CN114766305A - Method for adjusting composition structure of rhizosphere microbial community for panax notoginseng seedling culture - Google Patents
Method for adjusting composition structure of rhizosphere microbial community for panax notoginseng seedling culture Download PDFInfo
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- CN114766305A CN114766305A CN202210595699.5A CN202210595699A CN114766305A CN 114766305 A CN114766305 A CN 114766305A CN 202210595699 A CN202210595699 A CN 202210595699A CN 114766305 A CN114766305 A CN 114766305A
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- composition
- adjusting
- rhizosphere
- panax notoginseng
- microbial community
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- 241000180649 Panax notoginseng Species 0.000 title claims abstract description 38
- 235000003143 Panax notoginseng Nutrition 0.000 title claims abstract description 38
- 230000000813 microbial effect Effects 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims abstract description 20
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 19
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 19
- 239000000126 substance Substances 0.000 claims abstract description 14
- 238000005507 spraying Methods 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000002689 soil Substances 0.000 claims abstract description 12
- 230000002378 acidificating effect Effects 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 10
- FJKROLUGYXJWQN-UHFFFAOYSA-N 4-hydroxybenzoic acid Chemical compound OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 claims abstract description 8
- KSEBMYQBYZTDHS-HWKANZROSA-M (E)-Ferulic acid Natural products COC1=CC(\C=C\C([O-])=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-M 0.000 claims abstract description 4
- 229940090248 4-hydroxybenzoic acid Drugs 0.000 claims abstract description 4
- KSEBMYQBYZTDHS-HWKANZROSA-N ferulic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-N 0.000 claims abstract description 4
- 229940114124 ferulic acid Drugs 0.000 claims abstract description 4
- KSEBMYQBYZTDHS-UHFFFAOYSA-N ferulic acid Natural products COC1=CC(C=CC(O)=O)=CC=C1O KSEBMYQBYZTDHS-UHFFFAOYSA-N 0.000 claims abstract description 4
- 235000001785 ferulic acid Nutrition 0.000 claims abstract description 4
- YQUVCSBJEUQKSH-UHFFFAOYSA-N protochatechuic acid Natural products OC(=O)C1=CC=C(O)C(O)=C1 YQUVCSBJEUQKSH-UHFFFAOYSA-N 0.000 claims abstract description 4
- QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 claims abstract description 4
- WKOLLVMJNQIZCI-UHFFFAOYSA-N vanillic acid Chemical compound COC1=CC(C(O)=O)=CC=C1O WKOLLVMJNQIZCI-UHFFFAOYSA-N 0.000 claims abstract description 4
- TUUBOHWZSQXCSW-UHFFFAOYSA-N vanillic acid Natural products COC1=CC(O)=CC(C(O)=O)=C1 TUUBOHWZSQXCSW-UHFFFAOYSA-N 0.000 claims abstract description 4
- 244000005700 microbiome Species 0.000 claims description 6
- 230000001105 regulatory effect Effects 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 5
- 239000013043 chemical agent Substances 0.000 abstract description 3
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 abstract description 3
- 230000001954 sterilising effect Effects 0.000 abstract description 3
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 3
- 244000131316 Panax pseudoginseng Species 0.000 description 7
- 235000003181 Panax pseudoginseng Nutrition 0.000 description 7
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 241000208173 Apiaceae Species 0.000 description 1
- 241000208340 Araliaceae Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a method for adjusting the composition structure of a rhizosphere microbial community of panax notoginseng seedling, which comprises the steps of preparing raw materials according to the seedling number, preparing an adjusting composition, adding bacillus subtilis, spraying a water source, adding the adjusting composition and finishing the composition of the rhizosphere microbial community of panax notoginseng seedling, wherein an acidic substance can be one of p-hydroxybenzoic acid, ferulic acid or vanillic acid. The method comprises the steps of preparing raw materials according to the number of seedlings, preparing an adjusting composition, adding bacillus subtilis, spraying a water source and adding the adjusting composition to adjust the composition structure of the rhizosphere microbial community of the panax notoginseng seedlings, adopting a chemical agent for sterilization, covering the rhizosphere of the panax notoginseng seedlings by the prepared adjusting composition, maintaining the rhizosphere microbes of the panax notoginseng seedlings, increasing the beneficial microbial community, and reducing phenolic substances in soil, so that the yield and the quality of the panax notoginseng are improved, and the method has a certain application prospect.
Description
Technical Field
The invention relates to the technical field of pseudo-ginseng seedling culture, in particular to a method for adjusting the rhizosphere microbial community composition structure of pseudo-ginseng seedling culture.
Background
Pseudo-ginseng, also known as pseudo-ginseng, is a perennial herb of panax notoginseng belonging to the family of araliaceae, order Umbelliferae, with a plant height of 30-60 cm, and its stem, leaf and flower can be used as medicine.
