CN114711238B - Plant growth regulator composition for improving anthocyanin content of succulent flower leaves - Google Patents
Plant growth regulator composition for improving anthocyanin content of succulent flower leaves Download PDFInfo
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- 229930002877 anthocyanin Natural products 0.000 title claims abstract description 35
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- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a plant growth regulator composition for improving anthocyanin content in succulent flower leaves, and belongs to the technical field of quality and efficiency improvement of horticultural products. The plant growth regulator composition consists of 1000-3000 times diluted brassinolide and 2000-6000 times diluted diethyl aminoethyl hexanoate in a volume ratio of 1. The plant growth regulator composition can obviously improve the anthocyanin content in the leaves of the succulent flowers and improve the ornamental quality of the succulent flowers; meanwhile, the healthy growth of succulent flowers can be promoted, the stress resistance of succulent flower leaves is enhanced, and the purposes of increasing color and improving quality are achieved.
Description
Technical Field
The invention relates to the technical field of quality improvement and synergism of horticultural products, in particular to a plant growth regulator composition for improving anthocyanin content in succulent flower leaves.
Background
Succulent flowers are succulent plants with leaf water content of more than 95%, belonging to Crassulaceae, ainsliaceae, cactaceae, liliaceae, etc. The thick and juicy leaves store a large amount of water and nutrients, and the more durable vital characteristics of the leaves are ensured to be maintained under the stress of drought, strong light and other adversities. The various flowers are hot in the flower market in recent years due to various shapes, smallness and gorgeous colors.
Anthocyanins (Anthocyanin) are flavonoid secondary metabolites widely found in plants. The higher the anthocyanin content is, the more vivid the color of the leaves, flowers or fruits of the plant is, and the higher the ornamental value is. The vividness and compactness of the leaves are the main evaluation indexes of the market value of the current succulent flowers. Therefore, it is important to improve the color degree and uniformity of the fleshy leaves for the commercial value and economic benefit thereof.
Research shows that under general conditions, anthocyanin is converted from chlorophyll, and the contents of two pigments show a correlation dynamic change relationship [ Hongbai, wuyuwei, song Xiang, li Meng Ling, dailan, molecular mechanism for regulating and controlling anthocyanin biosynthesis of horticultural crops by illumination [ J ]. Hill. Schedule, 2021,48 (10): 1983-2000 ]. The color development of the succulent plant leaves is influenced by both heredity and environment, and stresses such as illumination, temperature, drought and the like are important environmental factors causing succulence and hyperchromia. Li Zunyo et al showed that the higher the illumination intensity, the more abundant the color of the ` ruby ` succulent leaves [ Li Zunyo, liu Jiu, wang hong Yu, lushu Meng, jing. However, the research result only aims at the illumination intensity in the natural environment condition, and is greatly and unstably influenced by the environment. In a large succulent seedling raising field, the degree of pigmentation can be improved to different degrees by artificial light supplement, but the cost is high and the feasibility is low.
The succulent flowers have strong drought tolerance, and the drought stress with different intensities can increase the color of the succulent leaves to different degrees, but the severe drought also affects the luster and normal growth of the succulent leaves. Therefore, the exogenous substance capable of rapidly promoting the coloring of the succulent leaves and shortening the coloring process is provided without influencing the normal growth and development of the succulent leaves, so that the coloring time is greatly shortened, the production cost is saved, and the production benefit is improved.
Sodium nitrophenolate, S-abscisic acid, brassinolide, diethyl aminoethyl hexanoate and the like are all healthy and environment-friendly plant growth regulators, and are widely applied to the aspects of fruit coloring, quality improvement and resistance enhancement of horticultural crops (grapes, apples, tomatoes and the like), but the research on improving the color of fleshy flowers is not reported at present.
Disclosure of Invention
In view of the above prior art, the present invention aims to provide a plant growth regulator composition for increasing anthocyanin content in leaves of succulent flowers. The plant growth regulator composition can obviously improve the anthocyanin content in the leaves of the succulent flowers and improve the ornamental quality of the succulent flowers; meanwhile, the healthy growth of succulent flowers can be ensured, and the purposes of color enhancement and quality improvement are achieved.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect of the invention, a plant growth regulator composition is provided, which consists of brassinolide and diethyl aminoethyl hexanoate.
Preferably, the plant growth regulator composition consists of 1000-3000 times diluted brassinolide and 2000-6000 times diluted diethyl aminoethyl hexanoate according to a volume ratio of 1.
Preferably, the plant growth regulator composition consists of 1000 times diluted brassinolide and 2000 times diluted diethyl aminoethyl hexanoate in a volume ratio of 1.
Preferably, the pesticide registration number of the brassinolide is PD20094241; the pesticide registration number of the diethyl aminoethyl hexanoate is PD20130757.
In a second aspect of the present invention, there is provided a use of the above plant growth regulator composition in (1) or (2):
(1) The anthocyanin content of the succulent flower leaves is improved;
(2) The preparation for improving the anthocyanin content of the succulent flower leaves is prepared.
In a third aspect of the invention, the invention provides a preparation for increasing anthocyanin content in succulent flower leaves, which comprises the plant growth regulator composition and an agriculturally and pharmaceutically acceptable auxiliary material.
Preferably, the agriculturally pharmaceutically acceptable excipients include: emulsifiers (e.g., sorbitan polyoxyethylene ether, polyethylene glycol aryl diphenyl ether, castor oil polyoxyethylene ether), pH stabilizers (e.g., trisodium citrate, tripotassium phosphate), fillers (e.g., glucose, lactose, anhydrous magnesium sulfate), and the like.
Preferably, the preparation is in the form of aqua, wettable powder, water dispersant, oil suspending agent, microemulsion or aqueous emulsion.
In a fourth aspect of the invention, a method for increasing anthocyanin content in succulent flower leaves is provided, which comprises the following steps: spraying the plant growth regulator composition on the leaves of the succulent flowers.
In the above method, preferably, the number of spraying of the plant growth regulator composition is 2, and the interval between the two spraying is 30 days.
Preferably, the varieties of the succulent flowers are Prussian, C419, C452 and sugar.
The invention has the beneficial effects that:
according to the invention, the plant growth regulator is combined by the brassinolide and the diethyl aminoethyl hexanoate, and the combination of the plant growth regulator and the diethyl aminoethyl hexanoate can quickly and effectively improve the anthocyanin content and the color development effect of the leaves of the succulent flowers, and the plant growth regulator has more uniform and bright color compared with the single plant growth regulator which is used independently; has obvious synergistic effect in improving the anthocyanin content of the succulent flower leaves.
Drawings
FIG. 1: color generation effect of different concentration combinations on 'Prola' varieties.
FIG. 2: color development effect of different concentrations combined on 'C419' variety.
FIG. 3: color development effect of different concentrations combined on 'C452' variety.
FIG. 4 is a schematic view of: color development effect of different concentrations combined on 'sugar pulling' varieties.
FIG. 5 is a schematic view of: relative content of anthocyanins in "Prola" fleshy leaves.
FIG. 6: relative content of anthocyanin in ` C419 ` fleshy leaves.
FIG. 7 is a schematic view of: relative content of anthocyanin in ` C452 ` fleshy leaves.
FIG. 8: relative content of anthocyanin in ` Lam ` succulent leaves.
The specific implementation mode is as follows:
it should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
As mentioned above, the compound sodium nitrophenolate, the S-abscisic acid, the brassinolide and the diethyl aminoethyl hexanoate are all healthy and environment-friendly plant growth regulators, but the research on the improvement of the color enhancement of the succulent flowers is not reported yet.
Based on the method, the coloring effect of the succulent flower leaves is researched by spraying exogenous plant growth regulator combinations of different types and concentrations, so that a convenient way is provided for improving the commercial performance of the succulent flower leaves.
It is known that the combination of active ingredients of different plant growth regulators after mixing them is usually shown as synergistic, additive or antagonistic, due to the differences in composition, structure, physicochemical properties of the active ingredients. Wherein:
the chemical components of the compound sodium nitrophenolate are 5-nitroguaiacol sodium, o-nitrophenol sodium and p-nitrophenol sodium; can effectively promote germination, rooting, dormancy release, growth and development, accelerate plant metabolism, improve crop yield and quality, prevent flower and fruit dropping, enhance the capabilities of disease resistance, insect resistance, drought resistance, lodging resistance and the like, and has the advantages of wide applicability, high efficiency and micro toxicity.
Brassinolide is a green environment-friendly hormone plant growth regulator, and the structural formula of the brassinolide is shown as follows:
the brassinolide can promote the growth of root systems, enhance the photosynthesis of plants, and enable the plants to have bright color and thick leaves.
Diethyl aminoethyl hexanoate is a plant growth regulator with broad spectrum and breakthrough, and the structural formula of the diethyl aminoethyl hexanoate is shown as follows:
the diethyl aminoethyl hexanoate can promote the division and growth of plant cells to accelerate the development of root systems, enhance photosynthesis and improve the content of chlorophyll.
The S-abscisic acid is a natural plant growth regulator, and can promote the coordinated growth of plants and enhance the stress resistance of the plants. Can improve the synthesis and accumulation of anthocyanins such as grape and apple, and promote fruit color increase and precocity.
The invention inspects the influence of the 4 plant growth regulators and the combination thereof on the anthocyanin content of the succulent flower leaves, and the result shows that: the brassinolide and diethyl aminoethyl hexanoate are combined for use, so that the brassinolide-diethyl aminoethyl hexanoate has a remarkable synergistic effect on the aspect of improving the anthocyanin content of the leaves of the succulent flowers, and the invention is provided.
In order to make the technical solutions of the present application more clearly understood by those skilled in the art, the technical solutions of the present application will be described in detail below with reference to specific embodiments. If the experimental conditions not specified in the examples are specified, they are generally according to the conventional conditions or according to the conditions recommended by the reagents company; reagents, consumables, and the like used in the following examples are commercially available unless otherwise specified. Wherein:
sodium nitrophenolate, produced by Zhangzhou chemical industry Co., ltd. The content of the total effective components is 1.8 percent, the content of the o-nitrophenol sodium is 0.6 percent, the content of the p-nitrophenol sodium is 0.9 percent, and the content of the 5-nitro-o-methoxy-phenol sodium is 0.3 percent; the preparation is water solution. The official registration number of the pesticide is PD20096552.
Brassinolide, produced by ericco chemical limited. The content of total effective components is 0.01%; the formulation is missible oil. The pesticide registration number is PD20094241.
Diethyl aminoethyl hexanoate, produced by Biotech limited of Haoyao, shanxi, with an active ingredient content of 8%; the preparation is water solution. The pesticide registration number is PD20130757.
S-abscisic acid, produced by Szechwan python Bothon Tech Limited liability company (Jinmeihong brand S-abscisic acid), with an effective component content of 5%; the dosage form is soluble solution. The pesticide registration certificate number is PD20152355.
Example 1:
1. and (3) test treatment:
4 succulent varieties (Prava, C419, C452, rasa) were tested in combinations of different ratios, with different concentrations of toner diluted in water, with different concentrations of the individual colorants being set simultaneously (Table 1). 100 plants with consistent growth vigor are selected for each variety under each combination, and are sprayed twice during the 3-month test period (the first spraying of 2021.10.09), wherein the spraying part is fleshy flower leaves, and the even wetting of the whole fleshy stems and leaves is preferred. The spraying time interval is 30 days, the observation time is 30 days later, and the anthocyanin content in the leaves is sampled and determined. The diurnal temperature difference was maintained between 10 ℃ and 12 ℃ during the test. The other management measures are consistent with the production.
TABLE 1 colorant treatment of different kinds and concentrations
| Treatment of | Compound sodium nitrophenolate (FN) | Brassinolide (YZ) | S-abscisic acid (SY) | Diethyl aminoethyl hexanoate (AZ) |
| |
0 | 0 | 0 | 0 |
| 1 | 4000 | 0 | 0 | 0 |
| 2 | 6000 | 0 | 0 | 0 |
| 3 | 0 | 1000 | 0 | 0 |
| 4 | 0 | 2000 | 0 | 0 |
| 5 | 0 | 3000 | 0 | 0 |
| 6 | 0 | 0 | 4000 | 0 |
| 7 | 0 | 0 | 6000 | 0 |
| 8 | 0 | 0 | 0 | 2000 |
| 9 | 0 | 0 | 0 | 4000 |
| 10 | 0 | 0 | 0 | 6000 |
| 11 | 4000 | 2000 | 0 | 0 |
| 12 | 6000 | 3000 | 0 | 0 |
| 13 | 4000 | 0 | 4000 | 0 |
| 14 | 6000 | 0 | 6000 | 0 |
| 15 | 4000 | 0 | 0 | 4000 |
| 16 | 6000 | 0 | 0 | 6000 |
| 17 | 0 | 2000 | 4000 | 0 |
| 18 | 0 | 3000 | 6000 | 0 |
| 19 | 0 | 1000 | 0 | 2000 |
| 20 | 0 | 2000 | 0 | 4000 |
| 21 | 0 | 3000 | 0 | 6000 |
| 22 | 0 | 0 | 4000 | 4000 |
| 23 | 0 | 0 | 6000 | 6000 |
Note: the corresponding values for the different colorants in table 1 indicate the dilution factor of the colorant, with 0 indicating that the colorant was not used; the combination of the coloring agents refers to that the coloring agents diluted according to different proportions are compounded according to the volume ratio of 1.
The anthocyanin content was determined by organic solvent extraction [ references: influence of plum Zygun, liu He, liu Jade, wang Hongyu, lushu Meng, oryza chinensis on color development and canopy width of the fleshy leaves of ruby [ J ] modern horticulture 2020,43 (07): 26-28 ].
Relative content of anthocyanins = OD530/0.1W;
in the formula: OD530 is the absorbance value at the wavelength of 530 nm; w is the leaf weight in g.
2. And (4) counting results:
the 4 varieties of succulent adult plants treated by the plant growth agent combination are investigated, and the hyperchromic effect is evaluated from the color generation of the leaves and the content of anthocyanin.
Wherein the coloring effect of the leaves is respectively shown in figures 1-4. The results of the anthocyanin content assay for the different treatments are shown in table 2 and fig. 5-8.
TABLE 2 anthocyanin content in succulent flower leaves treated with different colorant combinations
The above results show that: by spraying the proper growth regulator combination, the anthocyanin content and the color development effect in the leaves of the succulent flowers can be quickly and effectively improved, and the plant color is more uniform and bright than that of a plant with a contrast and a single growth regulator. However, because there may be reaction between plant growth regulators, if the combination of plant growth regulators is not properly selected, the plant growth regulators will have opposite effects, and the coloring effect on the fleshy flower leaves is worse than that of a single plant growth regulator.
The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made to the present application by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims (4)
1. Use of a plant growth regulator composition in (1) or (2) below:
(1) The anthocyanin content of the succulent flower leaves is improved;
(2) Preparing a preparation for improving the anthocyanin content of the succulent flower leaves;
the plant growth regulator composition consists of 1000-time diluted brassinolide and 2000-time diluted diethyl aminoethyl hexanoate in a volume ratio of 1;
the total effective component content of the brassinolide before dilution is 0.01 percent; the content of active ingredients of the diethyl aminoethyl hexanoate before dilution is 8%.
2. The use of claim 1, wherein the brassinolide has a pesticide accession number of PD20094241; the pesticide registration number of the diethyl aminoethyl hexanoate is PD20130757.
3. A method for improving anthocyanin content in succulent flower leaves is characterized by comprising the following steps: spraying a plant growth regulator composition on the leaves of the succulent flowers;
the plant growth regulator composition consists of 1000-time diluted brassinolide and 2000-time diluted diethyl aminoethyl hexanoate in a volume ratio of 1; the total effective component content of the brassinolide before dilution is 0.01 percent; the content of active ingredients of the diethyl aminoethyl hexanoate before dilution is 8%;
the spraying frequency of the plant growth regulator composition is 2 times, and the interval of the two spraying is 30 days.
4. The method of claim 3, wherein the cultivars of succulent flowers are Prussian, C419, C452 and Raglas.
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