CN114606168B - Composite microbial inoculum and composition for ecological restoration, and preparation method and application thereof - Google Patents
Composite microbial inoculum and composition for ecological restoration, and preparation method and application thereof Download PDFInfo
- Publication number
- CN114606168B CN114606168B CN202210447769.2A CN202210447769A CN114606168B CN 114606168 B CN114606168 B CN 114606168B CN 202210447769 A CN202210447769 A CN 202210447769A CN 114606168 B CN114606168 B CN 114606168B
- Authority
- CN
- China
- Prior art keywords
- composite microbial
- composition
- ecological restoration
- soil
- microbial inoculum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000002131 composite material Substances 0.000 title claims abstract description 68
- 239000000203 mixture Substances 0.000 title claims abstract description 51
- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 41
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 239000002689 soil Substances 0.000 claims abstract description 38
- 230000000813 microbial effect Effects 0.000 claims abstract description 32
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 31
- 230000035784 germination Effects 0.000 claims abstract description 17
- 238000009825 accumulation Methods 0.000 claims abstract description 11
- 241000194108 Bacillus licheniformis Species 0.000 claims description 25
- 241001540751 Talaromyces ruber Species 0.000 claims description 24
- 241000228212 Aspergillus Species 0.000 claims description 22
- 230000001737 promoting effect Effects 0.000 claims description 21
- 235000017388 Geotrichum candidum Nutrition 0.000 claims description 19
- 241000187747 Streptomyces Species 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 13
- 241000235395 Mucor Species 0.000 claims description 9
- 241001313536 Thermothelomyces thermophila Species 0.000 claims description 9
- 241001136487 Eurotium Species 0.000 claims description 8
- 241000894006 Bacteria Species 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 6
- 150000003839 salts Chemical class 0.000 claims description 6
- 239000000589 Siderophore Substances 0.000 claims description 5
- 241000499912 Trichoderma reesei Species 0.000 claims description 5
- 239000004016 soil organic matter Substances 0.000 claims description 5
- 206010006500 Brucellosis Diseases 0.000 claims description 4
- 241001655322 Streptomycetales Species 0.000 claims description 4
- 239000007766 cera flava Substances 0.000 claims description 4
- 230000008635 plant growth Effects 0.000 claims description 4
- 241000132177 Aspergillus glaucus Species 0.000 claims description 3
- 241000186146 Brevibacterium Species 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 241000453701 Galactomyces candidum Species 0.000 claims 3
- 241000669761 Aggregata Species 0.000 claims 1
- 241000159512 Geotrichum Species 0.000 claims 1
- 241000235527 Rhizopus Species 0.000 claims 1
- 238000007605 air drying Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 244000005700 microbiome Species 0.000 abstract description 23
- 230000009286 beneficial effect Effects 0.000 abstract description 7
- 238000011084 recovery Methods 0.000 abstract description 7
- 238000013329 compounding Methods 0.000 abstract description 4
- 238000003012 network analysis Methods 0.000 abstract description 3
- 239000005416 organic matter Substances 0.000 abstract description 3
- 150000001875 compounds Chemical class 0.000 abstract description 2
- 239000001963 growth medium Substances 0.000 description 54
- 238000012258 culturing Methods 0.000 description 29
- 230000000052 comparative effect Effects 0.000 description 21
- 238000009630 liquid culture Methods 0.000 description 18
- 244000168141 Geotrichum candidum Species 0.000 description 16
- 235000015097 nutrients Nutrition 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 7
- 241000219782 Sesbania Species 0.000 description 5
- 239000002028 Biomass Substances 0.000 description 4
- 241000226677 Myceliophthora Species 0.000 description 4
- 244000061456 Solanum tuberosum Species 0.000 description 4
- 235000002595 Solanum tuberosum Nutrition 0.000 description 4
- 241001600136 Streptomyces thermotolerans Species 0.000 description 4
- 239000012615 aggregate Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 238000009331 sowing Methods 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 238000009631 Broth culture Methods 0.000 description 3
- BZUNJUAMQZRJIP-UHFFFAOYSA-N CPDA Natural products OCCCCCCCCCCCCCCC(O)=O BZUNJUAMQZRJIP-UHFFFAOYSA-N 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 235000013882 gravy Nutrition 0.000 description 3
- GISJHCLTIVIGLX-UHFFFAOYSA-N n-[4-[(4-chlorophenyl)methoxy]pyridin-2-yl]-2-(2,6-difluorophenyl)acetamide Chemical compound FC1=CC=CC(F)=C1CC(=O)NC1=CC(OCC=2C=CC(Cl)=CC=2)=CC=N1 GISJHCLTIVIGLX-UHFFFAOYSA-N 0.000 description 3
- 230000001902 propagating effect Effects 0.000 description 3
- 238000007873 sieving Methods 0.000 description 3
- 241001205401 Aspergillus cristatus Species 0.000 description 2
- 241000134912 Aspergillus penicillioides Species 0.000 description 2
- 241000030451 Byssochlamys fulva Species 0.000 description 2
- 241001428407 Ceramium Species 0.000 description 2
