CN114762503A - Application of ethanol extract of caulis et folium wilsonii and dictamnine in pesticide - Google Patents
Application of ethanol extract of caulis et folium wilsonii and dictamnine in pesticide Download PDFInfo
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- CN114762503A CN114762503A CN202110051498.4A CN202110051498A CN114762503A CN 114762503 A CN114762503 A CN 114762503A CN 202110051498 A CN202110051498 A CN 202110051498A CN 114762503 A CN114762503 A CN 114762503A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention relates to an application of an ethanol extract of red-blood-white-leaf vine and dictamnine in pesticides. The ethanol extract of the red blood caulis Sinomenii and the Sinomenine can well inhibit tobacco mosaic virus; the ethanol extract of caulis et folium Hedyotis hemoglobin and cryptolepine can be used as bactericide, and has inhibitory activity on early blight of tomato, gibberellic disease of wheat, rice blast, phytophthora capsici, sclerotium of colza, gray mold of cucumber, rice sheath blight, cucumber wilt, peanut brown spot, apple ring rot, wheat sheath blight, corn microspore, watermelon anthrax and rice bakanae. The ethanol extract of herba Solani Lyrati and Sinomenine can be used as pesticide with insecticidal activity on aphid, diamondback moth, armyworm, corn borer and cotton bollworm.
Description
Technical Field
The invention relates to application of an ethanol extract of leucotaxillus hemoglobin and dictyosine in pesticides, in particular to application of the ethanol extract of leucotaxillus hemoglobin in plant virus resistance, plant source germ killing and agricultural pest killing, and belongs to the technical field of pesticides.
Background
The natural agricultural bioactive substance is a good guide of ecological pesticides, and the research and development process of agricultural agents can be accelerated by pertinently modifying the natural agricultural bioactive substance. Compared with the traditional chemical synthetic pesticide, the natural pesticide has the obvious advantages that: natural pesticides are generally more easily degraded in the environment; the compound has specificity to a target and is relatively safe to non-target organisms; the action mode is unique, and pests are difficult to generate drug resistance; compared with the chemical synthetic pesticide, the action mode of some natural pesticides is non-toxic and non-toxic, and the action modes comprise repelling, luring, food refusing, sterilization, growth and development regulation and the like. The above advantages make natural pesticides more and more interesting.
The white leafy vine plants are distributed in Asia and tropical regions of Africa, and China has two types of white leafy vines (Cryptolepis sinensis (Lour.) Merr) and ancient uncaria (Cryptolepis buchananii Roem. et Schult), which are produced in the south and southwest of China. Currently, the research on the plants of uncaria erythrostica, sargentgloryvine sanguinolentis and Cryptolepis triangularis is the most extensive in the world. The white vine plays an important role in the western non-traditional medicine, the root of the white vine is sold as a commodity, the leaves are used for treating malaria or are ground into powder to treat skin scratch and cut wounds, the water extract of the white vine is used for treating jaundice and hepatitis, and the immersion liquid of the root is also used for treating gastrointestinal dysfunction. In Canner, the water decoction of dried radix Sinomenii is used for treating fever caused by malaria, urinary tract and upper respiratory tract infection, rheumatism and venereal disease, etc., and the water extract of radix Sinomenii is used for treating amebic dysentery and as bitter stomachic in Congo traditional medicine. Its root is used in Nigeria for treating angina, rheumatoid disease and urinary and reproductive system infection. According to the compendium of Xinhua materia medica, the caulis spatholobi has the functions of clearing away heat and toxic material, dissipating blood stasis and relieving pain, is used for treating symptoms such as lung heat hemoptysis, gastric ulcer bleeding and the like, and is externally used for treating traumatic injuries, knife wounds, sores, scabies, poisonous insects and snake bites. Kara Phyto Riker pharmaceutical company developed white leaf vine bagged herbal tea for at least 20 years to treat malaria.
The main component of the indoloquinoline alkaloid rich in the solanum lyratum plant is solanine (cryptolepine). The name Cryptolepine was first proposed by Clinquart, 1929, who isolated a purple compound from Cryptolepis triangularis, a plant collected from Congo belgium, and he named this compound Cryptolepine. Research shows that the leucophylline and its analogs have pharmaceutical activity, including significant antimalarial activity, antitumor, meningitis, antimicrobial, antibacterial, anti-inflammatory, hypertension treating, noradrenaline receptor inhibiting, and antithrombotic activities.
Disclosure of Invention
The invention aims to provide application of an ethanol extract of the red fevervine leaves and the dictamnine in pesticides, and relates to application in the aspects of resisting plant viruses, killing plant source germs and killing agricultural pests.
