CN114747518B - Breeding method of Chlamys nobilis - Google Patents

Breeding method of Chlamys nobilis Download PDF

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Publication number
CN114747518B
CN114747518B CN202210306084.6A CN202210306084A CN114747518B CN 114747518 B CN114747518 B CN 114747518B CN 202210306084 A CN202210306084 A CN 202210306084A CN 114747518 B CN114747518 B CN 114747518B
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polylactic acid
cage
trace elements
culturing
parts
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CN114747518A (en
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林炎权
李秀娟
王康
林先
江琳
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Shenzhen Haichuang Marine Technology Co ltd
Shenzhen Taifeng Dongfang Ocean Biotechnology Co ltd
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Shenzhen Haichuang Marine Technology Co ltd
Shenzhen Taifeng Dongfang Ocean Biotechnology Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/50Culture of aquatic animals of shellfish
    • A01K61/54Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/50Culture of aquatic animals of shellfish
    • A01K61/54Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
    • A01K61/55Baskets therefor
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/22Compounds of alkali metals
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/24Compounds of alkaline earth metals, e.g. magnesium
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/30Oligoelements
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Inorganic Chemistry (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Insects & Arthropods (AREA)
  • Birds (AREA)
  • Farming Of Fish And Shellfish (AREA)
  • Manufacture Of Porous Articles, And Recovery And Treatment Of Waste Products (AREA)

Abstract

The invention discloses a method for culturing Chlamys nobilis, which comprises the steps of placing young scallops in a cage, culturing in seawater, wherein a net bag is fixed in the cage, and polylactic acid foam containing microelements is placed in the net bag; the polylactic acid foam containing the trace elements is prepared from the following raw materials in parts by mass: 100 parts of polylactic acid, 2-4 parts of nucleating agent and 1-3 parts of trace elements. The method can continuously provide trace elements in the scallop culturing process, improve the immunity of the scallop, reduce the occurrence rate and death rate of diseases, promote the growth of the scallop, and further improve the yield and quality of the Chlamys nobilis.

Description

Breeding method of Chlamys nobilis
Technical Field
The invention relates to the technical field of aquaculture, in particular to a method for culturing Chlamys nobilis.
Background
The Chlamys nobilis is mainly distributed in the eastern sea and the south sea of China, has the characteristics of rich nutrition, delicious taste and tender meat quality, has high growth speed and short cultivation period, can bring good economic benefit, and is one of main scallop cultivation types of China. At present, the artificial culture mode of the chlamys nobilis mainly comprises the step of carrying out cage-covering culture on the seedlings obtained by artificial seedling culture in a sea area.
In the culture process of the chlamys nobilis, the seawater environment plays a crucial role. In recent years, due to the increasing serious seawater ecological environment problems, the occurrence rate of diseases is increased, and the yield of the Chlamys nobilis is affected. The microelements are helpful for improving the immunity of the Chlamys nobilis, thereby reducing the disease rate and death rate to a certain extent. However, if trace elements are directly put in the sea area, the problems of uneven putting, overhigh local concentration and the like are easily caused, and manpower and material resources are consumed; when the microelements are added into artificial baits for feeding, the problems of unbalanced nutrition and water pollution of seawater can be caused.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a method for culturing Chlamys nobilis.
The invention is realized by the following technical scheme:
a method for culturing Chlamys nobilis comprises placing young scallops in a cage, culturing in seawater, wherein a mesh bag is fixed inside the cage, and polylactic acid foam containing microelements is placed in the mesh bag; the polylactic acid foam containing the trace elements is prepared from the following raw materials in parts by mass: 100 parts of polylactic acid, 2-4 parts of nucleating agent and 1-3 parts of trace elements, and the preparation method comprises the following steps: firstly, the raw materials are subjected to melt extrusion granulation, and then, the supercritical fluid foaming is carried out, so that the polylactic acid foam containing trace elements is prepared.
Preferably, the trace element is one or a mixture of a plurality of salts of Fe, mg, zn, se; preferably, the trace elements are selected from FeSO 4 、MgSO 4 、ZnSO 4 、Na 2 SeO 3 The mass ratio is (1-2): (1-2): (2-3): (5-10).
Preferably, the nucleating agent is one or a mixture of a plurality of montmorillonite, talcum powder and kaolin.
Preferably, the melt flow rate of the polylactic acid at 210 ℃/2.16kg is 3-8g/10min.
Preferably, the preparation method of the polylactic acid foam containing trace elements comprises the following steps:
(1) Uniformly mixing polylactic acid, a nucleating agent and trace elements, and then adding the mixture into a screw extruder for melt extrusion to obtain polylactic acid granules containing the trace elements;
(2) Placing the polylactic acid granules containing the trace elements into a high-pressure reaction kettle, and injecting supercritical CO 2 Swelling and penetrating for a certain time under a certain temperature and pressure, then rapidly decompressing and cooling to obtain the polylactic acid foam containing microelements.
Preferably, in step (1), melt extrusion is carried out at 160-200 ℃.
Preferably, in the step (2), swelling and infiltration are carried out for 15-30min at a temperature of 90-120 ℃ and a pressure of 20-25 MPa; in the step (2), the pressure release speed of the rapid pressure release is 20-100MPa/s.
Preferably, the sleeve cage comprises a cultivation cage and a sleeve net sleeved outside the cultivation cage, wherein the diameter of the cultivation cage is 30-40cm, the height of the cultivation cage is 1.2-1.5m, the mesh is 2-2.5cm, and the number of layers is 5-8; the mesh of the sleeve net is 1-1.5cm.
Preferably, in the cultivation cage, the cultivation density of the young shellfish is 20-30 per layer.
Preferably, the dosage of the polylactic acid foam containing microelements is 100-200 g/cage.
The invention has the advantages that:
in the cage culture process of the Chlamys nobilis, the polylactic acid foam containing trace elements is fixed in the cage for culture by using the net bag, and is prepared by carrying out supercritical fluid foaming under certain conditions after polylactic acid, a nucleating agent and trace elements are jointly melted and extruded, so that the comb-shaped scallop has a uniform micropore structure with communicated pore channels, has larger specific surface area, trace elements are uniformly distributed in micropore boundaries or the inside of the polylactic acid, and can be gradually contacted with seawater for dissolution and release through the communicated pore channels and degradation of the polylactic acid between the micropores, thereby effectively improving the concentration of the trace elements in the seawater. The invention can maintain the requirement of the Chlamys nobilis on the trace elements by a one-time throwing method, improve the immunity of the scallop and promote the growth without replacing for a plurality of times, has no risk of causing unbalanced seawater nutrition and water pollution compared with the direct throwing of trace elements or baits containing trace elements, saves manpower and material resources, and has good economic benefit.
Detailed Description
Example 1
A method for culturing Chlamys nobilis comprises placing young scallops in a cage, culturing in seawater, fixing mesh bag inside the cage, and placing polylactic acid foam containing microelements in the mesh bag with dosage of 100 g/cage; the cultivation cage comprises a cultivation cage and a cover net sleeved outside the cultivation cage, wherein the diameter of the cultivation cage is 30cm, the height of the cultivation cage is 1.2m, the mesh is 2cm, the number of layers is 5, and the cultivation density of young shellfish is 20 per layer; the mesh of the net is 1cm; the polylactic acid foam containing microelements in the mesh bag is prepared from the following raw materials in parts by mass: 100 parts of polylactic acid, 2 parts of montmorillonite and 1 part of trace element.
Wherein the microelements are composed of FeSO 4 、MgSO 4 、ZnSO 4 、Na 2 SeO 3 The mass ratio is 1:1:2:5, composing; polylactic acid is Nature works 2003D, U.S.A., melt flow rate at 210 ℃/2.16kg is 5g +.10min。
The preparation method of the polylactic acid foam containing microelements comprises the following steps:
(1) Uniformly mixing polylactic acid, montmorillonite and trace elements, and then adding the mixture into a screw extruder to be melt extruded at 160-200 ℃ to obtain polylactic acid granules containing trace elements;
(2) Placing the polylactic acid granules containing the trace elements into a high-pressure reaction kettle, and injecting supercritical CO 2 Swelling and penetrating for 30min under the conditions of 90 ℃ and 20MPa, and then rapidly releasing pressure and cooling at a pressure release speed of 20MPa/s to obtain the polylactic acid foam containing trace elements.
Example 2
A method for culturing Chlamys nobilis comprises placing young scallops in a cage, culturing in seawater, fixing mesh bag inside the cage, placing polylactic acid foam containing microelements in the mesh bag, and using 200 g/cage; the cultivation cage comprises a cultivation cage and a cover net sleeved outside the cultivation cage, wherein the diameter of the cultivation cage is 40cm, the height of the cultivation cage is 1.5m, the mesh is 2.5cm, the number of layers is 8, and the cultivation density of young shellfish is 30 per layer; the mesh of the net is 1.5cm; the polylactic acid foam containing microelements in the mesh bag is prepared from the following raw materials in parts by mass: 100 parts of polylactic acid, 4 parts of montmorillonite and 3 parts of trace elements.
Wherein the microelements are composed of FeSO 4 、MgSO 4 、ZnSO 4 、Na 2 SeO 3 The mass ratio is 2:2:3:10, the composition is as follows; polylactic acid is Nature works 2003D, U.S.A., and has a melt flow rate of 5g/10min at 210 ℃/2.16 kg.
The preparation method of the polylactic acid foam containing microelements comprises the following steps:
(1) Uniformly mixing polylactic acid, montmorillonite and trace elements, and then adding the mixture into a screw extruder to be melt extruded at 160-200 ℃ to obtain polylactic acid granules containing trace elements;
(2) Placing the polylactic acid granules containing the trace elements into a high-pressure reaction kettle, and injecting supercritical CO 2 Swelling and penetrating at 120deg.C and 20MPa for 15min, rapidly releasing pressure at 100MPa/s, and coolingHowever, a polylactic acid foam containing trace elements was obtained.
Example 3
A method for culturing Chlamys nobilis comprises placing young scallops in a cage, culturing in seawater, fixing mesh bag inside the cage, placing polylactic acid foam containing microelements in the mesh bag, and using 150 g/cage; the cultivation cage comprises a cultivation cage and a cover net sleeved outside the cultivation cage, wherein the diameter of the cultivation cage is 30cm, the height of the cultivation cage is 1.2m, the mesh is 2cm, the number of layers is 5, and the cultivation density of young shellfish is 25 per layer; the mesh of the net is 1.2cm; the polylactic acid foam containing microelements in the mesh bag is prepared from the following raw materials in parts by mass: 100 parts of polylactic acid, 3 parts of montmorillonite and 2 parts of trace elements.
Wherein the microelements are composed of FeSO 4 、MgSO 4 、ZnSO 4 、Na 2 SeO 3 The mass ratio is 1.5:1.5:2.5:7.5 parts; the method comprises the steps of carrying out a first treatment on the surface of the Polylactic acid is Nature works 2003D, U.S.A., and has a melt flow rate of 5g/10min at 210 ℃/2.16 kg.
The preparation method of the polylactic acid foam containing microelements comprises the following steps:
(1) Uniformly mixing polylactic acid, a nucleating agent and trace elements, and then adding the mixture into a screw extruder to be melt extruded at 160-200 ℃ to obtain polylactic acid granules containing the trace elements;
(2) Placing the polylactic acid granules containing the trace elements into a high-pressure reaction kettle, and injecting supercritical CO 2 Swelling and penetrating for 20min at the temperature of 100 ℃ and the pressure of 22MPa, and then rapidly decompressing and cooling at the decompression speed of 50MPa/s to obtain the polylactic acid foam containing trace elements.
Comparative example 1
A method for culturing Chlamys nobilis comprises placing young scallops in a cage, culturing in seawater, wherein the cage comprises a culturing cage and a net covering the culturing cage, the diameter of the culturing cage is 30cm, the height is 1.2m, the mesh is 2cm, the number of layers is 5, and the culturing density of the young scallops is 20 per layer; the mesh of the net is 1cm.
Comparative example 2
A method for culturing Chlamys nobilis comprises placing young scallops in a cage, culturing in seawater, fixing mesh bag inside the cage, placing polylactic acid granule containing microelements in the mesh bag, and using amount of 100 g/cage; the cultivation cage comprises a cultivation cage and a cover net sleeved outside the cultivation cage, wherein the diameter of the cultivation cage is 30cm, the height of the cultivation cage is 1.2m, the mesh is 2cm, the number of layers is 5, and the cultivation density of young shellfish is 20 per layer; the mesh of the net is 1cm; in the mesh bag, polylactic acid granules containing trace elements are prepared from the following raw materials in parts by mass: 100 parts of polylactic acid, 2 parts of montmorillonite and 1 part of trace element.
Wherein the microelements are composed of FeSO 4 、MgSO 4 、ZnSO 4 、Na 2 SeO 3 The mass ratio is 1:1:2:5, composing; polylactic acid is Nature works 2003D, U.S.A., and has a melt flow rate of 5g/10min at 210 ℃/2.16 kg.
The preparation method of the polylactic acid granules containing microelements comprises the following steps:
polylactic acid, montmorillonite and trace elements are uniformly mixed, and then added into a screw extruder to be melt extruded at 160-200 ℃ to obtain polylactic acid granules containing trace elements.
Test examples
A total of 1500 Chlamys nobilis young shellfish with a shell height of 29.1+ -1.8 mm and a mass of 3.87+ -0.86 g were selected, and the culture was carried out by the method of example 1 and comparative examples 1-2, respectively, with 500 young shellfish per group. Wherein, the sleeve cages are hung in the sea water for cultivation, and the interval between the sleeve cages is 2m. The culture start date is 4 months and 1 day, and the culture end date is 7 months and 1 day. After the cultivation is finished, the death rate and the weight growth rate are counted, and the weight growth rate is calculated according to the following formula: weight gain = (weight before cultivation-weight after cultivation)/weight before cultivation x 100%. The calculation results are shown in table 1:
TABLE 1
Project Example 1 Comparative example 1 Comparative example 2
Mortality (%) 1.6 20.2 17.8
Weight gain (%) 504.9 460.5 466.7
As can be seen from Table 1, the invention adopts the mesh bag to fix the polylactic acid foam containing trace elements in the cage during the cage culture process of the Chlamys nobilis, can improve the immunity of the scallop, obviously reduce the death rate of the scallop, and promote the growth of the scallop to a certain extent, thereby improving the yield of the Chlamys nobilis.
As a comparison, the comparative example 1 adopts a conventional cage culture method, polylactic acid foam containing trace elements is not placed, the death rate is greatly improved, the weight of the obtained finished scallop is also lower, and the yield and quality are inferior to those of the culture method of the invention; comparative example 2 the polylactic acid granules containing trace elements are fixed inside the cage by using mesh bags, and it can be seen that the mortality and weight gain rate of the polylactic acid granules are only slightly better than those of the conventional cage culture method, and the effect of the polylactic acid granules is not obviously improved, and the trace elements in the unfoamed polylactic acid granules are mainly distributed inside the polylactic acid and can be slowly released only by relying on the degradation of the polylactic acid granules, so that the content of trace elements in sea water cannot be obviously improved in the culture period, and the effect of improving the growth and immunity of the chlamys nobilis is limited.

Claims (7)

1. A method for culturing chlamys nobilis is characterized by comprising the steps of placing young scallops in a cage, culturing in seawater, wherein a net bag is fixed inside the cage, and polylactic acid foam containing microelements is placed in the net bag; the polylactic acid foam containing the trace elements is prepared from the following raw materials in parts by mass: 100 parts of polylactic acid, 2-4 parts of nucleating agent and 1-3 parts of trace elements, and the preparation method comprises the following steps: firstly, carrying out melt extrusion granulation on raw materials, and then carrying out supercritical fluid foaming to obtain polylactic acid foam containing trace elements;
the trace elements are formed by FeSO 4 、MgSO 4 、ZnSO 4 、Na 2 SeO 3 The mass ratio is (1-2): (1-2): (2-3): (5-10);
the nucleating agent is one or a mixture of a plurality of montmorillonite, talcum powder and kaolin;
the melt flow rate of the polylactic acid at 210 ℃/2.16kg is 3-8g/10min.
2. The method for culturing chlamys nobilis according to claim 1, wherein the method for preparing the polylactic acid foam containing trace elements comprises the following steps:
(1) Uniformly mixing polylactic acid, a nucleating agent and trace elements, and then adding the mixture into a screw extruder for melt extrusion to obtain polylactic acid granules containing the trace elements;
(2) Placing the polylactic acid granules containing the trace elements into a high-pressure reaction kettle, and injecting supercritical CO 2 Swelling and penetrating for a certain time under a certain temperature and pressure, then rapidly decompressing and cooling to obtain the polylactic acid foam containing microelements.
3. The method for breeding Chlamys nobilis according to claim 2, wherein in the step (1), the melt extrusion is performed at 160-200 ℃.
4. The method for culturing Chlamys nobilis according to claim 2, wherein in the step (2), swelling and infiltration are performed for 15-30min at a temperature of 90-120 ℃ and a pressure of 20-25 MPa; in the step (2), the pressure release speed of the rapid pressure release is 20-100MPa/s.
5. The method for culturing chlamys nobilis according to claim 1, wherein the cage comprises a culture cage and a net sleeved outside the culture cage, the diameter of the culture cage is 30-40cm, the height is 1.2-1.5m, the mesh is 2-2.5cm, and the number of layers is 5-8; the mesh of the sleeve net is 1-1.5cm.
6. The method for culturing chlamys nobilis according to claim 5, wherein the culturing density of young scallops in the culturing cage is 20-30 per layer.
7. The method for culturing chlamys nobilis according to claim 5, wherein the amount of the polylactic acid foam containing microelements is 100-200 g/cage.
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