CN114767906B - Disinfection device and disinfection method for cell collection and cultivation - Google Patents
Disinfection device and disinfection method for cell collection and cultivation Download PDFInfo
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- CN114767906B CN114767906B CN202210330072.7A CN202210330072A CN114767906B CN 114767906 B CN114767906 B CN 114767906B CN 202210330072 A CN202210330072 A CN 202210330072A CN 114767906 B CN114767906 B CN 114767906B
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- sliding plate
- sealing
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- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 140
- 238000000034 method Methods 0.000 title claims abstract description 24
- 230000001954 sterilising effect Effects 0.000 claims abstract description 63
- 239000007788 liquid Substances 0.000 claims description 111
- 238000007789 sealing Methods 0.000 claims description 80
- 239000000645 desinfectant Substances 0.000 claims description 32
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 238000007605 air drying Methods 0.000 claims description 3
- 230000000249 desinfective effect Effects 0.000 claims description 3
- 230000001502 supplementing effect Effects 0.000 claims description 2
- 238000007872 degassing Methods 0.000 claims 1
- 238000009434 installation Methods 0.000 claims 1
- 238000004113 cell culture Methods 0.000 abstract description 15
- 230000012010 growth Effects 0.000 abstract description 3
- 238000012258 culturing Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- 239000013589 supplement Substances 0.000 description 4
- 239000012535 impurity Substances 0.000 description 2
- 241000700605 Viruses Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 238000002309 gasification Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/22—Phase substances, e.g. smokes, aerosols or sprayed or atomised substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/20—Gaseous substances, e.g. vapours
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/26—Accessories or devices or components used for biocidal treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2101/00—Chemical composition of materials used in disinfecting, sterilising or deodorising
- A61L2101/32—Organic compounds
- A61L2101/34—Hydroxy compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
- A61L2202/10—Apparatus features
- A61L2202/15—Biocide distribution means, e.g. nozzles, pumps, manifolds, fans, baffles, sprayers
Abstract
The invention relates to the technical field of cell culture rack sterilization, in particular to a sterilization device and a sterilization method for cell collection and culture. The device comprises a culture table and a culture rack arranged inside the culture table, wherein an inflatable atomizer is arranged on one side of the culture table, and the inflatable atomizer is communicated with the culture table through an air duct. According to the invention, the disinfection gas is pumped into the cavity between the culture frame and the sliding plate through the inflatable atomizer, so that the disinfection gas disinfects the cavity, and the culture frame is pushed to move downwards along with the increase of the gas, so that the placement frame is immersed into the disinfection solution, the disinfection solution disinfects the placement frame in an omnibearing manner, the influence of disinfection dead angles on the cell culture is avoided, meanwhile, in the process of moving downwards the sliding plate, the sliding plate rubs with the side wall of the culture frame, the side wall of the culture frame rubs, and the disinfection gas is used for disinfection, so that the test cavity is disinfected, the cell culture in a sterile environment is ensured, and the normal growth of the cell is ensured.
Description
Technical Field
The invention relates to the technical field of cell culture rack sterilization, in particular to a sterilization device and a sterilization method for cell collection and culture.
Background
In the process of culturing cells, the cells need to grow in a sterile environment, so that normal culturing of the cells is ensured, when the cells are cultured, a cell culture dish is placed on a cell culture rack for culturing the cells, and after one-time culturing is completed, the cell culture rack needs to be sterilized, so that the cell culture rack can not generate infection among the cells when culturing new cells.
At present, when disinfecting the cell culture frame, most adopt the purple light lamp to carry out to advancing stoving, and adopt the purple light lamp to disinfect the cell culture frame when, the cell culture frame arc appears shining the dead angle, leads to the cell culture frame unable to be disinfected completely, and then appears the virus easily and remain, leads to when the cell culture frame is cultivateing the cell, appears the virus infection between the cell easily, influences the normal growth of cell.
Disclosure of Invention
The invention aims to provide a disinfection device and a disinfection method for cell collection and cultivation, which are used for solving the problems in the background technology.
