CN114606168A - Complex microbial inoculant and composition for ecological restoration, and preparation method and application thereof - Google Patents
Complex microbial inoculant and composition for ecological restoration, and preparation method and application thereof Download PDFInfo
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 19
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 239000002054 inoculum Substances 0.000 title claims description 11
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 53
- 239000002689 soil Substances 0.000 claims abstract description 35
- 238000000034 method Methods 0.000 claims abstract description 15
- 238000009825 accumulation Methods 0.000 claims abstract description 10
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 6
- 241000194108 Bacillus licheniformis Species 0.000 claims description 25
- 241001540751 Talaromyces ruber Species 0.000 claims description 24
- 235000017388 Geotrichum candidum Nutrition 0.000 claims description 20
- 241001600136 Streptomyces thermotolerans Species 0.000 claims description 20
- 230000001737 promoting effect Effects 0.000 claims description 20
- 241000132177 Aspergillus glaucus Species 0.000 claims description 11
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- 239000004016 soil organic matter Substances 0.000 claims description 7
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- 238000002156 mixing Methods 0.000 claims description 6
- 241000223257 Thermomyces Species 0.000 claims description 5
- 241001361634 Rhizoctonia Species 0.000 claims description 4
- 241000134912 Aspergillus penicillioides Species 0.000 claims description 3
- 241000123346 Chrysosporium Species 0.000 claims description 3
- 241001116661 Labrenzia aggregata Species 0.000 claims description 3
- 241000187747 Streptomyces Species 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 241000131314 Aspergillus candidus Species 0.000 claims description 2
- 241000232299 Ralstonia Species 0.000 claims description 2
- 241000453701 Galactomyces candidum Species 0.000 claims 5
- 238000004519 manufacturing process Methods 0.000 claims 1
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- 238000009331 sowing Methods 0.000 description 5
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- GISJHCLTIVIGLX-UHFFFAOYSA-N n-[4-[(4-chlorophenyl)methoxy]pyridin-2-yl]-2-(2,6-difluorophenyl)acetamide Chemical compound FC1=CC=CC(F)=C1CC(=O)NC1=CC(OCC=2C=CC(Cl)=CC=2)=CC=N1 GISJHCLTIVIGLX-UHFFFAOYSA-N 0.000 description 3
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention relates to the technical field of ecological restoration, in particular to a composite microbial inoculum and a composition for ecological restoration, and a preparation method and application thereof. According to the method, typical coastal zone plants and soil samples in northern China are collected, microorganisms are measured, and the microorganisms of the plants and the soil are combined to perform network analysis, so that a core microorganism group is analyzed; the compound microbial agent obtained by compounding different microorganisms is beneficial to establishment of degraded beach vegetation, has high germination rate, can promote accumulation of soil organic matters, and has low recovery cost. According to the embodiment of the invention, compared with a control group not using the compound microbial inoculum, the germination rate of a test group using the compound microbial inoculum is improved by 17.6%, and the organic matter content of soil is improved by 41.5%.
Description
Technical Field
The invention relates to the technical field of ecological restoration, in particular to a composite microbial inoculum and a composition for ecological restoration, and a preparation method and application thereof.
Background
Besides the salt and alkali stress, environmental pollution and artificial activity interference which hinder plant growth and vegetation establishment, the fact that soil organic matters are easy to lose and difficult to accumulate also is a key problem which hinders ecological restoration. The vegetation is difficult to establish and the soil organic matter is difficult to accumulate, the vegetation and the soil organic matter are influenced mutually, namely, the vegetation establishment is hindered due to low soil organic matter content, nutrients cannot be returned to the soil if the vegetation cannot be established, and the soil organic matter content cannot be increased. The traditional vegetation recovery usually takes direct afforestation as a main measure, the main measures comprise soil dressing, organic fertilizer application, fixation and the like, the afforestation cost is higher, and the germination rate is low.
Disclosure of Invention
In order to solve the problems, the invention provides a complex microbial inoculum for ecological restoration, a composition, a preparation method and application thereof. The composite microbial inoculum provided by the invention is beneficial to establishment of degraded beach vegetation, has high germination rate, can promote accumulation of soil organic matters, and has low recovery cost.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides a complex microbial inoculum for ecological restoration, which comprises the following components: bacillus licheniformis (Bacillus licheniformis), Ralstonia agglomerans (Labrenzia aggregata), Streptomyces thermotolerans (Streptomyces thermolerans), Aspergillus candidus (Aspergillus penicillioides), Chlamydomonas chrysosporium fulva, Penicillium purpurogenum (Penicillium purpurogenum), Thermomyces thermophilus (Sporotrichum thermophile), Geotrichum candidum (Geotrichum candidum) and Eurotium cerotium (Eurotium candidum);
the viable count of the bacillus licheniformis and the Larreezia agglomerans is respectively more than 1 multiplied by 108CUF/mL or 1X 108CUF/g; the viable count of the streptomyces thermotolerans, the aspergillus glaucus, the pure rhizoctonia flavus, the penicillium purpurogenum, the sporotrichum thermophilum, the geotrichum candidum and the eurotium cristatum is respectively more than 1 multiplied by 106CUF/mL or 1X 106CUF/g。
Preferably, when the complex microbial inoculant is a liquid, the complex microbial inoculant comprises the following components in parts by volume: bacillus licheniformis, Larrez agglomerans, streptomyces thermotolerans, Aspergillus glaucus, Chlamydomonas flavivis, Penicillium purpurogenum, Byssochlamus thermophilus, Geotrichum candidum and Ascomyces cereus;
when the complex microbial inoculum is solid, the complex microbial inoculum comprises the following components in parts by mass: bacillus licheniformis, Larrez agglomerans, Streptomyces thermotolerans, Aspergillus kawachii, Chlamydia chrysogenum, Penicillium purpurogenum, Pleurotus thermophilus, Geotrichum candidum and Ascomyces cerealis.
The invention also provides a composition for ecological restoration, which comprises the composite microbial inoculum and biochar.
Preferably, the mass ratio of the composite microbial inoculum to the biochar is (0.5-3): 100.
the invention also provides a preparation method of the composition, which comprises the following steps: and mixing the composite microbial inoculum with the biochar to obtain the composition.
Preferably, when the complex microbial inoculum is liquid, the complex microbial inoculum is dried at the temperature lower than 40 ℃ after being mixed to obtain the composition.
The invention also provides the application of the composite microbial inoculum or the composition prepared by the preparation method in promoting ecological restoration of degraded mudflat.
Preferably, the ecological restoration comprises promoting vegetation establishment and/or promoting soil organic matter accumulation.
Preferably, the area for ecological restoration comprises an area with the soil salt content of 3-8 per mill.
The invention also provides a method for promoting ecological restoration of the degraded tidal flat, which comprises the following steps: the above composition is mixed with the soil of the area to be rehabilitated.
Has the advantages that:
the invention provides a complex microbial inoculum for ecological restoration, which comprises the following components: bacillus licheniformis (Bacillus licheniformis), Brevibacterium agglomerans (Labrenzia aggregata), Streptomyces thermotolerans (Streptomyces thermoLERANs), Aspergillus furiosus (Aspergillus penicillioides), Chlamydomonas chrysosporium fulva, Penicillium purpurogenum (Penicillium thermophilum), Therosporium candidum (Geotrichum candidum) and Eurotium spicatum (Eurotium barorhizorum); the viable count of the bacillus licheniformis and the Larreezia agglomerans is respectively more than 1 multiplied by 108CUF/mL or 1X 108CUF/g; the viable count of the streptomyces thermotolerans, the aspergillus glaucus, the pure rhizoctonia flavus, the penicillium purpurogenum, the sporotrichum thermophilum, the geotrichum candidum and the eurotium cristatum is respectively more than 1 multiplied by 106CUF/mL or 1X 106CUF/g. According to the method, typical coastal zone plants and soil samples in northern China are collected, microorganisms are measured, and the microorganisms of the plants and the soil are combined to perform network analysis, so that a core microorganism group is analyzed; the compound microbial agent obtained by compounding different microorganisms is beneficial to the establishment of degraded beach vegetation, has high germination rate, can promote the accumulation of soil organic matters, and has low recovery cost. As can be seen from the examples of the present invention, the test group using the complex microbial inoculum was compared to the test group not using the complex microbial inoculumThe germination rate of the control group of the microbial inoculum is improved by 17.6 percent, and the organic matter content of the soil is improved by 41.5 percent.
Detailed Description
The invention provides a complex microbial inoculum for ecological restoration, which comprises the following components: bacillus licheniformis, Larrez agglomerans, streptomyces thermotolerans, Aspergillus glaucus, Chlamydomonas flavivis, Penicillium purpurogenum, Byssochlamus thermophilus, Geotrichum candidum and Ascomyces cereus;
the viable count of the bacillus licheniformis and the Larreezia agglomerans is respectively more than 1 multiplied by 108The number of the viable bacteria of the CUF/mL or the Bacillus licheniformis and the Larrezia agglomerans is respectively more than 1 × 108CUF/g; the viable count of the streptomyces thermotolerans, the aspergillus glaucus, the pure rhizoctonia flavus, the penicillium purpurogenum, the sporotrichum thermophilum, the geotrichum candidum and the eurotium cristatum is respectively more than 1 multiplied by 106CUF/mL or viable count of the Streptomyces thermotolerans, Aspergillus glaucus, Chlamydomonas flaviviridis, Penicillium purpurogenum, Thermomyces thermophilus, Geotrichum candidum and Eurotium cristatum are respectively more than 1 × 106CUF/g。
The source of the complex microbial inoculum of the invention is not particularly required, and commercially available products known to those skilled in the art can be used.
The medium for culturing the above microorganisms is not particularly limited in the present invention, and a medium suitable for growth of the respective microorganisms known to those skilled in the art may be used.
In the present invention, the medium for culturing the bacillus licheniformis is preferably a nutrient broth medium; the culture medium for culturing the Laurette agglomerans is preferably a nutrient gravy agar culture medium; the culture medium for culturing the streptomyces thermotolerans is preferably an MS culture medium; the culture medium for culturing aspergillus kawachii is preferably a potato liquid culture medium; the culture medium for culturing pure rhizopus flavus is preferably CPDA liquid culture medium; the culture medium for culturing the penicillium purpurogenum is preferably a PDA liquid culture medium; the culture medium for culturing the thermophilic sporotrichum is preferably PDA liquid culture medium; the culture medium for culturing the geotrichum candidum is preferably a PDA liquid culture medium; the culture medium for culturing the eurotium cristatum is preferably a PDB liquid culture medium.
In the present invention, when the complex microbial inoculum is a liquid, the complex microbial inoculum preferably comprises equal parts by volume of: bacillus licheniformis, Larrez agglomerans, streptomyces thermotolerans, Aspergillus glaucus, Chlamydomonas flavivis, Penicillium purpurogenum, Byssochlamus thermophilus, Geotrichum candidum and Ascomyces cereus;
when the complex microbial inoculum is a solid, the complex microbial inoculum preferably comprises the following components in parts by mass: bacillus licheniformis, Larrez agglomerans, streptomyces thermotolerans, Aspergillus glaucus, Chlamydomonas flavivis, Penicillium purpurogenum, Byssochlamus thermophilus, Geotrichum candidum and Ascomyces cereus.
According to the method, typical coastal zone plants and soil samples in northern China are collected, microorganisms are measured, and the microorganisms of the plants and the soil are combined to perform network analysis, so that a core microorganism group is analyzed; the compound microbial agent obtained by compounding different microorganisms is beneficial to establishment of degraded beach vegetation, has high survival rate, can promote accumulation of soil organic matters, and has low recovery cost.
The invention also provides a composition for ecological restoration, which comprises the composite microbial inoculum and biochar. The source of the biochar is not particularly required in the invention, and commercially available products well known to those skilled in the art can be adopted.
In the invention, the mass ratio of the complex microbial inoculum to the biochar is preferably (0.5-3): 100, more preferably (1-2): 100, more preferably 1.5: 100.
the invention also provides a preparation method of the composition, which comprises the following steps: and mixing the composite microbial inoculum with the biochar to obtain the composition.
In the invention, when the complex microbial inoculum is liquid, the method preferably further comprises the step of airing at the temperature lower than 40 ℃ after mixing to obtain the composition; more preferably, the air drying is carried out at the temperature of 25-28 ℃; the means of mixing preferably comprises spraying. The invention can lead the microorganism in the composite microbial inoculum to be better adsorbed on the biochar and maintain the activity of the microorganism by airing at normal temperature (25-28 ℃).
The invention also provides the application of the composite microbial inoculum or the composition prepared by the preparation method in promoting ecological restoration of degraded mudflat.
In the present invention, the ecological restoration preferably comprises promoting vegetation establishment and/or promoting soil organic matter accumulation; the promoting vegetation establishment preferably includes increasing plant germination and promoting plant growth.
In the present invention, the area for ecological restoration preferably includes an area with a soil salt content of 3 to 8 per mill, and more preferably includes a northern coastal degenerated tidal flat area. The composite microbial agent provided by the invention is used as a core microbial group to be applied to an area to be restored, so that the establishment of degraded beach vegetation is facilitated, the survival rate is high, the accumulation of organic matters in soil can be promoted, and the restoration cost is low.
The invention also provides a method for promoting ecological restoration of the degraded tidal flat, which comprises the following steps: the above composition is mixed with the soil of the area to be rehabilitated. In the present invention, the amount of the composition is preferably 1000 kg/acre.
In order to further illustrate the present invention, the complex microbial inoculum, the composition, the preparation method and the application thereof for ecological restoration provided by the present invention are described in detail below with reference to the examples, but they should not be construed as limiting the scope of the present invention.
Example 1
A composite microbial inoculum for ecological restoration comprises the following components: bacillus licheniformis 10mL (viable count is 1 × 10)8CUF/mL), Larrez agglomerans 10mL (viable count 1X 10)8CUF/mL), Streptomyces thermotolerans 10mL (viable count is 1X 10)6CUF/mL), 10mL of Aspergillus broom (viable count 1X 10)6CUF/mL), 10mL of pure phoma flavum (viable count 1X 10)6CUF/mL), Penicillium purpurogenum 10mL (viable count 1X 10)6CUF/mL), 10mL of Thermomyces thermophilus (viable count 1X 10)6CUF/mL), Geotrichum candidum 10mL (viable count is 1X 10)6CUF/mL) and 10mL of Eurotium cristatum (viable count of 1X 10)6CUF/mL)；
The culture mediums for propagating the microorganisms are respectively as follows: the culture medium for culturing the bacillus licheniformis is a nutrient broth culture medium; the culture medium for culturing the Laurette agglomerans is a nutrient gravy agar culture medium; the culture medium for culturing the streptomyces thermotolerans is an MS culture medium; the culture medium for culturing aspergillus kawachii is a potato liquid culture medium; the culture medium for culturing pure rhizopus flavus is CPDA liquid culture medium; the culture medium for culturing the penicillium purpurogenum is a PDA liquid culture medium; the culture medium for culturing the sporotrichum thermophilum is a PDA liquid culture medium; the culture medium for culturing the geotrichum candidum is a PDA liquid culture medium; the culture medium for culturing the eurotium cristatum is a PDB liquid culture medium.
Example 2
A composition for ecological restoration, consisting of the following components: 0.5g of the composite microbial inoculum prepared in the example 1 and 100g of biochar;
the composition was prepared from the following:
and spraying the complex microbial inoculum on charcoal, and airing at 25-28 ℃ to obtain the composition.
Example 3
A composition for ecological restoration, consisting of the following components: 1.5g of the composite microbial inoculum prepared in the example 1 and 100g of biochar.
The composition was prepared as in example 2.
Example 4
A composition for ecological restoration, consisting of the following components: 3g of the composite microbial inoculum prepared in the example 1 and 100g of biochar.
The composition was prepared as in example 2.
Comparative example 1
A composite microbial inoculum for ecological restoration comprises the following components: bacillus licheniformis 10mL (viable count is 1 × 10)8CUF/mL), Streptomyces thermotolerans 10mL (viable count is 1X 10)6CUF/mL), 10mL of Aspergillus broom (viable count 1X 10)6CUF/mL) and 10mL of Penicillium purpurogenum (viable count 1X 10)6CUF/mL)；
The culture mediums for propagating the microorganisms are respectively as follows:
the culture medium for culturing the bacillus licheniformis is a nutrient broth culture medium;
the culture medium for culturing the streptomyces thermotolerans is an MS culture medium;
the culture medium for culturing aspergillus kawachii is a potato liquid culture medium;
the culture medium for culturing the penicillium purpurogenum is PDA liquid culture medium.
Comparative example 2
A composite microbial inoculum for ecological restoration comprises the following components: bacillus licheniformis 10mL (viable count is 1X 10)8CUF/mL), Larrez agglomerans 10mL (viable count 1X 10)8CUF/mL), 10mL of Aspergillus broom (viable count 1X 10)6CUF/mL), 10mL of pure myceliophthora flava (viable count 1X 10)6CUF/mL), Penicillium purpurogenum 10mL (viable count 1X 10)6CUF/mL) and 10mL of Thermomyces thermophilus (viable count 1X 10)6CUF/mL)；
The culture mediums for propagating the microorganisms are respectively as follows:
the culture medium for culturing the bacillus licheniformis is preferably a nutrient broth culture medium;
the culture medium for culturing the Laurette agglomerans is preferably a nutrient gravy agar culture medium;
the culture medium for culturing aspergillus kawachii is preferably a potato liquid culture medium;
the culture medium for culturing pure rhizopus flavus is CPDA liquid culture medium;
the culture medium for culturing the penicillium purpurogenum is preferably a PDA liquid culture medium;
the culture medium for culturing the thermophilic sporotrichum is preferably PDA liquid culture medium.
Comparative example 3
A composition similar to that of example 2, except that 0.5g of the complex microbial inoculum prepared in example 1 was replaced with 0.5g of the medium (containing no microorganisms) for expanding the above microorganisms in example 1.
Comparative example 4
A composition similar to that of example 2, except that 0.5g of the complex microbial inoculum prepared in example 1 was replaced with 0.5g of the complex microbial inoculum prepared in comparative example 1.
Comparative example 5
A composition similar to that of example 2, except that 0.5g of the complex microbial inoculum prepared in example 1 was replaced with 0.5g of the complex microbial inoculum prepared in comparative example 2.
Application example 1
A method for promoting ecological restoration of a degraded mud flat comprises the following steps:
the composition prepared in example 2 was used for soil preparation at an amount of 1000 kg/mu, sesbania was sown after soil preparation at a sowing density of 12000 plants/mu, and seedlings were grown according to the normal procedure.
Application example 2
A method for promoting ecological restoration of a degraded intertidal zone similar to application example 1, except that the composition prepared in example 2 was replaced with the same amount of the composition prepared in example 3.
Application example 3
A method for promoting ecological restoration of a degraded intertidal zone similar to application example 1, except that the composition prepared in example 2 was replaced with the same amount of the composition prepared in example 4.
Comparative application example 1
A method for promoting ecological restoration of a degraded intertidal zone similar to application example 1, except that the composition prepared in example 2 was replaced with the same amount of the composition prepared in comparative example 3.
Comparative application example 2
A method for promoting ecological restoration of a degraded intertidal zone similar to application example 1, except that the composition prepared in example 2 was replaced with the same amount of the composition prepared in comparative example 4.
Comparative application example 3
A method for promoting ecological restoration of a degraded intertidal zone similar to application example 1, except that the composition prepared in example 2 was replaced with the same amount of the composition prepared in comparative example 5.
Application example 4
For sesbania sowed in application examples 1 to 3 and comparative application examples 1 to 3, the germination rate (germination rate ═ germination number/sowing number × 100%) at 2 weeks after sowing and the dry weight of biomass on the ground at 180d after sowing were calculated, respectively, wherein the test site was a test farm in an agrariaceae in coastal areas of Jiangsu Yangcheng, the salt content was 5 ‰, and the soil preparation time was at the beginning of 4 months in 2020 years, and the results are shown in Table 1.
TABLE 1 Effect of different compositions on Sesbania Germination and growth
| Percentage of germination (%) | Aboveground biomass (g/plant) | |
| Application example 1 | 100％ | 20.5 |
| Application example 2 | 95％ | 19.8 |
| Application example 3 | 96％ | 20.3 |
| Comparative application example 1 | 85％ | 12.8 |
| Comparative application example 2 | 82％ | 11.2 |
| Comparative application example 3 | 90％ | 13.5 |
As can be seen from Table 1, compared with the group 1 of comparative application without the compound microbial inoculum, the germination percentage of the compound microbial inoculum provided by the invention is improved by 11.8-17.6%, and the aboveground biomass is improved by 54.7-60.2%; compared with other composite microbial inoculants in comparative application examples 1 and 2, the germination rate of the composite microbial inoculants is improved by 5.6-22.0%, and the aboveground biomass is improved by 46.7-83.0%; therefore, the composite microbial inoculum provided by the invention obviously promotes the vegetation establishment of the degraded mudflat.
Application example 5
Sowing different groups of sesbania (application examples 1-3 and comparative application examples 1-3) corresponding to example 4, respectively and naturally decomposing the sesbania in situ after apoptosis in autumn and winter every year without manual intervention, continuously measuring the content of organic matters in soil (potassium dichromate volumetric method-external heating method) and the composition of aggregate particles in 3 years, weighing 100g of air-dried undisturbed soil sample during wet sieving, firstly placing the soil sample on a 2mm sieve and soaking for 10min, and then carrying out wet sieving classification by a granule analyzer, wherein the classification frequency is 1350r/min, and the classification time is 5 min; after wet screening, aggregates of different size fractions are collected and dried at 50 ℃ to obtain the aggregate with the weight. The percentage of soil aggregates was calculated by dividing the mass of the particles by the total mass, and the results are shown in Table 2.
TABLE 2 Effect of application of slightly different compositions on organic matter content of soil
Note: large particle aggregates and small particle aggregates are distinguished by a size fraction of 0.25mm, the number of large aggregates with soil >0.25mm is positively correlated with the soil condition, and the higher the content is, the better the soil stability is.
As can be seen from Table 2, compared with comparative application examples 1-3, the complex microbial inoculum provided by the invention can obviously promote the accumulation of organic matters in soil, and has better soil stability.
In conclusion, the compound microbial agent obtained by compounding different microorganisms is beneficial to establishment of degraded beach vegetation, has high germination rate, can promote accumulation of soil organic matters, and has low recovery cost.
Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes and modifications can be made therein by those skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (10)
1. A complex microbial inoculant for ecological restoration, which comprises: bacillus licheniformis (Bacillus licheniformis), Ralstonia agglomerans (Labrenzia aggregata), Streptomyces thermotolerans (Streptomyces thermolerans), Aspergillus candidus (Aspergillus penicillioides), Chlamydomonas chrysosporium fulva, Penicillium purpurogenum (Penicillium purpurogenum), Thermomyces thermophilus (Sporotrichum thermophile), Geotrichum candidum (Geotrichum candidum) and Eurotium cerotium (Eurotium candidum);
the viable count of the bacillus licheniformis and the Larreezia agglomerans is respectively more than 1 multiplied by 108CUF/mL or 1X 108CUF/g; the viable count of the streptomyces thermotolerans, the aspergillus glaucus, the pure rhizoctonia flavus, the penicillium purpurogenum, the sporotrichum thermophilum, the geotrichum candidum and the eurotium cristatum is respectively more than 1 multiplied by 106CUF/mL or 1X 106CUF/g。
2. The complex microbial inoculant according to claim 1, wherein when the complex microbial inoculant is a liquid, the complex microbial inoculant comprises the following components in parts by volume: bacillus licheniformis, Larrez agglomerans, streptomyces thermotolerans, Aspergillus glaucus, Chlamydomonas flavivis, Penicillium purpurogenum, Byssochlamus thermophilus, Geotrichum candidum and Ascomyces cereus;
when the complex microbial inoculum is solid, the complex microbial inoculum comprises the following components in parts by mass: bacillus licheniformis, Larrez agglomerans, streptomyces thermotolerans, Aspergillus glaucus, Chlamydomonas flavivis, Penicillium purpurogenum, Byssochlamus thermophilus, Geotrichum candidum and Ascomyces cereus.
3. A composition for ecological restoration, which comprises the complex microbial inoculant of claim 1 or 2 and biochar.
4. The composition according to claim 3, wherein the mass ratio of the complex microbial inoculum to the biochar is (0.5-3): 100.
5. a process for preparing a composition according to claim 3 or 4, comprising: mixing the complex microbial inoculum of claim 1 or 2 with biochar to obtain the composition.
6. The preparation method of claim 5, wherein when the complex microbial inoculum is liquid, the complex microbial inoculum is further dried at a temperature of less than 40 ℃ after being mixed to obtain the composition.
7. Use of the complex microbial agent of claim 1 or 2 or the composition of claim 3 or 4 or the composition prepared by the preparation method of claim 5 or 6 for promoting ecological restoration of degraded mudflat.
8. The use of claim 7, wherein the ecological restoration comprises promoting vegetation establishment and/or promoting soil organic matter accumulation.
9. The use according to claim 7 or 8, characterized in that the areas of ecological restoration comprise areas with a soil salinity of between 3 and 8% o.
10. A method for promoting ecological restoration of a degraded beach, which is characterized by comprising the following steps: mixing the composition of claim 3 or 4 with soil in an area to be rehabilitated.
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