CN107281390A

CN107281390A - It is a kind of for Chinese medicine composition of health care and preparation method thereof

Info

Publication number: CN107281390A
Application number: CN201610202799.1A
Authority: CN
Inventors: 窦啟玲; 杨青波; 李宇; 袁兵兵; 严尧; 骆弟松
Original assignee: Guizhou Yibai Pharmaceutical Co Ltd
Current assignee: Guizhou Yibai Pharmaceutical Co Ltd
Priority date: 2016-04-05
Filing date: 2016-04-05
Publication date: 2017-10-24

Abstract

The invention provides a kind of for Chinese medicine composition of health care and preparation method thereof, Chinese medicine composition is made up of rhizoma Gastrodiae, Radix Angelicae Sinensis, mulberry fruit, jujube, the Radix Astragali, and the active component for mixing or being extracted by this area conventional extraction techniques that can directly pulverize is combined with pharmaceutically acceptable pharmaceutic adjuvant is made various pharmaceutical dosage forms；With energy strengthen immunity, antifatigue, raising body hypoxia-bearing, hypoglycemic, reducing blood pressure and blood fat, spot-removing beautifying face beauty, raising memory, useful for sleeping, Anshen Bunao, Johnson ＆ Johnson's body-building functions；Compared with prior art, present invention treatment inferior health is diseases related evident in efficacy, and clinical application is safer.

Description

It is a kind of for Chinese medicine composition of health care and preparation method thereof

Technical field

The present invention relates to a kind of for Chinese medicine composition of health care and preparation method thereof, belong to the technology of Chinese medicine Field.

Technical background

With living-pattern preservation, the quickening of rhythm of life, people are in the kownledge economy of Modern High-Speed development Type society, most people generally feel mental burden weight, and study, work, rhythm of life are accelerated, pressure increase, Mood constrains tired strongly fragrant, and the state of mind is chronically at tense situation；In addition bad life style and custom, diet is not Control and inappropriate, work and rest irregular, environment, air, drinking-water source are contaminated, and foodsafety is poor, for a long time Lack physical training, energy is in overdraw state often；Thus caused decrease of memory, agitation are easily angry, easy The sub-health state such as fatigue, reaction slow, immunocompetence low, resistivity is weak, poor sleeping quality occurs therewith； Inferior health causes people's quality of life to decline, and can increase the risk that people suffer from various diseases, if thinked little of, with And come is more serious disease, or even a certain degree of threat is caused to life.

In recent years, with the increasingly raising of people's living standard, the common people start to pursue preferably life matter Amount and the higher general level of the health, to health, the serious hope promoted longevity so that the numerous common people begin with for health care Increasing concern；The transformation of medical model, promotion defence, health care, rehabilitation are combined with drug therapy, Disease is changed into put prevention first based on the treatment, the development of Health-care Foods Industry is greatly promoted；Country Take measures to support and encourage the exploitation of health care's product in policy.

Traditional Chinese medicine health had deep theoretical foundation and practical experience by the development of thousands of years；Traditional Traditional Chinese medicine health care is Chinese nation's understanding human life, safeguards wisdom knot that is healthy and then prolonging life Brilliant and culture rarity；From the point of view of the thinking of the traditional Chinese medical science, inferior health shape be it is cloudy or it is positive contain to decline or both partially partially all decline partially A kind of state, simply do not occurred pole state also, beyond the scope of YIN and YANG maintaining in a changeable equilibrium, but trend is It is aobvious；So can select few without toxic component or adverse reaction, security stronger Chinese medicine makees fine setting negative and positive, It is allowed to revert to the health status of " yin and yang in equilibrium ".

Chinese medicine composition of the present invention is made up of rhizoma Gastrodiae, Radix Angelicae Sinensis, mulberry fruit, jujube, Radix Astragali traditional Chinese medicine of the five flavours； Rhizoma Gastrodiae also known as rhizoma gastrodiae, solely shake sesame, from it is female, close from grass, refreshing grass, yellow moccasin flower, Mokpo, RHIZONA GASTRODIAE, DINGFENGCAO, Bai Longpi etc., is orchid family Gastrodia herbaceos perennial, its rhizome be used as medicine to treat have a dizzy spell, limbs The diseases such as numbness, child convulsion, are rare Chinese medicines；Radix Angelicae Sinensis is the dry root of umbelliferae angelica, warm-natured, taste It is sweet, pungent, enter liver, the heart, the spleen channel, with replenishing and activating blood, menstruction regulating and pain relieving, the function of relaxing bowel；Mulberry fruit is The fruit of Moraceae Morus perennial woody plant mulberry tree, ellipse is long 1-3 centimetres, uneven surface,《This Grass is newly organized》Have in the record of " purple person is first, and red person takes second place, blue or green then unavailable ", mulberry fruit and contain a variety of work( Can property composition, such as rutin, anthocyanidin, RV, with good anti-cancer, anti-aging, antiulcer, It is antiviral to wait effect；Jujube, also known as jujube, dry jujube, Chinese date, originating from China, in China existing more than 8,000 The plantation history in year, is just listed in one of " five fruits " (chestnut, peach, Lee, apricot, jujube), jujube is rich in since ancient times Protein, fat, carbohydrate, carrotene, B family vitamin, vitamin C, citrin and calcium, phosphorus, The nutritional ingredient such as iron and CAMP, wherein ascorbic content comes out at the top in fruit, has dimension to give birth to The laudatory title of plain king, the effect of having a sleepless night is treated with blood-enriching face-nourishing；The Radix Astragali, also known as astragali or northern alpine yarrow, also make astragalus One of or yellow alpine yarrow, conventional Chinese medicine, it is the root of legume astragalus mongolicus or Astragalus membranacus, main product is in China The ground such as Mongolia, Shanxi, Heilungkiang, the Radix Astragali can be divided into：Inner Mongolia Astragalis, Astragalus membranacus, the continuous Radix Astragali, many sequence rocks The Radix Astragali (also known as " red stilbene "), the Japanese Radix Astragali (also known as " and Radix Astragali ").

In recent years, using traditional Chinese medicine individually or compatibility use the research as health care product increasingly abundanter, Relevant report emerges in an endless stream, and is also enlivened on health-product market as donkey-hide gelatin, life one, heart source element, Onlly are more Large quantities of traditional Chinese medicine health care products such as nation；But existing health products generally existing is formulated inadequate science, drug effect is relatively low, Healthcare function is not complete, the defect such as security is bad, fails to meet consumer demand.Patent CN201310179375.4, discloses a kind of preparation method of Processed Product of Rhizoma Gastrodiae, with can strengthen immunity of organism work( Energy, liver protection, the function of diuresis；Patent CN201410397218.5 discloses a kind of healthcare function tea and its system Preparation Method.The tea is in addition to including the Radix Astragali, rhizoma Gastrodiae, jujube, Radix Angelicae Sinensis, mulberry fruit, in addition to other 36 tastes Chinese medicine, can Yin Yang balancing, the smooth qi and blood of tune；Energy decline, stress ability decline and immunity can be effectively improved low Inferior subhealth symptom；But above-mentioned patent, compared with the present invention, Chinese medicine preparation of the present invention is more reasonable, preparation More science, curative effect is more notable.

