CN111100809B - Bacillus mojavensis for preventing and treating root rot of cotton at seedling stage and application thereof - Google Patents
Bacillus mojavensis for preventing and treating root rot of cotton at seedling stage and application thereof Download PDFInfo
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- CN111100809B CN111100809B CN201911304928.8A CN201911304928A CN111100809B CN 111100809 B CN111100809 B CN 111100809B CN 201911304928 A CN201911304928 A CN 201911304928A CN 111100809 B CN111100809 B CN 111100809B
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
- A01N25/14—Powders or granules wettable
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12R2001/07—Bacillus
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses bacillus mojavensis for preventing and treating root rot of cotton in seedling stage and application thereof, and belongs to the technical field of agricultural biological prevention and treatment. The bacillus mojavensis HWt disclosed by the invention provides a novel and efficient microorganism for preventing and treating cotton seedling root rot; the bacillus mojavensis wettable powder and the biological fertilizer have high drug effect on cotton root rot, average prevention effect is more than 78.43%, and the bacillus mojavensis wettable powder and the biological fertilizer have broad spectrum; the invention has long lasting period for preventing and curing root rot of cotton in seedling stage, is not easy to generate drug resistance, and has obvious promotion effect on cotton growth.
Description
Technical Field
The invention relates to the technical field of agricultural biological control, in particular to bacillus mojavensis for controlling cotton seedling root rot and application thereof.
Background
The root rot disease of cotton in seedling stage is a comprehensive disease comprising damping-off disease (Rhizoctoni asolani Kuhn), damping-off disease [ Pythium aphanidermatum (eds.) Fitz ], anthracnose [ Colletotrichum gossypii Southw ], red rot disease [ Fusarium moniliforme (Var.) intermedium Neish et Leggett ] and black root rot disease [ Thilaviopsis basicola (Berk. Et Br.) Ferravis ], and the occurrence of Sinkiang cotton damping-off disease is increasingly serious due to factors such as continuous cropping throughout the year, extreme low-temperature weather frequency in early spring and the like.
The prevention and treatment of the root rot of cotton in the seedling stage are mainly performed by measures such as chemical pesticide spraying, coating and the like, but the chemical pesticide has the defects of environmental pollution, drug resistance and the like, and the current country greatly pushes the application policy of chemical fertilizer and pesticide decrement, so that the biological pesticide is used for preventing and treating the cotton seedling stage to meet the national requirements, and has the advantages of environmental friendliness, difficult drug resistance and the like, and needs to be greatly developed and pushed.
Bacillus (sp) is one of the most widely used antagonistic bacteria. Has the characteristics of wide distribution, easy culture, strong stress resistance, storage resistance and the like, and is ideal biocontrol microorganism.
At present, bacillus used for controlling crop diseases mainly comprises bacillus subtilis, bacillus amyloliquefaciens, bacillus megaterium and the like, and no report on the prevention and control of cotton seedling root rot diseases by the bacillus mojavensis is found through searching.
Therefore, the provision of a bacillus mojavensis for preventing and treating root rot of cotton at seedling stage and application thereof are problems to be solved urgently by those skilled in the art.
Disclosure of Invention
In view of the above, the invention provides a bacillus mojavensis for preventing and treating root rot of cotton at seedling stage and application thereof, and the bacillus mojavensis has the advantages of fast propagation, wide sterilization spectrum and the like.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
bacillus mojavensis Bacillus mojavensis HWt with the preservation number of CGMCC No.18941 is preserved in China general microbiological culture Collection center (CGMCC), and is named as Bacillus mojavensis Bacillus mojavensis in China academy of sciences of China, national academy of sciences of China, north China, chenglu No.1, beijing, chaoyang, and the preservation date of the Bacillus mojavensis is 11 and 12 days in 2019.
Further, the bacillus mojavensis HWt is applied to prevention and treatment of cotton seedling root rot.
Further, the wettable powder prepared by using the bacillus mojavensis HWt.
