CN114761554A - 具有n-酰化活性的修饰酶 - Google Patents

具有n-酰化活性的修饰酶 Download PDF

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Publication number
CN114761554A
CN114761554A CN202080070926.1A CN202080070926A CN114761554A CN 114761554 A CN114761554 A CN 114761554A CN 202080070926 A CN202080070926 A CN 202080070926A CN 114761554 A CN114761554 A CN 114761554A
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China
Prior art keywords
amino acid
acyl
acid sequence
acid
amino
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Pending
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CN202080070926.1A
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English (en)
Chinese (zh)
Inventor
永田秀美
田上宇乃
野崎博之
肥沼景子
D·斯特雷尔特索瓦
A·赫鲁谢瓦
J·卡塔什基纳
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Ajinomoto Co Inc
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Ajinomoto Co Inc
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Publication of CN114761554A publication Critical patent/CN114761554A/zh
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/20—Aspartic acid; Asparagine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C12Y301/02014—Oleoyl-[acyl-carrier-protein] hydrolase (3.1.2.14), i.e. ACP-thioesterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y602/00—Ligases forming carbon-sulfur bonds (6.2)
    • C12Y602/01—Acid-Thiol Ligases (6.2.1)
    • C12Y602/01003—Long-chain-fatty-acid-CoA ligase (6.2.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/02—Acid—amino-acid ligases (peptide synthases)(6.3.2)

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  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202080070926.1A 2019-10-10 2020-10-09 具有n-酰化活性的修饰酶 Pending CN114761554A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
RU2019131887 2019-10-10
RU2019131887 2019-10-10
PCT/JP2020/038340 WO2021070942A1 (ja) 2019-10-10 2020-10-09 Ｎ－アシル化活性を有する改変酵素

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CN114761554A true CN114761554A (zh) 2022-07-15

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US (1) US12545935B1 (https=)
EP (1) EP4043578A4 (https=)
JP (2) JPWO2021070942A1 (https=)
CN (1) CN114761554A (https=)
WO (1) WO2021070942A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113481223A (zh) * 2021-08-12 2021-10-08 广东省禾基生物科技有限公司 一种重组酰胺水解酶基因及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0622776A (ja) * 1992-07-09 1994-02-01 C C I Kk 酵素法によるｎ−長鎖アシルアミノ酸の製造方法
US20180127791A1 (en) * 2015-04-14 2018-05-10 Evonik Degussa Gmbh Acyl amino acid production
JP2018085969A (ja) * 2016-11-29 2018-06-07 公立大学法人 富山県立大学 Ｎα−アシル−Ｌ−アミノ酸の製造方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7981685B2 (en) 2007-04-16 2011-07-19 Modular Genetics, Inc. Generation of acyl amino acids
BR112015015055A2 (pt) * 2012-12-21 2017-10-03 Pioneer Hi Bred Int Método para desintoxicar um herbicida análogo de auxina, método para controlar pelo menos uma erva em uma área de cultivo, método para testar uma resposta de planta a um ou mais compostos
EP2843043A1 (en) 2013-08-27 2015-03-04 Evonik Industries AG A method for producing acyl amino acids
JP6924405B2 (ja) 2017-06-24 2021-08-25 株式会社ノーリツ 燃焼装置およびこれを備えた給湯装置
JP7306379B2 (ja) * 2018-04-13 2023-07-11 味の素株式会社 Ｎ－アシル－アミノ基含有化合物の製造方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0622776A (ja) * 1992-07-09 1994-02-01 C C I Kk 酵素法によるｎ−長鎖アシルアミノ酸の製造方法
US20180127791A1 (en) * 2015-04-14 2018-05-10 Evonik Degussa Gmbh Acyl amino acid production
JP2018085969A (ja) * 2016-11-29 2018-06-07 公立大学法人 富山県立大学 Ｎα−アシル−Ｌ−アミノ酸の製造方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
EIKO WADA等: "Enzymatic synthesis of N‐acyl‐l‐amino acids in a glycerol‐water system using acylase I from pig kidney", JOURNAL OF THE AMERICAN OIL CHEMISTS\' SOCIETY, vol. 79, no. 1, 1 January 2002 (2002-01-01), pages 41 - 46 *
TABATA, S.等: "Auxin-responsive GH3 family protein [Arabidopsis thaliana]", pages 1 - 3, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/protein/15239653?sat=51&satkey=36856938> *
佚名: "PREDICTED: indole-3-acetic acid-amido synthetase GH3.6 [Brassica rapa]", pages 1 - 2, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/protein/685272049?sat=46&satkey=38478225> *

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US12545935B1 (en) 2026-02-10
JP2025131708A (ja) 2025-09-09
JPWO2021070942A1 (https=) 2021-04-15
WO2021070942A1 (ja) 2021-04-15
EP4043578A1 (en) 2022-08-17
EP4043578A4 (en) 2024-08-21

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