It there is no at present using rhizoma Gastrodiae, Radix Angelicae Sinensis, mulberry fruit, jujube, the Radix Astragali as prescription and this traditional Chinese medicine of the five flavours reasonable compatibility obtained The tcm product obtained.The present inventor studies by substantial amounts of prescription, is tested by substantial amounts of screening, finds With above-mentioned traditional Chinese medicine of the five flavours combination and compatibility to strengthen immunity, antifatigue, raising body hypoxia-bearing, hypoglycemic, drop Blood fat, spot-removing beautifying face beauty, useful for sleeping, Anshen Bunao, Johnson ＆ Johnson are healthy and strong with significant curative effect, more existing Technology and above-mentioned simple, its drug action highly significant, its health care effect are splendid.

The content of the invention

The purpose of the present invention is to be to provide a kind of Chinese medicine composition for health care, and said composition has energy Strengthen immunity, it is antifatigue, improve body hypoxia-bearing, hypoglycemic, reducing blood pressure and blood fat, spot-removing beautifying face beauty, Improve memory, useful for sleeping, Anshen Bunao, Johnson ＆ Johnson's body-building functions.

Another object of the present invention is the preparation side for being to provide a kind of Chinese medicine composition for health care Method.

The Chinese medicine composition of the present invention is made up of rhizoma Gastrodiae, Radix Angelicae Sinensis, mulberry fruit, jujube and the Radix Astragali, in parts by weight Calculate, Chinese medicine composition of the present invention is by weight ratio：10-130 parts of rhizoma Gastrodiae, Radix Angelicae Sinensis 10-130 Part, 20-200 parts of mulberry fruit, 10-130 parts of jujube, 20-200 parts of the Radix Astragali.

It is preferred that proportioning be：20-60 parts of rhizoma Gastrodiae, 20-60 parts of Radix Angelicae Sinensis, 40-80 parts of mulberry fruit, jujube 20-60 Part, 40-80 parts of the Radix Astragali.

More preferably proportioning is：40 parts of rhizoma Gastrodiae, 40 parts of Radix Angelicae Sinensis, 60 parts of mulberry fruit, 40 parts of jujube, the Radix Astragali 60 parts.

Composition of the present invention is directly to pulverize to mix or routinely carry by this area in application Take the active component that technology is extracted to be combined with pharmaceutically acceptable pharmaceutic adjuvant and various pharmaceutical dosage forms are made.

Described excipient substance can for sweetener, preservative, wetting agent, lubricant, wetting agent, disintegrant, Diluent and adhesive.

Present invention also offers a kind of preparation method of the Chinese medicine composition for health care, comprise the following steps： By 10-130 parts of rhizoma Gastrodiae, 10-130 parts of Radix Angelicae Sinensis, 20-200 parts of mulberry fruit, 10-130 parts of jujube, Radix Astragali 20-200 The prepared slices of Chinese crude drugs of part are decocted twice through water extraction, and the water of total 4-12 times of medicinal material amount is added for the first time, decoct 0.5-3 Hour, 180-220 mesh filtering reserved filtrate is standby, and the 4-12 times of decocting measured of second of addition is boiled 0.5-3 hours, 180-220 mesh is filtered, and merges filtrate twice, and it is 1.10~1.15 to take said extracted liquid to be concentrated under reduced pressure into relative density (60 DEG C), obtain concentrate；Various medicine agent are made with pharmaceutically acceptable pharmaceutic adjuvant in concentrate again Type.

It is preferred that preparation method be：By 40 parts of rhizoma Gastrodiae, 40 parts of Radix Angelicae Sinensis, 60 parts of mulberry fruit, 40 parts of jujube is yellow The prepared slices of Chinese crude drugs that 60 parts of stilbene are decocted twice through water extraction, and the water of total 8 times of medicinal material amount is added for the first time, decoct 1.5 Hour, 200 mesh filtering reserved filtrate is standby, and the decoctings of second of addition, 8 times of amounts are boiled 1.5 hours, 200 mesh mistakes Filter, merges filtrate twice, takes said extracted liquid to be concentrated under reduced pressure into relative density for 1.10~1.15 (60 DEG C), Obtain concentrate；Various pharmaceutical dosage forms are made with pharmaceutically acceptable pharmaceutic adjuvant in concentrate again.

Described pharmaceutical dosage form can be oral liquid, tablet, capsule, granule and pill.

When oral liquid is made in Chinese medicine composition of the present invention, comprise the following steps：By above-mentioned concentrate, 0.1-0.3 parts of steviosides, 0.7-0.9 parts of citric acids, 0.3-0.5 parts of potassium sorbates stir, and use purified water 2000ml is settled to, to uniform.Refrigeration stands 24 hours, filtering, takes filtrate.Filtrate is filling, and sterilizing is .

When oral liquid is made in Chinese medicine composition of the present invention, step preferably includes：By above-mentioned concentrate, 0.2 part of stevioside, 0.8 part of citric acid, 0.4 part of potassium sorbate are stirred, and 2000ml is settled to purified water, To uniform.Refrigeration stands 24 hours, filtering, takes filtrate.Filtrate is filling, sterilizing, thus obtaining the product.

Each ingredient properties and effect that the present invention is used are as follows：

Rhizoma Gastrodiae：Orchid family rhizoma Gastrodiae platymiscium rhizoma Gastrodiae Gastrodia elata Bl. stem tuber.It is sweet, put down.Return liver warp. Inflammation Zhijing, Pinggan Yang, dispelling wind and removing obstruction in the meridians.Anxious chronic infantile convulsion is cured mainly, tic contraction, lockjaw, dizziness is had a headache, Hemiplegia, limb fiber crops, arthralgia pain due to rheumatism.Contain phenols, fatty acid and carbohydrate in rhizoma Gastrodiae, with calm, town Bitterly, the pharmacological activity such as anti-epileptic, anti-inflammatory, strengthen immunity, anti-aging, improvement memory.

Radix Angelicae Sinensis：Umbelliferae archangel Radix Angelicae Sinensis Angelica sinensis (Oliv.) Diels [A.polymorpha Maxim.var.sinensis Oliv.] root.Sweet, pungent, hardship, temperature.Return liver, the heart, the spleen channel.Replenishing and activating blood, Meridian and relieving pain, moisturize laxation.The all diseases of the deficiency of blood are cured mainly, irregular menstruation, amenorrhoea is closed, dysmenorrhoea , wei lumps in the abdomen knot gathers, uterine bleeding, Asthenia cold abdominalgia, Impotence numbness, numbness, intestines are dry and shit is difficult, weight, ulcer sores, injury from falling down after dysentery characterized by blood in the stool.Radix Angelicae Sinensis In contain volatile oil and organic acid isoreactivity composition.

Mulberry fruit：Moraceae Mulberry plant mulberry Morus alba L. drying fruit ear.Sweet, acid, trembles with fear.Return liver and kidney channel. Nourishing yin and nourishing blood, promotes the production of body fluid, ease constipation.Cure mainly kidney deficiency and liver and the deficiency of blood essence lose have a dizzy spell, tinnitus, poliosis, Insomnia, quenches one's thirst, the soreness of waist, dry constipation of intestines, the favus of the scalp.With strengthen immunity, promote the medicines such as body hematopoiesis function Reason activity.

Jujube：Rhamnaceae jujube jujube Ziziphus jujuba Mill. fruit.It is sweet, temperature.The thoughts of returning home, spleen, Stomach.Tonifying spleen and stomach, replenishing qi and blood, tranquilizing mind adjusts battalion to defend, and the property of medicine.Weakness of the spleen and the stomach, insufficiency of vital energy and blood is cured mainly, Anorexia and loose stool, lassitude hypodynamia, palpitation and insomnia, hysteria of womam is disharmony between ying and wei.Contain alkaloid, sugar in jujube Glycosides compound, has CNS inhibition, enhancing muscular strength, the medicine such as excited, anti-oxidant, anti-aging, antitumor is immunized Reason activity.

