sea.
The technical scheme is as follows: a composition for reducing stress response of deep sea cultured fishes comprises, by mass, 10-15% of succinic acid, 25-35% of oyster shell micro powder, 25-35% of polygala tenuifolia micro powder and 15-35% of spina date seed micro powder.
In one embodiment, the composition for reducing the stress response of the deep sea farmed fish comprises, by mass, 10% of succinic acid, 30% of oyster shell micro powder, 30% of polygala tenuifolia micro powder and 30% of spina date seed micro powder.
In one embodiment, the particle size of the oyster shell micro powder is more than or equal to 100 meshes, the particle size of the polygala tenuifolia micro powder is more than or equal to 100 meshes, and the particle size of the spina date seed micro powder is more than or equal to 100 meshes.
Another object of the present invention is to provide a method for reducing stress response of deep-open-sea farmed fish using the composition for reducing stress response of deep-open-sea farmed fish, the method comprising: before the fishes are caught and transported, the composition for reducing the stress response of the deep open sea cultured fishes is added into feed to feed the fishes to be transported.
In one embodiment, the composition for reducing the stress response of the deep and open sea cultured fishes is added into fish feed according to the dosage of 15-20 g/kg 5-6 days before the fishes are caught and transported;
and stopping feeding the feed 1 day before the fish are caught and transported.
In one embodiment, the composition for reducing the stress response of the deep-open sea farmed fish is added to the feed to feed the fish to be transported, and then the following steps are carried out:
before transportation, fish to be transported are concentrated, and vitamin C and vitamin E are added into water for soaking; then, transferring the fish to a water tank of a live fish boat by using a fish sucking pump, controlling the water temperature of the water tank according to different fish varieties, adding a proper amount of polyhexamethylene guanidine hydrochloride aqueous solution into the water in the transferring tank, and starting transferring by using the live fish boat;
after the live fish boat is close to the culture facility, the fish is conveyed into the culture facility by using a fish sucking pump, the feeding is stopped for 1-2 days, after the vitality of the transported fish is completely restored, the beta carotene is added into the feed, and the normal culture is restored after the fish is continuously fed for several days.
In one embodiment, the fish are harvested 1 hour before transportation at 15g/m 3 The vitamin C and the vitamin E are respectively added into the pond water, the fish body to be transported is soaked, and the soaking process is aerated.
In one embodiment, the fish soaked in vitamin C and vitamin E for 1 hour is transferred to the water in the live fish boat by a fish pump, and the temperature of the water is controlled to be 5 to EThe biomass density in the pool is less than or equal to 40kg/m at 15 DEG C 3 And in an amount of 0.1 to 0.3ml/m 3 The amount of the compound is that a 25 percent solution of polyhexamethylene guanidine hydrochloride is added into the pool water.
In one embodiment, the fish seeds are transferred from the live fish boat to the net cage or the culture pond of the industrial boat by using the fish sucking pump, and the fish seeds are stopped from feeding for 1-2 days.
In one embodiment, after the fry recover normal activity, the feed is added with beta carotene according to the dosage of 10-15 g/kg.
In one embodiment, the feed added with beta carotene is fed once in the morning, in the middle and at night every day, and the normal culture is recovered after continuous feeding for 4-5 days.
By combining all the technical schemes, the invention has the advantages and positive effects that:
the living body transferring method capable of effectively reducing the stress response of the cultured fishes is established, a solution is provided for safe transferring of deep and open sea adaptive fish terrestris relay, the risk of diseases of fish fries can be obviously reduced, the survival rate of the fish fries after transferring is effectively guaranteed, and important technical support is provided for guaranteeing the healthy and stable development of the deep and open sea culture industry.
The technical scheme established by the invention is an important technical basis for guaranteeing the survival rate of the cultured fishes in the deep open sea and is also an important supporting technology for creating remarkable economic benefits in the deep open sea culture industry. By taking a large-scale net cage with the capacity of 5 million cubic meters as an example, more than 60 million fish of sebastes schlegeli hilgendorf can be cultivated at one time. The traditional transfer mode is that the fish is directly caught and transferred to a live fish ship from an offshore small net cage by using a hanging basket, the fish is transferred to a large net cage by using the hanging basket again after being transferred to the large net cage by using the live fish ship, the live fish is subjected to twice water separation process, and meanwhile, as Sebastes schlegeli has very hard thorns, the mutual puncture is very serious, pathogenic infection is easily caused after the wound is formed, further diseases are caused, and large-scale death is caused. In the actual production, the large-scale death of fishes after entering the net cage due to improper transfer occurs for many times, the highest death rate of the single-port net cage reaches more than 70%, and the economic loss exceeds 1000 ten thousand yuan. Therefore, the technical scheme of the invention can effectively reduce the risk of large-scale death of the fishes after transportation, avoid economic loss and have huge commercial value.
The living body transferring method for reducing the stress response of the cultured fishes in the deep and open sea can be applied to living body transferring of the fishes among different culture facilities.
And thirdly, the deep and open sea aquaculture industry is just emerging in China, various technical processes are still in the exploration and research stage, and production practice verification is needed. The live fish transferring suitable for culturing the fish fries is an indispensable key production link for developing deep open sea culture, and the success or failure of live fish transferring directly determines the success or failure of subsequent culture. Before the invention, China has no mature technical scheme, and the field basically belongs to the blank. The technical scheme determined by the invention fills up the technical blank in the field of live fish transportation in deep and distant sea in China.
Fourthly, whether the technical scheme of the invention solves the technical problem which is always desired to be solved but is not successful is determined: how to ensure the safe transfer of the living bodies of the fish fries is one of the most concerned technologies of enterprises and practitioners engaged in deep open sea culture production, and the high-quality live fish transfer has great significance for the success of the final culture and the achievement of ideal economic benefits. However, for a long time, the live fish fries are transported by the traditional experience in the cultivation and production practice, so that the quality of the fish fries transported each time is uneven, and the phenomenon of mass death after transportation frequently occurs. The technical scheme provided by the invention provides a scientific solution for the efficient transfer of living bodies of deep-open sea cultured fishes, breaks through the technical problem of living body transfer of fish fries, meets the technical requirements of people in the field, perfects the technical system of deep-open sea healthy culture production, and has positive significance for ensuring the sustainable development of industry.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the disclosure.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present disclosure and together with the description, serve to explain the principles of the disclosure.
FIG. 1 is a flow chart of a method for reducing stress response of cultured fish in deep open sea according to an embodiment of the present invention.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, but rather should be construed as broadly as the present invention is capable of modification in various respects, all without departing from the spirit and scope of the present invention.
First, in order to make those skilled in the art fully understand how to implement the present invention, the section is an explanatory embodiment for explaining the technical solution of the claims.
The embodiment of the invention provides a composition for reducing stress response of deep sea fish culture, which comprises, by mass, 10-15% of succinic acid, 25-35% of oyster shell micro powder, 25-35% of polygala root micro powder and 15-35% of spina date seed micro powder.
In a preferred embodiment of the invention, the succinic acid is 10%, the oyster shell micro powder with the granularity of more than 100 meshes is 30%, the polygala tenuifolia micro powder with the granularity of more than 100 meshes is 30%, and the spina date seed micro powder with the granularity of more than 100 meshes is 30%. The above are all mass percentages.
Before the fish is caught and transported, the composition containing succinic acid, oyster shell powder, polygala tenuifolia micro powder and spina date seed micro powder is added into feed according to the dosage of 15-20 g/kg, and the feed is continuously fed for 5-6 days. Stopping feeding in the first 1 day of formal transportation, and emptying the digestive tract of the fish to be transported. 1 hour before transportation, according to 15g/m 3 The vitamin C and the vitamin E are respectively added into the aquaculture water body to soak and transport the fishes. Then, the fishes are transferred into a live fish ship transfer pond, the reasonable density is controlled according to different fish species, the lower water temperature is kept, and meanwhile, a proper amount of polyhexamethylene oxide is added into the pond waterThe methyl guanidine disinfectant can prevent fish from mechanical damage and infection. After the live fish boat is close to the large-scale culture facility, the fish is transferred to a culture net cage or a culture pond by using a fish sucking pump, the fish is stopped from eating for 1-2 days, after the activity of the fish is recovered, beta carotene is added into the feed, and the normal culture can be recovered after the fish is continuously fed for 4-5 days. The invention establishes a living body transfer method capable of effectively reducing the stress response of deep-open sea cultured fishes, and provides an important technical support for the healthy development of the deep-open sea fish culture industry.
