CN114762503B - Application of sargentgloryvine stem ethanol extract and sinomenine in pesticides - Google Patents
Application of sargentgloryvine stem ethanol extract and sinomenine in pesticides Download PDFInfo
- Publication number
- CN114762503B CN114762503B CN202110051498.4A CN202110051498A CN114762503B CN 114762503 B CN114762503 B CN 114762503B CN 202110051498 A CN202110051498 A CN 202110051498A CN 114762503 B CN114762503 B CN 114762503B
- Authority
- CN
- China
- Prior art keywords
- ethanol extract
- sargentgloryvine stem
- white leaf
- vine
- ethanol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to an ethanol extract of sargentgloryvine stem and application of sargentgloryvine stem alkali in pesticides. The ethanol extract of the sargentgloryvine stem and the sinomenine can well inhibit tobacco mosaic virus; the white leaf vine ethanol extract and the white leaf vine alkali are used as bactericides and have inhibitory activity on tomato early blight, wheat gibberella, rice blast, phytophthora capsici, rape sclerotium, cucumber ash mold, rice sheath blight, cucumber wilt, peanut brown spots, apple sheath blight, wheat sheath blight, corn small spots, watermelon anthracnose and rice bakanae. The ethanol extract of the white leaf vine of the red blood and the white leaf vine of the white leaf vine are used as pesticides and have insecticidal activity on aphids, plutella xylostella, armyworms, corn borers and cotton bollworms.
Description
Technical Field
The invention relates to application of an ethanol extract of sargentgloryvine stem and white leaf rattan alkali in pesticides, in particular to application in the aspects of resisting plant viruses, killing plant source bacteria and killing agricultural pests, and belongs to the technical field of pesticides.
Background
The natural source agricultural bioactive substances are good leads of ecological pesticides, and the development process of agricultural agents can be accelerated by pertinently modifying the natural source agricultural bioactive substances. Compared with the traditional chemical synthesis pesticide, the natural source pesticide has obvious advantages: naturally derived pesticides are generally relatively degradable in the environment; the method has specificity to the target and is relatively safe to non-target organisms; the action mode is unique, and the pests are difficult to generate drug resistance; compared with chemical synthesized pesticides, the method has wider action modes, and certain natural source pesticides have non-toxic action modes, including repellency, attraction, antifeedant, sterilization, growth and development regulation and the like. The above advantages make natural source pesticides more and more interesting.
Plants of the genus white leaf vine are distributed in asia and tropical africa, and there are two species of white leaf vine (Cryptolepis sinensis (lour.) Merr.) and uncaria (Cryptolepis buchananii roem. Et Schult) produced in south and southwest of China. Currently, the most widely studied plants in the world are the ancient uncaria, white-leaf vine (Cryptolepis sanguinolenta) and Cryptolepis triangularis. The root of the white leaf vine plays an important role in Western medicine, and is sold as commodity, the leaf is used for treating malaria or is ground into powder for treating skin scratch and incised wound, the water extract of the white leaf vine is used for treating jaundice and hepatitis, and the immersion liquid of the root is also used for treating gastrointestinal dysfunction. In gana, the water decoction of dry white vine root is used in treating malaria, urinary tract and upper respiratory tract infection, rheumatism, venereal disease and other fever, and white She Tengshui extract is used in congo traditional medicine for treating amoebic dysentery and as bitter stomach strengthening medicine. Its root is used in nigeria for treating colic, rheumatoid and urinary and genital infections. The Xinhua Ben Cao gang Yu (New Chao Chan) records that the white leaf vine has the functions of clearing heat and detoxicating, removing stasis and relieving pain, is used for treating diseases such as lung heat hemoptysis, gastric ulcer hemorrhage and the like, and is externally used for treating traumatic injuries, sore and scabies, poisonous insect bite and snake bite. Bagged tea of the plant Saurus chinensis (Botrytis cinerea) developed by Gagnet-Riker pharmaceutical company has been used for at least 20 years to treat malaria.
Among the indoloquinoline alkaloids abundant in the plants of the sinomenium album, the most important component is sinomenine (cryptamine). The name Cryptolepine was originally proposed by Clinquat, which in 1929 isolated a purple compound from plant Cryptolepis triangularis collected from Congo, belgium, and which was named Cryptolepine. Studies have shown that phylline and its analogs have pharmaceutical activities including significant antimalarial activity, antitumor, anti-meningitis, antimicrobial, antibacterial, anti-inflammatory, hypertension treatment, norepinephrine receptor inhibition, antithrombotic, and like activities.
