CN114712446B

CN114712446B - Traditional Chinese medicine composition and preparation for treating stomach diseases and preparation method thereof

Info

Publication number: CN114712446B
Application number: CN202110009341.5A
Authority: CN
Inventors: 李剑; 王煜
Original assignee: Jinling Pharmaceutical Co ltd
Current assignee: Jinling Pharmaceutical Co ltd
Priority date: 2021-01-05
Filing date: 2021-01-05
Publication date: 2023-06-09
Anticipated expiration: 2041-01-05
Also published as: CN114712446A

Abstract

The invention discloses a traditional Chinese medicine composition for treating stomach diseases, a preparation and a preparation method thereof, wherein the traditional Chinese medicine composition comprises the following components: atractylodis rhizoma, poria, rhizoma Pinelliae Preparada, fructus Aurantii Immaturus, and cortex Magnolia officinalis; the preparation method comprises pretreating the above Chinese medicinal materials, mixing, and making into preparation. According to the disease-distinguishing theory of traditional Chinese medicine, the invention achieves the aim of treating diseases on the basis of repairing the physique of a patient and obtains outstanding clinical effects.

Description

Traditional Chinese medicine composition and preparation for treating stomach diseases and preparation method thereof

Technical Field

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition for treating stomach diseases, a preparation and a preparation method thereof.

Background

The main physiological functions of the stomach are to receive and digest food, and the stomach is to descend into the interior and exterior with the spleen. Stomach diseases often occur between 30 and 50 years old, and clinically, stomach diseases such as gastritis, gastric ulcer, gastric acid bias, duodenal ulcer and the like are the main diseases. At present, more traditional Chinese patent medicines in clinic can treat one or two diseases or distinguish parting treatment, but the traditional Chinese patent medicines have long treatment time, treat symptoms but not root cause, have large side effects, easily damage human bodies and can not achieve the effect of conditioning the physique of patients.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide a traditional Chinese medicine composition for treating stomach diseases, a preparation and a preparation method thereof. The invention has the effects of harmonizing stomach, relieving pain, invigorating stomach and promoting digestion, and achieves the aim of treating diseases on the basis of conditioning physique of patients; the traditional Chinese medicine composition is clinically used for treating diseases such as gastritis, gastric ulcer, gastric acid bias, duodenal ulcer and the like, and has quick curative effect and no toxic or side effect.

The technical scheme adopted for solving the technical problems is as follows:

a Chinese medicinal composition for treating gastropathy comprises the following components: atractylodis rhizoma, poria, rhizoma Pinelliae Preparada, fructus Aurantii Immaturus, and cortex Magnolia officinalis.

Preferably, the weight portions of the components are as follows: 50-70 parts of bighead atractylodes rhizome, 30-50 parts of poria cocos, 10-30 parts of ginger processed pinellia tuber, 20-30 parts of immature bitter orange on green skin and 5-20 parts of magnolia officinalis.

More preferably, the composition further comprises the following components: medicated leaven, malt, ligusticum chuanxiong, rhizoma cyperi, radix scutellariae, cuttlebone, betel nut and liquorice.

More preferably, the components are in parts by weight: 50-70 parts of bighead atractylodes rhizome, 30-50 parts of poria cocos, 10-30 parts of ginger processed pinellia tuber, 20-30 parts of immature bitter orange, 5-20 parts of magnolia officinalis, 20-50 parts of medicated leaven, 30-50 parts of malt, 10-20 parts of ligusticum chuanxiong hort, 10-20 parts of nutgrass galingale rhizome, 10-20 parts of radix scutellariae, 10-20 parts of cuttlebone, 10-20 parts of betel nut and 10-20 parts of liquorice.

More preferably, each dose comprises the following raw materials in parts by weight: 50 parts of bighead atractylodes rhizome, 40 parts of poria cocos, 10 parts of ginger processed pinellia tuber, 30 parts of green-coated immature bitter orange, 20 parts of magnolia officinalis, 50 parts of medicated leaven, 50 parts of malt, 10 parts of ligusticum chuanxiong hort, 10 parts of rhizoma cyperi, 10 parts of scutellaria baicalensis, 10 parts of cuttlebone, 10 parts of betel nut and 10 parts of liquorice.

More preferably, each dose comprises the following raw materials in parts by weight: 50 parts of bighead atractylodes rhizome, 30 parts of poria cocos, 30 parts of ginger processed pinellia tuber, 20 parts of green-coated immature bitter orange, 10 parts of magnolia officinalis, 20 parts of medicated leaven, 30 parts of malt, 20 parts of ligusticum chuanxiong hort, 20 parts of rhizoma cyperi, 20 parts of scutellaria baicalensis, 20 parts of cuttlebone, 20 parts of betel nut and 20 parts of liquorice.

More preferably, each dose comprises the following raw materials in parts by weight: 70 parts of bighead atractylodes rhizome, 50 parts of poria cocos, 10 parts of ginger processed pinellia tuber, 20 parts of green-coated immature bitter orange, 5 parts of magnolia officinalis, 35 parts of medicated leaven, 30 parts of malt, 15 parts of ligusticum chuanxiong hort, 15 parts of rhizoma cyperi, 15 parts of scutellaria baicalensis, 15 parts of cuttlebone, 15 parts of betel nut and 15 parts of liquorice.

A Chinese medicinal preparation for treating gastropathy comprises the Chinese medicinal composition for treating gastropathy and pharmaceutically or health food acceptable adjuvants.

A preparation method of a traditional Chinese medicine preparation for treating stomach diseases specifically comprises the following steps:

(1) Pulverizing rhizoma Atractylodis Macrocephalae, poria, rhizoma Pinelliae Preparatum, fructus Aurantii Immaturus, cortex Magnoliae officinalis, fructus Hordei Germinatus, rhizoma Ligustici Chuanxiong, rhizoma Cyperi, radix Scutellariae, endoconcha Sepiae, semen Arecae and radix Glycyrrhizae, sieving, and mixing;

(2) Decocting the medicated leaven in water, filtering to remove residues, collecting the liquid medicine, and concentrating to obtain fluid extract; (3) Mixing the powder obtained in step (1) and the fluid extract obtained in step (2) uniformly, and making into preparation.

Wherein, the processes of crushing, sieving, mixing, filtering, concentrating, preparing into a preparation and the like do not provide specific process parameters, which are all the prior art, and can be completed by a person skilled in the art through routine adjustment according to the prior art.

Preferably, the preparation method of the traditional Chinese medicine preparation for treating stomach diseases comprises the following steps:

(2) Decocting the medicated leaven in 8 times of water for 2-3 hours, filtering to remove residues, collecting liquid medicine, and concentrating to a relative density of 1-1.10g/mL to obtain fluid extract;

(3) Uniformly mixing the powder obtained in the step (1) and the fluid extract obtained in the step (2) to obtain wet medicinal powder, and preparing the Chinese medicinal preparation after boiling and drying.

Preferably, the traditional Chinese medicine preparation is a pill, a tablet, a capsule and powder.

Wherein, the preparation of pills, tablets, capsules and powder is the prior art.

The invention also protects the application of the traditional Chinese medicine preparation in preparing the medicine for treating stomach diseases.

The application method of the preparation comprises the following steps: making into various dosage forms for 3-4 times a day. The medicine is taken for 7 days as 1 course of treatment, and is taken for 5 courses of treatment continuously. During the period, the patient is not suitable to eat easily-developed food, and is prohibited from smoking and drinking, capsicum, raw garlic and the like; pregnant women, young children and children are forbidden.

The efficacy of the raw materials of each component of the invention is as follows:

white atractylodes rhizome: enter spleen and stomach meridians. Invigorating spleen, invigorating qi, eliminating dampness, promoting diuresis, relieving sweating, and preventing miscarriage. Can be used for treating spleen deficiency, anorexia, abdominal distention, diarrhea, phlegm retention, dizziness, palpitation, edema, spontaneous perspiration, and fetal movement.

Poria cocos: it enters heart, lung, spleen and kidney meridians. Induce diuresis and excrete dampness, invigorate spleen and calm heart. Can be used for treating edema, oliguria, phlegm retention, palpitation, spleen deficiency, anorexia, loose stool, diarrhea, uneasiness, palpitation, and insomnia.

Ginger processed pinellia tuber: enter spleen, stomach and lung meridians. Dry dampness and resolve phlegm, reduce adverse flow of qi and arrest vomiting, relieve distension and fullness and dissipate nodulation. Can be used for treating excessive phlegm, cough and asthma, phlegm retention, palpitation, dizziness, headache, emesis, gastric fullness, and globus hystericus; it is used externally to treat carbuncle and swelling with phlegm. Jiang ban Xia is often used for checking adverse rising energy and relieving vomiting.

Fructus Aurantii Immaturus from green coat: enter spleen and stomach meridians. Break qi, remove food retention, resolve phlegm and resolve distension and fullness. Is used for treating stagnation, distention, fullness, distending pain, diarrhea, constipation, phlegm stagnation, qi stagnation, chest obstruction, and chest stuffiness; prolapse of stomach, rectocele and uterine prolapse.

Cortex Magnoliae officinalis: enter spleen, lung, stomach and large intestine meridians. Dry dampness and remove phlegm, and lower qi and remove fullness. Is used for treating damp stagnation, gastric fullness, vomiting and diarrhea, food stagnation, qi stagnation, abdominal distention, constipation, phlegm retention, asthma and cough.

Medicated leaven: enter spleen and stomach meridians. Promote digestion and harmonize stomach. It is used for treating food stagnation.

Malt: it enters spleen, stomach and liver meridians. Promoting qi circulation, resolving food stagnation, invigorating spleen, stimulating appetite, promoting lactation and relieving distention. Can be used for treating dyspepsia, abdominal distention and pain, spleen deficiency, anorexia, galactostasis, breast pain, and female weaning.

Ligusticum wallichii: enter the liver and gallbladder meridians. Promoting qi circulation, resolving stagnation, dispelling pathogenic wind, removing dampness, promoting blood circulation, and relieving pain. For headache, dizziness, hypochondriac pain, abdominal pain, cold arthralgia, spasm of tendons, amenorrhea, dystocia, puerperal stasis, block pain, carbuncle, cellulitis and pyocutaneous disease. Can be used for treating menoxenia, amenorrhea, dysmenorrhea, abdominal pain, chest and hypochondrium pain, traumatic injury, headache, and rheumatalgia.

And (3) rhizoma cyperi: it enters liver, spleen and triple energizer meridians. Promoting qi circulation, resolving stagnation, regulating menstruation and relieving pain. Can be used for treating liver depression, qi stagnation, chest pain, hypochondrium pain, abdominal distention and pain, dyspepsia, chest distress, cold hernia abdominal pain, breast pain, menoxenia, amenorrhea and dysmenorrhea.

Radix Scutellariae Baicalensis: it enters lung, gallbladder, spleen, large intestine and small intestine meridians. Clear heat and dry dampness, purge fire and detoxify, stop bleeding and prevent abortion. Can be used for treating damp-heat, summer-heat, chest distress, emesis, and malignant boil, damp-heat distention and fullness, dysentery, jaundice, cough due to lung heat, polydipsia due to high fever, hematemesis, carbuncle, skin sore, and fetal movement.

Cuttlebone: it enters liver and kidney meridians. Astringing to stop bleeding, astringing essence to stop leukorrhagia, producing acid, and healing sore. Can be used for treating gastralgia, acid regurgitation, hematemesis, epistaxis, metrorrhagia, hematochezia, spermatorrhea, and leucorrhea with reddish discharge; ulcer disease. It is indicated for traumatic hemorrhage, sore and purulent.

Betel nut: enter stomach and large intestine meridians. Expelling parasites, removing food retention, lowering qi and promoting the circulation of water. Can be used for treating taeniasis, ascariasis, schistosomiasis, abdominal pain due to accumulation of worms, diarrhea due to accumulation of food stagnation, tenesmus, edema, tinea pedis, and malaria.

Licorice root: it enters heart, lung, spleen and stomach meridians. Spleen invigorating, qi replenishing, heat and toxic materials clearing away, phlegm eliminating, cough relieving, pain relieving, and medicines regulating. Can be used for treating weakness of spleen and stomach, listlessness, debilitation, palpitation, short breath, cough with excessive phlegm, abdominal pain, limb spasm, carbuncle, swelling, sore and toxic materials, and relieving drug toxicity and intensity.

Specifically, in the invention, bighead atractylodes rhizome, poria cocos and pinellia tuber are used as monarch drugs for strengthening spleen and eliminating dampness; fructus Aurantii Immaturus, cortex Magnolia officinalis, and cortex Magnolia officinalis are ministerial drugs for warming middle warmer and regulating qi; medicated leaven, malt, szechuan lovage rhizome, nutgrass galingale rhizome, baical skullcap root, cuttlebone and betel nut have the effects of resolving food stagnation, dampness, qi, blood, phlegm, fire, insects and the like and are used as adjuvants; licorice root, radix Glycyrrhizae regulates the flow of the other drugs and also supplements the middle warmer.

Advantageous effects

Compared with the prior art, the invention treats according to the disease differentiation theory of traditional Chinese medicine, uses medicated leaven, malt, szechuan lovage rhizome, nutgrass galingale rhizome, baical skullcap root, cuttlebone and betel nut cooperatively, has the effects of resolving food, dampness, qi, blood, phlegm, fire, insects and the like, achieves the aim of treating diseases on the basis of repairing physique of patients, and achieves outstanding clinical effects. The traditional Chinese medicine composition is clinically used for treating diseases such as gastritis, gastric ulcer, gastric acid bias, duodenal ulcer and the like, and has quick curative effect and no toxic or side effect.

Drawings

FIG. 1 is a diagram of a stomach tissue anatomy; wherein A is blank group; b is a building module; c is Sanjiuweitai group (positive drug); d is the low dose group of the present application; e is the dosage group herein; f is the high dose group of the present application;

FIG. 2 shows the ulcer score (A) and the ulcer area (B)

n＝5，####P<0.0001,*P<0.05,**P<0.01)；

FIG. 3 is a graph of gastric wall longitudinal HE pathology (scale bar 100 μm); wherein A is a blank group; b is a building module; c is Sanjiuweitai group (positive drug); d is the low dose group of the present application; e is the dosage group herein; f is the high dose group of the present application;

FIG. 4 shows the MPO content (A) and SOD content (B) of stomach tissue; (

n＝5，####P<0.0001,*P<0.05,***P<0.001,****P<0.0001)。

Detailed Description

The present invention will be described in further detail with reference to examples. The reagents or instrumentation used are not manufacturer specific and are considered to be commercially available conventional products.

Example 1

A Chinese medicinal composition for treating gastropathy comprises the following raw materials in part by weight: 50 parts of bighead atractylodes rhizome, 50 parts of poria cocos, 10 parts of ginger processed pinellia tuber, 20 parts of green-coat immature bitter orange and 10 parts of magnolia officinalis.

The preparation method of the traditional Chinese medicine composition for treating stomach diseases comprises the following steps:

(2) And (3) boiling and drying the powder obtained in the step (1) and then preparing into pills, tablets, capsules or powder.

Example 2

A Chinese medicinal composition for treating gastropathy comprises the following raw materials in part by weight: 70 parts of bighead atractylodes rhizome, 40 parts of poria cocos, 20 parts of ginger processed pinellia tuber, 30 parts of immature bitter orange on green skin and 20 parts of magnolia officinalis.

The preparation method of the traditional Chinese medicine composition for treating stomach diseases is the same as that of example 1.

Example 3

A Chinese medicinal composition for treating gastropathy comprises the following raw materials in part by weight: 60 parts of bighead atractylodes rhizome, 30 parts of poria cocos, 30 parts of ginger processed pinellia tuber, 25 parts of green-coat immature bitter orange and 5 parts of magnolia officinalis.

Example 4

A Chinese medicinal composition for treating gastropathy comprises the following raw materials in part by weight: 50 parts of bighead atractylodes rhizome, 40 parts of poria cocos, 10 parts of ginger processed pinellia tuber, 30 parts of green-coated immature bitter orange, 20 parts of magnolia officinalis, 50 parts of medicated leaven, 50 parts of malt, 10 parts of ligusticum chuanxiong hort, 10 parts of rhizoma cyperi, 10 parts of scutellaria baicalensis, 10 parts of cuttlebone, 10 parts of betel nut and 10 parts of liquorice.

Example 5

A Chinese medicinal composition for treating gastropathy comprises the following raw materials in part by weight: 50 parts of bighead atractylodes rhizome, 30 parts of poria cocos, 30 parts of ginger processed pinellia tuber, 20 parts of green-coated immature bitter orange, 10 parts of magnolia officinalis, 20 parts of medicated leaven, 30 parts of malt, 20 parts of ligusticum chuanxiong hort, 20 parts of rhizoma cyperi, 20 parts of scutellaria baicalensis, 20 parts of cuttlebone, 20 parts of betel nut and 20 parts of liquorice.

Example 6

A Chinese medicinal composition for treating gastropathy comprises the following raw materials in part by weight: 70 parts of bighead atractylodes rhizome, 50 parts of poria cocos, 10 parts of ginger processed pinellia tuber, 20 parts of green-coated immature bitter orange, 5 parts of magnolia officinalis, 35 parts of medicated leaven, 30 parts of malt, 15 parts of ligusticum chuanxiong hort, 15 parts of rhizoma cyperi, 15 parts of scutellaria baicalensis, 15 parts of cuttlebone, 15 parts of betel nut and 15 parts of liquorice.

(3) Uniformly mixing the powder obtained in the step (1) and the fluid extract obtained in the step (2) to obtain wet medicinal powder, and boiling and drying to obtain the traditional Chinese medicine preparation.

Example 7

(3) Mixing the powder obtained in step (1) and the fluid extract obtained in step (2) uniformly, and making into pill, tablet, capsule or powder.

Example 8 acute toxicity test

(1) Medicament: according to the traditional Chinese medicine preparation, the traditional Chinese medicine is dissolved.

(2) Test animals: mice, weighing about 50g, were randomly selected from male and female animals.

(3) Method and results: the method is characterized in that 50 mice are taken and divided into 5 groups, each group is divided into 10 mice, hungry is performed for about 15 hours, 20mL of each group is filled with medicine, 1, 2, 3, 4 and 5 groups are respectively filled with medicine 1, 2, 3, 4 and 5 times within 24 hours, and the mice are observed for 5 days and normally move, have no toxic reaction and are free from death. Wherein, the dosage of the mice is calculated according to the dosage of kilogram body weight, which shows that the preparation has no acute toxicity and safe clinical dosage.

Example 9

In order to explore whether the traditional Chinese medicine preparation has a protective effect on acute gastric ulcer or not, absolute ethyl alcohol is adopted to establish a mouse acute gastric ulcer model, and the protective effect on acute gastric ulcer is evaluated by measuring indexes such as the area and index of the mouse gastric ulcer, the content of Myeloperoxidase (MPO) and superoxide dismutase (SOD) in stomach tissues and the like.

1. Material

1.1 Medicament

The positive medicine used in the experiment is Sanjiuweitai, and the tested medicine is the traditional Chinese medicine preparation in the example 5 of the application.

1.2 reagents

Sodium chloride injection, TMB color development liquid, superoxide dismutase (SOD) kit and 4% paraformaldehyde.

1.3 animals

ICR mice, SPF grade males, body weight (28.0.+ -. 1.0) g, 6 weeks of age.

2 method

2.1 animal acute gastric ulcer model establishment

1. The mice were dosed for 2 weeks with continuous gavage, and the blank and model were perfused with equivalent doses of saline. The mice in each group are fasted for 24 hours before the last administration, water is not forbidden, and the mice in the administration group and the model group are infused with absolute ethyl alcohol according to the ratio of 10ml/kg to cause acute gastric ulcer model (Shi Lina, wang Xuefei, etc. the effective part of the pericarpium Granati polyphenol is toxic after single administration and has protection effect on the gastric ulcer of the rats caused by the absolute ethyl alcohol [ J ]. Chinese pharmacology report, 2015,31 (5): 709-15); the mice in the blank group are lavaged with 10ml/kg physiological saline; all mice were sacrificed after 4h of fasting and water deprivation.

2.2 grouping and administration

Animals are received and placed in animal houses for a week to be fed with the animals for adapting, and then the animals are randomly divided into 6 groups, 5 animals in each group, namely a blank group, a model group, a positive medicine group (Sanjiuweitai), a low-dose group in the application, a medium-dose group in the application and a high-dose group in the application. Each administration group was administered by intragastric administration at a dose of 10ml/kg, and the administration concentrations were respectively, SANJIUWEITAI (2000 mg/kg), the low dose (62.5 mg/kg) of the present application, the medium dose (125 mg/kg) of the present application, and the high dose (250 mg/kg) of the present application; the blank and model groups were given saline by gavage at a dose of 10 ml/kg. Oral administration was carried out 1 time per day for 2 weeks.

2.3 detection of gastric ulcer disease index

After the animals are euthanized, the stomach tissues are taken out, a small opening is cut at the cardiac position, then the stomach is cut along the large curve of the stomach, the contents are gently washed clean by normal saline, then the contents are spread in a clean culture dish for photographing, and the number of blood spots is calculated. The extent of injury is expressed in terms of ulcer score, ulcer area, and ulcer inhibition, respectively. The ulcer scoring criteria were as follows: normal stomach color, 0; red, 0.5; punctiform ulcers, 1.0; streak bleeding, 1.5; deep ulcers, 2.0; gastric perforation, 3.0; the ulcer index was calculated as follows: ui= (un+us+up) ×10 ^－1 Where UN refers to the average number of ulcers per mouse, US refers to the average number of severe ulcer scores, UP refers to the percentage of gastric ulcerated mice ^[3] The method comprises the steps of carrying out a first treatment on the surface of the The gastric ulcer area was calculated from the photographs taken using software Image J.

2.4 detection of biochemical indicators of gastric tissue and pathological examination

2.4.1 detection of MPO Activity and SOD Activity of stomach tissue

The stomach tissue of the same part of each mouse was taken out to 40mg in an EP tube, and pre-chilled physiological saline, i.e., 400. Mu.l, was added at a concentration of 10ml/g, and the tissue was cut with scissors in ice and then ground with an electric tissue grinder to obtain a tissue homogenate. The resulting homogenate was centrifuged at 12000r/min for 10min (4 ℃) and the supernatant was collected and assayed for MPO and SOD enzyme activity according to the kit instructions.

2.5.2 histopathological evaluation

Then taking the stomach tissues of the same part of each mouse, spreading the stomach tissues on tinfoil paper, fixing the stomach tissues for 24 hours by using 4% paraformaldehyde, and carrying out embedding, slicing and hematoxylin-eosin (HE) staining. Histopathological evaluation was performed after observation photographing using an inverted microscope.

2.6 statistical methods

Statistical analysis of the data was performed using GraphPad Prism 8.0 software. The blank control group and the model building group adopt t test, and the model building group and the administration group adopt one-way ANOVA test.

3 results

3.1 effects of drug administration and modeling on mouse status

The status of mice was examined by their body weight and behavioral manifestations. After 14 days of continuous administration, the weight of mice in the administration group and the normal saline administration group is not obviously reduced, and the mice have no symptoms of hair shrugging, unhairing, listlessness and the like. The application and the Sanjiuweitai are both free from obvious drug toxicity.

The mice with the ethanol modeling modules have unstable walking, sleepiness, listlessness, cold feeling and the like, and the positive drug group, the dosage in the application and the high-dosage mice in the application are more awake and more active than the mice with the model groups. The low dose group and the modeling group of the application have no obvious difference in behavioral performance.

3.2 detection results of gastric ulcer related index

3.2.1 stomach tissue anatomical examination of mice

Macroscopic after gastric tissue dissection (fig. 1): the stomach tissue of the blank control group is in meat powder color, and the stomach wall is intact without bleeding points; compared with the blank group, the stomach wall of the ethanol making module is red, and large-area ulcers and bleeding appear; compared with a model group, the bleeding points of the positive medicine group are obviously reduced, and the large area is meat pink; the low-dose group stomach tissue is red, and bleeding and ulcers appear in a larger area; the tissue of the dosage group is slightly red, and bleeding and ulcers appear in a small area; the high dose tissue of the application is in a meat pink color, and sporadic bleeding points appear. At the same time, the ulcer index results suggest that the high dose group of the present application has a lower ulcer index and statistically significant differences compared to the modeling group (fig. 2), and that the positive drug group and the low and medium dose groups of the present application have lower ulcer index but statistically no significant differences compared to the modeling group. In addition, the area of ulcers for the Sanjiuweitai group, the dose in this application, and the high dose group in this application were all lower than the modeling group and were statistically significantly different, as were the low dose groups in this application (fig. 2).

3.2.2 stomach histopathological changes in mice

As shown in fig. 3, the glands of stomach tissues of the mice in the blank group are orderly arranged, the mucous membrane is in a complete structure, the submucosa and the myometrium are in a clear layer, and edema and inflammatory cell infiltration are not seen. The normal tissue structure of the modeling module disappears, the cells of the top gland are largely shed, the mucous membrane layer is seriously damaged, and obvious inflammatory cell infiltration exists. Submucosa is widened, i.e. oedema. Compared with a modeling module, the submucosa of the positive medicine group is widened partially, and the top gland cells are less in shedding; the low-dose group has more apical gland cells shed and a large amount of inflammatory cells infiltrate, and does not show a protective effect; in the application, a small amount of apical gland cells infiltrate in a dosage group, and partial inflammatory cells infiltrate, so that the protection effect is not obvious; the high-dose group stomach tissue structure is completed, the edema phenomenon is obviously improved, and the glandular cell shedding phenomenon does not exist at the top end, so that the high-dose group has a certain protection effect on gastric ulcer.

3.3 Effect on the Biochemical index of gastric tissue in acute gastric ulcer mice

As shown in fig. 4, compared with the blank control group, the activity of the MPO-based peroxidase in the stomach tissue of the model group was significantly increased, and the SOD content was significantly decreased, suggesting that ethanol caused oxidative damage to the body. Compared with the modeling module, the peroxidase activity of the high-dose group is obviously reduced, the SOD content is increased, and the medicine of the application prompts that the organism can metabolically generate more SOD to resist the increased peroxidase, so that the medicine has certain antioxidant capacity.

Conclusion 4

The results show that the method can obviously reduce the ulcer score and the ulcer area of the ethanol gastric ulcer of the mice, and has a certain ulcer inhibition rate. Biochemical index analysis suggests that administration of the composition can remarkably reduce peroxidase level in stomach tissues and increase SOD content, and that the composition has a protective effect on gastric ulcers caused by ethanol, and mainly exerts the protective effect on mucosal injury by reducing inflammation and resisting oxidation, and the effect has dose dependency. A dose of the present application may be used to prevent an alcoholic gastric ulcer.

Example 10 typical case

Case 1

The symptoms of men who are 38 years old and who are fond of pungent foods such as sour and hot foods, and have hidden pain in the stomach for the last several months, are diagnosed as gastric ulcers after examination, are basically eliminated after 4 treatment courses of taking the medicament of the embodiment 5 of the invention, and the clinical symptoms are completely eliminated after the 2 treatment courses of continuing to take the medicament of the embodiment 5 of the invention.

Case 2

Women who are 33 years old, have abdominal pain for one year, are aggravated on an empty stomach, have some relief after meals, and have sometimes returned acid. After taking 6 medicaments of the invention for 2 courses of treatment, each symptom is obviously relieved, and then taking the medicament of the invention for 1 course of treatment, clinical symptoms are eliminated.

The protection of the present invention is not limited to the above embodiments. Variations and advantages that would occur to one skilled in the art are included in the invention without departing from the spirit and scope of the inventive concept, and the scope of the invention is defined by the appended claims.

Claims

1. The traditional Chinese medicine composition for treating gastric ulcer is characterized by being prepared from the following raw materials in parts by weight: 50-70 parts of bighead atractylodes rhizome, 30-50 parts of poria cocos, 10-30 parts of ginger processed pinellia tuber, 20-30 parts of immature bitter orange, 5-20 parts of magnolia officinalis, 20-50 parts of medicated leaven, 30-50 parts of malt, 10-20 parts of ligusticum wallichii, 10-20 parts of nutgrass galingale rhizome, 10-20 parts of radix scutellariae, 10-20 parts of cuttlebone, 10-20 parts of betel nut and 10-20 parts of liquorice.

2. The traditional Chinese medicine composition for treating gastric ulcer according to claim 1, wherein the composition is prepared from the following raw materials in parts by weight: 50 parts of bighead atractylodes rhizome, 40 parts of poria cocos, 10 parts of ginger processed pinellia tuber, 30 parts of green-coated immature bitter orange, 20 parts of magnolia officinalis, 50 parts of medicated leaven, 50 parts of malt, 10 parts of ligusticum wallichii, 10 parts of rhizoma cyperi, 10 parts of scutellaria baicalensis, 10 parts of cuttlebone, 10 parts of betel nut and 10 parts of liquorice.

3. The traditional Chinese medicine composition for treating gastric ulcer according to claim 1, wherein the composition is prepared from the following raw materials in parts by weight: 50 parts of bighead atractylodes rhizome, 30 parts of poria cocos, 30 parts of ginger processed pinellia tuber, 20 parts of green-coated immature bitter orange, 10 parts of magnolia officinalis, 20 parts of medicated leaven, 30 parts of malt, 20 parts of ligusticum wallichii, 20 parts of rhizoma cyperi, 20 parts of scutellaria baicalensis, 20 parts of cuttlebone, 20 parts of betel nut and 20 parts of liquorice.

4. The traditional Chinese medicine composition for treating gastric ulcer according to claim 1, wherein the composition is prepared from the following raw materials in parts by weight: 70 parts of bighead atractylodes rhizome, 50 parts of poria cocos, 10 parts of ginger processed pinellia tuber, 20 parts of green-coated immature bitter orange, 5 parts of magnolia officinalis, 35 parts of medicated leaven, 30 parts of malt, 15 parts of ligusticum wallichii, 15 parts of rhizoma cyperi, 15 parts of scutellaria baicalensis, 15 parts of cuttlebone, 15 parts of betel nut and 15 parts of liquorice.

5. A traditional Chinese medicine preparation for treating gastric ulcer, which is characterized by comprising the traditional Chinese medicine composition for treating gastric ulcer according to any one of claims 1-4 and pharmaceutically acceptable auxiliary materials.

6. The preparation method of the traditional Chinese medicine preparation for treating gastric ulcer as claimed in claim 5, which is characterized by comprising the following specific steps:

(1) Pulverizing rhizoma Atractylodis Macrocephalae, poria, rhizoma Pinelliae Preparatum, fructus Aurantii Immaturus, cortex Magnoliae officinalis, fructus Hordei Germinatus, rhizoma Chuanxiong, rhizoma Cyperi, radix Scutellariae, endoconcha Sepiae, semen Arecae, and radix Glycyrrhizae, sieving, and mixing;

(2) Decocting the medicated leaven in water, filtering to remove residues, collecting the liquid medicine, and concentrating to obtain fluid extract;

(3) Mixing the powder obtained in step (1) and the fluid extract obtained in step (2) uniformly, and making into preparation.

7. The method for preparing a Chinese medicinal preparation for treating gastric ulcer according to claim 6, wherein the preparation is a pill, a tablet, a capsule or a powder.

8. The method for preparing the traditional Chinese medicine preparation for treating gastric ulcer according to claim 6, which is characterized by comprising the following specific steps: