CN114847377A - Polygonatum sibiricum and maca tea substitute beverage as well as preparation method and application thereof - Google Patents
Polygonatum sibiricum and maca tea substitute beverage as well as preparation method and application thereof Download PDFInfo
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- CN114847377A CN114847377A CN202210313908.2A CN202210313908A CN114847377A CN 114847377 A CN114847377 A CN 114847377A CN 202210313908 A CN202210313908 A CN 202210313908A CN 114847377 A CN114847377 A CN 114847377A
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Abstract
The invention discloses a polygonatum sibiricum and maca tea substitute beverage and a preparation method thereof, wherein maca, polygonatum sibiricum, medlar, mulberry and red date are compounded according to the traditional Chinese medicine theory to prepare the polygonatum sibiricum and maca tea substitute beverage with the effects of regulating immunity and resisting fatigue, so that the polygonatum sibiricum and maca tea substitute beverage not only has better expression on sensory quality, such as color, taste and fragrance, but also increases the enjoyment of tea tasting and drinking; meanwhile, the volume of the tea bag is reduced, and the tea bag is more convenient to package and carry. And the research on the immunity regulation and anti-fatigue effects is carried out by adopting the model organism zebra fish, and the result shows that the polygonatum sibiricum maca tea substitute drink has good immunity regulation and anti-fatigue effects after being taken for a long time. Therefore, the polygonatum sibiricum and maca tea substitute beverage provided by the invention is suitable for popularization and application in the market.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicine tea-substituted drinks, particularly relates to a polygonatum sibiricum-maca tea-substituted drink formula and a preparation method thereof, and particularly relates to a polygonatum sibiricum-maca tea-substituted drink with effects of regulating immunity and resisting fatigue and application thereof.
Background
At present, people pay more and more attention to food, and the need of nutritional value is raised, and people hope to prevent and even cure related diseases by using diet so as to ensure self health by food. The functional food has the effect, is different from the medicine, has no treatment effect, but has the health care function and stronger safety, and is suitable for special people.
In recent years, the concept of homology of medicine and food is widely concerned, and mild hypertension, hyperlipidemia and hyperglycemia, for example, liver and kidney can expel toxin for the body; excessive fat heat is accumulated in the body, lifestyle diseases such as fatty liver, partial cardiovascular and cerebrovascular diseases, diabetes, gout and the like are in the initial stage through balanced diet and moderate exercise, at present, the treatment of the diseases is biased to oral western medicines, the kidney metabolism is aggravated after long-term administration, the symptoms such as liver and kidney injury, muscular soreness, osteoporosis, vitamin deficiency and the like are caused, the life quality of a patient is influenced, and other adverse reactions are easily caused.
Therefore, the problem to be solved by the technical personnel in the field is how to develop the polygonatum sibiricum maca tea substitute beverage which has obvious effect, can be drunk for a long time and has the effects of assisting in regulating immunity and resisting fatigue.
Disclosure of Invention
In view of the above, the invention aims to provide a polygonatum sibiricum and maca tea substitute beverage, a preparation method thereof and application thereof in preparation of health food, aiming at the problems in the prior art.
In order to achieve the purpose, the invention adopts the following technical scheme:
a polygonatum sibiricum and maca tea substitute beverage is prepared from the following raw materials in parts by weight:
the invention discloses a polygonatum sibiricum and maca tea substitute beverage which is prepared by compounding maca, polygonatum sibiricum, medlar, mulberry and red date according to the theory of traditional Chinese medicine, has excellent expression on sensory quality such as color, taste and fragrance, is reduced by a granulation preparation method, increases the enjoyment of tea drink, and can achieve the effects of assisting in regulating immunity and resisting fatigue after long-term administration. Therefore, the polygonatum sibiricum and maca tea substitute beverage provided by the invention is suitable for popularization and application.