In order to achieve the above object, one of the purposes of the present invention is to provide a disinfection device for cell collection and cultivation, which comprises a cultivation table and a cultivation frame arranged in the cultivation table, wherein one side of the cultivation table is provided with an inflatable atomizer, the inflatable atomizer is communicated with the cultivation table through an air duct, the cultivation table comprises a cultivation frame, a disinfection cavity and a test cavity are arranged in the cultivation frame, the disinfection cavity is arranged at the lower side of the test cavity, a disinfection frame is arranged in the disinfection cavity, a plurality of through holes are formed in the side wall of the disinfection frame, disinfection liquid is arranged in the disinfection cavity, the cultivation frame comprises a sliding plate, a placement frame is fixed at the lower side of the sliding plate, the sliding plate is arranged in the cultivation frame in a sliding manner, two sides of the cultivation frame are fixedly provided with inclined tubes, one ends of the inclined tubes are fixedly provided with liquid guide tubes, one ends of the two liquid guide tubes are connected with liquid collecting shells, one sides of the inflatable atomizer are provided with liquid collecting shells, the liquid collecting shells are communicated with the inflatable atomizer, a plurality of through holes are formed in the top of the cultivation frame, the sliding plate and the air guide plates are arranged at the top of the cultivation frame, one ends of the sliding plate are connected with the air guide tubes, one ends of the inclined tubes are connected with the hose and the hose through the control plate and the hose.
As a further improvement of the technical scheme, a drain pipe is fixed at the bottom of the disinfection frame, and one end of the drain pipe penetrates through the bottom of the cultivation frame and is provided with a sealing valve.
As a further improvement of the technical scheme, the inclined tube is communicated with the disinfection cavity, the inclined tube is obliquely upwards arranged, and one end of the liquid guide tube, which is far away from the inclined tube, is obliquely downwards arranged.
As a further improvement of the technical scheme, the upper side of the inclined tube is provided with an air outlet tube, the bottom of the culture frame and the bottom of the test cavity are communicated, one end of the air outlet tube, which is far away from the culture frame, is obliquely upwards arranged, one end of the air outlet tube is provided with a rubber sealing tube, and one end of the rubber sealing tube is flat.
As a further improvement of the technical scheme, pressing columns are fixed at four corners of the bottom of the sliding plate, upright posts are fixed at four corners of the bottom of the culture frame, and one end of each pressing column is inserted into each upright post.
As a further improvement of the technical scheme, the stand column is of a hollow structure, and a jacking spring is arranged in the stand column, and one end of the pressing column is fixedly connected with one end of the jacking spring.
As a further improvement of the technical scheme, limit strips are fixed on the left side wall and the right side wall of the test cavity, limit grooves are formed in the side walls of the sliding plates, and the limit strips are arranged in the limit grooves in a sliding mode.
As the further improvement of this technical scheme, be provided with sealing device on the culture rack, sealing device is including sealing the frame and fixing the return spring in sealing frame bottom four corners department, the inside wall of return spring and the lateral wall bottom side wall contact of rack, the one end of pressure post runs through sealing the frame and extends away, the tip at the stand is fixed to the one end of return spring.
As a further improvement of the technical scheme, a test port communicated with the test cavity is formed in the side wall of the culture frame, and a sealing plate is arranged in the test port.
The second object of the present invention is to provide a cell collection and culture disinfection method, comprising any one of the above disinfection device for cell collection and culture, comprising the following steps:
s1, sealing a test port by using a sealing plate, absorbing alcohol in a liquid collecting shell by using an inflatable atomizer, atomizing the alcohol, guiding atomized alcohol and gas into a cavity between a sliding plate and a culture frame by using an air duct, and enabling the gas to push the sliding plate to move downwards along with the continuous increase of the gas in the cavity, wherein the downwards moved sliding plate drives a placing frame and a pressing column to move downwards, so that the pressing column presses a pressing spring, and the pressing spring is contracted;
s2, in the process of downward movement of the sliding plate, air on the lower side of the sliding plate enters the rubber sealing tube through the air outlet tube, and the narrow opening of the rubber sealing tube is extruded and opened, so that gas leaves the device through the rubber sealing tube, when no gas flows out of the air outlet tube, the rubber sealing tube closes the narrow opening, the downward movement of the sliding plate drives the placing frame to enter the sterilizing frame, so that sterilizing liquid in the sterilizing frame is contacted with the placing frame, and the placing frame is sterilized;
s3, pressing the sealing frame by the sliding plate which moves downwards, enabling the sealing frame to move downwards along with the sliding plate, extruding and shrinking the return spring, and when the placing frame enters the disinfectant in the disinfection frame, raising the liquid level of the disinfectant, enabling part of disinfectant to flow into the liquid guide tube and the liquid collecting shell through the inclined tube, and supplementing the disinfectant in the liquid collecting shell;
s4, after the disinfection of the placing frame is completed, the inflatable atomizer stops inputting disinfection liquid and gas of atomized liquid into a cavity between the sliding plate and the culture frame, a control valve on a hose is opened, so that the disinfection gas between the sliding plate and the culture frame enters the test cavity, when the gas between the sliding plate and the culture frame is reduced, the top pressure spring begins to relax, the pressure column is pushed to move upwards, the moving-upwards pressure column drives the placing frame and the sliding plate to move upwards, the placing frame is enabled to leave disinfection liquid in the disinfection frame, in the process of moving upwards the culture frame, the sealing valve is opened, the disinfection liquid in the disinfection frame flows out of the placing frame through the drain pipe, the gas coming out of the hose blows the placing frame, air drying of the placing frame is quickened, and after the sliding plate leaves the sealing frame, the return spring pushes the sealing frame to move upwards, and after the sliding plate moves upwards to a designated position, the bottoms of the sealing frame and the placing frame are flush.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the disinfection device and the disinfection method for cell collection and cultivation, disinfection gas is pumped into the cavity between the cultivation frame and the sliding plate through the set inflatable atomizer, so that the disinfection gas disinfects the cavity, the cultivation frame is pushed to move downwards along with the increase of the gas, the placement frame is immersed into disinfection liquid, the disinfection liquid disinfects the placement frame in an omnibearing manner, the influence on the cultivation of cells due to the occurrence of disinfection dead angles is avoided, meanwhile, in the process of moving downwards the sliding plate, the sliding plate rubs with the side wall of the cultivation frame, the side wall of the cultivation frame rubs, disinfection gas is used for disinfection, the test cavity is disinfected, the cell cultivation is guaranteed in a sterile environment, and the normal growth of the cells is guaranteed.
2. According to the disinfection device and the disinfection method for cell collection and cultivation, the disinfection frame is arranged in the disinfection cavity, so that the placement frame enters the disinfection frame for disinfection, disinfection liquid for disinfection of the placement frame is led out of the disinfection frame by opening the sealing valve after disinfection of the placement frame is completed, the disinfection liquid leading-out device for disinfection of the placement frame after disinfection is completed is achieved, impurities left in the disinfection liquid are prevented from diffusing in the disinfection cavity, the effect of the disinfection liquid on the placement frame is reduced after repeated disinfection of the placement frame is avoided, and the disinfection effect of the disinfection liquid on the placement frame is ensured.
3. According to the disinfection device and the disinfection method for cell collection and cultivation, the placing frame is placed into the disinfection liquid, so that the liquid level of the disinfection liquid is raised, part of the disinfection liquid is placed into the liquid collecting shell, the inflatable atomizer is enabled to supplement the disinfection liquid, normal operation of the inflatable atomizer is guaranteed, meanwhile, after the placing frame leaves the disinfection liquid, part of the disinfection liquid in the liquid collecting shell flows back into the disinfection cavity, the disinfection liquid is supplemented to the disinfection cavity, and waste of the disinfection liquid is reduced.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is a schematic overall sectional view of the present invention;
FIG. 3 is a schematic cross-sectional combined structure of the culture table and the inflatable atomizer of the present invention;
FIG. 4 is a schematic view of a culture table according to the present invention;
FIG. 5 is a schematic cross-sectional view of a culture table according to the present invention;
FIG. 6 is a second schematic cross-sectional view of the culture table of the present invention;
FIG. 7 is a schematic view of the structure of the culture rack of the present invention;
fig. 8 is a schematic structural view of the sealing device of the present invention.
The meaning of each reference sign in the figure is:
1. a culture table; 11. a culture frame; 111. an air outlet pipe; 112. sealing the pipe with rubber; 12. a sterilization frame; 13. a catheter; 14. a chute; 15. a liquid collecting shell; 16. a drain pipe; 17. a column; 171. a pressing spring; 18. a limit bar; 19. a test port;
2. a culture rack; 21. a sliding plate; 22. a placing rack; 23. pressing a column; 24. a limit groove;
3. an inflatable atomizer;
4. a sealing device; 41. sealing a frame; 42. a return spring;
5. an air duct; 6. a sealing valve; 7. and (5) sealing the plate.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
Example 1
Referring to fig. 1-8, one of the purposes of this embodiment is to provide a disinfection device for cell collection and cultivation, which comprises a cultivation table 1 and a cultivation frame 2 arranged inside the cultivation table 1, wherein one side of the cultivation table 1 is provided with an inflatable atomizer 3, the inflatable atomizer 3 is communicated with the cultivation table 1 through an air duct 5, the cultivation table 1 comprises a cultivation frame 11, a test port 19 communicated with a test cavity is formed on the side wall of the cultivation frame 11, and a sealing plate 7 is arranged inside the test port 19;
the inside of the culture frame 11 is provided with a disinfection cavity and a test cavity, the disinfection cavity is arranged at the lower side of the test cavity, a disinfectant is arranged in the disinfection cavity, the inside of the disinfection cavity is provided with a disinfection frame 12, in order to make the disinfectant in the disinfection cavity enter the disinfection frame 12 to supplement the disinfectant in the disinfection frame 12, a plurality of through holes are formed in the side wall of the disinfection frame 12, the disinfectant in the disinfection cavity enters the disinfection frame 12 through the through holes, and the disinfectant in the disinfection frame 12 is supplemented, so that the disinfectant in the disinfection frame 12 sufficiently disinfects the placing frame 22 for the next time, and the normal disinfection of the placing frame 22 is ensured;
the culture rack 2 comprises a sliding plate 21, a placing rack 22 is fixed on the lower side of the sliding plate 21, the sliding plate 21 is arranged in the culture rack 11 in a sliding manner, inclined pipes 14 are fixed on two sides of the culture rack 11, the inclined pipes 14 are communicated with a disinfection cavity, liquid guide pipes 13 are fixed at one ends of the inclined pipes 14, one ends of the two liquid guide pipes 13 are connected with liquid collecting shells 15, the liquid collecting shells 15 are arranged on one side of the inflatable atomizer 3, the liquid collecting shells 15 are communicated with the inflatable atomizer 3, a cavity is arranged between the sliding plate 21 and the top of the culture rack 11, one end of an air guide pipe 5 is connected with the cavity between the sliding plate 21 and the culture rack 11, and in order to enable air between the sliding plate 21 and the culture rack 11 to be discharged, so that the culture rack 2 can return to the original position after disinfection is finished, an air outlet pipe 111 is arranged on the upper side of the inclined pipes 14, the bottom of the culture rack 11 is communicated with the test cavity, one end of the test cavity is arranged at the end of the test cavity, the air outlet pipe 111 is arranged upwards, one end of the air outlet pipe 111 is arranged at the end far from the culture rack 11, and a rubber sealing tube 112 is arranged at the end of the air outlet pipe 111;
considering that the sterilizing liquid in the sterilizing frame 12 can reduce the sterilizing capacity of the sterilizing liquid after sterilizing the placing frame, a drain pipe 16 for guiding the sterilizing liquid sterilized by the placing frame 22 in the sterilizing frame 12 is fixed at the bottom of the sterilizing frame 12, the sterilizing liquid in the sterilizing basket 12 is guided away from the sterilizing frame 12, meanwhile, one end of the drain pipe 16 penetrates through the bottom of the culturing frame 11 and is provided with a sealing valve 6, the flow of the sterilizing liquid in the drain pipe 16 is controlled, after the placing frame 22 is sterilized, the sealing valve 6 is opened, the sterilizing liquid in the sterilizing frame 12 flows out, so that the impurities remained in the sterilizing liquid are prevented from diffusing in a sterilizing cavity, the effect of the sterilizing liquid on the placing frame 22 is reduced after repeated sterilization of the placing frame 22 is avoided, and the sterilizing effect of the sterilizing liquid on the placing frame 22 is ensured;
the bottom four corners department of sliding plate 21 is fixed with press post 23, and the four corners department of cultivation frame 11 bottom is fixed with stand 17, and press post 23's one end is pegged graft in stand 17, and stand 17 is hollow structure, and the inside of stand 17 is provided with the roof pressure spring 171, and press post 23's one end and the one end fixed connection of roof pressure spring 171.
When the culture table 1 and the culture rack 2 are used, the sealing plate 7 is placed into the test port 19, the test port 19 is sealed, the culture frame 11 is isolated from the outside, the inflatable atomizer 3 absorbs alcohol in the liquid collecting shell 15, atomizes the alcohol, guides the atomized alcohol and gas into a cavity between the sliding plate 21 and the culture frame 11 through the gas guide pipe 5, and pushes the sliding plate 21 to move downwards along with the continuous increase of the gas in the cavity, the sliding plate 21 and the side wall of the culture frame 11 are rubbed to rub the side wall of the culture frame 11, and the sterilizing gas is used to sterilize the test cavity, so that the cells are cultured in a sterile environment, and the normal growth of the cells is ensured;
the sliding plate 21 which moves downwards drives the placing frame 22 and the pressing column 23 to move downwards, so that the pressing column 23 presses the pressing spring 171, the pressing spring 171 is contracted, in the process of moving downwards the sliding plate 21, air at the lower side of the sliding plate 21 enters the rubber sealing tube 112 through the air outlet tube 111, gas leaves the device through the rubber sealing tube 112, the sliding plate 21 which moves downwards drives the placing frame 22 to enter the sterilizing frame 12, sterilizing liquid in the sterilizing frame 12 contacts with the placing frame 22, sterilizing the placing frame 22, fully sterilizing the placing frame 22 by the sterilizing liquid, avoiding the influence on the culture of cells due to the occurrence of sterilizing dead angles, and when the placing frame 22 enters the sterilizing liquid in the sterilizing frame 12, the liquid level of the sterilizing liquid is raised, part of the sterilizing liquid flows into the liquid guide tube 13 and the liquid collecting shell 15 through the inclined tube 14, and the sterilizing liquid in the liquid collecting shell 15 is supplemented;
after the disinfection of the placing frame 22 is completed, the inflatable atomizer 3 stops inputting atomized disinfection liquid and gas into the cavity between the sliding plate 21 and the culture frame 11, and opens a control valve on a hose to enable the disinfection gas between the sliding plate 21 and the culture frame 11 to enter into a test cavity, so that the gas between the sliding plate 21 and the culture frame 11 is reduced, at the moment, the pressure of the extrusion top pressure spring 171 is reduced, the top pressure spring 171 begins to relax, the relaxed top pressure spring 171 pushes the pressure column 23 to move upwards, the upward moving pressure column 23 drives the placing frame 22 and the sliding plate 21 to move upwards, the placing frame 22 is separated from the disinfection liquid in the disinfection frame 12, and in the process of moving upwards the culture frame 2;
at this time, the disinfectant in the liquid collecting shell 15 flows back into the disinfection cavity, the disinfectant in the disinfection cavity is supplemented, the sealing valve 6 is opened, the disinfectant in the disinfection frame 12 flows out of the device through the drain pipe 16, the disinfectant in the disinfection cavity flows into the disinfection frame 12, the disinfection frame 12 is supplemented with the disinfectant, and the placing frame 22 is blown by the gas coming out of the hose, so that the placing frame 22 is air-dried, and after the disinfection is completed, the cell culture can be rapidly performed.
Considering that when the placing frame 22 enters the sterilizing frame 12, the sterilizing liquid entering the inclined tube 14 cannot flow into the liquid collecting shell 15 quickly, so that the sterilizing liquid is collected in the liquid guide tube 13, and the sterilizing liquid cannot supplement the liquid collecting cavity 15, the inclined tube 14 is obliquely upwards arranged, so that the inclined tube 14 guides the sterilizing liquid in the sterilizing cavity into the liquid guide tube 13, and meanwhile, one end of the liquid guide tube 13 away from the inclined tube 14 is obliquely downwards arranged, so that after the liquid in the sterilizing cavity enters the liquid guide tube 13, the sterilizing liquid can enter the liquid collecting shell 15 along the oblique direction of the liquid guide tube 13, and the sterilizing liquid in the liquid collecting shell 15 can be conveniently supplemented.
Example 2
Considering that after the air outlet pipe 111 is exhausted in the culture frame 11, the air outside the device flows back into the culture frame 11 through the rubber sealing pipe 112 to affect the air inside the culture frame 11, referring to fig. 5, one end of the rubber sealing pipe 112 is set to be flat, so that the inside of the rubber sealing pipe 112 is in a closed state when no air flows, the air inside and outside of the culture frame 11 are ensured to be isolated, and meanwhile, when the air outlet pipe 111 is exhausted, the air can normally come out, one end of the rubber sealing pipe 112 is provided with a narrow opening, when the air outlet pipe 111 is exhausted, the air is pushed out of the narrow opening, so that the air flows out, and when the air outlet pipe 111 is not exhausted, the narrow opening of the rubber sealing pipe 112 is closed, the air outside is blocked, the air outside is prevented from entering the culture frame 11, and the normal disinfection of the culture frame 11 is ensured.
Example 3
Considering that the sliding plate 21 is deviated when sliding in the culture frame 11, the sliding plate 21 cannot slide up and down in the culture frame 11 normally, and the placing frame 22 cannot enter into the sterilizing liquid for sterilization, referring to fig. 3-7, the limiting strips 18 are fixed on the left and right side walls of the test chamber, and the limiting grooves 24 are formed on the corresponding side walls of the sliding plate 21, so that the limiting strips 18 can slide in the limiting grooves 24, the limiting strips 18 limit the limiting grooves 24, the sliding position of the sliding plate 21 in the culture frame 11 is limited, the stable sliding of the sliding plate 21 is ensured, the inclination condition cannot occur when the sliding plate 21 slides up and down, and the normal sliding of the culture frame 2 in the culture frame 11 is ensured.
Example 4
Considering that in the process that the placing frame 22 normally cultures cells, the gasification of the disinfection liquid in the disinfection cavity is diffused into the test cavity through the gap between the placing frame 22 and the culture frame 11, the effect on the cells cultured on the placing frame 22 is achieved, referring to fig. 2 and 8, a sealing device 4 for sealing the gap between the culture frame 11 and the placing frame 22 is arranged on the culture frame 2, the sealing device 4 comprises a sealing frame 41 and return springs 42 fixed at four corners of the bottom of the sealing frame 41, the inner side walls of the return springs 42 are in contact with the bottom side walls of the placing frame 22, one ends of the pressing columns 23 penetrate through the sealing frame 41 and extend out, one ends of the return springs 42 are fixed at the end parts of the upright posts 17, after the placing frame 22 is disinfected, the sealing frame 41 moves upwards along with the sliding plate 21, and after the placing frame 22 moves upwards to a designated position, the bottoms of the sealing frame 41 and the placing frame 22 are flush, the placing frame 22 and the sealing frame 41 cooperate to isolate the liquid in the disinfection cavity, the diffusion of the disinfection liquid is reduced, and the normal operation of the test is ensured.
Meanwhile, in order to supplement the disinfectant in the disinfection cavity, an adding pipe is arranged on the liquid collecting shell 15, and the disinfectant flows into the disinfection cavity through the liquid guide pipe 13 by adding the disinfectant into the adding pipe, so that the disinfectant in the disinfection cavity is conveniently supplemented.
The second object of the present invention is to provide a cell collection and culture disinfection method, comprising any one of the above disinfection device for cell collection and culture, comprising the following steps:
s1, sealing a test port 19 by using a sealing plate 7, absorbing alcohol in a liquid collecting shell 15 by an inflatable atomizer 3, atomizing the alcohol, introducing atomized alcohol and gas into a cavity between a sliding plate 21 and a culture frame 11 through a gas guide pipe 5, and enabling the gas to push the sliding plate 21 to move downwards along with the continuous increase of the gas in the cavity, wherein the sliding plate 21 which moves downwards drives a placing frame 22 and a pressing column 23 to move downwards, so that the pressing column 23 presses a pressing spring 171, and the pressing spring 171 contracts;
s2, in the process of downward movement of the sliding plate 21, air on the lower side of the sliding plate 21 enters the rubber sealing tube 112 through the air outlet tube 111, and the narrow opening of the rubber sealing tube 112 is extruded and opened, so that gas leaves the device through the rubber sealing tube 112, when no gas flows out of the air outlet tube 111, the narrow opening is closed by the rubber sealing tube 112, the downward movement of the sliding plate 21 drives the placing frame 22 to enter the sterilizing frame 12, so that sterilizing liquid in the sterilizing frame 12 contacts with the placing frame 22, and sterilization is carried out on the placing frame 22;
s3, the sliding plate 21 which moves downwards presses the sealing frame 41, so that the sealing frame 41 moves downwards along with the sliding plate 21, the return spring 42 is extruded and contracted, when the placing frame 22 enters the disinfectant in the disinfection frame 12, the liquid level of the disinfectant is increased, part of disinfectant flows into the liquid guide tube 13 and the liquid collecting shell 15 through the inclined tube 14, and the disinfectant in the liquid collecting shell 15 is replenished;
s4, after the disinfection of the placing frame 22 is completed, the inflatable atomizer 3 stops inputting disinfection liquid and gas of atomized liquid into the cavity between the sliding plate 21 and the cultivating frame 11, a control valve on a hose is opened, the disinfection gas between the sliding plate 21 and the cultivating frame 11 enters into the test cavity, when the gas between the sliding plate 21 and the cultivating frame 11 is reduced, the top pressure spring 171 begins to relax, the pressure column 23 is pushed to move upwards, the upward moving pressure column 23 drives the placing frame 22 and the sliding plate 21 to move upwards, the placing frame 22 is separated from the disinfection liquid in the disinfection frame 12, in the process of moving upwards the cultivating frame 2, the sealing valve 6 is opened, the disinfection liquid in the disinfection frame 12 flows out of the device through the drain pipe 16, the air from the hose blows the placing frame 22, the air drying of the placing frame 22 is quickened, after the sliding plate 21 is separated from the unsealing frame 41, the sealing frame 41 is pushed to move upwards by the return spring 42, and when the sliding plate 21 moves upwards to a designated position, the sealing frame 41 and the bottom of the placing frame 22 are flush.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the above-described embodiments, and that the above-described embodiments and descriptions are only preferred embodiments of the present invention, and are not intended to limit the invention, and that various changes and modifications may be made therein without departing from the spirit and scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (7)
1. Cell collection is cultivated and is used degassing unit, including cultivateing platform (1) and setting up at cultivateing inside cultivateing frame (2) of platform (1), one side of cultivateing platform (1) is provided with inflatable atomizer (3), be linked together through air duct (5) between inflatable atomizer (3) and the cultivateing platform (1), its characterized in that: the culture table (1) comprises a culture frame (11), a disinfection cavity and a test cavity are arranged in the culture frame (11), the disinfection cavity is arranged on the lower side of the test cavity, the disinfection cavity is internally provided with a disinfection frame (12), a plurality of through holes are formed in the side wall of the disinfection frame (12), disinfectant is arranged in the disinfection cavity, the culture frame (2) comprises a sliding plate (21), a placing frame (22) is fixed on the lower side of the sliding plate (21), the sliding plate (21) is arranged in the culture frame (11) in a sliding mode, inclined tubes (14) are fixed on two sides of the culture frame (11), a liquid collecting shell (15) is fixed at one end of each inclined tube (14), a liquid collecting shell (15) is arranged on one side of each gas-filled atomizer (3), the liquid collecting shell (15) is communicated with the gas-filled atomizer (3), a cavity (21) is arranged between the sliding plate (21) and the top of the culture frame (11), an inclined tube (14) is fixed on two sides of the inclined tubes (14), one end of each inclined tube (13) is connected with a liquid collecting shell (15), and the other is connected with the corresponding hose (11) through the corresponding to the corresponding control valve, and the cavity (11) through the control valve (11);
a drain pipe (16) is fixed at the bottom of the disinfection frame (12), and one end of the drain pipe (16) penetrates through the bottom of the cultivation frame (11) and is provided with a sealing valve (6);
the four corners of the bottom of the sliding plate (21) are fixedly provided with pressing columns (23), the four corners of the bottom of the culture frame (11) are fixedly provided with upright posts (17), and one end of each pressing column (23) is inserted into each upright post (17);
the stand (17) is of a hollow structure, a jacking spring (171) is arranged in the stand (17), and one end of the pressing column (23) is fixedly connected with one end of the jacking spring (171).
2. The cell collection and culture sterilizer according to claim 1, wherein: the inclined tube (14) is communicated with the disinfection cavity, the inclined tube (14) is obliquely upwards arranged, and one end, far away from the inclined tube (14), of the liquid guide tube (13) is obliquely downwards arranged.
3. The cell collection and culture sterilizer according to claim 1, wherein: the upper side of inclined tube (14) is provided with outlet duct (111), the bottom of cultivation frame (11) and test chamber is linked together, the one end slope that cultivation frame (11) was kept away from to outlet duct (111) upwards sets up, one end installation of outlet duct (111) is connected with rubber tube seal (112), the one end of rubber tube seal (112) is the platykurtic.
4. The cell collection and culture sterilizer according to claim 1, wherein: limiting strips (18) are fixed on the left side wall and the right side wall of the test cavity, limiting grooves (24) are formed in the side walls of the sliding plates (21), and the limiting strips (18) are arranged in the limiting grooves (24) in a sliding mode.
5. The cell collection and culture sterilizer according to claim 1, wherein: the culture rack (2) is provided with a sealing device (4), the sealing device (4) comprises a sealing frame (41) and return springs (42) fixed at four corners of the bottom of the sealing frame (41), the inner side walls of the return springs (42) are contacted with the bottom side walls of the placing rack (22), one end of the pressing column (23) penetrates through the sealing frame (41) and extends out, and one end of the return springs (42) is fixed at the end part of the upright column (17).
6. The cell collection and culture sterilizer according to claim 1, wherein: a test port (19) communicated with the test cavity is formed in the side wall of the culture frame (11), and a sealing plate (7) is arranged in the test port (19).
7. A method for sterilizing a cell collection/culture system, comprising the cell collection/culture sterilizing apparatus according to any one of claims 1 to 6, characterized in that: the method comprises the following steps:
s1, sealing a test port (19) by using a sealing plate (7), absorbing alcohol in a liquid collecting shell (15) by an inflatable atomizer (3), atomizing the alcohol, guiding atomized alcohol and gas into a cavity between a sliding plate (21) and a culture frame (11) by using an air duct (5), and pushing the sliding plate (21) to move downwards along with the continuous increase of the gas in the cavity, wherein the sliding plate (21) which moves downwards drives a placing frame (22) and a pressing column (23) to move downwards, so that the pressing column (23) presses a pressing spring (171), and the pressing spring (171) is contracted;
s2, in the process of downward movement of the sliding plate (21), air at the lower side of the sliding plate (21) enters the rubber sealing tube (112) through the air outlet tube (111), and the narrow opening of the rubber sealing tube (112) is extruded and opened, so that gas leaves the device through the rubber sealing tube (112), when no gas flows out of the air outlet tube (111), the narrow opening is closed by the rubber sealing tube (112), the downward movement of the sliding plate (21) drives the placing frame (22) to enter the sterilizing frame (12), so that sterilizing liquid in the sterilizing frame (12) is contacted with the placing frame (22), and the placing frame (22) is sterilized;
s3, pressing the sealing frame (41) by the sliding plate (21) which moves downwards, enabling the sealing frame (41) to move downwards along with the sliding plate (21), extruding and contracting the return spring (42), and when the placing frame (22) enters the disinfectant in the disinfection frame (12), increasing the liquid level of the disinfectant, enabling part of the disinfectant to flow into the liquid guide tube (13) and the liquid collecting shell (15) through the inclined tube (14), and supplementing the disinfectant in the liquid collecting shell (15);
s4, after disinfection of the placing frame (22) is completed, the inflatable atomizer (3) stops inputting disinfectant liquid and gas of atomized liquid into the cavity between the sliding plate (21) and the cultivating frame (11), a control valve on a hose is opened, the disinfectant gas between the sliding plate (21) and the cultivating frame (11) enters into a test cavity, when the gas between the sliding plate (21) and the cultivating frame (11) is reduced, the top pressure spring (171) begins to relax, the pressure column (23) is pushed to move upwards, the upward moving pressure column (23) drives the placing frame (22) and the sliding plate (21) to move upwards, the placing frame (22) is separated from disinfectant liquid in the disinfecting frame (12), in the process of moving upwards of the cultivating frame (2), the sealing valve (6) is opened, the disinfectant liquid in the disinfecting frame (12) flows out of the device through the drain pipe (16), the air drying of the placing frame (22) is accelerated, after the sliding plate (21) leaves the sealing frame (41), the return spring (42) pushes the placing frame (41) to move upwards, and the sealing frame (41) is moved to the appointed position after the sealing frame (41) is flushed.
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