The method for extracting the ethanol extract and the solanine of the red blood alburene comprises the following steps: 1.10kg of dried root of Kadsura longipedunculata (Cryptolepis sanguinolenta) was pulverized, extracted in 2.5L of ethanol for 7 days, and then filtered to collect the filtrate. The extraction of the filter residue is continued as above and repeated two or more times. Collecting all filtrates, and vacuum concentrating to obtain black paste, i.e. ethanol extract of caulis Seu folium Schisandrae Henryi. 10g of ethanol extract of the red vine, 10mL of dilute hydrochloric acid (1mol/L) is added, ultrasonic treatment is carried out for 20 minutes, and then chloroform (40mL) is used for extraction. The resulting aqueous phase was adjusted to pH 10 with aqueous ammonia and then extracted with chloroform (30 mL. times.3). Mixing organic phases, drying with anhydrous sodium sulfate, concentrating to obtain total alkaloids, adding HCl 3/MeOH/NH3(8: 2: 0.5) as eluent (R)f0.47) carrying out column chromatography to obtain the cryptolepine.
The ethanol extract of the red blood leucocyte rattan and the albutine provided by the invention have better activity of resisting plant viruses, killing plant source germs and killing agricultural pests.
The solarium album provided by the invention has an inhibition rate of more than 40% to tobacco mosaic virus under the concentration of 500mg/L, and is better than that of a commercial antiviral agent ribavirin.
The red blood white leaf vine ethanol extract and the cryptolepine provided by the invention have inhibition effects on tomato early blight, wheat scab, rice blast, phytophthora capsici, rape sclerotium, cucumber gray mold, rice sheath blight, cucumber wilt, peanut brown spot, apple ring rot, wheat sheath blight, corn microspots, watermelon anthracnose and rice bakanae plant-derived pathogen.
The red-blood-white-leaf-vine ethanol extract and the cryptolepine provided by the invention have insecticidal activity on aphids, diamondback moths, armyworms, corn borers and cotton bollworms.
Detailed Description
The following examples and biological test results can be used to further illustrate the invention, but are not meant to limit the invention.
Example 1: anti-tobacco mosaic Virus Activity
The measurement procedure was as follows
Virus purification and concentration determination:
virus purification and concentration determinations were performed in accordance with the tobamovirus SOP specifications compiled by the institute of elements institute of south-opening university. Centrifuging the virus crude extract with polyethylene glycol for 2 times, measuring concentration, and refrigerating at 4 deg.C for use.
Compound solution preparation:
weighing, adding dimethyl formamide to dissolve to obtain 1 × 10 solution5Diluting the mother liquor in mg/L with aqueous solution containing 1 ‰ Tween 80 to desired concentration; the ribavirin preparation is directly diluted by water.
In vitro effect:
the Shanxi tobacco leaves with the proper age are inoculated by rubbing and washed by running water, and the virus concentration is 10 mg/L. Cutting off after drying, cutting along the vein of the leaf, soaking the left and right half leaves in 1 ‰ of expectorant water and medicinal preparation respectively, taking out after 30min, performing moisture-keeping culture at suitable illumination temperature, repeating for 1 time and 3 times for each 3 leaves. And recording the number of the disease spots after 3d, and calculating the prevention effect.
The protection effect of the living body is as follows:
selecting 3-5 leaf-period Saxisi tobacco with uniform growth, spraying the whole plant, repeating for 3 times, and setting 1 ‰ Tween 80 aqueous solution as control. After 24h, the leaf surfaces are scattered with carborundum (500 meshes), the virus liquid is dipped by a writing brush, the whole leaf surfaces are lightly wiped for 2 times along the branch vein direction, the lower parts of the leaf surfaces are supported by palms, the virus concentration is 10mg/L, and the inoculated leaf surfaces are washed by running water. And recording the number of the disease spots after 3d, and calculating the prevention effect.
Therapeutic action in vivo:
selecting 3-5 leaf-stage Saxismoke with uniform growth vigor, inoculating virus with whole leaf of writing brush at a virus concentration of 10mg/L, and washing with running water after inoculation. After the leaves are harvested, the whole plant is sprayed with the pesticide, the treatment is repeated for 3 times, and a 1 per mill tween 80 aqueous solution is set for comparison. And 3d, recording the number of the scabs and calculating the control effect.