- 241000588748 Klebsiella Species 0.000 description 2
- 241001116661 Labrenzia aggregata Species 0.000 description 2
- 241000235575 Mortierella Species 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- KMUONIBRACKNSN-UHFFFAOYSA-N potassium dichromate Chemical compound [K+].[K+].[O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O KMUONIBRACKNSN-UHFFFAOYSA-N 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 241000606153 Chlamydia trachomatis Species 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000001640 apoptogenic effect Effects 0.000 description 1
- 229940038705 chlamydia trachomatis Drugs 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000006870 ms-medium Substances 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000002688 soil aggregate Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention relates to the technical field of ecological restoration, in particular to a composite microbial inoculum and a composite microbial inoculum composition for ecological restoration, and a preparation method and application thereof. The invention collects typical coastal zone plant and soil samples in north of China, determines microorganisms, and performs network analysis on the combination of plant and soil microorganisms to analyze a core microbiome; the compound microbial agent obtained by compounding different microorganisms is not only beneficial to the establishment of degraded tidal flat vegetation, but also has high germination rate, can promote the accumulation of soil organic matters, and has low recovery cost. According to the embodiment of the invention, compared with a control group without the composite microbial inoculum, the germination rate of the test group with the composite microbial inoculum is improved by 17.6%, and the organic matter content of soil is improved by 41.5%.
Description
Technical Field
The invention relates to the technical field of ecological restoration, in particular to a composite microbial inoculum and a composite microbial inoculum composition for ecological restoration, and a preparation method and application thereof.
Background
The degraded beach of the northern coastal zone is a key problem of obstructing ecological restoration because soil organic matters are easy to run off and difficult to accumulate besides saline-alkali stress, environmental pollution and artificial activity interference obstructing plant growth and vegetation establishment. The vegetation is difficult to build and the soil organic matters are difficult to accumulate, and the vegetation and the soil organic matters are mutually influenced, namely, the vegetation is built with low soil organic matters, if the vegetation cannot be built, nutrients cannot be returned to the soil, and the soil organic matters cannot be increased. The traditional vegetation recovery is mainly based on direct forestation, and main measures include soil covering, organic fertilizer adding, fixing and the like, so that the forestation cost is high and the germination rate is low.
Disclosure of Invention
In order to solve the problems, the invention provides a composite microbial inoculum and a composition for ecological restoration, and a preparation method and application thereof. The composite microbial inoculum provided by the invention is not only beneficial to the establishment of degraded tidal flat vegetation, but also has high germination rate, can promote the accumulation of soil organic matters, and has low recovery cost.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a composite microbial agent for ecological restoration, which comprises the following components: bacillus licheniformis (Bacillus licheniformis), bullezia collecticola (Labrenzia aggregata), streptomyces thermodurus (Streptomyces thermotolerans), aspergillus broodstock (Aspergillus penicillioides), mucor pulmonale (Byssochlamys fulva), penicillium purpurogenum (Penicillium purpurogenum), mortierella thermophila (Sporotrichum thermophile), geotrichum candidum (Geotrichum candidum) and Ceramium She Sannang (Eurotium herbariorum);
the viable count of the bacillus licheniformis and the brucellosis agglomerans is respectively more than 1 multiplied by 10 8 CUF/mL or 1X 10 8 CUF/g; the number of viable bacteria of the heat-resistant streptomycete, the aspergillus broodstock, the pure trichoderma reesei, the penicillium purpurogenum, the siderophore thermophilus, the geotrichum candidum and the Eurotium cereum is respectively more than 1 multiplied by 10 6 CUF/mL or 1X 10 6 CUF/g。
Preferably, when the composite microbial agent is a liquid, the composite microbial agent comprises the following components in parts by volume: bacillus licheniformis, bulletia glottalis, streptomyces thermodurus, aspergillus broodstocus, mucor pulmonale, penicillium purpurogenum, sporotrichum thermophilum, geotrichum candidum and Cera flava She Sannang;
when the composite microbial inoculum is solid, the composite microbial inoculum comprises the following components in parts by mass: bacillus licheniformis, brevibacterium glomerata, streptomyces thermodurus, aspergillus broodstocus, mucor pulmonale, penicillium purpurogenum, sporotrichum thermophilum, geotrichum candidum and Eurotium cereum.
The invention also provides a composition for ecological restoration, which comprises the composite microbial inoculum and biochar.
Preferably, the mass ratio of the composite microbial inoculum to the biochar is (0.5-3): 100.
the invention also provides a preparation method of the composition, which comprises the following steps: and mixing the composite microbial inoculum with biochar to obtain the composition.
Preferably, when the composite microbial inoculum is liquid, the composite microbial inoculum is further dried at a temperature lower than 40 ℃ after being mixed to obtain the composition.
The invention also provides application of the composite microbial inoculum or the composition prepared by the preparation method in promoting ecological restoration of degraded tidal flat.
Preferably, the ecological restoration includes promoting vegetation establishment and/or promoting soil organic matter accumulation.
Preferably, the ecological restoration area comprises an area with 3-8%o of salt content in soil.
The invention also provides a method for promoting the ecological restoration of the degraded tidal flat, which comprises the following steps: mixing the composition with soil of the area to be restored.
The beneficial effects are that:
the invention provides a composite microbial agent for ecological restoration, which comprises the following components: bacillus licheniformis (Bacillus licheniformis), bullezia collecticola (Labrenzia aggregata), streptomyces thermodurus (Streptomyces thermotolerans), aspergillus broodstock (Aspergillus penicillioides), mucor pulmonale (Byssochlamys fulva), penicillium purpurogenum (Penicillium purpurogenum), mortierella thermophila (Sporotrichum thermophile), geotrichum candidum (Geotrichum candidum) and Ceramium She Sannang (Eurotium herbariorum); the viable count of the bacillus licheniformis and the brucellosis agglomerans is respectively more than 1 multiplied by 10 8 CUF/mL or 1X 10 8 CUF/g; the number of viable bacteria of the heat-resistant streptomycete, the aspergillus broodstock, the pure trichoderma reesei, the penicillium purpurogenum, the siderophore thermophilus, the geotrichum candidum and the Eurotium cereum is respectively more than 1 multiplied by 10 6 CUF/mL or 1X 10 6 CUF/g. The invention collects typical coastal zone plant and soil samples in north of China, determines microorganisms, and performs network analysis on the combination of plant and soil microorganisms to analyze a core microbiome; the compound microbial agent obtained by compounding different microorganisms is not only beneficial to the establishment of degraded tidal flat vegetation, but also has high germination rate, can promote the accumulation of soil organic matters, and has low recovery cost. According to the embodiment of the invention, compared with a control group without the composite microbial inoculum, the germination rate of the test group with the composite microbial inoculum is improved by 17.6%, and the organic matter content of soil is improved by 41.5%.
Detailed Description
The invention provides a composite microbial agent for ecological restoration, which comprises the following components: bacillus licheniformis, bulletia glottalis, streptomyces thermodurus, aspergillus broodstocus, mucor pulmonale, penicillium purpurogenum, sporotrichum thermophilum, geotrichum candidum and Cera flava She Sannang;
the viable count of the bacillus licheniformis and the brucellosis agglomerans is respectively more than 1 multiplied by 10 8 CUF/mL or the viable count of the Bacillus licheniformis and the Bullezia agglomerans respectivelyGreater than 1X 10 8 CUF/g; the number of viable bacteria of the heat-resistant streptomycete, the aspergillus broodstock, the pure trichoderma reesei, the penicillium purpurogenum, the siderophore thermophilus, the geotrichum candidum and the Eurotium cereum is respectively more than 1 multiplied by 10 6 CUF/mL or the viable count of the heat-resistant streptomyces, aspergillus broodstocus, pure trichoderma reesei, penicillium purpurogenum, siderophore thermophile, geotrichum candidum and Eurotium cereum is respectively more than 1 multiplied by 10 6 CUF/g。
The source of the microorganism of the composite microbial agent is not particularly limited, and commercially available products known to those skilled in the art can be used.
The culture medium for culturing the microorganisms is not particularly limited, and culture media which are known to those skilled in the art and are suitable for the growth of the respective microorganisms may be used.
In the present invention, the medium for culturing the Bacillus licheniformis is preferably a nutrient broth medium; the culture medium for culturing the Bullerenia collectica is preferably a nutrient gravy agar culture medium; the medium for culturing the Streptomyces thermoduricus is preferably MS medium; the culture medium for culturing the aspergillus broodstock is preferably potato liquid culture medium; the culture medium for culturing pure myceliophthora flavum is preferably CPDA liquid culture medium; the culture medium for culturing the penicillium purpurogenum is preferably PDA liquid culture medium; the culture medium for culturing the thermophilic side spore mould is preferably PDA liquid culture medium; the culture medium for culturing the geotrichum candidum is preferably PDA liquid culture medium; the culture medium for culturing the Eurotium cristatum is preferably PDB liquid culture medium.
In the present invention, when the composite microbial agent is a liquid, the composite microbial agent preferably includes, in equal parts by volume: bacillus licheniformis, bulletia glottalis, streptomyces thermodurus, aspergillus broodstocus, mucor pulmonale, penicillium purpurogenum, sporotrichum thermophilum, geotrichum candidum and Cera flava She Sannang;
when the composite microbial agent is solid, the composite microbial agent preferably comprises the following components in parts by mass: bacillus licheniformis, brevibacterium glomerata, streptomyces thermodurus, aspergillus broodstocus, mucor pulmonale, penicillium purpurogenum, sporotrichum thermophilum, geotrichum candidum and Eurotium cereum.
The invention collects typical coastal zone plant and soil samples in north of China, determines microorganisms, and performs network analysis on the combination of plant and soil microorganisms to analyze a core microbiome; the composite microbial agent obtained by compounding different microorganisms is not only beneficial to the establishment of degraded tidal flat vegetation, but also has high survival rate, can promote the accumulation of soil organic matters, and has low recovery cost.
The invention also provides a composition for ecological restoration, which comprises the composite microbial inoculum and biochar. The present invention is not particularly limited to the source of the biochar, and may be commercially available products known to those skilled in the art.
In the invention, the mass ratio of the composite microbial inoculum to the biochar is preferably (0.5-3): 100, more preferably (1 to 2): 100, more preferably 1.5:100.
the invention also provides a preparation method of the composition, which comprises the following steps: and mixing the composite microbial inoculum with biochar to obtain the composition.
In the invention, when the composite microbial inoculum is liquid, the composite microbial inoculum is preferably mixed and then dried at a temperature lower than 40 ℃ to obtain the composition; more preferably at 25-28 ℃; the mixing means preferably comprises spraying. According to the invention, through airing treatment at normal temperature (25-28 ℃), microorganisms in the composite microbial agent can be better adsorbed on biochar, and the activity of the microorganisms can be maintained.
The invention also provides application of the composite microbial inoculum or the composition prepared by the preparation method in promoting ecological restoration of degraded tidal flat.
In the present invention, the ecological restoration preferably includes promoting vegetation establishment and/or promoting soil organic matter accumulation; the promotion of vegetation establishment preferably includes increasing plant germination and promoting plant growth.
In the present invention, the area for ecological restoration preferably includes an area having a soil salt content of 3 to 8%o, and more preferably includes a northern coastal degraded beach area. The composite microbial agent provided by the invention is applied to the area to be recovered as a core microbial group, is beneficial to the establishment of degraded tidal flat vegetation, has high survival rate, can promote the accumulation of soil organic matters, and has low recovery cost.
The invention also provides a method for promoting the ecological restoration of the degraded tidal flat, which comprises the following steps: mixing the composition with soil of the area to be restored. In the present invention, the amount of the composition is preferably 1000 kg/mu.
In order to further illustrate the present invention, the following examples are provided to describe in detail a composite microbial agent for ecological restoration, a composition, a preparation method and an application thereof, but they should not be construed as limiting the scope of the present invention.
Example 1
The composite microbial inoculum for ecological restoration consists of the following components: bacillus licheniformis 10mL (viable count 1×10) 8 CUF/mL), 10mL of Klebsiella agglomerans (viable count 1X 10) 8 CUF/mL), streptomyces thermoduricus 10mL (viable count 1×10) 6 CUF/mL), aspergillus broodstock 10mL (viable count 1×10) 6 CUF/mL), 10mL of pure Chlamydia trachomatis (viable count 1X 10) 6 CUF/mL), penicillium purpurogenum 10mL (viable count 1×10) 6 CUF/mL), 10mL of a side spore mold thermophile (the viable count is 1X 10) 6 CUF/mL), geotrichum candidum 10mL (viable count 1×10) 6 CUF/mL) and 10mL of wax She Sannang bacteria (viable count 1X 10) 6 CUF/mL)；
The culture mediums for propagating the microorganisms are respectively as follows: the culture medium for culturing the bacillus licheniformis is a nutrient broth culture medium; the culture medium for culturing the Bulletia glottaria is a nutrient gravy agar culture medium; the culture medium for culturing the streptomyces thermotolerans is an MS culture medium; the culture medium for culturing the aspergillus broodstock is potato liquid culture medium; the culture medium for culturing pure myceliophthora flavum is CPDA liquid culture medium; the culture medium for culturing the penicillium purpurogenum is PDA liquid culture medium; the culture medium for culturing the thermophilic side spore mould is PDA liquid culture medium; the culture medium for culturing the geotrichum candidum is PDA liquid culture medium; the culture medium for culturing the Eurotium cristatum is PDB liquid culture medium.
Example 2
A composition for ecological restoration, consisting of the following components: 0.5g of the composite microbial inoculum prepared in the example 1 and 100g of biochar;
the preparation of the composition consists of the following steps:
spraying the composite microbial inoculum on biochar, and airing at 25-28 ℃ to obtain the composition.
Example 3
A composition for ecological restoration, consisting of the following components: 1.5g of the composite microbial inoculum prepared in example 1 and 100g of biochar.
The preparation of the composition was the same as in example 2.
Example 4
A composition for ecological restoration, consisting of the following components: 3g of the composite microbial inoculum prepared in example 1 and 100g of biochar.
The preparation of the composition was the same as in example 2.
Comparative example 1
The composite microbial inoculum for ecological restoration consists of the following components: bacillus licheniformis 10mL (viable count 1×10) 8 CUF/mL), streptomyces thermoduricus 10mL (viable count 1×10) 6 CUF/mL), aspergillus broodstock 10mL (viable count 1×10) 6 CUF/mL) and Penicillium purpurogenum 10mL (viable count 1X 10) 6 CUF/mL)；
The culture mediums for propagating the microorganisms are respectively as follows:
the culture medium for culturing the bacillus licheniformis is a nutrient broth culture medium;
the culture medium for culturing the streptomyces thermotolerans is an MS culture medium;
the culture medium for culturing the aspergillus broodstock is potato liquid culture medium;
the culture medium for culturing the penicillium purpurogenum is PDA liquid culture medium.
Comparative example 2
The composite microbial inoculum for ecological restoration consists of the following components: bacillus licheniformis 10mL (viable count 1×10) 8 CUF/mL), 10mL of Klebsiella agglomerans (viable count 1X 10) 8 CUF/mL), aspergillus broodstock 10mL (viable count 1×10) 6 CUF/mL), pure myceliophthora flavum10mL (viable count of 1X 10) 6 CUF/mL), penicillium purpurogenum 10mL (viable count 1×10) 6 CUF/mL) and 10mL of a thermophilic side spore mold (viable count 1X 10) 6 CUF/mL)；
The culture mediums for propagating the microorganisms are respectively as follows:
the culture medium for culturing the bacillus licheniformis is preferably a nutrient broth culture medium;
the culture medium for culturing the Bullerenia collectica is preferably a nutrient gravy agar culture medium;
the culture medium for culturing the aspergillus broodstock is preferably potato liquid culture medium;
the culture medium for culturing pure myceliophthora flavum is CPDA liquid culture medium;
the culture medium for culturing the penicillium purpurogenum is preferably PDA liquid culture medium;
the culture medium for culturing the thermophilic side spore mold is preferably PDA liquid culture medium.
Comparative example 3
A composition similar to example 2, except that 0.5g of the complex microbial agent prepared in example 1 was replaced with 0.5g of the medium (without microorganisms) for propagation of the above microorganisms in example 1.
Comparative example 4
A composition similar to example 2, except that 0.5g of the composite microbial agent prepared in example 1 was replaced with 0.5g of the composite microbial agent prepared in comparative example 1.
Comparative example 5
A composition similar to example 2, except that 0.5g of the composite microbial agent prepared in example 1 was replaced with 0.5g of the composite microbial agent prepared in comparative example 2.
Application example 1
A method for promoting ecological restoration of degraded tidal flat, which comprises the following steps:
the composition prepared in example 2 was used for soil preparation at a dose of 1000 kg/mu, sesbania was sown after soil preparation, the sowing density was 12000 plants/mu, and seedling was carried out according to the normal procedure.
Application example 2
A method for promoting restoration of degraded tidal flat ecology similar to application example 1, the only difference being that the composition prepared in example 2 is replaced with the same amount of the composition prepared in example 3.
Application example 3
A method for promoting restoration of degraded tidal flat ecology similar to application example 1, the only difference being that the composition prepared in example 2 is replaced with the same amount of the composition prepared in example 4.
Comparative application example 1
A method for promoting restoration of degraded tidal flat ecology similar to that of application example 1, the only difference being that the composition prepared in example 2 was replaced with the composition prepared in comparative example 3 in equal amount.
Comparative application example 2
A method for promoting restoration of degraded tidal flat ecology similar to that of application example 1, the only difference being that the composition prepared in example 2 was replaced with the composition prepared in comparative example 4 in equal amount.
Comparative application example 3
A method for promoting restoration of degraded tidal flat ecology similar to application example 1, the only difference being that the composition prepared in example 2 was replaced with the composition prepared in comparative example 5 in equal amount.
Application example 4
The germination rate (germination rate=germination number/sowing number×100%) at 2 weeks after sowing and the dry weight of the above-ground biomass 180d after sowing were calculated for sesbania sown in application examples 1 to 3 and comparative application examples 1 to 3, respectively, wherein the test site was a farm tested in the coastal region of Jiangsu salt city, the salt content was 5%o, the soil preparation time was the beginning of 2020 month 4, and the results are shown in table 1.
TABLE 1 effects of application of different compositions on Sesbania germination and growth
| Germination percentage (%) | Aboveground biomass (g/plant) | |
| Application example 1 | 100％ | 20.5 |
| Application example 2 | 95％ | 19.8 |
| Application example 3 | 96％ | 20.3 |
| Comparative application example 1 | 85％ | 12.8 |
| Comparative application example 2 | 82％ | 11.2 |
| Comparative application example 3 | 90％ | 13.5 |
As shown in Table 1, compared with the comparative application example 1 group without the composite microbial inoculum, the germination rate of the composite microbial inoculum provided by the invention is improved by 11.8-17.6%, and the above-ground biomass is improved by 54.7-60.2%; compared with comparative application examples 1 and 2 of other composite microbial agents, the germination rate of the composite microbial agent provided by the invention is improved by 5.6% -22.0%, and the above-ground biomass is improved by 46.7% -83.0%; the composite microbial inoculum provided by the invention can obviously promote vegetation establishment of degraded tidal flat.
Application example 5
Seeding application example 4 with different groups of sesbania (application examples 1-3 and comparative application examples 1-3), respectively naturally decomposing in situ after the sesbania is apoptotic in autumn and winter each year, not performing manual intervention, continuously measuring the organic matter content of soil (potassium dichromate capacity method-external heating method) and the composition of aggregate particles in 3 years, weighing 100g of air-dried undisturbed soil sample during wet sieving, firstly placing on a 2mm sieve and soaking for 10min, and then performing wet sieving with a granule analyzer, wherein the grading frequency is 1350r/min, and the grading time is 5min; after wet sieving, the agglomerates of different size fractions were collected and dried at 50 ℃, referred to as mass. The percentage of soil aggregate is calculated by dividing the mass of the large and small particles by the total mass, and the measurement results are shown in Table 2.
TABLE 2 Effect of application of slightly different compositions on soil organic matter content
Note that: the large particle aggregates and the small particle aggregates are distinguished by a size fraction of 0.25mm, the number of large aggregates with soil >0.25mm is positively correlated with the soil condition, and the higher the content is, the better the soil stability is.
As shown in Table 2, the composite microbial inoculum provided by the invention can obviously promote the accumulation of soil organic matters and has better soil stability compared with comparative application examples 1-3.
In conclusion, the composite microbial agent obtained by compounding different microorganisms is beneficial to the establishment of degraded tidal flat vegetation, has high germination rate, can promote the accumulation of soil organic matters, and has low recovery cost.
While the invention has been described in terms of preferred embodiments, it is not intended to be limited thereto, but rather to enable any person skilled in the art to make various changes and modifications without departing from the spirit and scope of the present invention, which is therefore to be limited only by the appended claims.
Claims (10)
1. By usingThe composite microbial inoculum for ecological restoration is characterized by comprising the following components: bacillus licheniformis [ ]Bacillus licheniformis) Bullerenz agglomeransLabrenzia aggregata) Heat-resistant streptomyceteStreptomyces thermotolerans) Aspergillus broomAspergillus penicillioides) Rhizopus luteus (L.) SprengByssochlamys fulva) Penicillium purpurogenum (L.) HsiaoPenicillium purpurogenum) Thermophilic side spore mouldSporotrichum thermophile) Radix rehmanniae Preparata, geotrichum candidumGeotrichum candidum) And wax She Sannang bacteria%Eurotium herbariorum）；
The number of viable bacteria of the bacillus licheniformis and the brucellosis agglomerans is larger than 1 multiplied by 10 8 CFU/mL or 1X 10 8 CFU/g; the number of viable bacteria of the heat-resistant streptomycete, the aspergillus broodstock, the pure trichoderma reesei, the penicillium purpurogenum, the siderophore thermophilus, the geotrichum candidum and the Eurotium cereum is larger than 1 multiplied by 10 6 CFU/mL or 1X 10 6 CFU/g。
2. The composite microbial agent of claim 1, wherein when the composite microbial agent is a liquid, the composite microbial agent is an equal volume fraction: bacillus licheniformis, bulletia glottalis, streptomyces thermodurus, aspergillus broodstocus, mucor pulmonale, penicillium purpurogenum, sporotrichum thermophilum, geotrichum candidum and Cera flava She Sannang;
when the composite microbial inoculum is solid, the composite microbial inoculum is prepared from the following components in parts by mass: bacillus licheniformis, brevibacterium glomerata, streptomyces thermodurus, aspergillus broodstocus, mucor pulmonale, penicillium purpurogenum, sporotrichum thermophilum, geotrichum candidum and Eurotium cereum.
3. A composition for ecological restoration, characterized in that it consists of the complex microbial agent of claim 1 or 2 and biochar.
4. The composition of claim 3, wherein the mass ratio of the composite microbial agent to the biochar is (0.5-3): 100.
5. a process for preparing a composition according to claim 3 or 4, comprising: mixing the composite microbial agent of claim 1 or 2 with biochar to obtain the composition.
6. The method of claim 5, wherein when the composite microbial agent is a liquid, the method further comprises air drying at a temperature below 40 ℃ to obtain the composition.
7. Application of the composite microbial inoculum of claim 1 or 2 or the composition of claim 3 or 4 or the composition prepared by the preparation method of claim 5 or 6 in promoting ecological restoration of degraded tidal flat; the ecological restoration is for promoting vegetation establishment and/or promoting soil organic matter accumulation; the promotion of vegetation is established to increase the germination rate of plants and promote plant growth.
8. The use according to claim 7, wherein the areas of ecological restoration comprise areas with a soil salt content of 3-8%.
9. The use according to claim 8, wherein the area of ecological restoration comprises an area with 5%o salt content in the soil.
10. A method for promoting ecological restoration of a degraded tidal flat, comprising: mixing the composition of claim 3 or 4 with soil in the area to be restored; the ecological restoration is for promoting vegetation establishment and/or promoting soil organic matter accumulation; the promotion of vegetation is established to increase the germination rate of plants and promote plant growth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210447769.2A CN114606168B (en) | 2022-04-26 | 2022-04-26 | Composite microbial inoculum and composition for ecological restoration, and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210447769.2A CN114606168B (en) | 2022-04-26 | 2022-04-26 | Composite microbial inoculum and composition for ecological restoration, and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114606168A CN114606168A (en) | 2022-06-10 |
| CN114606168B true CN114606168B (en) | 2023-12-22 |
Family
ID=81868879
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210447769.2A Active CN114606168B (en) | 2022-04-26 | 2022-04-26 | Composite microbial inoculum and composition for ecological restoration, and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114606168B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109679860A (en) * | 2018-11-30 | 2019-04-26 | 北京国环清华环境工程设计研究院有限公司 | A kind of composite bacteria agent and the preparation method and application thereof for the processing of gardens green waste |
| CN110373207A (en) * | 2019-07-29 | 2019-10-25 | 苏州沣懿生物科技有限公司 | A kind of microbial bacterial agent and its preparation process of alkaline land improving |
| CN113388528A (en) * | 2021-06-15 | 2021-09-14 | 中国林业科学研究院林业研究所 | Ground surface sacculus mould, composition for saline-alkali soil remediation and saline-alkali soil remediation method |
-
2022
- 2022-04-26 CN CN202210447769.2A patent/CN114606168B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109679860A (en) * | 2018-11-30 | 2019-04-26 | 北京国环清华环境工程设计研究院有限公司 | A kind of composite bacteria agent and the preparation method and application thereof for the processing of gardens green waste |
| CN110373207A (en) * | 2019-07-29 | 2019-10-25 | 苏州沣懿生物科技有限公司 | A kind of microbial bacterial agent and its preparation process of alkaline land improving |
| CN113388528A (en) * | 2021-06-15 | 2021-09-14 | 中国林业科学研究院林业研究所 | Ground surface sacculus mould, composition for saline-alkali soil remediation and saline-alkali soil remediation method |
Non-Patent Citations (2)
| Title |
|---|
| 散囊菌产次级代谢物及其生物活性研究进展;许爱清;;食品工业科技(第01期);第330-335页 * |
| 海洋二甲基硫生态模型研究综述;赵亮;沈家葳;蒋昊;;中国海洋大学学报(自然科学版)(第11期);第4-13页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114606168A (en) | 2022-06-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Mishra et al. | Characterisation of a psychrotolerant plant growth promoting Pseudomonas sp. strain PGERs17 (MTCC 9000) isolated from North Western Indian Himalayas | |
| CN104450597B (en) | Preparation method of petroleum degrading bacteria solid microbial inoculum and method for repairing petroleum-polluted soil by using solid microbial inoculum prepared by preparation method | |
| CN106754515B (en) | Screening method of growth promoting strains for improving salt tolerance of crops | |
| CN109370955B (en) | Bacillus vallismortis HSB-2 and application thereof | |
| CN109652329A (en) | A kind of preparation and application of bacillus solid microbial | |
| CN105925503B (en) | One plant of salt tolerant rhizosphere growth-promoting enterobacter cloacae and its application | |
| CN114196591B (en) | Pseudomonas flavescens strain KY592 with passivation effect on heavy metal cadmium and application thereof | |
| CN106916756B (en) | Fungus strain, microbial agent, straw soil remediation agent and application thereof | |
| CN113549570B (en) | Soil antagonistic bacterium preparation of momordica grosvenori soil-borne pathogenic bacteria, microbial fertilizer and application | |
| CN102604869B (en) | Bio-control bacteria strain 1JN2 for preventing and treating bacterial fruit blotches of watermelons and application thereof | |
| CN114606168B (en) | Composite microbial inoculum and composition for ecological restoration, and preparation method and application thereof | |
| CN111117909B (en) | Strain capable of resisting multiple heavy metals and promoting plant growth and application thereof | |
| CN106282052B (en) | It is a kind of improve coastal saline soil compound lactobacillus preparation and its application | |
| CN111100809B (en) | Bacillus mojavensis for preventing and treating root rot of cotton at seedling stage and application thereof | |
| CN104805035A (en) | Rhodococcus sp. 2G used for simultaneous degradation of plurality of phthalic acid esters | |
| CN114934000B (en) | Salt-tolerant bacillus amyloliquefaciens and screening and application thereof | |
| CN110684695A (en) | Paenibacillus polymyxa QZY-1 and application thereof | |
| CN105274031A (en) | Sphingomonas sp. and its application | |
| CN106818442A (en) | A kind of wheat straw type tobacco moistening seedling medium and preparation method thereof | |
| CN114836348A (en) | Microbial agent for improving soil salinization and application thereof | |
| CN113637616A (en) | Salt-tolerant growth-promoting microbacterium and application thereof as well as live bacterium preparation based on salt-tolerant growth-promoting microbacterium | |
| CN112779187A (en) | Bacillus quinquefolius and application thereof | |
| CN106929444B (en) | Bacillus and application thereof | |
| CN111117640A (en) | Saline-alkali soil improver containing glomus mosseae and improvement method | |
| CN111117920A (en) | Bacillus mycoides producing protease and siderophore and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |