CN107596013A - A kind of extracting method of maca and its male health-care product of preparation - Google Patents
A kind of extracting method of maca and its male health-care product of preparation Download PDFInfo
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Abstract
The present invention provides a kind of male health-care product prepared with maca, and it is prepared by the raw material extraction of following parts by weight：20 30 parts of maca, 15 25 parts of lycium ruthenicum, 10 18 parts of black pine, 10 20 parts of peristrophe, 8 12 parts of geum aleppicum, and corresponding preparation method is employed, the present invention has the effect of improving immunity and suppressing platelet aggregation.
Description
Technical field
The invention belongs to technical field of health care food, and in particular to a kind of extracting method of maca and its male of preparation protect
Strong product.
Background technology
Maca (scientific name：Lepidium meyenii Walp.), it is (Spanish：Maca).It is to originate in South America Andean
A kind of crucifer on mountain range.Leaf is oval, rhizome likeness in form turnip radish, edible, is a kind of natural food, nutrition
Composition enriches, and has the reputation of " South America ginseng ".The hypocotyl of agate card may be in golden or faint yellow, red, purple, blueness, black
Color or green.Flaxen most common, and shape, taste are preferably also.Agate card is rich in high unit nutrient, has nourishing to human body
The function of salubrity.Agate card originates in High aititude mountain area, suitably in High aititude, low latitudes, high day and night temperature, subacidity sandy loam, sunlight
Grown in sufficient soil.Plantation area is distributed mainly on the Yulong Xueshan of South America Andes and Chinese yunnan Lijing
There is suitable kind of soil of larger area area, this two big main producing region.And the area such as other areas of Chinese yunnan and Xinjiang, Tibet also has
A small amount of plantation.But occur that mood is irritated when maca is taken, emit the excessive internal heat symptom such as small pox on the face, and few compounds produce
The research of product compatibility.
The content of the invention
Form is described in further detail again to the above of the present invention by the following examples, but should not manage this
The scope solved as the above-mentioned theme of the present invention is only limitted to following embodiment, and all technologies for being realized based on the above of the present invention are equal
Belong to the scope of the present invention.
Goal of the invention：In order to solve the above problems, the invention provides a kind of extracting method of maca and its man of preparation
Sex-health products.
Technical scheme：A kind of male health-care product prepared with maca, it is prepared into by the raw material extraction of following parts by weight
Arrive：Maca 20-30 parts, lycium ruthenicum 15-25 parts, black pine 10-18 parts, peristrophe 10-20 parts, geum aleppicum 8-12
Part.
The male health-care product prepared with maca, it is prepared by the raw material extraction of following parts by weight：Maca 25
Part, 20 parts of lycium ruthenicum, 14 parts of black pine, 15 parts of peristrophe, 10 parts of geum aleppicum.
The male health-care product prepared with maca, preparation method comprise the following steps：Take maca to crush, add maca medicine
70% ethanol of 10 times of material gross dry weight, refluxing extraction 2 times, 2 hours every time, filtering, merging filtrate simultaneously carried out concentration removing second
Alcohol, maca ethanol extract is obtained, standby, filter residue is dried at 60 DEG C, is crushed, standby；Filtered after toward gained maca extract
The water of 2 times of filter residue weight is added in slag, the use of sodium citrate solution regulation system pH value is 5.0, adds the 0.8% of filter residue weight
Enzyme activity is 30000u/g cellulase, the shaking bath 3.0h at 50 DEG C, filtering, obtains maca enzymolysis liquid, merges maca ethanol
Extract solution and maca enzymolysis liquid, concentration, are spray-dried to obtain Maca extract；Remaining four taste Chinese medicine is taken to add the 5-15 times of water measured,
Decoct three times, each 1-3 hours, merge extract solution, stand 12-24 hours, remove precipitation, take supernatant, filter, filtrate concentration
Into the clear cream that relative density is 1.08-1.12 at 60 DEG C, let cool；95% ethanol is added in the clear cream of gained, reaches alcohol content
50%, stirring, 12-24 hours are stood, take supernatant, filtered, continuing stirring 95% ethanol of addition in filtrate reaches alcohol content
70%, 12-24 hours are stood, take supernatant, are filtered, after filtrate recycling ethanol, are concentrated into thick medicinal extract, is dried in vacuo, produces institute
Four taste Chinese medical extracts are stated, merge Maca extract and four taste Chinese medical extracts, auxiliary material is added, is prepared into solid pharmaceutical preparation.
The male health-care product prepared with maca, solid pharmaceutical preparation is tablet or capsule or granule.
It is described to prepare the application in treating hypoimmunity health products with male health-care product prepared by maca.
It is described to prepare the application in suppressing platelet aggregation health products with male health-care product prepared by maca.
Peristrophe, Classification system：Peristrophe japonica (Thunb.) Bremek., Acanthaceae, Premna microphylla Turez
Category herbaceous plant, up to 50 centimetres.Leaf egg shape square is circular, and poly- umbrella flower basidixed or axillary are in top axil, and filigree is elongated, flower pesticide quilt
Long bristle, capsule sparsely grow pubescence, placenta is not upspring during cracking, and seed has small verruca.It is distributed on the south the basin of farm labourer river
Various regions.It is born in the dark and damp places such as hillside, sylvan life, roadside, small stream side.Nature and flavor are pungent, slight bitter, cool.Inducing sweat and dispelling exogenous evils, clearing heat and detoxicating, antispastic.With
In flu, abscess of throat, diphtheria, children's indigestion, infantile hyperpyrexia, carbuncle furuncle poison, venomous snake bite.The present invention is entered with herb
Medicine.
Geum aleppicum is rose family avens geum aleppicum Geum aleppicum Jacq., with herb or
Root is used as medicine.Summer is excavated, and chopping is dried.It is clearing heat and detoxicating, swelling and pain relieving.For enteritis, dysentery, child convulsion, pain in waist and lower extremities, bruise
Damage, irregular menstruation, leukorrhea；Furunculosis, carbuncle swells are controlled in external application.The present invention is with all herbal medicine.
Beneficial effect：Maca of the present invention improves sexual function, fatigue-relieving, improves sleep, nourishing and fit keeping function, beautifying face and moistering lotion, black
Matrimony vine strengthening by means of tonics, it is black pine anti-aging, antifatigue, it is altogether monarch drug in a prescription, peristrophe thermal detoxification, antispastic, geum aleppicum is clear
Thermal detoxification, swelling and pain relieving are ministerial drug, can improve immunity entirely, separately find that it can suppress platelet aggregation.
Embodiment
Form is described in further detail again to the above of the present invention by the following examples, but should not manage this
The scope solved as the above-mentioned theme of the present invention is only limitted to following embodiment, and all technologies for being realized based on the above of the present invention are equal
Belong to the scope of the present invention.
Embodiment 1：Take 20 parts of maca, 25 parts of lycium ruthenicum, 10 parts of black pine, 20 parts of peristrophe, geum aleppicum 8
Part, preparation method comprises the following steps：Take maca to crush, add 70% ethanol of 10 times of maca medicinal material gross dry weight, backflow carries
Take 2 times, 2 hours every time, filtering, merging filtrate simultaneously carried out concentration removing ethanol, obtains maca ethanol extract, standby, filter residue
Dried, crushed at 60 DEG C, it is standby；The water of 2 times of filter residue weight is added after toward gained maca extract in filter residue, uses lemon
Acid sodium solution regulation system pH value is 5.0, and 0.8% enzyme activity for adding filter residue weight is 30000u/g cellulase, at 50 DEG C
Lower shaking bath 3.0h, filtering, maca enzymolysis liquid is obtained, merge maca ethanol extract and maca enzymolysis liquid, concentrated, spray drying
Obtain Maca extract；Take remaining four taste Chinese medicine to add the water of 5 times of amounts, decoct three times, 3 hours every time, merge extract solution, stand 12
Hour, precipitation is removed, takes supernatant, is filtered, filtrate is condensed into the clear cream that relative density is at 60 DEG C 1.12, lets cool；Gained
95% ethanol is added in clear cream, alcohol content is reached 50%, stirring, stands 12 hours, take supernatant, filter, continue in filtrate
Stirring, which adds 95% ethanol, makes alcohol content reach 70%, stands 24 hours, takes supernatant, filters, after filtrate recycling ethanol, concentration
To thick medicinal extract, vacuum drying, the four tastes Chinese medical extract is produced, merge Maca extract and four taste Chinese medical extracts, addition
Auxiliary material, prepare piece agent.
Embodiment 2：Take 30 parts of maca, 15 parts of lycium ruthenicum, 18 parts of black pine, 10 parts of peristrophe, geum aleppicum 12
Part, preparation method comprises the following steps：Take maca to crush, add 70% ethanol of 10 times of maca medicinal material gross dry weight, backflow carries
Take 2 times, 2 hours every time, filtering, merging filtrate simultaneously carried out concentration removing ethanol, obtains maca ethanol extract, standby, filter residue
Dried, crushed at 60 DEG C, it is standby；The water of 2 times of filter residue weight is added after toward gained maca extract in filter residue, uses lemon
Acid sodium solution regulation system pH value is 5.0, and 0.8% enzyme activity for adding filter residue weight is 30000u/g cellulase, at 50 DEG C
Lower shaking bath 3.0h, filtering, maca enzymolysis liquid is obtained, merge maca ethanol extract and maca enzymolysis liquid, concentrated, spray drying
Obtain Maca extract；Take remaining four taste Chinese medicine to add the water of 15 times of amounts, decoct three times, 1 hour every time, merge extract solution, stand
24 hours, precipitation is removed, takes supernatant, filtered, filtrate is condensed into the clear cream that relative density is at 60 DEG C 1.08, lets cool；Gained
Clear cream in add 95% ethanol, alcohol content is reached 50%, stirring, stand 24 hours, take supernatant, filter, filtrate relaying
Continuous stirring, which adds 95% ethanol, makes alcohol content reach 70%, stands 12 hours, takes supernatant, filters, dense after filtrate recycling ethanol
Thick medicinal extract is reduced to, is dried in vacuo, produces the four tastes Chinese medical extract, merges Maca extract and four taste Chinese medical extracts, adds
Add auxiliary material, be prepared into capsule.
Embodiment 3：Take 25 parts of maca, 20 parts of lycium ruthenicum, 14 parts of black pine, 15 parts of peristrophe, geum aleppicum 10
Part, preparation method comprises the following steps：Take maca to crush, add 70% ethanol of 10 times of maca medicinal material gross dry weight, backflow carries
Take 2 times, 2 hours every time, filtering, merging filtrate simultaneously carried out concentration removing ethanol, obtains maca ethanol extract, standby, filter residue
Dried, crushed at 60 DEG C, it is standby；The water of 2 times of filter residue weight is added after toward gained maca extract in filter residue, uses lemon
Acid sodium solution regulation system pH value is 5.0, and 0.8% enzyme activity for adding filter residue weight is 30000u/g cellulase, at 50 DEG C
Lower shaking bath 3.0h, filtering, maca enzymolysis liquid is obtained, merge maca ethanol extract and maca enzymolysis liquid, concentrated, spray drying
Obtain Maca extract；Take remaining four taste Chinese medicine to add the water of 10 times of amounts, decoct three times, 2 hours every time, merge extract solution, stand
18 hours, precipitation is removed, takes supernatant, filtered, filtrate is condensed into the clear cream that relative density is at 60 DEG C 1.10, lets cool；Gained
Clear cream in add 95% ethanol, alcohol content is reached 50%, stirring, stand 18 hours, take supernatant, filter, filtrate relaying
Continuous stirring, which adds 95% ethanol, makes alcohol content reach 70%, stands 18 hours, takes supernatant, filters, dense after filtrate recycling ethanol
Thick medicinal extract is reduced to, is dried in vacuo, produces the four tastes Chinese medical extract, merges Maca extract and four taste Chinese medical extracts, adds
Add auxiliary material, be prepared into granule.
Embodiment 4：The present invention improves the pharmacodynamic study of immunity
Experiment purpose：The maca health products prepared with 1-3 of the embodiment of the present invention, gavage mouse 30d, each 0.1ml/10g,
Compare difference of the of the invention and normal group mouse by the ConA splenic lymphocyte proliferations induced, to judge whether the present invention strengthens
Splenic lymphocyte proliferation.
Experimental animal：Kunming mouse, male, body weight 18-22g, our company provide.
Experimental agents：Prepared by above-described embodiment 1-3 preparation method.
Experimental procedure：Mouse, it is grouped by body weight stratified random, every group 10.It is as shown in the table, the feedwater of normal group gavage；
Levamisole hydrochloride group gavage levamisole hydrochloride；Embodiment 1-3 gavages 2gkg-1, each group continuous gavage administration 30d.It is sterile
Spleen is taken, according to individual cells suspension is conventionally produced, adjustment cell concentration is 5 × 105Individual/mL.Splenocyte suspension is added in 24
In orifice plate, per hole 1mL, every animal sets two multiple holes, and a hole is normally cultivated and (adds 50uL PRMI1640 nutrient solutions)；One hole adds
50uL concanavalin As (ConA) liquid (final concentration of 4.76ug/mL).It is placed in 5%CO2, 37 DEG C of CO248h is cultivated in incubator.Experiment
4h before end, supernatant 700uL is sucked per hole, add the PRMI1640 nutrient solutions that 700uL is free of hyclone, while per hole
Add 50uL 5mg/mL MTT liquid.After culture terminates, add 1mL acid isopropyl alcohol per hole, carefully blow and beat uniformly to the knot of purple
Brilliant all dissolvings.It is dispensed into before detection in 96 well culture plates, makees 4 multiple holes per hole, its optical density is determined at 570nm wavelength
Value.
Experimental result：
The present invention to spleen lymphocyte proliferation influence (N=12)
Note：Compared with normal group★★P ＜ 0.01,★P ＜ 0.05.
Conclusion：Proliferation rate after 1-3 maca health products mouse spleen lymphocytes of the embodiment of the present invention are induced by ConA turns
Change value is higher than normal group mouse, illustrates that the present invention has and improves immunity.
Embodiment 5：Influence of the present invention to rat platelet aggregation function
Animal：Cleaning grade Sprague-Dawley rats, male, body weight 200g-250g, our company's Experimental Animal Center.
Method and result：Animal is randomly divided into blank control group (waiting capacity solvent), aspirin group (ASA, 50mg/
Kg), clopidogrel group (7mg/kg), maca health products 20mg/kg groups of the present invention, maca health products 40mg/kg groups of the present invention, sheet
Invention maca health products 80mg/kg groups, maca health products are prepared using the method for above-described embodiment 3, every group 8, gastric infusion, and 1
Secondary/d, continuous 5d.The 1h after last dose, with 3% yellow Jackets difference anesthetized rat (30mg/kg), adopted through abdominal aorta
Blood, with 3.8% liquor sodii citratis anti-freezing (blood：Anti-coagulants=9:1), 1000r/min, separation platelet rich plasma (PRP), remain
Remaining part point centrifuges 15min with 3000r/min again, separation platelet poor plasma (PPP), is with ADP (252umol/L) by turbidimetry
Derivant, platelet aggregation rate in 5min is determined with LBY-NJ blood pools instrument, and calculate platelet aggregation suppression as follows
Rate processed, data represent that the t between group, which is examined, carries out statistical procedures, the results are shown in Table with %.
L-Arginine=(blank control platelet aggregation rate %- delivery tubes platelet aggregation rate %)/blank
Compare platelet aggregation rate %*100%
It can be seen from experimental result, each dosage group of maca health products of the present invention can substantially suppress internal platelet aggregation, medicine
Effect maintains an equal level with positive drug aspirin and clopidogrel.
Maca health products of the present invention are to hematoblastic aggregation inhibiting rate (n=8)
Compared with blank group, * * P<0.001*P<0.01
Conclusion：Using aspirin and clopidogrel as positive drug, it is experimentally confirmed, maca health products of the present invention can be bright
Aobvious suppression platelet aggregation, maintains an equal level with positive drug, can be used for preparing medicament for resisting platelet aggregation, it is small that the present invention is also resistant to blood
Plate is assembled.
Embodiment 6：Maca health product for treating fatigue clinical research of the present invention
General data：Maca health products of the present invention are applied to clinical observation fatigue case 50, wherein man 25, female 25
Example, 46~65 years old age.The course of disease most short person 8 months, most elder was up to 2 years.
Diagnostic criteria：1. main standard：Must possess two：(1) serious weak fatigue, continues at least six moon.(2)
The internal medicine or mental illness for causing fatigue are not found, such as malignant tumour, autoimmune disease, infectious diseases, nerve
Muscle disease, drug habit, poisoning etc..2. objective standard：At least there are following 2：Low-heat；Nonexudativeage sphagitis；Pharynx
Throat's pain.Duration is grown, and neck or axillary lymph knot silght enlargement, there is tenderness.3. minor criteria：It is it is required that at least following
8 kinds of symptoms in symptom：Extensive headache, courbature, arthralgia, heating, sore-throat, neck or axillary gland pain, flesh
Meat is powerless, and the taediumvitae for continuing more than 2~4 hours after slight work, (such as emotional, forgetful, notice is or not Spirit nerve symptoms of disease
Concentration, thinking difficulty, depression etc.) sleep-disorder, the fatigue occurred suddenly etc..After taking excessive internal heat Symptoms be mood it is irritated,
Small pox etc. is emitted on the face.
Test method：Treatment group prepares maca health products by the method for above-described embodiment 3, and specification is every bag 10 grams, 30 days
For a course for the treatment of, one day three bags, it is point early, middle and late that half an hour takes before meals three times.Raw and cold, pungent, stimulation and corruption are avoided eating during medication
Rotten thing.Blank group oral placebo granule, five days is a course for the treatment of, one day three bags, point early, middle and late half an hour before meals three times
Take.Maca is combined extraction with other four tastes Chinese medicines in the preparation method of control group maca health products, that is, takes in five tastes taste
Medicine adds the 5-15 times of water measured, and decocts three times, each 1-3 hours, merges extract solution, stand 12-24 hours, removes precipitation, take
Supernatant, filtration, filtrate are condensed into the clear cream that relative density is 1.08-1.12 at 60 DEG C, let cool；Added in the clear cream of gained
95% ethanol, alcohol content is reached 50%, stirring, stand 12-24 hours, take supernatant, filter, continue stirring in filtrate and add
95% ethanol makes alcohol content reach 70%, stands 12-24 hours, takes supernatant, filters, and after filtrate recycling ethanol, is concentrated into thick
Medicinal extract, vacuum drying, the traditional Chinese medicine of the five flavours extract is produced, add auxiliary material, be prepared into granule.
Criterion of therapeutical effect：Recovery from illness：Clinical symptoms all disappear, and laboratory checks normal.Take a turn for the better：Clinical symptom relief, the state of an illness
Controlled, laboratory examination improvement or normal.It is invalid：No significant improvement or aggravated in clinical symptoms.
Therapeutic effect：It is shown in Table 1.
1 two groups of curative effects of table compare
| Group | Recovery from illness | Effectively | It is invalid | Excessive internal heat patient | Total effective rate (%) |
| Treatment group | 9 | 13 | 3 | 2 | 88 |
| Blank group | 1 | 2 | 22 | 0 | 12 |
| Control group | 7 | 14 | 4 | 23 | 84 |
It can be seen that maca health products produced by the present invention can be antifatigue, and maca uses after special conditions of contract extracts,
The patient populations of excessive internal heat symptom significantly reduce.
Claims (6)
- A kind of 1. male health-care product prepared with maca, it is characterised in that：It is prepared into by the raw material extraction of following parts by weight Arrive：Maca 20-30 parts, lycium ruthenicum 15-25 parts, black pine 10-18 parts, peristrophe 10-20 parts, geum aleppicum 8-12 Part.
- 2. the male health-care product prepared as claimed in claim 1 with maca, it is characterised in that：It by following parts by weight raw material Extraction is prepared：25 parts of maca, 20 parts of lycium ruthenicum, 14 parts of black pine, 15 parts of peristrophe, 10 parts of geum aleppicum.
- 3. the male health-care product prepared as claimed in claim 1 with maca, it is characterised in that：Preparation method comprises the following steps： Take maca to crush, add 70% ethanol of 10 times of maca medicinal material gross dry weight, refluxing extraction 2 times, 2 hours every time, filter, merge Filtrate simultaneously carries out concentration removing ethanol, obtains maca ethanol extract, standby, filter residue is dried at 60 DEG C, is crushed, standby； The water of 2 times of filter residue weight is added after toward gained maca extract in filter residue, is using sodium citrate solution regulation system pH value 5.0,0.8% enzyme activity for adding filter residue weight is 30000u/g cellulase, the shaking bath 3.0h at 50 DEG C, filtering, is obtained Maca enzymolysis liquid, merge maca ethanol extract and maca enzymolysis liquid, concentration, be spray-dried to obtain Maca extract；Take remaining four Taste Chinese medicine adds the 5-15 times of water measured, and decocts three times, each 1-3 hours, merges extract solution, stands 12-24 hours, and it is heavy to remove Form sediment, take supernatant, filter, filtrate is condensed into the clear cream that relative density is 1.08-1.12 at 60 DEG C, lets cool；In the clear cream of gained 95% ethanol is added, alcohol content is reached 50%, stirring, stands 12-24 hours, take supernatant, filter, continue to stir in filtrate Adding 95% ethanol makes alcohol content reach 70%, stands 12-24 hours, takes supernatant, filters, after filtrate recycling ethanol, concentration To thick medicinal extract, vacuum drying, the four tastes Chinese medical extract is produced, merge Maca extract and four taste Chinese medical extracts, addition Auxiliary material, it is prepared into solid pharmaceutical preparation.
- 4. the male health-care product prepared as claimed in claim 3 with maca, it is characterised in that：Solid pharmaceutical preparation is tablet or capsule Or granule.
- 5. the answering in treatment hypoimmunity health products are prepared with male health-care product prepared by maca as claimed in claim 1 With.
- 6. the answering in suppression platelet aggregation health products are prepared with male health-care product prepared by maca as claimed in claim 1 With.
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Application publication date: 20180119 |
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| RJ01 | Rejection of invention patent application after publication |