Disclosure of Invention
The invention aims to provide an ethanol extract of sargentgloryvine stem and application of sargentgloryvine stem in pesticides, and the application relates to the application in the aspects of resisting plant viruses, killing plant source bacteria and killing agricultural pests.
The method for extracting the ethanol extract of the sargentgloryvine stem and the white leaf rattan alkali comprises the following steps: 1.10kg of dried roots of sargentgloryvine stem (Cryptolepis sanguinolenta) are taken, crushed, leached in 2.5L of ethanol for 7 days, and then filtered, and the filtrate is collected. The filter residue is further leached as above and repeated two or more times. All the filtrate is collected and concentrated in vacuum, and finally the black paste is the ethanol extract of the white vine of red blood. 10g of ethanol extract of Hemioga febrifuga is taken, 10mL of diluted hydrochloric acid (1 mol/L) is added, ultrasonic treatment is carried out for 20 minutes, and chloroform extraction (40 mL) is carried out. The resulting aqueous phase was adjusted to pH 10 with ammonia and then extracted with chloroform (30 mL. Times.3)Taking. Mixing the organic phases, drying with anhydrous sodium sulfate, concentrating to obtain total alkaloids, and adding HCl 3 /MeOH/NH 3 (8:2:0.5) as eluent (R) f 0.47 Performing column chromatography to obtain the sinomenine.
The ethanol extract of the white leaf vine and the white leaf vine alkali provided by the invention have better anti-plant virus activity, plant source germ killing activity and agricultural pest killing activity.
The inhibition rate of the white leaf rattan alkali provided by the invention to the tobacco mosaic virus is more than 40% at the concentration of 500mg/L, and is better than that of the commercial antiviral agent ribavirin.
The white leaf vine ethanol extract and the white leaf vine alkaloid provided by the invention have inhibition effects on plant pathogens such as tomato early blight, wheat scab, rice blast, phytophthora capsici, rape sclerotium, cucumber gray mold, rice sheath blight, cucumber wilt, peanut brown spots, apple ring, wheat sheath blight, corn small spots, watermelon anthracnose and rice bakanae.
The ethanol extract of the white leaf vine and the white leaf vine alkali provided by the invention have insecticidal activity on aphids, plutella xylostella, armyworms, corn borers and cotton bollworms.
Detailed Description
The following examples and biological test results may be used to further illustrate the invention, but are not meant to limit the invention.
Example 1: anti-tobacco mosaic virus activity
The measurement procedure was as follows
Virus purification and concentration determination:
the virus purification and concentration measurement are carried out by compiling tobacco mosaic virus SOP standard according to a measuring room generated by elements of university of south China. After 2 times of polyethylene glycol centrifugation treatment, the concentration of the virus crude extract is measured, and the virus crude extract is refrigerated at 4 ℃ for standby.
Compound solution preparation:
after weighing, the raw materials are added with dimethylformamide for dissolution to prepare 1 multiplied by 10 5 mg/L mother liquor, and then diluting the mother liquor to the required concentration by using an aqueous solution containing 1 per mill of Tween 80; the ribavirin preparation is directly diluted with water.
Ex vivo action:
the Shanxi tobacco leaves with proper age are rubbed and washed by running water, and the virus concentration is 10mg/L. Cutting after harvesting, cutting along the middle vein of leaf, soaking left and right half leaves in 1 millwarm water and medicinal preparation respectively, taking out after 30min, and culturing under proper illumination temperature under moisture keeping condition, wherein every 3 leaves are 1 time and 3 times. And after 3d, recording the number of the lesions, and calculating the control effect.
Living body protecting action:
3-5 She Qishan Xiyan with uniform growth vigor is selected, the whole plant is sprayed and applied, each treatment is repeated for 3 times, and 1 permillage of Tween 80 aqueous solution is used for comparison. After 24h, the leaf surface is spread with silicon carbide (500 meshes), the whole leaf surface is dipped with a virus liquid by a writing brush, the whole leaf surface is lightly rubbed for 2 times along the branch pulse direction, the lower part of the leaf surface is supported by a palm, the virus concentration is 10mg/L, and the leaf surface is washed by running water after inoculation. And after 3d, recording the number of the lesions, and calculating the control effect.
In vivo therapeutic action:
3-5 She Qishan Xiyan with uniform growth vigor is selected, the whole leaf of the writing brush is inoculated with virus, the virus concentration is 10mg/L, and the whole leaf is washed by running water after inoculation. After leaf surface is dried, spraying and applying the whole plant, repeating for 3 times every treatment, and setting 1%Tween 80 water solution as a control. After 3d, the number of lesions is recorded, and the control effect is calculated.
In vivo passivation
Selecting 3-5 She Qishan Xie smoke with uniform growth vigor, mixing the medicament with an equal volume of virus juice, inactivating for 30min, performing friction inoculation, wherein the virus concentration is 20mg/L, washing with running water after inoculation, repeating for 3 times, and setting 1 milltween 80 water solution for comparison. And counting the number of lesions after 3d, and calculating a result.
Inhibition ratio (%) = [ (control number of dried spots-treated number of dried spots)/control number of dried spots ] ×100%
The results of the tobacco mosaic virus resistance activity test of the ethanol extract of the sargentgloryvine stem and the sinomenine are as follows:
TABLE 1 ethanol extract of Hedyotis Hedyotidis Diffusae and its activity against tobacco mosaic Virus
As can be seen from Table 1, the activity of the sinomenine against tobacco mosaic virus at a concentration of 500mg/L is better than that of the commercially available antiviral agent ribavirin.
Example 2: activity of killing plant source germ
The measurement procedure was as follows
Taking tomato early blight as an example, the method can be replaced by other in-vitro testing methods: the tomato early blight bacteria are inoculated on a PDA culture medium for 7 days, bacterial dishes with the diameter of 4cm are prepared at the edge of the bacterial colonies by using a puncher, inoculated on the PDA culture medium containing 50mg/L and no medicament for 4 days for culture, the bacterial colony diameter is measured, and the inhibition percentage of the medicament is calculated compared with a control.
The bactericidal activity test results of the ethanol extract of the sarcandra glabra and the total alkaloids of the sarcandra glabra are as follows:
TABLE 2 phytopathogenic Activity of ethanol extract of Hedyotis rupestris and Aleurodine (50 mg/L)
From Table 2, it can be seen that the ethanol extract of white leaf vine and the total alkaloids of white leaf vine have inhibitory effect on early blight of tomato, gibberella wheat, rice blast, phytophthora capsici, sclerotium of rape, botrytis cinerea, banded sclerotial blight of rice, wilt of cucumber, brown spot of peanut, ring spot of apple, banded sclerotium of wheat, small spot of corn, anthrax of watermelon and bakanae of rice at 50mg/L, wherein the inhibitory rate of the white leaf vine base on sclerotium of rape, gray mold of cucumber, brown spot of peanut, ring spot of apple and ring spot of wheat at 50mg/L is more than 80%.
Example 3: agricultural pest killing activity
Aphid Activity test
The aphid killing activity is determined as follows:
the test insects are aphids and are normal populations raised by laboratory broad bean leaves. Weighing the medicine, adding 1mL of dimethylformamide for dissolution, adding two drops of Tween-80 emulsifier, adding a certain amount of distilled water, and stirring uniformly to prepare the medicine liquid with the required concentration. The leaf of broad bean with aphid (about 60) was immersed in the preparation for 5 seconds, removed and dried gently, the excess preparation was blotted with filter paper, then the broad bean shoots were inserted into the blotted sponge and covered with glass cover, sealed with gauze, and the results were checked for 96 hours and each compound was repeated 3 times. The control was prepared by adding only the emulsifier and solvent to distilled water and stirring well.
Plutella xylostella larva activity test
Leaf dipping proposed by the International Commission on resistance action was used. 2mg of the drug sample was weighed on an analytical balance in a 10mL small beaker, 50. Mu.L of dimethylformamide (analytically pure) was added for dissolution, and 10mL of water was added to prepare a 200ppm drug solution. The cabbage leaves are immersed in a straight ophthalmic tweezer for 2-3 seconds, and the residual liquid is thrown away. 1 tablet at a time, 3 tablets per sample. Sequentially placed on the treated paper in the order of sample marking. After the liquid medicine is dried, the liquid medicine is put into a straight tube with the length of 10em and with marks, 2-year-old plutella xylostella larvae are inoculated, and the mouth of the tube is covered by gauze. The experimental treatment was placed in a standard treatment chamber and the results were checked after 96 h. Each compound was repeated 3 times. The control was prepared by adding only the emulsifier and solvent to distilled water and stirring well.