Further, the preparation method of the wettable powder comprises the following steps:
(1) Selecting a strain HWt, wherein a single strain falls on an LB slant culture medium, and culturing for 24 hours at 30 ℃ to obtain an activated strain; inoculating the activated strain into 100ml of LB liquid medium, and culturing for 24 hours at 30 ℃ and a shaking table rotation speed of 150rpm to obtain seed liquid;
the LB liquid medium is 5g of yeast extract, 10g of peptone, 5g of NaCl, 1000ml of water and pH 7.4-7.6;
(2) Inoculating the seed liquid into a saccharose yeast powder soaking culture medium according to the volume ratio of 1-3%, and fermenting and culturing for 72h at the temperature of 28 ℃ and the rotation speed of a shaking table of 150rpm to obtain fermentation liquor;
(3) Filtering the fermentation liquor, and spray-drying to obtain bacillus mojavensis raw powder;
(4) The wettable powder is prepared by 75% of bacillus mojavensis raw powder, 10% of filler diatomite, 15% of dispersing agent, wetting agent and stabilizing agent.
Further, the wettable powder is used for dressing cotton seeds.
Diluting the Bacillus mojavensis HWt34 fermentation liquor with water until the number of viable cells is 10 8 cfu/mL, soaking cotton seeds for one hour before sowing, and after airing, dressing the seeds with bacillus mojavensis wettable powder to achieve the aim of preventing the occurrence of root rot in the cotton seedling stage.
Further, the biological fertilizer prepared from the wettable powder and sheep manure is prepared by mixing 1kg of bacillus mojavensis wettable powder and 1 ton of sheep manure. And uniformly spreading the biological fertilizer serving as a base fertilizer before soil preparation according to 2 tons per mu.
Further, the wettable powder is applied to prevention and treatment of cotton seedling root rot.
Compared with the prior art, the invention discloses bacillus mojavensis for preventing and treating cotton seedling root rot and application thereof, and provides a novel and efficient microorganism for preventing and treating cotton seedling root rot; the bacillus mojavensis wettable powder and the biological fertilizer have high drug effect on cotton root rot, average prevention effect is more than 78.43%, and the bacillus mojavensis wettable powder and the biological fertilizer have broad spectrum; the invention has long lasting period for preventing and curing root rot of cotton in seedling stage, is not easy to generate drug resistance, and has obvious promotion effect on cotton growth.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present invention, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art.
FIG. 1 is a phylogenetic tree of strain HWt34 obtained according to the invention from a 16S r DNA sequence;
FIG. 2 is a phylogenetic tree of strain HWt34 obtained according to the gyrA gene sequence of the present invention;
FIG. 3 is a drawing showing a plate counter test in example 3 of the present invention;
wherein A, B is two replicates of inoculating Bacillus mojavensis HWt with toothpick at 2cm from the edge of the dish; c is a blank.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
EXAMPLE 1 isolation screening of Bacillus mojavensis HWt34
(1) Isolation of Strain HWt34
Strain HWt was isolated from the soil of the stoneware cotton fields by the institute of plant protection, academy of agricultural sciences, xinjiang. The stone wool Tian Tuyang is collected and brought back to a laboratory for separation and purification by a soil dilution method. And screening biocontrol bacteria by taking cotton damping off as a target and adopting a flat plate counter method, a potting test method and a field cell test. From the strain, a strain with obvious prevention and treatment effect on cotton damping off is screened out, and the strain is named HWt34.
(2) Classification and identification of Strain HWt34
(1) Identification of Classification Using 16S r DNA sequences
Using the genomic DNA of HWt as a template and bacterial universal primers 27F and 1492R for PCR amplification to obtain a PCR amplification product, wherein the primer sequences are as follows:
27F：5’-AGAGTTTGATCMTGGCTCAG-3’；SEQ ID NO.1；
1492R：5’-TACGGYTACCTTGTTACGACTT-3’；SEQ ID NO.2；
wherein M represents A/C; y represents C/T.