However, the growth cycle of pseudo-ginseng is long, various diseases are serious, a single chemical control technical measure is not only not beneficial to solving the problem of field diseases but also causes the increase of the drug resistance of the diseases, which seriously reduces the yield of pseudo-ginseng, thereby restricting the development of the pseudo-ginseng industry.
Disclosure of Invention
The technical problem to be solved by the invention is to overcome the existing defects and provide a method for adjusting the composition structure of the rhizosphere microbial community of panax notoginseng seedling culture, which can effectively solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a method for adjusting composition structure of microorganism community at rhizosphere of radix Notoginseng comprises preparing raw materials according to seedling number, preparing adjusting composition, adding Bacillus subtilis, spraying water source, adding adjusting composition, and completing composition of microorganism community at rhizosphere of radix Notoginseng;
the method for adjusting the composition structure of the rhizosphere microbial community for panax notoginseng seedling culture comprises the following steps:
step one, preparing raw materials according to the seedling number: preparing a certain amount of bacillus subtilis, alkaline soil and acidic substances in advance according to the seedling number;
step two, preparing a regulating composition: according to the following steps: 10, mixing the acidic substance into the alkaline soil, and uniformly stirring to prepare an adjusting composition;
step three, throwing bacillus subtilis and spraying a water source: adding bacillus subtilis into the soil according to the seedling number, and spraying a water source;
step four, adding a regulating composition: covering the root of the panax notoginseng seedlings with the adjusting composition;
and step five, finishing the composition of the rhizosphere microbial community of the panax notoginseng seedling culture: realizes the adjustment of the composition structure of the microbial community of the panax notoginseng seedling rhizosphere.
Preferably, the acidic substance may be one of p-hydroxybenzoic acid, ferulic acid or vanillic acid.
Preferably, 100 g of bacillus subtilis and 200 g of the adjusting composition are adopted for each seedling.
Compared with the prior art, the invention has the beneficial effects that:
the method comprises the steps of preparing raw materials according to the number of seedlings, preparing an adjusting composition, adding bacillus subtilis, spraying a water source and adding the adjusting composition to adjust the composition structure of the rhizosphere microbial community of the panax notoginseng seedlings, adopting a chemical agent for sterilization, covering the rhizosphere of the panax notoginseng seedlings by the prepared adjusting composition, maintaining the rhizosphere microbes of the panax notoginseng seedlings, increasing the beneficial microbial community, and reducing phenolic substances in soil, so that the yield and the quality of the panax notoginseng are improved, and the method has a certain application prospect.
Drawings
FIG. 1 is a flow chart of the method for adjusting the composition structure of microbial communities in the rhizosphere of panax notoginseng seedlings.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further explained by combining the specific embodiments.
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A method for adjusting composition structure of microorganism community at rhizosphere of radix Notoginseng comprises preparing raw materials according to seedling number, preparing adjusting composition, adding Bacillus subtilis, spraying water source, adding adjusting composition, and completing composition of microorganism community at rhizosphere of radix Notoginseng;
the method for adjusting the composition structure of the rhizosphere microbial community for panax notoginseng seedling culture comprises the following steps:
step one, preparing raw materials according to the seedling number: preparing a certain amount of bacillus subtilis, alkaline soil and acidic substances in advance according to the seedling number;
step two, preparing a regulating composition: according to the following steps: 10, mixing the acidic substance into the alkaline soil, and uniformly stirring to prepare an adjusting composition;
step three, throwing bacillus subtilis and spraying a water source: adding bacillus subtilis into the soil according to the seedling number, and spraying a water source;
step four, adding a regulating composition: covering the root of the panax notoginseng seedlings with the adjusting composition;
and step five, finishing the composition of the rhizosphere microbial community of the panax notoginseng seedling culture: realizes the adjustment of the composition structure of the microbial community of the panax notoginseng seedling rhizosphere.
Further, the acidic substance may be one of p-hydroxybenzoic acid, ferulic acid or vanillic acid.
Furthermore, 100 g of bacillus subtilis and 200 g of the adjusting composition are adopted for each seedling.