The Radix Astragali：Pulse family Astragalus astragalus mongolicus Astragalus membranaceus (Fisch.) Bge.var.mo- Ngholocus (Bge.) or Astragalus membranacus dry root.It is sweet, temperature.Return lung, the spleen channel.Benefiting qi and raising yang, Gu table stops Sweat, inducing diuresis for removing edema, promoting pus discharge and tissue regeneration.The card of all Qi and blood deficiencies, such as splenasthenic diarrhea are cured mainly, cough due to deficiency of the lung takes off Anus, metroptosis, spontaneous perspiration, night sweat, oedema, blood-arthralgia, ulcer is difficult to burst or burst for a long time and does not holds back.

Chinese medicine composition active component of the present invention is made up of rhizoma Gastrodiae, Radix Angelicae Sinensis, mulberry fruit, jujube, the Radix Astragali, rhizoma Gastrodiae： Suppressing the hyperactive liver to relieve the wind syndrome stops convulsion, Radix Angelicae Sinensis：Replenishing and activating blood, mulberry fruit：Nourishing yin and nourishing blood, jujube：Tonifying spleen and stomach, replenishing qi and blood, peace The mind, the Radix Astragali：Benefiting qi and raising yang, strengthening exterior and reducing sweat.The active component of each Chinese medicine mutually acts synergistically.The present inventor By substantial amounts of prescription study, by substantial amounts of screening test, finally found that with rhizoma Gastrodiae, Radix Angelicae Sinensis, mulberry fruit, Jujube, the Radix Astragali as prescription, and according to screening dosage under, to strengthen immunity, it is antifatigue, improve body it is resistance to Anoxic, hypoglycemic, reducing blood pressure and blood fat, spot-removing beautifying face beauty, raising memory, useful for sleeping, benefit of calming the nerves The effects such as brain, Johnson ＆ Johnson are healthy and strong has significant effect, more existing to treatment inferior health relevant disease determined curative effect Technology and above-mentioned simple, its drug action highly significant, its health care effect are splendid.

The present invention has also listed part test example, it is intended to say to preferably illustrate the beneficial effect of innovation and creation Bright technique effect of the invention, never limits the scope of the present invention.

First, pharmacodynamic study

Test example 1：The Chinese medicine composition strengthen immunity experimental study of the present invention

1st, material

1.1 medicine

Test group 1：Simple is administered：Rhizoma Gastrodiae, mouse stomach administration；

Test group 2：Simple is administered：Radix Angelicae Sinensis, mouse stomach administration；

Test group 3：Simple is administered：Mulberry fruit, mouse stomach administration；

Test group 4：Simple is administered：Jujube, mouse stomach administration；

Test group 5：Simple is administered：The Radix Astragali, mouse stomach administration；

Test group 6：It is derived from oral liquid made from embodiment 1, mouse stomach administration；

Test group 7：It is derived from oral liquid made from embodiment 2, mouse stomach administration；

Test group 8：It is derived from oral liquid made from embodiment 3, mouse stomach administration；

Test group 9：It is derived from oral liquid made from embodiment 4, mouse stomach administration；

Test group 10：It is derived from oral liquid made from embodiment 5, mouse stomach administration；

Test group 11：It is derived from oral liquid made from embodiment 6, mouse stomach administration；

Test group 12：It is derived from oral liquid made from embodiment 7, mouse stomach administration；

Positive controls：Si Bili^RGinseng gastrodia royal jelly oral liquid, mouse stomach administration；

Blank control group：Distilled water, mouse stomach administration.

1.2 animal：Healthy female ICR mouse, body weight 18-22g, mouse original body mass is equal between testing each group Weighing apparatus.

2nd, experimental method

Healthy mice is selected, each Testing index sets mouse 168, be divided into 14 groups, every group 12.Each group is small Mouse is all pressed after the continuous oral gavage of Isodose, the isometric distilled water of blank control group gavage, 30d, surveys every Immune indexes.

2.1 spleens/body weight ratio and antibody-producting cell detection experiment

Mice organs/body weight ratios affect experiment：Mice organs put to death animal after injecting SRBC, 5d, take chest Gland, spleen are weighed, and calculate internal organs/body weight ratio.Splenocyte suspension is prepared simultaneously, carries out antibody-producting cell Determine.

Mouse antibodies cellulation test experience：Spleen is taken, cell suspension is made.Top layer culture medium is dissolved by heating Mixed afterwards with the double Hanks liquid of equivalent, dispense small test tube, every pipe 0.5ml, then into pipe plus 50 μ L 10%SRBC (v/v is prepared with SA liquid), 20 μ L splenocyte suspensions, after rapid mixing, are poured into brush fine jade On the slide of lipolysaccharide thin layer, after after agar solidification, fragmentation level button is placed in glass frame, CO is put into₂Training Support case and incubate 1.5h, the complement (1 then diluted with SA liquid：8) it is added in glass frame groove, continues to incubate After 1.5h, hemolysis plaque number is counted.

2.2 mouse spleen lymphocyte transformation experiments

It is sterile to take spleen, splenocyte suspension is prepared, is washed with Hanks liquid 2 times, 10min (1000r/min) is centrifuged every time, Cell is suspended in 1mL RPMI1640 complete culture solutions, adjustment cell concentration is 3 × 106/mL. Cell suspension point holes is added in 24 well culture plates, per hole 1mL, a hole adds 75 μ L ConA liquid, 5%CO is put as control in another hole₂, cultivate 72h in 37 DEG C of incubators.Culture terminates preceding 4h, is inhaled per hole Supernatant 0.7mL is taken, the RPMI1640 complete culture solutions that 0.7mL is free of calf serum are added, while plus Enter MTT50 μ L, continue to cultivate 4h.After culture terminates, 1mL acid isopropyl alcohol is added per hole, piping and druming is equal It is even, purple crystal is completely dissolved, OD values are determined at 570nm wavelength, to add the OD values in ConA holes Subtract the OD values expression lymphopoiesis ability for being not added with ConA holes.

2.3 mouse delayed allergies are tested

4d measures left back vola pedis thickness after mouse peritoneal injection 2% (v/v) SRBC, sensitization, then in measuring point 20% (v/v) SRBC is subcutaneously injected, 20 μ L are injected per mouse, 24h measures left back foot plantar thickness after injection, together One position is measured 3 times, takes average.DTH is represented with vola pedis thickness difference (swelling degree of the paw) before and after attacking Degree.

2.4 mouse antibodies cellulation test experiences

Spleen is taken, cell suspension is made.Mixed after top layer culture medium is dissolved by heating with the double Hanks liquid of equivalent, Dispense small test tube, every pipe 0.5ml, then into pipe plus 50 μ L 10%SRBC (v/v is prepared with SA liquid), 20 μ L Splenocyte suspension, after rapid mixing, is poured on the slide of brush agarose thin layer, after after agar solidification, incites somebody to action Fragmentation level button is placed in glass frame, is put into CO₂Incubator incubates 1.5h, the complement then diluted with SA liquid (1：8) it is added in glass frame groove, continues to incubate after 1.5h, count hemolysis plaque number.