Activity test of armyworm
The experiment method of the armyworm comprises the following steps: leaf soaking method. After the required concentration is prepared, the leaves with the diameter of about 5-6cm are immersed in the liquid medicine for 5-6 seconds, taken out, put on absorbent paper for airing, put in a designated culture dish, inoculated with 10 larvae of 3 ages, put in an insect-breeding room with the temperature of 27+/-1 ℃ for observation for 3-4 days, and then the result is checked.
Activity test of corn borer
The experimental method of corn borers comprises the following steps: immersing leaves with the required concentration after configuration in the liquid medicine for 5-6 seconds, taking out, airing on absorbent paper, placing in a designated culture dish, inoculating 10 larvae with 3 ages, placing in an insect-raising room with 27+/-1 ℃ for observing for 3-4 days, and checking results.
Activity test of bollworm
Experimental method of cotton bollworms: in the feed compounding method, 3mL of the prepared solution was removed and added to about 27g of the freshly prepared feed, thereby obtaining a desired concentration ten times as much as dilution. Uniformly mixing the medicines, pouring the medicines into a clean 24-hole plate, airing, inoculating 24-head three-year-old cotton bollworms, and observing the inspection result after 3-4 days.
The results of the activity test of the ethanol extract of the sargentgloryvine stem and the white leaf rattan on aphids, plutella xylostella, armyworms, corn borers and cotton bollworms are as follows:
TABLE 3 mortality of test insects at various concentrations (%)
From Table 3, it can be seen that the ethanol extract of Hemium globosum and the white leaf rattan alkali have killing effect on aphids, plutella xylostella, armyworms, corn borers and cotton bollworms, wherein the killing activity of the white leaf rattan alkali on plutella xylostella at 600mg/L concentration is 85%, and the killing activity of the ethanol extract of Hemium globosum at 600mg/L concentration on plutella xylostella is 100%.
Claims (4)
1. The application of the ethanol extract of the sargentgloryvine stem and/or the sinomenine in inhibiting the tobacco mosaic virus is characterized in that the ethanol extract of the sargentgloryvine stem is extracted by the following steps: taking 1.10kg of dried roots of the sargentgloryvine stem (Cryptolepis sanguinolenta), crushing, leaching in 2.5L of ethanol for 7 days, filtering, collecting filtrate, continuously leaching filter residues by the method, repeating the steps for two or more times, collecting all the filtrate, and concentrating in vacuum to finally obtain black paste, namely the sargentgloryvine stem ethanol extract.
2. The application of the ethanol extract of the sargentgloryvine stem and/or the white leaf rattan alkali in preparing pesticide bactericides is characterized in that the fungus is a pathogenic fungus of early blight of tomatoes, rice blast, apple ring, small corn spots, watermelon anthrax or rice bakanae, and the extraction method of the ethanol extract of the sargentgloryvine stem is as follows: taking 1.10kg of dried roots of the sargentgloryvine stem (Cryptolepis sanguinolenta), crushing, leaching in 2.5L of ethanol for 7 days, filtering, collecting filtrate, continuously leaching filter residues by the method, repeating the steps for two or more times, collecting all the filtrate, and concentrating in vacuum to finally obtain black paste, namely the sargentgloryvine stem ethanol extract.
3. The application of the white leaf vine ethanol extract and/or the white leaf vine alkaloid in preparing pesticide is characterized in that the insect is aphid, plutella xylostella, armyworm, corn borer or cotton bollworm, and the white leaf vine ethanol extract extraction method comprises the following steps: taking 1.10kg of dried roots of the sargentgloryvine stem (Cryptolepis sanguinolenta), crushing, leaching in 2.5L of ethanol for 7 days, filtering, collecting filtrate, continuously leaching filter residues by the method, repeating the steps for two or more times, collecting all the filtrate, and concentrating in vacuum to finally obtain black paste, namely the sargentgloryvine stem ethanol extract.
4. The use according to any one of claims 1 to 3, wherein the ethanol extract of sargentgloryvine stem and/or the sinomenine is formulated with other pesticides.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110051498.4A CN114762503B (en) | 2021-01-15 | 2021-01-15 | Application of sargentgloryvine stem ethanol extract and sinomenine in pesticides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110051498.4A CN114762503B (en) | 2021-01-15 | 2021-01-15 | Application of sargentgloryvine stem ethanol extract and sinomenine in pesticides |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114762503A CN114762503A (en) | 2022-07-19 |
| CN114762503B true CN114762503B (en) | 2023-04-28 |
Family
ID=82363822
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110051498.4A Active CN114762503B (en) | 2021-01-15 | 2021-01-15 | Application of sargentgloryvine stem ethanol extract and sinomenine in pesticides |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114762503B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996010015A1 (en) * | 1994-09-28 | 1996-04-04 | Shaman Pharmaceuticals, Inc. | Cryptolepine analogs with hypoglycemic activity |
| CN112106779A (en) * | 2019-06-20 | 2020-12-22 | 兰州大学 | Application of A-ring modified cryptolepine derivative in prevention and treatment of agricultural plant diseases |
-
2021
- 2021-01-15 CN CN202110051498.4A patent/CN114762503B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996010015A1 (en) * | 1994-09-28 | 1996-04-04 | Shaman Pharmaceuticals, Inc. | Cryptolepine analogs with hypoglycemic activity |
| CN112106779A (en) * | 2019-06-20 | 2020-12-22 | 兰州大学 | Application of A-ring modified cryptolepine derivative in prevention and treatment of agricultural plant diseases |
Non-Patent Citations (1)
| Title |
|---|
| 白叶藤属植物化学成分、药理作用及毒理研究新进展;张璐 等;《中南药学》;第18卷(第2期);第265-269页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114762503A (en) | 2022-07-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Njoku et al. | Phytochemical constituents of some selected medicinal plants | |
| Shaheen et al. | In vitro and in vivo activity of Peganum harmala L. alkaloids against phytopathogenic bacteria | |
| CN103999884B (en) | A kind ofly prevent and treat biologic product of Radix Astragali powdery mildew and preparation method thereof | |
| CN107573392B (en) | Glycosyl-substituted genipin derivative and preparation and application thereof | |
| CN112079692B (en) | Compound for preventing and treating plant pathogenic bacteria and application thereof | |
| CN104920518A (en) | Traditional Chinese medicine disinfection spray and preparation method thereof | |
| CN104367907B (en) | A kind of Chinese herbal medicine sheep tick insect resistant agent and its preparation and application method | |
| CN112209948A (en) | Aristolochic acid derivative and application thereof | |
| Amabye | Evaluation of phytochemical, chemical composition, antioxidant and antimicrobial screening parameters of Rhamnus prinoides (Gesho) available in the market of Mekelle, Tigray, Ethiopia | |
| Njoku et al. | Phytochemical constituents of some selected medicinal plants | |
| CN114478521B (en) | Quinolone-lixidine alkaloid and preparation method and application thereof | |
| CN114762503B (en) | Application of sargentgloryvine stem ethanol extract and sinomenine in pesticides | |
| Tiwari et al. | Inhibitory effect of cow urine based plant extracts against Rhizoctonia solani causing sheath blight of rice | |
| CN113016805B (en) | Application of phthalide derivatives in preventing and treating plant virus, killing bacteria, killing insects and killing mites | |
| CN109422744B (en) | Matrine derivative, synthesis thereof and application thereof in prevention and treatment of plant diseases and insect pests | |
| CN108651465A (en) | A kind of iridoid glycoside is in the application for preparing pesticide | |
| CN116283987A (en) | Matrine alkaloid and preparation method and application thereof | |
| CN108785374A (en) | Faint scent cassia bark skin bacteriostatic gel and preparation method thereof | |
| Bakr | Changes of hemoglobin content and glucose levels in the blood of Rattus norvegicus by water extracts of Azadirachta indica | |
| CN110074145B (en) | Application of michelia figo ethanol extract in pesticide | |
| CN113321655A (en) | Preparation and application of evodia rutaecarpa botanical insecticide | |
| KR101633003B1 (en) | Composition for controlling nematodes comprising an extract of Melochia species, and method for controlling nematodes using the same | |
| CN106867674A (en) | A kind of extraction purification of bean curd dish volatile oil and detection method and its application | |
| Yamaguchi et al. | AABOMYCIN A, A NEW ANTIBIOTIC. II BIOLOGICAL STUDIES ON AABOMYCIN A | |
| CN105638662B (en) | Curculigoside is applied in insecticide is prepared |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |