CN114712261A - Nano-liposome containing ceramide-like extract and preparation method and application thereof - Google Patents
Nano-liposome containing ceramide-like extract and preparation method and application thereof Download PDFInfo
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- CN114712261A CN114712261A CN202210339058.3A CN202210339058A CN114712261A CN 114712261 A CN114712261 A CN 114712261A CN 202210339058 A CN202210339058 A CN 202210339058A CN 114712261 A CN114712261 A CN 114712261A
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- ceramide
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- phospholipid
- mixed solution
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- 239000000284 extract Substances 0.000 title claims abstract description 57
- 239000002502 liposome Substances 0.000 title claims abstract description 46
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 238000000605 extraction Methods 0.000 title description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 39
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 32
- 239000002245 particle Substances 0.000 claims abstract description 25
- 235000012000 cholesterol Nutrition 0.000 claims abstract description 16
- 150000003904 phospholipids Chemical class 0.000 claims abstract description 16
- 150000005846 sugar alcohols Polymers 0.000 claims abstract description 10
- 229940106189 ceramide Drugs 0.000 claims description 31
- 239000011259 mixed solution Substances 0.000 claims description 30
- YDNKGFDKKRUKPY-JHOUSYSJSA-N C16 ceramide Natural products CCCCCCCCCCCCCCCC(=O)N[C@@H](CO)[C@H](O)C=CCCCCCCCCCCCCC YDNKGFDKKRUKPY-JHOUSYSJSA-N 0.000 claims description 29
- CRJGESKKUOMBCT-VQTJNVASSA-N N-acetylsphinganine Chemical compound CCCCCCCCCCCCCCC[C@@H](O)[C@H](CO)NC(C)=O CRJGESKKUOMBCT-VQTJNVASSA-N 0.000 claims description 29
- ZVEQCJWYRWKARO-UHFFFAOYSA-N ceramide Natural products CCCCCCCCCCCCCCC(O)C(=O)NC(CO)C(O)C=CCCC=C(C)CCCCCCCCC ZVEQCJWYRWKARO-UHFFFAOYSA-N 0.000 claims description 29
- VVGIYYKRAMHVLU-UHFFFAOYSA-N newbouldiamide Natural products CCCCCCCCCCCCCCCCCCCC(O)C(O)C(O)C(CO)NC(=O)CCCCCCCCCCCCCCCCC VVGIYYKRAMHVLU-UHFFFAOYSA-N 0.000 claims description 29
- 238000003756 stirring Methods 0.000 claims description 21
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims description 12
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 claims description 11
- UWJJYHHHVWZFEP-UHFFFAOYSA-N pentane-1,1-diol Chemical compound CCCCC(O)O UWJJYHHHVWZFEP-UHFFFAOYSA-N 0.000 claims description 11
- 230000000694 effects Effects 0.000 claims description 8
- 238000000265 homogenisation Methods 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 7
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 229940067606 lecithin Drugs 0.000 claims description 4
- 239000000787 lecithin Substances 0.000 claims description 4
- 235000010445 lecithin Nutrition 0.000 claims description 4
- 230000003020 moisturizing effect Effects 0.000 claims description 3
- 239000008347 soybean phospholipid Substances 0.000 claims description 3
- JQWAHKMIYCERGA-UHFFFAOYSA-N (2-nonanoyloxy-3-octadeca-9,12-dienoyloxypropoxy)-[2-(trimethylazaniumyl)ethyl]phosphinate Chemical compound CCCCCCCCC(=O)OC(COP([O-])(=O)CC[N+](C)(C)C)COC(=O)CCCCCCCC=CCC=CCCCCC JQWAHKMIYCERGA-UHFFFAOYSA-N 0.000 claims description 2
- CFWRDBDJAOHXSH-SECBINFHSA-N 2-azaniumylethyl [(2r)-2,3-diacetyloxypropyl] phosphate Chemical compound CC(=O)OC[C@@H](OC(C)=O)COP(O)(=O)OCCN CFWRDBDJAOHXSH-SECBINFHSA-N 0.000 claims description 2
- 230000002087 whitening effect Effects 0.000 claims description 2
- 239000002537 cosmetic Substances 0.000 abstract description 10
- 239000002994 raw material Substances 0.000 abstract 1
- 239000000203 mixture Substances 0.000 description 7
- 239000008213 purified water Substances 0.000 description 7
- 210000003491 skin Anatomy 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 239000013543 active substance Substances 0.000 description 4
- 150000001408 amides Chemical class 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 238000010008 shearing Methods 0.000 description 3
- WWUZIQQURGPMPG-UHFFFAOYSA-N (-)-D-erythro-Sphingosine Natural products CCCCCCCCCCCCCC=CC(O)C(N)CO WWUZIQQURGPMPG-UHFFFAOYSA-N 0.000 description 2
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 2
- JCYZMTMYPZHVBF-UHFFFAOYSA-N Melarsoprol Chemical compound NC1=NC(N)=NC(NC=2C=CC(=CC=2)[As]2SC(CO)CS2)=N1 JCYZMTMYPZHVBF-UHFFFAOYSA-N 0.000 description 2
- 125000000217 alkyl group Chemical group 0.000 description 2
- -1 amide compounds Chemical class 0.000 description 2