In addition, modern researches show that raw materials such as maca, rhizoma polygonati, medlar, mulberry, red date and the like which serve as raw materials have good effects on tonifying kidney and strengthening yang, tonifying middle-jiao and Qi, clearing liver and improving vision and the like. Wherein the content of the first and second substances,
the alkaloid components in maca have the effects of resisting bacteria, viruses, hemagglutination, pain, inflammation, tumors and cardiovascular diseases, strengthening the immune system, improving the sub-health state and the like;
rhizoma polygonati is taken as a medicine by roots and stems, has the functions of tonifying qi and yin, strengthening spleen, moistening lung and tonifying kidney, is an ideal tonic product for the old, and has no side effect possibly caused by a product for greatly tonifying, warming and drying; and has effects of resisting anoxia, relieving fatigue, and resisting aging; in addition, rhizoma Polygonati also has effects of lowering blood pressure and reducing blood sugar, and can increase coronary artery blood flow, reduce blood lipid and delay atherosclerosis;
the medlar is also a good medicine for strengthening body resistance and consolidating constitution, generating essence and replenishing marrow, nourishing yin and tonifying kidney, tonifying qi and soothing nerves, building up body and delaying senility, and has obvious curative effects on chronic hepatitis, central retinitis, optic atrophy and the like; has strong improving effects on resisting tumor, protecting liver, lowering blood pressure, and aging diseases caused by organ deterioration of the elderly; the medlar has obvious inhibiting effect on cancer cells in vitro, and can be used for preventing the diffusion of the cancer cells and enhancing the immunologic function of a human body;
mulberry is sweet in taste and cold in nature, has the functions of nourishing yin and supplementing blood through heart, liver and kidney channels, and can be used for treating yin deficiency, little body fluid, insomnia and the like. Modern medical clinical evidence: the mulberry has good effects of nourishing heart, liver and kidney, nourishing blood and dispelling wind. Has obvious curative effects on reducing blood fat, relieving neurasthenia, arteriosclerosis, sexual function weakness, deafness and dim eyesight, early white beard and hair, internal heat diabetes, blood deficiency constipation and rheumatic arthralgia;
the cyclic adenosine monophosphate contained in the red dates is an essential component for energy metabolism of human cells, can enhance muscle strength, eliminate fatigue, dilate blood vessels, increase myocardial contractility and improve myocardial nutrition, and has good effects of preventing and treating cardiovascular system diseases.
Therefore, by combining the effects of the raw materials, the invention prepares the sealwort and maca tea substitute beverage with the effects of regulating immunity and resisting fatigue by compounding maca, sealwort, medlar, mulberry and red date, not only has better expression on sensory quality, such as color, taste and fragrance, but also increases the enjoyment of tea drink; meanwhile, the volume of the tea bag is reduced, and the tea bag is more convenient to package and carry.
In addition, in the prior art, although the common usage amount of each traditional Chinese medicine is known, the prescription is an organic whole prepared by combining a plurality of medicines according to diseases, the drug effect of the prescription is not equal to the simple superposition of the medicines on the basis of the common usage amount, the dosage of each traditional Chinese medicine in the prescription can not be determined according to the common usage amount of each traditional Chinese medicine, the dosage and the proportion of the medicines are determined by various considerations such as the characteristics of the medicines and monarch, minister, assistant and guide compatibility, and the like, and the prescription can not be obtained by experimental means such as a comparison method, an orthogonal method and the like simply and preferably.
In addition, the invention also provides a preparation method of the polygonatum sibiricum maca tea substitute beverage, which specifically comprises the following steps:
(1) selecting and cleaning dried raw materials of maca, rhizoma polygonati, medlar, mulberry and red date for later use;
(2) respectively crushing and sieving the raw materials obtained in the step (1) for later use;
(3) weighing the materials treated in the step (2) according to the weight part ratio as claimed in claim 1, and uniformly mixing to obtain a mixture;
(4) extruding and granulating the mixture, and then drying, crushing and sieving to obtain granules;
(5) weighing and bagging the granules according to the specification to obtain the polygonatum sibiricum and maca tea substitute beverage.
Preferably, the raw materials in the step (2) are respectively crushed and sieved by a sieve with 10-24 meshes, the crushed and sieved mesh number after extrusion granulation in the step (4) is 10-50 meshes, and the particle size of the prepared particles is 0.5-2 mm.
Preferably, in the step (4), the drying temperature is 55-65 ℃ and the drying time is 0.5-1.5 h.
The invention also aims to provide application of the polygonatum sibiricum and maca tea substitute beverage in preparation of health-care food.
The sealwort and maca tea substitute beverage disclosed by the invention can be used as a dietary supplement or a health food raw material and is used for regulating immunity and resisting fatigue.
Specifically, the health food is prepared by taking the polygonatum sibiricum maca tea substitute beverage as a basic raw material, sequentially extracting, concentrating, drying and adding auxiliary materials; the dosage form of the health food comprises powder, granules, tablets or capsules.
According to the technical scheme, compared with the prior art, the polygonatum sibiricum and maca tea substitute beverage and the preparation method and application thereof provided by the invention have the following excellent effects:
the sealwort and maca tea substitute disclosed by the invention defines the functional effects and effective functional components of maca, sealwort, medlar, mulberry and red date, and forms a tea substitute for assisting in regulating immunity and resisting fatigue according to the theory of 'monarch, minister, assistant and guide' of traditional Chinese medicine, so that the drinking taste is optimized; the preparation method of the granules also reduces the volume of the tea substitute drink, and is more convenient to carry and take. Not only has better expression on sensory quality such as color, luster, taste and aroma, but also increases the enjoyment of tea tasting and is suitable for popularization and application.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 shows the evaluation of the immunoregulatory activity of the polygonatum sibiricum maca tea substitute beverage.
Fig. 2 is an anti-fatigue activity evaluation of the polygonatum sibiricum maca tea substitute beverage.
Detailed Description
The technical solutions disclosed in the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment of the invention discloses a polygonatum sibiricum and maca tea substitute beverage which has an obvious effect, can be drunk for a long time and has the functions of regulating immunity and resisting fatigue.
The present invention will be further illustrated by the following examples for better understanding, but the invention is not to be construed as being limited thereto, and certain insubstantial modifications and adaptations of the invention by those skilled in the art based on the foregoing disclosure are intended to be included within the scope of the invention.
Example 1:
the sealwort and maca tea substitute drink with the effects of regulating immunity and resisting fatigue is prepared from the following raw materials in parts by weight:
3 parts of maca, 1 part of rhizoma polygonati, 1 part of medlar, 1 part of mulberry and 0.5 part of red date.
The preparation method of the polygonatum sibiricum and maca tea substitute beverage with the effects of assisting in regulating immunity and resisting fatigue specifically comprises the following steps:
(1) selecting and cleaning dried raw materials of maca, rhizoma polygonati, medlar, mulberry and red date for later use;
(2) respectively crushing the raw materials obtained in the step (1), and sieving maca, rhizoma polygonati, medlar, mulberry and red dates by a sieve of 10-24 meshes for later use;
(3) weighing and uniformly mixing the maca, the sealwort, the medlar, the mulberry and the red date processed in the step (2) according to the weight part ratio of claim 1;
(4) putting the uniformly mixed materials in the step (3) into a flat film granulator for extrusion granulation, drying in an oven (temperature: 60 ℃ for 1h), taking out, crushing and sieving with a 10-50 mesh sieve to obtain particles with the particle size of 0.5-2 mm;
(5) weighing and bagging according to the specification, wherein 5.0g of the rhizoma polygonati and maca tea substitute beverage with the effects of auxiliary immunity regulation and fatigue resistance can be obtained in each bag.
Example 2:
the sealwort and maca tea substitute drink with the effects of assisting in regulating immunity and resisting fatigue is prepared from the following raw materials in parts by weight:
3 parts of maca, 2 parts of rhizoma polygonati, 2 parts of medlar, 2 parts of mulberry and 1 part of red date.