The reaction system was 2 XTaq Master Mix 25. Mu.L, 27F (10 ppm) and 1492R (10 ppm) each 1. Mu.L, DNA template 1. Mu.L, and ddH was supplemented 2 O22. Mu.L. The PCR amplification condition is pre-denaturation at 94 ℃ for 5min; denaturation at 94℃for 30s, annealing at 54℃for 30s extension at 72℃for 1.5min,35cycles; extending at 72deg.C for 10min. The PCR product was sequenced by Beijing Ding Guo Changsheng biotechnology Co., ltd, to give the 16S r DNA sequence (SEQ ID NO. 3) of the HWt strain. The measured sequence is subjected to BLAST on-line homology comparison through NCBI, a standard strain with higher homology is selected as a reference object, and the result shows that the homology of the strain HWt and 16S r DNA of bacillus reaches 99%; constructing a phylogenetic tree, and the result is shown in figure 1; HWt34 together with Bacillus, HWt is described as belonging to the genus Bacillus.
(2) Identification and classification by using gyrA gene sequence
Performing PCR amplification by taking HWt genomic DNA as a template and gyrA-42F and gyrA-1066R as primers to obtain a PCR amplification product; wherein the sequences of the gyrA-F and gyrA-R primers are as follows:
gyrA-42F：5’-CAGTCAGGAAATGCGTACGTCCTT-3’；SEQ ID NO.4；
gyrA-1066R：5’-CAAGGTAATGCTCCAGGCATTGCT-3’；SEQ ID NO.5；
the gyrA reaction system was 2×Taq Master Mix 25. Mu.L, gyrA-42F (10 μm) and gyrA-1066R (10 μm) each 1. Mu.L, DNA template 1. Mu.L, and dd H was supplemented 2 O22. Mu.L. The PCR amplification condition is pre-denaturation at 94 ℃ for 5min; denaturation at 94℃for 30s, annealing at 54℃for 30sExtending at 72℃for 1.5min,35cycles; extending at 72deg.C for 10min. The PCR product was sequenced by Beijing Ding Guo Changsheng biotechnology Co., ltd, to obtain the gyrA gene sequence (SEQ ID No. 6) of the HWt strain. And (2) carrying out BLAST on-line homology alignment on the gyrA gene sequence of the HWt strain at NCBI, and constructing a phylogenetic tree by utilizing MEGA software, wherein the result is shown in figure 2. As a result, HWt was found to have the highest gyrA gene sequence homology with Bacillus mojavensis, and HWt strain was polymerized with Bacillus mojavensis, indicating that HWt is Bacillus mojavensis (Bacillus mojavensis).
From the results of the comparative analysis of the 16S rDNA and gyrA gene sequences, it was found that the strain HWt belongs to Bacillus mojavensis (Bacillus mojavensis).
Example 2 preparation of HWt34 wettable powders
(1) Activating strains: activating the preserved strain HWt on LB plate medium (comprising 5g of yeast extract, 10g of peptone, 5g of NaCl, 15g of agar powder and 1000ml of water, pH 7.4-7.6) at 30 ℃, and culturing the single colony on LB slant medium at 30 ℃ for 24 hours to obtain an activated strain;
(2) Preparing seed liquid: preparing an LB liquid culture medium (the components and weight ratio thereof are that tryptone 10g, yeast extract 5g, sodium chloride 5g and water 1000 ml) according to a conventional method, filling 100ml of LB culture solution into a 250ml triangular flask, sterilizing under high-pressure damp-heat, after the temperature is reduced to room temperature, inoculating an inoculating loop of the activated strain in the step (1) into each flask, and carrying out shaking culture for 24 hours at the temperature of 30 ℃ and the rotating speed of a shaking table of 150rpm to obtain seed liquid;
(3) Preparation of saccharose yeast powder soaking culture medium: adding 2.5% of sucrose, 2.5% of yeast extract powder and 0.5% of dipotassium hydrogen phosphate into water according to weight percentage, and uniformly mixing to obtain a sucrose yeast extract powder culture medium; split charging into 500ml triangular bottles, wherein each bottle contains 200ml of liquid; sterilizing the saccharose yeast powder soaking culture medium at 121 ℃ for 25 minutes;
(4) Inoculating the seed liquid into a sucrose and yeast powder soaking culture medium according to the volume ratio of 1-3%, and fermenting and culturing for 72h at the temperature of 28 ℃ and the rotation speed of a shaking table of 150rpm to obtain fermentation liquor;
(5) Filtering the fermentation liquor, and spray-drying to obtain bacillus mojavensis raw powder;
(6) The wettable powder is prepared from 75% of bacillus mojavensis raw powder, 10% of filler diatomite, 6.8% of dispersing agent, 5.1% of wetting agent, 3.0% of stabilizing agent and 0.1% of protective agent.
EXAMPLE 3 inhibition of bacterial growth of Ramophila mojalis HWt34 on Ramophila gossypii
The inhibition test of bacillus mojavensis HWt on the growth of the cotton bacterial growth by bacillus mojavensis was carried out in plant disease laboratory of plant protection institute of academy of agricultural sciences in Xinjiang 3 month 2019.
Test rhizoctonia solani: rhizoctonia solani strains were collected from Xinjiang cotton fields, isolated and purified in the laboratory and identified as Rhizoctonia solani (Rhizoctoni asolani), and the pathogenicity assay was shown to be strongly pathogenic.
Plate confrontation test:
rhizoctonia solani is activated in a PDA culture medium, cultured for 3 days, then punched in a colony edge area by a puncher (O=6mm) to prepare a bacterial cake, the bacterial cake is inoculated in the center of the PDA culture medium by a crisscross method, and bacillus mojavensis HWt activated in the step (1) of the example 2 is inoculated at a position 2cm away from the edge of a culture dish by a toothpick. Setting a blank control (without inoculating the strain HWt), culturing at constant temperature of 28 ℃, measuring the control growth amount (colony radius) and the treatment growth amount (inhibition growth radius after HWt inoculation) of the cotton bacterial wilt when the blank control is about to grow on the whole culture dish, and calculating the antibacterial rate:
antibacterial ratio (%) = (control growth amount-treatment growth amount)/control growth amount×100
The results are shown in Table 1, FIG. 3: the inhibition rate of the bacillus mojavensis to the cotton bacterial wilt reaches more than 80 percent; transparent bacteriostatic band width is 8.5 mm; the bacillus mojavensis HWt has obvious inhibition effect on rhizoctonia solani.
TABLE 1 antagonism test of HWT34 Strain against Ralstonia gossypii
| Strain name | Control (mm) | Treatment (mm) | Bacteriostasis rate (%) |
| HWt34 | 45 | 7 | 84.44 |
EXAMPLE 4 test of the inhibition of Bacillus mojavensis HWt34 against two other important disease pathogens of cotton
Experiments were performed in the plant disease laboratory of the institute of plant protection, academy of agricultural sciences, xinjiang, 3 months 2019.
Test of disease pathogenic fungi: verticillium dahliae and Fusarium oxysporum are all separated and stored by plant protection institute of Xinjiang academy of agricultural sciences.
Plate assay:
the pathogenic fungi to be tested were first activated on PDA plates, cultured for 3-7 days, then perforated with a punch (o=6mm) to form a cake, the cake was transferred to the center of the new PDA medium by the crisscross method, and the activated bacillus mojavensis HWt in step (1) of example 2 was inoculated with a toothpick at a distance of 2cm from the edge of the dish. A blank control was set. Culturing at 25deg.C for 7-15 days, and observing and recording radius and width of antibacterial zone of control pathogenic bacteria when they grow to the edge of culture dish. The "++ + +" indicates that the HWt strain has strong antibacterial effect, and the antibacterial band width is more than 9.0mm; the "++" is used for indicating that the bacteriostasis of the HWt strain is medium strength, and the width of the bacteriostasis zone is 3.0-9.0mm; the "+" indicates that the HWt strain has weaker antibacterial activity, and the antibacterial band width is less than 3.0mm. The results are shown in Table 2.
TABLE 2 inhibition of two important pathogenic fungi by HWT34 Strain
| Fusarium oxysporum | Verticillium dahliae (Fr.) Kummer |
| ++ | ++ |
The results in Table 2 show that Bacillus mojavensis HWt has moderate inhibition to Verticillium dahliae and Fusarium oxysporum; the bacillus mojavensis HWt has the biocontrol potential for preventing and controlling cotton wilt.
EXAMPLE 5 control Effect of Bacillus mojavensis HWt34 on cotton damping off
Microbial agent: HWt34 wettable powder prepared in example 2; diluted 10-fold with water. Blank control: and (5) clean water.
The test is carried out in a kuerle test field in Xinjiang, a land block with serious disease occurrence of cotton damping off is selected, a cotton variety is selected from Xinlun No. 66, and 50 times of water diluent of the HWt wettable powder prepared in the example 2 is used for seed soaking for half an hour before sowing; each treatment was repeated 3 times with half an hour of soaking in clear water as a blank. Area per cell 20m 2 . The emergence rate, incidence rate, disease index and control effect were examined at the cotton seedling stage, and the results are shown in Table 3.
Table 3 Bacillus mojavensis HWt34 control effect on cotton damping off
| Treatment of | Emergence rate of seedlings | Incidence of disease | Index of disease condition | Prevention and control effect |
| HWt34 seed soaking | 84.81％ | 6.60％ | 17.33 | 78.43％ |
| Blank control | 67.56％ | 30.6％ | 30.00 | — |
The results in Table 3 show that when the disease index of the blank control cotton damping-off is 30, the disease index of the seed soaking treatment of the bacillus mojavensis HWt is 17.33, and the effect of preventing and treating the cotton damping-off can reach 78.43%. The bacillus mojavensis HWt and the wettable powder thereof have good control effect on cotton damping-off.
EXAMPLE 6 test of control Effect of Bacillus mojavensis wettable powder and biological Fertilizer on root rot of cotton in seedling stage
The test is carried out in a Korla test field of a plant protection institute of Xinjiang academy of sciences, a land block with serious root rot disease occurrence of cotton in a seedling stage is selected, and 2 tons of biological fertilizer (1 kg of bacillus mojavensis wettable powder and 1 ton of sheep manure are mixed to prepare the biological fertilizer) are uniformly spread before soil preparation per mu of land. The cotton variety is 66 on Xinlun, the Bacillus mojavensis fermentation liquor of the example 2 is applied before sowing to immerse cotton seeds for one hour, the seeds are mixed with the Bacillus mojavensis wettable powder after being dried in the air, and then sowing is carried out, and the area of a test field is 25 mu. The incidence of cotton fields was investigated by 10-point sampling method at cotton seedling stage, the control effect was calculated, and the results were shown in Table 4 with neighboring cotton fields as a control.
TABLE 4 prevention effect of Bacillus mojavensis biological fertilizer on cotton root rot
| Treatment of | Incidence of disease | Prevention and control effect |
| Biological fertilizer | 3.4％ | 81.72％ |
| Control | 18.6％ | — |
The results in Table 4 show that the incidence rate of the root rot of the blank control cotton is 18.6%, the incidence rate of the comprehensive treatment by using the biological fertilizer is 3.4%, and the effect of the biological fertilizer on preventing and treating the root rot of the cotton can reach 81.72%. The bacillus mojavensis HWt34 wettable powder and the biological fertilizer thereof disclosed by the invention have good prevention and treatment effects on cotton root rot.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Sequence listing
<110> institute of biotechnology of nuclear technology of the national academy of agricultural sciences of Xinjiang (institute of biotechnology of Uygur autonomous region of Xinjiang) institute of plant protection of the national academy of agricultural sciences of Xinjiang
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ggaccggatt tcccgactgc cggacaaatt ttaggcagaa gcggaatccg caaggcatat 660
gaatccggaa gaggttctat tacgattcgg gcaaaagcag aaattgaaga aacttcttca 720
gggaaagaaa gaattttagt aacagaactc ccttatcagg tgaataaagc gcgtctaatt 780
gagaagattg ctgatcttgt cagggataag aaaattgaag gaataactga tttgcgtgat 840
gaatctgacc gtaacggtat gcggattgtc attgaaatca gaagagatgc caatgcgcat 900
gttatcctga acaacctgta caaacagaca gccctgcaaa cctccttcgg gattaatatg 960
ctggcgcttg ttgacggtga accgaaggta ttaagtttaa gcatgtggg 1009
Claims (7)
1. The bacillus mojavensis Bacillus mojavensis HWt is characterized by having a preservation number of CGMCC No.18941.
2. Use of bacillus mojavensis HWt as defined in claim 1 for controlling root rot of cotton in seedling stage.
3. A wettable powder made using bacillus mojavensis HWt as defined in claim 1.
4. A method of preparing the wettable powder of claim 3, comprising the steps of:
(1) Selecting a strain HWt, wherein a single strain falls on an LB slant culture medium, and culturing for 24 hours at 30 ℃ to obtain an activated strain; inoculating the activated strain into 100ml of LB liquid medium, and culturing for 24 hours at 30 ℃ and a shaking table rotation speed of 150rpm to obtain seed liquid;
(2) Inoculating the seed liquid into a saccharose yeast powder soaking culture medium according to the volume ratio of 1-3%, and fermenting and culturing for 72h at the temperature of 28 ℃ and the rotation speed of a shaking table of 150rpm to obtain fermentation liquor;
(3) Filtering the fermentation liquor, and spray-drying to obtain bacillus mojavensis raw powder;
(4) The wettable powder is prepared by 75% of bacillus mojavensis raw powder, 10% of filler diatomite, 15% of dispersing agent, wetting agent and stabilizing agent.
5. The wettable powder of claim 3 or 4 for dressing cotton seeds.
6. The biological fertilizer prepared by the wettable powder of claim 3 or 4, which is characterized in that 1kg of bacillus mojavensis wettable powder is mixed with 1 ton of sheep manure to prepare the biological fertilizer.
7. The use of the wettable powder according to claim 3 or 4 for controlling root rot of cotton in seedling stage.
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| WO2024017799A1 (en) * | 2022-07-20 | 2024-01-25 | Université de Liège | Biological control agent |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105441366A (en) * | 2015-12-31 | 2016-03-30 | 中国农业大学 | Application of Bacillus methylotrophicus ZBL-1 in control of cotton verticillium wilt |
| CN108641981A (en) * | 2018-05-17 | 2018-10-12 | 十堰市农业科学院 | One plant of Biocontrol Bacillus identification and application |
| CN109152367A (en) * | 2016-02-09 | 2019-01-04 | 巴斯夫欧洲公司 | Mixture and composition comprising bacillus genus bacterial strain or its metabolin and other biological pesticides |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105441366A (en) * | 2015-12-31 | 2016-03-30 | 中国农业大学 | Application of Bacillus methylotrophicus ZBL-1 in control of cotton verticillium wilt |
| CN109152367A (en) * | 2016-02-09 | 2019-01-04 | 巴斯夫欧洲公司 | Mixture and composition comprising bacillus genus bacterial strain or its metabolin and other biological pesticides |
| CN108641981A (en) * | 2018-05-17 | 2018-10-12 | 十堰市农业科学院 | One plant of Biocontrol Bacillus identification and application |
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