CN112517102A

CN112517102A - Many specifications are heat preservation centrifuging tube support for cell culture

Info

Publication number: CN112517102A
Application number: CN202010552391.3A
Authority: CN
Inventors: 徐苏芮; 李翔; 毕国强; 刘北明
Original assignee: University of Science and Technology of China USTC
Current assignee: University of Science and Technology of China USTC
Priority date: 2020-06-17
Filing date: 2020-06-17
Publication date: 2021-03-19

Abstract

The invention discloses a heat-preservation centrifuge tube bracket for multi-specification cell culture, which comprises a centrifuge tube cavity, a reagent bottle cavity and a supporting heat-preservation part, wherein the tops of the centrifuge tube cavity and the reagent bottle cavity are open, solid side wall structures are arranged below the open, the centrifuge tube cavity has three specifications, commonly used centrifuge tubes of 1.5ml,15ml and 50ml can be placed in the centrifuge tube cavity respectively, a reagent bottle with the bottom of 500ml and a cuboid can be placed in the reagent bottle cavity, the supporting heat-preservation part is a cuboid in the shape of the outer wall, the side walls combining the centrifuge tube cavity and the reagent bottle cavity are of an integrated structure, the middle part of the supporting heat-preservation part is in a vacuum state, and the bottom. The centrifugal tube rack can be used for placing centrifugal tubes with various specifications and reagent bottles, so that the experimental space is saved, and experimenters can complete experimental operation orderly. In addition, the placement of the bracket is reduced, so that the sterilization is more sufficient, and the possibility of pollution is reduced. Secondly, the transparent material makes the laboratory technician can be fine control reagent liquid level's height in the operation process.

Description

Many specifications are heat preservation centrifuging tube support for cell culture

Technical Field

The invention relates to the technical field of cell culture, in particular to a heat-preservation centrifuge tube bracket for multi-specification cell culture.

Background

Cell culture techniques are widely used in scientific research, and the suitable temperature for in vitro culture of mammalian and avian cells is about 37 ℃. In preparation for cell experiments, a series of reagents, such as culture medium, pancreatin, PBS, etc., are preheated and then sterilized on a test tube holder in a clean room. However, cell experiments such as passage, liquid change, etc. generally last for ten to tens of minutes depending on the sample amount and the experiment contents. The medium or other reagents heated in the water bath are cooled to room temperature, and a temperature below 37 ℃ is a stress to the cells.

The existing bracket only plays a role of supporting and supporting the centrifuge tube and cannot help to maintain the temperature of the reagent. And a plurality of supports are usually used in a cell culture super-clean bench, the common specifications are 1.5ml,15ml and 50ml, and too many supports are placed in the super-clean bench, so that the space is occupied, dead angles exist in the super-clean bench, ultraviolet rays and alcohol are difficult to sterilize, and bacterial pollution is easily caused. In addition, some holders are high and are not convenient for observing the liquid level.

Disclosure of Invention

The invention aims to maintain the temperature of a cell culture reagent in the experimental time, save space and reduce the possibility of pollution, and provides a multi-specification heat-preservation centrifugal tube support for cell culture. The temperature maintaining function of the support is added on the basis of a common support, so that the stress response of the temperature reduction of the reagent to cells is reduced in the cell culture process, and the cell culture is better. And the three supports are integrated, so that the space is saved, and the possibility of cell contamination is reduced. Although the support is high, the transparent material does not influence the height of the observed liquid level, and the support is convenient for a laboratory technician to operate.

The technical scheme adopted by the invention is as follows: a heat-preservation centrifuge tube bracket for multi-specification cell culture comprises a centrifuge tube cavity, a reagent bottle cavity and a supporting heat-preservation part, wherein the tops of the centrifuge tube cavity and the reagent bottle cavity are open, and an entity side wall structure is arranged below the open; the centrifugal tube cavity has three specifications, and common centrifugal tubes of 1.5ml,15ml and 50ml can be placed in the centrifugal tube cavity respectively. The reagent bottle cavity can be used for placing 500ml of reagent bottles with cuboid bottoms; the supporting heat-insulating part is in a cuboid shape on the outer wall, the side walls of the combined centrifugal tube cavity and the reagent bottle cavity are of an integrated structure, and the middle part of the supporting heat-insulating part is in a vacuum state; the bottom of the supporting and heat-insulating part is provided with four support legs.

Further, the centrifuging tube support is transparent PP material.

Further, the bottom of centrifugation lumen is the toper, and reagent bottle chamber is the cuboid, and the shape of whole centrifuging tube support is the cuboid.

Furthermore, the inner parts of the tube walls are combined into a whole, the middle part is in a vacuum state, and the temperature of the culture medium can be kept.

Compared with the prior art, the invention has the advantages that:

the heat-insulating centrifuge tube bracket for multi-specification cell culture provided by the invention can be used for placing centrifuge tubes of various specifications and reagent bottles, so that the experiment space is saved, and experimenters can complete experiment operation orderly. In addition, the placement of the bracket is reduced, so that the sterilization is more sufficient, and the possibility of pollution is reduced. Secondly, the transparent material makes the laboratory technician can be fine control reagent liquid level's volume in the operation process. Finally, the heat preservation function maintains the temperature of the reagent heated by the water bath. In the aspect of washing, the multilayer framework of general centrifuge tube rack makes the clearance dead angle many, and this support dead angle is few, facilitates the use test tube brush clearance. In addition, the integrated structure and the economical material make the manufacture simple.