- 230000000975 bioactive effect Effects 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 150000001783 ceramides Chemical class 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- WWUZIQQURGPMPG-KRWOKUGFSA-N sphingosine Chemical compound CCCCCCCCCCCCC\C=C\[C@@H](O)[C@@H](N)CO WWUZIQQURGPMPG-KRWOKUGFSA-N 0.000 description 2
- 210000000434 stratum corneum Anatomy 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 229930186217 Glycolipid Natural products 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- YDNKGFDKKRUKPY-TURZORIXSA-N N-hexadecanoylsphingosine Chemical compound CCCCCCCCCCCCCCCC(=O)N[C@@H](CO)[C@H](O)\C=C\CCCCCCCCCCCCC YDNKGFDKKRUKPY-TURZORIXSA-N 0.000 description 1
- 239000003876 biosurfactant Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 125000001549 ceramide group Chemical group 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 210000001723 extracellular space Anatomy 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000004973 liquid crystal related substance Substances 0.000 description 1
- 150000004668 long chain fatty acids Chemical class 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000008591 skin barrier function Effects 0.000 description 1
- 229940083466 soybean lecithin Drugs 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 239000008215 water for injection Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/68—Sphingolipids, e.g. ceramides, cerebrosides, gangliosides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/14—Liposomes; Vesicles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/55—Phosphorus compounds
- A61K8/553—Phospholipids, e.g. lecithin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/20—Chemical, physico-chemical or functional or structural properties of the composition as a whole
- A61K2800/26—Optical properties
- A61K2800/262—Transparent; Translucent
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/41—Particular ingredients further characterized by their size
- A61K2800/413—Nanosized, i.e. having sizes below 100 nm
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/56—Compounds, absorbed onto or entrapped into a solid carrier, e.g. encapsulated perfumes, inclusion compounds, sustained release forms
Abstract
The invention discloses a nanoliposome containing a ceramide-like extract, and a preparation method and application thereof. The invention prepares the nano-liposome containing the ceramide-like extract by taking the ceramide-like extract, cholesterol, phospholipid, glycerol, polyalcohol and water as raw materials. The nano liposome is semitransparent or transparent, and the average particle size is less than 200 nm; the ceramide-like nano liposome has high stability and good water dispersibility, can be directly used as cosmetics and can be optionally matched with a cosmetic formula, so that the difficult problem that the ceramide-like nano liposome is difficult to directly apply to the cosmetics is solved; the preparation method is simple and controllable, and has good repeatability.
Description
Technical Field
The invention relates to the technical field of carrier systems in the cosmetic preparation technology, in particular to a nanoliposome containing a ceramide-like extract and a preparation method and application thereof.
Background
Ceramide (Ceramide), namely Ceramide N-acyl Sphingosine, is a class of amide compounds formed by condensing Sphingosine (Sphingosine) and long-chain fatty acid, is a class of compounds rather than a compound, and mainly exists in cell membranes and intercellular matrixes of stratum corneum. The structure of the amide is characterized in that the amide also contains two lipophilic long-chain alkyl groups and two hydrophilic hydroxyl groups besides the amide structure. The invention relates to a ceramide-like extract which is a ceramide-like (mannose erythritol esters, Mels) extract applied in the earlier stage of the company (Chinese patent application No. 201910667983.7), is a glycolipid biosurfactant component, and is found to contain a spatial structure similar to ceramide, and two long-chain alkyl groups similar to ceramide and two or more hydrophilic hydroxyl groups. In addition, Mels easily penetrates into the intercellular spaces of the stratum corneum of the skin, forms liquid crystals, maintains skin moisture, has moisturizing, skin moistening and skin barrier repair effects, and has partial effects similar to those of ceramide. Most of the existing ceramides are obtained by separating animals and plants, and the existing ceramides have the disadvantages of low yield, extremely high economic cost and great limitation on application. Therefore, the ceramide in the invention has similar ceramide structure and effect, is safe and nontoxic, can be used as a substitute of ceramide, improves the effect and reduces the cost.
The Liposome (Liposome) has very close structure with the plasma membrane system of the human body, namely the most basic structure and functional unit, namely 'cell', and has very good physiological compatibility with the human body, so that when the Liposome is used as a carrier system, the human body has little rejection reaction to the Liposome; the membrane structure of the liposome mainly comprises phospholipid and cholesterol, the phospholipid is used as the basis of the membrane structure of the liposome, the cholesterol plays a role in stabilizing the liposome structure, and the cholesterol can play a role in enhancing the stability of the liposome structure when environmental conditions are changed (such as temperature, osmotic pressure, pH and the like); the ceramide is a novel bioactive substance, mainly contains three or more Mels active substance components of Mel-A (mannose-1-erythritol-2, 3-dicamba-4, 6-diacetate), Mel-B (mannose-1-erythritol-2, 3-dicamba-6-acetate) and Mel-C (mannose-1-erythritol-2, 3-dicamba-4-acetate), and the content of the three Mels active substance components in the ceramide-like extract is more than 45%; ceramide-like compounds have wide development prospects in skin and hair care applications, and cosmetics containing ceramide-like compounds form a research hotspot. There have been many studies reporting that the prevention of water loss in the skin and the inhibition of melanin brown spots can be achieved by using a product such as an external cream containing a ceramide. However, the currently prepared ceramide-like extract is oily liquid, the stability and the water dispersibility of the ceramide-like extract are poor, and the ceramide-like extract is difficult to be directly applied to cosmetics and the like.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide the nanoliposome containing the ceramide extract, which has high stability and good water dispersibility and can be directly applied to cosmetics.
The invention also aims to provide a preparation method of the nanoliposome containing the ceramide-like extract.
The invention also aims to provide the application of the nano-liposome containing the ceramide-like extract.
In order to achieve the purpose, the invention adopts the following technical scheme:
a nanoliposome containing a ceramide-like extract comprises the following components in percentage by mass: 1-10% of ceramide-like extract, 0.0-3.0% of cholesterol, 3-15% of phospholipid, 25-50% of glycerol, 5-25% of polyalcohol and the balance of water; preferably comprises the following components in percentage by mass: 3-8% of ceramide extract, 0.2-0.5% of cholesterol, 10% of phospholipid, 30-35% of glycerol, 17-18% of polyalcohol and the balance of water.
The ceramide-like extract is an extract containing a novel Mels bioactive substance prepared and extracted by a bio-emulsion fermentation method (refer to patent application CN201910667983.7), mainly contains three or more Mels active substance components of Mel-A, Mel-B, Mel-C, and has the effect similar to ceramide, wherein the content of the three Mels active substance components in the ceramide-like extract is more than 45%.
The phospholipid is at least one of hydrogenated phospholipid, liquid lecithin, soybean phospholipid, egg yolk lecithin and cephalin.
The hydrogenated phospholipid is preferably at least one of hydrogenated lecithin and hydrogenated soybean phospholipid.
The purity of the phospholipids is more than or equal to 70 percent.
The polyalcohol is at least one of butanediol and pentanediol; preferably a combination of butanediol and pentanediol; more preferably butanediol and pentanediol in a mass ratio of 15: 2 to obtain the compound.
The water is preferably at least one of deionized water, purified water and water for injection.
The particle size of the nano liposome containing the ceramide-like extract is 50-700nm, the average particle size is less than 200nm, and the nano liposome is uniformly distributed and is semitransparent or transparent; the preferable particle size is 60-650nm, and the average particle size is 179-197 nm.
The preparation method of the nanoliposome containing the ceramide-like extract comprises the following steps:
(1) heating ceramide-like extract to 70-80 deg.C, adding phospholipid, cholesterol and polyalcohol, and stirring at 70-80 deg.C to obtain uniform mixed solution A;
(2) adding glycerol into the mixed solution A at the temperature of 70-80 ℃, and uniformly stirring to obtain a uniform mixed solution B;
(3) adding water into the mixed solution B at the temperature of 70-80 ℃, and uniformly stirring to obtain a uniform mixed solution C;
(4) and (3) keeping the temperature of the mixed solution C at 70-75 ℃, and homogenizing to obtain the nanoliposome containing the ceramide-like extract.
The heating degree in the step (1) is preferably 75-80 ℃.
The stirring in the step (1) is shear stirring, and the condition of the shear stirring is preferably 120-240 rpm in order to obtain a uniform system with uniform mixing.
The temperature in the step (2) is preferably 70-75 ℃.
The temperature in the step (3) is preferably 70-75 ℃.
The homogenization conditions in step (4) are preferably as follows: homogenizing for 3-10 times at high pressure of 35-40MPa, and homogenizing for the last time at 80 MPa; more preferably: homogenizing for 5-6 times under high pressure of 35-40MPa, and homogenizing for the last time under 80 MPa.
The application of the nano-liposome containing the ceramide-like extract in preparing daily chemical products.
The daily chemical product has at least one of the following effects: moisturizing, caring skin, whitening, and removing speckle.
Compared with the prior art, the invention has the following advantages and effects:
1. according to the invention, the oily ceramide-like extract is prepared into the nano liposome, so that the problems that the ceramide-like extract is poor in water dispersibility and difficult to directly apply to cosmetics are effectively solved, and meanwhile, the ceramide-like extract is substituted for ceramide, so that the application resources of ceramide are expanded;
2. because the liposome membrane structure can play a role in enhancing the stability of the liposome structure, the ceramide-like extract is wrapped in the nano-liposome to be free from or less influenced by the change of environmental conditions, and the stability of the ceramide-like extract is improved.
3. The preparation method of the ceramide-like extract liposome has the advantages of simple process, high stability of the nano liposome, good water dispersibility and good biocompatibility, and can be directly used as cosmetics and optionally matched with a cosmetic formula; the preparation method is controllable and has good repeatability.
Drawings
Fig. 1 is a distribution diagram of the particle size of the nanoliposome containing the ceramide-like extract obtained in example 1.
Fig. 2 is a distribution diagram of the particle size of the nanoliposome containing the ceramide-like extract obtained in example 2.
Fig. 3 is a distribution diagram of the particle size of the nanoliposome containing the ceramide-like extract obtained in example 3.
Detailed Description
The above embodiments are further described with reference to the following detailed description and accompanying drawings. The following description is only for the purpose of illustrating the objects, effects and technical features of the present invention, and does not limit the scope of the present invention.
EXAMPLE 1 preparation of nanoliposomes containing ceramide-like extracts
1. The composition of the liposome system is shown in table 1:
TABLE 1 nanoliposome system components containing ceramide-like extract composition one
| Prescription composition | Quality (g) | Mass percent (%) |
| Ceramide-like extract | 80 | 8.0 |
| Hydrogenated |
100 | 10.0 |
| Cholesterol | 3 | 0.3 |
| Glycerol | 350 | 35.0 |
| Butanediol | 150 | 15.0 |
| Pentanediol | 20 | 2.0 |
| Purified water | 297 | 29.7 |
2. Preparation method
(1) Heating 80g ceramide-like extract (prepared according to example 2 of patent CN201910667983.7, the same below) to 75 deg.C, adding 100g hydrogenated lecithin, 3g cholesterol, 150g butanediol, and 20g pentanediol, stirring at constant temperature of 70-75 deg.C, and optionally shearing and stirring (150rpm) to obtain uniform mixed solution;
(2) adding 350g of glycerol into the mixed solution at the constant temperature of 70-75 ℃, and uniformly stirring to prepare uniform mixed solution;
(3) adding 297g of purified water into the mixed solution at the constant temperature of 70-75 ℃, and uniformly stirring to prepare a mixed solution;
(4) carrying out constant temperature treatment on the mixed solution at 70-75 ℃, and carrying out circulation treatment through a high-pressure homogenizer for 6 times, wherein the former five times of homogenization pressure is 36-38 MPa, and the sixth time of homogenization circulation pressure is 80MPa, and homogenizing to obtain uniform and stable nano-liposome containing ceramide;
the obtained nanometer liposome containing ceramide is transparent, and particle size distribution is determined by particle size analyzer, and the result is shown in FIG. 1, wherein the particle size of liposome of nanometer liposome preparation is 60-420nm, and the average particle size is 179.2 nm; and are uniformly distributed.
Testing of stability: the nanometer liposome containing ceramide is stored in a shade place at room temperature for 24 months, and the appearance and the particle size of the liposome are not obviously changed.
Testing of Water dispersibility: the nano-liposome containing the ceramide is placed in water and stirred, so that the water dispersibility is good.
Example 2 preparation of nanoliposomes containing ceramide-like extracts
1. The composition of the liposome system is shown in table 2:
TABLE 2 nanoliposome system components containing ceramide-like extract
| Prescription composition | Quality (g) | Mass percent (%) |
| Ceramide-like extract | 50 | 5.0 |
| Hydrogenated lecithin | 50 | 5.0 |
| Soybean lecithin | 50 | 5.0 |
| Cholesterol | 2 | 0.2 |
| Glycerol | 300 | 30.0 |
| Butanediol | 130 | 13.0 |
| Pentanediol | 50 | 5.0 |
| Purified water | 368 | 36.8 |
2. Preparation method
(1) Heating 50g of ceramide-like extract to 80 ℃, adding 50g of hydrogenated lecithin, 50g of soybean phospholipid, 2g of cholesterol, 130g of butanediol and 50g of pentanediol, keeping the temperature at 70-75 ℃, stirring, and shearing and stirring (180rpm) if necessary to prepare a uniform mixed solution;
(2) adding 300g of glycerol into the mixed solution at the constant temperature of 70-75 ℃, and uniformly stirring to prepare uniform mixed solution;
(3) adding 368g of purified water into the mixed solution at the constant temperature of 70-75 ℃, and uniformly stirring to prepare a mixed solution;
(4) carrying out constant temperature of 70-75 ℃ on the mixed solution, carrying out circulation treatment by a high-pressure homogenizer for 5 times, wherein the homogenization pressure for the first four times is 38-40 MPa, and the homogenization circulation pressure for the 5 th time is 80MPa, and homogenizing to obtain uniform and stable nano-liposome containing the ceramide;
the obtained nanoliposome containing ceramide is semitransparent, and particle size distribution is determined by particle size analyzer, and as shown in FIG. 2, the liposome particle size of the nanoliposome preparation is 60-650nm, and the average particle size is 196.5 nm; and are uniformly distributed.
Testing of stability: the nanometer liposome containing ceramide is stored in a shade place at room temperature for 24 months, and the appearance and the particle size of the liposome are not obviously changed.
Testing of Water dispersibility: the nano-liposome containing the ceramide is placed in water and stirred, so that the water dispersibility is good.
EXAMPLE 3 preparation of nanoliposomes containing ceramide-like substances
1. The composition of the liposome system is shown in table 3:
TABLE 3 ceramide-like nanoliposome system components III
| Prescription composition | Mass (g) | Mass percent (%) |
| Ceramide-like extract | 30 | 3.0 |
| Hydrogenated lecithin | 40 | 4.0 |
| Liquid lecithin | 60 | 6.0 |
| |
5 | 0.5 |
| Glycerol | 350 | 35.0 |
| Butanediol | 140 | 14.0 |
| Pentanediol | 30 | 3.0 |
| Purified water | 345 | 34.5 |
2. Preparation method
(1) Heating 30g of ceramide-like extract to 80 ℃, adding 40g of hydrogenated lecithin, 60g of liquid lecithin, 5g of cholesterol, 140g of butanediol and 30g of pentanediol, keeping the temperature at 70-75 ℃, stirring, and shearing and stirring (180rpm) if necessary to prepare a uniform mixed solution;
(2) adding 350g of glycerol into the mixed solution at the constant temperature of 70-75 ℃, and uniformly stirring to prepare uniform mixed solution;
(3) adding 345g of purified water into the mixed solution at the constant temperature of 70-75 ℃, and uniformly stirring to prepare a mixed solution;
(4) carrying out constant temperature treatment on the mixed solution at 70-75 ℃, and carrying out circulation treatment through a high-pressure homogenizer for 6 times, wherein the former five times of homogenization pressure is 36-38 MPa, and the sixth time of homogenization circulation pressure is 80MPa, and homogenizing to obtain uniform and stable nano-liposome containing ceramide;
the obtained ceramide-containing nanoliposome is nearly transparent, and the particle size distribution of the nanoliposome preparation is measured by a particle size analyzer, and the results are shown in fig. 3, wherein the particle size of the liposome of the nanoliposome preparation is measured to be in the range of 60-550nm, and the average particle size is 183.1 nm; and are uniformly distributed.
Testing of stability: the nanometer liposome containing ceramide is stored in a shade place at room temperature for 24 months, and the appearance and the particle size of the liposome are not obviously changed.
Testing of Water dispersibility: the nano-liposome containing the ceramide is placed in water and stirred, so that the water dispersibility is good.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (10)
1. A nanoliposome containing a ceramide-like extract is characterized by comprising the following components in percentage by mass: 1-10% of ceramide extract, 0.0-3.0% of cholesterol, 3-15% of phospholipid, 25-50% of glycerol, 5-25% of polyalcohol and the balance of water.
2. The nanoliposome containing a ceramide-like extract as claimed in claim 1, which is characterized by comprising the following components in percentage by mass: 3-8% of ceramide extract, 0.2-0.5% of cholesterol, 10% of phospholipid, 30-35% of glycerol, 17-18% of polyalcohol and the balance of water.
3. The nanoliposome containing a ceramide-like extract as claimed in claim 1 or 2, wherein:
the phospholipid is at least one of hydrogenated phospholipid, liquid lecithin, soybean phospholipid, egg yolk lecithin and cephalin;
the polyalcohol is at least one of butanediol and pentanediol.
4. The nanoliposome containing a ceramide-like extract as claimed in claim 3, wherein:
the hydrogenated phospholipid is at least one of hydrogenated lecithin and hydrogenated soybean phospholipid;
the polyalcohol is a compound of butanediol and pentanediol.
5. The nanoliposome containing a ceramide-like extract as claimed in claim 1 or 2, wherein:
the particle size of the nano-liposome containing the ceramide-like extract is 50-700nm, the average particle size is less than 200nm, and the nano-liposome is uniformly distributed.
6. The method for preparing nanoliposomes containing a ceramide-like extract as claimed in any one of claims 1 to 5, which comprises the steps of:
(1) heating ceramide-like extract to 70-80 deg.C, adding phospholipid, cholesterol and polyalcohol, and stirring at 70-80 deg.C to obtain uniform mixed solution A;
(2) adding glycerol into the mixed solution A at the temperature of 70-80 ℃, and uniformly stirring to obtain a uniform mixed solution B;
(3) adding water into the mixed solution B at the temperature of 70-80 ℃, and uniformly stirring to obtain a uniform mixed solution C;
(4) and (3) keeping the temperature of the mixed solution C at 70-75 ℃, and homogenizing to obtain the nanoliposome containing the ceramide-like extract.
7. The method of claim 6, wherein:
the heating degree in the step (1) is 75-80 ℃;
the temperature in the step (2) is 70-75 ℃;
the temperature in the step (3) is 70-75 ℃.
8. The method of claim 6, wherein:
the homogenization conditions in the step (4) are as follows: homogenizing for 3-10 times at high pressure of 35-40MPa, and homogenizing for the last time at 80 MPa.
9. Use of the nanoliposome containing a ceramide-like extract as claimed in any one of claims 1 to 5 in the preparation of daily chemical products.
10. Use according to claim 9, characterized in that: the daily chemical product has at least one of the following effects: moisturizing, caring skin, whitening, and eliminating speckle.
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| CN115300444A (en) * | 2022-08-24 | 2022-11-08 | 深圳浦瑞健康科技有限公司 | Preparation method and application of propolis liposome |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101214198A (en) * | 2007-12-28 | 2008-07-09 | 清华大学 | Ceramide nano liposome preparations and preparation and application thereof |
| CN112280806A (en) * | 2019-07-23 | 2021-01-29 | 广东现代汉方科技有限公司 | Method for preparing ceramide by emulsification fermentation and application thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101214198A (en) * | 2007-12-28 | 2008-07-09 | 清华大学 | Ceramide nano liposome preparations and preparation and application thereof |
| CN112280806A (en) * | 2019-07-23 | 2021-01-29 | 广东现代汉方科技有限公司 | Method for preparing ceramide by emulsification fermentation and application thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115300444A (en) * | 2022-08-24 | 2022-11-08 | 深圳浦瑞健康科技有限公司 | Preparation method and application of propolis liposome |
| CN115300444B (en) * | 2022-08-24 | 2023-08-04 | 深圳浦瑞健康科技有限公司 | Preparation method and application of propolis liposome |
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