The preparation method of the polygonatum sibiricum and maca tea substitute beverage with the effects of assisting in regulating immunity and resisting fatigue specifically comprises the following steps:
(1) selecting and cleaning dried raw materials of maca, rhizoma polygonati, medlar, mulberry and red date for later use;
(2) respectively crushing the raw materials obtained in the step (1), and sieving maca, rhizoma polygonati, medlar, mulberry and red dates by a sieve of 10-24 meshes for later use;
(3) weighing and uniformly mixing the maca, the sealwort, the medlar, the mulberry and the red date processed in the step (2) according to the weight part ratio of claim 1;
(4) putting the uniformly mixed materials in the step (3) into a flat film granulator for extrusion granulation, drying in an oven (temperature: 60 ℃ for 1h), taking out, crushing and sieving with a 10-50 mesh sieve to obtain particles with the particle size of 0.5-2 mm;
(5) weighing and bagging according to the specification, wherein 5.0g of the rhizoma polygonati and maca tea substitute beverage with the effects of auxiliary immunity regulation and fatigue resistance can be obtained in each bag.
Example 3:
the sealwort and maca tea substitute drink with the effects of assisting in regulating immunity and resisting fatigue is prepared from the following raw materials in parts by weight:
5 parts of maca, 3 parts of rhizoma polygonati, 3 parts of medlar, 3 parts of mulberry and 1 part of red date.
The preparation method of the polygonatum sibiricum and maca tea substitute beverage with the effects of assisting in regulating immunity and resisting fatigue specifically comprises the following steps:
(1) selecting and cleaning dried raw materials of maca, rhizoma polygonati, medlar, mulberry and red date for later use;
(2) respectively crushing the raw materials obtained in the step (1), and sieving maca, rhizoma polygonati, medlar, mulberry and red dates by a sieve of 10-24 meshes for later use;
(3) weighing and uniformly mixing the maca, the sealwort, the medlar, the mulberry and the red date processed in the step (2) according to the weight part ratio of claim 1;
(4) putting the uniformly mixed materials in the step (3) into a flat film granulator for extrusion granulation, drying in an oven (temperature: 60 ℃ for 1h), taking out, crushing and sieving with a 10-50 mesh sieve to obtain particles with the particle size of 0.5-2 mm;
(5) weighing and bagging according to the specification, wherein 5.0g of the rhizoma polygonati and maca tea substitute beverage with the effects of auxiliary immunity regulation and fatigue resistance can be obtained in each bag.
The inventive content is not limited to the content of the above-mentioned embodiments, wherein combinations of one or several of the embodiments may also achieve the object of the invention.
To further verify the excellent effects of the present invention, the inventors also conducted the following experiments:
test one:
and evaluating the immunity regulation and fatigue resistance of the sealwort and maca tea by using a zebra fish model.
The method comprises the following steps: weighing the medicinal tea prepared in the embodiment 2, soaking and extracting in water bath, and freeze-drying; selecting transgenic zebra fish with 2dpf immune cell fluorescence labeling, establishing an immune hypofunction model by using vinorelbine, performing anesthesia photographing after medicinal tea treatment, and counting the fluorescence intensity of tail immune cells; selecting 5dpf wild AB line zebra fish, placing the zebra fish into an anoxic box, performing hypoxia treatment to cause a fatigue model, and detecting the total swimming distance and the average swimming speed after medicinal tea treatment.
Experimental results show that 50-200 mu g/mL of the polygonatum-maca medicinal tea extract has an immunoregulation effect on the zebra fish, and 25-200 mu g/mL of the polygonatum-maca medicinal tea extract has an anti-fatigue effect on the zebra fish.
Materials and instruments
1) Material
The zebra fish is provided for a zebra fish drug screening platform of biological research institute of academy of sciences in Shandong province; samples were provided by Shanxi Shadong pharmaceutical Co.
2) Instrument for measuring the position of a moving object
Model AXIO zoom.v16 fluorescence microscope (zeiss, germany), FSX100 fluorescence microscope (olympus, japan), zebra fish breeding system (eisheng technologies ltd, beijing).
(II) method
1) Extraction of herb tea
Weighing medicinal tea, adding 5 times of purified water, placing in a 95 deg.C water bath, extracting for 1h, filtering or centrifuging, collecting supernatant, concentrating under reduced pressure with a rotary evaporator, concentrating to a certain volume, and transferring to a freeze-drying machine for freeze-drying.
2) Zebrafish embryo harvest
And (4) separately feeding male and female zebra fishes, alternately lighting for 14 h/dark for 10h, and regularly feeding artificial granular bait and newly hatched artemia nauplius. And (3) putting healthy and mature zebra fish into a mating jar according to the female parent and the male parent by the ratio of 1:1 during egg collection, and obtaining fertilized eggs the next 9-10 days. Sterilizing and washing fertilized egg, transferring into water (containing 5.0mM NaCl, 0.1 mM NaCl) for culturing zebra fish embryo7mM KCl，0.4mM CaCl 2 ，0.16mM MgSO 4 ) Culturing at 28 deg.C under controlled light.
3) Evaluation of modulating immune Activity
Transgenic zebrafish (Tg (lyz: DSRED2)) fluorescently labeled with 2dpf immune cells were randomly transferred to 24-well plates, and 12 pieces of each well were divided into a blank control group, a model group and a herb tea treatment group. E3 culture water is given to the blank control group, 150 mu g/mL vinorelbine solution is respectively given to the other groups (model group and herb tea treatment group) to establish a zebra fish immune hypofunction model, and 100, 200 and 400 mu g/mL herb tea solution is respectively given to the herb tea treatment group. After the culture box is cultured for 24 hours, anesthesia and photographing are carried out, and the fluorescence intensity of tail immune cells is counted.
4) Evaluation of anti-fatigue Activity
5dpf of healthy AB-line zebra fish is randomly transferred to a 24-well plate, 16 zebra fish in each well are divided into a blank control group, a model group and a herb tea treatment group. The blank control group and the model group are given with normal oxygen culture water, and the herb tea treatment group is given with 100, 200, 400 and 800 mug/mL herb tea extract solution respectively. And placing the model group and the medicinal tea treatment group in an anaerobic condition to cause a fatigue model, culturing for 6 hours in an incubator, transferring each group of zebra fishes to a 48-hole plate, placing the zebra fishes in an ethology detection box, standing for 10 minutes, collecting 10-minute ethology data, and counting the total moving distance and the average moving speed.
(III) results of the experiment
1) Evaluation of modulating immune Activity
FIG. 1 results of modulated immune activity evaluation. A: generating a fluorescent picture of the model by the zebra fish immune cells; the solid line delineating the area of immune cell fluorescence in the figure; the arrow indicates an immune cell; a blank control group (Ctrl), a vinorelbine (NVB) model group, a Levamisole Hydrochloride (LH) positive medicine group, a polygonatum and maca medicine tea (YC)50 mu g/mL medicine feeding group, a polygonatum and maca medicine tea 100 mu g/mL medicine feeding group and a polygonatum and maca medicine tea 200 mu g/mL medicine feeding group are arranged from top to bottom in sequence. B: the rhizoma polygonati and maca herbal tea extracts with different dosages have immunoregulation activity on zebra fish. ****: p < 0.001vs blank control, ####': p < 0.001vs model group.
As shown in fig. 1, the number of immune cells in zebra fish in the vinorelbine treatment group (model group) is significantly reduced compared with that in the blank control group, and the number of immune cells in zebra fish in the levamisole hydrochloride (positive drug group) and medicinal tea extract treatment group is significantly increased compared with that in the model group, which indicates that the polygonatum sibiricum and maca tea extract has immunoregulation activity on zebra fish at 50-200 μ g/mL.
2) Evaluation of anti-fatigue Activity
FIG. 2 shows the results of the evaluation of anti-fatigue activity. A: a zebra fish behavioral swimming trail diagram; in the figure, black represents low speed, green represents medium speed; control is blank Control group, Model is anoxia Model group, RC-1 is rhodiola rosea positive medicine group of 1 μ g/mL, YC-25, YC-50, YC-100 and YC-200 are administration groups of rhizoma Polygonati and maca tea of 25 μ g/mL, 50 μ g/mL, 100 μ g/mL and 200 μ g/mL respectively. B: influence of the polygonatum sibiricum and maca tea extract on the swimming distance of the zebra fish. C: influence of the polygonatum sibiricum and maca tea extract on the swimming speed of the zebra fish. **: p < 0.01vs blank, ##:: p < 0.01vs model group, # # ##:: p < 0.001vs model group.