2.5 mice serum blood lysin test experiences

Sheep blood is taken, brine 3 times centrifuges (2000r/min) 10min, every mouse is through intraperitoneal injection every time 2% hematocrit SRBC0.2mL is immunized.After 4d, extract eyeball and take blood in centrifuge tube, place about 1h, Solidification blood and tube wall are peeled off, serum is fully analyzed, 2000r/min centrifugation 10min collect serum. With SA buffer solutions by serum-dilution.Serum 1mL after dilution is put in test tube, 10% (V/V) is sequentially added SRBC0.5mL, complement 1mL.The another control tube for setting not increase serum (with the replacement of SA buffer solutions).Put 37 DEG C It is incubated in water bath with thermostatic control after 25min, ice bath terminating reaction.2000r/min centrifuges 10min, takes supernatant 1mL, Plus Dou Shi reagents 1mL.10% (V/V) SRBC0.25mL is taken simultaneously, plus Dou Shi reagents are to 4mL, it is fully mixed It is even, place after 10min, sentence control tube in 540nm and make blank, each pipe OD value is surveyed respectively.Haemolysis The amount of element is with half hemolytic value (HC₅₀) represent.

2.6 mouse carbonic clearances are tested

To mouse tail vein injection 1：The india ink of 3 dilutions, treats that prepared Chinese ink is injected after timing immediately, injection prepared Chinese ink 10min, takes the μ L of blood 20, and be added into 2mL Na from angular vein clump respectively₂CO₃In solution, with purple Outer visible spectrophotometer is at 600nm wavelength with Na₂CO₃Solution makees blank control densitometric value (OD). Mouse is put to death, takes liver and spleen to weigh, phagocytic index is calculated.

2.7 NK cells in mice determinations of activity

24h washes target cell Secondary Culture 3 times using preceding with Hanks liquid before experiment, complete with RPMI1640 Full nutrient solution adjustment cell concentration is 4 × 105/mL.Mouse cervical dislocation is put to death, sterile to take spleen, preparation Splenocyte suspension, is washed 2 times with Hanks liquid, 10min (1000r/min) is centrifuged every time.Supernatant is abandoned by cytoplasm Upspring, 2 times of Hanks liquid of 0.5mL and 8mL are added after adding 0.5mL aqua sterilisa splitting erythrocytes, 20s Hanks liquid, centrifugation 10min (1000r/min) is trained completely with RPMI1640 of the 1mL containing 10% calf serum Nutrient solution is resuspended, and is counted after being diluted with 1% glacial acetic acid, and adjustment cell concentration is 2 × 107/mL.By target cell 96 well culture plates are added, per the μ L of hole 100, test hole adds 100 μ L splenocytes, and (effect target compares 50：1), certainly Right release aperture adds 100 μ L nutrient solutions, and maximum release aperture adds 100 μ L 1%NP40,37 DEG C of culture 4h, Centrifugation, takes the μ L of supernatant 100 to put in 96 hole elisa Plates, adds the μ L of LDH matrix liquids 100, reacts 6min, With 1mol/L HCL terminating reactions, the measured value OD values at ELIASA 490nm.

3rd, experimental result

3.1 changes of weight situations

Before being tested, all mouse weigh body weight value；Mouse receives after tested material 30 days, and all mouse are again It is secondary to weigh body weight value, because mouse original body mass is balanced, receives body weight after tested material and, also without significant difference, test Group 1-12 and positive controls are compared with blank control group, P>0.05, thus invention formulation to mouse weight without Influence.

3.2 immunity measurement results

According to《Health-care food is evaluated and technical specification》Experiment acquired results, are now divided into by relevant criterion：It is dirty Device/body weight ratio measurement, comprising spleen/body weight ratio, thymus gland/body weight ratio, the results are shown in Table 1；Cellular immunity Functional examination, is tested, to mouse delayed allergy experiment comprising influence is converted on mouse spleen lymphocyte, It the results are shown in Table 2；Humoral immune function is determined, and includes mouse antibodies cellulation test experience, mice serum blood Lysin test experience, the results are shown in Table 3；Monocytes/macrophages functional examination, comprising on the influence examination of mouse carbonic clearance Test, Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell is tested, and the results are shown in Table 4；NK cytoactive detections, knot Fruit is shown in Table 5.

Influence of each group of table 1 to mice organs/body weight ratio

Group	Spleen/body weight ratio (× 10²)	Thymus gland/body weight ratio (× 10²)
			Test group 1	0.40±0.08	0.33±0.03
Test group 2	0.46±0.06	0.33±0.04
			Test group 3	0.42±0.03	0.34±0.03
Test group 4	0.40±0.01	0.31±0.04
			Test group 5	0.42±0.04	0.36±0.05
Test group 6	0.41±0.07	0.34±0.02
			Test group 7	0.43±0.05	0.32±0.06
Test group 8	0.42±0.01	0.31±0.03
			Test group 9	0.40±0.06	0.33±0.06
Test group 10	0.43±0.04	0.33±0.04
			Test group 11	0.42±0.02	0.32±0.06
Test group 12	0.43±0.03	0.31±0.03
			Positive controls	0.49±0.06	0.38±0.04
Blank control group	0.41±0.02	0.33±0.05

As a result：Influence of each group to mouse spleen/body weight ratio：Each test group and positive controls and blank pair Compare according to group, P>0.05；Influence of each group to mouse thymus/body weight ratio：Test group 1-12 and positive control Group is compared with blank control group, P>0.05；Conclusion：Test group 1-12 and positive controls pharmaceutical composition On mice organs/body weight ratio without influence.

The each group of table 2 is to mouse cell immunity function result

Group	Lymphocyte proliferation ability (OD differences)	Swelling degree of the paw
			Test group 1	0.36±0.05	0.42mm±0.03mm
Test group 2	0.28±0.07	0.40mm±0.06mm
			Test group 3	0.32±0.06	0.38mm±0.06mm
Test group 4	0.26±0.04	0.37mm±0.05mm
			Test group 5	0.30±0.08	0.39mm±0.07mm
Test group 6	0.46±0.07	0.48mm±0.05mm
			Test group 7	0.52±0.09	0.56mm±0.08mm
Test group 8	0.58±0.06	0.62mm±0.04mm
			Test group 9	0.67±0.04	0.69mm±0.05mm
Test group 10	0.69±0.06	0.70mm±0.08mm
			Test group 11	0.70±0.05	0.71mm±0.07mm
Test group 12	0.70±0.09	0.71mm±0.09mm
			Positive controls	0.43±0.04	0.43mm±0.07mm
Blank control group	0.10±0.03	0.31mm±0.03mm

Remarks：Lymphocyte proliferation ability：Test group 1-12 and positive controls are compared with blank control group, P ＜ 0.05；Swelling degree of the paw：Test group 1-12 and positive controls are compared with blank control group, P ＜ 0.05；

Conclusion：This experiment each group and positive controls are positive to mouse cell immunity test result, test group 6-12 effects are better than positive controls, test group 1-5 simples administration group, low component formula group 6, the positive Control group effect is undesirable, and significantly, test group 9-11 effects amplification is slow, test group for test group 7-12 effects 11 compared with high component proportion group 12, and difference on effect is not obvious, therefore preferred composition of the present invention proportioning is： 10-130 parts of rhizoma Gastrodiae, 10-130 parts of Radix Angelicae Sinensis, 20-200 parts of mulberry fruit, 10-130 parts of jujube, Radix Astragali 20-200 Part；Using the amount ranges, worked well in terms of mouse cell immunologic function is strengthened, wherein best composition is Test group 9.