The technical solution of the present invention is further described below with reference to the specific embodiments and the accompanying drawings.
Example 1
As shown in FIG. 1, the present invention provides a method for reducing stress response of cultured fish in deep open sea, comprising:
s101, adding the composition for reducing the stress response of the cultured fishes in the deep and open sea into fish feed according to the dosage of 15-20 g/kg 5-6 days before the fishes are caught and transported. Feeding the fish fries to be transported twice a day in the morning and at night, wherein the feeding time is about 8 am and about 7 pm, and the feeding is continuously carried out for 4 days.
S102, stopping feeding the feed 1 day before the fishes are caught and transported, and emptying the digestive tracts of the fishes to be transported.
S103, 1 hour before the fishes are caught and transported, collecting the fishes to be transported in a small range by waterproof canvas according to the weight of 15g/m 3 The vitamin C and the vitamin E are respectively added for soaking, and the soaking process needs to be kept aerated, so that the dissolved oxygen in the water body is ensured to be more than or equal to 7mg/L, and the phenomenon of oxygen deficiency of the fishes is prevented.
S104, transferring the fish fries soaked for 1 hour by the vitamin C and the vitamin E into water of a live fish boat pool by using a fish sucking pump, and inflating the water of the pool to ensure that the dissolved oxygen of the pool water is not less than 7 mg/L. Controlling the temperature of the pool water of the live fish boat within the range of 5-15 ℃ (selecting proper water temperature according to different fish species, preferably slightly higher than the lowest survival water temperature of the fish), and simultaneously controlling the temperature to be 0.1-0.3 ml/m 3 The dosage of the method is that 25 percent of polyhexamethylene guanidine is added into pool waterAn aqueous hydrochloride salt solution. At the moment, the live fish boat can be opened to carry out live fish transfer to the large net cages or the aquaculture worker boats in the deep and open sea.
S105, after the live fish boat approaches to the deep open sea large net cage or the cultivation worker boat, timely transferring fish fries from the live fish boat to a cultivation facility by using a fish suction pump, stopping feeding for 1 day, adding beta carotene into feed according to the dosage of 10-15 g/kg, feeding once in the morning, in the middle and at night every day, continuously feeding for 4-5 days, and then starting normal cultivation.
Example 2
The embodiment of the invention provides a composition for reducing stress response of deep sea fish culture, which comprises, by mass, 10% of succinic acid, 25% of oyster shell micro powder, 25% of polygala root micro powder and 30% of spina date seed micro powder. The preparation method of the composition for reducing the stress response of the deep open sea cultured fishes is the same as that of the example 1.
Example 3
The embodiment of the invention provides a composition for reducing stress response of deep sea fish culture, which comprises, by mass, 15% of succinic acid, 25% of oyster shell micro powder, 25% of polygala root micro powder and 35% of spina date seed micro powder. The preparation method of the composition for reducing the stress response of the deep open sea cultured fishes is the same as that of the example 1.
Example 4
The embodiment of the invention provides a composition for reducing stress response of deep sea fish culture, which comprises 12.5 percent of succinic acid, 30 percent of oyster shell micro powder, 30 percent of polygala root micro powder and 27.5 percent of spina date seed micro powder in percentage by mass. The preparation method of the composition for reducing the stress response of the deep open sea cultured fishes is the same as that of the example 1.
Thirdly, in order to prove the creativity and the technical value of the technical scheme of the invention, the part is the application example of the technical scheme of the claims on specific products or related technologies.