As shown in fig. 2, the total moving distance and the average moving speed of zebra fish in the hypoxia treatment group (model group) are both significantly reduced compared with those in the blank control group, the total moving distance and the average moving speed of rhodiola rosea in the treatment group (positive medicine group) are both significantly increased compared with those in the model group, and the total moving distance and the average moving speed of the herb tea extract treatment group are both significantly increased compared with those in the model group, which indicates that the polygonatum-maca herb tea extract of 25-200 mug/mL has an anti-fatigue effect on zebra fish.
(IV) conclusion
The experiment shows that 50-200 mu g/mL of the polygonatum sibiricum and maca tea extract has immunoregulation activity on a zebra fish immune hypofunction model caused by vinorelbine, and 25-200 mu g/mL of the polygonatum sibiricum and maca medicinal tea extract has an anti-fatigue effect on a zebra fish fatigue model caused by hypoxia.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (6)
1. The sealwort and maca tea substitute beverage is characterized by comprising the following raw materials in parts by weight:
2. the preparation method of the polygonatum sibiricum maca tea substitute beverage according to claim 1, which is characterized by comprising the following steps:
(1) selecting and cleaning dried raw materials of maca, rhizoma polygonati, medlar, mulberry and red date for later use;
(2) respectively crushing and sieving the raw materials obtained in the step (1) for later use;
(3) weighing the materials treated in the step (2) according to the weight part ratio as claimed in claim 1, and uniformly mixing to obtain a mixture;
(4) extruding and granulating the mixture, and then drying, crushing and sieving to prepare granules;
(5) weighing and bagging the granules according to the specification to obtain the polygonatum sibiricum and maca tea substitute beverage.
3. The preparation method of the sealwort and maca tea substitute beverage according to claim 2, wherein the raw materials in the step (2) are respectively crushed and then sieved by a sieve with 10-24 meshes, the crushed and sieved meshes in the step (4) after extrusion granulation are 10-50 meshes, and the particle size of the prepared particles is 0.5-2 mm.
4. The preparation method of the sealwort and maca tea substitute beverage according to claim 2 or 3, wherein in the step (4), the drying temperature is 55-65 ℃, and the drying time is 0.5-1.5 h.
5. Use of the polygonatum sibiricum-maca tea substitute beverage according to claim 1 or the polygonatum sibiricum-maca tea substitute beverage prepared by the method according to claim 2 in preparation of health food.
6. The application of the health food as claimed in claim 5, wherein the health food is prepared by taking the polygonatum sibiricum maca tea substitute beverage as a basic raw material, sequentially extracting, concentrating and drying the raw material, and adding auxiliary materials; the dosage form of the health food comprises powder, granules, tablets or capsules.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011019210A2 (en) * | 2009-08-11 | 2011-02-17 | Kim Younjin | Dietary supplement, feed and pharmaceutical composition containing chia seed and maca, and preparation method thereof |
| CN106135570A (en) * | 2016-06-28 | 2016-11-23 | 禹州市鼎信中药科技有限公司 | A kind of Lepidinm meyenii Walp Radix Ginseng alternative tea formula and production technology thereof |
| CN111602725A (en) * | 2019-02-25 | 2020-09-01 | 海南食在生物科技有限公司 | Maca ginseng multi-treasure tea |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011019210A2 (en) * | 2009-08-11 | 2011-02-17 | Kim Younjin | Dietary supplement, feed and pharmaceutical composition containing chia seed and maca, and preparation method thereof |
| CN106135570A (en) * | 2016-06-28 | 2016-11-23 | 禹州市鼎信中药科技有限公司 | A kind of Lepidinm meyenii Walp Radix Ginseng alternative tea formula and production technology thereof |
| CN111602725A (en) * | 2019-02-25 | 2020-09-01 | 海南食在生物科技有限公司 | Maca ginseng multi-treasure tea |
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