The each group of table 3 is to mouse humoral immune function result

Remarks：Hemolysis plaque number：Test group 1-12 of the present invention is compared with blank control group, P ＜ 0.05；

Half hemolytic value：Test group 1-12 of the present invention is compared with blank control group, P ＜ 0.05.

Conclusion：This experiment each group is positive to mouse humoral immune function test result, and test group 6-12 effects will It is better than positive controls, test group 1-5 simples administration group, low component formula group 6, positive controls effect Undesirable, significantly, test group 9-11 effects amplification is slow for test group 7-12 effects, test group 11 with high group Distribution ratio group 12 is compared, and difference on effect is not obvious, therefore preferred composition of the present invention proportioning is：Rhizoma Gastrodiae 10-130 Part, 10-130 parts of Radix Angelicae Sinensis, 20-200 parts of mulberry fruit, 10-130 parts of jujube, 20-200 parts of the Radix Astragali；Using this Amount ranges, work well in enhancing mouse humoral immune function aspects, and wherein best composition is test group 9.

The each group of table 4 influences result to mouse monokaryon-macrophage function

Remarks：Carbonic clearance ability phagocytic index：Test group 1-12 of the present invention is compared with blank control group, P ＜ 0.05；Half hemolytic value：Swallow chicken red blood cell ability phagocytic percentage and phagocytic index test group 1-12 and sky White control group compares, P ＜ 0.05；

Conclusion：This experiment each group is positive to mouse monokaryon-macrophage function result of the test, test group 6-12 Effect is better than positive controls, test group 1-5 simples administration group, low component formula group 6, positive control Group effect is undesirable, and significantly, test group 9-11 effects amplification is slow, test group 11 for test group 7-12 effects Compared with high component proportion group 12, difference on effect is not obvious, therefore preferred composition of the present invention proportioning is：My god It is numb 10-130 parts, 10-130 parts of Radix Angelicae Sinensis, 20-200 parts of mulberry fruit, 10-130 parts of jujube, Radix Astragali 20-200 Part；Using the amount ranges, worked well in terms of mouse monokaryon-macrophage function is strengthened, wherein most preferably Component is test group 9.

Influence result of each group of table 5 to NK cells in mice activity

Group	NK cytoactives (%)
		Test group 1	54.3±10.2
Test group 2	50.6±9.8
		Test group 3	50.4±9.4
Test group 4	52.7±11.0
		Test group 5	53.4±11.2
Test group 6	63.4±11.5
		Test group 7	68.9±12.1
Test group 8	75.2±12.3
		Test group 9	80.8±13.2
Test group 10	82.1±13.5
		Test group 11	83.3±14.3
Test group 12	83.2±11.9
		Positive controls	58.3±13.9
Blank control group	45.7±9.6

As a result：Test group 1-12 of the present invention is compared with blank control group, P ＜ 0.05；

Conclusion：This experiment each group is positive on Mice Mice NK cytoactives influence result of the test, test group 6-12 Effect is better than positive controls, test group 1-5 simples administration group, low component formula group 6, positive control Group effect is undesirable, and significantly, test group 9-11 effects amplification is slow, test group 11 for test group 7-12 effects Compared with high component proportion group 12, difference on effect is not obvious, therefore preferred composition of the present invention proportioning is：My god It is numb 10-130 parts, 10-130 parts of Radix Angelicae Sinensis, 20-200 parts of mulberry fruit, 10-130 parts of jujube, Radix Astragali 20-200 Part；Using the amount ranges, worked well in terms of NK cells in mice activity is strengthened, wherein best composition is Test group 9.

It is overall test result indicates that, the spleen lymph that preferred compound composition of the present invention can improve mouse is thin Born of the same parents' multiplication capacity, increase antibody-producting cell number, raising serum hemolysin, enhancing mouse peritoneal macrophage are thin Endocytosis is bitten chicken red blood cell ability, enhancing mouse monokaryon-macrophage phagocytic function, enhancing NK cells in mice and lived Property；On spleen/body weight ratio, thymus gland/body weight ratio, swelling degree of the paw without influence；Illustrate of the invention effective The action effect of compound composition strengthen immunity is notable.

Test example 2：Present composition enhancing mouse anti-reflecting fatigue effect experimental study

1st, material

1.1 medicine：Test group 1-7：It is derived from embodiment 1-7 and matches obtained oral liquid by different prescriptions；

Positive controls：American ginseng beverage；

Blank control group：Distilled water；

1.2 animal：Kunming mouse 108, body weight 18-22g is randomly divided into nine groups.

2nd, experimental method

Mouse is given after medicine 30min, and the mouse of the body weight sheet lead of root of the tail portion load 5% is placed in into swimming trunk middle reaches Swimming, depth of water 40cm, 25 DEG C of water temperature, observation mouse, which is put into, to be sink to the water-bed time floated powerless again to hold in water Continuous swimming time.

3rd, experimental result

The composition of the present invention, which can be obviously prolonged mouse and bear a heavy burden, continues swimming time, and concrete outcome is shown in Table 6.

Table 6：Influence of the present composition to mouse swimming time：

Sequence number	Group	Dosage (ml)	Swimming with a load attached to the body mean survival time (min)
				1	Embodiment 1	50	10.1
2	Embodiment 2	50	12.3
				3	Embodiment 3	50	14.5
4	Embodiment 4	50	17.0
				5	Embodiment 5	50	17.3
6	Embodiment 6	50	17.5
				7	Embodiment 7	50	17.5
8	Positive controls	50	11.0
				9	Blank control group	50	6.9

1-7 of the embodiment of the present invention, positive controls are compared with blank control group, P ＜ 0.05；

Result of the test shows：The composite preparation and positive controls of the present invention can significantly extend mouse heavy burden and continue Swimming time, embodiment 2-7 effects are more notable compared with positive controls, and embodiment 1 is low component formula group, Effect is undesirable, and embodiment 9-11 extension mouse, which bear a heavy burden, to continue swimming time and slowly increase, embodiment 11 with it is real Example 12 is applied compared to the lasting swimming time of mouse heavy burden no longer to increase.In summary, preferred composition of the present invention is matched somebody with somebody Than for：10-130 parts of rhizoma Gastrodiae, 10-130 parts of Radix Angelicae Sinensis, 20-200 parts of mulberry fruit, 10-130 parts of jujube, the Radix Astragali 20-200 parts；In this amount ranges, present composition preparation is to the non-convention of mouse anti-reflecting fatigue ability effect Think, wherein optimum formula is 40 parts of rhizoma Gastrodiae, 40 parts of Radix Angelicae Sinensis, 60 parts of mulberry fruit, 40 parts of jujube, the Radix Astragali 60 Part.

Test example 3：The present composition improves body hypoxia-bearing effect experimental study

1st, material

Positive controls：Squalene soft capsules；

Blank control group：Distilled water；

2nd, experimental method

Lh after administrating, takes mouse by being only put into 250m] closed in wide-mouth bottle (soda lime 20g, bottleneck are put in bottle Apply vaseline), observe each group mouse diing time.(to breathe stopping) is the time-to-live.

3rd, result

Table 7：The effect of invention formulation confrontation anoxia in mice (N=12)

Sequence number	Group	Dosage (ml)	The normobaric hypoxia time-to-live (min)
				1	Embodiment 1	50	22.4
2	Embodiment 2	50	26.8
				3	Embodiment 3	50	30.5
4	Embodiment 4	50	35.1
				5	Embodiment 5	50	35.7
6	Embodiment 6	50	36.0
				7	Embodiment 7	50	35.9
8	Positive controls	50	11.0
				9	Blank control group	50	10.3

Result of the test shows：The composite preparation and positive controls of the present invention can significantly extend mouse normobaric hypoxia Time-to-live, embodiment 2-7 effects are more notable compared with positive controls, and embodiment 1 is low component formula group, Effect is undesirable, and the embodiment 4-6 extension mouse normobaric hypoxia time-to-live slowly increases, and embodiment 6 is with implementing Example 7 no longer increases compared to the mouse normobaric hypoxia time-to-live.In summary, preferred composition proportioning of the present invention is： 10-130 parts of rhizoma Gastrodiae, 10-130 parts of Radix Angelicae Sinensis, 20-200 parts of mulberry fruit, 10-130 parts of jujube, Radix Astragali 20-200 Part；In this amount ranges, present composition preparation is ideal to mouse hypoxia-bearing capability effect, wherein Optimum formula is 40 parts of rhizoma Gastrodiae, 40 parts of Radix Angelicae Sinensis, 60 parts of mulberry fruit, 40 parts of jujube, 60 parts of the Radix Astragali.

Test example 4：Present composition hypoglycemic effect experimental study

1st, material

Positive controls：Ginseng；

Blank control group：Distilled water；

1.2 animal：Male Kunming strain mice 120, body weight 18-22g is randomly divided into 10 groups.

2nd, experimental method

2.1 alloxan diabetes mouse models

Mouse 60 is injected intraperitoneally only with 1% alloxan normal saline solution 320mg/kg, 1 time a day, Common 2d.With blood glucose rise greater than, equal to more than 50% before injection to replicate successfully.

2.2 blood sugar detection

Mouse gavaging is administered for three days on end, is punctured on an empty stomach from mouse orbit veniplex during the 4th day morning 8 and takes blood, Determined with enzymatic measurement.

3rd, experimental result

Table 8：Present composition preparation to hypoglycemic effect (N=12)

Result of the test shows：The high blood of alloxan can significantly drop in the composite preparation and positive controls of the present invention Sugar, embodiment 2-7 effects are more notable compared with positive controls, and embodiment 1 is low component formula group, and effect is not Ideal, embodiment 4-6 drop alloxan hyperglycemias slowly increase, and embodiment 6 is compared with Example 7 Drop alloxan hyperglycemia no longer increases.In addition, euglycemia mouse is administered embodiment 6, gavage There was no significant difference for front and rear fasting blood-glucose, illustrates invention formulation to normal mouse without reduction blood glucose effect.To sum up Described, preferred composition proportioning of the present invention is：10-130 parts of rhizoma Gastrodiae, 10-130 parts of Radix Angelicae Sinensis, mulberry fruit 20-200 Part, 10-130 parts of jujube, 20-200 parts of the Radix Astragali；In this amount ranges, present composition preparation is to small Alloxan hyperglycemia effect drops ideal, wherein optimum formula is 40 parts of rhizoma Gastrodiae, Radix Angelicae Sinensis 40 Part, 60 parts of mulberry fruit, 40 parts of jujube, 60 parts of the Radix Astragali.

Test example 5：Present composition reducing blood pressure and blood fat acts on experimental study

Patient 50 after present invention selection clinical definite, the example of formulations 2 prepared with the present invention, implementation Example 6 carries out treatment hypertension, hyperlipemia relevant disease and carries out analysis and investigation, and its result see the table below 9：

Table 9：Investigation of the present composition preparation to treatment high pressure hyperlipidaemic conditions：

Investigation project	The preparation that the present composition is made
		Clinical practice is evaluated	Total effective rate 90.4%
Clinical adverse is evaluated	Obvious adverse reaction is not found

Result of the test explanation：The present composition is diseases related to treatment hypertension, hyperlipemia, curative effect highly significant.

Test example 6：Present composition eliminating chloasma, pigmentation effect effect experimental study

1st, material：

1.1 medicine：

Invention formulation：According to preparation produced by the present invention, embodiment 2, embodiment 6.

Compare medicine：HUAYU QUBAN JIAONANG (Shanxi Ren Yuan Tang Pharmaceutical Co., Ltd.'s production), specification：0.32g/.

1.2 subjects：Women, 18~50 years old age, Head And Face visually observes different yellowish-brown of visible level 80, spot, without symptoms such as any sufferings.

2nd, test method

2.1 test method

80 patient with chloasma are randomly divided into four groups, treatment group 1 (embodiment 2), treatment group 2 (implement Example 6), control group and blank control group, every group 20, treatment group 1：18~46 years old age, average age 35.67 years old, average course of disease 7.89；Treatment group 2：It is 20~45 years old age, average age year 36.83, average The course of disease 6.95；Control group：21~48 years old age, average age 37.31 years old, average course of disease 7.03 years； Blank control group：20~50 years old age, average age 37.52 years old, average course of disease 7.42, four groups of subjects Basic condition, age, the course of disease there are no significant difference, four groups of data have comparativity.

The preparation (embodiment 2, embodiment 6) that treatment group is made using the present invention, orally, 1 times a day, often Secondary 50ml, continuously takes 90 days, and subject stops using other nti-freckle products during taking medicine.

Control group using control medicine, orally, 2 times a day, one time 5, continuously take 90 days.

Blank control group is without using any medicine for removing speckle, and three groups of diet is identical.

2.2 experimental observation indexs

2.2.1 the size of patch before and after observation is treated, the change of color spot shade

With length and width of the same chloasma of tape measure before and after tested, color spot area is calculated；According to Chinese section Institute's Institute of Geography development and design, what Mapping Press published for 1992《Practical standard colour atla》(first edition) In brown (Y+M+BK is yellow+pinkish red+black folded color) colour atla be the chloasma depth criterion：Ⅰ (15,20,5), II (30,40,10), III (40,60,15)

Freckle effect judges

(1) it is effective：Chloasma color is substantially shoaled, and area is reduced>1/3, new chloasma is not produced；

(2) effectively：Chloasma lighter, area reduces≤1/3, and new chloasma is not produced；

(3) it is invalid：Chloasma color and area are without significant change.

2.2.2 curative effect index and adverse reaction before and after observation treatment, and detect activity of SOD in serum and MDA contents.SOD is determined using yellow crash cry of certain animals enzymatic measurement, and MDA is determined and used thiobarbituricacidα- method, south Bioengineering Research Institute's preparation is built up in capital

3rd, result of the test

1.2.3.1 freckle effect result of the test (being shown in Table 10)

10 two groups of freckle effects of table compare

* P is compared with blank control group<0.01, there is significant difference

* is compared P with blank control group<0.01, there is significant difference

Treatment group P compared with blank group>0.05, there was no significant difference.

As a result show：

After subject continuously takes invention formulation 90 days, the color of the chloasma of Head And Face substantially shoal until Disappear, color spot area is reduced significantly, and 85.0% subject's color spot area is reduced beyond the 1/3 of primary colors spot area More than, wherein the chloasma of 50% subject (i.e. 10 subjects) is wholly absent, and do not observe new Chloasma occur, total effective rate of the invention reaches 95.0%, and the colour of skin exquisiteness of subject is pale, illustrate The spot-eliminating composition of invention has effects that spot-eliminating beauty treatment.

It can be seen that from data above, the freckle effect for the treatment of group is suitable with control group.

The preparation of present invention subject during testing, without any allergic reaction and other adverse reactions, illustrates this hair Bright spot-eliminating composition has no adverse effects to human body.