In 2021, in 5 months, 30 million Sebastes schlegeli with average body weight of about 130g cultured in a certain land-based industrial farm in a certain city are transported to a large wind wave-resistant net cage in a certain sea area by using the technical method of the invention to carry out relay culture in land and sea, and the concrete implementation operations are as follows:
(1) starting from the first 6 days of the transportation of large-specification offspring seeds of sebastes schlegeli hilgendorf industrially cultured, a composition for reducing the stress response of fish cultured in deep open sea is added into fish feed according to the dosage of 20g/kg, and the composition mainly comprises 10% of succinic acid, 30% of oyster shell micro powder, 30% of polygala tenuifolia micro powder and 30% of spina date seed micro powder in percentage by mass. The particle diameters of the oyster shell micro powder, the polygala root micro powder and the spina date seed micro powder are all more than or equal to 100 meshes. The feed added with the composition is fed for 1 time respectively at 8 am and 6 pm every day for 5 days continuously.
(2) Stopping feeding the feed in the first 1 day of transferring sebastes schlegeli hilgendorf offspring seeds, and emptying the digestive tract of the fish offspring seeds to be transferred.
(3) 15g/m in the first 1 hour of transport 3 The vitamin C and the vitamin E are respectively added into the culture pond for soaking, and the soaking process is aerated to ensure that the dissolved oxygen in the water body is more than or equal to 8 mg/L. After soaking for 1 hour, transferring the live fish fries in the industrial culture pond to a live water truck, quickly transferring the live fish fries to a wharf by using a fish suction pump, transferring the live fish fries to a transfer pond of a live fish boat again, and ensuring that the biomass of the fries in the transfer pond of the live fish boat is less than or equal to 30kg/m 3 。
(4) Controlling the water temperature of the transfer pond of the live fish ship within the range of 5-10 ℃ according to 0.1ml/m 3 Adding 25% polyhexamethylene guanidine hydrochloride aqueous solution into the pond water, starting an inflator pump to inflate the pond water, so that the dissolved oxygen of the pond water is more than or equal to 8mg/L, and simultaneously starting a live fish boat for transportation.
(5) After a live fish boat reaches a culture net cage in the long island sea area, fish fries are transferred into the net cage from a live fish boat transfer pond by using a fish sucking pump, after the fish fries are stopped eating for 1 day, beta carotene is added into the feed according to the use amount of 15g/kg, the fish fries are respectively fed for 1 time at 8 am, 12 am and 6 pm every day, and after the fish fries are continuously fed for 4-5 days, the fish fries can be changed into common feed for normal culture.
By 11 months 2021, by using the technical method disclosed by the invention, sebastes schlegeli transferred to the wind wave-resistant net cage of the long island sea area from the Weihai land-based industrial farm has good vitality, normal ingestion and normal growth, no serious diseases occur, the survival rate of culture reaches more than 95%, and the method fully proves that the transferring of live fry by using the technical method disclosed by the invention has a better protection effect.
This application is intended to cover any variations, uses, or adaptations of the disclosure following, in general, the principles of the disclosure and including such departures from the present disclosure as come within known or customary practice within the art to which the disclosure pertains. It is intended that the specification and examples be considered as exemplary only, with a true scope and spirit of the disclosure being indicated by the following claims. It will be understood that the present disclosure is not limited to the precise arrangements described above and shown in the drawings and that various modifications and changes may be made without departing from the scope thereof.
Claims (10)
1. The composition for reducing the stress response of the deep-open sea cultured fishes is characterized by comprising, by mass, 10-15% of succinic acid, 25-35% of oyster shell micro powder, 25-35% of polygala root micro powder and 15-35% of spina date seed micro powder.
2. The composition for reducing the stress response of the deep sea farming fishes according to claim 1, wherein the particle size of the oyster shell micro powder is not less than 100 meshes, the particle size of the polygala tenuifolia micro powder is not less than 100 meshes, and the particle size of the spina date seed micro powder is not less than 100 meshes.
3. The composition for reducing the stress response of the deep-sea farmed fishes as claimed in claim 1, wherein the composition for reducing the stress response of the deep-sea farmed fishes comprises 10% of succinic acid, 30% of oyster shell micro powder, 30% of polygala root micro powder and 30% of spina date seed micro powder by mass percentage.
4. A method for reducing stress response of deep-open-sea farmed fish using the composition for reducing stress response of deep-open-sea farmed fish according to any one of claims 1 to 3, the method comprising: before the fishes are caught and transported, the composition for reducing the stress response of the deep open sea cultured fishes is added into feed to feed the fishes to be transported.
5. The in vivo transfer method for reducing stress response of deep and open sea farmed fish according to claim 4, characterized in that the composition for reducing stress response of deep and open sea farmed fish is added to the fish feed in an amount of 15-20 g/kg 5-6 days before the fish are caught and transported; stopping feeding the feed 1 day before the fish are caught and transported;
after the composition for reducing the stress response of the deep and open sea cultured fishes is added into feed and fed to the fishes to be transported, the following steps are carried out:
before transportation, fish to be transported are concentrated, and vitamin C and vitamin E are added into water for soaking; then, transferring the fish to a water tank of a live fish boat by using a fish sucking pump, controlling the water temperature of the water tank according to different fish varieties, adding a proper amount of polyhexamethylene guanidine hydrochloride aqueous solution into the water in the transferring tank, and starting transferring by using the live fish boat;
after the live fish boat is close to the culture facility, the fish is conveyed into the culture facility by using a fish sucking pump, the feeding is stopped for 1-2 days, after the vitality of the transported fish is completely restored, the beta carotene is added into the feed, and the normal culture is restored after the fish is continuously fed for several days.
6. The in vivo transfer method for reducing stress response of cultured fishes in deep open sea according to claim 5, wherein the stress response is 15g/m 1 hour before the fishes are caught and transported 3 The vitamin C and the vitamin E are respectively added into the pond water, the fish body to be transported is soaked, and the soaking process is aerated.
7. The living body transfer method for reducing stress response of deep and open sea farmed fish according to claim 5, characterized in that the fish soaked with vitamin C and vitamin E for 1 hour is transferred to the water of the live fish boat and pond by a fish suction pump, the temperature of the water in the pond is controlled to be 5-15 ℃, and the biomass density in the pond is less than or equal to 40kg/m 3 And in an amount of 0.1 to 0.3ml/m 3 The dosage of the method is that 25 percent of polyhexamethylene guanidine hydrochloride water is added into pool waterAnd (3) solution.
8. The in vivo transferring method for reducing the stress response of the cultured fishes in the deep open sea as claimed in claim 5, wherein the fish seeds are transferred from the live fish boat to the net cage or the culture pond of the industrial boat by a fish sucking pump and are stopped for 1-2 days.
9. The in-vivo transferring method for reducing stress response of deep and open sea farmed fish according to claim 5, characterized in that after normal activity of fry is recovered, beta carotene is added into feed according to the dosage of 10-15 g/kg.
10. The in vivo transfer method for reducing stress response of deep and open sea farmed fish according to claim 9, characterized in that the feed added with beta carotene is fed once in the morning, in the middle and at night every day, and the normal farming is resumed after 4-5 days of continuous feeding.
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| GB0912705D0 (en) * | 2009-07-22 | 2009-08-26 | Questor Group Ltd C | Open ocean fish farm |
| CN101810848A (en) * | 2009-11-09 | 2010-08-25 | 天津瑞贝特科技发展有限公司 | Traditional Chinese medicinal composite for reducing stress response of aquatic animals and preparation method and application thereof |
| CN111149761A (en) * | 2020-02-26 | 2020-05-15 | 中国水产科学研究院黄海水产研究所 | Method for reducing stress in transportation process of oplegnathus punctatus |
| CN113134049A (en) * | 2021-05-19 | 2021-07-20 | 湖南渔经生物技术有限公司 | Composition for reducing fish catching and transporting stress and preparation method and application thereof |
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- 2022-03-29 CN CN202210319806.1A patent/CN114847404B/en active Active
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| GB0912705D0 (en) * | 2009-07-22 | 2009-08-26 | Questor Group Ltd C | Open ocean fish farm |
| CN101810848A (en) * | 2009-11-09 | 2010-08-25 | 天津瑞贝特科技发展有限公司 | Traditional Chinese medicinal composite for reducing stress response of aquatic animals and preparation method and application thereof |
| CN111149761A (en) * | 2020-02-26 | 2020-05-15 | 中国水产科学研究院黄海水产研究所 | Method for reducing stress in transportation process of oplegnathus punctatus |
| CN113134049A (en) * | 2021-05-19 | 2021-07-20 | 湖南渔经生物技术有限公司 | Composition for reducing fish catching and transporting stress and preparation method and application thereof |
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