According to the method, the composition structure of the rhizosphere microbial community of the panax notoginseng seedlings is adjusted through the steps of preparing raw materials according to the seedling number, preparing an adjusting composition, adding bacillus subtilis, spraying a water source and adding the adjusting composition, the seedling rhizosphere is covered by the prepared adjusting composition after the sterilization of a chemical agent, the rhizosphere microbes of the panax notoginseng seedlings are maintained, the beneficial microbial community is increased, phenolic substances in soil are reduced, the yield and the quality of panax notoginseng are improved, and the method has a certain application prospect.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (3)
1. A method for adjusting composition structure of microorganism community at rhizosphere of radix Notoginseng comprises preparing raw materials according to seedling number, preparing adjusting composition, adding Bacillus subtilis, spraying water source, adding adjusting composition, and completing composition of microorganism community at rhizosphere of radix Notoginseng;
the method for adjusting the composition structure of the rhizosphere microbial community for panax notoginseng seedling culture comprises the following steps:
step one, preparing raw materials according to the seedling number: preparing a certain amount of bacillus subtilis, alkaline soil and acidic substances in advance according to the seedling number;
step two, preparing a regulating composition: according to the proportion of 1: 10, mixing the acidic substance into the alkaline soil, and uniformly stirring to prepare an adjusting composition;
step three, throwing bacillus subtilis and spraying a water source: adding bacillus subtilis into soil according to the seedling number, and spraying a water source;
step four, adding a regulating composition: covering the root of the panax notoginseng seedlings with the adjusting composition;
step five, finishing the rhizosphere microbial community composition of the panax notoginseng seedling culture: the composition structure of the microbial community of the rhizosphere of the panax notoginseng seedling is adjusted.
2. The method for adjusting the composition structure of the rhizosphere microbial community of panax notoginseng seedlings according to claim 1, wherein the acidic substance can be one of p-hydroxybenzoic acid, ferulic acid or vanillic acid.
3. The method for adjusting the rhizosphere microbial community composition structure of panax notoginseng seedlings according to claim 1, wherein 100 g of bacillus subtilis and 200 g of the adjusting composition are adopted for each seedling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210595699.5A CN114766305A (en) | 2022-05-27 | 2022-05-27 | Method for adjusting composition structure of rhizosphere microbial community for panax notoginseng seedling culture |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210595699.5A CN114766305A (en) | 2022-05-27 | 2022-05-27 | Method for adjusting composition structure of rhizosphere microbial community for panax notoginseng seedling culture |
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| CN114766305A true CN114766305A (en) | 2022-07-22 |
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|---|---|---|---|
| CN202210595699.5A Pending CN114766305A (en) | 2022-05-27 | 2022-05-27 | Method for adjusting composition structure of rhizosphere microbial community for panax notoginseng seedling culture |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105557252A (en) * | 2015-12-14 | 2016-05-11 | 云南农业大学 | Pesticide-fertilizer integrated pseudo-ginseng cultivation method |
| CN106332637A (en) * | 2016-08-25 | 2017-01-18 | 文山苗乡三七科技有限公司 | Method for cultivating radix notoginseng without pesticide residues |
| CN109169092A (en) * | 2018-07-27 | 2019-01-11 | 吉林冠州生物科技有限公司 | A method of reducing crop field ginseng continuous cropping obstacle |
| CN110423696A (en) * | 2019-08-22 | 2019-11-08 | 云南农业大学 | The trichoderma strain screening technique for preventing and treating notoginseng root rot |
| CN111732479A (en) * | 2020-07-06 | 2020-10-02 | 云南顺丰洱海环保科技股份有限公司 | Composite microbial organic fertilizer and preparation method thereof |
| CN111892449A (en) * | 2019-05-13 | 2020-11-06 | 昆明学院 | Preparation and application methods of pseudo-ginseng carbon-based biological enzyme fertilizer |
| CN112970780A (en) * | 2021-02-23 | 2021-06-18 | 中国医学科学院药用植物研究所 | Special microbial agent for ginseng and method for reducing incidence rate of ginseng rust rot |
-
2022
- 2022-05-27 CN CN202210595699.5A patent/CN114766305A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105557252A (en) * | 2015-12-14 | 2016-05-11 | 云南农业大学 | Pesticide-fertilizer integrated pseudo-ginseng cultivation method |
| CN106332637A (en) * | 2016-08-25 | 2017-01-18 | 文山苗乡三七科技有限公司 | Method for cultivating radix notoginseng without pesticide residues |
| CN109169092A (en) * | 2018-07-27 | 2019-01-11 | 吉林冠州生物科技有限公司 | A method of reducing crop field ginseng continuous cropping obstacle |
| CN111892449A (en) * | 2019-05-13 | 2020-11-06 | 昆明学院 | Preparation and application methods of pseudo-ginseng carbon-based biological enzyme fertilizer |
| CN110423696A (en) * | 2019-08-22 | 2019-11-08 | 云南农业大学 | The trichoderma strain screening technique for preventing and treating notoginseng root rot |
| CN111732479A (en) * | 2020-07-06 | 2020-10-02 | 云南顺丰洱海环保科技股份有限公司 | Composite microbial organic fertilizer and preparation method thereof |
| CN112970780A (en) * | 2021-02-23 | 2021-06-18 | 中国医学科学院药用植物研究所 | Special microbial agent for ginseng and method for reducing incidence rate of ginseng rust rot |
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Application publication date: 20220722 |
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