Inactivation of living body
Selecting 3-5 leaf-period Saxismoke with uniform growth, mixing the preparation with virus juice of the same volume, inactivating for 30min, performing friction inoculation with virus concentration of 20mg/L, washing with running water after inoculation, repeating for 3 times, and setting Tween 80 water solution of 1 ‰. The number of lesions after 3d was counted and the result was calculated.
Inhibition (%) < percent [ (control number of scorched spots-number of treated scorched spots)/control number of scorched spots ]. times.100%
The test results of the tobacco mosaic virus resisting activity of the ethanol extract of the red vine leaves and the dictamnine are as follows:
TABLE 1. ethanol extract of Leucothora hemoglobin and the activity of leupeptin against tobacco mosaic virus
It can be seen from Table 1 that the activity of picrinine against tobacco mosaic virus at a concentration of 500mg/L is better than that of the commercial antiviral agent ribavirin.
Example 2: activity for killing plant source germs
The measurement procedure is as follows
Taking tomato early blight as an example, the in vitro test method can be replaced by other in vitro test methods: inoculating the tomato early blight to PDA culture medium, culturing for 7 days, preparing bacterial dish with diameter of 4cm at the colony edge with a puncher, inoculating to PDA culture medium containing 50mg/L and no medicine, culturing for 4 days, measuring colony diameter, and comparing with control to calculate the inhibition percentage of the medicine.
The bactericidal activity test results of the ethanol extract of the red vine leaves and the biological total alkali of the white vine leaves are as follows:
TABLE 2 botanical pathogen killing activity (50mg/L) of ethanol extract of Haemoglobin vine and Sinomenine
From Table 2, it can be seen that 50mg/L of the ethanol extract of the red vine leaves and the total alkaloids of the white vine leaves have inhibition effects on early blight of tomato, gibberellic disease of wheat, rice blast, phytophthora capsici, sclerotium of colza, botrytis cinerea, rhizoctonia solani, fusarium wilt of cucumber, brown spot of peanut, ring spot of apple, wheat grain wither, corn speck, watermelon anthracnose and rice bakanae, wherein the inhibition rate of 50mg/L of the total alkaloids of the red vine leaves to sclerotium of colza, botrytis cinerea, brown spot of peanut, ring spot of apple and wheat grain wither is more than 80%.
Example 3: activity against agricultural pests
Activity assay of aphids
The aphid killing activity determination steps are as follows:
the test insects are aphids and normal groups raised by the broad bean leaves in a laboratory. Weighing the medicine, adding 1mL of dimethylformamide for dissolving, adding two drops of Tween-80 emulsifier, adding a certain amount of distilled water, and stirring uniformly to prepare the liquid medicine with the required concentration. Soaking the leaves of broad beans with aphids (about 60) in the medicament for 5 seconds, taking out and lightly drying, sucking the redundant medicament with filter paper, then inserting the branches of the broad beans into water-absorbing sponge, covering the branches with glass covers, sealing with gauze, checking the result for 96 hours, and repeating the steps for 3 times for each compound. The control was prepared by adding the emulsifier and solvent to distilled water and stirring the mixture uniformly.
Activity test of diamondback moth larvae
The leaf dipping method proposed by the International resistance action Committee was used. 2mg of the drug sample was weighed into a 10mL beaker on an analytical balance, dissolved in 50. mu.L of dimethylformamide (analytical grade), and added with 10mL of water to prepare 200ppm of the drug solution. Dipping the cabbage leaves with straight-head ophthalmological forceps for 2-3 seconds, and throwing off residual liquid. 1 tablet at a time, 3 tablets per sample. And sequentially placing the samples on the processing paper according to the sample marking sequence. After the liquid medicine is dried, the liquid medicine is put into a straight pipe with the length of 10 cm and provided with a mark, 2-year-old plutella xylostella larvae are inoculated, and the pipe orifice is covered by gauze. The experimental treatments were placed in a standard treatment chamber and the results checked after 96 h. Each compound was repeated 3 times. The control was prepared by adding the emulsifier and solvent to distilled water and stirring the mixture uniformly.
Activity test of armyworm
The experimental method of the armyworm comprises the following steps: leaf soaking. After the required concentration is prepared, soaking leaves with the diameter of about 5-6cm into the liquid medicine for 5-6 seconds, taking out, putting on absorbent paper for airing, putting in a designated culture dish, inoculating 10-head 3-year larvae, putting in an insect-raising room at 27 +/-1 ℃ for observing for 3-4 days, and then checking the result.