2nd, technical study

Test example 7：Oral liquid formulations sweetener of the present invention, the research of acid choice experiment

Oral liquid of the present invention is made by traditional Chinese medicine extraction, there is heavier Chinese medicine bitter taste, and sweet tea need to be added to improve mouthfeel Taste agent, acid.Stevioside is that one kind essence from catananche's STEVIA REBAUDIANA (or stevia rebaudian leaf) is carried New type natural sweetener, compared with sucrose, the characteristics of it has high sugariness low heat value does not interfere with blood sugar level Or interference insulin, while without any calorie, can be carried to diabetes patient in terms of heat is always taken in budget For more flexible selections, and contribute to control body weight, be particularly suitable for obesity, diabetes, hypertension, small several The condition subjects such as carious tooth eat, safe.Citric acid also known as citric acid, with gentle frank tart flavour, make Food industry is widely used in for acid.

1st, test method：

Oral liquid prescription of the present invention：

Prescription 1：Rhizoma Gastrodiae 10g, Radix Angelicae Sinensis 10g, mulberry fruit 20g, jujube 10g, Radix Astragali 20g；

Prescription 2：Rhizoma Gastrodiae 20g, Radix Angelicae Sinensis 20g, mulberry fruit 40g, jujube 20g, Radix Astragali 40g；

Prescription 3：Rhizoma Gastrodiae 40g, Radix Angelicae Sinensis 40g, mulberry fruit 60g, jujube 40g, Radix Astragali 60g；

Prescription 4：Rhizoma Gastrodiae 60g, Radix Angelicae Sinensis 60g, mulberry fruit 80g, jujube 60g, Radix Astragali 80g；

Prescription 5：Rhizoma Gastrodiae 130g, Radix Angelicae Sinensis 130g, mulberry fruit 200g, jujube 130g, Radix Astragali 200g；

Sweetener, acid selection scheme table

Each prescription adds stevioside by said ratio respectively and oral liquid is made in citric acid, and oral liquid is tasted respectively Mouthfeel.

2nd, result of the test

The oral liquid mouthfeel that each prescription is made by said ratio addition stevioside and citric acid respectively is as follows：

Conclusion：The amount that prescription 1-5 adds stevioside is 0.1-0.3g, and the amount for adding citric acid is 0.7-0.9g When, oral liquid taste of traditional Chinese medicine is obviously improved, sour-sweet moderate, tasty, it is easy to be accepted.Wherein add stevioside The amount of glucoside is 0.2g, when the amount for adding citric acid is 0.8g, best results.

Test example 8：Oral liquid formulations preservative choice experiment research of the present invention

A certain amount of preservative is added in oral liquid can reach antibacterial and corrosion-resistant effect.Potassium sorbate is state The antisepsis antistaling agent of the highly effective and safe of border food and agricultural organization and Health Organisation recommendations, be widely used in food, beverage, The industries such as tobacco, agricultural chemicals, cosmetics.

1st, test method：

Oral liquid prescription of the present invention：

Preservative selection scheme table

Sequence number	Prescription 1	Prescription 2	Prescription 3	Prescription 4	Prescription 5
						1	Potassium sorbate 0.2g	Potassium sorbate 0.2g	Potassium sorbate 0.2g	Potassium sorbate 0.2g	Potassium sorbate 0.2g
2	Potassium sorbate 0.3g	Potassium sorbate 0.3g	Potassium sorbate 0.3g	Potassium sorbate 0.3g	Potassium sorbate 0.3g
						3	Potassium sorbate 0.4g	Potassium sorbate 0.4g	Potassium sorbate 0.4g	Potassium sorbate 0.4g	Potassium sorbate 0.4g
4	Potassium sorbate 0.5g	Potassium sorbate 0.5g	Potassium sorbate 0.5g	Potassium sorbate 0.5g	Potassium sorbate 0.5g
						5	Potassium sorbate 0.6g	Potassium sorbate 0.6g	Potassium sorbate 0.6g	Potassium sorbate 0.6g	Potassium sorbate 0.6g

Each prescription adds potassium sorbate by said ratio respectively and oral liquid is made, and fungistatic effect is detected respectively.

2nd, result of the test

The oral liquid fungistatic effect that each prescription is made by said ratio addition potassium sorbate respectively is as follows：

Sequence number	Prescription 1	Prescription 2	Prescription 3	Prescription 4	Prescription 5
						1	It is unqualified	It is unqualified	It is unqualified	It is unqualified	It is unqualified
2	It is qualified	It is qualified	It is qualified	It is qualified	It is qualified
						3	Well	Well	Well	Well	Well
4	It is qualified	It is qualified	It is qualified	It is qualified	It is qualified
						5	It is unqualified	It is unqualified	It is unqualified	It is unqualified	It is unqualified

Conclusion：When the amount that prescription 1-5 adds potassium sorbate is 0.3-0.5g, oral liquid fungistatic effect can be closed Lattice, reach standard requirement.When the amount for wherein adding potassium sorbate is 0.4g, best results.

Embodiment：

Embodiment 1：The preparation technology (low component) of present composition oral liquid

Prescription：Rhizoma Gastrodiae 5g, Radix Angelicae Sinensis 5g, mulberry fruit 10g, jujube 5g, Radix Astragali 10g；

Above-mentioned five tastes medicine materical crude slice is decocted twice with water extraction, the water of total 3 times of amounts of medicinal material amount is added for the first time, is decocted 0.4 hour, 200 mesh filtering reserved filtrate was standby, and the decoctings of second of addition, 3 times of amounts are boiled 0.4 hour, 200 mesh Filtering, merges filtrate twice, takes said extracted liquid to be concentrated under reduced pressure into relative density for 1.10~1.15 (60 DEG C), Obtain concentrate.Concentrate, 0.09g steviosides, 0.6g citric acids, 0.2g potassium sorbates are stirred, 2000ml is settled to purified water, to uniform.Refrigeration stands 24 hours, filtering, takes filtrate.Filtrate is filling, Sterilizing, thus obtaining the product.

Embodiment 2：The preparation technology of present composition oral liquid

Prescription：Rhizoma Gastrodiae 10g, Radix Angelicae Sinensis 10g, mulberry fruit 20g, jujube 10g, Radix Astragali 20g；

Above-mentioned five tastes medicine materical crude slice is decocted twice with water extraction, the water of total 4 times of medicinal material amount is added for the first time, is decocted 0.5 hour, 200 mesh filtering reserved filtrate was standby, and the decoctings of second of addition, 4 times of amounts are boiled 0.5 hour, 200 mesh Filtering, merges filtrate twice, takes said extracted liquid to be concentrated under reduced pressure into relative density for 1.10~1.15 (60 DEG C), Obtain concentrate.Concentrate, 0.1g steviosides, 0.7g citric acids, 0.3g potassium sorbates are stirred, used Purified water is settled to 2000ml, to uniform.Refrigeration stands 24 hours, filtering, takes filtrate.Filtrate is filling, Sterilizing, thus obtaining the product.

Embodiment 3：The preparation technology of present composition oral liquid

Prescription：Rhizoma Gastrodiae 20g, Radix Angelicae Sinensis 20g, mulberry fruit 40g, jujube 20g, Radix Astragali 40g；

Above-mentioned five tastes medicine materical crude slice is decocted twice with water extraction, the water of total 8 times of medicinal material amount is added for the first time, is decocted 1.5 hours, 200 mesh filtering reserved filtrate was standby, and the decoctings of second of addition, 8 times of amounts are boiled 1.5 hours, 200 mesh Filtering, merges filtrate twice, takes said extracted liquid to be concentrated under reduced pressure into relative density for 1.10~1.15 (60 DEG C), Obtain concentrate.Concentrate, 0.2g steviosides, 0.8g citric acids, 0.4g potassium sorbates are stirred, used Purified water is settled to 2000ml, to uniform.Refrigeration stands 24 hours, filtering, takes filtrate.Filtrate is filling, Sterilizing, thus obtaining the product.

Embodiment 4：The preparation technology of present composition oral liquid

Prescription：Rhizoma Gastrodiae 40g, Radix Angelicae Sinensis 40, mulberry fruit 60g, jujube 40g, Radix Astragali 60g；

Embodiment 5：The preparation technology of present composition oral liquid

Prescription：Rhizoma Gastrodiae 60g, Radix Angelicae Sinensis 60g, mulberry fruit 80g, jujube 60g, Radix Astragali 80g；

Embodiment 6：The preparation technology of present composition oral liquid

Prescription：Rhizoma Gastrodiae 130g, Radix Angelicae Sinensis 130, mulberry fruit 200g, jujube 130g, Radix Astragali 200g；

Above-mentioned five tastes medicine materical crude slice is decocted twice with water extraction, the water of total 12 times of medicinal material amount is added for the first time, is decocted 3 hours, 200 mesh filtering reserved filtrate was standby, and the decoctings of second of addition, 12 times of amounts are boiled 3 hours, 200 mesh mistakes Filter, merges filtrate twice, takes said extracted liquid to be concentrated under reduced pressure into relative density for 1.10~1.15 (60 DEG C), Obtain concentrate.Concentrate, 0.3g steviosides, 0.9g citric acids, 0.5g potassium sorbates are stirred, used Purified water is settled to 2000ml, to uniform.Refrigeration stands 24 hours, filtering, takes filtrate.Filtrate is filling, Sterilizing, thus obtaining the product.

Embodiment 7：The preparation technology (high component) of present composition oral liquid

Prescription：Rhizoma Gastrodiae 140g, Radix Angelicae Sinensis 140g, mulberry fruit 210g, jujube 140g, Radix Astragali 210g；

Above-mentioned five tastes medicine materical crude slice is decocted twice with water extraction, the water of total 13 times of medicinal material amount is added for the first time, is decocted 3.5 hours, 200 mesh filtering reserved filtrate was standby, and 3.5 hours, 200 are boiled in the decoctings of second of addition, 13 times of amounts Mesh is filtered, and merges filtrate twice, takes said extracted liquid to be concentrated under reduced pressure into relative density for 1.10~1.15 (60 DEG C), Obtain concentrate.Concentrate, 0.35g steviosides, 1.0g citric acids, 0.6g potassium sorbates are stirred, 2000ml is settled to purified water, to uniform.Refrigeration stands 24 hours, filtering, takes filtrate.Filtrate is filling, Sterilizing, thus obtaining the product.

Claims

1. a kind of Chinese medicine composition for health care, it is characterised in that be made up of the raw material of following weight parts： 10-130 parts of rhizoma Gastrodiae, 10-130 parts of Radix Angelicae Sinensis, 20-200 parts of mulberry fruit, 10-130 parts of jujube, Radix Astragali 20-200 Part.

2. Chinese medicine composition according to claim 1, it is characterised in that by the raw material group of following weight parts Into：20-60 parts of rhizoma Gastrodiae, 20-60 parts of Radix Angelicae Sinensis, 40-80 parts of mulberry fruit, 20-60 parts of jujube, Radix Astragali 40-80 Part.

3. Chinese medicine composition according to claim 1 or 2, it is characterised in that by the original of following weight parts Material composition：40 parts of rhizoma Gastrodiae, 40 parts of Radix Angelicae Sinensis, 60 parts of mulberry fruit, 40 parts of jujube, 60 parts of the Radix Astragali.

4. according to any Chinese medicine compositions of claim 1-3, it is characterised in that described composition can be straight The active component for mixing or being extracted by this area conventional extraction techniques of pulverizing is connect with can pharmaceutically connect Various pharmaceutical dosage forms are made in the pharmaceutic adjuvant received.

5. excipient substance according to claim 4 can for sweetener, acid, preservative, wetting agent, Lubricant, wetting agent, disintegrant, diluent and adhesive.

6. the preparation method of a kind of Chinese medicine composition for health care, it is characterised in that comprise the following steps：

By 10-130 parts of rhizoma Gastrodiae, 10-130 parts of Radix Angelicae Sinensis, 20-200 parts of mulberry fruit, 10-130 parts of jujube, the Radix Astragali 20-200 parts of the prepared slices of Chinese crude drugs are decocted twice through water extraction, and the water of total 4-12 times of medicinal material amount is added for the first time, are decocted Boil 0.5-3 hours, 180-220 mesh filtering reserved filtrate is standby, 0.5-3 is boiled in the 4-12 times of decocting measured of second of addition Hour, the filtering of 180-220 mesh merges filtrate twice, takes said extracted liquid to be concentrated under reduced pressure into relative density and is 1.10~1.15 (60 DEG C), obtain concentrate；Concentrate is made with pharmaceutically acceptable pharmaceutic adjuvant again Various pharmaceutical dosage forms.

7. preparation method according to claim 6, it is characterised in that comprise the following steps：By rhizoma Gastrodiae 40 parts, 40 parts of Radix Angelicae Sinensis, 60 parts of mulberry fruit, 40 parts of jujube, the prepared slices of Chinese crude drugs that 60 parts of the Radix Astragali are decocted through water extraction Boil twice, the water of total 8 times of medicinal material amount added for the first time, decoct 1.5 hours, 200 mesh filtering reserved filtrate is standby, The decoctings of second of addition, 8 times of amounts are boiled 1.5 hours, and the filtering of 200 mesh merges filtrate twice, takes said extracted Liquid is concentrated under reduced pressure into relative density for 1.10~1.15 (60 DEG C), obtains concentrate；Again by concentrate and pharmaceutically Various pharmaceutical dosage forms are made in acceptable pharmaceutic adjuvant.

8. pharmaceutical dosage form according to claim 6 is：Oral liquid, tablet, capsule, granule and ball Agent.

9. pharmaceutical dosage form according to claim 8, it is characterised in that：When oral liquid is made, including it is following Step：By above-mentioned concentrate, 0.1-0.3 parts of steviosides, 0.7-0.9 parts of citric acids, 0.3-0.5 parts of sorbic acids Potassium is stirred, and 2000ml is settled to purified water, to uniform.Refrigeration stands 24 hours, filtering, takes filter Liquid.Filtrate is filling, sterilizing, thus obtaining the product.

10. formulation according to claim 7, it is characterised in that：When oral liquid is made, comprise the following steps： Above-mentioned concentrate, 0.2 part of stevioside, 0.8 part of citric acid, 0.4 part of potassium sorbate are stirred, with pure Change water and be settled to 2000ml, to uniform.Refrigeration stands 24 hours, filtering, takes filtrate.Filtrate is filling, goes out Bacterium produces.

11. according to a kind of any described Chinese medicine compositions for health care of claim 1-3, its feature It is：There is energy strengthen immunity, antifatigue, raising body hypoxia-bearing, hypoglycemic, hypotensive for preparing Reducing blood lipid, spot-removing beautifying face beauty, raising memory, useful for sleeping, Anshen Bunao, the medicine of Johnson ＆ Johnson's body-building functions Application in product or health products.