CN114657239B - 用于多重pcr二代测序的引物组合、试剂盒及建库方法 - Google Patents
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Abstract
本发明涉及分子生物学检测领域，具体涉及一种用于多重PCR二代测序的引物组合、试剂盒及建库方法，包括提取样本DNA，基于提取的样本DNA，利用引物组合配置第一步PCR扩增体系，进行第一步PCR扩增并纯化，基于第一步PCR扩增并纯化后的纯化产物进行第二次PCR扩增并纯化，对第二次PCR扩增并纯化后的纯化产物进行电泳鉴定后进行测序分析；本发明可以有效解决Ampliseq试剂盒存在的时间成本和金钱成本高的问题，对于客户定制化的panel设计生产周期只需要两周，并且可以极大的降低单个样本建库的成本，且在IonTorrent和Illumina测序平台都通用。
Description
技术领域
本发明涉及分子生物学检测领域，具体涉及一种用于多重PCR二代测序的引物组合、试剂盒及建库方法。
背景技术
二代测序由于其超高的测序能力在科研和临床中具有极为重要的应用。二代测序技术也称深度测序、大规模平行测序，核心思想是边合成边测序(Sequencing bySynthesis)，即通过捕捉新合成的末端的标记来确定DNA的序列，现有的技术平台主要有Roche/454FLX、Illumina/Solexa Genome Analyzer和Applied Biosystems SOLIDsystem。这三个技术平台各有优点，454FLX的测序片段比较长，高质量的读长能达到400bp；Solexa测序性价比最高，不仅机器的售价比其他两种低，而且运行成本也低，在数据量相同的情况下，成本只有454测序的1/10；SOLID测序的准确度高，原始碱基数据的准确度大于99.94％，而在15×覆盖率时的准确度可以达到99.999％。
Illumina/Solexa Genome Analyzer测序的基本原理是边合成变测序。在Sanger等测序方法的基础上，通过技术创新，用不同颜色的荧光标记四种不同的dNTP，当DNA聚合酶合成互补链时，每添加一种dNTP就会释放出不同的荧光，根据捕捉的荧光信号并经过特定的计算机软件处理，从而获得待测DNA的序列信息。
二代测序的一般流程如下：1)文库制备，将DNA用雾化或超声波随机片段化成几百碱基或更短的小片段。用聚合酶和外切核酸酶把DNA片段切成平末端，紧接着磷酸化并增加一个核苷酸黏性末端。然后将测序接头与片段连接；2)簇的创建，将模板分子加入芯片用于产生克隆簇和测序循环。芯片有8个纵向泳道的硅基片。每个泳道内芯片表面有无数的被固定的单链接头。上述步骤得到的带接头的DNA片段变性成单链后与测序通道上的接头引物结合形成桥状结构，以供后续的预扩增使用。通过不断循环获得上百万条成簇分布的双链待测片段；3)测序，DNA聚合酶结合荧光可逆终止子，荧光标记簇成像，在下一个循环开始
前将结合的核苷酸剪切并分解；4)数据分析，测序得到的原始数据是长度为几十个碱基的序列，要通过生物信息学工具将这些短的序列组装成长的Contigs甚至是整个基因组的框架，或者把这些序列比对到已有的基因组或者相近物种基因组序列上，进一步分析得到有生物学意义的结果。
聚合酶链反应(即PCR)是一种广泛运用于分子遗传和诊断的技术，用于放大扩增特定的DNA片段，可看作是生物体外的特殊DNA复制，可分析任何短的DNA序列，PCR的最大特点，是能将微量的DNA大幅增加，即使样品中只含有低水平的DNA。PCR技术可用于扩增分析用的DNA或RNA选定片段。
目前市场上最成功的商业化多重PCR建库技术是Thermo Fisher公司的Ampliseq技术，该技术可以在同一孔反应中完成上千对引物的扩增，使得多个靶标区域的富集可以通过一次PCR反应完成，随后加上Ion Torrent测序平台的通用接头建好文库用于二代测序。然而，这个产品存在最大的缺陷是客户定制化的panel设计生产周期较长，需要两到三个月，严重的增加了时间成本；并且每个样本的建库费用高达1000-2000元，致使其经济成本极高；另外该试剂盒只适用于Ion Torrent测序平台，受平台因素影响大，亦不利于测序费用的降低。
发明内容
针对上述现有技术的不足，本发明旨在提供一种用于多重PCR二代测序的引物组合、试剂盒及建库方法，以解决现有建库技术时间成本及金钱成本高的问题。
为了解决上述问题，本发明采用了如下的技术方案：
设计一种用于多重PCR的二代测序的引物组合，包括：如SEQ ID NO:1-SEQ ID NO:806所示核苷酸序列；
基于设计的引物组合涉及试剂盒以及反应体系进行建库，包括：
1)、提取样本DNA；
2)、基于提取的样本DNA，配置第一步PCR扩增体系，进行第一步PCR扩增并纯化，
3)、基于第一步PCR扩增并纯化后的纯化产物进行第二次PCR扩增并纯化；
4)、对第二次PCR扩增并纯化后的纯化产物进行电泳鉴定后进行测序分析。
本发明的有益效果在于：本发明可以有效的解决Ampliseq试剂盒存在的问题，对于客户定制化的panel设计生产周期只需要两周，并且可以极大的降低单个样本建库的成本，且在IonTorrent和Illumina测序平台都通用。
具体实施方式
下面结合具体实施例对本发明作进一步的详细说明。
需要说明的是，这些实施例仅用于说明本发明，而不是对本发明的限制，在本发明的构思前提下本方法的简单改进，都属于本发明要求保护的范围。
实施例一
特异性引物设计
本发明采用表1中的特异性引物进行建库。
表1引物序列
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实施例二
DNA制备
按医疗常规操作采集外周静脉血，或组织或鼻咽拭子样本等，使用核酸提取试剂盒提取DNA。
实施例三
第一步PCR扩增
将403对引物(共806管引物)，每管引物等体积(根据样本例数计算)混匀，为Primer Mix。
每例DNA样本，制备PCR体系(总反应体系为50μL)，分别为PCR反应液、引物混合物Primer Mix、及DNA样本组成：
PCR反应液由25μlPlatinum Multiplex PCR Master Mix 2×(赛默飞)、5μl GCEnhancer(赛默飞)混合；
引物混合物Primer Mix 15μl；
DNA样本5μl。
充分混匀反应体系，短暂离心，进行扩增反应。PCR扩增条件如下：98℃,3分钟；96℃45秒，80℃30秒，54℃3分钟，68℃30秒,共45个循环。
实施例四
PCR产物纯化
磁珠充分混匀，取0.8倍体积的磁珠加入；涡旋震荡，室温放置5min，使DNA充分吸附在磁珠上；短暂离心，将离心管放在磁力架上，静置5min；待溶液清亮，用枪头小心吸弃上清液，避免吸到磁珠；加入180μL当天配置的80％乙醇漂洗，静置30sec；吸弃乙醇；短暂离心，将离心管放置在磁力架上，用10μL枪头彻底将液体吸净；室温干燥5min使乙醇尽量挥发干净；加入25μL ddH2O，涡旋混匀，室温静置5min；短暂离心，将离心管放在磁力架上5min；转移23μL DNA溶液至干净的标记好的PCR管中。
实施例五
第二步PCR扩增
制备PCR体系(总反应体系为50μL)，分别为PCR反应液、PE1.0、Barcode及上步纯化产物组成：
PCR反应液指25μl 2×KAPA HiFi Hotstart Ready Mix(KAPA)；
1μl PE1.0；
1μl Barcode；
23μl上步纯化产物。
充分混匀反应体系，短暂离心，进行扩增反应。PCR扩增条件如下：98℃,3分钟；98℃20秒，65℃30秒，72℃20秒，共15个循环；68℃10分钟。
实施例五
PCR产物纯化
磁珠充分混匀，取1.8倍PCR产物体积的磁珠加入(例如：50μL PCR产物加入90μL磁珠)；涡旋震荡，室温放置5min，使DNA充分吸附在磁珠上；短暂离心，将离心管放在磁力架上，静置5min；待溶液清亮，用枪头小心吸弃上清液，避免吸到磁珠；加入180μL当天配置的80％乙醇漂洗，静置30sec；吸弃乙醇；短暂离心，将离心管放置在磁力架上，用10μL枪头彻底将液体吸净；室温干燥5min使乙醇尽量挥发干净；加入20μL ddH2O，涡旋混匀，室温静置5min；短暂离心，将离心管放在磁力架上5min；转移18μL DNA溶液至干净的标记好的离心管中；Qubit测定浓度，将文库稀释至1.5ng/μL，交给测序组测序。测序数据量每例样本1.5G。
实施例六
电泳鉴定
每个样品取5uL，进行2.5％的琼脂糖电泳，观察是否有条带，以及条带长度是否在100-200bp之间。
实施例七
结果分析
方法建库，HiSeq2000测序平台PE 2*150测序，单个样本取100Mb数据，上靶率98％，平均深度为985×，98.9％的位点测序深度在10×以上。
最后说明的是，以上实施例仅用以说明本发明的技术方案而非限制，尽管通过参照本发明的优选实施例已经对本发明进行了描述，但本领域的普通技术人员应当理解，可以在形式上和细节上对其作出各种各样的改变，而不偏离所附权利要求书所限定的本发明的精神和范围。
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<212> DNA
<213> artificial synthesis
<400> 9
ccctacacga cgctcttccg atctacttgt caagtgattt acaagtattt t 51
<210> 10
<211> 56
<212> DNA
<213> artificial synthesis
<400> 10
gttcagacgt gtgctcttcc gatctctaag tgcctgatct attaaaggaa aaatta 56
<210> 11
<211> 42
<212> DNA
<213> artificial synthesis
<400> 11
ccctacacga cgctcttccg atctccagta tttaaattcc ta 42
<210> 12
<211> 56
<212> DNA
<213> artificial synthesis
<400> 12
gttcagacgt gtgctcttcc gatctcactt ggtcactcta cttataactt tcatat 56
<210> 13
<211> 47
<212> DNA
<213> artificial synthesis
<400> 13
ccctacacga cgctcttccg atctgcaaca ctagaacaga gataatg 47
<210> 14
<211> 44
<212> DNA
<213> artificial synthesis
<400> 14
gttcagacgt gtgctcttcc gatctatgga atctactgaa aaaa 44
<210> 15
<211> 46
<212> DNA
<213> artificial synthesis
<400> 15
ccctacacga cgctcttccg atctgcaaag gaatgaagac tccaaa 46
<210> 16
<211> 46
<212> DNA
<213> artificial synthesis
<400> 16
gttcagacgt gtgctcttcc gatctatgaa aaattccaca taaaat 46
<210> 17
<211> 42
<212> DNA
<213> artificial synthesis
<400> 17
ccctacacga cgctcttccg atctgaggaa actctcataa ac 42
<210> 18
<211> 53
<212> DNA
<213> artificial synthesis
<400> 18
gttcagacgt gtgctcttcc gatcttcagg cacctgaagg actgggtgaa tag 53
<210> 19
<211> 48
<212> DNA
<213> artificial synthesis
<400> 19
ccctacacga cgctcttccg atctcagaaa ggcctaaaat ttctcatt 48
<210> 20
<211> 53
<212> DNA
<213> artificial synthesis
<400> 20
gttcagacgt gtgctcttcc gatctatttg gaaaagtagt cccctaagtc att 53
<210> 21
<211> 41
<212> DNA
<213> artificial synthesis
<400> 21
ccctacacga cgctcttccg atcttctttc caatgcatta t 41
<210> 22
<211> 41
<212> DNA
<213> artificial synthesis
<400> 22
gttcagacgt gtgctcttcc gatctcaggt agagcaagaa t 41
<210> 23
<211> 49
<212> DNA
<213> artificial synthesis
<400> 23
ccctacacga cgctcttccg atctagctgt ccctggattc atcttcccc 49
<210> 24
<211> 53
<212> DNA
<213> artificial synthesis
<400> 24
gttcagacgt gtgctcttcc gatcttagga aagaaattct ggatttcaac atg 53
<210> 25
<211> 43
<212> DNA
<213> artificial synthesis
<400> 25
ccctacacga cgctcttccg atctgcttct agaggttaaa tta 43
<210> 26
<211> 48
<212> DNA
<213> artificial synthesis
<400> 26
gttcagacgt gtgctcttcc gatctttcac agcacttctt ctatgctg 48
<210> 27
<211> 41
<212> DNA
<213> artificial synthesis
<400> 27
ccctacacga cgctcttccg atcttttggt tggtgccaac a 41
<210> 28
<211> 42
<212> DNA
<213> artificial synthesis
<400> 28
gttcagacgt gtgctcttcc gatctgtcag tgaattagca gg 42
<210> 29
<211> 51
<212> DNA
<213> artificial synthesis
<400> 29
ccctacacga cgctcttccg atctttatga aactcaaggg tcataagaat t 51
<210> 30
<211> 44
<212> DNA
<213> artificial synthesis
<400> 30
gttcagacgt gtgctcttcc gatctaaaga ctaaacaaaa ctat 44
<210> 31
<211> 55
<212> DNA
<213> artificial synthesis
<400> 31
ccctacacga cgctcttccg atctcctcac atttcaaaac acaatcatgc cttcc 55
<210> 32
<211> 54
<212> DNA
<213> artificial synthesis
<400> 32
gttcagacgt gtgctcttcc gatctaacta gattttgatt tcacagactt atag 54
<210> 33
<211> 43
<212> DNA
<213> artificial synthesis
<400> 33
ccctacacga cgctcttccg atctttccgt ctgcaagctg gat 43
<210> 34
<211> 56
<212> DNA
<213> artificial synthesis
<400> 34
gttcagacgt gtgctcttcc gatctctgtg gttgagctgg gacttccatc ttctcc 56
<210> 35
<211> 48
<212> DNA
<213> artificial synthesis
<400> 35
ccctacacga cgctcttccg atctccattt gttgaagaga acattttt 48
<210> 36
<211> 51
<212> DNA
<213> artificial synthesis
<400> 36
gttcagacgt gtgctcttcc gatctaaaca atatggcact gtcatagaaa a 51
<210> 37
<211> 52
<212> DNA
<213> artificial synthesis
<400> 37
ccctacacga cgctcttccg atctgctgag ttccagataa tttcaggttt ct 52
<210> 38
<211> 41
<212> DNA
<213> artificial synthesis
<400> 38
gttcagacgt gtgctcttcc gatctctctg tgtccagttg t 41
<210> 39
<211> 53
<212> DNA
<213> artificial synthesis
<400> 39
ccctacacga cgctcttccg atcttggacc ctaacataaa ctatggactt ggg 53
<210> 40
<211> 52
<212> DNA
<213> artificial synthesis
<400> 40
gttcagacgt gtgctcttcc gatctataat gaacagaaga tacagagatt tc 52
<210> 41
<211> 43
<212> DNA
<213> artificial synthesis
<400> 41
ccctacacga cgctcttccg atctgcagtt agagcatgca gaa 43
<210> 42
<211> 43
<212> DNA
<213> artificial synthesis
<400> 42
gttcagacgt gtgctcttcc gatctcatgg tctcaagatg taa 43
<210> 43
<211> 53
<212> DNA
<213> artificial synthesis
<400> 43
ccctacacga cgctcttccg atctattttt ccaacagtat gtgcgcactt cat 53
<210> 44
<211> 50
<212> DNA
<213> artificial synthesis
<400> 44
gttcagacgt gtgctcttcc gatctttcac catcttatcg ggtgcggctt 50
<210> 45
<211> 50
<212> DNA
<213> artificial synthesis
<400> 45
ccctacacga cgctcttccg atctcaggag ggcttacgtt tgaagtctgt 50
<210> 46
<211> 44
<212> DNA
<213> artificial synthesis
<400> 46
gttcagacgt gtgctcttcc gatcttttgg tcccgccacc ctgc 44
<210> 47
<211> 54
<212> DNA
<213> artificial synthesis
<400> 47
ccctacacga cgctcttccg atcttgcttg tgaggtgagc tgcgagctga aaca 54
<210> 48
<211> 44
<212> DNA
<213> artificial synthesis
<400> 48
gttcagacgt gtgctcttcc gatctttcaa tcttcatatc gcac 44
<210> 49
<211> 55
<212> DNA
<213> artificial synthesis
<400> 49
ccctacacga cgctcttccg atctctctgc ccgtggaatg tacatggatg tttgg 55
<210> 50
<211> 52
<212> DNA
<213> artificial synthesis
<400> 50
gttcagacgt gtgctcttcc gatcttgacc acacacatgc cccgtgaagc ag 52
<210> 51
<211> 53
<212> DNA
<213> artificial synthesis
<400> 51
ccctacacga cgctcttccg atctaaagag tttattatta aaaggagcat atg 53
<210> 52
<211> 41
<212> DNA
<213> artificial synthesis
<400> 52
gttcagacgt gtgctcttcc gatctctaaa ccagtttatt a 41
<210> 53
<211> 53
<212> DNA
<213> artificial synthesis
<400> 53
ccctacacga cgctcttccg atctttctct actctctttg ccttctttga cct 53
<210> 54
<211> 48
<212> DNA
<213> artificial synthesis
<400> 54
gttcagacgt gtgctcttcc gatctttcta aattactgaa gttatctt 48
<210> 55
<211> 40
<212> DNA
<213> artificial synthesis
<400> 55
ccctacacga cgctcttccg atctgatgaa cataggagaa 40
<210> 56
<211> 53
<212> DNA
<213> artificial synthesis
<400> 56
gttcagacgt gtgctcttcc gatctcagta gtggtagatt gagaccatct cat 53
<210> 57
<211> 44
<212> DNA
<213> artificial synthesis
<400> 57
ccctacacga cgctcttccg atctatgtta gcagcagaac aaag 44
<210> 58
<211> 44
<212> DNA
<213> artificial synthesis
<400> 58
gttcagacgt gtgctcttcc gatctctcca gatgtttctg ttgg 44
<210> 59
<211> 43
<212> DNA
<213> artificial synthesis
<400> 59
ccctacacga cgctcttccg atcttattgc acttcaaaag tat 43
<210> 60
<211> 43
<212> DNA
<213> artificial synthesis
<400> 60
gttcagacgt gtgctcttcc gatctccaca caaaatttat ttt 43
<210> 61
<211> 42
<212> DNA
<213> artificial synthesis
<400> 61
ccctacacga cgctcttccg atctatgcct tttagcaaaa tt 42
<210> 62
<211> 48
<212> DNA
<213> artificial synthesis
<400> 62
gttcagacgt gtgctcttcc gatctcaact ccactcccct gggggtgg 48
<210> 63
<211> 42
<212> DNA
<213> artificial synthesis
<400> 63
ccctacacga cgctcttccg atctgccagg aaaaacgatg aa 42
<210> 64
<211> 52
<212> DNA
<213> artificial synthesis
<400> 64
gttcagacgt gtgctcttcc gatcttgttt tggcatcagt gttatgggat gt 52
<210> 65
<211> 43
<212> DNA
<213> artificial synthesis
<400> 65
ccctacacga cgctcttccg atctccctgt aacttggaag ttc 43
<210> 66
<211> 54
<212> DNA
<213> artificial synthesis
<400> 66
gttcagacgt gtgctcttcc gatctcctat tggtattcag aggcggtatc ctct 54
<210> 67
<211> 40
<212> DNA
<213> artificial synthesis
<400> 67
ccctacacga cgctcttccg atctctctgt agctttctta 40
<210> 68
<211> 49
<212> DNA
<213> artificial synthesis
<400> 68
gttcagacgt gtgctcttcc gatctccagg gcatcaaagt caaactcag 49
<210> 69
<211> 40
<212> DNA
<213> artificial synthesis
<400> 69
ccctacacga cgctcttccg atcttctctg gcgattgctg 40
<210> 70
<211> 52
<212> DNA
<213> artificial synthesis
<400> 70
gttcagacgt gtgctcttcc gatcttccta tcccggaccc tgcaggtggg tg 52
<210> 71
<211> 49
<212> DNA
<213> artificial synthesis
<400> 71
ccctacacga cgctcttccg atcttaaagt ttagactcta gagtccctc 49
<210> 72
<211> 54
<212> DNA
<213> artificial synthesis
<400> 72
gttcagacgt gtgctcttcc gatcttaggt atcccagaaa gagattctag gatg 54
<210> 73
<211> 42
<212> DNA
<213> artificial synthesis
<400> 73
ccctacacga cgctcttccg atctaacttt ttctttctct at 42
<210> 74
<211> 52
<212> DNA
<213> artificial synthesis
<400> 74
gttcagacgt gtgctcttcc gatctcgtta ttgcaaacgt gaagtcttaa ga 52
<210> 75
<211> 45
<212> DNA
<213> artificial synthesis
<400> 75
ccctacacga cgctcttccg atctcaatat ctgtgattta ataca 45
<210> 76
<211> 44
<212> DNA
<213> artificial synthesis
<400> 76
gttcagacgt gtgctcttcc gatcttaatc caaggcaaca caat 44
<210> 77
<211> 49
<212> DNA
<213> artificial synthesis
<400> 77
ccctacacga cgctcttccg atcttgagaa tcagggaaca ggctagctc 49
<210> 78
<211> 47
<212> DNA
<213> artificial synthesis
<400> 78
gttcagacgt gtgctcttcc gatctccctt ccctggggtg gatcttt 47
<210> 79
<211> 42
<212> DNA
<213> artificial synthesis
<400> 79
ccctacacga cgctcttccg atcttgttta tacttctctc tg 42
<210> 80
<211> 44
<212> DNA
<213> artificial synthesis
<400> 80
gttcagacgt gtgctcttcc gatctacatt tcattcaaga aaag 44
<210> 81
<211> 43
<212> DNA
<213> artificial synthesis
<400> 81
ccctacacga cgctcttccg atctatttaa taatggaatt taa 43
<210> 82
<211> 45
<212> DNA
<213> artificial synthesis
<400> 82
gttcagacgt gtgctcttcc gatctttggg tggaaaaagg taaaa 45
<210> 83
<211> 45
<212> DNA
<213> artificial synthesis
<400> 83
ccctacacga cgctcttccg atctccaacc atggagagca gggcc 45
<210> 84
<211> 54
<212> DNA
<213> artificial synthesis
<400> 84
gttcagacgt gtgctcttcc gatctaccca gccagagagg gtcaaggaaa ccag 54
<210> 85
<211> 44
<212> DNA
<213> artificial synthesis
<400> 85
ccctacacga cgctcttccg atctttagtc ataatgtttt tttt 44
<210> 86
<211> 48
<212> DNA
<213> artificial synthesis
<400> 86
gttcagacgt gtgctcttcc gatctccaca ttgaaaagta aaaagtta 48
<210> 87
<211> 54
<212> DNA
<213> artificial synthesis
<400> 87
ccctacacga cgctcttccg atctatacca tataaagaag cagaaaacat attt 54
<210> 88
<211> 49
<212> DNA
<213> artificial synthesis
<400> 88
gttcagacgt gtgctcttcc gatctgtctt gagaaataaa tataaacta 49
<210> 89
<211> 48
<212> DNA
<213> artificial synthesis
<400> 89
ccctacacga cgctcttccg atcttagtgt acactttcca taatcttg 48
<210> 90
<211> 50
<212> DNA
<213> artificial synthesis
<400> 90
gttcagacgt gtgctcttcc gatcttgaga ggcaaacaga aaagtgagag 50
<210> 91
<211> 42
<212> DNA
<213> artificial synthesis
<400> 91
ccctacacga cgctcttccg atctggaaat gcaaatcaaa at 42
<210> 92
<211> 54
<212> DNA
<213> artificial synthesis
<400> 92
gttcagacgt gtgctcttcc gatctgcagt gtacaaacat tcccttttct ttgc 54
<210> 93
<211> 55
<212> DNA
<213> artificial synthesis
<400> 93
ccctacacga cgctcttccg atcttaatac aaaaattatg gtacattcat acaat 55
<210> 94
<211> 42
<212> DNA
<213> artificial synthesis
<400> 94
gttcagacgt gtgctcttcc gatctctcat aattttgcaa ag 42
<210> 95
<211> 53
<212> DNA
<213> artificial synthesis
<400> 95
ccctacacga cgctcttccg atctatgttt tagttctatg cactataatt aat 53
<210> 96
<211> 46
<212> DNA
<213> artificial synthesis
<400> 96
gttcagacgt gtgctcttcc gatctccatc gcgcgttaag ctggga 46
<210> 97
<211> 43
<212> DNA
<213> artificial synthesis
<400> 97
ccctacacga cgctcttccg atcttgagga gttttgtctg cgg 43
<210> 98
<211> 46
<212> DNA
<213> artificial synthesis
<400> 98
gttcagacgt gtgctcttcc gatctggagt ctccaaaata tgcttt 46
<210> 99
<211> 49
<212> DNA
<213> artificial synthesis
<400> 99
ccctacacga cgctcttccg atctaaacaa cagacataaa aactcaaat 49
<210> 100
<211> 56
<212> DNA
<213> artificial synthesis
<400> 100
gttcagacgt gtgctcttcc gatctatatt gatttatact aagaaatttg ttctac 56
<210> 101
<211> 55
<212> DNA
<213> artificial synthesis
<400> 101
ccctacacga cgctcttccg atcttactca gaatatagtg tttaagttat agatt 55
<210> 102
<211> 51
<212> DNA
<213> artificial synthesis
<400> 102
gttcagacgt gtgctcttcc gatcttactt ccgtggtcct caacaatttt t 51
<210> 103
<211> 54
<212> DNA
<213> artificial synthesis
<400> 103
ccctacacga cgctcttccg atctcaggct atttaatact ataagatgtt gtgt 54
<210> 104
<211> 44
<212> DNA
<213> artificial synthesis
<400> 104
gttcagacgt gtgctcttcc gatcttaaat ttctatatat aaaa 44
<210> 105
<211> 40
<212> DNA
<213> artificial synthesis
<400> 105
ccctacacga cgctcttccg atcttatttc tttttacatt 40
<210> 106
<211> 47
<212> DNA
<213> artificial synthesis
<400> 106
gttcagacgt gtgctcttcc gatctttctt cactgcaaag cttagtc 47
<210> 107
<211> 40
<212> DNA
<213> artificial synthesis
<400> 107
ccctacacga cgctcttccg atctagaccc aacatgtgga 40
<210> 108
<211> 53
<212> DNA
<213> artificial synthesis
<400> 108
gttcagacgt gtgctcttcc gatcttctct ttccagttct gtccctttct aac 53
<210> 109
<211> 45
<212> DNA
<213> artificial synthesis
<400> 109
ccctacacga cgctcttccg atctcagctt ctgactgctg ggctc 45
<210> 110
<211> 43
<212> DNA
<213> artificial synthesis
<400> 110
gttcagacgt gtgctcttcc gatctatttg aaaccacatt tgt 43
<210> 111
<211> 43
<212> DNA
<213> artificial synthesis
<400> 111
ccctacacga cgctcttccg atctggtatc tcctcagcct ctg 43
<210> 112
<211> 55
<212> DNA
<213> artificial synthesis
<400> 112
gttcagacgt gtgctcttcc gatctttgag tttgttattg tgttatgata actat 55
<210> 113
<211> 46
<212> DNA
<213> artificial synthesis
<400> 113
ccctacacga cgctcttccg atcttggact tctccccaga ctcctg 46
<210> 114
<211> 47
<212> DNA
<213> artificial synthesis
<400> 114
gttcagacgt gtgctcttcc gatctccagc tttgatgcag gataaaa 47
<210> 115
<211> 54
<212> DNA
<213> artificial synthesis
<400> 115
ccctacacga cgctcttccg atctccctgc accaccctgc taccatcact ctat 54
<210> 116
<211> 54
<212> DNA
<213> artificial synthesis
<400> 116
gttcagacgt gtgctcttcc gatctggtga cctgaacaga aaggtggctg cccc 54
<210> 117
<211> 47
<212> DNA
<213> artificial synthesis
<400> 117
ccctacacga cgctcttccg atctggaatt acaaggggtc ccaggaa 47
<210> 118
<211> 51
<212> DNA
<213> artificial synthesis
<400> 118
gttcagacgt gtgctcttcc gatctcctgt tccccgtggg gtcctcagca c 51
<210> 119
<211> 53
<212> DNA
<213> artificial synthesis
<400> 119
ccctacacga cgctcttccg atcttctaaa ggtgggaggt gccaggctca cca 53
<210> 120
<211> 43
<212> DNA
<213> artificial synthesis
<400> 120
gttcagacgt gtgctcttcc gatctggatt atgaagatta aat 43
<210> 121
<211> 51
<212> DNA
<213> artificial synthesis
<400> 121
ccctacacga cgctcttccg atctatgata tttggttttc tgcgttaatt t 51
<210> 122
<211> 55
<212> DNA
<213> artificial synthesis
<400> 122
gttcagacgt gtgctcttcc gatctggtgt tctgactgca ggatcttcac ccata 55
<210> 123
<211> 45
<212> DNA
<213> artificial synthesis
<400> 123
ccctacacga cgctcttccg atctttacca tgtagtaggt gtgct 45
<210> 124
<211> 50
<212> DNA
<213> artificial synthesis
<400> 124
gttcagacgt gtgctcttcc gatctcataa ctgattttaa tttagcattt 50
<210> 125
<211> 41
<212> DNA
<213> artificial synthesis
<400> 125
ccctacacga cgctcttccg atcttctccc tcaaccagat a 41
<210> 126
<211> 43
<212> DNA
<213> artificial synthesis
<400> 126
gttcagacgt gtgctcttcc gatctgtcgt attcagtgtg gct 43
<210> 127
<211> 50
<212> DNA
<213> artificial synthesis
<400> 127
ccctacacga cgctcttccg atcttgagta tagttttatt ggaacacaag 50
<210> 128
<211> 45
<212> DNA
<213> artificial synthesis
<400> 128
gttcagacgt gtgctcttcc gatctagtac atagctttta gagtc 45
<210> 129
<211> 42
<212> DNA
<213> artificial synthesis
<400> 129
ccctacacga cgctcttccg atctaaaaac tggtctgttc gg 42
<210> 130
<211> 41
<212> DNA
<213> artificial synthesis
<400> 130
gttcagacgt gtgctcttcc gatctactaa gaatctgcag t 41
<210> 131
<211> 40
<212> DNA
<213> artificial synthesis
<400> 131
ccctacacga cgctcttccg atcttacaga aagtagattc 40
<210> 132
<211> 43
<212> DNA
<213> artificial synthesis
<400> 132
gttcagacgt gtgctcttcc gatctgtgtt tctagtatat tca 43
<210> 133
<211> 51
<212> DNA
<213> artificial synthesis
<400> 133
ccctacacga cgctcttccg atctgtgttt ggtgatcttg ctgtttaaaa t 51
<210> 134
<211> 44
<212> DNA
<213> artificial synthesis
<400> 134
gttcagacgt gtgctcttcc gatcttaaca cctatatttt ctcc 44
<210> 135
<211> 54
<212> DNA
<213> artificial synthesis
<400> 135
ccctacacga cgctcttccg atcttttacg gttattccta atttgcccat ttca 54
<210> 136
<211> 53
<212> DNA
<213> artificial synthesis
<400> 136
gttcagacgt gtgctcttcc gatcttgttt accacatcct ctgagttatg cag 53
<210> 137
<211> 42
<212> DNA
<213> artificial synthesis
<400> 137
ccctacacga cgctcttccg atctctctgt tctcctctat tt 42
<210> 138
<211> 54
<212> DNA
<213> artificial synthesis
<400> 138
gttcagacgt gtgctcttcc gatctaaacc ttgttcatgt agattcctct ggta 54
<210> 139
<211> 52
<212> DNA
<213> artificial synthesis
<400> 139
ccctacacga cgctcttccg atctgaagtt ccagacatgt atgttgctat ga 52
<210> 140
<211> 43
<212> DNA
<213> artificial synthesis
<400> 140
gttcagacgt gtgctcttcc gatctatttc tgtagatttt act 43
<210> 141
<211> 53
<212> DNA
<213> artificial synthesis
<400> 141
ccctacacga cgctcttccg atctatgttc aagtaattca gcaatctgcc tga 53
<210> 142
<211> 49
<212> DNA
<213> artificial synthesis
<400> 142
gttcagacgt gtgctcttcc gatctgcatt gtggttttga tttgcattt 49
<210> 143
<211> 47
<212> DNA
<213> artificial synthesis
<400> 143
ccctacacga cgctcttccg atctaattta tccatgtaac caaacac 47
<210> 144
<211> 55
<212> DNA
<213> artificial synthesis
<400> 144
gttcagacgt gtgctcttcc gatctgctgc ttatatatca gtatatatgg tacat 55
<210> 145
<211> 48
<212> DNA
<213> artificial synthesis
<400> 145
ccctacacga cgctcttccg atctaacata atgagctcca gttctatc 48
<210> 146
<211> 48
<212> DNA
<213> artificial synthesis
<400> 146
gttcagacgt gtgctcttcc gatctgcact ctttacaata gctaactt 48
<210> 147
<211> 47
<212> DNA
<213> artificial synthesis
<400> 147
ccctacacga cgctcttccg atctaatact ttgaactttt ctgatgc 47
<210> 148
<211> 48
<212> DNA
<213> artificial synthesis
<400> 148
gttcagacgt gtgctcttcc gatctatctc ttcttaatgg aaaagtat 48
<210> 149
<211> 46
<212> DNA
<213> artificial synthesis
<400> 149
ccctacacga cgctcttccg atctttgttt gtaaaaaatt aaattt 46
<210> 150
<211> 56
<212> DNA
<213> artificial synthesis
<400> 150
gttcagacgt gtgctcttcc gatctaccat aaaaaatttt agacaaataa tgttaa 56
<210> 151
<211> 40
<212> DNA
<213> artificial synthesis
<400> 151
ccctacacga cgctcttccg atctacagaa cgagaacttg 40
<210> 152
<211> 43
<212> DNA
<213> artificial synthesis
<400> 152
gttcagacgt gtgctcttcc gatctcgtgt ctcctagatc tgg 43
<210> 153
<211> 40
<212> DNA
<213> artificial synthesis
<400> 153
ccctacacga cgctcttccg atcttaggat aataatagaa 40
<210> 154
<211> 41
<212> DNA
<213> artificial synthesis
<400> 154
gttcagacgt gtgctcttcc gatctataat ttacttttca g 41
<210> 155
<211> 48
<212> DNA
<213> artificial synthesis
<400> 155
ccctacacga cgctcttccg atctgaggtg ctggtaaaaa tgattcat 48
<210> 156
<211> 48
<212> DNA
<213> artificial synthesis
<400> 156
gttcagacgt gtgctcttcc gatctctcct ggctcttcat atcttagc 48
<210> 157
<211> 51
<212> DNA
<213> artificial synthesis
<400> 157
ccctacacga cgctcttccg atcttgctgc caattaccat caaaaaagca t 51
<210> 158
<211> 51
<212> DNA
<213> artificial synthesis
<400> 158
gttcagacgt gtgctcttcc gatctagtac tgtgaagaca agcatttttt t 51
<210> 159
<211> 55
<212> DNA
<213> artificial synthesis
<400> 159
ccctacacga cgctcttccg atctatcagt ataaaagttg agaggttaaa aacat 55
<210> 160
<211> 42
<212> DNA
<213> artificial synthesis
<400> 160
gttcagacgt gtgctcttcc gatctaagtg tcttagaaaa tt 42
<210> 161
<211> 43
<212> DNA
<213> artificial synthesis
<400> 161
ccctacacga cgctcttccg atcttacata tatttctcca ctt 43
<210> 162
<211> 47
<212> DNA
<213> artificial synthesis
<400> 162
gttcagacgt gtgctcttcc gatctgattt ccagtatctg ttttaat 47
<210> 163
<211> 45
<212> DNA
<213> artificial synthesis
<400> 163
ccctacacga cgctcttccg atctatttcc atcttcagga ttcag 45
<210> 164
<211> 45
<212> DNA
<213> artificial synthesis
<400> 164
gttcagacgt gtgctcttcc gatcttggtt taatagttaa ctttt 45
<210> 165
<211> 48
<212> DNA
<213> artificial synthesis
<400> 165
ccctacacga cgctcttccg atctgactta ttacaaagct acagtaat 48
<210> 166
<211> 42
<212> DNA
<213> artificial synthesis
<400> 166
gttcagacgt gtgctcttcc gatctttttt gcatatgaat at 42
<210> 167
<211> 40
<212> DNA
<213> artificial synthesis
<400> 167
ccctacacga cgctcttccg atctgccttt aagtttacaa 40
<210> 168
<211> 43
<212> DNA
<213> artificial synthesis
<400> 168
gttcagacgt gtgctcttcc gatctaatat cagataagag aag 43
<210> 169
<211> 51
<212> DNA
<213> artificial synthesis
<400> 169
ccctacacga cgctcttccg atctgatgta tgcacatata gctatgaatt t 51
<210> 170
<211> 50
<212> DNA
<213> artificial synthesis
<400> 170
gttcagacgt gtgctcttcc gatctcaaac atgtggaaat taaacaacat 50
<210> 171
<211> 49
<212> DNA
<213> artificial synthesis
<400> 171
ccctacacga cgctcttccg atctaaaggt gcatctaaga atagtatca 49
<210> 172
<211> 43
<212> DNA
<213> artificial synthesis
<400> 172
gttcagacgt gtgctcttcc gatctggcag ggcataaata aag 43
<210> 173
<211> 43
<212> DNA
<213> artificial synthesis
<400> 173
ccctacacga cgctcttccg atctaaaaca agcttgaaat tct 43
<210> 174
<211> 55
<212> DNA
<213> artificial synthesis
<400> 174
gttcagacgt gtgctcttcc gatctagcaa atacttaaaa tttcctacaa gattt 55
<210> 175
<211> 54
<212> DNA
<213> artificial synthesis
<400> 175
ccctacacga cgctcttccg atcttccatt ttgagtgctg agaggtgaga ttag 54
<210> 176
<211> 49
<212> DNA
<213> artificial synthesis
<400> 176
gttcagacgt gtgctcttcc gatctaaaaa taacaatcac caagtctgg 49
<210> 177
<211> 49
<212> DNA
<213> artificial synthesis
<400> 177
ccctacacga cgctcttccg atctttaaat gcactgattg aacctattt 49
<210> 178
<211> 50
<212> DNA
<213> artificial synthesis
<400> 178
gttcagacgt gtgctcttcc gatcttaata agctctgctt gtatttgtat 50
<210> 179
<211> 55
<212> DNA
<213> artificial synthesis
<400> 179
ccctacacga cgctcttccg atctcatgcc ctgtcccagg cctcgctctt gactc 55
<210> 180
<211> 45
<212> DNA
<213> artificial synthesis
<400> 180
gttcagacgt gtgctcttcc gatcttaacc tttaagaaac aatta 45
<210> 181
<211> 45
<212> DNA
<213> artificial synthesis
<400> 181
ccctacacga cgctcttccg atctctggca ccaagccgag caccc 45
<210> 182
<211> 54
<212> DNA
<213> artificial synthesis
<400> 182
gttcagacgt gtgctcttcc gatctgcgcc cgcatcatcc cctctgcaaa gcgg 54
<210> 183
<211> 44
<212> DNA
<213> artificial synthesis
<400> 183
ccctacacga cgctcttccg atctgtcttt atgactgtgg taag 44
<210> 184
<211> 52
<212> DNA
<213> artificial synthesis
<400> 184
gttcagacgt gtgctcttcc gatcttatag caaacaatac actagctctc aa 52
<210> 185
<211> 55
<212> DNA
<213> artificial synthesis
<400> 185
ccctacacga cgctcttccg atctatataa atgtgtgctt tattttcctc ctaaa 55
<210> 186
<211> 47
<212> DNA
<213> artificial synthesis
<400> 186
gttcagacgt gtgctcttcc gatctaggta gaactagccc cagacag 47
<210> 187
<211> 52
<212> DNA