Drawings

FIG. 1 is a schematic perspective view of a multi-specification rack for an incubation centrifugal tube for cell culture according to the present invention;

FIG. 2 is a top view of the multi-standard bracket for the thermal-insulation centrifuge tube for cell culture according to the present invention;

FIG. 3 is a schematic bottom view of the multi-standard rack for holding centrifuge tubes for cell culture according to the present invention;

FIG. 4 is a side view of the bottom of the multi-standard rack for holding centrifuge tubes for cell culture according to the present invention;

description of reference numerals:

1. a reagent bottle chamber; 2. 1.5ml conical centrifuge tube; 3. 15ml conical centrifuge tube holes; 4. 50ml conical centrifuge tube holes; 5. a supporting heat-insulating part; 6. a bottom leg.

Detailed Description

The present invention will be described in detail below with reference to specific embodiments shown in the drawings.

As shown in FIGS. 1 and 3, the invention relates to a multi-specification heat-preservation centrifuge tube bracket for cell culture, which comprises a centrifuge tube cavity, a reagent bottle cavity 1, a supporting heat-preservation part 5 and a bottom support leg 6. The centrifuge lumen comprises: 1.5ml conical centrifugation tube cavity 2, 15ml conical centrifugation tube cavity 3, 50ml conical centrifugation tube cavity 4.

The 1.5ml conical centrifugal tube cavity 2 is provided with a row of 16 holes; the 15ml conical centrifugal tube cavity 3 has two rows, each row has 4 holes, and the total number of the holes is 8; the 50ml conical centrifuge tube chamber 4 has two rows of 4 wells each, for a total of 8 wells. Wherein two rows of 50ml conical centrifuge chambers 4 are vertically arranged and two rows of 15ml conical centrifuge chambers 3 are arranged at an angle of 45 degrees.

The diameter of the 1.5ml conical centrifugal tube cavity 2 is 1.1cm, and the depth is 3 cm; the diameter of the 15ml conical centrifugal tube cavity 3 is 1.65cm, and the depth is 8.5 cm; the 50ml conical centrifuge tube lumen 4 has a diameter of 2.8cm and a depth of 8.5 cm. The reagent bottle cavity 1 is in a cuboid shape, the top end of the reagent bottle cavity is open, and the length, the width and the height of the reagent bottle cavity are respectively 7.8cm,7.8cm and 8.5 cm. The centrifugal tube cavity and the reagent bottle cavity 1 can be used for well placing corresponding reagent bottles.

The length of the outer wall of the supporting and heat-insulating part 5 is 25 cm, the width is 12cm, and the height is 10 cm. The bottom support leg 6 is made of silica gel and can prevent slipping. In addition, the height of the bracket is raised, and the contact with the bottom is reduced to reduce heat dissipation.

The centrifugal tube cavity, the reagent bottle cavity 1 and the supporting heat preservation part 5 are of an integrated structure and made of PP. The middle parts of the centrifugal tube cavity wall, the reagent bottle cavity 1 wall and the support heat preservation part 5 wall are in a vacuum state. The PP material is a poor heat conductor, and the vacuum does not conduct heat, so that the reagent heated in the water bath can be well insulated when placed in the bracket.

This support uses transparent PP material, the height of the actual liquid level of observation that can be better makes things convenient for the laboratory technician to absorb and add liquid.

These embodiments do not limit the present invention, and structural, methodological, or functional changes made according to these embodiments are included in the scope of the present invention.

Claims

1. The utility model provides a many specifications are heat preservation centrifuging tube support for cell culture which characterized in that: centrifuging tube support includes centrifugation lumen, reagent bottle chamber and supports heat preservation, wherein centrifugation lumen and reagent bottle chamber top are uncovered, be entity lateral wall structure below uncovered, the total three kinds of specifications of centrifugation lumen, can place commonly used 1.5ml respectively, 15ml and 50 ml's centrifuging tube, the reagent bottle that 500ml bottom is the cuboid can be placed to the reagent bottle chamber, it is the cuboid to support the heat preservation for the outer wall shape, the lateral wall in combination centrifugation lumen and reagent bottle chamber is structure as an organic whole, the centre is vacuum state, it is four stabilizer blades to support the heat preservation bottom.

2. The heat-preserving centrifuge tube bracket for multi-specification cell culture according to claim 1, which is characterized in that: the centrifuging tube support is transparent PP material.

3. The heat-preserving centrifuge tube bracket for multi-specification cell culture according to claim 1, which is characterized in that: the bottom of centrifugation tube chamber is the toper, and reagent bottle chamber is the cuboid, and the shape of whole centrifuging tube support is the cuboid.

4. The heat-preserving centrifuge tube bracket for multi-specification cell culture according to claim 1, which is characterized in that: the inner parts of the tube walls are combined into a whole, the middle part is in a vacuum state, and the temperature of the culture medium can be kept.