Activity test of corn borer
The experimental method of the corn borer comprises the following steps: the leaf soaking method comprises soaking leaf with diameter of 5-6cm in the medicinal liquid for 5-6 s after the required concentration is set, taking out, air drying on absorbent paper, placing in a designated culture dish, inoculating 10-head 3-year larva, placing in insect-raising room at 27 + -1 deg.C, observing for 3-4 days, and inspecting the result.
Activity test of bollworm
The experimental method of the cotton bollworm comprises the following steps: the feed mixing method is a method in which 3mL of the solution is transferred from the prepared solution and added to about 27g of the freshly prepared feed to obtain a desired concentration of ten times the dilution. The preparation is uniformly mixed and poured into a clean 24-hole plate, then is inoculated into 24-head three-year-old cotton bollworms after being cooled, and the inspection result is observed after 3 to 4 days.
The test results of the activity of the ethanol extract of the red-blood albefaction vine and the albefaction on aphids, diamond back moths, armyworms, corn borers and cotton bollworms are as follows:
TABLE 3 test insect mortality (%)
From table 3, it can be seen that the ethanol extract of red blood caulis Sinomenii and the cryptophylline have the effect of killing aphids, diamondback moths, armyworms, corn borers and cotton bollworms, wherein the killing activity of the cryptophylline on the diamondback moths at the concentration of 600mg/L is 85%, and the killing activity of the ethanol extract of red blood caulis Sinomenii at the concentration of 600mg/L is 100%.
Claims (6)
1. The application of ethanol extract of caulis et folium Hedyotis hemoglobin and Sinomenine in pesticide is provided.
2. The application of the ethanol extract of Corynia sanguinea Hance and the Sinomenine in pesticides as claimed in claim 1, wherein the anti-plant virus activity of the ethanol extract of Corynia sanguinea Hance and the Sinomenine can well inhibit tobacco mosaic virus.
3. The application of the ethanol extract of red blood muscadine and the cryptolepine in the pesticide as claimed in claim 1, wherein the ethanol extract of red blood muscadine and the cryptolepine are used as the bactericide and have good inhibitory activity against early blight of tomato, gibberellic disease of wheat, rice blast, phytophthora capsici, sclerotia napellus, cucumber gray mold, rice sheath blight, cucumber wilt, peanut brown spot, apple ring rot, wheat sheath blight, corn speck, watermelon anthrax and bakanae disease of rice.
4. The application of the ethanol extract of the red blood muscadine and the cryptolepine in the pesticide as claimed in claim 1, wherein the ethanol extract of the red blood muscadine and the cryptolepine are used as pesticides, and have insecticidal activity on agricultural pests such as aphids, diamond back moths, armyworms, corn borers and cotton bollworms.
5. The application of the ethanol extract of the red blood muscadine and the solanine on the pesticide as claimed in claim 1, characterized in that the ethanol extract of the red blood muscadine and the solanine can be respectively and directly used for preventing and controlling agricultural diseases.
6. The application of the ethanol extract of Corynia sanguinea Hance and the Sinomenine in pesticides as claimed in claim 1, wherein the ethanol extract of Corynia sanguinea Hance and the Sinomenine can be made into a compound agent with other pesticides for preventing and treating agricultural diseases.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110051498.4A CN114762503B (en) | 2021-01-15 | 2021-01-15 | Application of sargentgloryvine stem ethanol extract and sinomenine in pesticides |
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| CN202110051498.4A CN114762503B (en) | 2021-01-15 | 2021-01-15 | Application of sargentgloryvine stem ethanol extract and sinomenine in pesticides |
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| CN114762503A true CN114762503A (en) | 2022-07-19 |
| CN114762503B CN114762503B (en) | 2023-04-28 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996010015A1 (en) * | 1994-09-28 | 1996-04-04 | Shaman Pharmaceuticals, Inc. | Cryptolepine analogs with hypoglycemic activity |
| CN112106779A (en) * | 2019-06-20 | 2020-12-22 | 兰州大学 | Application of A-ring modified cryptolepine derivative in prevention and treatment of agricultural plant diseases |
-
2021
- 2021-01-15 CN CN202110051498.4A patent/CN114762503B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996010015A1 (en) * | 1994-09-28 | 1996-04-04 | Shaman Pharmaceuticals, Inc. | Cryptolepine analogs with hypoglycemic activity |
| CN112106779A (en) * | 2019-06-20 | 2020-12-22 | 兰州大学 | Application of A-ring modified cryptolepine derivative in prevention and treatment of agricultural plant diseases |
Non-Patent Citations (1)
| Title |
|---|
| 张璐 等: "白叶藤属植物化学成分、药理作用及毒理研究新进展", 《中南药学》 * |
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