JPWO2021070942A1 - - Google Patents

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Publication number
JPWO2021070942A1
JPWO2021070942A1 JP2021551725A JP2021551725A JPWO2021070942A1 JP WO2021070942 A1 JPWO2021070942 A1 JP WO2021070942A1 JP 2021551725 A JP2021551725 A JP 2021551725A JP 2021551725 A JP2021551725 A JP 2021551725A JP WO2021070942 A1 JPWO2021070942 A1 JP WO2021070942A1
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JP
Japan
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2021551725A
Other languages
Japanese (ja)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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Publication of JPWO2021070942A1 publication Critical patent/JPWO2021070942A1/ja
Priority to JP2025093225A priority Critical patent/JP2025131708A/ja
Pending legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1025Acyltransferases (2.3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1025Acyltransferases (2.3)
    • C12N9/104Aminoacyltransferases (2.3.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93Ligases (6)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/02Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/04Alpha- or beta- amino acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/04Alpha- or beta- amino acids
    • C12P13/20Aspartic acid; Asparagine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436Fatty acid esters
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/02Thioester hydrolases (3.1.2)
    • C12Y301/02014Oleoyl-[acyl-carrier-protein] hydrolase (3.1.2.14), i.e. ACP-thioesterase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y602/00Ligases forming carbon-sulfur bonds (6.2)
    • C12Y602/01Acid-Thiol Ligases (6.2.1)
    • C12Y602/01003Long-chain-fatty-acid-CoA ligase (6.2.1.3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y603/00Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/02Acid—amino-acid ligases (peptide synthases)(6.3.2)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2021551725A 2019-10-10 2020-10-09 Pending JPWO2021070942A1 (https=)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025093225A JP2025131708A (ja) 2019-10-10 2025-06-04 N-アシル化活性を有する改変酵素

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
RU2019131887 2019-10-10
PCT/JP2020/038340 WO2021070942A1 (ja) 2019-10-10 2020-10-09 N-アシル化活性を有する改変酵素

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2025093225A Division JP2025131708A (ja) 2019-10-10 2025-06-04 N-アシル化活性を有する改変酵素

Publications (1)

Publication Number Publication Date
JPWO2021070942A1 true JPWO2021070942A1 (https=) 2021-04-15

Family

ID=75436843

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2021551725A Pending JPWO2021070942A1 (https=) 2019-10-10 2020-10-09
JP2025093225A Pending JP2025131708A (ja) 2019-10-10 2025-06-04 N-アシル化活性を有する改変酵素

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2025093225A Pending JP2025131708A (ja) 2019-10-10 2025-06-04 N-アシル化活性を有する改変酵素

Country Status (5)

Country Link
US (1) US12545935B1 (https=)
EP (1) EP4043578A4 (https=)
JP (2) JPWO2021070942A1 (https=)
CN (1) CN114761554A (https=)
WO (1) WO2021070942A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113481223A (zh) * 2021-08-12 2021-10-08 广东省禾基生物科技有限公司 一种重组酰胺水解酶基因及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0622776A (ja) * 1992-07-09 1994-02-01 C C I Kk 酵素法によるn−長鎖アシルアミノ酸の製造方法
US20150351390A1 (en) * 2012-12-21 2015-12-10 Pioneer Hi-Bred International, Inc. Compositions and methods for auxin-analog conjugation
US20180127791A1 (en) * 2015-04-14 2018-05-10 Evonik Degussa Gmbh Acyl amino acid production
JP2018085969A (ja) * 2016-11-29 2018-06-07 公立大学法人 富山県立大学 Nα−アシル−L−アミノ酸の製造方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7981685B2 (en) 2007-04-16 2011-07-19 Modular Genetics, Inc. Generation of acyl amino acids
EP2843043A1 (en) 2013-08-27 2015-03-04 Evonik Industries AG A method for producing acyl amino acids
JP6924405B2 (ja) 2017-06-24 2021-08-25 株式会社ノーリツ 燃焼装置およびこれを備えた給湯装置
JP7306379B2 (ja) * 2018-04-13 2023-07-11 味の素株式会社 N-アシル-アミノ基含有化合物の製造方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0622776A (ja) * 1992-07-09 1994-02-01 C C I Kk 酵素法によるn−長鎖アシルアミノ酸の製造方法
US20150351390A1 (en) * 2012-12-21 2015-12-10 Pioneer Hi-Bred International, Inc. Compositions and methods for auxin-analog conjugation
US20180127791A1 (en) * 2015-04-14 2018-05-10 Evonik Degussa Gmbh Acyl amino acid production
JP2018085969A (ja) * 2016-11-29 2018-06-07 公立大学法人 富山県立大学 Nα−アシル−L−アミノ酸の製造方法

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
"Modulating plant hormones by enzyme action: the GH3 family of acyl acid amido synthetases", PLANT SIGNAL. BEHAV., vol. 5, no. 12, JPN6019017243, 2010, pages 1607 - 1612, ISSN: 0005540284 *
ANALYTICAL BIOCHEMISTRY, vol. 390, JPN6024036483, 2009, pages 149 - 154, ISSN: 0005412296 *
JAOCS, vol. 79, no. 1, JPN6024036481, 2002, pages 41 - 46, ISSN: 0005412294 *
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 54, JPN6024036482, 2006, pages 72 - 78, ISSN: 0005412295 *
THE PLANT CELL, vol. 17, JPN6019017245, 2005, pages 616 - 627, ISSN: 0005412293 *

Also Published As

Publication number Publication date
US12545935B1 (en) 2026-02-10
JP2025131708A (ja) 2025-09-09
CN114761554A (zh) 2022-07-15
WO2021070942A1 (ja) 2021-04-15
EP4043578A1 (en) 2022-08-17
EP4043578A4 (en) 2024-08-21

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