<213> artificial synthesis
<400> 187
ccctacacga cgctcttccg atcttagtga tcttcatgca gaaatctaaa gg 52
<210> 188
<211> 49
<212> DNA
<213> artificial synthesis
<400> 188
gttcagacgt gtgctcttcc gatctatccg acctcttctc tgattccgt 49
<210> 189
<211> 42
<212> DNA
<213> artificial synthesis
<400> 189
ccctacacga cgctcttccg atctaagcca cgtgttagtt aa 42
<210> 190
<211> 47
<212> DNA
<213> artificial synthesis
<400> 190
gttcagacgt gtgctcttcc gatcttggga ttacaggcgt gagccac 47
<210> 191
<211> 47
<212> DNA
<213> artificial synthesis
<400> 191
ccctacacga cgctcttccg atctattcat taaaagaaaa acaacaa 47
<210> 192
<211> 51
<212> DNA
<213> artificial synthesis
<400> 192
gttcagacgt gtgctcttcc gatcttcttc ccgaattaca ttgtgtttat t 51
<210> 193
<211> 47
<212> DNA
<213> artificial synthesis
<400> 193
ccctacacga cgctcttccg atctaactgc aatagtctcc aaattta 47
<210> 194
<211> 42
<212> DNA
<213> artificial synthesis
<400> 194
gttcagacgt gtgctcttcc gatctacatt ttgtcttgtt ag 42
<210> 195
<211> 54
<212> DNA
<213> artificial synthesis
<400> 195
ccctacacga cgctcttccg atctcatgtg ggaattcaag atgagatttg gatg 54
<210> 196
<211> 46
<212> DNA
<213> artificial synthesis
<400> 196
gttcagacgt gtgctcttcc gatctgccca gtgtgccctg gaaagc 46
<210> 197
<211> 41
<212> DNA
<213> artificial synthesis
<400> 197
ccctacacga cgctcttccg atcttttctg cattgtttac t 41
<210> 198
<211> 56
<212> DNA
<213> artificial synthesis
<400> 198
gttcagacgt gtgctcttcc gatctgttga tgccaggacc tctatctgtt gggctg 56
<210> 199
<211> 55
<212> DNA
<213> artificial synthesis
<400> 199
ccctacacga cgctcttccg atcttctatg gctattgttg acttctccgt tctct 55
<210> 200
<211> 52
<212> DNA
<213> artificial synthesis
<400> 200
gttcagacgt gtgctcttcc gatctgcctt caggaattgg cagttttgct ga 52
<210> 201
<211> 54
<212> DNA
<213> artificial synthesis
<400> 201
ccctacacga cgctcttccg atctgctact ttaaagctac tttgcaactt cttc 54
<210> 202
<211> 51
<212> DNA
<213> artificial synthesis
<400> 202
gttcagacgt gtgctcttcc gatcttttcg aaaagggagg agcagaaaat g 51
<210> 203
<211> 40
<212> DNA
<213> artificial synthesis
<400> 203
ccctacacga cgctcttccg atctgcagta ataagctcat 40
<210> 204
<211> 49
<212> DNA
<213> artificial synthesis
<400> 204
gttcagacgt gtgctcttcc gatctaatgg agcaaaggga gtaacactt 49
<210> 205
<211> 52
<212> DNA
<213> artificial synthesis
<400> 205
ccctacacga cgctcttccg atctaaaagg aagatacaga aacataaaag aa 52
<210> 206
<211> 56
<212> DNA
<213> artificial synthesis
<400> 206
gttcagacgt gtgctcttcc gatctgataa tacggtgaag ctgctaaatc aactaa 56
<210> 207
<211> 43
<212> DNA
<213> artificial synthesis
<400> 207
ccctacacga cgctcttccg atctaagcaa actatagtaa ccc 43
<210> 208
<211> 47
<212> DNA
<213> artificial synthesis
<400> 208
gttcagacgt gtgctcttcc gatcttgacc tcctgattct ccaactg 47
<210> 209
<211> 40
<212> DNA
<213> artificial synthesis
<400> 209
ccctacacga cgctcttccg atctaaaatt attaacatat 40
<210> 210
<211> 49
<212> DNA
<213> artificial synthesis
<400> 210
gttcagacgt gtgctcttcc gatctaataa atataagaat tatcatgta 49
<210> 211
<211> 44
<212> DNA
<213> artificial synthesis
<400> 211
ccctacacga cgctcttccg atctgtaaaa ggaagagtgt taag 44
<210> 212
<211> 46
<212> DNA
<213> artificial synthesis
<400> 212
gttcagacgt gtgctcttcc gatctcatga aaccaaaagg cttttg 46
<210> 213
<211> 52
<212> DNA
<213> artificial synthesis
<400> 213
ccctacacga cgctcttccg atctcattcg cttagatttt tttttcactt tc 52
<210> 214
<211> 44
<212> DNA
<213> artificial synthesis
<400> 214
gttcagacgt gtgctcttcc gatcttgttc taagttgtat tcaa 44
<210> 215
<211> 50
<212> DNA
<213> artificial synthesis
<400> 215
ccctacacga cgctcttccg atctttcatt cctttcttca ttcctttgtt 50
<210> 216
<211> 42
<212> DNA
<213> artificial synthesis
<400> 216
gttcagacgt gtgctcttcc gatctcacaa aatggttagt aa 42
<210> 217
<211> 49
<212> DNA
<213> artificial synthesis
<400> 217
ccctacacga cgctcttccg atctttctag tgcaggtatt gttactaac 49
<210> 218
<211> 54
<212> DNA
<213> artificial synthesis
<400> 218
gttcagacgt gtgctcttcc gatctataat attaaataaa tatataaatg gaag 54
<210> 219
<211> 48
<212> DNA
<213> artificial synthesis
<400> 219
ccctacacga cgctcttccg atctaatatc cattttcctc catttttt 48
<210> 220
<211> 53
<212> DNA
<213> artificial synthesis
<400> 220
gttcagacgt gtgctcttcc gatctatatt ttcaagttat aacaaatgtg aat 53
<210> 221
<211> 50
<212> DNA
<213> artificial synthesis
<400> 221
ccctacacga cgctcttccg atcttctgct gttcagtgtg tgacactttt 50
<210> 222
<211> 53
<212> DNA
<213> artificial synthesis
<400> 222
gttcagacgt gtgctcttcc gatctcaagg cttcacaata ttttgttaag tag 53
<210> 223
<211> 45
<212> DNA
<213> artificial synthesis
<400> 223
ccctacacga cgctcttccg atcttgataa ttttcacact ttttt 45
<210> 224
<211> 45
<212> DNA
<213> artificial synthesis
<400> 224
gttcagacgt gtgctcttcc gatcttccta ttttttttta aacta 45
<210> 225
<211> 47
<212> DNA
<213> artificial synthesis
<400> 225
ccctacacga cgctcttccg atcttgctac tgcccatagt atgtatg 47
<210> 226
<211> 43
<212> DNA
<213> artificial synthesis
<400> 226
gttcagacgt gtgctcttcc gatctgcctg ggacatttta aca 43
<210> 227
<211> 43
<212> DNA
<213> artificial synthesis
<400> 227
ccctacacga cgctcttccg atctgctgtc tcagaaaaaa tat 43
<210> 228
<211> 41
<212> DNA
<213> artificial synthesis
<400> 228
gttcagacgt gtgctcttcc gatctgacat tgtgtgaata g 41
<210> 229
<211> 47
<212> DNA
<213> artificial synthesis
<400> 229
ccctacacga cgctcttccg atctttctta aaaaatttat cggggtt 47
<210> 230
<211> 56
<212> DNA
<213> artificial synthesis
<400> 230
gttcagacgt gtgctcttcc gatctggtca aaaaaggagg tagaatagaa taaaaa 56
<210> 231
<211> 51
<212> DNA
<213> artificial synthesis
<400> 231
ccctacacga cgctcttccg atctctctcc tgctggacat gaccaagaag a 51
<210> 232
<211> 52
<212> DNA
<213> artificial synthesis
<400> 232
gttcagacgt gtgctcttcc gatctcatat tatcaggtgc ctgggttctt gt 52
<210> 233
<211> 48
<212> DNA
<213> artificial synthesis
<400> 233
ccctacacga cgctcttccg atctttaaga tagaggagag aagatcag 48
<210> 234
<211> 48
<212> DNA
<213> artificial synthesis
<400> 234
gttcagacgt gtgctcttcc gatctaaaag aaggaagaaa ggaagtag 48
<210> 235
<211> 55
<212> DNA
<213> artificial synthesis
<400> 235
ccctacacga cgctcttccg atcttgatag aataaaataa taaatttgca tagca 55
<210> 236
<211> 42
<212> DNA
<213> artificial synthesis
<400> 236
gttcagacgt gtgctcttcc gatcttttta taccacactt ta 42
<210> 237
<211> 44
<212> DNA
<213> artificial synthesis
<400> 237
ccctacacga cgctcttccg atctttgatc atgtcaaggg caag 44
<210> 238
<211> 50
<212> DNA
<213> artificial synthesis
<400> 238
gttcagacgt gtgctcttcc gatctgccac acttttctct gacactcatg 50
<210> 239
<211> 49
<212> DNA
<213> artificial synthesis
<400> 239
ccctacacga cgctcttccg atctcacccc agcctggtgg ctgaaagca 49
<210> 240
<211> 55
<212> DNA
<213> artificial synthesis
<400> 240
gttcagacgt gtgctcttcc gatctttcat cttcaccccc aaggattgaa tgatt 55
<210> 241
<211> 54
<212> DNA
<213> artificial synthesis
<400> 241
ccctacacga cgctcttccg atctgtggtg tctgcagaga cccggagaat tggc 54
<210> 242
<211> 52
<212> DNA
<213> artificial synthesis
<400> 242
gttcagacgt gtgctcttcc gatctcataa ggactagaat tattgcaaaa gc 52
<210> 243
<211> 55
<212> DNA
<213> artificial synthesis
<400> 243
ccctacacga cgctcttccg atctgtcaat taaccttttt cttttgctct tattt 55
<210> 244
<211> 41
<212> DNA
<213> artificial synthesis
<400> 244
gttcagacgt gtgctcttcc gatcttttaa ttctgagtaa t 41
<210> 245
<211> 51
<212> DNA
<213> artificial synthesis
<400> 245
ccctacacga cgctcttccg atctaaaggg tttcaagaga ttagtagagt a 51
<210> 246
<211> 49
<212> DNA
<213> artificial synthesis
<400> 246
gttcagacgt gtgctcttcc gatctatttt atattatgcc taaataatt 49
<210> 247
<211> 43
<212> DNA
<213> artificial synthesis
<400> 247
ccctacacga cgctcttccg atctattaac agtcactaaa aag 43
<210> 248
<211> 49
<212> DNA
<213> artificial synthesis
<400> 248
gttcagacgt gtgctcttcc gatcttaatt atcaaactct tagtattat 49
<210> 249
<211> 45
<212> DNA
<213> artificial synthesis
<400> 249
ccctacacga cgctcttccg atctccaatg gaacaaaata gagaa 45
<210> 250
<211> 56
<212> DNA
<213> artificial synthesis
<400> 250
gttcagacgt gtgctcttcc gatctgggtc cagtttcatt cttctgcata tggcta 56
<210> 251
<211> 53
<212> DNA
<213> artificial synthesis
<400> 251
ccctacacga cgctcttccg atctaggtaa tatcacatta aattgtttct ctt 53
<210> 252
<211> 56
<212> DNA
<213> artificial synthesis
<400> 252
gttcagacgt gtgctcttcc gatctagatg atttcatgtg aataaatctg caaaat 56
<210> 253
<211> 40
<212> DNA
<213> artificial synthesis
<400> 253
ccctacacga cgctcttccg atctgcgtta gctctcctta 40
<210> 254
<211> 46
<212> DNA
<213> artificial synthesis
<400> 254
gttcagacgt gtgctcttcc gatctatgcc attcatgact ctggca 46
<210> 255
<211> 46
<212> DNA
<213> artificial synthesis
<400> 255
ccctacacga cgctcttccg atctctcaaa gcagcacatt cgcaag 46
<210> 256
<211> 55
<212> DNA
<213> artificial synthesis
<400> 256
gttcagacgt gtgctcttcc gatctatgag cattaaaatt aaagatgaga tttgg 55
<210> 257
<211> 52
<212> DNA
<213> artificial synthesis
<400> 257
ccctacacga cgctcttccg atctcctcag tgagtgactg gtgctttttt ct 52
<210> 258
<211> 54
<212> DNA
<213> artificial synthesis
<400> 258
gttcagacgt gtgctcttcc gatctcatat agcatttcag tcattacctt tcat 54
<210> 259
<211> 54
<212> DNA
<213> artificial synthesis
<400> 259
ccctacacga cgctcttccg atctaaacag tgtcaccaag aatatgaaaa cctc 54
<210> 260
<211> 52
<212> DNA
<213> artificial synthesis
<400> 260
gttcagacgt gtgctcttcc gatctaggaa acccagctga cttttaaaag ta 52
<210> 261
<211> 46
<212> DNA
<213> artificial synthesis
<400> 261
ccctacacga cgctcttccg atctgcttct attcttctat accatt 46
<210> 262
<211> 54
<212> DNA
<213> artificial synthesis
<400> 262
gttcagacgt gtgctcttcc gatctccaac catattcaat cattcattca ttat 54
<210> 263
<211> 48
<212> DNA
<213> artificial synthesis
<400> 263
ccctacacga cgctcttccg atctgttggg tggtggctct ctgtggac 48
<210> 264
<211> 43
<212> DNA
<213> artificial synthesis
<400> 264
gttcagacgt gtgctcttcc gatcttaaga aacaggcttg ggg 43
<210> 265
<211> 54
<212> DNA
<213> artificial synthesis
<400> 265
ccctacacga cgctcttccg atctgctgag cttacaaaca ttttgacagt atag 54
<210> 266
<211> 55
<212> DNA
<213> artificial synthesis
<400> 266
gttcagacgt gtgctcttcc gatctacagg taacatgctc taagtcaaat agatc 55
<210> 267
<211> 53
<212> DNA
<213> artificial synthesis
<400> 267
ccctacacga cgctcttccg atctaacata tttttccact gagtacacaa aat 53
<210> 268
<211> 44
<212> DNA
<213> artificial synthesis
<400> 268
gttcagacgt gtgctcttcc gatctgccag gttactgttc tccc 44
<210> 269
<211> 52
<212> DNA
<213> artificial synthesis
<400> 269
ccctacacga cgctcttccg atctcccaaa ccaactcttc tctgagctcc ta 52
<210> 270
<211> 45
<212> DNA
<213> artificial synthesis
<400> 270
gttcagacgt gtgctcttcc gatctctttt gtcccactgc aatgc 45
<210> 271
<211> 40
<212> DNA
<213> artificial synthesis
<400> 271
ccctacacga cgctcttccg atctccctct tcaggaaaga 40
<210> 272
<211> 47
<212> DNA
<213> artificial synthesis
<400> 272
gttcagacgt gtgctcttcc gatctacctg caggaccaat cttggga 47
<210> 273
<211> 52
<212> DNA
<213> artificial synthesis
<400> 273
ccctacacga cgctcttccg atcttgtact agcacaaacc tagatggtac ag 52
<210> 274
<211> 48
<212> DNA
<213> artificial synthesis
<400> 274
gttcagacgt gtgctcttcc gatcttttat tacctctcct atttttaa 48
<210> 275
<211> 44
<212> DNA
<213> artificial synthesis
<400> 275
ccctacacga cgctcttccg atctagttaa tgggttaaaa cagt 44
<210> 276
<211> 44
<212> DNA
<213> artificial synthesis
<400> 276
gttcagacgt gtgctcttcc gatctaataa ctcagagaac atta 44
<210> 277
<211> 46
<212> DNA
<213> artificial synthesis
<400> 277
ccctacacga cgctcttccg atctgttaat atccagaata tataag 46
<210> 278
<211> 49
<212> DNA
<213> artificial synthesis
<400> 278
gttcagacgt gtgctcttcc gatcttagca atgttgagca tttttacct 49
<210> 279
<211> 54
<212> DNA
<213> artificial synthesis
<400> 279
ccctacacga cgctcttccg atctgttttg tggatttctt tttttttttt tttt 54
<210> 280
<211> 55
<212> DNA
<213> artificial synthesis
<400> 280
gttcagacgt gtgctcttcc gatcttctca gctactaggg aagctgaggt gggag 55
<210> 281
<211> 48
<212> DNA
<213> artificial synthesis
<400> 281
ccctacacga cgctcttccg atctgtatct ccagatatgt tccatatg 48
<210> 282
<211> 55
<212> DNA
<213> artificial synthesis
<400> 282
gttcagacgt gtgctcttcc gatctatttt ttctttcctg acctctatca tcctg 55
<210> 283
<211> 52
<212> DNA
<213> artificial synthesis
<400> 283
ccctacacga cgctcttccg atctgatatt cacacgcaaa ataataaact ta 52
<210> 284
<211> 41
<212> DNA
<213> artificial synthesis
<400> 284
gttcagacgt gtgctcttcc gatctaaatc cattatcaaa t 41
<210> 285
<211> 54
<212> DNA
<213> artificial synthesis
<400> 285
ccctacacga cgctcttccg atctccaact cattttcttc ctacactaaa gatt 54
<210> 286
<211> 41
<212> DNA
<213> artificial synthesis
<400> 286
gttcagacgt gtgctcttcc gatctctact tgagttactt a 41
<210> 287
<211> 42
<212> DNA
<213> artificial synthesis
<400> 287
ccctacacga cgctcttccg atctatctcg atgcatttcc tc 42
<210> 288
<211> 42
<212> DNA
<213> artificial synthesis
<400> 288
gttcagacgt gtgctcttcc gatctcattg ctgagaatat ta 42
<210> 289
<211> 47
<212> DNA
<213> artificial synthesis
<400> 289
ccctacacga cgctcttccg atcttgtaaa gtacctagca tagcacc 47
<210> 290
<211> 45
<212> DNA
<213> artificial synthesis
<400> 290
gttcagacgt gtgctcttcc gatctataca gccaaggatt gcttt 45
<210> 291
<211> 54
<212> DNA
<213> artificial synthesis
<400> 291
ccctacacga cgctcttccg atctagtgct gcaaaagcag aaaattcctt aatt 54
<210> 292
<211> 53
<212> DNA
<213> artificial synthesis
<400> 292
gttcagacgt gtgctcttcc gatcttggcc tgcattttca gttgtataat ctt 53
<210> 293
<211> 40
<212> DNA
<213> artificial synthesis
<400> 293
ccctacacga cgctcttccg atctctgcac caaaataaca 40
<210> 294
<211> 53
<212> DNA
<213> artificial synthesis
<400> 294
gttcagacgt gtgctcttcc gatctgggaa gggtgaccct ccagagggcc tct 53
<210> 295
<211> 41
<212> DNA
<213> artificial synthesis
<400> 295
ccctacacga cgctcttccg atctatggaa aatggatgaa g 41
<210> 296
<211> 51
<212> DNA
<213> artificial synthesis
<400> 296
gttcagacgt gtgctcttcc gatctcccct gtgcataaag acagcttcat t 51
<210> 297
<211> 53
<212> DNA
<213> artificial synthesis
<400> 297
ccctacacga cgctcttccg atctcaaaga tgtcaaccag ccaaaaagca gat 53
<210> 298
<211> 45
<212> DNA
<213> artificial synthesis
<400> 298
gttcagacgt gtgctcttcc gatctgcttg caatctttct tgggg 45
<210> 299
<211> 44
<212> DNA
<213> artificial synthesis
<400> 299
ccctacacga cgctcttccg atctgaaaaa cattagaatt taag 44
<210> 300
<211> 42
<212> DNA
<213> artificial synthesis
<400> 300
gttcagacgt gtgctcttcc gatctaggaa aaaagggtaa ag 42
<210> 301
<211> 55
<212> DNA
<213> artificial synthesis
<400> 301
ccctacacga cgctcttccg atctgtcact ttaaaaaatt taaaaagaga atata 55
<210> 302
<211> 52
<212> DNA
<213> artificial synthesis
<400> 302
gttcagacgt gtgctcttcc gatctcaact cgttatcctt tgctctttta at 52
<210> 303
<211> 40
<212> DNA
<213> artificial synthesis
<400> 303
ccctacacga cgctcttccg atctatcagc tccttttctc 40
<210> 304
<211> 50
<212> DNA
<213> artificial synthesis
<400> 304
gttcagacgt gtgctcttcc gatcttagtt tgaataattc cagtgggccc 50
<210> 305
<211> 45
<212> DNA
<213> artificial synthesis
<400> 305
ccctacacga cgctcttccg atctatattc taatttttgt aaaga 45
<210> 306
<211> 52
<212> DNA
<213> artificial synthesis
<400> 306
gttcagacgt gtgctcttcc gatctctggc tttttaactt taccacagat aa 52
<210> 307
<211> 52
<212> DNA
<213> artificial synthesis
<400> 307
ccctacacga cgctcttccg atctctggga ttacagatgt gagccactgc gc 52
<210> 308
<211> 50
<212> DNA
<213> artificial synthesis
<400> 308
gttcagacgt gtgctcttcc gatctttcac aataattatt aaggactgtg 50
<210> 309
<211> 47
<212> DNA
<213> artificial synthesis
<400> 309
ccctacacga cgctcttccg atctgttggg gattagaggg aaactca 47
<210> 310
<211> 45
<212> DNA
<213> artificial synthesis
<400> 310
gttcagacgt gtgctcttcc gatctccatt tccagttcat gctat 45
<210> 311
<211> 47
<212> DNA
<213> artificial synthesis
<400> 311
ccctacacga cgctcttccg atctactaga attcatgggg cagatta 47
<210> 312
<211> 45
<212> DNA
<213> artificial synthesis
<400> 312
gttcagacgt gtgctcttcc gatcttaatt attcatttct tgaaa 45
<210> 313
<211> 46
<212> DNA
<213> artificial synthesis
<400> 313
ccctacacga cgctcttccg atctgcagct gtctttttgc ttcttg 46
<210> 314
<211> 54
<212> DNA
<213> artificial synthesis
<400> 314
gttcagacgt gtgctcttcc gatctgtatt gggattacag gtatgagcca ctgc 54
<210> 315
<211> 42
<212> DNA
<213> artificial synthesis
<400> 315
ccctacacga cgctcttccg atctcataat tatgtttttt at 42
<210> 316
<211> 54
<212> DNA
<213> artificial synthesis
<400> 316
gttcagacgt gtgctcttcc gatctaaaca ttataactta caaagtactt agaa 54
<210> 317
<211> 42
<212> DNA
<213> artificial synthesis
<400> 317
ccctacacga cgctcttccg atctttcagg agctcttgta ag 42
<210> 318
<211> 45
<212> DNA
<213> artificial synthesis
<400> 318
gttcagacgt gtgctcttcc gatcttcaaa gaaaagaatt ttcaa 45
<210> 319
<211> 53
<212> DNA
<213> artificial synthesis
<400> 319
ccctacacga cgctcttccg atcttccccg tgctgcatct cctttctgtt cgg 53
<210> 320
<211> 44
<212> DNA
<213> artificial synthesis
<400> 320
gttcagacgt gtgctcttcc gatcttattg gaagtatggt ttat 44
<210> 321
<211> 48
<212> DNA
<213> artificial synthesis
<400> 321
ccctacacga cgctcttccg atcttcaccc ccaggactga cctcttcc 48
<210> 322
<211> 52
<212> DNA
<213> artificial synthesis
<400> 322
gttcagacgt gtgctcttcc gatctgcaga aggaaccaag gctagaagca tg 52
<210> 323
<211> 51
<212> DNA
<213> artificial synthesis
<400> 323
ccctacacga cgctcttccg atctccccac atctctattt ccagttctcc a 51
<210> 324
<211> 51
<212> DNA
<213> artificial synthesis
<400> 324
gttcagacgt gtgctcttcc gatctaaact aaaactagtt ctctgaaaaa a 51
<210> 325
<211> 54
<212> DNA
<213> artificial synthesis
<400> 325
ccctacacga cgctcttccg atctcttttg catagctttt tgctatctgc tctt 54
<210> 326
<211> 55
<212> DNA
<213> artificial synthesis
<400> 326
gttcagacgt gtgctcttcc gatctctggg agactcacag tgtcattttt gcata 55
<210> 327
<211> 48
<212> DNA
<213> artificial synthesis
<400> 327
ccctacacga cgctcttccg atctgcaact gaaatttctt ccatttta 48
<210> 328
<211> 54
<212> DNA
<213> artificial synthesis
<400> 328
gttcagacgt gtgctcttcc gatcttgcag gcaatgcact tcataagaga actg 54
<210> 329
<211> 42
<212> DNA
<213> artificial synthesis
<400> 329
ccctacacga cgctcttccg atctttttta ataccttctc ct 42
<210> 330
<211> 52
<212> DNA
<213> artificial synthesis
<400> 330
gttcagacgt gtgctcttcc gatctcagaa atcaattatt ctcattaaca aa 52
<210> 331
<211> 44
<212> DNA
<213> artificial synthesis
<400> 331
ccctacacga cgctcttccg atctgcttct ttgaaaatgt caac 44
<210> 332
<211> 49
<212> DNA
<213> artificial synthesis
<400> 332
gttcagacgt gtgctcttcc gatctagaaa atatattatt aggattaat 49
<210> 333
<211> 42
<212> DNA
<213> artificial synthesis
<400> 333
ccctacacga cgctcttccg atctggagag cttacaagga aa 42
<210> 334
<211> 55
<212> DNA
<213> artificial synthesis
<400> 334
gttcagacgt gtgctcttcc gatcttgccc aaggcagagg tcctaatccc ccagt 55
<210> 335
<211> 45
<212> DNA
<213> artificial synthesis
<400> 335
ccctacacga cgctcttccg atctaggtct aaaagtccaa ggctg 45
<210> 336
<211> 43
<212> DNA
<213> artificial synthesis
<400> 336
gttcagacgt gtgctcttcc gatctcctcc cagagagaac ggc 43
<210> 337
<211> 41
<212> DNA
<213> artificial synthesis
<400> 337
ccctacacga cgctcttccg atctgtcatg atggaaatac a 41
<210> 338
<211> 48
<212> DNA
<213> artificial synthesis
<400> 338
gttcagacgt gtgctcttcc gatcttatat tcgaaacttc atctttag 48
<210> 339
<211> 52
<212> DNA
<213> artificial synthesis
<400> 339
ccctacacga cgctcttccg atctctgctc ctgttccccg agaagggctg tt 52
<210> 340
<211> 53
<212> DNA
<213> artificial synthesis
<400> 340
gttcagacgt gtgctcttcc gatctcccag gccagtgttc tttcctctaa agc 53
<210> 341
<211> 47
<212> DNA
<213> artificial synthesis
<400> 341
ccctacacga cgctcttccg atctacaggc acctcggcca gagggga 47
<210> 342
<211> 41
<212> DNA
<213> artificial synthesis
<400> 342
gttcagacgt gtgctcttcc gatctgtggt ggagatgtca t 41
<210> 343
<211> 43
<212> DNA
<213> artificial synthesis
<400> 343
ccctacacga cgctcttccg atctagaagc gacctggtat aag 43
<210> 344
<211> 52
<212> DNA
<213> artificial synthesis
<400> 344
gttcagacgt gtgctcttcc gatctaaatc catataacta tattagtctt ct 52
<210> 345
<211> 49
<212> DNA
<213> artificial synthesis
<400> 345
ccctacacga cgctcttccg atctttatag ataattatag cacagtgga 49
<210> 346
<211> 48
<212> DNA
<213> artificial synthesis
<400> 346
gttcagacgt gtgctcttcc gatctcagct cctggtcatc tctgcctg 48
<210> 347
<211> 43
<212> DNA
<213> artificial synthesis
<400> 347
ccctacacga cgctcttccg atctaatgtt ttgtagagaa aac 43
<210> 348
<211> 46
<212> DNA
<213> artificial synthesis
<400> 348
gttcagacgt gtgctcttcc gatctctttg aaagcctcag acgccc 46
<210> 349
<211> 43
<212> DNA
<213> artificial synthesis
<400> 349
ccctacacga cgctcttccg atctaacacg tgtattattc att 43
<210> 350
<211> 44
<212> DNA
<213> artificial synthesis
<400> 350
gttcagacgt gtgctcttcc gatctaatag ccccaaggca cagc 44
<210> 351
<211> 42
<212> DNA
<213> artificial synthesis
<400> 351
ccctacacga cgctcttccg atctatgtat ctatatttgt at 42
<210> 352
<211> 47
<212> DNA
<213> artificial synthesis
<400> 352
gttcagacgt gtgctcttcc gatctaaaag aaacaaaaac tattatt 47
<210> 353
<211> 49
<212> DNA
<213> artificial synthesis
<400> 353
ccctacacga cgctcttccg atctgctaca agtgagacgt aacaggtat 49
<210> 354
<211> 42
<212> DNA
<213> artificial synthesis
<400> 354
gttcagacgt gtgctcttcc gatctccctc accccagagc cc 42
<210> 355
<211> 41
<212> DNA
<213> artificial synthesis
<400> 355
ccctacacga cgctcttccg atctcataac atggacagca g 41
<210> 356
<211> 51
<212> DNA
<213> artificial synthesis
<400> 356
gttcagacgt gtgctcttcc gatctctaat tgagaatgct gcaataagat c 51
<210> 357
<211> 53
<212> DNA
<213> artificial synthesis
<400> 357
ccctacacga cgctcttccg atctaaactg ggagcgcttg ccactcagaa tga 53
<210> 358
<211> 51
<212> DNA
<213> artificial synthesis
<400> 358
gttcagacgt gtgctcttcc gatcttgcac gccagcacca agaacaagac c 51
<210> 359
<211> 54
<212> DNA
<213> artificial synthesis
<400> 359
ccctacacga cgctcttccg atctgtaatt atggaggctg gcaagtccaa aatc 54
<210> 360
<211> 54
<212> DNA
<213> artificial synthesis
<400> 360
gttcagacgt gtgctcttcc gatctaaaag ttaaaggcct gtatagcata aaag 54
<210> 361
<211> 49
<212> DNA
<213> artificial synthesis
<400> 361
ccctacacga cgctcttccg atctccactt gccagttgta gcactctgg 49
<210> 362
<211> 48
<212> DNA
<213> artificial synthesis
<400> 362
gttcagacgt gtgctcttcc gatctagaga gacaaaagac tgtggtta 48
<210> 363
<211> 53
<212> DNA
<213> artificial synthesis
<400> 363
ccctacacga cgctcttccg atcttctact gacagttcag aaccttaaac tta 53
<210> 364
<211> 50
<212> DNA
<213> artificial synthesis
<400> 364
gttcagacgt gtgctcttcc gatctattag ccattgagct gttatttaat 50
<210> 365
<211> 49
<212> DNA
<213> artificial synthesis
<400> 365
ccctacacga cgctcttccg atctgaaaat taaaccgaaa attagattc 49
<210> 366
<211> 44
<212> DNA
<213> artificial synthesis
<400> 366
gttcagacgt gtgctcttcc gatctaccat ctttatttgc ttat 44
<210> 367
<211> 50
<212> DNA
<213> artificial synthesis
<400> 367
ccctacacga cgctcttccg atctctgagt aacaagactt tctaagccaa 50
<210> 368
<211> 56
<212> DNA
<213> artificial synthesis
<400> 368
gttcagacgt gtgctcttcc gatctcaggg acagattcag accaagattt ggactc 56
<210> 369
<211> 53
<212> DNA
<213> artificial synthesis
<400> 369
ccctacacga cgctcttccg atctcccagc aggggacagg cactcaggag ggc 53
<210> 370
<211> 51
<212> DNA
<213> artificial synthesis
<400> 370
gttcagacgt gtgctcttcc gatctggccc cttcctccag gcctccaggc c 51
<210> 371
<211> 43
<212> DNA
<213> artificial synthesis
<400> 371
ccctacacga cgctcttccg atctattttt aatttcctag gtc 43
<210> 372
<211> 51
<212> DNA
<213> artificial synthesis
<400> 372
gttcagacgt gtgctcttcc gatctatata agtggacctg cacagtttaa a 51
<210> 373
<211> 55
<212> DNA
<213> artificial synthesis
<400> 373
ccctacacga cgctcttccg atctcctcgg ctgcttgtgg tcatgttctc tgcca 55
<210> 374
<211> 50
<212> DNA
<213> artificial synthesis
<400> 374
gttcagacgt gtgctcttcc gatctagaca gtcaacactg ccgtggcttt 50
<210> 375
<211> 54
<212> DNA
<213> artificial synthesis
<400> 375
ccctacacga cgctcttccg atctagagaa taataaaaac ataattagtt ttta 54
<210> 376
<211> 45
<212> DNA
<213> artificial synthesis
<400> 376
gttcagacgt gtgctcttcc gatctctgct tcacttgccc aagct 45
<210> 377
<211> 54
<212> DNA
<213> artificial synthesis
<400> 377
ccctacacga cgctcttccg atctgaaaga aggaaggaaa taatgcagag gaac 54
<210> 378
<211> 54
<212> DNA
<213> artificial synthesis
<400> 378
gttcagacgt gtgctcttcc gatctacttt gcctgggtaa ttagaaaata aaaa 54
<210> 379
<211> 43
<212> DNA
<213> artificial synthesis
<400> 379
ccctacacga cgctcttccg atctatgcat aactgtattt gta 43
<210> 380
<211> 43
<212> DNA
<213> artificial synthesis
<400> 380
gttcagacgt gtgctcttcc gatcttgaat tattcagtct ctt 43
<210> 381
<211> 52
<212> DNA
<213> artificial synthesis
<400> 381
ccctacacga cgctcttccg atctacaaat tatatcagaa tttttataag ag 52
<210> 382
<211> 54
<212> DNA
<213> artificial synthesis
<400> 382
gttcagacgt gtgctcttcc gatcttataa aacattgaga gaaactgaaa atga 54
<210> 383
<211> 42
<212> DNA
<213> artificial synthesis
<400> 383
ccctacacga cgctcttccg atctcctccc agcgctcagc tt 42
<210> 384
<211> 42
<212> DNA
<213> artificial synthesis
<400> 384
gttcagacgt gtgctcttcc gatctggtgt tgggtctgat ca 42
<210> 385
<211> 52
<212> DNA
<213> artificial synthesis
<400> 385
ccctacacga cgctcttccg atctcagtaa aaggacctgg aagaaaagat at 52
<210> 386
<211> 41
<212> DNA
<213> artificial synthesis
<400> 386
gttcagacgt gtgctcttcc gatctaatgt tgactgtaag g 41
<210> 387
<211> 46
<212> DNA
<213> artificial synthesis
<400> 387
ccctacacga cgctcttccg atctctgagt aagatcattt ggtatt 46
<210> 388
<211> 42
<212> DNA
<213> artificial synthesis
<400> 388
gttcagacgt gtgctcttcc gatctgcaat cctatcattt gc 42
<210> 389
<211> 41
<212> DNA
<213> artificial synthesis
<400> 389
ccctacacga cgctcttccg atctcagcct aggcgacaga g 41
<210> 390
<211> 49
<212> DNA
<213> artificial synthesis
<400> 390
gttcagacgt gtgctcttcc gatctaaagc actgcatagg cttaatcaa 49
<210> 391
<211> 44
<212> DNA
<213> artificial synthesis
<400> 391
ccctacacga cgctcttccg atctttcttt cccccagtga aata 44
<210> 392
<211> 50
<212> DNA
<213> artificial synthesis
<400> 392
gttcagacgt gtgctcttcc gatctgggtc ctcgctgttt cctgccatca 50
<210> 393
<211> 44
<212> DNA
<213> artificial synthesis
<400> 393
ccctacacga cgctcttccg atctcaggag aacccagagg acat 44
<210> 394
<211> 54
<212> DNA
<213> artificial synthesis
<400> 394
gttcagacgt gtgctcttcc gatctcagct ttctaataaa gacctaaaaa tgtt 54
<210> 395
<211> 44
<212> DNA
<213> artificial synthesis
<400> 395
ccctacacga cgctcttccg atcttgctca cagatgaagg gggg 44
<210> 396
<211> 42
<212> DNA
<213> artificial synthesis
<400> 396
gttcagacgt gtgctcttcc gatctaccat gtccccagct gg 42
<210> 397
<211> 49
<212> DNA
<213> artificial synthesis
<400> 397
ccctacacga cgctcttccg atctaaatgg aacaccccaa aaaggggcg 49
<210> 398
<211> 56
<212> DNA
<213> artificial synthesis
<400> 398
gttcagacgt gtgctcttcc gatctaaaaa ctttttttct ttcgtttact tttctt 56
<210> 399
<211> 50
<212> DNA
<213> artificial synthesis
<400> 399
ccctacacga cgctcttccg atctccttaa aaggctcttc aatatcattt 50
<210> 400
<211> 51
<212> DNA
<213> artificial synthesis
<400> 400
gttcagacgt gtgctcttcc gatctaatat agtatgtgga caacaactta t 51
<210> 401
<211> 43
<212> DNA
<213> artificial synthesis
<400> 401
ccctacacga cgctcttccg atctgagaag attctgttta aat 43
<210> 402
<211> 45
<212> DNA
<213> artificial synthesis
<400> 402
gttcagacgt gtgctcttcc gatctagcct atcaaggcct ccttc 45
<210> 403
<211> 51
<212> DNA
<213> artificial synthesis
<400> 403
ccctacacga cgctcttccg atcttcctcc cataatgacc tttatcaaag g 51
<210> 404
<211> 54
<212> DNA
<213> artificial synthesis
<400> 404
gttcagacgt gtgctcttcc gatctggaaa atcgaggtaa tgattgagat gcaa 54
<210> 405
<211> 40
<212> DNA
<213> artificial synthesis
<400> 405
ccctacacga cgctcttccg atctcctcca caggacactc 40
<210> 406
<211> 41
<212> DNA
<213> artificial synthesis
<400> 406
gttcagacgt gtgctcttcc gatcttcccc tccagggccg c 41
<210> 407
<211> 49
<212> DNA
<213> artificial synthesis
<400> 407
ccctacacga cgctcttccg atctaatacc accccccaaa gatatacat 49
<210> 408
<211> 49
<212> DNA
<213> artificial synthesis
<400> 408
gttcagacgt gtgctcttcc gatctaggac cctcatggtt aacactggg 49
<210> 409
<211> 43
<212> DNA
<213> artificial synthesis
<400> 409
ccctacacga cgctcttccg atctaaattg gatttccaag atc 43
<210> 410
<211> 52
<212> DNA
<213> artificial synthesis
<400> 410
gttcagacgt gtgctcttcc gatcttgggc tttctcctct ctcctcccca cc 52
<210> 411
<211> 42
<212> DNA
<213> artificial synthesis
<400> 411
ccctacacga cgctcttccg atctattaca ggcgtgagcc ac 42
<210> 412
<211> 45
<212> DNA
<213> artificial synthesis
<400> 412
gttcagacgt gtgctcttcc gatcttgtgc ctgtgtcctg gcaca 45
<210> 413
<211> 51
<212> DNA
<213> artificial synthesis
<400> 413
ccctacacga cgctcttccg atctaatagg ccaagtatga tgactagttt a 51
<210> 414
<211> 47
<212> DNA
<213> artificial synthesis
<400> 414
gttcagacgt gtgctcttcc gatctccagc ctcctttcct acaccca 47
<210> 415
<211> 43
<212> DNA
<213> artificial synthesis
<400> 415
ccctacacga cgctcttccg atctgtgaca gccgaggaga agc 43
<210> 416
<211> 53
<212> DNA
<213> artificial synthesis
<400> 416
gttcagacgt gtgctcttcc gatctaaact ccatctcaaa aataaataaa taa 53
<210> 417
<211> 46
<212> DNA
<213> artificial synthesis
<400> 417
ccctacacga cgctcttccg atctaggttt tagctattgt attatg 46
<210> 418
<211> 43
<212> DNA
<213> artificial synthesis
<400> 418
gttcagacgt gtgctcttcc gatcttatag gttcagaggt gat 43
<210> 419
<211> 45
<212> DNA
<213> artificial synthesis
<400> 419
ccctacacga cgctcttccg atcttatatg atgaacaggc accta 45
<210> 420
<211> 52
<212> DNA
<213> artificial synthesis
<400> 420
gttcagacgt gtgctcttcc gatctgccag ggtttgtttg ctttcttccc ag 52
<210> 421
<211> 43
<212> DNA
<213> artificial synthesis
<400> 421
ccctacacga cgctcttccg atctcagtca tctttaaaaa atg 43
<210> 422
<211> 52
<212> DNA
<213> artificial synthesis
<400> 422
gttcagacgt gtgctcttcc gatctaaaat aaagtattta aagcaaaaaa aa 52
<210> 423
<211> 48
<212> DNA
<213> artificial synthesis
<400> 423
ccctacacga cgctcttccg atctaaagcc cttccttcag agcaagcg 48
<210> 424
<211> 56
<212> DNA
<213> artificial synthesis
<400> 424
gttcagacgt gtgctcttcc gatctgcgct tactggctga cgttctgcgt gcgcgc 56
<210> 425
<211> 48
<212> DNA
<213> artificial synthesis
<400> 425
ccctacacga cgctcttccg atctccctgg ggatggagga aaggcaga 48
<210> 426
<211> 52
<212> DNA
<213> artificial synthesis
<400> 426
gttcagacgt gtgctcttcc gatcttgcat agggaaatga gaaaaactgc cc 52
<210> 427
<211> 48
<212> DNA
<213> artificial synthesis
<400> 427
ccctacacga cgctcttccg atctcgagac atcatgcaga aaagaagc 48
<210> 428
<211> 50
<212> DNA
<213> artificial synthesis
<400> 428
gttcagacgt gtgctcttcc gatcttgggt cagtgattca gacagagaac 50
<210> 429
<211> 44
<212> DNA
<213> artificial synthesis
<400> 429
ccctacacga cgctcttccg atctctgtct acttgagtga acca 44
<210> 430
<211> 41
<212> DNA
<213> artificial synthesis
<400> 430
gttcagacgt gtgctcttcc gatcttcagg tttctaccct a 41
<210> 431
<211> 53
<212> DNA
<213> artificial synthesis
<400> 431
ccctacacga cgctcttccg atctagtaat aataacaaca ataataaatc cag 53
<210> 432
<211> 42
<212> DNA
<213> artificial synthesis
<400> 432
gttcagacgt gtgctcttcc gatctgcatt tgcctccagc tc 42
<210> 433
<211> 48
<212> DNA
<213> artificial synthesis
<400> 433
ccctacacga cgctcttccg atcttcagag tgaattcctc ttaaattt 48
<210> 434
<211> 47
<212> DNA
<213> artificial synthesis
<400> 434
gttcagacgt gtgctcttcc gatctcagtt aactttgaaa tcaagag 47
<210> 435
<211> 49
<212> DNA
<213> artificial synthesis
<400> 435
ccctacacga cgctcttccg atcttctgaa gttacggttt tagataaga 49
<210> 436
<211> 47
<212> DNA
<213> artificial synthesis
<400> 436
gttcagacgt gtgctcttcc gatctcctgg gctcagctta tcttaac 47
<210> 437
<211> 52
<212> DNA
<213> artificial synthesis
<400> 437
ccctacacga cgctcttccg atctttgtag gggagtctat cagtgacatc ag 52
<210> 438
<211> 50
<212> DNA
<213> artificial synthesis
<400> 438
gttcagacgt gtgctcttcc gatctgagcc ccttgtgccc tgcacataca 50
<210> 439
<211> 41
<212> DNA
<213> artificial synthesis
<400> 439
ccctacacga cgctcttccg atcttggtgt tcctctttcc a 41
<210> 440
<211> 50
<212> DNA
<213> artificial synthesis
<400> 440
gttcagacgt gtgctcttcc gatctaaact cctgacctca agtgatccac 50
<210> 441
<211> 47
<212> DNA
<213> artificial synthesis
<400> 441
ccctacacga cgctcttccg atctatttac attatatctg tcttatt 47
<210> 442
<211> 48
<212> DNA
<213> artificial synthesis
<400> 442
gttcagacgt gtgctcttcc gatctttaga gggggacaaa gatctgtg 48
<210> 443
<211> 40
<212> DNA
<213> artificial synthesis
<400> 443
ccctacacga cgctcttccg atctggggta tctaaacctt 40
<210> 444
<211> 43
<212> DNA
<213> artificial synthesis
<400> 444
gttcagacgt gtgctcttcc gatctcttcc tgcaagcagc ttc 43
<210> 445
<211> 50
<212> DNA
<213> artificial synthesis
<400> 445
ccctacacga cgctcttccg atctatatgt catggttaaa aaaacacaag 50
<210> 446
<211> 51
<212> DNA
<213> artificial synthesis
<400> 446
gttcagacgt gtgctcttcc gatctgtgca ctggcctaaa aacttaatcc t 51
<210> 447
<211> 50
<212> DNA
<213> artificial synthesis
<400> 447
ccctacacga cgctcttccg atctctatct ctttcagtgc tgtcagcagc 50
<210> 448
<211> 50
<212> DNA
<213> artificial synthesis
<400> 448
gttcagacgt gtgctcttcc gatctttcat gagcgatgac attgggcaag 50
<210> 449
<211> 42
<212> DNA
<213> artificial synthesis
<400> 449
ccctacacga cgctcttccg atctccttga ttttgtagag ac 42
<210> 450
<211> 52
<212> DNA
<213> artificial synthesis
<400> 450
gttcagacgt gtgctcttcc gatcttctca gatgtcagac ctacaacatg gc 52
<210> 451
<211> 49
<212> DNA
<213> artificial synthesis
<400> 451
ccctacacga cgctcttccg atcttcaggc cagtggccat cagactttc 49
<210> 452
<211> 49
<212> DNA
<213> artificial synthesis
<400> 452
gttcagacgt gtgctcttcc gatctagagt tcccgccaca ttatcctag 49
<210> 453
<211> 49
<212> DNA
<213> artificial synthesis
<400> 453
ccctacacga cgctcttccg atcttttcag aagaggtcca gaaaggtca 49
<210> 454
<211> 56
<212> DNA
<213> artificial synthesis
<400> 454
gttcagacgt gtgctcttcc gatctgaatc attgattgtg ttttactttc tctcaa 56
<210> 455
<211> 49
<212> DNA
<213> artificial synthesis
<400> 455
ccctacacga cgctcttccg atctgaggga gagattgaaa atgtatgaa 49
<210> 456
<211> 48
<212> DNA
<213> artificial synthesis
<400> 456
gttcagacgt gtgctcttcc gatctcatgt accccagggc acagttct 48
<210> 457
<211> 41
<212> DNA
<213> artificial synthesis
<400> 457
ccctacacga cgctcttccg atctcagccg agcctgggtg g 41
<210> 458
<211> 47
<212> DNA
<213> artificial synthesis
<400> 458
gttcagacgt gtgctcttcc gatctgggga accctggagc ttttgag 47
<210> 459
<211> 40
<212> DNA
<213> artificial synthesis
<400> 459
ccctacacga cgctcttccg atcttagttc attcatttaa 40
<210> 460
<211> 56
<212> DNA
<213> artificial synthesis
<400> 460
gttcagacgt gtgctcttcc gatctgtatg gtttggataa actaagaaac caatta 56
<210> 461
<211> 55
<212> DNA
<213> artificial synthesis
<400> 461
ccctacacga cgctcttccg atctctgagc tcatactcag atctgtccct ggcct 55
<210> 462
<211> 51
<212> DNA
<213> artificial synthesis
<400> 462
gttcagacgt gtgctcttcc gatctattgg gatagaaagg acctaggtat g 51
<210> 463
<211> 44
<212> DNA
<213> artificial synthesis
<400> 463
ccctacacga cgctcttccg atctgccatg ctctcccctc tccc 44
<210> 464
<211> 46
<212> DNA
<213> artificial synthesis
<400> 464
gttcagacgt gtgctcttcc gatctaagtt tgtgctcata gagctc 46
<210> 465
<211> 41
<212> DNA
<213> artificial synthesis
<400> 465
ccctacacga cgctcttccg atctcaagac caaggggaca g 41
<210> 466
<211> 41
<212> DNA
<213> artificial synthesis
<400> 466
gttcagacgt gtgctcttcc gatctaatat tacttttctt t 41
<210> 467
<211> 42
<212> DNA
<213> artificial synthesis
<400> 467
ccctacacga cgctcttccg atctcaagga ggtaaaaata ga 42
<210> 468
<211> 56
<212> DNA
<213> artificial synthesis
<400> 468
gttcagacgt gtgctcttcc gatctggcac acagggggtg gctggcgtcc tctgaa 56
<210> 469
<211> 49
<212> DNA
<213> artificial synthesis
<400> 469
ccctacacga cgctcttccg atcttatcag ggctacaggg cagggaacc 49
<210> 470
<211> 46
<212> DNA
<213> artificial synthesis
<400> 470
gttcagacgt gtgctcttcc gatctgcagg tgtgaacaag ggttcc 46
<210> 471
<211> 43
<212> DNA
<213> artificial synthesis
<400> 471
ccctacacga cgctcttccg atcttcagac ttgtcagtca ata 43
<210> 472
<211> 47
<212> DNA
<213> artificial synthesis
<400> 472
gttcagacgt gtgctcttcc gatctcaaca attcctttct tgcagga 47
<210> 473
<211> 45
<212> DNA
<213> artificial synthesis
<400> 473
ccctacacga cgctcttccg atctggccct tggcttgccg gctgc 45
<210> 474
<211> 48
<212> DNA
<213> artificial synthesis
<400> 474
gttcagacgt gtgctcttcc gatctactat gtgcaccctc tccctttg 48
<210> 475
<211> 51
<212> DNA
<213> artificial synthesis
<400> 475
ccctacacga cgctcttccg atcttgttgg cacttgctca aaattggagg c 51
<210> 476
<211> 56
<212> DNA
<213> artificial synthesis
<400> 476
gttcagacgt gtgctcttcc gatctagtga caaatataat tgattttttt ttagct 56
<210> 477
<211> 45
<212> DNA
<213> artificial synthesis
<400> 477
ccctacacga cgctcttccg atctagtata tgtaaaacta aactt 45
<210> 478
<211> 42
<212> DNA
<213> artificial synthesis
<400> 478
gttcagacgt gtgctcttcc gatctgtaat ccagtgagat aa 42
<210> 479
<211> 44
<212> DNA
<213> artificial synthesis
<400> 479
ccctacacga cgctcttccg atctttagaa actccttttt gaaa 44
<210> 480
<211> 49
<212> DNA
<213> artificial synthesis
<400> 480
gttcagacgt gtgctcttcc gatctgtgtt gacagtgtac caggctacc 49
<210> 481
<211> 48
<212> DNA
<213> artificial synthesis
<400> 481
ccctacacga cgctcttccg atcttttttc aaagtttcat cttgtcct 48
<210> 482
<211> 41
<212> DNA
<213> artificial synthesis
<400> 482
gttcagacgt gtgctcttcc gatctataat acagtacttg g 41
<210> 483
<211> 50
<212> DNA
<213> artificial synthesis
<400> 483
ccctacacga cgctcttccg atctgacagt tttcagcatg agggcagact 50
<210> 484
<211> 45
<212> DNA
<213> artificial synthesis
<400> 484
gttcagacgt gtgctcttcc gatctgatta caggcgttag ccatc 45
<210> 485
<211> 48
<212> DNA
<213> artificial synthesis
<400> 485
ccctacacga cgctcttccg atctttaaac aaaagtaaaa agaataaa 48
<210> 486
<211> 52
<212> DNA
<213> artificial synthesis
<400> 486
gttcagacgt gtgctcttcc gatctaagtg gcatggaggg ggacaatcat ta 52
<210> 487
<211> 48
<212> DNA
<213> artificial synthesis
<400> 487
ccctacacga cgctcttccg atctactcct caccttcccc tcctgcac 48
<210> 488
<211> 50
<212> DNA
<213> artificial synthesis
<400> 488
gttcagacgt gtgctcttcc gatctagctc agaaattgct tggcgatatt 50
<210> 489
<211> 55
<212> DNA
<213> artificial synthesis
<400> 489
ccctacacga cgctcttccg atctcacaaa tttagcacct atcacattca cttag 55
<210> 490
<211> 46
<212> DNA
<213> artificial synthesis
<400> 490
gttcagacgt gtgctcttcc gatctgaaat aaagatagat aaatgt 46
<210> 491
<211> 44
<212> DNA
<213> artificial synthesis
<400> 491
ccctacacga cgctcttccg atcttgttta gagaggcagg ggca 44
<210> 492
<211> 47
<212> DNA
<213> artificial synthesis
<400> 492
gttcagacgt gtgctcttcc gatctaatgt cattaacttc tatgtag 47
<210> 493
<211> 47
<212> DNA
<213> artificial synthesis
<400> 493
ccctacacga cgctcttccg atcttattcc aaagcttggt gatacca 47
<210> 494
<211> 41
<212> DNA
<213> artificial synthesis
<400> 494
gttcagacgt gtgctcttcc gatctcttca gtctagcaat g 41
<210> 495
<211> 50
<212> DNA
<213> artificial synthesis
<400> 495
ccctacacga cgctcttccg atctcacaat tgtgtccttc caaaattcac 50
<210> 496
<211> 48
<212> DNA
<213> artificial synthesis
<400> 496
gttcagacgt gtgctcttcc gatctaaagg cactaattcc attatgag 48
<210> 497
<211> 55
<212> DNA
<213> artificial synthesis
<400> 497
ccctacacga cgctcttccg atctcaaagt ggttttctct cttaacaatt tgcct 55
<210> 498
<211> 53
<212> DNA
<213> artificial synthesis
<400> 498
gttcagacgt gtgctcttcc gatctatgag cctggaagga gtctggatca ggt 53
<210> 499
<211> 50
<212> DNA
<213> artificial synthesis
<400> 499
ccctacacga cgctcttccg atctcccaat gagttaatga aaatgaatat 50
<210> 500
<211> 44
<212> DNA
<213> artificial synthesis
<400> 500
gttcagacgt gtgctcttcc gatcttccac cctgatgact gttt 44
<210> 501
<211> 40
<212> DNA
<213> artificial synthesis
<400> 501
ccctacacga cgctcttccg atctaaatca accttctcac 40
<210> 502
<211> 45
<212> DNA
<213> artificial synthesis
<400> 502
gttcagacgt gtgctcttcc gatctttgca actttctgta tggcc 45
<210> 503
<211> 42
<212> DNA
<213> artificial synthesis
<400> 503
ccctacacga cgctcttccg atctggagaa gggctctgtt tc 42
<210> 504
<211> 43
<212> DNA
<213> artificial synthesis
<400> 504
gttcagacgt gtgctcttcc gatctcatgt acaccaccac atg 43
<210> 505
<211> 45
<212> DNA
<213> artificial synthesis
<400> 505
ccctacacga cgctcttccg atctgtcagt ggccctcaaa cctgg 45
<210> 506
<211> 47
<212> DNA
<213> artificial synthesis
<400> 506
gttcagacgt gtgctcttcc gatctaccat ccaaggcagc tggctgc 47
<210> 507
<211> 41
<212> DNA
<213> artificial synthesis
<400> 507
ccctacacga cgctcttccg atctccagca gcgggagaag c 41
<210> 508
<211> 48
<212> DNA
<213> artificial synthesis
<400> 508
gttcagacgt gtgctcttcc gatctgttcc tacggccttt tgcctctc 48
<210> 509
<211> 47
<212> DNA
<213> artificial synthesis
<400> 509
ccctacacga cgctcttccg atctcaggct tccttgtacc aaatctc 47
<210> 510
<211> 56
<212> DNA
<213> artificial synthesis
<400> 510
gttcagacgt gtgctcttcc gatctgtata cacacacaca cacacacaca cacaca 56
<210> 511
<211> 48
<212> DNA
<213> artificial synthesis
<400> 511
ccctacacga cgctcttccg atctaatacc agtaggtctt tggaaatc 48
<210> 512
<211> 52
<212> DNA
<213> artificial synthesis
<400> 512
gttcagacgt gtgctcttcc gatctggaca gactatgtat atagtaggca tt 52
<210> 513
<211> 43
<212> DNA
<213> artificial synthesis
<400> 513
ccctacacga cgctcttccg atctcaagaa gactgttatg tta 43
<210> 514
<211> 44
<212> DNA
<213> artificial synthesis
<400> 514
gttcagacgt gtgctcttcc gatctaagcc tattaattat ttcc 44
<210> 515
<211> 55
<212> DNA
<213> artificial synthesis
<400> 515
ccctacacga cgctcttccg atcttaaata aatacataaa atataatagt cctca 55
<210> 516
<211> 53
<212> DNA
<213> artificial synthesis
<400> 516
gttcagacgt gtgctcttcc gatctcacct gaccaaacaa aattaagatt ccc 53
<210> 517
<211> 44
<212> DNA
<213> artificial synthesis
<400> 517
ccctacacga cgctcttccg atctcttggg gcctttgggg aggc 44
<210> 518
<211> 44
<212> DNA
<213> artificial synthesis
<400> 518
gttcagacgt gtgctcttcc gatcttgctt tcacatccac tatc 44
<210> 519
<211> 41
<212> DNA
<213> artificial synthesis
<400> 519
ccctacacga cgctcttccg atctctttgt taattctatt t 41
<210> 520
<211> 53
<212> DNA
<213> artificial synthesis
<400> 520
gttcagacgt gtgctcttcc gatctacaag tattacccta tggtattaaa aaa 53
<210> 521
<211> 48
<212> DNA
<213> artificial synthesis
<400> 521
ccctacacga cgctcttccg atctgttatg ctgaaggagt ttgctgat 48
<210> 522
<211> 53
<212> DNA
<213> artificial synthesis
<400> 522
gttcagacgt gtgctcttcc gatctcttga agaacttaca acgtattcga aag 53
<210> 523
<211> 44
<212> DNA
<213> artificial synthesis
<400> 523
ccctacacga cgctcttccg atcttatcat cataacacca gaca 44
<210> 524
<211> 41
<212> DNA
<213> artificial synthesis
<400> 524
gttcagacgt gtgctcttcc gatctttgta gtcagataat a 41
<210> 525
<211> 48
<212> DNA
<213> artificial synthesis
<400> 525
ccctacacga cgctcttccg atctttgtat gaactttttc cttttatt 48
<210> 526
<211> 44
<212> DNA
<213> artificial synthesis
<400> 526
gttcagacgt gtgctcttcc gatctcaaca aaaaaaattc tttt 44
<210> 527
<211> 51
<212> DNA
<213> artificial synthesis
<400> 527
ccctacacga cgctcttccg atcttaattt atcattttta aatgagccct g 51
<210> 528
<211> 47
<212> DNA
<213> artificial synthesis
<400> 528
gttcagacgt gtgctcttcc gatctttatt aaaaaatcat agaactg 47
<210> 529
<211> 50
<212> DNA
<213> artificial synthesis
<400> 529
ccctacacga cgctcttccg atctcagtag aagatgatga tgtcagagag 50
<210> 530
<211> 54
<212> DNA
<213> artificial synthesis
<400> 530
gttcagacgt gtgctcttcc gatctaatcc tattttattc aagtaagagt caat 54
<210> 531
<211> 42
<212> DNA
<213> artificial synthesis
<400> 531
ccctacacga cgctcttccg atctattgaa agaaaaaaaa aa 42
<210> 532
<211> 42
<212> DNA
<213> artificial synthesis
<400> 532
gttcagacgt gtgctcttcc gatctaaaat cctttcctta tt 42
<210> 533
<211> 41
<212> DNA
<213> artificial synthesis
<400> 533
ccctacacga cgctcttccg atcttgaaat acatgtgtat a 41
<210> 534
<211> 44
<212> DNA
<213> artificial synthesis
<400> 534
gttcagacgt gtgctcttcc gatctaataa atcatctgaa attc 44
<210> 535
<211> 43
<212> DNA
<213> artificial synthesis
<400> 535
ccctacacga cgctcttccg atctaatgtc accacctgtc tta 43
<210> 536
<211> 46
<212> DNA
<213> artificial synthesis
<400> 536
gttcagacgt gtgctcttcc gatctggatg ggttggaaat ttgaaa 46
<210> 537
<211> 46
<212> DNA
<213> artificial synthesis
<400> 537
ccctacacga cgctcttccg atctcggggc aaggtctctg gggagg 46
<210> 538
<211> 52
<212> DNA
<213> artificial synthesis
<400> 538
gttcagacgt gtgctcttcc gatctgtgat aagtgactgc cgccatggga ca 52
<210> 539
<211> 54
<212> DNA
<213> artificial synthesis
<400> 539
ccctacacga cgctcttccg atcttcctgc tggccattta acactcaagc cgga 54
<210> 540
<211> 49
<212> DNA
<213> artificial synthesis
<400> 540
gttcagacgt gtgctcttcc gatctccctg gggctagagg cctagtcac 49
<210> 541
<211> 52
<212> DNA
<213> artificial synthesis
<400> 541
ccctacacga cgctcttccg atctgagtta tcagattgtc aaagtctggt gt 52
<210> 542
<211> 43
<212> DNA
<213> artificial synthesis
<400> 542
gttcagacgt gtgctcttcc gatctacctg gtgatagata aat 43
<210> 543
<211> 48
<212> DNA
<213> artificial synthesis
<400> 543
ccctacacga cgctcttccg atctgaaggc attgtccaca tacctggc 48
<210> 544
<211> 44
<212> DNA
<213> artificial synthesis
<400> 544
gttcagacgt gtgctcttcc gatctttctg ctaatgagga gtct 44
<210> 545
<211> 51
<212> DNA
<213> artificial synthesis
<400> 545
ccctacacga cgctcttccg atctacaatt tctcacataa agccttaaat a 51
<210> 546
<211> 42
<212> DNA
<213> artificial synthesis
<400> 546
gttcagacgt gtgctcttcc gatcttctgg agttaattat tc 42
<210> 547
<211> 55
<212> DNA
<213> artificial synthesis
<400> 547
ccctacacga cgctcttccg atctagcata ctatttgttt ctttccttat taagt 55
<210> 548
<211> 43
<212> DNA
<213> artificial synthesis
<400> 548
gttcagacgt gtgctcttcc gatcttggct ctgttatcag ata 43
<210> 549
<211> 48
<212> DNA
<213> artificial synthesis
<400> 549
ccctacacga cgctcttccg atcttaagat gttccacctg tgcctgct 48
<210> 550
<211> 50
<212> DNA
<213> artificial synthesis
<400> 550
gttcagacgt gtgctcttcc gatctctccc tacaagagtc tgacgtaatg 50
<210> 551
<211> 48
<212> DNA
<213> artificial synthesis
<400> 551
ccctacacga cgctcttccg atctagcaaa caaaaccaaa acaaacaa 48
<210> 552
<211> 47
<212> DNA
<213> artificial synthesis
<400> 552
gttcagacgt gtgctcttcc gatctcctca ttcagacttt cataagc 47
<210> 553
<211> 52
<212> DNA
<213> artificial synthesis
<400> 553
ccctacacga cgctcttccg atctagaaaa aaggaaaact agagaaagaa ga 52
<210> 554
<211> 46
<212> DNA
<213> artificial synthesis
<400> 554
gttcagacgt gtgctcttcc gatctcttgt cattgttcaa actatt 46
<210> 555
<211> 46
<212> DNA
<213> artificial synthesis
<400> 555
ccctacacga cgctcttccg atctgttggc tcatctcagc tgttgg 46
<210> 556
<211> 49
<212> DNA
<213> artificial synthesis
<400> 556
gttcagacgt gtgctcttcc gatctggtct tgaactcctg acctcaggt 49
<210> 557
<211> 55
<212> DNA
<213> artificial synthesis
<400> 557
ccctacacga cgctcttccg atctatgaat attgtgaatg agaaaaatat ttaat 55
<210> 558
<211> 47
<212> DNA
<213> artificial synthesis
<400> 558
gttcagacgt gtgctcttcc gatctaacaa aaaacacatt ttgatta 47
<210> 559
<211> 45
<212> DNA
<213> artificial synthesis
<400> 559
ccctacacga cgctcttccg atctcaacac ttattttaaa cagta 45
<210> 560
<211> 54
<212> DNA
<213> artificial synthesis
<400> 560
gttcagacgt gtgctcttcc gatctgggag aggcattctc aggtgttttc ctta 54
<210> 561
<211> 54
<212> DNA
<213> artificial synthesis
<400> 561
ccctacacga cgctcttccg atctagtaat cactagtaaa ggtgttccta actc 54
<210> 562
<211> 47
<212> DNA
<213> artificial synthesis
<400> 562
gttcagacgt gtgctcttcc gatctcagtg ttcttgacct caaagag 47
<210> 563
<211> 46
<212> DNA
<213> artificial synthesis
<400> 563
ccctacacga cgctcttccg atctcatctt ggagtcccaa cgtctg 46
<210> 564
<211> 55
<212> DNA
<213> artificial synthesis
<400> 564
gttcagacgt gtgctcttcc gatctgataa atggccgttc gtgttccaca gtgct 55
<210> 565
<211> 40
<212> DNA
<213> artificial synthesis
<400> 565
ccctacacga cgctcttccg atctcttcat attttataga 40
<210> 566
<211> 51
<212> DNA
<213> artificial synthesis
<400> 566
gttcagacgt gtgctcttcc gatctatggc caatcttgtt taatctttac t 51
<210> 567
<211> 55
<212> DNA
<213> artificial synthesis
<400> 567
ccctacacga cgctcttccg atctacagag tggaaagata ggcaatggag tcctg 55
<210> 568
<211> 42
<212> DNA
<213> artificial synthesis
<400> 568
gttcagacgt gtgctcttcc gatctgaggc tctgtgagaa aa 42
<210> 569
<211> 42
<212> DNA
<213> artificial synthesis
<400> 569
ccctacacga cgctcttccg atctagattt ggggggatta tt 42
<210> 570
<211> 51
<212> DNA
<213> artificial synthesis
<400> 570
gttcagacgt gtgctcttcc gatcttcaaa ctaatcatta ctgaatatta a 51
<210> 571
<211> 40
<212> DNA
<213> artificial synthesis
<400> 571
ccctacacga cgctcttccg atctagtgta ttctcagaag 40
<210> 572
<211> 45
<212> DNA
<213> artificial synthesis
<400> 572
gttcagacgt gtgctcttcc gatctgctat aaaaagactt agtta 45
<210> 573
<211> 44
<212> DNA
<213> artificial synthesis
<400> 573
ccctacacga cgctcttccg atctaccaca atatcatgta ctga 44
<210> 574
<211> 47
<212> DNA
<213> artificial synthesis
<400> 574
gttcagacgt gtgctcttcc gatcttctaa ttcatgaaat gtatctg 47
<210> 575
<211> 46
<212> DNA
<213> artificial synthesis
<400> 575
ccctacacga cgctcttccg atctgacatt tccaagctca cccaag 46
<210> 576
<211> 50
<212> DNA
<213> artificial synthesis
<400> 576
gttcagacgt gtgctcttcc gatcttaaag gcacttggct gggaatggag 50
<210> 577
<211> 40
<212> DNA
<213> artificial synthesis
<400> 577
ccctacacga cgctcttccg atctggtgtt aggaaggcca 40
<210> 578
<211> 43
<212> DNA
<213> artificial synthesis
<400> 578
gttcagacgt gtgctcttcc gatctgataa gaaaaggttg gga 43
<210> 579
<211> 48
<212> DNA
<213> artificial synthesis
<400> 579
ccctacacga cgctcttccg atctggtgaa aataagttac ttcttata 48
<210> 580
<211> 55
<212> DNA
<213> artificial synthesis
<400> 580
gttcagacgt gtgctcttcc gatctaaatt taagaatttt tcttcactta ttaag 55
<210> 581
<211> 53
<212> DNA
<213> artificial synthesis
<400> 581
ccctacacga cgctcttccg atctattaga aaagcaaaat ctgacctgaa gac 53
<210> 582
<211> 56
<212> DNA
<213> artificial synthesis
<400> 582
gttcagacgt gtgctcttcc gatctaactc ttctctcatc tcatttctaa taaata 56
<210> 583
<211> 51
<212> DNA
<213> artificial synthesis
<400> 583
ccctacacga cgctcttccg atctaaagtt gaaaaagaaa aaaagaaaaa t 51
<210> 584
<211> 48
<212> DNA
<213> artificial synthesis
<400> 584
gttcagacgt gtgctcttcc gatcttcctg tataaacaag acaaaaag 48
<210> 585
<211> 50
<212> DNA
<213> artificial synthesis
<400> 585
ccctacacga cgctcttccg atcttttaaa acttttatga actgaagtaa 50
<210> 586
<211> 52
<212> DNA
<213> artificial synthesis
<400> 586
gttcagacgt gtgctcttcc gatcttcaac tcatgtgtaa acaatgcttt tt 52
<210> 587
<211> 45
<212> DNA
<213> artificial synthesis
<400> 587
ccctacacga cgctcttccg atctggtccc tcttccccac ttaac 45
<210> 588
<211> 42
<212> DNA
<213> artificial synthesis
<400> 588
gttcagacgt gtgctcttcc gatctggtgt ggaattttcc at 42
<210> 589
<211> 54
<212> DNA
<213> artificial synthesis
<400> 589
ccctacacga cgctcttccg atctggtctt attatactca ttcctaaacc aata 54
<210> 590
<211> 55
<212> DNA
<213> artificial synthesis
<400> 590
gttcagacgt gtgctcttcc gatctctgtg ttccttgcag tatgtcaatt atttt 55
<210> 591
<211> 45
<212> DNA
<213> artificial synthesis
<400> 591
ccctacacga cgctcttccg atctagccta attcctgggc ctggg 45
<210> 592
<211> 53
<212> DNA
<213> artificial synthesis
<400> 592
gttcagacgt gtgctcttcc gatcttgagt acattcacgt agacatgacc aag 53
<210> 593
<211> 44
<212> DNA
<213> artificial synthesis
<400> 593
ccctacacga cgctcttccg atcttccagg caccaactgc tccc 44
<210> 594
<211> 43
<212> DNA
<213> artificial synthesis
<400> 594
gttcagacgt gtgctcttcc gatctagtac cccagaggat gag 43
<210> 595
<211> 49
<212> DNA
<213> artificial synthesis
<400> 595
ccctacacga cgctcttccg atctgccacc tactatacaa aatcctgaa 49
<210> 596
<211> 55
<212> DNA
<213> artificial synthesis
<400> 596
gttcagacgt gtgctcttcc gatctaaaag cctctggcta taaaaatgga tttat 55
<210> 597
<211> 42
<212> DNA
<213> artificial synthesis
<400> 597
ccctacacga cgctcttccg atctggcagg agagggaaac ac 42
<210> 598
<211> 41
<212> DNA
<213> artificial synthesis
<400> 598
gttcagacgt gtgctcttcc gatctctcca tctcttcctc c 41
<210> 599
<211> 53
<212> DNA
<213> artificial synthesis
<400> 599
ccctacacga cgctcttccg atctagtact gagacactaa gtagacattc tag 53
<210> 600
<211> 53
<212> DNA
<213> artificial synthesis
<400> 600
gttcagacgt gtgctcttcc gatctttaat ctgcacctat gatagatcta gaa 53
<210> 601
<211> 48
<212> DNA
<213> artificial synthesis
<400> 601
ccctacacga cgctcttccg atctcaacag agctttttct caaccatc 48
<210> 602
<211> 45
<212> DNA
<213> artificial synthesis
<400> 602
gttcagacgt gtgctcttcc gatctgcaaa agatttgaag ctata 45
<210> 603
<211> 52
<212> DNA
<213> artificial synthesis
<400> 603
ccctacacga cgctcttccg atcttcttta aggacccatc tgaaggagga ga 52
<210> 604
<211> 53
<212> DNA
<213> artificial synthesis
<400> 604
gttcagacgt gtgctcttcc gatctctggg atataagggg acaattgccc caa 53
<210> 605
<211> 42
<212> DNA
<213> artificial synthesis
<400> 605
ccctacacga cgctcttccg atctccaagg cctaccacta gc 42
<210> 606
<211> 46
<212> DNA
<213> artificial synthesis
<400> 606
gttcagacgt gtgctcttcc gatctacagt gggggaacaa aaagga 46
<210> 607
<211> 51
<212> DNA
<213> artificial synthesis
<400> 607
ccctacacga cgctcttccg atctttatca gatgttctct tgttttaaat c 51
<210> 608
<211> 41
<212> DNA
<213> artificial synthesis
<400> 608
gttcagacgt gtgctcttcc gatctagtag actggaatta g 41
<210> 609
<211> 51
<212> DNA
<213> artificial synthesis
<400> 609
ccctacacga cgctcttccg atctggcaca gcatctgaaa cgtgctggaa t 51
<210> 610
<211> 53
<212> DNA
<213> artificial synthesis
<400> 610
gttcagacgt gtgctcttcc gatctaagtt ttgaccaagt caaagttttc ctc 53
<210> 611
<211> 45
<212> DNA
<213> artificial synthesis
<400> 611
ccctacacga cgctcttccg atctccttgt ctgataactg tatct 45
<210> 612
<211> 45
<212> DNA
<213> artificial synthesis
<400> 612
gttcagacgt gtgctcttcc gatctcctgt accacatgtt atcaa 45
<210> 613
<211> 51
<212> DNA
<213> artificial synthesis
<400> 613
ccctacacga cgctcttccg atctagcatt cttctgcaca gaagtaaaat t 51
<210> 614
<211> 41
<212> DNA
<213> artificial synthesis
<400> 614
gttcagacgt gtgctcttcc gatctaagaa aagtgtctgc a 41
<210> 615
<211> 45
<212> DNA
<213> artificial synthesis
<400> 615
ccctacacga cgctcttccg atctttttgt agaatatcac aatgg 45
<210> 616
<211> 45
<212> DNA
<213> artificial synthesis
<400> 616
gttcagacgt gtgctcttcc gatctccctc cgggcttcta gaatg 45
<210> 617
<211> 42
<212> DNA
<213> artificial synthesis
<400> 617
ccctacacga cgctcttccg atcttgacac aaaagctatt at 42
<210> 618
<211> 42
<212> DNA
<213> artificial synthesis
<400> 618
gttcagacgt gtgctcttcc gatcttgtta taaagcaata tg 42
<210> 619
<211> 48
<212> DNA
<213> artificial synthesis
<400> 619
ccctacacga cgctcttccg atctttagga atattctata cacttact 48
<210> 620
<211> 51
<212> DNA
<213> artificial synthesis
<400> 620
gttcagacgt gtgctcttcc gatctacgca accttgatta tgcctgtttt g 51
<210> 621
<211> 46
<212> DNA
<213> artificial synthesis
<400> 621
ccctacacga cgctcttccg atcttgtgaa atggggacat agcagt 46
<210> 622
<211> 55
<212> DNA
<213> artificial synthesis
<400> 622
gttcagacgt gtgctcttcc gatctcgtgg gaggcagcac tcctcactgc tggca 55
<210> 623
<211> 45
<212> DNA
<213> artificial synthesis
<400> 623
ccctacacga cgctcttccg atctcatttt acagatcagg aaact 45
<210> 624
<211> 44
<212> DNA
<213> artificial synthesis
<400> 624
gttcagacgt gtgctcttcc gatctatcct tcaaaatgat ctta 44
<210> 625
<211> 52
<212> DNA
<213> artificial synthesis
<400> 625
ccctacacga cgctcttccg atctgggaca cagagtcgcg cttttcctag gt 52
<210> 626
<211> 49
<212> DNA
<213> artificial synthesis
<400> 626
gttcagacgt gtgctcttcc gatctacggt ttgggcatgt gagttggcc 49
<210> 627
<211> 53
<212> DNA
<213> artificial synthesis
<400> 627
ccctacacga cgctcttccg atctccgtgc ccggcctgtt tggtttttga tag 53
<210> 628
<211> 48
<212> DNA
<213> artificial synthesis
<400> 628
gttcagacgt gtgctcttcc gatcttaaat gacattcaac aaaacagg 48
<210> 629
<211> 40
<212> DNA
<213> artificial synthesis
<400> 629
ccctacacga cgctcttccg atcttcaatc tactatttaa 40
<210> 630
<211> 44
<212> DNA
<213> artificial synthesis
<400> 630
gttcagacgt gtgctcttcc gatcttttct tgattttcat cgat 44
<210> 631
<211> 55
<212> DNA
<213> artificial synthesis
<400> 631
ccctacacga cgctcttccg atctatcgca gtagttacat cttataaagt cacct 55
<210> 632
<211> 41
<212> DNA
<213> artificial synthesis
<400> 632
gttcagacgt gtgctcttcc gatctaaatg ttgtgaccag a 41
<210> 633
<211> 54
<212> DNA
<213> artificial synthesis
<400> 633
ccctacacga cgctcttccg atctactcga gcgatggaat cagatgatgg agct 54
<210> 634
<211> 56
<212> DNA
<213> artificial synthesis
<400> 634
gttcagacgt gtgctcttcc gatctctgta caatggggat aaaaagagac ttaaca 56
<210> 635
<211> 45
<212> DNA
<213> artificial synthesis
<400> 635
ccctacacga cgctcttccg atctcatggc agatatcacc aattg 45
<210> 636
<211> 51
<212> DNA
<213> artificial synthesis
<400> 636
gttcagacgt gtgctcttcc gatctacctt taccatctct agataaagtt g 51
<210> 637
<211> 53
<212> DNA
<213> artificial synthesis
<400> 637
ccctacacga cgctcttccg atcttcaagt ttgtttctga acctagagat caa 53
<210> 638
<211> 45
<212> DNA
<213> artificial synthesis
<400> 638
gttcagacgt gtgctcttcc gatctaataa aataaagata ggaaa 45
<210> 639
<211> 53
<212> DNA
<213> artificial synthesis
<400> 639
ccctacacga cgctcttccg atctgataaa atccccaggc ctctcccctt ccc 53
<210> 640
<211> 48
<212> DNA
<213> artificial synthesis
<400> 640
gttcagacgt gtgctcttcc gatctgctat gaggcaaaac aactgaga 48
<210> 641
<211> 52
<212> DNA
<213> artificial synthesis
<400> 641
ccctacacga cgctcttccg atctatttat gcgacttggt aattgttgct ac 52
<210> 642
<211> 44
<212> DNA
<213> artificial synthesis
<400> 642
gttcagacgt gtgctcttcc gatctgctcc tcacccaggg agct 44
<210> 643
<211> 55
<212> DNA
<213> artificial synthesis
<400> 643
ccctacacga cgctcttccg atctacgata tcctgattgg ccaggtgcag gtcac 55
<210> 644
<211> 56
<212> DNA
<213> artificial synthesis
<400> 644
gttcagacgt gtgctcttcc gatctcatct ttccattgaa tcttttctcc attttt 56
<210> 645
<211> 43
<212> DNA
<213> artificial synthesis
<400> 645
ccctacacga cgctcttccg atctcctggc ttactggcac act 43
<210> 646
<211> 43
<212> DNA
<213> artificial synthesis
<400> 646
gttcagacgt gtgctcttcc gatctccaga gtcagtcatc tag 43
<210> 647
<211> 42
<212> DNA
<213> artificial synthesis
<400> 647
ccctacacga cgctcttccg atctcattaa tagtataatc ct 42
<210> 648
<211> 55
<212> DNA
<213> artificial synthesis
<400> 648
gttcagacgt gtgctcttcc gatctgtttt tagtcattat tacttgaagt atttc 55
<210> 649
<211> 40
<212> DNA
<213> artificial synthesis
<400> 649
ccctacacga cgctcttccg atctccacct tgggcaacaa 40
<210> 650
<211> 51
<212> DNA
<213> artificial synthesis
<400> 650
gttcagacgt gtgctcttcc gatctcctgc ataattattt gtttaatatt t 51
<210> 651
<211> 53
<212> DNA
<213> artificial synthesis
<400> 651
ccctacacga cgctcttccg atcttgtcat agcaaagaag agcatggaga gac 53
<210> 652
<211> 46
<212> DNA
<213> artificial synthesis
<400> 652
gttcagacgt gtgctcttcc gatctaatta acgaatcaat attcaa 46
<210> 653
<211> 51
<212> DNA
<213> artificial synthesis
<400> 653
ccctacacga cgctcttccg atctcacgaa attgccattt tttgttggtc a 51
<210> 654
<211> 49
<212> DNA
<213> artificial synthesis
<400> 654
gttcagacgt gtgctcttcc gatctaagcc gggcatcaaa gcacatcat 49
<210> 655
<211> 53
<212> DNA
<213> artificial synthesis
<400> 655
ccctacacga cgctcttccg atctaaccca ggctcctcgg cacacgcaga gga 53
<210> 656
<211> 51
<212> DNA
<213> artificial synthesis
<400> 656
gttcagacgt gtgctcttcc gatcttccca gaaggcctgg gagtggggac c 51
<210> 657
<211> 45
<212> DNA
<213> artificial synthesis
<400> 657
ccctacacga cgctcttccg atctgaaaaa ataactttct gtctc 45
<210> 658
<211> 52
<212> DNA
<213> artificial synthesis
<400> 658
gttcagacgt gtgctcttcc gatctcctgc tttggggcag agcctgggtt gg 52
<210> 659
<211> 55
<212> DNA
<213> artificial synthesis
<400> 659
ccctacacga cgctcttccg atctatataa acatgttcat atctcttcca tcaga 55
<210> 660
<211> 46
<212> DNA
<213> artificial synthesis
<400> 660
gttcagacgt gtgctcttcc gatcttcagg ggaatgacag tagcaa 46
<210> 661
<211> 42
<212> DNA
<213> artificial synthesis
<400> 661
ccctacacga cgctcttccg atcttgggga aaggtatgat ga 42
<210> 662
<211> 47
<212> DNA
<213> artificial synthesis
<400> 662
gttcagacgt gtgctcttcc gatctcattt tctttggctc ttcagtc 47
<210> 663
<211> 50
<212> DNA
<213> artificial synthesis
<400> 663
ccctacacga cgctcttccg atcttacttt tttctttgac caccagtttt 50
<210> 664
<211> 51
<212> DNA
<213> artificial synthesis
<400> 664
gttcagacgt gtgctcttcc gatctagagg atacatgttt gaaaactaaa g 51
<210> 665
<211> 45
<212> DNA
<213> artificial synthesis
<400> 665
ccctacacga cgctcttccg atctgccgca gctcactgtg attct 45
<210> 666
<211> 49
<212> DNA
<213> artificial synthesis
<400> 666
gttcagacgt gtgctcttcc gatctaggct gggaagggga gtaacccca 49
<210> 667
<211> 42
<212> DNA
<213> artificial synthesis
<400> 667
ccctacacga cgctcttccg atctagtctg tatgtatcat aa 42
<210> 668
<211> 52
<212> DNA
<213> artificial synthesis
<400> 668
gttcagacgt gtgctcttcc gatctagaat gagaaaatag aaaagcttga ag 52
<210> 669
<211> 53
<212> DNA
<213> artificial synthesis
<400> 669
ccctacacga cgctcttccg atcttaggtg gcacaaggtg agatctacct ggc 53
<210> 670
<211> 42
<212> DNA
<213> artificial synthesis
<400> 670
gttcagacgt gtgctcttcc gatcttcttt tatcgtgatg ca 42
<210> 671
<211> 54
<212> DNA
<213> artificial synthesis
<400> 671
ccctacacga cgctcttccg atctaaagcg ctaggattac aggcatgagc cacc 54
<210> 672
<211> 46
<212> DNA
<213> artificial synthesis
<400> 672
gttcagacgt gtgctcttcc gatctggtac cagtccgtgg cccggg 46
<210> 673
<211> 47
<212> DNA
<213> artificial synthesis
<400> 673
ccctacacga cgctcttccg atcttctccc catggcacac acagtca 47
<210> 674
<211> 41
<212> DNA
<213> artificial synthesis
<400> 674
gttcagacgt gtgctcttcc gatctgagga gctttgtatg t 41
<210> 675
<211> 53
<212> DNA
<213> artificial synthesis
<400> 675
ccctacacga cgctcttccg atctccaggc cctccaaaac agctcataat ata 53
<210> 676
<211> 46
<212> DNA
<213> artificial synthesis
<400> 676
gttcagacgt gtgctcttcc gatcttgtgt tggtggaggg gatagt 46
<210> 677
<211> 48
<212> DNA
<213> artificial synthesis
<400> 677
ccctacacga cgctcttccg atcttctgtt tacccatggg actctgtc 48
<210> 678
<211> 43
<212> DNA
<213> artificial synthesis
<400> 678
gttcagacgt gtgctcttcc gatctacagc ctgcattcga atg 43
<210> 679
<211> 39
<212> DNA
<213> artificial synthesis
<400> 679
ccctacacga cgctcttccg atctattttt caaggggaa 39
<210> 680
<211> 44
<212> DNA
<213> artificial synthesis
<400> 680
gttcagacgt gtgctcttcc gatctattgg taggatttgc aact 44
<210> 681
<211> 53
<212> DNA
<213> artificial synthesis
<400> 681
ccctacacga cgctcttccg atctaccagt agagccacct ttattagcac aaa 53
<210> 682
<211> 53
<212> DNA
<213> artificial synthesis
<400> 682
gttcagacgt gtgctcttcc gatcttcatt tctatttatc tcccagtgta act 53
<210> 683
<211> 40
<212> DNA
<213> artificial synthesis
<400> 683
ccctacacga cgctcttccg atctatctca aaaacattat 40
<210> 684
<211> 43
<212> DNA
<213> artificial synthesis
<400> 684
gttcagacgt gtgctcttcc gatcttcact cctcagttcc cct 43
<210> 685
<211> 40
<212> DNA
<213> artificial synthesis
<400> 685
ccctacacga cgctcttccg atctatttgc aatatggaat 40
<210> 686
<211> 43
<212> DNA
<213> artificial synthesis
<400> 686
gttcagacgt gtgctcttcc gatctattat atgccaaaaa tgt 43
<210> 687
<211> 51
<212> DNA
<213> artificial synthesis
<400> 687
ccctacacga cgctcttccg atctgtaact tccagttcca gcagatcttc c 51
<210> 688
<211> 47
<212> DNA
<213> artificial synthesis
<400> 688
gttcagacgt gtgctcttcc gatctgaaaa tattaaaagt ataagat 47
<210> 689
<211> 41
<212> DNA
<213> artificial synthesis
<400> 689
ccctacacga cgctcttccg atctgctcac tgggcagcag t 41
<210> 690
<211> 43
<212> DNA
<213> artificial synthesis
<400> 690
gttcagacgt gtgctcttcc gatctaataa tttctagaac att 43
<210> 691
<211> 50
<212> DNA
<213> artificial synthesis
<400> 691
ccctacacga cgctcttccg atcttcaggc ctcgccactg gccagctggg 50
<210> 692
<211> 53
<212> DNA
<213> artificial synthesis
<400> 692
gttcagacgt gtgctcttcc gatctctagg cagtaacaca gtgccaagtg ctc 53
<210> 693
<211> 41
<212> DNA
<213> artificial synthesis
<400> 693
ccctacacga cgctcttccg atctaagatt tacccagtgt a 41
<210> 694
<211> 56
<212> DNA
<213> artificial synthesis
<400> 694
gttcagacgt gtgctcttcc gatctcagga ccacaaactt cacattgttt gagttc 56
<210> 695
<211> 44
<212> DNA
<213> artificial synthesis
<400> 695
ccctacacga cgctcttccg atctcgcttt tggaaatgtg aaat 44
<210> 696
<211> 41
<212> DNA
<213> artificial synthesis
<400> 696
gttcagacgt gtgctcttcc gatctcttac tcttttttct t 41
<210> 697
<211> 49
<212> DNA
<213> artificial synthesis
<400> 697
ccctacacga cgctcttccg atctcatcat gagttggact cctcccttg 49
<210> 698
<211> 44
<212> DNA
<213> artificial synthesis
<400> 698
gttcagacgt gtgctcttcc gatcttaacc tctcaatgga ataa 44
<210> 699
<211> 44
<212> DNA
<213> artificial synthesis
<400> 699
ccctacacga cgctcttccg atctgaccaa acctagacag gaat 44
<210> 700
<211> 50
<212> DNA
<213> artificial synthesis
<400> 700
gttcagacgt gtgctcttcc gatctaagta agaagtccat gactaaatag 50
<210> 701
<211> 47
<212> DNA
<213> artificial synthesis
<400> 701
ccctacacga cgctcttccg atctcaatta taaaataggc aaatgct 47
<210> 702
<211> 56
<212> DNA
<213> artificial synthesis
<400> 702
gttcagacgt gtgctcttcc gatcttacca gacttacaca agaaaatgca actcct 56
<210> 703
<211> 53
<212> DNA
<213> artificial synthesis
<400> 703
ccctacacga cgctcttccg atctctactg tgcccacaca ccccttcaaa tat 53
<210> 704
<211> 44
<212> DNA
<213> artificial synthesis
<400> 704
gttcagacgt gtgctcttcc gatctgacct gttcattctt tgtt 44
<210> 705
<211> 40
<212> DNA
<213> artificial synthesis
<400> 705
ccctacacga cgctcttccg atctaatttg ctcagtatat 40
<210> 706
<211> 48
<212> DNA
<213> artificial synthesis
<400> 706
gttcagacgt gtgctcttcc gatctaatta gacaccatat gtgggatg 48
<210> 707
<211> 52
<212> DNA
<213> artificial synthesis
<400> 707
ccctacacga cgctcttccg atctaagatt tggagatata tatggtttcc at 52
<210> 708
<211> 50
<212> DNA
<213> artificial synthesis
<400> 708
gttcagacgt gtgctcttcc gatctatgtg tgttgtgtgt gtgtgtgtat 50
<210> 709
<211> 48
<212> DNA
<213> artificial synthesis
<400> 709
ccctacacga cgctcttccg atctcagcga gaggctccat ttcagcct 48
<210> 710
<211> 45
<212> DNA
<213> artificial synthesis
<400> 710
gttcagacgt gtgctcttcc gatctgattt aggccctatt tataa 45
<210> 711
<211> 52
<212> DNA
<213> artificial synthesis
<400> 711
ccctacacga cgctcttccg atcttctagt ttgaaggaag gaatgttggt ag 52
<210> 712
<211> 47
<212> DNA
<213> artificial synthesis
<400> 712
gttcagacgt gtgctcttcc gatcttggac agagagggtg taggtta 47
<210> 713
<211> 48
<212> DNA
<213> artificial synthesis
<400> 713
ccctacacga cgctcttccg atctggtttt gatatacaca gacacagt 48
<210> 714
<211> 54
<212> DNA
<213> artificial synthesis
<400> 714
gttcagacgt gtgctcttcc gatcttgaga atatagaaat tttcacaaat atta 54
<210> 715
<211> 42
<212> DNA
<213> artificial synthesis
<400> 715
ccctacacga cgctcttccg atctggggag ggtgggatcc ta 42
<210> 716
<211> 48
<212> DNA
<213> artificial synthesis
<400> 716
gttcagacgt gtgctcttcc gatcttgtgc acggtgcaga cagcagtg 48
<210> 717
<211> 50
<212> DNA
<213> artificial synthesis
<400> 717
ccctacacga cgctcttccg atctcctgct gtgatattgc ccaggcgggg 50
<210> 718
<211> 48
<212> DNA
<213> artificial synthesis
<400> 718
gttcagacgt gtgctcttcc gatctacaaa aatcattggc ctctgtag 48
<210> 719
<211> 46
<212> DNA
<213> artificial synthesis
<400> 719
ccctacacga cgctcttccg atctctgaac acatgctaag tggcat 46
<210> 720
<211> 56
<212> DNA
<213> artificial synthesis
<400> 720
gttcagacgt gtgctcttcc gatctattct tccattatcg cttagtggga atcggt 56
<210> 721
<211> 40
<212> DNA
<213> artificial synthesis
<400> 721
ccctacacga cgctcttccg atcttaaggt agtagagaga 40
<210> 722
<211> 43
<212> DNA
<213> artificial synthesis
<400> 722
gttcagacgt gtgctcttcc gatctgcatc ggcatgtaaa att 43
<210> 723
<211> 52
<212> DNA
<213> artificial synthesis
<400> 723
ccctacacga cgctcttccg atctatggat acctatctga ggtatatcat ta 52
<210> 724
<211> 47
<212> DNA
<213> artificial synthesis
<400> 724
gttcagacgt gtgctcttcc gatcttatgt tcattagaca taattta 47
<210> 725
<211> 55
<212> DNA
<213> artificial synthesis
<400> 725
ccctacacga cgctcttccg atctagacca ggaaaatttg aaaaactatt ggatg 55
<210> 726
<211> 51
<212> DNA
<213> artificial synthesis
<400> 726
gttcagacgt gtgctcttcc gatcttaaga ataacctttt taccttatct t 51
<210> 727
<211> 40
<212> DNA
<213> artificial synthesis
<400> 727
ccctacacga cgctcttccg atcttccacc aggtagaaac 40
<210> 728
<211> 52
<212> DNA
<213> artificial synthesis
<400> 728
gttcagacgt gtgctcttcc gatctcattt ccttgaccac aatgattgtt ca 52
<210> 729
<211> 40
<212> DNA
<213> artificial synthesis
<400> 729
ccctacacga cgctcttccg atctaggcgt gagccaccac 40
<210> 730
<211> 43
<212> DNA
<213> artificial synthesis
<400> 730
gttcagacgt gtgctcttcc gatctccaaa gaccaaactg ttg 43
<210> 731
<211> 49
<212> DNA
<213> artificial synthesis
<400> 731
ccctacacga cgctcttccg atcttgttgt tgtttgtttg tttatttta 49
<210> 732
<211> 55
<212> DNA
<213> artificial synthesis
<400> 732
gttcagacgt gtgctcttcc gatctgtgaa tgcttcctcc aacttcctga gagca 55
<210> 733
<211> 48
<212> DNA
<213> artificial synthesis
<400> 733
ccctacacga cgctcttccg atctttttaa caaacccctg taatgttc 48
<210> 734
<211> 44
<212> DNA
<213> artificial synthesis
<400> 734
gttcagacgt gtgctcttcc gatctcaagc aacttgacct tgat 44
<210> 735
<211> 44
<212> DNA
<213> artificial synthesis
<400> 735
ccctacacga cgctcttccg atctacttga ccacagtgct atct 44
<210> 736
<211> 47
<212> DNA
<213> artificial synthesis
<400> 736
gttcagacgt gtgctcttcc gatcttctca gatgagaact tgtatct 47
<210> 737
<211> 45
<212> DNA
<213> artificial synthesis
<400> 737
ccctacacga cgctcttccg atctttcctt atgctttaat ttggc 45
<210> 738
<211> 42
<212> DNA
<213> artificial synthesis
<400> 738
gttcagacgt gtgctcttcc gatctggagc aaagagctta aa 42
<210> 739
<211> 40
<212> DNA
<213> artificial synthesis
<400> 739
ccctacacga cgctcttccg atctaataat aattcttcat 40
<210> 740
<211> 47
<212> DNA
<213> artificial synthesis
<400> 740
gttcagacgt gtgctcttcc gatctccctt ttagagataa ctcactc 47
<210> 741
<211> 49
<212> DNA
<213> artificial synthesis
<400> 741
ccctacacga cgctcttccg atctttactc tcaacaactg tcttaatcc 49
<210> 742
<211> 49
<212> DNA
<213> artificial synthesis
<400> 742
gttcagacgt gtgctcttcc gatctagcta aacatcactt ttaggaatt 49
<210> 743
<211> 45
<212> DNA
<213> artificial synthesis
<400> 743
ccctacacga cgctcttccg atctaagaca tcatgaagag aatct 45
<210> 744
<211> 48
<212> DNA
<213> artificial synthesis
<400> 744
gttcagacgt gtgctcttcc gatcttctgc aaactcatga acccagca 48
<210> 745
<211> 55
<212> DNA
<213> artificial synthesis
<400> 745
ccctacacga cgctcttccg atctcaaaat gagggcagaa acatttgatt attta 55
<210> 746
<211> 49
<212> DNA
<213> artificial synthesis
<400> 746
gttcagacgt gtgctcttcc gatctaaact ctaagaaatt ataaagatt 49
<210> 747
<211> 44
<212> DNA
<213> artificial synthesis
<400> 747
ccctacacga cgctcttccg atctacagtt tcccctttgt catt 44
<210> 748
<211> 45
<212> DNA
<213> artificial synthesis
<400> 748
gttcagacgt gtgctcttcc gatctccctc agggcaggtg cctct 45
<210> 749
<211> 53
<212> DNA
<213> artificial synthesis
<400> 749
ccctacacga cgctcttccg atctactaaa gtgaggagct ctcactgctg ggc 53
<210> 750
<211> 48
<212> DNA
<213> artificial synthesis
<400> 750
gttcagacgt gtgctcttcc gatctctgac ctcgtgatcc gcccgcct 48
<210> 751
<211> 47
<212> DNA
<213> artificial synthesis
<400> 751
ccctacacga cgctcttccg atctaatcaa gtagataaca agtgtta 47
<210> 752
<211> 54
<212> DNA
<213> artificial synthesis
<400> 752
gttcagacgt gtgctcttcc gatctaaatg aatgcaaaca tagttgggct acat 54
<210> 753
<211> 51
<212> DNA
<213> artificial synthesis
<400> 753
ccctacacga cgctcttccg atctatccac ttatatttga ttcatttcat a 51
<210> 754
<211> 50
<212> DNA
<213> artificial synthesis
<400> 754
gttcagacgt gtgctcttcc gatcttagat cctgcaacaa aacggcaatg 50
<210> 755
<211> 40
<212> DNA
<213> artificial synthesis
<400> 755
ccctacacga cgctcttccg atctaacaca tttatctgag 40
<210> 756
<211> 55
<212> DNA
<213> artificial synthesis
<400> 756
gttcagacgt gtgctcttcc gatcttgggg ttgaggaagt ggtgcacggc cagaa 55
<210> 757
<211> 54
<212> DNA
<213> artificial synthesis
<400> 757
ccctacacga cgctcttccg atctgtatct gttttattaa tcattttatc cctg 54
<210> 758
<211> 55
<212> DNA
<213> artificial synthesis
<400> 758
gttcagacgt gtgctcttcc gatcttctgt gggacgggaa agacagtgac tgagg 55
<210> 759
<211> 49
<212> DNA
<213> artificial synthesis
<400> 759
ccctacacga cgctcttccg atcttggcag gctggtctcg aactcctga 49
<210> 760
<211> 47
<212> DNA
<213> artificial synthesis
<400> 760
gttcagacgt gtgctcttcc gatctatgct tctcaaatct tggtgtc 47
<210> 761
<211> 41
<212> DNA
<213> artificial synthesis
<400> 761
ccctacacga cgctcttccg atcttcctgc aagggccggg a 41
<210> 762
<211> 53
<212> DNA
<213> artificial synthesis
<400> 762
gttcagacgt gtgctcttcc gatctctttc gacataagtg gtcacgggcg gaa 53
<210> 763
<211> 45
<212> DNA
<213> artificial synthesis
<400> 763
ccctacacga cgctcttccg atctacttaa agtataattt tttta 45
<210> 764
<211> 56
<212> DNA
<213> artificial synthesis
<400> 764
gttcagacgt gtgctcttcc gatctaatac atctgtcctc gccatttcta ttcaat 56
<210> 765
<211> 51
<212> DNA
<213> artificial synthesis
<400> 765
ccctacacga cgctcttccg atcttaacat gccattgttc atactggtaa t 51
<210> 766
<211> 42
<212> DNA
<213> artificial synthesis
<400> 766
gttcagacgt gtgctcttcc gatcttacaa caaatcatat ta 42
<210> 767
<211> 43
<212> DNA
<213> artificial synthesis
<400> 767
ccctacacga cgctcttccg atctcgtcat tgtaactgtg gag 43
<210> 768
<211> 53
<212> DNA
<213> artificial synthesis
<400> 768
gttcagacgt gtgctcttcc gatcttccta atgacaagtt tctcagatca cat 53
<210> 769
<211> 48
<212> DNA
<213> artificial synthesis
<400> 769
ccctacacga cgctcttccg atctttcaca cacagtatga acttaaca 48
<210> 770
<211> 55
<212> DNA
<213> artificial synthesis
<400> 770
gttcagacgt gtgctcttcc gatcttgtac acacatacac acacatatag aaata 55
<210> 771
<211> 42
<212> DNA
<213> artificial synthesis
<400> 771
ccctacacga cgctcttccg atcttttcac atttcttcct ca 42
<210> 772
<211> 50
<212> DNA
<213> artificial synthesis
<400> 772
gttcagacgt gtgctcttcc gatctaattt agtgctgaat tatgcattca 50
<210> 773
<211> 43
<212> DNA
<213> artificial synthesis
<400> 773
ccctacacga cgctcttccg atctgacagc cccgtacaca agg 43
<210> 774
<211> 53
<212> DNA
<213> artificial synthesis
<400> 774
gttcagacgt gtgctcttcc gatctgggaa cgtgcgggac ccaccgtcct ggc 53
<210> 775
<211> 43
<212> DNA
<213> artificial synthesis
<400> 775
ccctacacga cgctcttccg atctgacagc cccgtacaca agg 43
<210> 776
<211> 53
<212> DNA
<213> artificial synthesis
<400> 776
gttcagacgt gtgctcttcc gatctgggaa cgtgcgggac ccaccgtcct ggc 53
<210> 777
<211> 46
<212> DNA
<213> artificial synthesis
<400> 777
ccctacacga cgctcttccg atctcttagc accctgatcc tgcagc 46
<210> 778
<211> 49
<212> DNA
<213> artificial synthesis
<400> 778
gttcagacgt gtgctcttcc gatctcacag ggaggcagat gcagtgggt 49
<210> 779
<211> 42
<212> DNA
<213> artificial synthesis
<400> 779
ccctacacga cgctcttccg atcttccagg caccttctct cc 42
<210> 780
<211> 43
<212> DNA
<213> artificial synthesis
<400> 780
gttcagacgt gtgctcttcc gatctcatgg agctctttgt acc 43
<210> 781
<211> 53
<212> DNA
<213> artificial synthesis
<400> 781
ccctacacga cgctcttccg atctccctct gataatgctg atgctgatgc agg 53
<210> 782
<211> 43
<212> DNA
<213> artificial synthesis
<400> 782
gttcagacgt gtgctcttcc gatctctcct ccagccattg aag 43
<210> 783
<211> 44
<212> DNA
<213> artificial synthesis
<400> 783
ccctacacga cgctcttccg atctcagatg ttcacagatt tctc 44
<210> 784
<211> 47
<212> DNA
<213> artificial synthesis
<400> 784
gttcagacgt gtgctcttcc gatcttccta gacttagaat attttat 47
<210> 785
<211> 45
<212> DNA
<213> artificial synthesis
<400> 785
ccctacacga cgctcttccg atctgattac atcacatagt tcccc 45
<210> 786
<211> 55
<212> DNA
<213> artificial synthesis
<400> 786
gttcagacgt gtgctcttcc gatctgcagc gacaggagaa aagagaaaag gaagc 55
<210> 787
<211> 44
<212> DNA
<213> artificial synthesis
<400> 787
ccctacacga cgctcttccg atctttcctc attgtcactt ttcc 44
<210> 788
<211> 52
<212> DNA
<213> artificial synthesis
<400> 788
gttcagacgt gtgctcttcc gatctagacc gcccacttgg atatcctcat tc 52
<210> 789
<211> 40
<212> DNA
<213> artificial synthesis
<400> 789
ccctacacga cgctcttccg atctgttcac caggttttga 40
<210> 790
<211> 49
<212> DNA
<213> artificial synthesis
<400> 790
gttcagacgt gtgctcttcc gatctctgtg agaacaagga acttgttta 49
<210> 791
<211> 42
<212> DNA
<213> artificial synthesis
<400> 791
ccctacacga cgctcttccg atcttttgcc aatgcttcct at 42
<210> 792
<211> 47
<212> DNA
<213> artificial synthesis
<400> 792
gttcagacgt gtgctcttcc gatctaaaac aacttttgac cagagaa 47
<210> 793
<211> 50
<212> DNA
<213> artificial synthesis
<400> 793
ccctacacga cgctcttccg atcttgaatg tttggaaaca cacagtgtgg 50
<210> 794
<211> 40
<212> DNA
<213> artificial synthesis
<400> 794
gttcagacgt gtgctcttcc gatcttcctg tctacctcac 40
<210> 795
<211> 46
<212> DNA
<213> artificial synthesis
<400> 795
ccctacacga cgctcttccg atctaagtga tactttttat gtctta 46
<210> 796
<211> 55
<212> DNA
<213> artificial synthesis
<400> 796
gttcagacgt gtgctcttcc gatctctcaa gaagggctgc tgtctagatt ccttg 55
<210> 797
<211> 41
<212> DNA
<213> artificial synthesis
<400> 797
ccctacacga cgctcttccg atctaatgaa ctactggtac t 41
<210> 798
<211> 41
<212> DNA
<213> artificial synthesis
<400> 798
gttcagacgt gtgctcttcc gatctccttc tggtatctat c 41
<210> 799
<211> 49
<212> DNA
<213> artificial synthesis
<400> 799
ccctacacga cgctcttccg atctgcatgg gggggacaga cactgccag 49
<210> 800
<211> 41
<212> DNA
<213> artificial synthesis
<400> 800
gttcagacgt gtgctcttcc gatctgtcat ggcagcaggt g 41
<210> 801
<211> 42
<212> DNA
<213> artificial synthesis
<400> 801
ccctacacga cgctcttccg atctcttggc atttctgaga ct 42
<210> 802
<211> 49
<212> DNA
<213> artificial synthesis
<400> 802
gttcagacgt gtgctcttcc gatcttctgc agggctggag ccattcctc 49
<210> 803
<211> 46
<212> DNA
<213> artificial synthesis
<400> 803
ccctacacga cgctcttccg atcttgttaa tttatatttt cttact 46
<210> 804
<211> 48
<212> DNA
<213> artificial synthesis
<400> 804
gttcagacgt gtgctcttcc gatctgactg atatttttta atcaacaa 48
<210> 805
<211> 43
<212> DNA
<213> artificial synthesis
<400> 805
ccctacacga cgctcttccg atctagattt ttctaaacat tgt 43
<210> 806
<211> 41
<212> DNA
<213> artificial synthesis
<400> 806
gttcagacgt gtgctcttcc gatcttaagc cattgcccgg c 41
Claims (4)
1.一种基于多重PCR的二代测序的建库方法，其特征在于，包括：
1）、提取样本DNA；
2）、基于提取的样本DNA，配置第一步PCR扩增体系，进行第一步PCR扩增并纯化，
3）、基于第一步PCR扩增并纯化后的纯化产物进行第二次PCR扩增并纯化；
4）、对第二次PCR扩增并纯化后的纯化产物进行电泳鉴定后进行测序分析；
第一步PCR扩增体系包括：25体积份的Platinum Multiplex PCR Master Mix 2×、5体积份的GC Enhancer、15体积份的引物组合和5体积份的DNA样本；
第二步PCR扩增体系包括：25体积份的2×KAPA HiFi Hotstart Ready Mix、1体积份的PE1.0、1体积份的Barcode和23体积份的第一步PCR扩增纯化产物；
引物组合包括：如SEQ ID NO:1-SEQ ID NO:806所示核苷酸序列；
所述第一步PCR扩增的条件为：98℃、3分钟，循环一次；96℃、45秒，80℃、30秒，54℃、3分钟，68℃、30秒，循环45次；
所述第二次PCR扩增的条件为：98℃、3分钟，循环一次；98℃、20秒，65℃、30秒，72℃、20秒，循环15次；68℃保持10分钟。
2.如权利要求1所述基于多重PCR的二代测序的建库方法，其特征在于，所述电泳鉴定的条带长度在100-200bp之间。
3.如权利要求1所述基于多重PCR的二代测序的建库方法，其特征在于，所述测序分析采用HiSeq2000测序平台进行测序，上靶率高于98％，平均深度深于985×，98.9％的位点测序深度在10×以上。
4.如权利要求1所述基于多重PCR的二代测序的建库方法，其特征在于，所述样本DNA来源于外周静脉血、组织或鼻咽拭子样本。
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